Preparation method of traditional Chinese medicine composition for reducing blood sugar

Abstract

A preparation method of the traditional Chinese medicine composition comprises the following steps: (1) selecting and cleaning the fresh radix rehmanniae, the fresh radix puerariae, the fresh rhizoma polygonati, the fresh rhizoma dioscoreae, the fresh radix trichosanthis, the fresh radix ophiopogonis, the fresh ginseng, the fresh radix astragali, the silkworm larva and the fresh ginger, cutting the fresh radix rehmanniae, the fresh radix puerariae, the fresh rhizoma polygonati, the fresh rhizoma dioscoreae, the fresh radix trichosanthis, the fresh radix ophiopogonis, the fresh ginseng, the fresh radix astragali and the fresh ginger into blocks while the fresh radix rehmanniae, the fresh radix puerariae, the fresh rhizoma polygonati, the fresh rhizoma dioscoreae, the fresh radix trichosanthis, the fresh radix ophiopogonis, the fresh ginseng, the fresh radix astragali and the fresh ginger are fresh, adding water, breaking walls, and filtering to obtain filtrate; centrifuging to obtain supernate; (2) performing ultrafiltration, concentration, freezing and crushing on a product obtained in the step (1); (3) washing silkworm larva, cutting into sections, freeze-drying, crushing and sieving; and (4) uniformly mixing the materials obtained in the steps (2) and (3), packaging in vacuum, and sterilizing to obtain the finished product of the traditional Chinese medicine composition for reducing blood sugar. The traditional Chinese medicine composition has the effects of tonifying qi and consolidating root, nourishing yin and clearing heat, engendering liquid and quenching thirst, and clearing heat and purging fire, can effectively control blood sugar, is convenient to operate, safe and environment-friendly, can retain active ingredients in the traditional Chinese medicines to the greatest extent, and has huge economic and social benefits.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine, and in particular to a method for preparing a blood sugar-lowering traditional Chinese medicine composition. Background Art

[0002] Diabetes is a key global public health issue. As people's quality of life improves, the global prevalence of diabetes is gradually increasing. At present, the main drugs for the clinical treatment of diabetes are oral Western medicines, but oral hypoglycemic drugs have certain side effects and secondary failure problems, and long-term use of insulin has problems such as weight gain and decreased sensitivity. Diabetes is called "Xiaoke" in traditional Chinese medicine. Traditional Chinese medicine has unique advantages in the treatment and prevention of diabetes and shows good development prospects. However, there are still problems such as unstable efficacy and uneven quality of preparation process. Therefore, it is necessary to develop a new, efficient and stable traditional Chinese medicine.

[0003] Type 2 diabetes mellitus (T2DM) refers to a chronic disease caused by insufficient insulin secretion and / or insulin resistance (the body's sensitivity to insulin is reduced and cannot be effectively used) due to genetic and / or environmental factors, resulting in increased blood sugar levels. It can cause damage to multiple systems and lead to dysfunction of tissues and organs such as the eyes, kidneys, nerves, heart, and blood vessels. Clinical manifestations include thirst, polydipsia, polyphagia, and polyuria, and weight loss. As type 2 diabetes progresses, blood sugar levels will rise and symptoms will become more and more obvious. Some early patients have delayed peak insulin secretion after eating, and the insulin level rises inappropriately 3-5 hours after a meal, causing hypoglycemia, hunger, tremors, cold sweats, and even fainting, which seriously affects people's physical and mental health and quality of life. T2DM is commonly seen in traditional Chinese medicine as a syndrome of qi and yin deficiency caused by dryness and heat damaging yin and yin damaging qi. The treatment principle is to replenish qi and nourish yin. Fresh Chinese medicine is a special type of Chinese medicinal material, which refers to fresh wet medicinal materials that have just been harvested, to distinguish them from dry or raw products that have been processed into decoction pieces. Using fresh herbs to treat diseases is an important feature of traditional Chinese medicine. It has unique efficacy in the clinical treatment of acute, critical, and difficult diseases, and is particularly good at clearing away heat, promoting fluid production, nourishing yin, and moistening dryness, and its efficacy is better than that of dried herbs. So far, there has been no public report on whether fresh herbs can be used to develop a Chinese medicine composition for the treatment of type 2 diabetes. Summary of the invention

[0004] In view of the above situation, in order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a method for preparing a blood sugar-lowering Chinese medicine composition, which can effectively lower blood sugar levels and solve the problem of medication for treating type 2 diabetes.

[0005] The technical solution provided by the present invention is a Chinese medicine composition for reducing blood sugar, which is prepared from the following raw materials in parts by weight: 10-60 parts of fresh rehmannia root, 10-60 parts of fresh kudzu root, 10-60 parts of fresh polygonatum, 10-60 parts of fresh yam, 10-60 parts of fresh trichosanthes, 10-60 parts of fresh ophiopogon, 10-60 parts of fresh ginseng, 10-60 parts of fresh astragalus, 10-100 parts of white silkworm and 2-6 parts of fresh ginger. The preparation method is as follows:

[0006] (1) The fresh Rehmannia root is selected and washed 1 to 3 times, cut into pieces or slices while fresh, and water is added in an amount of 0.5 to 4 times the weight volume of the original drug, where the weight volume refers to the solid in g and the liquid in mL (the same below), and the cell wall is broken for 1 to 10 minutes, filtered, and the filtrate is centrifuged at 2000 to 8000 r / min for 5 to 30 minutes to obtain a first supernatant; then water is added and the cell wall is broken and centrifuged 1 to 4 times according to the above method to obtain a second to fifth supernatant, and the supernatants of each time are combined;

[0007] After selecting, wash the fresh kudzu root 1 to 3 times, cut into sections or slices while fresh, add water 1 to 5 times the weight and volume of the original drug, break the wall for 1 to 20 minutes, filter, and centrifuge the filtrate at 2000 to 8000 r / min for 5 to 30 minutes to obtain the first supernatant; then add water and repeat the above method of breaking the wall and centrifuging 1 to 4 times to obtain the second to fifth supernatants, and combine the supernatants of each time;

[0008] Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0009] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0010] (3) Wash and cut the Bombyx batryticatus medicinal material into sections, freeze-dry, first grind into coarse powder, pass through an 80-100 mesh sieve, and then ultrafine grind and pass through a 150-300 mesh sieve to obtain Bombyx batryticatus ultrafine powder;

[0011] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a finished Chinese medicine composition for lowering blood sugar, which is stored at -20°C for future use; the sterilization method includes but is not limited to ultra-high pressure sterilization, pasteurization, radiation sterilization, gas sterilization, filtration sterilization, etc.

[0012] The application of the blood sugar lowering Chinese medicine composition in the preparation of blood sugar lowering medicine.

[0013] The blood sugar lowering Chinese medicine composition is further added with pharmaceutically acceptable excipients to prepare blood sugar lowering drugs in different Chinese medicine dosage forms.

[0014] The traditional Chinese medicine dosage forms are granules, tablets and capsules.

[0015] The application of the blood sugar lowering Chinese medicine composition in the preparation of medicines for preventing, alleviating and treating abnormal blood sugar.

[0016] The Chinese medicine composition of the present invention plays a synergistic role according to the primary and secondary relationship of monarch, minister, assistant and envoy, has the effects of invigorating qi and strengthening the foundation, nourishing yin and clearing heat, promoting body fluid and quenching thirst, clearing heat and purging fire, is effectively used for treating hyperglycemia of qi and yin deficiency type T2DM, reduces complications, and long-term use of the Chinese medicine composition of the present invention can effectively control blood sugar and keep it within a normal range. At the same time, the drug composition and preparation method with hypoglycemic effect of the present invention have simple process, convenient operation, safety and environmental protection, can retain the active ingredients in the Chinese medicine to the greatest extent, ensure the efficacy of the medicine, have no toxic side effects when taken for a long time, have good practical clinical application value and development and application prospects, and have huge economic and social benefits. DETAILED DESCRIPTION

[0017] The specific implementation modes of the present invention are described in detail below in combination with embodiments and specific situations.

[0018] Example 1

[0019] The invention discloses a method for preparing a Chinese medicine composition for reducing blood sugar. The Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of fresh rehmannia root, 30 parts of fresh kudzu root, 30 parts of fresh polygonatum, 30 parts of fresh yam, 30 parts of fresh trichosanthes root, 30 parts of fresh ophiopogon, 30 parts of fresh ginseng, 30 parts of fresh astragalus, 2 parts of white silkworm and 4 parts of fresh ginger. The preparation method is as follows:

[0020] (1) The fresh Rehmannia root is selected and washed twice, cut into 2-4 mm thick slices while fresh, add 1 times the weight volume of water of the original drug, break the wall for 2.5 minutes, filter, and centrifuge the filtrate at 4000 r / min for 20 minutes to obtain the first supernatant; then add 3 times the amount of water and repeat the above method for breaking the wall and centrifugation twice to obtain the second and third supernatants, and combine the supernatants of each time;

[0021] Fresh kudzu root was selected and washed twice, and cut into 2-4 mm thick slices while fresh, and water twice the weight volume of the original drug was added, and the cell wall was broken for 5 minutes, filtered, and the filtrate was centrifuged at 4000r / min for 20 minutes to obtain the first supernatant; then 3 times the amount of water was added and the cell wall was broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time were combined;

[0022] Fresh kudzu root, fresh polygonatum, fresh yam, fresh trichosanthes root, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger are prepared into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and fresh ginseng is prepared into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0023] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0024] (3) Washing and cutting the Bombyx batryticatus medicinal material, freeze-drying, first grinding into coarse powder, passing through a 100-mesh sieve, and then ultrafine grinding, passing through a 300-mesh sieve, to obtain ultrafine Bombyx batryticatus powder;

[0025] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

[0026] Example 2

[0027] The invention discloses a method for preparing a Chinese medicine composition for reducing blood sugar. The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh rehmannia root, 20 parts of fresh kudzu root, 20 parts of fresh polygonatum, 20 parts of fresh yam, 20 parts of fresh trichosanthes root, 20 parts of fresh ophiopogon, 10 parts of fresh ginseng, 10 parts of fresh astragalus, 1 part of white silkworm and 2 parts of fresh ginger. The preparation method is:

[0028] (1) The fresh Rehmannia root is selected and washed once, and cut into 2-4 mm thick slices while fresh, and water 3 times the weight volume of the original drug is added to break the cell wall for 5 minutes, filtered, and the filtrate is centrifuged at 2000 r / min for 30 minutes to obtain a first supernatant; then water is added and the cell wall is broken and centrifuged again according to the above method to obtain a second supernatant, and the supernatants of each time are combined;

[0029] Fresh kudzu root was selected and washed once, and cut into 2-4 mm thick slices while fresh, and water of 4 times the weight and volume of the original drug was added, and the cell wall was broken for 10 minutes, filtered, and the filtrate was centrifuged at 2000r / min for 30 minutes to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged again according to the above method to obtain the second supernatant, and the supernatants of each time were combined;

[0030] Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0031] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0032] (3) Washing and cutting the Bombyx Batryticatus medicinal material, freeze-drying, first grinding into coarse powder, passing through a 100-mesh sieve, and then ultrafine grinding, passing through a 220-mesh sieve, to obtain Bombyx Batryticatus ultrafine powder;

[0033] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

[0034] Example 3

[0035] The invention discloses a method for preparing a Chinese medicine composition for reducing blood sugar. The Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh rehmannia root, 25 parts of fresh kudzu root, 25 parts of fresh polygonatum, 25 parts of fresh yam, 25 parts of fresh trichosanthes root, 25 parts of fresh ophiopogon japonicus, 15 parts of fresh ginseng, 15 parts of fresh astragalus, 2 parts of white silkworm and 3 parts of fresh ginger. The preparation method is as follows:

[0036] (1) The fresh Rehmannia root is selected and washed once, cut into 2-4 mm thick slices while fresh, and water 4 times the weight volume of the original drug is added to break the wall for 10 minutes, filtered, and the filtrate is centrifuged at 8000 r / min for 20 minutes to obtain a supernatant;

[0037] Fresh kudzu root was selected and washed once, cut into 2-4 mm thick slices while fresh, added with water 5 times the weight volume of the original drug, broken for 20 minutes, filtered, and the filtrate was centrifuged at 8000r / min for 20 minutes to obtain the first supernatant;

[0038] Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0039] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0040] (3) Washing and cutting the Bombyx Batryticatus medicinal material, freeze-drying, first grinding into coarse powder, passing through an 80-mesh sieve, and then ultrafine grinding, passing through a 150-mesh sieve, to obtain Bombyx Batryticatus ultrafine powder;

[0041] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

[0042] Example 4

[0043] The invention discloses a method for preparing a Chinese medicine composition for reducing blood sugar. The Chinese medicine composition is composed of the following raw materials in parts by weight: 60 parts of fresh rehmannia root, 60 parts of fresh kudzu root, 60 parts of fresh polygonatum, 60 parts of fresh yam, 30 parts of fresh trichosanthes root, 30 parts of fresh ophiopogon, 60 parts of fresh ginseng, 60 parts of fresh astragalus, 5 parts of white silkworm and 6 parts of fresh ginger. The preparation method is as follows:

[0044] (1) The fresh Rehmannia root is selected and washed twice, cut into pieces while fresh, and added with water of 1 times the weight volume of the original drug, broken for 2 minutes, filtered, and the filtrate is centrifuged at 6000r / min for 15 minutes to obtain the first supernatant; then 2 times the amount of water is added and the wall is broken and centrifuged three times according to the above method to obtain the second to fourth supernatants, and the supernatants of each time are combined;

[0045] Fresh kudzu root was selected and washed twice, cut into sections while fresh, added with water twice the weight volume of the original drug, broken for 3 minutes, filtered, and the filtrate was centrifuged at 6000r / min for 15 minutes to obtain the first supernatant; then 2 times the amount of water was added and the wall was broken and centrifuged three times according to the above method to obtain the second to fourth supernatants, and the supernatants of each time were combined;

[0046] Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0047] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0048] (3) Washing and cutting the Bombyx Batryticatus medicinal material, freeze-drying, first grinding into coarse powder, passing through an 80-mesh sieve, and then ultrafine grinding, passing through a 150-mesh sieve, to obtain Bombyx Batryticatus ultrafine powder;

[0049] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

[0050] Example 5

[0051] The invention discloses a method for preparing a Chinese medicine composition for reducing blood sugar. The Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of fresh rehmannia root, 40 parts of fresh kudzu root, 40 parts of fresh polygonatum, 40 parts of fresh yam, 10 parts of fresh trichosanthes root, 10 parts of fresh ophiopogon japonicus, 20 parts of fresh ginseng, 20 parts of fresh astragalus, 3 parts of white silkworm and 5 parts of fresh ginger. The preparation method is as follows:

[0052] (1) The fresh Rehmannia root is selected and washed once, cut into pieces while fresh, and 0.5 times the weight volume of water of the original drug is added, the cell wall is broken for 1 minute, filtered, and the filtrate is centrifuged at 8000r / min for 5 minutes to obtain the first supernatant; then 2 times the amount of water is added and the cell wall is broken and centrifuged again according to the above method for 4 times to obtain the second to fifth supernatants, and the supernatants of each time are combined;

[0053] Fresh kudzu root was selected and washed once, cut into sections while fresh, added with water of 1 times the weight of the original drug, broken for 1 minute, filtered, and the filtrate was centrifuged at 8000r / min for 5 minutes to obtain the first supernatant; then 2 times the amount of water was added and the wall was broken and centrifuged again according to the above method for 4 times to obtain the second to fifth supernatants, and the supernatants of each time were combined;

[0054] Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant;

[0055] (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder;

[0056] (3) Washing and cutting the Bombyx Batryticatus medicinal material, freeze-drying, first grinding into coarse powder, passing through an 80-mesh sieve, and then ultrafine grinding, passing through a 180-mesh sieve, to obtain Bombyx Batryticatus ultrafine powder;

[0057] (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

[0058] The application of the blood sugar lowering Chinese medicine composition in the preparation of blood sugar lowering medicine.

[0059] The blood sugar lowering Chinese medicine composition is further added with pharmaceutically acceptable excipients to prepare blood sugar lowering drugs in different Chinese medicine dosage forms.

[0060] The different traditional Chinese medicine dosage forms are granules, tablets and capsules.

[0061] The application of the blood sugar lowering Chinese medicine composition in the preparation of medicines for preventing, alleviating and treating abnormal blood sugar.

[0062] In the above-mentioned Chinese medicine composition, wherein:

[0063] Fresh Rehmannia root: sweet in taste, cold in nature. Enters the liver and kidney meridians. Nourishes yin and blood, clears heat and cools blood.

[0064] Fresh Pueraria root: sweet, spicy, cool in nature. Enters the spleen, stomach, and lung meridians. Relieves muscle pain, reduces fever, promotes the production of body fluids, and quenches thirst.

[0065] Fresh Polygonatum: sweet in taste, neutral in nature. Enters the spleen, lung and kidney meridians. Replenishes qi and nourishes yin, strengthens the spleen and benefits the kidneys.

[0066] Fresh yam: sweet, neutral in nature. Enters the spleen, lung, and kidney meridians. Nourishes the spleen and stomach, promotes the production of body fluids and benefits the lungs, and nourishes the kidneys and astringes semen.

[0067] Fresh Radix Trichosanthis: sweet, slightly bitter, slightly cold in nature. Enters the lung and stomach meridians. Clears away heat and purges fire, promotes the production of body fluids and quenches thirst, and reduces swelling and discharges pus.

[0068] Fresh Ophiopogon japonicus: sweet, slightly bitter, slightly cold in nature. Enters the heart, lung, and stomach meridians. Nourishes yin, produces body fluid, moistens the lungs, and clears the heart.

[0069] Fresh ginseng: sweet, slightly bitter, slightly warm in nature. Enters the spleen, lung, heart, and kidney meridians. It greatly replenishes vital energy, restores pulse and strengthens the body, nourishes the spleen and lungs, promotes the production of body fluids and nourishes blood, and calms the mind and improves intelligence.

[0070] Fresh Astragalus: sweet in taste, slightly warm in nature. Enters the lung and spleen meridians. Replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes fluid production and nourishes blood, relieves stagnation and arthritis, removes toxins and discharges pus, and heals sores and promotes tissue regeneration.

[0071] Fresh ginger: spicy, slightly warm. Enters the lung, spleen, and stomach meridians. Relieves cold, warms the middle and stops vomiting, resolves phlegm and stops coughing.

[0072] Bombyx Batryticatus: salty and spicy in taste, neutral in nature. Enters the liver, lung and stomach meridians. Calms wind and stops spasms, dispels wind and stops pain, resolves phlegm and disperses stagnation.

[0073] The above components are scientifically and reasonably combined according to the principle of primary and secondary compatibility of TCM monarch, minister, assistant and envoy, and work together. Fresh ginseng greatly replenishes vital energy, nourishes the spleen and lungs, replenishes without being dry, promotes the healthy movement of the spleen and stomach and the filling of lung qi; fresh rehmannia nourishes yin and blood, clears heat and cools blood, can nourish yin fluid, moisturize dryness and quench thirst, and clear away deficiency heat. Fresh ginseng and fresh rehmannia are both monarch medicines, playing the dual role of replenishing qi and consolidating the body, nourishing yin and clearing heat; astragalus, as an important medicine for replenishing qi, can replenish qi and consolidate the exterior, detoxify and regenerate muscles. Fresh astragalus can raise yang and consolidate the exterior, stop sweating and regenerate muscles, while reducing the dryness of the dry product. When used together with ginseng, it can significantly enhance the effect of replenishing qi and make qi and blood abundant. Fresh Polygonatum can nourish Qi and Yin, strengthen the spleen and benefit the kidney, assist the nourishing Yin effect of fresh Rehmannia, and promote the transportation and transformation function of the spleen and stomach; fresh Astragalus and fresh Polygonatum are both ministerial drugs, which can assist in strengthening the effect of nourishing Qi and nourishing Yin; fresh Ophiopogon can nourish Yin and moisten the lungs, promote fluid and quench thirst, and can enhance the power of nourishing Yin; fresh Pueraria root has the effect of relieving muscle and fever, promoting fluid and quenching thirst, and the two can nourish Yin and promote fluid and quench thirst together; fresh Radix Trichosanthis can clear away heat and promote fluid, reduce swelling and discharge pus, and can be used with fresh Ophiopogon to enhance the effect of nourishing Yin and promoting fluid, effectively relieving the symptoms of thirst and dry throat; fresh Chinese yam can nourish the spleen and nourish the stomach; fresh ginger can warm the middle and strengthen the stomach; white silkworm can resolve phlegm and disperse knots, and treat symptoms caused by phlegm and heat, and meridian blockage. Fresh Ophiopogon japonicus, fresh Radix Trichosanthis, fresh Pueraria root, fresh Chinese yam, white silkworm and fresh ginger are used as adjuvant drugs. The combination of the medicines can replenish qi and strengthen the body, nourish yin and clear away heat, promote the production of body fluid and quench thirst, clear away heat and purge fire, and can be effectively used to treat type 2 diabetes with deficiency of both qi and yin, and has achieved very good beneficial technical effects. The relevant test data are as follows (taking Example 1 as an example):

[0074] 1. Case selection

[0075] Type 2 diabetes is also known as type 2 diabetes. Patients with high blood sugar will experience typical symptoms such as polydipsia, polyphagia, polyuria, weight loss, and physical fatigue. They may also be accompanied by symptoms such as dizziness, headache, itchy skin, numbness of the limbs, visual impairment, and ketoacidosis. Those with the above clinical symptoms can be used as sources of case selection.

[0076] 2. Diagnostic criteria

[0077] 2.1. Western medicine diagnosis refers to the "Guidelines for the Prevention and Treatment of Type 2 Diabetes in China (2020 Edition)" and the "Guidelines for the Diagnosis and Treatment of Type 2 Diabetes Disease and Syndrome" of the Diabetes Branch of the Chinese Medical Association. Patients have typical diabetes symptoms such as thirst, "three mores", weight loss, fasting blood sugar ≥ 7.0mmol / L and (or) 2h postprandial blood sugar ≥ 11.1mmol / L. Excluding type 1 diabetes, special types of diabetes, and gestational diabetes, they can be diagnosed with T2DM. TCM diagnosis refers to the "National Guidelines for the Prevention and Treatment of Diabetes in TCM at the Grassroots Level (2022)", which is mainly Qi and Yin deficiency syndrome. The patient's clinical manifestations are thirst, frequent drinking, fatigue, red tongue with little fluid, thin white or little fur, and a thin and rapid pulse. It can be diagnosed as hyperglycemia in type 2 diabetes with Qi and Yin deficiency syndrome.

[0078] 2.2 Inclusion criteria

[0079] ① Age between 18 and 70 years old; ② Meet the diagnostic criteria of Western medicine and traditional Chinese medicine; ③ Meet the requirements of medical ethics; ④ Patients give informed consent to participate in this study, can accept the treatment plan of this study, and sign the informed consent form to confirm.

[0080] 2.3 Exclusion criteria

[0081] ① Those aged <18 or >70 years old; ② Those who do not meet the diagnostic criteria and inclusion criteria; ③ Those who are unwilling to cooperate and suffer from serious diseases such as cardiovascular, liver, kidney or hematopoietic system or mental illness; ④ Those with serious complications such as diabetic nephropathy, diabetic retinopathy or type 1 diabetes; ⑤ Those who meet the inclusion criteria but have various complications, adverse reactions and other serious adverse events during the treatment and need to terminate this study or change the treatment plan.

[0082] 3. Treatment options

[0083] A total of 100 cases were observed, including 50 males and 50 females, with an average age of 46.5 years.

[0084] The confirmed patients were treated with the composition of the present invention, and a control group was set up during the treatment. 100 cases were randomly divided into 2 groups (experimental group and control group), with 50 people in each group.

[0085] Experimental group: Using the method of Example 1, weigh 30g of fresh Rehmannia root, 30g of fresh Pueraria root, 30g of fresh Polygonatum, 30g of fresh Chinese yam, 30g of fresh Radix Trichosanthis, 30g of fresh Radix Ophiopogonis, 30g of fresh Ginseng, 30g of fresh Astragalus, 2g of Bombyx Batryticatus and 4g of fresh ginger, prepare the Chinese medicine composition of the present invention, put them into capsules respectively, and prepare 18 capsules, each of which is about 0.5g. Take with warm water, three times a day, 6 capsules each time.

[0086] Control group: Metformin hydrochloride, oral, once a day, 2 tablets (0.5g) each time, taken during or after meals.

[0087] One course of treatment is 14 days, and the efficacy is observed after one course of treatment.

[0088] Measurement: The fasting blood glucose level and 2-hour postprandial blood glucose level of the patients were measured every Monday morning.

[0089] 4. Criteria for determining efficacy

[0090] The TCM symptom scores, fasting blood sugar, and 2-hour postprandial blood sugar of the two groups before and after treatment were observed and compared to evaluate the efficacy. Among them, the TCM syndrome score was scored using the diabetes syndrome grading scale. The main symptoms were dry mouth and throat and fatigue, each of which was scored 0 to 6 points according to the severity; the secondary symptoms were 5 items: eating more and getting hungry, thirsty and drinking, shortness of breath and lazy speech, five upset and hot, and palpitations and insomnia, which were scored 0 to 3 points according to the severity. The total score of the main and secondary symptoms was added to the TCM syndrome score, with a total score of 27 points. The higher the score, the more severe the patient's symptoms.

[0091] Markedly effective: Clinical symptoms and signs of TCM are significantly improved, with the syndrome score reduced by ≥70%; fasting blood sugar and 2-hour postprandial blood sugar drop to the normal range, or fasting blood sugar and 2-hour postprandial blood sugar drop by more than 40% of the pre-treatment level;

[0092] Effective: Clinical symptoms and signs of TCM are significantly improved, and the syndrome score is reduced by ≥30%; fasting blood sugar and 2-hour postprandial blood sugar drop to the normal range, or fasting blood sugar and 2-hour postprandial blood sugar drop by more than 20% before treatment, but it does not reach the marked effect standard;

[0093] Ineffective: fasting blood sugar and 2h postprandial blood sugar did not decrease or the decrease did not reach the effective standard.

[0094] 5. Statistical processing

[0095] According to the treatment scheme of the present invention, 100 outpatients diagnosed with the disease were randomly divided into an experimental group and a control group with 50 cases in a ratio of 1:1 using a random number table method: the experimental group had 34 females and 16 males, aged 28 to 69 years old, with a course of disease of 2 to 24 months; the control group had 35 females and 15 males, aged 29 to 70 years old, with a course of disease of 3 to 25 months. There was no significant difference in general data such as gender, age, and course of disease between the two groups of patients after statistical analysis (P>0.05), and they were comparable.

[0096] Statistical methods All data were processed by SPSS 22.0 software. t test was performed for count data, and chi-square test was performed for count data. 2 P<0.05 was considered statistically significant. The enumeration data were analyzed by statistic test, and the rank data were analyzed by Ridit test.

[0097] The blood sugar lowering effects are shown in Table 1.

[0098] Table 1 Changes in blood sugar levels and TCM symptom scores of the two groups of patients before and after treatment

[0099]

[0100] Table 2 Comparison of clinical efficacy between the two groups of patients [n (%)]

[0101]

[0102] The experimental results show that the total effective rate of reducing blood sugar in the experimental group (Chinese medicine composition of the present invention) is 90%, and the total effective rate of reducing blood sugar in the control group is 78% (see Table 2). At the same time, it can be seen from the experimental results in Table 1 that after taking the pharmaceutical composition of the present invention (experimental group), the average fasting blood sugar and average postprandial blood sugar of the patients are lower than those of the patients taking the control group, and are within the normal range. This shows that the pharmaceutical composition of the present invention has a good effect of reducing blood sugar, and the therapeutic effect is significantly better than metformin hydrochloride.

[0103] 6. Typical cases:

[0104] Case 1: Zhang, female, 49 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 7.2 mmol / l. After taking the present invention for one week, her blood sugar before meals was 5.0 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0105] Case 2: Wang, male, 63 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, his blood sugar before meals was 11.2 mmol / l. After taking the present invention for one week, his blood sugar before meals was 5.7 mmol / l. After continuous use, his blood sugar was maintained in the normal range.

[0106] Case 3: Li, male, 38 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, his blood sugar before meals was 9.5 mmol / l. After taking the present invention for one week, his blood sugar before meals was 5.2 mmol / l. After continuous use, his blood sugar was maintained in the normal range.

[0107] Case 4: Zhu, female, 47 years old, from XXX city, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 10.6 mmol / l. After taking the present invention for one week, her blood sugar before meals was 4.9 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0108] Case 5: Han, female, 35 years old, from XXX city, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 7.3 mmol / l. After taking the present invention for one week, her blood sugar before meals was 5.1 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0109] Case 6: Xu, male, 30 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, his blood sugar before meals was 11.4 mmol / l. After taking the present invention for one week, his blood sugar before meals was 4.7 mmol / l. After continuous use, his blood sugar was maintained in the normal range.

[0110] Case 7: Shang Moumou, female, 51 years old, from XXX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 9.8 mmol / l. After taking the present invention for one week, her blood sugar before meals was 5.0 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0111] Case 8: Ren, male, 38 years old, from XX city, Henan Province, suffered from type 2 diabetes. Before taking the present invention, his blood sugar before meals was 8.6 mmol / l. After taking the present invention for one week, his blood sugar before meals was 4.5 mmol / l. After continuous use, his blood sugar was maintained in the normal range.

[0112] Case 9: Sun, female, 60 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 11.3 mmol / l. After taking the present invention for one week, her blood sugar before meals was 4.9 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0113] Case 10: Zhao Moumou, female, 42 years old, from XX City, Henan Province, suffered from type 2 diabetes. Before taking the present invention, her blood sugar before meals was 12.1 mmol / l. After taking the present invention for one week, her blood sugar before meals was 5.1 mmol / l. After continuous use, her blood sugar was maintained in the normal range.

[0114] While conducting experiments on Example 1, the same experiments were also conducted on other examples, and the same or similar results were obtained, which will not be listed here one by one.

[0115] 7. Conclusion

[0116] In summary, the pharmaceutical composition of the present invention is compatible according to the primary and secondary relationship of monarch, minister, assistant and envoy, and the prescription is scientific and reasonable. Fresh ginseng and fresh rehmannia are used to replenish qi and strengthen the foundation, nourish yin and clear away heat; fresh astragalus has less dryness than dry products, and is used together with fresh polygonatum to enhance the effect of replenishing qi and nourishing yin; fresh ophiopogon and fresh kudzu root are used together to nourish yin, promote fluid production and quench thirst, and are compatible with fresh trichosanthes to enhance the effect of nourishing yin and promoting fluid production, effectively relieving thirst and dry throat; fresh yam, white silkworm and fresh ginger are then used as adjuvants, and all the medicines are used together to replenish qi and strengthen the foundation, nourish yin and clear heat, promote fluid production and quench thirst, clear heat and purge fire, and can be effectively used to treat patients with qi and yin deficiency type T2DM, correct blood sugar, reduce complications, and improve quality of life, with an effective rate of up to 90%. At the same time, the Chinese medicine composition of the present invention forms a stable fresh medicine preparation process. After wall breaking and pulverization, the effective ingredients of each raw material medicine can be fully released, making full use of them, saving medicinal material resources, having the effect of lowering blood sugar, significantly improving the therapeutic effect, having no toxic side effects, and being able to keep blood sugar within a normal range. Moreover, the Chinese medicine composition of the present invention can be prepared into pharmaceutically permitted dosage forms such as granules, tablets and capsules according to actual needs, having strong practical application value, being conducive to industrial production and development, promotion and application, and having huge economic and social benefits.

Claims

1. A Chinese medicine composition for lowering blood sugar, which is prepared from the following raw materials by weight: 10-60 parts of fresh Rehmannia root, 10-60 parts of fresh Pueraria root, 10-60 parts of fresh Polygonatum, 10-60 parts of fresh Chinese yam, 10-60 parts of fresh Radix Trichosanthis, 10-60 parts of fresh Radix Ophiopogonis, 10-60 parts of fresh Ginseng, 10-60 parts of fresh Astragalus, 10-100 parts of Bombyx Batryticatus, 1-5 parts and 2-6 parts of fresh ginger, and the preparation method is: (1) After selecting the fresh Rehmannia root, wash it 1 to 3 times, cut it into pieces or slices while it is fresh, add water 0.5 to 4 times the weight volume of the original drug, the weight volume refers to the solid in g, and the liquid in mL, break the wall for 1 to 10 minutes, filter, and centrifuge the filtrate at 2000 to 8000 r / min for 5 to 30 minutes to obtain the first supernatant; then add water and repeat the above method of breaking the wall and centrifuging 1 to 4 times to obtain the second to fifth supernatants, and combine the supernatants of each time; After selecting, wash the fresh kudzu root 1-3 times, cut into sections or slices while fresh, add water 1-5 times the weight and volume of the original drug, break the wall for 1-20 minutes, filter, and centrifuge the filtrate at 2000-8000 r / min for 5-30 minutes to obtain the first supernatant; then add water and repeat the above method of breaking the wall and centrifuging 1-4 times to obtain the second to fifth supernatants, and combine the supernatants of each time; Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx batryticatus medicinal materials, freeze-dry them, grind them into coarse powder, pass them through an 80-100 mesh sieve, and then ultrafine grind them and pass them through a 150-300 mesh sieve to obtain ultrafine Bombyx batryticatus powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a finished product of a Chinese medicine composition for lowering blood sugar.

2. The method for preparing the hypoglycemic Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 30 parts of fresh rehmannia root, 30 parts of fresh kudzu root, 30 parts of fresh polygonatum, 30 parts of fresh yam, 30 parts of fresh trichosanthes root, 30 parts of fresh ophiopogon, 30 parts of fresh ginseng, 30 parts of fresh astragalus, 2 parts of white silkworm and 4 parts of fresh ginger. The preparation method is: (1) The fresh Rehmannia root is selected and washed twice, and cut into 2-4 mm thick slices while fresh, and water with a volume of 1 times the weight of the original drug is added, the cell wall is broken for 2.5 minutes, filtered, and the filtrate is centrifuged at 4000 r / min for 20 minutes to obtain the first supernatant; then 3 times the amount of water is added and the cell wall is broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time are combined; Fresh kudzu root was selected and washed twice, and cut into 2-4 mm thick slices while fresh, and water twice the weight volume of the original drug was added to break the wall for 5 minutes, filtered, and the filtrate was centrifuged at 4000 r / min for 20 minutes to obtain the first supernatant; then 3 times the amount of water was added and the wall was broken and centrifuged twice according to the above method to obtain the second and third supernatants, and the supernatants of each time were combined; Fresh kudzu root, fresh polygonatum, fresh yam, fresh trichosanthes root, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger are prepared into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and fresh ginseng is prepared into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx Batryticatus medicinal material into sections, freeze-dry, first grind into coarse powder, pass through a 100-mesh sieve, then ultrafine grind, pass through a 300-mesh sieve, and obtain ultrafine Bombyx Batryticatus powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

3. The method for preparing the hypoglycemic Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh rehmannia root, 20 parts of fresh kudzu root, 20 parts of fresh polygonatum, 20 parts of fresh yam, 20 parts of fresh trichosanthes root, 20 parts of fresh ophiopogon, 10 parts of fresh ginseng, 10 parts of fresh astragalus, 1 part of white silkworm and 2 parts of fresh ginger. The preparation method is: (1) The fresh Rehmannia root is selected and washed once, and cut into 2-4 mm thick slices while fresh, and water 3 times the weight volume of the original drug is added to break the cell wall for 5 minutes, filtered, and the filtrate is centrifuged at 2000 r / min for 30 minutes to obtain the first supernatant; then water is added and the cell wall is broken and centrifuged again according to the above method to obtain the second supernatant, and the supernatants of each time are combined; Fresh kudzu root was selected and washed once, and cut into 2-4 mm thick slices while fresh, and water 4 times the weight and volume of the original drug was added, and the cell wall was broken for 10 minutes, filtered, and the filtrate was centrifuged at 2000 r / min for 30 minutes to obtain the first supernatant; then water was added and the cell wall was broken and centrifuged again according to the above method to obtain the second supernatant, and the supernatants of each time were combined; Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx batryticatus medicinal material into sections, freeze-dry, first grind into coarse powder, pass through a 100-mesh sieve, and then ultrafine grind and pass through a 220-mesh sieve to obtain Bombyx batryticatus ultrafine powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

4. The method for preparing the hypoglycemic Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh rehmannia root, 25 parts of fresh kudzu root, 25 parts of fresh polygonatum, 25 parts of fresh yam, 25 parts of fresh trichosanthes root, 25 parts of fresh ophiopogon, 15 parts of fresh ginseng, 15 parts of fresh astragalus, 2 parts of white silkworm and 3 parts of fresh ginger. The preparation method is: (1) The fresh Rehmannia root is selected and washed once, cut into 2-4 mm thick slices while fresh, and added with water 4 times the weight volume of the original drug, the cell wall is broken for 10 min, filtered, and the filtrate is centrifuged at 8000 r / min for 20 min to obtain a supernatant; Fresh kudzu root was selected and washed once, cut into 2-4 mm thick slices while fresh, added with water 5 times the weight volume of the original drug, broken for 20 minutes, filtered, and the filtrate was centrifuged at 8000 r / min for 20 minutes to obtain the first supernatant; Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx batryticatus medicinal material into sections, freeze-dry it, first grind it into coarse powder, pass it through an 80-mesh sieve, then grind it into ultrafine powder, pass it through a 150-mesh sieve, and obtain ultrafine Bombyx batryticatus powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

5. The method for preparing the hypoglycemic Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 60 parts of fresh rehmannia root, 60 parts of fresh kudzu root, 60 parts of fresh polygonatum, 60 parts of fresh yam, 30 parts of fresh trichosanthes root, 30 parts of fresh ophiopogon, 60 parts of fresh ginseng, 60 parts of fresh astragalus, 5 parts of white silkworm and 6 parts of fresh ginger. The preparation method is: (1) The fresh Rehmannia root is selected and washed twice, cut into pieces while fresh, and added with water of 1 times the weight volume of the original drug, broken for 2 minutes, filtered, and the filtrate is centrifuged at 6000 r / min for 15 minutes to obtain the first supernatant; then 2 times the amount of water is added and the cell wall is broken and centrifuged three times according to the above method to obtain the second to fourth supernatants, and the supernatants of each time are combined; Fresh kudzu root was selected and washed twice, cut into sections while fresh, and water twice the weight of the original drug was added to break the wall for 3 minutes, filtered, and the filtrate was centrifuged at 6000 r / min for 15 minutes to obtain the first supernatant; then 2 times the amount of water was added and the wall was broken and centrifuged three times according to the above method to obtain the second to fourth supernatants, and the supernatants of each time were combined; Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx batryticatus medicinal material into sections, freeze-dry it, first grind it into coarse powder, pass it through an 80-mesh sieve, then grind it into ultrafine powder, pass it through a 150-mesh sieve, and obtain ultrafine Bombyx batryticatus powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

6. The method for preparing the hypoglycemic Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 40 parts of fresh rehmannia root, 40 parts of fresh kudzu root, 40 parts of fresh polygonatum, 40 parts of fresh yam, 10 parts of fresh trichosanthes root, 10 parts of fresh ophiopogon japonicus, 20 parts of fresh ginseng, 20 parts of fresh astragalus, 3 parts of white silkworm and 5 parts of fresh ginger. The preparation method is: (1) The fresh Rehmannia root is selected and washed once, cut into pieces while fresh, and 0.5 times the weight volume of water is added to break the wall for 1 minute, filtered, and the filtrate is centrifuged at 8000 r / min for 5 minutes to obtain the first supernatant; then 2 times the amount of water is added and the wall is broken and centrifuged again according to the above method for 4 times to obtain the second to fifth supernatants, and the supernatants of each time are combined; Fresh kudzu root was selected and washed once, cut into segments while fresh, and water with a volume of 1 times the weight of the original drug was added, the cell wall was broken for 1 min, filtered, and the filtrate was centrifuged at 8000 r / min for 5 min to obtain the first supernatant; then 2 times the amount of water was added and the cell wall was broken and centrifuged again according to the above method for 4 times to obtain the second to fifth supernatants, and the supernatants of each time were combined; Prepare fresh polygonatum, fresh yam, fresh radix trichosanthis, fresh ophiopogon japonicus, fresh ginseng, fresh astragalus and fresh ginger into corresponding supernatants according to the preparation method of fresh rehmannia root supernatant, and prepare fresh ginseng into corresponding supernatants according to the preparation method of fresh kudzu root supernatant; (2) ultrafiltration of each supernatant obtained in step (1) using a 10000D ultrafiltration membrane to remove macromolecular substances, collecting each ultrafiltration permeate, and then concentrating using a 200D nanofiltration membrane to obtain each nanofiltration concentrate; then freeze-drying each nanofiltration concentrate, collecting each freeze-dried product, and pulverizing to obtain each freeze-dried powder; (3) Wash and cut the Bombyx batryticatus medicinal material into sections, freeze-dry, first grind into coarse powder, pass through an 80-mesh sieve, then ultrafine grind, pass through a 180-mesh sieve, and obtain Bombyx batryticatus ultrafine powder; (4) The freeze-dried powders obtained in step (2) and the Bombyx batryticatus superfine powder obtained in step (3) are uniformly mixed, vacuum-packed, and sterilized to obtain a Chinese medicine composition for lowering blood sugar.

7. Use of the hypoglycemic Chinese medicinal composition prepared by the method according to any one of claims 1 to 6 in the preparation of hypoglycemic drugs.

8. Use of the hypoglycemic Chinese medicine composition prepared by the method according to claim 7 in the preparation of hypoglycemic drugs, characterized in that: The medicine is a granule, tablet or capsule prepared by adding a pharmaceutically acceptable auxiliary material to a traditional Chinese medicine composition for reducing blood sugar.

9. Use of the blood sugar lowering Chinese medicinal composition prepared by the method according to any one of claims 1 to 6 in preparing drugs for preventing, alleviating and treating abnormal blood sugar.

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