Process for producing indoleacetic acid organic fertilizer by utilizing microalgae
Through the microalgae process, pig manure, Chlorella nucleus liquor and black soldier fly larvae are mixed and treated. The black soldier fly larvae produces indole acetic acid and supplements potassium elements are supplemented, which solves the problems of complex existing processes and environmental pollution, and achieves the realization of the problem of indole acetic acid. Preparation of efficient organic fertilizer.
Patent Information
- Application Number
- CN202411606672.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-01-07
AI Technical Summary
The existing indole acetic acid synthesis process is complex and harmful to the environment. There is less potassium in pig manure, and there is a problem of ineffective loss of nitrogen during insect treatment.
Using microalgae technology, pig manure, Chlorella nucleus liquor and black soldier fly larvae are mixed and treated. The black soldier fly larvae produces indole acetic acid during protein catalysis and metabolism, and Chlorella nucleus supplements potassium elements to achieve Preparation of organic fertilizer.
It realizes efficient production of indole acetic acid, reduces the volatile loss of nitrogen, supplements potassium, and prepares organic fertilizers rich in nitrogen, phosphorus and potassium, simplifies the process flow and reduces environmental pollution.
Smart Images
Figure CN119263896A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of organic fertilizer production, and more specifically to a process for producing indoleacetic acid organic fertilizer by utilizing microalgae. Background Art
[0002] Indole-3-acetic acid is the main active ingredient of natural plant auxin and an organic compound naturally produced in plants. It mainly regulates the plant's phototropism, geotropism, apical dominance, fruit development and other physiological processes by promoting cell elongation, division and differentiation. Indole-3-acetic acid plays an irreplaceable role in key links such as seed germination, stem elongation, and root geotropism. By regulating the distribution and transportation of nutrients in plants, indole-3-acetic acid ensures that plants can efficiently use resources and achieve optimal growth.
[0003] At present, indoleacetic acid is mainly synthesized by the following method: indole, formaldehyde and potassium cyanide react at a high temperature of 150°C and a high pressure of 0.9-1 MPa to generate 3-indoleacetonitrile, which is then hydrolyzed under the action of potassium hydroxide, or indole reacts with glycolic acid to obtain it. However, the above method of obtaining indoleacetic acid is complicated and easily causes adverse effects on the environment.
[0004] In addition, plant fertilizers need to be rich in nitrogen, phosphorus and potassium, while pig manure contains more nitrogen and phosphorus and less potassium, and there is a problem of ineffective loss of nitrogen during the insect treatment process. Summary of the invention
[0005] The technical problem to be solved by the present invention is to provide a process for producing indoleacetic acid organic fertilizer by using microalgae in view of the above problems that the process for preparing indoleacetic acid is complicated and affects the environment.
[0006] The technical solution of the present invention to solve the above technical problems is to provide a process for producing indoleacetic acid organic fertilizer using microalgae, the process comprising the following steps:
[0007] A: Put pig manure, pyrenoid chlorella algae liquid and black soldier fly larvae into the treatment pool and stir them evenly. The concentration of the pyrenoid chlorella algae liquid is not less than 1×10 8 The dosage of the Chlorella algae solution is 10-30 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 600,000-1.2 million larvae per ton of pig manure;
[0008] B: adding pig manure and Chlorella pyrenoidosa liquid to the treatment pool at preset times;
[0009] C: At a preset time after the last addition of pig manure and Chlorella vulgaris liquid, black soldier fly larvae and insect sand are separated, and organic fertilizer containing indoleacetic acid is obtained by processing the insect sand.
[0010] As a further improvement of the present invention, in step B, the amount of pig manure and Chlorella vulgaris liquid added to the treatment pool each time is the same as the amount added in step A.
[0011] As a further improvement of the present invention, the organic fertilizer containing indoleacetic acid is obtained by processing insect sand, which includes: drying the separated insect sand in a shaded and ventilated place for 2-4 days, and recovering the insect sand when the moisture content drops to 20-40%, to obtain the organic fertilizer containing indoleacetic acid.
[0012] As a further improvement of the present invention, step B comprises: adding pig manure and Chlorella vulgaris liquid to the treatment pool on the 3rd, 6th and 9th day after executing step A, respectively; the preset time in step C is the 11th to 21st day.
[0013] As a further improvement of the present invention, the step B comprises: maintaining the ambient temperature of the treatment pool at 20-35° C. and stirring the treatment pool once a day.
[0014] As a further improvement of the present invention, the pig manure added in step A and step B is whole pig manure with a moisture content of 80-90%, and the depth of the pig manure in step A is 1-5 cm.
[0015] As a further improvement of the present invention, the individual size of the black soldier fly larvae put into the treatment pool in step A is 0.02-0.06g.
[0016] As a further improvement of the present invention, the step C further comprises: freezing or drying the separated black soldier fly larvae to obtain animal feed.
[0017] As a further improvement of the present invention, step A comprises: adjusting the depth of pig manure to 1-3 cm, adding 15-25 liters of the protein core Chlorella algae solution per ton of pig manure, adding 800,000-1,000,000 black soldier fly larvae per ton of pig manure, and the individual size of the black soldier fly larvae is 0.03-0.05 g.
[0018] As a further improvement of the present invention, the preset time in step C is the 16th-31st day, and the organic fertilizer containing indoleacetic acid is obtained by processing insect sand, including: drying the separated insect sand in a shaded and ventilated place for 3-4 days, and recovering the insect sand when the moisture content of the insect sand drops to 25-35%, so as to obtain the organic fertilizer containing indoleacetic acid.
[0019] The invention has the following beneficial effects: by adding pyrenoid chlorella to pig manure treated with black soldier fly larvae, the pyrenoid chlorella can provide protein nutrition for the black soldier fly larvae while absorbing ammonia nitrogen and phosphate in the pig manure, and the black soldier fly larvae produce indoleacetic acid in the process of decomposing tryptophan in protein, and the potassium element in the chlorella can make up for the problem of insufficient potassium element in the pig manure sand, and finally, the preparation of organic fertilizer containing indoleacetic acid and nitrogen, phosphorus and potassium elements is completed while the pig manure is treated. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 The present invention provides a schematic flow chart of a process for producing indoleacetic acid organic fertilizer using microalgae according to an embodiment of the present invention. DETAILED DESCRIPTION
[0021] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0022] Chlorella pyrenoidosa has the ability of photosynthetic autotrophy and chemoheterotrophic reproduction, and can use pollutants such as ammonia nitrogen to synthesize protein in the body, reaching 50-65% of dry weight. The present invention utilizes the photosynthetic and pollution control capabilities of Chlorella pyrenoidosa to assist in the degradation of pig manure while providing protein nutrition for black soldier fly larvae, improving the survival rate and weight gain rate of black soldier fly larvae, and promoting the production of indoleacetic acid.
[0023] like Figure 1 , is a flow chart of a process for producing indoleacetic acid organic fertilizer using microalgae provided by an embodiment of the present invention. The process of this embodiment includes the following steps:
[0024] Step S1: Put pig manure, pyrenoid chlorella algae liquid and black soldier fly larvae into the treatment pool and stir them evenly. The concentration of the pyrenoid chlorella algae liquid is not less than 1×10 8 The dosage of the Chlorella algae liquid is 10-30 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 600,000-1.2 million larvae per ton of pig manure.
[0025] To facilitate temperature control, the above-mentioned treatment pool can be an indoor (or greenhouse) treatment pool with natural light or artificial light. To improve the preparation efficiency, the pig manure added in this step is whole pig manure with a water content of 80-90%, and to improve the photosynthesis efficiency of Chlorella pyrenoidosa, the depth of the pig manure in the treatment pool is 1-5 cm. Preferably, the depth of the pig manure in the treatment pool is 1-3 cm.
[0026] In order to improve the survival rate of black soldier fly larvae and the decomposition efficiency of tryptophan in protein, the individual size of the black soldier fly larvae put into the treatment pool in this step is 0.02-0.06g.
[0027] Step S2: adding pig manure and pyrenoid chlorella algae solution to the treatment pool at preset times. Specifically, in this step, the amount of pig manure and pyrenoid chlorella solution added to the treatment pool each time is the same as the amount added in step S1. Of course, in actual applications, the amount of pig manure and pyrenoid chlorella solution added each time can also be adjusted according to the on-site conditions (such as light, temperature, etc.).
[0028] In one embodiment of the present invention, pig manure and Chlorella pyrenoidosa liquid can be added to the treatment in multiple times, for example, pig manure and Chlorella pyrenoidosa liquid can be added to the treatment pool on the 3rd, 6th and 9th day after step S1 is performed. The pig manure added in this step is also whole pig manure with a water content of 80-90%, and the thickness of the added pig manure is ensured to be uniform as much as possible.
[0029] Furthermore, in order to improve the efficiency of generating indoleacetic acid, the ambient temperature of the treatment pool needs to be maintained at 20-35°C, and the treatment pool needs to be stirred once a day. Preferably, the ambient temperature of the treatment pool is maintained at 25-32°C.
[0030] Step S3: at a preset time after the last addition of pig manure and Chlorella vulgaris liquid, the black soldier fly larvae and insect sand are separated, and the organic fertilizer containing indoleacetic acid is obtained by processing the insect sand.
[0031] Since the black soldier fly larvae not only generate indoleacetic acid during the catabolism of tryptophan in protein, but also consume indoleacetic acid, the preset time in this step is the 11th to 21st day. For example, when pig manure and protein-core Chlorella algae liquid are added to the treatment pool on the 3rd, 6th and 9th days in step S2, the entire preparation cycle is 20-30 days, so that the indoleacetic acid content in the separated insect sand reaches a higher level.
[0032] In this step, the separated insect sand can be air-dried in a sunshade and ventilated place for 2-4 days, and recovered when the moisture content of the insect sand drops to 20-40%, to obtain an organic fertilizer containing indoleacetic acid. The organic fertilizer containing indoleacetic acid can be directly used as a plant fertilizer and is easy to store.
[0033] In addition, the black soldier fly larvae separated and obtained in this step can be frozen or dried for preservation and used as animal feed.
[0034] The above-mentioned process of using microalgae to produce indoleacetic acid organic fertilizer is to add pig manure and protein nucleus chlorella algae liquid to the black soldier fly treatment pool in the process of pig manure treatment according to the sequencing batch method, so that the protein nucleus chlorella provides protein nutrition for the black soldier fly larvae while absorbing ammonia nitrogen and phosphate in the pig manure, and the black soldier fly larvae produce indoleacetic acid in the process of decomposition and metabolism of tryptophan in protein, and the preparation of organic fertilizer containing indoleacetic acid is completed while the pig manure treatment is realized. The use of protein nucleus chlorella can not only improve the survival rate and yield of black soldier fly larvae, but also promote the production of indoleacetic acid in insect sand, thus becoming an effective method for strengthening the resource treatment efficiency of black soldier flies. In addition, in the above-mentioned process, by introducing protein nucleus chlorella, the ability of protein nucleus chlorella to assimilate nitrogen elements can be used to reduce the volatilization loss of nitrogen elements, and at the same time, with the help of potassium elements in chlorella, the problem of insufficient potassium elements in pig manure insect sand is supplemented, thereby laying the foundation for the production of insect sand organic fertilizer rich in nitrogen, phosphorus and potassium elements.
[0035] In one embodiment of the present invention, the above step S1 includes: adjusting the depth of pig manure to 1-3 cm, and making the dosage of pyrenoid chlorella algae liquid to be 15-25 liters per ton of pig manure, and the dosage of black soldier fly larvae to be 800,000-1 million per ton of pig manure, and the individual size of the black soldier fly larvae is 0.03-0.05g. In this way, the content of indoleacetic acid in organic fertilizer can be further increased.
[0036] In one embodiment of the present invention, the preset time in the above step S3 is the 16th to 31st day, and the organic fertilizer containing indoleacetic acid is obtained by processing the insect sand, including: drying the separated insect sand in a shaded and ventilated place for 3-4 days, and recovering the insect sand when the moisture content drops to 25-35%, to obtain the organic fertilizer containing indoleacetic acid. In this way, the content of indoleacetic acid in the organic fertilizer can be further increased.
[0037] The following are several embodiments of the process for producing indoleacetic acid organic fertilizer using microalgae.
[0038] Example 1
[0039] Step S11: Use whole pig manure with a moisture content of 80% and put it into the treatment pool. The depth of the pig manure reaches 5 cm. Add 30 liters of water with a concentration of not less than 1×10 8 Mix the Chlorella algae liquid with 10000 liters of protein core and stir evenly, add black soldier fly larvae at a ratio of 1.2 million larvae per ton of pig manure, and the individual size of the larvae reaches 0.02-0.04g.
[0040] Step S12: On the 3rd, 6th and 9th days of treatment, add pig manure and Chlorella vulgaris liquid in the same amount as in step S11 to the treatment pool, maintain the ambient temperature at 20-30°C, and stir the treatment pool once a day to allow the black soldier fly larvae to continue feeding on pig manure and grow.
[0041] Step S13: On the 25th day of treatment (i.e., the 16th day after the last addition of pig manure and Chlorella vulgaris liquid), the black soldier fly larvae and insect sand are separated manually or mechanically, the obtained larvae are frozen or dried for storage and used as animal feed, and the insect sand is spread out and air-dried for 2 days, and recovered after the moisture content drops to 30%, to obtain insect sand organic fertilizer containing indoleacetic acid.
[0042] The results showed that the wet weight yield of black soldier fly larvae was 36.7 kg / ton of pig manure, the worm sand yield was 305.8 kg / ton of pig manure, and the indoleacetic acid content in the worm sand reached 77.3 mg / kg (dry basis), the nitrogen content was 39.7 mg / kg (dry basis), the phosphorus content was 35.3 mg / kg (dry basis), and the potassium content was 40.4 mg / kg (dry basis).
[0043] Example 2
[0044] Step S21: Use whole pig manure with a moisture content of 85% and put it into the treatment pool. The depth of the pig manure reaches 3 cm. Add 20 liters of water with a concentration of not less than 1×10 8 Mix 100000 larvae of Chlorella vulgaris liquid per liter, stir evenly, add black soldier fly larvae at a ratio of 900,000 larvae per ton of pig manure, and the individual size of the larvae reaches 0.02-0.05g.
[0045] Step S22: On the 3rd, 6th and 9th days of treatment, add pig manure and Chlorella vulgaris liquid in the same amount as in step S21 to the treatment pool, maintain the ambient temperature at 25-35°C, and stir the treatment pool once a day to allow the black soldier fly larvae to continue feeding on pig manure and grow.
[0046] Step S23: On the 20th day of the treatment (i.e., the 11th day after the last addition of pig manure and Chlorella vulgaris liquid), the black soldier fly larvae and insect sand are separated manually or mechanically, the obtained larvae are frozen or dried for storage and used as animal feed, and the insect sand is spread out and air-dried for 3 days, and recovered after the moisture content drops to 35%, to obtain insect sand organic fertilizer containing indoleacetic acid.
[0047] The results showed that the wet weight yield of black soldier fly larvae was 31.3 kg / ton of pig manure, the worm sand yield was 259.5 kg / ton of pig manure, and the indoleacetic acid content in the worm sand reached 56.1 mg / kg (dry basis), the nitrogen content was 36.2 mg / kg (dry basis), the phosphorus content was 34.1 mg / kg (dry basis), and the potassium content was 35.3 mg / kg (dry basis).
[0048] Example 3
[0049] Step 31: Use whole pig manure with a moisture content of 90% and put it into the treatment pool. The depth of the pig manure reaches 1 cm. Add 10 liters of water with a concentration of not less than 1×10 8 Mix 100000 larvae / L of Chlorella algae liquid, stir evenly, add black soldier fly larvae at a ratio of 600,000 larvae per ton of pig manure, and the individual size of the larvae reaches 0.03-0.06g.
[0050] Step S32: On the 3rd, 6th and 9th days of treatment, add pig manure and Chlorella vulgaris liquid in the same amount as in step S31 to the treatment pool, maintain the ambient temperature at 27-35°C, and stir the treatment pool once a day to allow the black soldier fly larvae to continue feeding on pig manure and grow.
[0051] Step S33: On the 30th day of the treatment (i.e., the 21st day after the last addition of pig manure and Chlorella vulgaris liquid), the black soldier fly larvae and insect sand are separated manually or mechanically, the obtained larvae are frozen or dried for storage and used as animal feed, and the insect sand is spread out and air-dried for 4 days, and recovered after the moisture content drops to 40%, to obtain insect sand organic fertilizer containing indoleacetic acid.
[0052] As a result, the wet weight yield of black soldier fly larvae was 22.8kg / ton of pig manure, the worm sand yield was 220.8kg / ton of pig manure, the indoleacetic acid content in the worm sand reached 23.7mg / kg (dry basis), the nitrogen content was 32.7mg / kg (dry basis), the phosphorus content was 33.2mg / kg (dry basis), and the potassium content was 28.2mg / kg (dry basis).
[0053] Comparative Example 1
[0054] Step S41: Use whole pig manure with a moisture content of 85% and put it into the treatment pool. The depth of the pig manure reaches 1-5 cm, and black soldier fly larvae are added at a ratio of 800,000 larvae per ton of pig manure, and the individual size of the larvae reaches the requirement of 0.04g.
[0055] Step S42: On the 3rd, 6th and 9th days of treatment, add an amount of pig manure equal to that in step S41 to the treatment pool, maintain the ambient temperature at 25-32°C, and stir the treatment pool once a day to allow the black soldier fly larvae to continue feeding on the pig manure and grow.
[0056] Step S43: On the 27th day of treatment (i.e., the 18th day after the last addition of pig manure and Chlorella vulgaris liquid), the black soldier fly larvae and insect sand are mechanically separated, the obtained larvae are frozen or dried for storage and used as animal feed, and the insect sand is spread out and air-dried for 4 days, and then recovered after the moisture content drops to 40%, to obtain insect sand organic fertilizer.
[0057] The results showed that the wet weight yield of black soldier fly larvae was 12.3 kg / ton of pig manure, the worm sand yield was 201 kg / ton of pig manure, no indoleacetic acid was detected in the worm sand, the nitrogen content was 28.1 mg / kg (dry basis), the phosphorus content was 36.4 mg / kg (dry basis), and the potassium content was 17.5 mg / kg (dry basis).
[0058] It can be seen that by adding Chlorella vulgaris liquid when treating pig manure with black soldier flies, the production of indoleacetic acid can be effectively promoted, and by controlling the process time, organic fertilizer containing indoleacetic acid and nitrogen, phosphorus and potassium elements can be obtained.
[0059] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.
Claims
1. A process for producing indoleacetic acid organic fertilizer using microalgae, characterized in that: The process comprises the following steps: A: Put pig manure, pyrenoid chlorella algae liquid and black soldier fly larvae into the treatment pool and stir them evenly. The concentration of the pyrenoid chlorella algae liquid is not less than 1×10 8 The dosage of the Chlorella algae solution is 10-30 liters per ton of pig manure, and the dosage of the black soldier fly larvae is 600,000-1.2 million larvae per ton of pig manure; B: adding pig manure and Chlorella pyrenoidosa liquid to the treatment pool at preset times; C: At a preset time after the last addition of pig manure and Chlorella vulgaris liquid, black soldier fly larvae and insect sand are separated, and organic fertilizer containing indoleacetic acid is obtained by processing the insect sand.
2. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: In the step B, the amount of pig manure and Chlorella vulgaris liquid added to the treatment pool each time is the same as the amount added in the step A.
3. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The method of obtaining the organic fertilizer containing indoleacetic acid by treating the insect sand comprises: drying the separated insect sand in a shaded and ventilated place for 2-4 days, and recovering the insect sand when the moisture content is reduced to 20-40%, so as to obtain the organic fertilizer containing indoleacetic acid.
4. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The step B comprises: adding pig manure and Chlorella vulgaris liquid to the treatment pool on the 3rd, 6th and 9th day after the step A is performed, respectively; the preset time in the step C is the 11th to 21st day.
5. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The step B comprises: maintaining the ambient temperature of the treatment pool at 20-35° C. and stirring the treatment pool once a day.
6. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The pig manure added in step A and step B is whole pig manure with a moisture content of 80-90%, and the depth of the pig manure in step A is 1-5 cm.
7. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The individual size of the black soldier fly larvae put into the treatment pool in step A is 0.02-0.06g.
8. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The step C also includes: freezing or drying the separated black soldier fly larvae to obtain animal feed.
9. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The step A comprises: adjusting the depth of pig manure to 1-3 cm, adding 15-25 liters of the protein core Chlorella algae solution per ton of pig manure, adding 800,000-1,000,000 black soldier fly larvae per ton of pig manure, and the individual size of the black soldier fly larvae is 0.03-0.05 g.
10. The process for producing indoleacetic acid organic fertilizer using microalgae according to claim 1, characterized in that: The preset time in step C is the 16th to 31st day, and the organic fertilizer containing indoleacetic acid is obtained by processing the insect sand, including: drying the separated insect sand in a shaded and ventilated place for 3-4 days, and recovering the insect sand when the moisture content drops to 25-35%, to obtain the organic fertilizer containing indoleacetic acid.