Composition for treating myocardial cell hypoxia and preparation method thereof

Abstract

The invention provides a composition for treating myocardial cell hypoxia and a preparation method thereof, and relates to the field of traditional Chinese medicinal materials. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5 to 10 parts of radix rehmanniae, 5 to 15 parts of caulis spatholobi, 5 to 10 parts of radix ophiopogonis, 5 to 7 parts of liquorice root, 3 to 7 parts of radix polygoni multiflori, 2 to 5 parts of colla corii asini, 2 to 5 parts of fructus schisandrae chinensis, 2 to 5 parts of radix codonopsis, 1 to 5 parts of tortoise shell processed with vinegar, 5 to 8 parts of fructus ziziphi jujubae and 3 to 8 parts of ramulus cinnamomi. The preparation method comprises the following steps: adding water into the vinegar-processed tortoise shells, heating and decocting for 2-3 hours, then adding the caulis spatholobi, the codonopsis pilosula and the Chinese dates, decocting for 1-2 hours, filtering, and concentrating filtrate for later use; adding colla corii asini, heating, and uniformly stirring to obtain a mixture; the preparation method comprises the following steps: crushing rehmannia, radix ophiopogonis, liquorice, polygonum multiflorum, schisandra chinensis and cassia twig, soaking in an alcohol aqueous solution, percolating for 20-24 hours, and collecting percolate; and combining the mixture with the percolate, and concentrating under reduced pressure. Through the action mechanism of tonifying qi, nourishing yin, promoting coronary circulation and relieving pain, the treatment effect is jointly achieved. The traditional Chinese medicine composition can be used for treating myocardial cell ischemia caused by coronary heart disease, angina pectoris, arrhythmia and the like.

Description

Technical Field

[0001] The present invention relates to the field of Chinese medicinal materials, and in particular to a composition for treating myocardial cell hypoxia and a preparation method thereof. Background Art

[0002] Myocardial ischemia is a common pathological condition of the heart, mainly caused by coronary heart disease, thrombosis, vasospasm, etc., which causes a decrease in cardiac blood perfusion, leading to myocardial hypoxia and abnormal energy metabolism. Symptoms of myocardial ischemia include chest pain, palpitations, shortness of breath, etc., and in severe cases can lead to myocardial infarction. In addition, there are also symptoms such as shoulder pain, stomach pain, anxiety and insomnia, increased heartbeat, excessive sweating, shortness of breath, dizziness and extreme fatigue.

[0003] In the field of Western medicine, the following drugs are mainly used to treat myocardial ischemia: Nitrates, which relieve myocardial ischemia by dilating coronary arteries and veins, increasing myocardial blood supply, reducing cardiac preload and afterload, and reducing myocardial oxygen consumption. Calcium ion antagonists, which inhibit calcium ions from entering myocardial cells, relax vascular smooth muscle, dilate coronary arteries, increase myocardial blood supply, and reduce myocardial oxygen consumption. Angiotensin converting enzyme inhibitors, which inhibit angiotensin converting enzyme, reduce the production of angiotensin II, dilate peripheral blood vessels, reduce cardiac preload and afterload, and improve ventricular remodeling, thereby treating myocardial ischemia. Nicorandil, which dilates coronary arteries, protects myocardial mitochondria, and reduces ischemia / reperfusion injury through the dual effects of opening ATP-sensitive potassium channels and activating guanylate cyclase.

[0004] In the field of traditional Chinese medicine, angelica has the effects of nourishing blood, promoting blood circulation, regulating menstruation and relieving pain, reducing platelet aggregation, resisting thrombosis, increasing coronary blood flow, reducing myocardial oxygen consumption, and can be used to combat myocardial ischemia; Astragalus has a cardiotonic effect, can dilate coronary arteries, increase myocardial blood flow, improve the body's antioxidant capacity, and reduce the damage of oxygen free radicals to the myocardium; Safflower has a cardiotonic effect, can reduce myocardial oxygen consumption, reduce the scope of myocardial infarction, inhibit platelet aggregation, dilate blood vessels, and reduce peripheral vascular resistance; Salvia miltiorrhiza has the effects of promoting blood circulation and removing blood stasis, removing blood stasis and unblocking collaterals, can improve myocardial blood circulation, and alleviate the symptoms of myocardial ischemia; in addition, Chinese medicines such as Panax notoginseng, Chuanxiong, Pueraria lobata, and Aucklandia lappa can also be used to improve myocardial ischemia. There are many Chinese medicinal materials with the effects of promoting blood circulation and nourishing blood, but there is a lack of a combination with good therapeutic effects. Summary of the invention

[0005] The object of the present invention is to provide a composition for treating myocardial cell hypoxia, which has an excellent therapeutic effect on myocardial cell hypoxia.

[0006] Another object of the present invention is to provide a method for preparing a composition for treating myocardial cell hypoxia, which method can fully extract the effective ingredients in the raw materials and has high utilization efficiency of the raw materials.

[0007] The present invention solves the technical problem by adopting the following technical solutions.

[0008] In one aspect, an embodiment of the present invention provides a composition for treating myocardial cell hypoxia, comprising the following raw materials in parts by weight:

[0009] 5-10 parts of Rehmannia root, 5-15 parts of Millettia reticulata, 5-10 parts of Ophiopogon japonicus, 5-7 parts of Licorice root, 3-7 parts of Polygonum multiflorum, 2-5 parts of donkey-hide gelatin, 2-5 parts of Schisandra chinensis, 2-5 parts of Codonopsis pilosula, 1-5 parts of tortoise shell processed with vinegar, 5-8 parts of jujube, and 3-8 parts of cassia twig.

[0010] In some embodiments of the present invention, the ingredients include, by weight: 10 parts of Rehmannia root, 5 parts of Millettia reticulata, 10 parts of Ophiopogon japonicus, 7 parts of Licorice root, 3 parts of Polygonum multiflorum, 3 parts of donkey-hide gelatin, 3 parts of Schisandra chinensis, 3 parts of Codonopsis pilosula, 5 parts of tortoise shell processed with vinegar, 5 parts of jujube, and 5 parts of cassia twig.

[0011] In some embodiments of the present invention, the ingredients include, by weight: 10 parts of Rehmannia root, 15 parts of Millettia spatholobi, 5 parts of Ophiopogon japonicus, 5 parts of Licorice root, 7 parts of Polygonum multiflorum, 5 parts of donkey-hide gelatin, 5 parts of Schisandra chinensis, 5 parts of Codonopsis pilosula, 1 part of tortoise shell processed with vinegar, 8 parts of jujube, and 3 parts of cassia twig.

[0012] Rehmannia root: sweet, bitter, and cold in nature. It enters the heart, liver, and kidney meridians. It is sweeter than bitter, moist and nourishing, bitter and cold, and clears away heat; it enters the kidney meridian, nourishes yin and produces body fluid, lubricates the large intestine, quenches thirst, and relieves constipation. It has the effects of clearing heat and producing body fluid, cooling blood and stopping bleeding, clearing heat and nourishing yin, clearing heat and cooling blood, and eliminating restlessness and stopping bleeding. It can be used to treat symptoms such as febrile diseases that damage yin and body fluids and constipation; Rehmannia root can remove evil and strengthen the body, and can be used for blood heat, yin deficiency with heat, yin blood deficiency, and dry body fluids and intestines. It is most suitable for those with excessive heat and yin damage. It mainly contains cyclic terpene glycosides such as catalpol, dihydroxycatalpol, acetyl catalpol, rehmannia glycoside, aucubin, milite glycoside, monomilite glycoside, didanosyl gardenia glycoside, and spondylus glycoside, as well as phenylethanoid glycosides such as verbascoside. In addition, it also contains β-sitosterol, various amino acids, and sugars.

[0013] Millettia reticulata: It has the effects of nourishing blood and yin, promoting blood circulation and unclogging meridians, and is often used to treat diseases related to blood deficiency and blood stasis; it has multiple pharmacological effects such as dilating blood vessels, anti-platelet aggregation, lowering blood lipids, improving atherosclerosis, promoting hematopoiesis, analgesia, anti-tumor, and antiviral.

[0014] Ophiopogon japonicus: Ophiopogon japonicus is sweet, slightly bitter, and slightly cold in nature. It enters the heart, lung, and stomach meridians. Ophiopogon japonicus is moist and sweet, slightly bitter and slightly cold, and clears and drains. It enters the lung, heart, and stomach meridians, and is a nourishing and refreshing product. It nourishes yin and blood, and is often used to nourish yin and clear heat, and treat symptoms such as yin deficiency and internal heat; it nourishes yin and produces fluid, moistens the lungs and benefits the stomach, and clears and nourishes the mind to relieve restlessness and calm the nerves, and also moisturizes the intestines and relieves constipation, and treats lung and stomach yin deficiency, restlessness and insomnia, and dry intestines and constipation. The main ingredients are saponins and high isoflavones, as well as a variety of amino acids, trace elements, vitamin A-like substances, polysaccharides and other ingredients.

[0015] Licorice: The medicinal properties are sweet and neutral, and it enters the heart, lung, spleen, and stomach meridians. Licorice is sweet, nourishing, and moistening. It is cool when raw, and warm when roasted. It mainly enters the spleen and lung meridians, and also enters the stomach and heart meridians. It has the effects of invigorating qi, relieving urgency and nourishing the heart. It is often used to reconcile the medicinal properties and enhance the effect of prescriptions. It can not only invigorate qi and replenish the middle, but also relieve urgency and pain, moderate the medicinal properties, and also eliminate phlegm and cough, and detoxify. Honey roasting has strong effects of invigorating qi and relieving urgency; raw use can purge fire and detoxify. It contains triterpenoid saponins and flavonoids.

[0016] Polygonum multiflorum: bitter, sweet, astringent, slightly warm in nature. Enters the liver, heart, and kidney meridians. Polygonum multiflorum enters the liver and kidney meridians, and has different properties and effects when used raw or processed. When used in processed form, it is slightly warm, sweet and astringent, not greasy or dry, good at nourishing the liver and kidney, benefiting the essence and blood, and blackening the hair. It is a good tonic medicine and is good at treating the deficiency of essence and blood. When used raw, it is flat and slightly cool, more bitter than sweet, good at purging but weak in tonifying deficiency, can detoxify, stop malaria, moisten the intestines, and treat sores, scrofula, long-term malaria due to physical weakness, and constipation due to dry intestines. It contains anthraquinone compounds, the main components of which are chrysophanol and rhubarb, as well as lecithin, crude fat, etc. It has the functions of detoxification, eliminating carbuncle, stopping malaria, and moistening the intestines.

[0017] Donkey-hide gelatin is a solid gelatin made from the dried or fresh skin of the donkey, an equine animal, through decoction and concentration. It has the effects of nourishing blood, stopping bleeding, nourishing yin and moistening dryness, and is often used for blood deficiency and sallow complexion, dizziness and palpitations, muscle weakness and weakness; various bleeding syndromes; Yin deficiency syndrome and dryness syndrome. Donkey-hide gelatin is sweet in taste and neutral in nature. It enters the lung, liver and kidney meridians. It mainly contains protein and peptide components, and after hydrolysis, it can obtain a variety of amino acids, such as glycine, glutamic acid, alanine, arginine, aspartic acid, lysine, phenylalanine, serine, histidine, etc. Donkey-hide gelatin fried with clam powder is good at nourishing the lungs and moistening dryness, and is used for Yin deficiency cough, long-term cough with little sputum or blood in sputum. Donkey-hide gelatin fried with pollen has a hemostatic effect, and is mostly used for Yin deficiency and hemoptysis, metrorrhagia, and bloody stool. It nourishes yin and blood, and is often used to nourish blood and stop bleeding, and treat symptoms such as blood deficiency and sallow complexion;

[0018] Schisandra chinensis: The medicinal properties are sour, sweet, and warm. It enters the lung, heart, and kidney meridians. Schisandra chinensis is sour, astringent, moist, warm, and tonic. It has both astringent and tonic properties and enters the lung, kidney, and heart meridians. It can astringe the lungs, relieve cough and asthma, nourish the kidneys, astringe the essence, and stop diarrhea. It can produce fluid, calm the mind, and soothe the nerves. It can also consolidate the exterior, astringe, and stop sweating. It has a strong medicinal effect and is an important medicine for tonifying, strengthening, and astringing. It contains lignans, volatile oils, polysaccharides, amino acids, and other ingredients. It has the functions of astringing, strengthening, replenishing qi, producing fluid, and tonifying the kidney and calming the mind. It is often used to treat symptoms such as palpitations and insomnia.

[0019] Codonopsis: Sweet and mild in nature. It enters the spleen and lung meridians. Codonopsis is sweet and mild, not dry or greasy, and enters the spleen and lung meridians. Its ability to replenish qi is inferior to that of ginseng, and it is mostly used for mild symptoms of spleen and lung qi deficiency. It also produces body fluid and nourishes blood, and can treat symptoms such as body fluid deficiency and blood deficiency. It mainly contains codonopsis glycosides, glucose, inulin, polysaccharides, codonopsis alkaloids, volatile oils, flavonoids, phytosterols, trace elements and other ingredients. It has the effects of replenishing qi and blood, nourishing the heart, and is often used to enhance human immunity and improve symptoms of qi deficiency;

[0020] Vinegar-processed tortoise shell: slightly cold in nature, sweet and salty in taste, and enters the liver, kidney, and heart meridians. It nourishes blood and yin, suppresses yang and extinguishes wind, and is often used to treat symptoms such as yin deficiency and yang hyperactivity; its main effects include nourishing yin and suppressing yang, strengthening the kidney and bones, nourishing blood and nourishing the heart, consolidating the meridians and stopping bleeding, etc. After being processed with vinegar, the tortoise shell becomes crisp and easy to crush, which is conducive to decocting the effective ingredients and can correct the odor and taste. In clinical practice, vinegar-processed tortoise shell is often used to treat symptoms such as kidney yin deficiency, bone steaming and hot flashes, night sweats, spermatorrhea, metrorrhagia and leukorrhea, as well as symptoms such as irritability, insomnia, vomiting blood, and epistaxis. In addition, vinegar-processed tortoise shell can also be used to nourish the heart and treat symptoms such as palpitations, insomnia, and forgetfulness.

[0021] Jujube: It has the functions of replenishing Qi and blood, nourishing the heart and calming the mind, and is often used to improve symptoms such as blood deficiency and insomnia; it is rich in glucose, fructose, vitamin C, various amino acids and minerals. It is a natural source of energy and helps to enhance physical strength and immunity.

[0022] Cinnamon twig: pungent, sweet, warm. It enters the heart, lung, and bladder meridians. Cinnamon twig is pungent, dispersing, warming, and tonifying. It is sweet, warm, and yang-boosting. It warms and tonifies, enters the lung and bladder meridians, and is good at dispersing wind-cold and relieving wind-cold or wind evil on the surface. It enters the heart meridian and blood, and is good at warming, tonifying, and tonifying, dispersing cold evil, unblocking blood vessels, unblocking chest yang, warming and transforming dampness, and relieving pain. It contains volatile oils, phenols, organic acids, polysaccharides, glycosides, coumarins, and tannins, and has the effects of sweating, relieving muscles, warming and unblocking meridians, assisting yang and transforming qi, and calming and descending qi. It is often used to treat symptoms such as pain caused by cold stagnation and blood stasis.

[0023] The raw materials in the present invention are used in combination to jointly exert therapeutic effects through the mechanism of invigorating qi and nourishing yin, unblocking meridians and relieving pain. Among them, Rehmannia root, Radix Ophiopogonis and the like nourish yin and blood, Millettia spatholobi and Carapace of Tortoise promote blood circulation and unblock meridians, and Cinnamon twig warms yang and unblocks meridians, and can be used to treat myocardial cell ischemia caused by coronary heart disease, angina pectoris and arrhythmia.

[0024] In some embodiments of the present invention, the tortoise shell prepared with vinegar is prepared by the following method: adding stone sand to a pot, adding it to 100°C, then adding raw tortoise shell, Codonopsis pilosula and Angelica sinensis, stir-frying until the tortoise shell is light yellow, taking out the tortoise shell, Codonopsis pilosula and Angelica sinensis, placing them in vinegar, soaking them for 1-2 hours, taking out the tortoise shell, drying it, and crushing it. When preparing the tortoise shell prepared with vinegar, Codonopsis pilosula and Angelica sinensis are added and prepared at the same time. During the frying process, the moisture in Codonopsis pilosula and Angelica sinensis can be removed, and then they are soaked in vinegar together with the tortoise shell, and their effective ingredients can be released and then soaked in the tortoise shell, enriching the efficacy of the tortoise shell.

[0025] An embodiment of the present invention provides a method for preparing a composition for treating myocardial cell hypoxia, comprising the following steps:

[0026] S1, adding water to the vinegar-processed tortoise shell, heating to 100-110° C., boiling for 2-3 hours, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 100-110° C. for 1-2 hours, filtering, concentrating the filtrate, and setting aside;

[0027] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 50-60° C., and stirring evenly to obtain a mixture;

[0028] S3, crushing Rehmannia root, Radix Ophiopogonis, Glycyrrhiza uralensis, Polygonum multiflorum, Fructus Schisandrae Chinensis and Cinnamomum cassia, and then placing them in an alcohol aqueous solution, soaking for 1-2 hours, adding them to a percolation cylinder, using alcohol as a solvent, percolating for 20-24 hours, and collecting the percolation liquid;

[0029] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure.

[0030] In some embodiments of the present invention, gelatin is further added to the concentrated solution of step S4 to prepare tablets or pills.

[0031] In some embodiments of the present invention, the mass ratio of the gelatin to the concentrated solution is 1:(1-2).

[0032] Compared with the prior art, the embodiments of the present invention have at least the following advantages or beneficial effects:

[0033] The raw materials in the present invention are used in combination to jointly exert therapeutic effects through the mechanism of invigorating qi and nourishing yin, unblocking meridians and relieving pain. Among them, Rehmannia root, Radix Ophiopogonis and the like nourish yin and blood, Millettia spatholobi and Carapace of Tortoise promote blood circulation and unblock meridians, and Cinnamon twig warms yang and unblocks meridians, and can be used to treat myocardial cell ischemia caused by coronary heart disease, angina pectoris and arrhythmia.

[0034] When preparing the composition of the present invention, firstly, raw materials such as tortoise shell made with vinegar, Millettia reticulata, Codonopsis pilosula, and jujube are decocted so that the effective ingredients therein are fully dissolved in water, and in the process of decocting, the effective ingredients interact with each other to provide the efficacy of the composition; then, donkey-hide gelatin is added to absorb the effective ingredients, and effective ingredients such as Rehmannia root, Ophiopogon japonicus, Licorice, Polygonum multiflorum, Schisandra chinensis, and Cinnamomum cassia are extracted by osmosis, and finally the extracted effective ingredients are combined and concentrated. In the present invention, different extraction methods are adopted for different drugs, so that the effective ingredients in the raw materials can be fully utilized, the loss of the effective ingredients can be reduced, and the content of the effective ingredients in the composition can be provided. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0036] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other. The present invention will be described in detail below with reference to specific embodiments.

[0037] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0038] Example 1

[0039] Prepare the raw materials according to the following ratio:

[0040] 10 parts of Rehmannia root, 5 parts of Millettia reticulata, 10 parts of Ophiopogon japonicus, 7 parts of Licorice, 3 parts of Polygonum multiflorum, 3 parts of donkey-hide gelatin, 3 parts of Schisandra chinensis, 3 parts of Codonopsis pilosula, 5 parts of tortoise shell processed with vinegar, 5 parts of jujube, and 5 parts of cassia

[0041] Prepare the pills as follows:

[0042] S1, adding water to the vinegar-processed tortoise shell, heating to 100±5°C, and boiling for 3h, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 100±5°C for 2h, filtering, concentrating the filtrate, and setting aside;

[0043] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 50±2° C., and stirring evenly to obtain a mixture;

[0044] S3, crushing Rehmannia root, Ophiopogon japonicus, Licorice root, Polygonum multiflorum, Schisandra chinensis and Cinnamon twig into particles with a particle size of 0.5-0.8 cm, and then placing them in an alcohol aqueous solution (80% by volume), soaking for 2 hours, adding them to a percolation cylinder, using alcohol (80% by volume) as a solvent, percolating for 20 hours, and collecting the percolation liquid;

[0045] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure until the liquid volume is reduced by 1 / 3.

[0046] S5, adding gelatin to the concentrated solution of step S4 to prepare pills, wherein the mass ratio of the added gelatin to the concentrated solution is 1:1.

[0047] Example 2

[0048] Prepare the raw materials according to the following ratio:

[0049] 10 parts of Rehmannia root, 15 parts of Millettia spatholobi, 5 parts of Ophiopogon japonicus, 3 parts of Licorice, 7 parts of Polygonum multiflorum, 5 parts of donkey-hide gelatin, 5 parts of Schisandra chinensis, 5 parts of Codonopsis pilosula, 1 part of tortoise shell processed with vinegar, 8 parts of jujube, and 3 parts of cassia

[0050] Prepare the pills as follows:

[0051] S1, adding water to the vinegar-processed tortoise shell, heating to 100±5°C, and boiling for 3h, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 100±5°C for 2h, filtering, concentrating the filtrate, and setting aside;

[0052] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 50±2° C., and stirring evenly to obtain a mixture;

[0053] S3, crushing Rehmannia root, Ophiopogon japonicus, Licorice root, Polygonum multiflorum, Schisandra chinensis and Cinnamon twig into particles with a particle size of 0.5-0.8 cm, and then placing them in an alcohol aqueous solution (80% by volume), soaking for 2 hours, adding them to a percolation cylinder, using alcohol (80% by volume) as a solvent, percolating for 20 hours, and collecting the percolation liquid;

[0054] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure until the liquid volume is reduced by 1 / 3.

[0055] S5, adding gelatin to the concentrated solution of step S4 to prepare pills, wherein the mass ratio of the added gelatin to the concentrated solution is 1:1.

[0056] Example 3

[0057] Prepare the raw materials according to the following ratio:

[0058] 5 parts of Rehmannia root, 10 parts of Millettia spatholobi, 8 parts of Ophiopogon japonicus, 5 parts of Licorice root, 3 parts of Polygonum multiflorum, 2 parts of donkey-hide gelatin, 52 parts of Schisandra chinensis, 2 parts of Codonopsis pilosula, 5 parts of tortoise shell processed with vinegar, 5 parts of jujube, and 8 parts of cas

[0059] Prepare the pills as follows:

[0060] S1, adding water to the vinegar-processed tortoise shell, heating to 110±5°C, boiling for 2h, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 110±5°C for 1h, filtering, concentrating the filtrate, and setting aside;

[0061] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 60±2° C., and stirring evenly to obtain a mixture;

[0062] S3, crushing Rehmannia root, Ophiopogon japonicus, Licorice root, Polygonum multiflorum, Schisandra chinensis and Cinnamon twig into particles with a particle size of 0.8-1 cm, then placing them in an alcohol aqueous solution (80% by volume), soaking for 2 hours, adding them to a percolation cylinder, using alcohol (80% by volume) as a solvent, percolating for 24 hours, and collecting the percolation liquid;

[0063] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure until the liquid volume is reduced by 1 / 3.

[0064] S5, adding gelatin to the concentrated solution of step S4 to prepare pills, wherein the mass ratio of the added gelatin to the concentrated solution is 1:1.

[0065] Example 4

[0066] Prepare the raw materials according to the following ratio:

[0067] 5 parts of Rehmannia root, 15 parts of Millettia reticulata, 10 parts of Ophiopogon japonicus, 6 parts of Licorice root, 7 parts of Polygonum multiflorum, 5 parts of donkey-hide gelatin, 3 parts of Schisandra chinensis, 3 parts of Codonopsis pilosula, 5 parts of tortoise shell processed with vinegar, 8 parts of jujube, and 5 parts of cassia

[0068] Tablets were prepared as follows:

[0069] S1, adding water to the vinegar-processed tortoise shell, heating to 110±5°C, boiling for 3h, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 110±5°C for 2h, filtering, concentrating the filtrate, and setting aside;

[0070] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 60±2° C., and stirring evenly to obtain a mixture;

[0071] S3, crushing Rehmannia root, Ophiopogon japonicus, Licorice root, Polygonum multiflorum, Schisandra chinensis and Cinnamon twig into particles with a particle size of 0.5-0.8 cm, and then placing them in an alcohol aqueous solution (80% by volume), soaking for 2 hours, adding them to a percolation cylinder, using alcohol (80% by volume) as a solvent, percolating for 20 hours, and collecting the percolation liquid;

[0072] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure until the liquid volume is reduced by 1 / 3.

[0073] S5, adding gelatin to the concentrated solution of step S4 to prepare tablets, wherein the mass ratio of the added gelatin to the concentrated solution is 1:2.

[0074] Example 5

[0075] Prepare the raw materials according to the following ratio:

[0076] 8 parts of Rehmannia root, 10 parts of Millettia spatholobi, 8 parts of Ophiopogon japonicus, 5 parts of Licorice root, 7 parts of Polygonum multiflorum, 5 parts of donkey-hide gelatin, 5 parts of Schisandra chinensis, 5 parts of Codonopsis pilosula, 1 part of tortoise shell processed with vinegar, 8 parts of jujube, and 8 parts of cassia twig.

[0077] Tablets were prepared as follows:

[0078] S1, adding water to the vinegar-processed tortoise shell, heating to 100±5°C, boiling for 3h, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 100±5°C for 2h, filtering, concentrating the filtrate, and setting aside;

[0079] S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 50±2° C., and stirring evenly to obtain a mixture;

[0080] S3, crushing Rehmannia root, Ophiopogon japonicus, Licorice root, Polygonum multiflorum, Schisandra chinensis and Cinnamon twig into particles with a particle size of 0.5-0.8 cm, and then placing them in an alcohol aqueous solution (80% by volume), soaking for 2 hours, adding them to a percolation cylinder, using alcohol (80% by volume) as a solvent, percolating for 20 hours, and collecting the percolation liquid;

[0081] S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure until the liquid volume is reduced by 1 / 3.

[0082] S5, adding gelatin to the concentrated solution of step S4 to prepare tablets, wherein the mass ratio of the added gelatin to the concentrated solution is 1:1.5.

[0083] Typical Effects

[0084] 1. Wang, male, 52 years old, suffered from coronary heart disease in 2014, shortness of breath, heart area thorn, chest tightness, arrhythmia, long-term use of Western medicine, can properly relieve symptoms. Follow-up switched to traditional Chinese medicine treatment, taking the pills of Example 1, 3 times a day, 5g at a time. After taking it for 15 days, the chest pain and chest tightness symptoms disappeared, and the medication was continued for 2 months, during which there was no onset of symptoms such as shortness of breath, heart area thorn, chest tightness, chest pain, etc., and the medication was reduced to once a day.

[0085] 2. Liu, female, 35 years old, suffered from sudden chest tightness and dull pain in the chest, and was diagnosed with coronary heart disease, accompanied by arrhythmia. She took the pills of Example 1, 3 times a day, 3 g each time. After taking it for 7 days, the symptoms of chest tightness and dull pain in the chest disappeared. She continued to take the medicine for 1 month and then stopped taking it. During this period, there were no symptoms of chest tightness or dull pain in the chest. During the follow-up of 1 year, there were no symptoms of chest tightness or dull pain in the chest.

[0086] 3. Jiang Moumou, male, 43 years old, suffered from coronary heart disease and diabetes for many years. He suffered from occasional chest tightness, palpitations and shortness of breath during his illness. He took the pills of Example 1, 3 times a day, 5g each time. After taking it for 1 month, the symptoms of chest tightness, palpitations and shortness of breath disappeared. He continued to take it for 2 months, and there were no symptoms such as chest tightness, palpitations and shortness of breath during the period. After stopping the drug for 1 year, there were no symptoms such as chest tightness, palpitations and shortness of breath during the follow-up.

[0087] In summary, the various raw materials in the present invention are used in combination to jointly exert therapeutic effects through the mechanism of invigorating qi and nourishing yin, unblocking meridians and relieving pain. Among them, Rehmannia root, Radix Ophiopogonis and the like nourish yin and blood, Millettia spatholobi and Carapace of Tortoise promote blood circulation and unblock meridians, and Cinnamon twig warms yang and unblocks meridians, and can be used to treat myocardial cell ischemia caused by coronary heart disease, angina pectoris and arrhythmia.

[0088] The embodiments described above are part of the embodiments of the present invention, rather than all of the embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

Claims

1. A composition for treating myocardial cell hypoxia, characterized in that: The following raw materials are included by weight: 5-10 parts of Rehmannia root, 5-15 parts of Millettia reticulata, 5-10 parts of Ophiopogon japonicus, 5-7 parts of Licorice root, 3-7 parts of Polygonum multiflorum, 2-5 parts of donkey-hide gelatin, 2-5 parts of Schisandra chinensis, 2-5 parts of Codonopsis pilosula, 1-5 parts of tortoise shell processed with vinegar, 5-8 parts of jujube, and 3-8 parts of cassia twig.

2. The composition for treating myocardial cell hypoxia according to claim 1, characterized in that: The ingredients include, by weight: 10 parts of Rehmannia root, 5 parts of Millettia reticulata, 10 parts of Ophiopogon japonicus, 7 parts of Licorice root, 3 parts of Polygonum multiflorum, 3 parts of donkey-hide gelatin, 3 parts of Schisandra chinensis, 3 parts of Codonopsis pilosula, 5 parts of tortoise shell processed with vinegar, 5 parts of jujube, and 5 parts of cassia twig.

3. The composition for treating myocardial cell hypoxia according to claim 1, characterized in that: The ingredients include, by weight: 10 parts of Rehmannia root, 15 parts of Millettia spatholobi, 5 parts of Ophiopogon japonicus, 5 parts of Licorice root, 7 parts of Polygonum multiflorum, 5 parts of donkey-hide gelatin, 5 parts of Schisandra chinensis, 5 parts of Codonopsis pilosula, 1 part of tortoise shell processed with vinegar, 8 parts of jujube, and 3 parts of cassia twig.

4. The composition for treating myocardial cell hypoxia according to claim 1, characterized in that: The vinegar-processed tortoise shell is prepared by the following method: Add sand to the pot and heat it to 100℃, then add raw tortoise shell, Codonopsis pilosula and Angelica sinensis, stir-fry until the tortoise shell turns light yellow, take out the tortoise shell, Codonopsis pilosula and Angelica sinensis, put them all in vinegar, soak for 1-2 hours, take out the tortoise shell, dry it and crush it.

5. A method for preparing a composition for treating myocardial cell hypoxia according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1, adding water to the vinegar-processed tortoise shell, heating to 100-110° C., boiling for 2-3 hours, then adding Millettia reticulata, Codonopsis pilosula, and jujube, boiling at 100-110° C. for 1-2 hours, filtering, concentrating the filtrate, and setting aside; S2, adding donkey-hide gelatin to the filtrate of step S1, heating to 50-60° C., and stirring evenly to obtain a mixture; S3, crushing Rehmannia root, Radix Ophiopogonis, Glycyrrhiza uralensis, Polygonum multiflorum, Fructus Schisandrae Chinensis and Cinnamomum cassia, and then placing them in an alcohol aqueous solution, soaking for 1-2 hours, adding them to a percolation cylinder, using alcohol as a solvent, percolating for 20-24 hours, and collecting the percolation liquid; S4, combining the mixture of step S2 and the percolate of step S3, and concentrating under reduced pressure.

6. The method for preparing the composition for treating myocardial cell hypoxia according to claim 5, characterized in that: The method also includes adding gelatin to the concentrated solution of step S4 to prepare tablets or pills.

7. The method for preparing the composition for treating myocardial cell hypoxia according to claim 6, characterized in that: The mass ratio of the gelatin to the concentrated solution is 1:(1-2).

8. The method for preparing the composition for treating myocardial cell hypoxia according to claim 6, characterized in that: In the step S3, the particle size after crushing is 0.5-1 cm.