Traditional Chinese medicine composition for treating lymphedema

Abstract

The invention provides a traditional Chinese medicine composition for treating lymphedema, and relates to the technical field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine composition comprises raw radix astragali, cortex periplocae, raw bighead atractylodes rhizome, Chinese angelica, pericarpium arecae, peach kernel, safflower, ligusticum wallichii, red peony root, earthworm, caulis spatholobi, spina gleditsiae, beautiful sweetgum fruit, leech, elecampane and szechwan chinaberry fruit. All the medicines are combined to achieve the effects of tonifying qi, strengthening the spleen, warming yang, inducing diuresis, reducing phlegm and activating blood. The traditional Chinese medicine composition can effectively inhibit inflammatory response and relieve endothelial cell injury; the traditional Chinese medicine composition has obvious edema eliminating and anti-inflammatory effects on mice with lymphedema, can obviously relieve the fibrosis degree of the mice with lymphedema and improve the fat deposition condition, and has an obvious treatment effect on the mice with lymphedema. Meanwhile, the traditional Chinese medicine composition disclosed by the invention can be used for remarkably improving the total treatment effective rate of a patient with lymphedema, remarkably relieving the limb swelling degree of the patient and relieving the pain of the patient, and provides conditions for preparing a new medicine for treating lymphedema.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine pharmaceutical preparation, and in particular to a traditional Chinese medicine composition for treating lymphedema. Background Art

[0002] Secondary lymphedema is a chronic, progressive and disabling disease. It is a pathological protein accumulation in the interstitial space caused by various factors that block the return of lymph or the dysfunction of lymphatic transport. It is mainly manifested by obvious thickening and fibrosis of skin tissue, fat deposition and lymphatic stasis, and is prone to cellulitis or erysipelas. The swelling and subsequent hardening of the affected area can lead to disfigurement, as well as decreased mobility and function, which seriously affects the patient's physical and mental health. Lymphedema is a common complication after tumor surgery, often secondary to breast cancer, sarcoma, gynecological cancer and malignant melanoma, among which lymphedema after breast cancer surgery is the most common. Lymphedema can occur after any late treatment of cancer that affects lymph node drainage. It is reported that lymphedema occurs within a few days after breast cancer treatment, up to 30 years, and 80% of patients develop the disease within 3 years after surgery. Upper limb lymphedema most often occurs after breast cancer, and lower limb lymphedema most often occurs in uterine cancer, prostate cancer, lymphoma or melanoma.

[0003] There are three main types of treatment methods commonly used in Western medicine at present, namely surgical treatment, related drug treatment and conservative treatment. In the early stage, conservative treatment means such as eliminating stagnant lymph fluid and preventing the regeneration of lymphatic effusion are used. In the late stage, the purpose is to surgically remove the diseased tissue that cannot be restored or to treat localized lymphatic obstruction with shunt surgery. However, the overall treatment effect of Western medicine is not satisfactory. In recent years, with the continuous promotion and application of traditional Chinese medicine, many researchers have found that traditional Chinese medicine has significant therapeutic effects in improving the clinical symptoms of patients with lymphedema and improving the quality of life of patients with lymphedema. The present invention aims to provide a Chinese medicine composition for the treatment of lymphedema with small side effects, significant efficacy, and can alleviate patient symptoms and improve the function of affected limbs. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a Chinese medicine composition for treating or improving lymphedema.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a traditional Chinese medicine composition for treating lymphedema. The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 20-40 parts of raw astragalus, 5-25 parts of cortex perillae, 10-30 parts of raw atractylodes, 5-20 parts of angelica, 5-20 parts of tangerine peel, 5-20 parts of peach kernel, 5-20 parts of safflower, 5-20 parts of ligusticum chuanxiong, 5-20 parts of red peony root, 1-15 parts of earthworm, 20-40 parts of millettia reticulata, 5-20 parts of thorn of Gleditsia sinensis, 5-20 parts of Fructus liquidaminae, 1-15 parts of leech, 5-20 parts of costusroot and 5-20 parts of toosendan fruit.

[0009] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 25-35 parts of raw astragalus, 10-20 parts of perilla bark, 15-25 parts of raw atractylodes, 5-15 parts of angelica, 5-15 parts of tangerine peel, 10-20 parts of peach kernel, 10-20 parts of safflower, 10-20 parts of ligusticum chuanxiong, 5-15 parts of red peony root, 5-15 parts of earthworm, 25-35 parts of millettia reticulata, 10-20 parts of gleditsia thorn, 10-20 parts of rhizoma smilacis Glabrae, 1-10 parts of leech, 10-20 parts of costusroot, and 5-15 parts of toosendan fruit.

[0010] Furthermore, the Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of raw astragalus, 15 parts of cortex berberis, 20 parts of raw atractylodes, 10 parts of angelica, 10 parts of tangerine peel, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 10 parts of red peony root, 6 parts of earthworm, 30 parts of millettia reticulata, 15 parts of gleditsia thorn, 15 parts of rhizoma smilacis, 3 parts of leech, 12 parts of costusroot, and 10 parts of toosendan fruit.

[0011] The Chinese medicine composition provided by the present invention is used to prepare a medicine for treating or improving lymphedema. The Chinese medicine composition is prepared into a decoction, pills, oral liquid, granules, powder or other pharmaceutically acceptable oral dosage forms according to conventional pharmaceutical methods in pharmacy and with medically acceptable adjuvants.

[0012] Raw Astragalus: Astragalus is sweet and slightly warm in nature. It enters the spleen and lung meridians and is an important medicine for replenishing qi. In clinical application, it is usually divided into three types: raw, honey-roasted, and bran-fried. Their medicinal effects are different. Raw Astragalus is mostly used to consolidate the surface, relieve sores, and promote diuresis. Astragalus contains active substances such as polysaccharides, coumarins, flavonoids, saponins, trace folic acid, and several vitamins. Modern pharmacological studies have shown that Astragalus has multiple pharmacological effects such as enhancing immunity and antiviral ability, promoting body metabolism, regulating blood pressure and blood sugar, anti-oxidation, anti-aging, and strengthening the heart.

[0013] Perennial Herb: Spicy and bitter in taste, warm in nature, and effective for the liver, kidney, and heart meridians; has diuretic, anti-edema, rheumatism, and bone strengthening effects, and is used to treat lower limb edema, palpitations and shortness of breath, wind-cold and dampness, and soreness of the waist and knees; Perennial Herb mainly contains chemical components such as steroids, cardiac glycosides, triterpenes, aldehydes, as well as oligosaccharides, small molecule fatty acids, flavonoids, etc., and has multiple pharmacological effects such as cardiotonic, anti-tumor, anti-inflammatory, and immunomodulatory.

[0014] Raw Atractylodes macrocephala: enters the spleen and stomach meridians, tastes bitter, sweet and warm in nature. Functions: invigorate the spleen and replenish qi, dry dampness and promote diuresis, stop sweating, and stabilize the fetus; commonly used for spleen deficiency, poor appetite, diarrhea, loose stools, and restless fetuses; its main chemical components include volatile oils, polysaccharides, and lipids, which have anti-tumor, anti-inflammatory, and digestive system regulation effects.

[0015] Angelica: sweet and spicy in taste, neutral in nature, enters the liver, heart and spleen meridians, has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, moistening the intestines and promoting bowel movements; often used for blood deficiency and sallow complexion, dizziness and palpitations, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to deficiency and cold, rheumatism and pain, injuries from falls, carbuncles, ulcers, dry intestines and constipation and other symptoms; Angelica mainly contains volatile oils, organic acids, polysaccharides, flavonoids, amino acids, and various trace elements and other effective ingredients, has antioxidant, liver protection, anti-inflammatory, hematopoietic promotion, liver protection and kidney strengthening effects and other effects.

[0016] Phellodendron chinense: spicy in taste, slightly warm in nature, enters the spleen, stomach, large intestine and small intestine meridians; has the effects of promoting qi and relieving fullness, promoting water circulation and reducing swelling; used for dampness blocking qi stagnation, abdominal distension, uncomfortable bowel movements, edema, athlete's foot, and difficulty urinating; Phellodendron chinense mainly contains arecoline, paraarecoline, etc., and is mainly used clinically for the treatment of digestive system diseases.

[0017] Peach kernel: bitter, sweet, neutral, and enters the heart, liver, and large intestine meridians; it promotes blood circulation and removes blood stasis, moistens the intestines and promotes bowel movements, and relieves cough and asthma; it is used for amenorrhea, dysmenorrhea, abdominal masses, lung abscesses, intestinal abscesses, injuries from falls, dry intestines and constipation, and cough and asthma; peach kernels mainly contain glycosides, fatty oils, proteins, amino acids, volatile oils, aromatic glycosides, flavonoids and their glycosides, sterols and their glycosides, etc., which have multiple pharmacological effects such as promoting blood circulation and removing blood stasis, anti-cancer, anti-inflammatory, and neuroprotection.

[0018] Safflower: spicy, warm in nature, enters the heart and liver meridians; has the effects of promoting blood circulation, relieving menstruation, dispersing blood stasis and relieving pain; used for amenorrhea, dysmenorrhea, lochia retention, chest pain, abdominal pain due to blood stasis, sores and swelling; contains chemical components such as flavonoids, steroids, phenolic acids, diols, lignans, chalcones, alkynes and volatile oils, among which safflower yellow is the main active ingredient.

[0019] Chuanxiong: spicy in taste, warm in nature, enters the liver, gallbladder and pericardium meridians; has the effects of promoting blood circulation and qi, dispelling wind and relieving pain, and is used to treat menstrual disorders, amenorrhea, dysmenorrhea, chest and flank pain, swelling and pain due to falls, rheumatic pain and other diseases; Chuanxiong contains active ingredients such as chuanxiongzine, sodium ferulate, and chuanxiong lactone, which have pharmacological effects such as promoting blood circulation and removing blood stasis, sedation and analgesia, and inhibiting the release of oxygen free radicals.

[0020] Red peony root: slightly cold in nature, bitter in taste; enters the liver and spleen meridians; has the effects of clearing away heat and cooling blood, promoting blood circulation and removing blood stasis; used for symptoms such as heat entering the blood, warm toxicity causing spots, liver depression and flank pain, and edema and ulcers; red peony root contains a variety of effective chemical ingredients such as red peony total glucosides, gallic acid, red peony total flavonoids and neolignan glycoside compounds; modern pharmacological studies have shown that red peony root has neuroprotective, anti-depressant, blood sugar and blood lipid regulation, anti-inflammatory and antibacterial, anti-tumor, antioxidant and immune regulation effects.

[0021] Earthworm: salty in taste, cold in nature; enters the liver, spleen, and bladder meridians; has the effects of clearing away heat and calming the nerves, unblocking the meridians, relieving asthma, and promoting diuresis; used for symptoms such as wandering nature, unblocking the meridians and activating the collaterals; modern research shows that it has anticonvulsant, antipyretic, sedative, antithrombotic, anticoagulant, antihypertensive, analgesic, anti-inflammatory, antiasthmatic, anti-tumor, diuretic, and immune-enhancing effects.

[0022] Millettia reticulata: bitter and sweet in taste, warm in nature, enters the liver and kidney meridians; has the effects of promoting blood circulation, tonifying blood, regulating menstruation and relieving pain, relaxing muscles and tendons; used to treat irregular menstruation, dysmenorrhea, rheumatic pain, numbness and paralysis, blood deficiency and chlorosis; Millettia reticulata mainly contains chemical components such as flavonoids, phenolic acids, sterols, triterpenes, lignans, anthraquinones and trace elements; clinically it is often used to improve the blood system, protect the cardiovascular system, anti-tumor, bidirectionally regulate tyrosinase, anti-oxidation, etc., prevention and treatment of diseases such as leukopenia, thrombocytopenia and red blood cell pancytopenia and aplastic anemia.

[0023] Gleditsia sinensis thorns: spicy, warm in nature, enter the liver and stomach meridians; have the effects of reducing swelling, expelling toxins, draining pus, and killing insects; mainly treat carbuncle in the early stage or pus that has not broken out, external treatment of scabies and leprosy; Gleditsia sinensis thorns contain flavonoids, lactones, coumarins or their glycosides, sterols, triterpenes, classifications, organic acids, reducing sugars, triterpenoid saponins and other effective ingredients; mainly have anti-tumor, antibacterial and bactericidal, anti-inflammatory, antiviral, immunomodulatory, anti-allergic, anticoagulant, antioxidant, lipid-lowering and other pharmacological effects.

[0024] Rhizoma Lulutong: bitter in taste, neutral in nature, and is used for the liver and kidney meridians; it has the effects of dispelling wind, activating collaterals, promoting diuresis, and dredgeing menstruation; it is used for joint pain, numbness and spasm, edema, oligolactosis, amenorrhea, etc.; the main components of Rhizoma Lulutong include xylindole, gallic acid, Rhizoma Lulutong A and other terpenes, aliphatics, and aromatics, which have the pharmacological effects of anti-inflammatory and detumescence, inhibition of pathogenic microorganisms, protection of nerves, anti-oxidation, and anti-tumor.

[0025] Leeches: salty and bitter in taste, neutral in nature, belonging to the liver meridian; have the effects of breaking blood and promoting menstruation, removing blood stasis and eliminating symptoms. Used for blood stasis, amenorrhea, lumps, hemiplegia due to stroke, and traumatic injuries; leeches contain protein and 17 kinds of amino acids; have multiple pharmacological effects such as anticoagulation, anti-inflammatory, anti-cancer, anti-fibrosis, and promoting skin wound healing.

[0026] Costus root: pungent and bitter in taste, warm in nature; enters the spleen, stomach, large intestine, triple burner, and gallbladder meridians; has the effects of promoting qi and relieving pain, strengthening the spleen and digesting food; clinically it is mainly used for gastrointestinal diseases such as spleen and stomach qi stagnation, diarrhea, tenesmus, etc.; modern pharmacological research shows that costus root has certain activities in anti-cancer, immunity, and anti-inflammatory aspects in addition to relieving smooth muscle spasms, lowering blood pressure, and antibacterial effects.

[0027] Toosendan fruit: bitter, cold; enters the liver, small intestine, and bladder meridians; soothes the liver and relieves heat, promotes qi circulation and relieves pain, and kills insects; used for liver depression and fire transformation, chest and flank, abdominal distension and pain, hernia pain, and abdominal pain caused by worms; Toosendan fruit mainly contains active ingredients such as volatile oils, lignans, organic acids, flavonoids, limonoids, etc., which have anti-inflammatory, analgesic, and neuroprotective effects on the central nervous system, and also have antioxidant, anti-tumor, antibacterial, antiviral, and anti-insect pharmacological effects.

[0028] (III) Beneficial effects

[0029] In traditional Chinese medicine, lymphedema is classified as "big foot wind, beriberi, beriberi, elephantiasis, skin water" and other categories. Traditional Chinese medicine believes that the main factors of lymphedema are "deficiency, toxicity, stasis, water, phlegm, and heat". It is divided into deficiency and excess. Deficiency includes qi deficiency and yang deficiency, and excess includes heat toxicity, blood stasis, and water. Among them, spleen and stomach weakness, metabolic disorders, endogenous phlegm and fluid, yang deficiency cannot control water, then the fluid flows to the limbs, and edema occurs, which is the overall pathogenesis of the human body. The local pathogenesis is due to deficiency leading to excess, due to qi deficiency, blood stasis, blood stasis, water stasis and edema. Water stasis turns into heat after a long time, heat toxicity is generated, burning yin and damaging qi and body fluids, yin damage leads to blood stasis, edema becomes more and more lingering and difficult to cure, and then becomes a syndrome of deficiency and excess, cold and heat. Invigorating qi, strengthening spleen, warming yang, promoting water, resolving phlegm, and promoting blood circulation are the general principles for the comprehensive treatment of lymphedema.

[0030] Based on the above treatment rules and following the principle of treatment based on syndrome differentiation in traditional Chinese medicine, the present invention rationally prepares a Chinese medicine composition for treating lymphedema consisting of raw astragalus, cortex rotundus, raw atractylodes, angelica, cortex tangerine peel, peach kernel, safflower, ligusticum chuanxiong, red peony root, earthworm, 30 parts of millettia reticulata, thorn of gleditsia sinensis, rhizoma smilacis asiatica, leech, costusroot and toosendan fruit.

[0031] In the formula, raw Astragalus membranaceus greatly replenishes the body's Qi. When Qi is strong, blood can flow. When Qi is sufficient, water and dampness can be transported and transformed. Therefore, it can remove blood stasis without hurting the body, promote diuresis and reduce swelling. The taste of Cyperus rotundus is bitter and pungent, and it is warm in nature. It is good at promoting diuresis and reducing swelling. The two are the main drugs, and they work together to replenish Qi and remove dampness. Atractylodes macrocephala is sweet and warm to replenish deficiency, and has a specific effect of replenishing Qi and strengthening the spleen. Angelica sinensis has a strong effect of nourishing blood and activating blood circulation, and removes blood stasis without hurting the body. The tangerine peel strengthens the spleen and removes dampness to eliminate the water and dampness flowing into the skin and meridians. The three are the assistant drugs, and while assisting raw Astragalus membranaceus and Cyperus rotundus to promote diuresis, they also have the effects of promoting blood circulation and removing blood stasis, strengthening the spleen and replenishing Qi. Peach kernel, safflower, Chuanxiong, red peony root, earthworm, and Millettia reticulata work together to promote blood circulation, remove blood stasis, and dredge collaterals, unblocking the meridians; Gleditsia sinensis and Rhizoma Smilacis Glabrae warm the meridians and dredge the collaterals, promote diuresis and reduce swelling; Leech removes blood stasis, bad blood, and breaks up accumulations, and is particularly strong in removing blood stasis, removing blood stasis, and dredges the meridians; on the basis of replenishing qi, promoting blood circulation, removing blood stasis, warming the meridians and dispersing cold, and dredges and relieves pain, Chinese medicines such as costus root and Toosendan fruit are given according to syndrome differentiation to promote qi, so that the replenished qi can play its role in transporting and transforming water and dampness. The whole prescription targets "qi deficiency, blood stasis, and water retention", and works together to replenish qi, strengthen the spleen, warm the yang, promote diuresis, resolve phlegm, and promote blood circulation, so that edema can be eliminated.

[0032] According to the results of cell experiments, the Chinese medicine composition of the present invention can effectively inhibit inflammatory reactions, reduce endothelial cell damage, and thus achieve the purpose of effectively treating inflammatory lymphedema. The results of animal experiments further show that the Chinese medicine combination of the present invention can significantly reduce the circumference of the hind limbs of mice with lymphedema, has a significant effect of eliminating edema, and effectively reduces the content of inflammatory factors in serum, and has a significant anti-inflammatory effect; at the same time, it can significantly reduce the degree of fibrosis in mice with lymphedema model and improve fat deposition. The Chinese medicine composition of the present invention has a significant therapeutic effect on mice with lymphedema model.

[0033] At the same time, the Chinese medicine composition of the present invention can significantly improve the total effective rate of treatment of lymphedema patients, significantly reduce the degree of limb swelling of patients, and reduce the pain of patients. The Chinese medicine composition of the present invention can be used to prepare a new drug for treating lymphedema. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 is the increase in the circumference of the hind limbs of mice in each group; among them, compared with the sham operation group, the model group was *P<0.05; compared with the model group, # P<0.05; compared with 1d in each group, △ P<0.05, compared with 7d, & P<0.05.

[0035] Figure 2 is the relative expression of IL-6 in the serum of mice in each group; compared with the model group and sham operation group, *P<0.05, *P<0.01; compared with the model group, # P<0.05, ## P<0.01.

[0036] Figure 3 is the relative expression of TNF-α in the serum of mice in each group; compared with the model group and sham operation group, *P<0.05, **P<0.01; compared with the model group, # P<0.05, ## P<0.01.

[0037] Figure 4 The results of Masson staining of skin tissues of mice in each group.

[0038] Figure 5 Oil red staining results of skin tissue of mice in each group. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Example 1

[0041] A traditional Chinese medicine composition for treating lymphedema, the traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of raw astragalus, 15 parts of cortex rotundifolia, 20 parts of raw atractylodes, 10 parts of angelica, 10 parts of tangerine peel, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 10 parts of red peony root, 6 parts of earthworm, 30 parts of millettia reticulata, 15 parts of gleditsia thorn, 15 parts of rhizoma liquidambar formosana, 3 parts of leech, 12 parts of costusroot, and 10 parts of toosendan fruit.

[0042] The Chinese medicine composition is prepared into decoction, pills, oral liquid, granules, powder or other pharmaceutically acceptable oral dosage forms by conventional pharmaceutical preparation methods and with medically acceptable auxiliary materials.

[0043] Example 2

[0044] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of raw astragalus, 8 parts of Perennial Perilla Cortex, 12 parts of raw Atractylodes macrocephala, 6 parts of Angelica sinensis, 8 parts of Cibotium bark, 6 parts of peach kernel, 7 parts of safflower, 8 parts of Chuanxiong, 5 parts of red peony root, 3 parts of earthworm, 22 parts of Millettia reticulata, 8 parts of Gleditsia sinensis thorn, 5 parts of Fructus Smilacis Glabrae, 3 parts of Leech, 8 parts of Costusroot, and 8 parts of Toosendan Fruit.

[0045] Example 3

[0046] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of raw astragalus, 25 parts of cortex rotundus, 30 parts of raw atractylodes, 20 parts of angelica, 20 parts of tangerine peel, 20 parts of peach kernel, 20 parts of safflower, 20 parts of ligusticum chuanxiong, 20 parts of red peony root, 15 parts of earthworm, 40 parts of millettia reticulata, 20 parts of gleditsia thorn, 20 parts of rhizoma smilacis Glabrae, 15 parts of leech, 20 parts of costusroot, and 20 parts of toosendan.

[0047] Example 4

[0048] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 36 parts of raw astragalus, 22 parts of cortex rotundus, 26 parts of raw atractylodes, 17 parts of angelica, 18 parts of tangerine peel, 18 parts of peach kernel, 14 parts of safflower, 16 parts of ligusticum chuanxiong, 18 parts of red peony root, 10 parts of earthworm, 30 parts of millettia reticulata, 16 parts of gleditsia thorn, 17 parts of rhizoma smilacis, 12 parts of leech, 17 parts of costusroot, and 18 parts of toosendan.

[0049] Example 5

[0050] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of raw astragalus, 14 parts of Perennial Perilla Cortex, 12 parts of raw Atractylodes macrocephala, 10 parts of Angelica sinensis, 8 parts of Cibotium bark, 8 parts of peach kernel, 8 parts of safflower, 8 parts of Chuanxiong, 8 parts of red peony root, 4 parts of earthworm, 22 parts of Millettia reticulata, 8 parts of Gleditsia sinensis thorn, 8 parts of Smilax glabra, 5 parts of leech, 8 parts of costusroot, and 8 parts of Toosendan fruit.

[0051] Example 6

[0052] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of raw astragalus, 10 parts of Perennial Perilla Cortex, 15 parts of raw Atractylodes macrocephala, 5 parts of Angelica sinensis, 5 parts of Cibotium bark, 10 parts of peach kernel, 10 parts of safflower, 10 parts of Chuanxiong, 5 parts of red peony root, 5 parts of earthworm, 35 parts of Millettia reticulata, 20 parts of Gleditsia sinensis, 20 parts of Smilax glabra, 10 parts of Leech, 20 parts of Costusroot lappa, and 15 parts of Toosendan fruit.

[0053] Example 7

[0054] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 35 parts of raw astragalus, 20 parts of Perennial Perilla Cortex, 25 parts of raw Atractylodes macrocephala, 15 parts of Angelica sinensis, 15 parts of Cibotium bark, 20 parts of peach kernel, 20 parts of safflower, 20 parts of Chuanxiong, 15 parts of red peony root, 15 parts of earthworm, 35 parts of Millettia reticulata, 20 parts of Gleditsia sinensis thorn, 20 parts of Smilax glabra, 10 parts of leech, 20 parts of costusroot, and 15 parts of Toosendan fruit.

[0055] Example 8

[0056] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 28 parts of raw astragalus, 12 parts of Perennial Perilla Cortex, 18 parts of raw Atractylodes macrocephala, 10 parts of Angelica sinensis, 8 parts of Cibotium bark, 14 parts of peach kernel, 14 parts of safflower, 14 parts of Chuanxiong, 8 parts of red peony root, 8 parts of earthworm, 26 parts of Millettia reticulata, 16 parts of Gleditsia sinensis, 16 parts of Smilax glabra, 2 parts of leech, 16 parts of costusroot, and 8 parts of Toosendan fruit.

[0057] Example 9

[0058] The difference between this embodiment and embodiment 1 is that the Chinese medicine composition is composed of the following raw materials in parts by weight: 32 parts of raw astragalus, 16 parts of Perennial Perilla Cortex, 22 parts of raw Atractylodes macrocephala, 12 parts of Angelica sinensis, 14 parts of Cibotium bark, 18 parts of peach kernel, 16 parts of safflower, 17 parts of Chuanxiong, 13 parts of red peony root, 13 parts of earthworm, 30 parts of Millettia reticulata, 16 parts of Gleditsia sinensis thorn, 18 parts of Smilax glabra, 7 parts of Leech, 17 parts of Costusroot, and 11 parts of Toosendan fruit.

[0059] Test Example 1

[0060] 1 Experimental Drug

[0061] Drug 1: 30 parts of raw astragalus, 15 parts of Persimmon Cortex, 20 parts of raw Atractylodes, 10 parts of Angelica, 10 parts of Big Belly Cortex, 12 parts of Peach Kernel, 12 parts of Carthamus, 12 parts of Ligusticum chuanxiong, 10 parts of Red Peony Root, 6 parts of Pheretima, 30 parts of Millettia Spatholobi, 15 parts of Gleditsia sinensis, 15 parts of Fructus Smilacis Glabrae, 3 parts of Leech, 12 parts of Aucklandiae, and 10 parts of Toosendan Fructus.

[0062] Medicine 2: 15 parts of Persimmon Cortex, 10 parts of Angelica Sinensis, 10 parts of Big Belly Cortex, 12 parts of Peach Kernel, 12 parts of Carthamus Tinctorius, 10 parts of Red Peony Root, 6 parts of Pheretima, 15 parts of Gleditsia Spinosa, 15 parts of Fructus Smilacis Glabrae, 3 parts of Leech, 12 parts of Aucklandiae, and 10 parts of Toosendan Fructus.

[0063] Medicine 3: 30 parts of raw Astragalus, 15 parts of Persimmon Cortex, 20 parts of raw Atractylodes, 10 parts of Big Belly Cortex, 12 parts of Chuanxiong, 10 parts of Red Peony Root, 6 parts of Pheretima, 30 parts of Millettia Spatholobi, 15 parts of Gleditsia sinensis, 15 parts of Smilax glabra, 12 parts of Aucklandia lappa, and 10 parts of Toosendan Fructus.

[0064] Drug 4: 30 parts of raw astragalus, 20 parts of raw atractylodes, 10 parts of angelica, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 10 parts of red peony root, 6 parts of earthworm, 30 parts of millettia reticulata, 3 parts of leech, 12 parts of costusroot, and 10 parts of toosendan.

[0065] Medicine 5: 30 parts of raw astragalus, 15 parts of cortex rotundus, 20 parts of raw atractylodes, 10 parts of angelica, 10 parts of tangerine peel, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 30 parts of millettia reticulata, 15 parts of gleditsia thorn, 15 parts of rhizoma smilacis, and 3 parts of leeches.

[0066] Weigh each raw material according to the above weight proportions, wash, soak with 3 times deionized water for 20 minutes, then boil for 30 minutes, repeat the boiling for more than 2 times, collect the decoction, concentrate the decoction to 1g / mL (1mL contains 1g of crude drug), and obtain the drug stock solution 1-5 of each formulation, store at 4°C for standby use; when using, dilute each drug stock solution to 5μg / mL with PBS and use it immediately after preparation.

[0067] Lipopolysaccharide (LPS) solution: Prepared to 2.5 μg / mL with PBS.

[0068] 2 Experimental methods

[0069] 2.1 Cell culture

[0070] Human umbilical vein endothelial cells (HUVEC) were cultured in IMDM medium containing 10% fetal bovine serum at 37°C and 5% CO2 in a cell culture incubator. When the cell density reached 80% to 90%, the cells were digested with trypsin for 3 minutes. Under a microscope, the cells were observed to be rounded and the gaps became larger. The digestion was immediately terminated with an appropriate amount of IMDM medium containing 10% fetal bovine serum. The cells were suspended after centrifugation at 1000 rpm for 5 minutes and subcultured at a ratio of 1:2 to 1:3.

[0071] 2.2 Construction and grouping of inflammatory injury cell model

[0072] HUVEC cells were treated with 2.5 μg / mL LPS for 6 hours to construct an inflammatory cell injury model. The experiment was divided into a blank group, a model group (2.5 μg / mL LPS), and experimental groups 1-5 (2.5 μg / mL LPS + 5 μg / mL drugs 1-5).

[0073] 2.3 Cell viability assay

[0074] HUVECs in the logarithmic growth phase were plated at 7×10 3 The cells were inoculated into a 96-well plate at a density of 10 μg / cm2, with 4 replicate wells in each group. The cells were cultured at 37°C and 5% CO2 for 12 hours to allow the cells to adhere to the wall for subsequent experiments. Drugs 1-5 were added to the wells and incubated for 24 hours. Then, 10 μL of CCK-8 working solution was added to each well, incubated in the incubator for 0.5 to 2 hours, gently mixed for 1 minute, and the absorbance at 450 nm was measured with an enzyme-labeled instrument, and the values ​​were recorded and analyzed.

[0075] 2.4ELISA assay to determine the expression of inflammatory factor IL-6

[0076] HUVECs in the logarithmic growth phase were seeded into 96-well plates at a seeding density of 9 × 10 3, set up 3 duplicate wells for each group. Culture cells at 37℃ and 5% CO2 for 12h to allow cells to adhere to the wall. Then add the corresponding drugs respectively, continue to culture for 6h, take 100μL of culture solution, centrifuge at 3000rpm for 10min to remove the mixed particles in the culture solution. Take 10μL of each group of samples and add them to the ELISA plate, then add 40μL of sample diluent to each well, then add 100μL HRP to each well, and incubate at 37℃ for 1h. Discard all the liquid in the well, add 300μL of washing solution to each well for washing, each time for 1min, then discard the liquid, pat the plate dry, and repeat the washing operation. Then add 50μL of substrate A and substrate B to each well in turn, and incubate at 37℃ for 15min. Finally, add 50μL of stop solution to each well, measure the absorbance value at 450nm with an enzyme marker, record the value and analyze.

[0077] 2.5 Statistical analysis

[0078] Statistical analysis was performed using Prism 8.0.2, and one-way analysis of variance was used for comparison among groups. The results are expressed as mean ± standard deviation The data were analyzed using t-test. P < 0.05 was considered statistically significant.

[0079] 3 Results

[0080] 3.1 Toxicity to HUVEC

[0081] The results are shown in Table 1. The cell activities of experimental groups 1-5 were all above 93%, and there was no statistically significant difference among the groups (P<0.05). This result shows that drugs 1-5 have no obvious toxic effects on HUVEC.

[0082] Table 1 Cell activity of experimental groups 1-5

[0083] Grouping Experimental Group 1 Experimental Group 2 Experimental Group 3 Experimental Group 4 Experimental Group 5 Cell activity (%) 96.41±3.01 95.15±2.06 93.05±1.79 98.17±2.73 95.18±3.21

[0084] 3.2 Anti-inflammatory effect on inflammatory-damaged HUVEC

[0085] The results are shown in Table 2. Compared with the blank group, the relative expression of IL-6 in the model group was significantly increased (P<0.05); compared with the model group, the relative expression of IL-6 in experimental groups 1-5 was significantly decreased, with significant differences (P<0.05); among them, the relative expression of experimental group 1 decreased the most, significantly lower than that of experimental groups 2-5 (P<0.05), and there was no significant difference compared with the blank group (P>0.05).

[0086] According to the above results, the Chinese medicine composition of the present invention can effectively inhibit inflammatory response, reduce endothelial cell damage, and thus achieve the purpose of effectively treating inflammatory lymphedema. Each component has synergistic effects and none of them can be missing.

[0087] Table 2 IL-6 expression results of HUVECs damaged by inflammation in experimental groups 1-5

[0088] Grouping Relative expression of IL-6 Blank Group <![CDATA[1.00±0.00 a ]]> Model Group <![CDATA[1.41±0.06 b ]]> Experimental Group 1 <![CDATA[1.01±0.01 a ]]> Experimental Group 2 <![CDATA[1.16±0.05 c ]]> Experimental Group 3 <![CDATA[1.26±0.04 c ]]> Experimental Group 4 <![CDATA[1.20±0.08 c ]]> Experimental Group 5 <![CDATA[1.22±0.06 c ]]>

[0089] Note: The same lowercase letters on the shoulders indicate P>0.05; different lowercase letters on the shoulders indicate P<0.05.

[0090] Test Example 2

[0091] Effects of the Chinese medicine composition of the present invention on lymphedema model mice.

[0092] 1 Materials and methods

[0093] 1.1 Experimental animals

[0094] SPF male C57BL / 6J mice, weighing 20-22 g, aged 7-8 weeks, were adaptively raised for 1 week with free access to food and water, at a temperature of 25°C and a humidity of 50%-60%.

[0095] 1.2 Experimental drugs

[0096] The Chinese medicine composition of the present invention comprises: 30 parts of raw astragalus, 15 parts of cortex perillae, 20 parts of raw atractylodes, 10 parts of angelica, 10 parts of cortex tangerinae, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 10 parts of red peony root, 6 parts of earthworm, 30 parts of millettia reticulata, 15 parts of thorns of gleditsia, 15 parts of fennel, 3 parts of leeches, 12 parts of costusroot and 10 parts of toosendan fruit; each raw material is weighed according to the above parts by weight, washed, soaked in 3 times of deionized water for 20 minutes, then decocted for 30 minutes, the decocting is repeated for more than 2 times, the decoction is collected, the decoction is concentrated to 1g / mL (1mL contains 1g of the raw drug), and stored at 4°C for standby use.

[0097] Positive drug: Furosemide tablets

[0098] 1.2 Construction of mouse hindlimb lymphedema model

[0099] After 1 week of adaptive feeding, healthy C57BL / 6J male mice were anesthetized by intraperitoneal injection of sodium pentobarbital and routine skin preparation and disinfection. 0.1% evans blue 0.1ml was injected subcutaneously into the left paw of the mouse to identify the lymph nodes and lymphatic vessels. After 5-10 minutes, the left inguinal skin was cut circumferentially, the inguinal and popliteal lymph nodes were removed, and the deep lymphatic vessels were destroyed. A silicone ring about 2-3mm wide was fixed to the edge of the incision skin with 6-0 sutures, leaving a gap to prevent skin contraction and healing and block the formation of superficial lymphatic vessels. After 5-7 days, obvious edema appeared in the hind limbs of the mouse, indicating that the model was successful.

[0100] 1.3 Establishment of sham surgery model in mice

[0101] After 1 week of adaptive feeding, 15 healthy C57BL / 6J male mice were anesthetized by intraperitoneal injection of sodium pentobarbital and routine skin preparation and disinfection. Only the left groin skin was circumferentially incised without damaging the lymph nodes and lymphatic vessels. A silicone ring with a width of about 2-3 mm was directly fixed to the skin edge using 6-0 thread. No edema appeared in the hind limbs of the mice.

[0102] 1.4 Group Processing

[0103] The successfully constructed mouse model was divided into a sham operation group, a model group, a positive control group (furosemide tablets, 50 mg / kg), a low-dose group of the Chinese medicine composition of the present invention (referred to as the low-dose group, the Chinese medicine stock solution of the present invention is 0.5 g / kg), and a high-dose group of the Chinese medicine composition of the present invention (referred to as the high-dose group, the Chinese medicine stock solution of the present invention is 1.0 g / kg), with 10 mice in each group. The sham operation group and the model group were given the same dose of normal saline, and each group was gavaged for 14 consecutive days. The changes in edema in the hind limbs of the mice were observed and recorded. After the end of the administration, the mice were killed and the samples were collected.

[0104] 1.5 Observation indicators

[0105] 1.5.1 Measurement of hind limb circumference

[0106] The mice were fixed on a dissecting board under anesthesia. After the hair was prepared, the circumference of the mouse 2 mm above the knee was measured with a non-elastic micro tape measure. The measurement was repeated 3 times and the average value was taken.

[0107] All animals were measured before modeling, immediately after modeling, on the 7th day of drug administration, and on the 14th day of drug administration. Each measurement was repeated 3 times and the average value was taken.

[0108] 1.5.2 Determination of inflammatory factors in serum

[0109] Blood was collected from the eyeballs of mice in each group, and serum was collected. The levels of IL-6 and TNF-α in the serum were detected by ELISA.

[0110] 1.5.3 Masson staining

[0111] Modified Masson trichrome staining solution is an authoritative and classic technical method for collagen fiber staining. It is used to stain collagen fibers. After staining, collagen fibers appear green or blue, while muscle fibers, cytoplasm, keratin, cellulose and red blood cells appear red. Collagen fibers and muscle fibers can be clearly distinguished.

[0112] The mice were fixed on a dissecting board under anesthesia, and fresh skin tissue was obtained from the injured part of the left hind limb, placed in a 1.5 ml enzyme-free Eppendorf tube, and added with 1 ml of 4% paraformaldehyde solution for Masson staining.

[0113] 1.5.4 Oil red staining

[0114] Oil Red O staining is a staining method commonly used to detect fat deposits in tissues. Its main targets are lipid droplets and certain lipoproteins in cells. Triacylglycerols (TAGs) and lipid droplets appear red. Blue or purple represents the cell nucleus. Cytoplasm, cytoplasm, or tissue areas without lipids are usually not stained by Oil Red O, so they remain transparent or show their original color.

[0115] The mice were fixed on a dissecting board under anesthesia, and fresh skin tissue was obtained from the injured part of the left hind limb and placed in a 1.5 ml enzyme-free Eppendorf tube. 1 ml of 4% paraformaldehyde solution was added for oil red staining.

[0116] 1.6 Statistical analysis

[0117] Statistical analysis was performed using Prism 8.0.2, and one-way analysis of variance was used for comparison among groups. The results are expressed as mean ± standard deviation The data were analyzed using t-test. P < 0.05 was considered statistically significant.

[0118] 2 Results

[0119] 2.1 Effect of the Chinese medicine composition of the present invention on the relief of hind limb edema in lymphedema model mice

[0120] result Figure 1 As shown in the figure, during the experiment, there was no significant change in the hind limb circumference of mice in the sham operation group (P>0.05). Compared with the model group, on the 7th and 14th days of administration, the increase in the hind limb circumference of mice in the positive control group, low-dose group, and high-dose group was significantly reduced, with significant differences (P<0.05). In the positive control group, low-dose group, and high-dose group, compared with the first day of administration, the increase in the hind limb circumference of mice in each group on the 7th and 14th days of administration was significantly reduced (P<0.05). Among them, on the 7th and 14th days of administration, the increase in the hind limb circumference of mice in the high-dose group was significantly lower than that in the positive control group.

[0121] 2.2 Effect of the Chinese medicine composition of the present invention on the expression of serum inflammatory factors in lymphedema model mice

[0122] The results are as follows Figure 2 and Figure 3 As shown in the figure, compared with the sham operation group, the relative expression of IL-6 and TNF-α in the serum of the model group mice increased significantly, with significant differences (P<0.05). Compared with the model group, the relative expression of IL-6 and TNF-α in the serum of the positive control group, low-dose group and high-dose group decreased significantly, with significant or extremely significant differences (P<0.05 or P<0.01), among which the relative expression of IL-6 and TNF-α in the serum of the high-dose group mice was significantly lower than that of the positive control group.

[0123] 2.3 Effect of the Chinese medicine composition of the present invention on fibrosis in lymphedema model mice

[0124] The results are as follows Figure 4 As shown, compared with the sham operation group, the model group mice showed obvious fibrosis; compared with the model group, the degree of fibrosis in the positive control group, low-dose group and high-dose group mice was significantly weakened, among which the fibrosis reduction in the high-dose group mice was the largest, and there was no difference compared with the sham operation group.

[0125] 2.4 Effect of the Chinese medicine composition of the present invention on fat deposition in lymphedema model mice

[0126] The results are as follows Figure 5 As shown, compared with the sham operation group, the mice in the model group had obvious fat deposition; compared with the model group, the fat deposition of the mice in the positive control group, low-dose group and high-dose group was significantly reduced, among which the fat deposition of the mice in the high-dose group was most significantly reduced, and there was no difference compared with the sham operation group.

[0127] 3 Conclusion

[0128] The above experimental results show that the Chinese medicine combination of the present invention can significantly reduce the circumference of the hind limbs of mice with lymphedema, has a significant effect of eliminating edema, and effectively reduces the content of inflammatory factors in serum, and has a significant anti-inflammatory effect; at the same time, it can significantly reduce the degree of fibrosis in mice with lymphedema model and improve fat deposition. The Chinese medicine composition of the present invention has a significant therapeutic effect on mice with lymphedema model, and the therapeutic effect is significantly better than that of the Western medicine control.

[0129] Test Example 3

[0130] 1 Materials and methods

[0131] 1.1 General Information

[0132] A total of 68 patients with upper limb lymphedema after breast cancer surgery who were admitted to Zhengzhou Central Hospital from June 2023 to September 2024 were selected as the research subjects and divided into an observation group and a control group according to the random number table method, with 34 cases in each group. Observation group: age 40-65 years old, average (53.01±8.74) years old; course of disease: 1-6 months, average (1.57±0.74) months. Control group: age 38-66 years old, average (50.47±7.09) years old; course of disease: 1-6 months, average (1.49±0.63) months. There was no significant difference in the general data of the two groups of patients (P>0.05), and they were comparable.

[0133] 1.2 Diagnostic criteria

[0134] The diagnostic criteria for upper limb lymphedema after breast cancer surgery were established according to Breast Oncology (2013 Edition), i.e., edema of the affected limb occurs after surgery and the difference in arm circumference between the affected side and the healthy side is >2cm.

[0135] 1.3 Inclusion criteria

[0136] Meet the above-mentioned Western medicine diagnostic criteria; unilateral breast cancer presents with edema in one upper limb; upper limb edema is caused by obstructed lymphatic drainage; good compliance and normal mental state; the patient or family member is informed and signs the informed consent form.

[0137] 1.4 Exclusion criteria

[0138] Allergic to the study drugs; upper limb swelling caused by other reasons; hypoproteinemia; combined with serious primary medical diseases; local infection before enrollment; patients with tumor metastasis; combined with deep vein thrombosis; combined with abnormal function of important organs such as heart, lung, kidney, etc.; acute lymphangitis.

[0139] 1.5 Methods

[0140] Control group: given conventional comprehensive treatment, including limb function training, elastic sleeve, air wave pressure circulation and centripetal massage before bedtime; limb function training was performed once a day in the morning and afternoon, 30 min / time; air wave pressure circulation was performed once a day, 30 min / time; centripetal massage before bedtime was performed from the wrist of the affected limb to the center of the affected limb using kneading, pinching, holding and other techniques, 20 min / time.

[0141] Observation group: on the basis of the above treatment, the patients were given a decoction of the Chinese medicine composition of the present invention; the specific composition was: 30 parts of raw astragalus, 15 parts of Perennial Perilla Cortex, 20 parts of raw Atractylodes macrocephala, 10 parts of Angelica sinensis, 10 parts of Big Belly Cortex, 12 parts of Peach Kernel, 12 parts of Carthamus tinctorius, 12 parts of Ligusticum chuanxiong, 10 parts of Red Peony Root, 6 parts of Pheretima, 30 parts of Millettia Spatholobi, 15 parts of Gleditsia sinensis Spinosa, 15 parts of Fructus Ligulariae, 3 parts of Hirudo, 12 parts of Costusroot, and 10 parts of Toosendan Fructus; decocted in water, 1 dose per day, taken twice a day, morning and evening.

[0142] The above treatments were continued for 2 weeks.

[0143] 1.6 Observation indicators

[0144] 1.6.1 Swelling circumference

[0145] Before and after treatment, the circumference of the wrist crease, 5 cm above the wrist crease, 10 cm below the elbow crease, 10 cm above the elbow crease, and the axilla were measured with a tape measure.

[0146] 1.6.2 Pain level

[0147] Before and after treatment, the visual analogue scale was used to measure the pain level of the affected limb of the patient, with a score of 0-10. The higher the score, the more obvious the pain.

[0148] 1.6.3 Extent of limb swelling

[0149] Limb circumference ratio and tissue water ratio were tested before and after treatment. Multi-frequency bioimpedance human tissue composition analyzer was used to detect the degree of tissue edema of the healthy upper limb and the affected upper limb, that is, the extracellular fluid content. The difference in water (circumference) percentage = affected limb water (circumference) value - healthy side water (circumference) value / healthy limb water (circumference) value.

[0150] 1.6.4 Criteria for determining efficacy

[0151] Markedly effective: After treatment, the swelling of the affected limb completely subsided, and the maximum difference between the measured values ​​at each point was <2cm; Effective: After treatment, the affected limb had mild lymphedema, and the maximum difference between the measured values ​​at each point was 2-5cm; Ineffective: After treatment, the swelling did not change or worsened.

[0152] 1.7 Statistical methods

[0153] SPSS 21.0 statistical software was used to express measurement data as mean ± standard deviation, non-independent sample t test was used between groups, paired sample t test was used within groups, count data were expressed as percentages, and inter-group comparisons were performed using the χ 2 Test, P < 0.05 was considered statistically significant.

[0154] 2 Results

[0155] 2.1 Comparison of clinical efficacy between the two groups of patients

[0156] The results are shown in Table 1. The total effective rate of the observation group was 97.06%, significantly higher than 70.59% of the control group.

[0157] Table 3 Comparison of clinical efficacy between the two groups of patients [n (%)]

[0158] Group Number of cases Significant effect efficient invalid Total Effectiveness Observation Group 34 21(61.76) 12(35.29) 1(2.94) 33(97.06) Control group 34 10(29.41) 14(41.18) 10(29.41) 24(70.59)

[0159] 2.2 Comparison of swelling and pain levels between the two groups before and after treatment

[0160] There was no significant difference in the peripheral diameter ratio, tissue water ratio and pain severity score between the two groups before treatment (P>0.05). After treatment, the peripheral diameter ratio, tissue water ratio and pain severity score of the two groups were significantly lower than those before treatment, with significant differences (P<0.05); at the same time, the peripheral diameter ratio, tissue water ratio and pain severity score of the observation group were significantly lower than those of the control group, with significant differences (P<0.05).

[0161] Table 4 Comparison of swelling and pain levels between the two groups before and after treatment

[0162]

[0163] Note: Compared with the group before treatment, *P<0.05; compared with the control group after treatment, # P<0.05.

[0164] 3 Conclusion

[0165] The Chinese medicine composition of the present invention can significantly improve the total effective rate of treating lymphedema, significantly reduce the degree of limb swelling of patients, and reduce the pain of patients. The Chinese medicine composition of the present invention can be used to prepare a new drug for treating lymphedema.

[0166] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for treating lymphedema, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 20-40 parts of raw astragalus, 5-25 parts of perilla bark, 10-30 parts of raw atractylodes, 5-20 parts of angelica, 5-20 parts of tangerine peel, 5-20 parts of peach kernel, 5-20 parts of safflower, 5-20 parts of ligusticum chuanxiong, 5-20 parts of red peony root, 1-15 parts of earthworm, 20-40 parts of millettia reticulata, 5-20 parts of gleditsia thorn, 5-20 parts of rhizoma liquidambar formosana, 1-15 parts of leech, 5-20 parts of costusroot, and 5-20 parts of toosendan fruit.

2. A Chinese medicine composition for treating lymphedema according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 25-35 parts of raw astragalus, 10-20 parts of cortex rotundifolia, 15-25 parts of raw atractylodes, 5-15 parts of angelica, 5-15 parts of tangerine peel, 10-20 parts of peach kernel, 10-20 parts of safflower, 10-20 parts of ligusticum chuanxiong, 5-15 parts of red peony root, 5-15 parts of earthworm, 25-35 parts of millettia reticulata, 10-20 parts of thorns of gleditsia sinensis, 10-20 parts of rhizoma liquidambar formosana, 1-10 parts of leech, 10-20 parts of costusroot, and 5-15 parts of toosendan fruit.

3. A Chinese medicine composition for treating lymphedema according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of raw astragalus, 15 parts of cortex rotundus, 20 parts of raw atractylodes, 10 parts of angelica sinensis, 10 parts of tangerine peel, 12 parts of peach kernel, 12 parts of safflower, 12 parts of ligusticum chuanxiong, 10 parts of red peony root, 6 parts of earthworm, 30 parts of millettia reticulata, 15 parts of gleditsia thorn, 15 parts of rhizoma liquidambar formosana, 3 parts of leech, 12 parts of costusroot, and 10 parts of toosendan fruit.

4. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicament for treating or improving lymphedema, characterized in that: The Chinese medicine composition is prepared into decoction, pills, oral liquid, granules, powder or other pharmaceutically acceptable oral dosage forms by conventional pharmaceutical preparation methods and with medically acceptable auxiliary materials.

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