Automatic sampling pipeline system and control method

Abstract

The invention discloses an automatic sampling pipeline system and a control method. Full automation of automatic sampling, automatic vibration dyeing, automatic sample introduction and automatic cleaning is completed through the cleaning pump, the sample pump, the feeding pump, the dye pump and the second waste liquid pump, and a guarantee is provided for subsequent fluorescence analysis treatment. The system provided by the invention has an automatic uniform mixing function, manual auxiliary uniform mixing is not needed, liquid can be conveyed in two directions, and cleaning is sufficient. The automation degree is high, the failure rate is low, and the requirement of continuous sample injection can be met.

Description

Technical Field

[0001] The invention relates to an automatic sampling system, and in particular to an automatic sampling pipeline system and a control method matched with an online milk somatic cell fluorescence analysis system. Background Art

[0002] Fluorescence analysis is a technique for qualitative and quantitative analysis based on the phenomenon of molecular fluorescence. The principle is to use fluorescent dyes to mark specific components in cells. After absorbing light of a specific wavelength (called excitation light), these fluorescent molecules will transition to a high-energy state. Subsequently, they quickly return to the ground state and emit light with a longer wavelength (called fluorescence). This emitted light is processed by filters and optical systems, amplified and recorded, and ultimately forms a fluorescent image of the sample. By observing these images using a fluorescence microscope, the distribution of fluorescent markers in the sample and their dynamic changes can be analyzed. Fluorescence analysis is widely used in various cell analyses. Taking somatic cell detection as an example, when using an instrument to analyze and detect samples, the conventional method is to manually use a syringe or pipette to inject a small amount of sample into a specific detection pool or detection chip, and then use the instrument for automatic analysis.

[0003] In the field of fluorescence analysis, compared with the manual injection method in traditional laboratories, automatic injection can ensure the accuracy and consistency of each injection volume through mechanized and standardized operations, greatly reduce human errors, and improve the accuracy and repeatability of the results. It can automatically and continuously inject multiple samples without manual intervention, significantly improving the sample processing speed and experimental efficiency, and is particularly outstanding in high-throughput analysis. At the same time, since the sample is processed in a closed system, the chance of the sample being exposed to the external environment is reduced, the risk of sample contamination is reduced, and direct contact with personnel is avoided when handling harmful, toxic or corrosive samples, thereby improving operational safety. It is particularly suitable for high-sensitivity analysis and trace sample processing. It is worth mentioning that many automatic injection devices can be pre-programmed to adapt to complex experimental processes and diverse sample processing requirements. They are highly flexible and can adapt to different experimental needs. Therefore, the automatic injection system has become an indispensable and important tool for modern fluorescence analysis.

[0004] The rapid development of automatic sampling systems is due to the progress of precision machining technology, micro motors and transmission devices, the application of various high-precision miniaturized intelligent sensors, modern control theory and automation technology, as well as microfluidics technology and new materials. The comprehensive application and continuous progress of these technologies have promoted the development of automatic sampling devices, making them play an increasingly important role in various types of analysis and testing and laboratory applications.

[0005] In the field of fluorescence analysis, specifically in the field of somatic cell detection and counting, although the technology is developing rapidly, the mainstream method of sampling is still manual sampling, especially some instruments used for teaching and scientific research, which rarely have the functions of automatic sampling and online detection. The automation level of fluorescence analysis instruments on the market is low. During the analysis and testing process, multiple sampling and injection are usually required. If manual injection is used, there will be problems such as low accuracy, poor repeatability and stability, and low efficiency. When responding to rapid detection events, most of the processes are performed manually, which will cause greater safety hazards when using some toxic reagents.

[0006] In contrast, a more ideal injection method is automatic injection. Currently, there are various automatic injection systems on the market, such as automatic pipetting injection system, pipeline transfer injection system, and flow cytometer automatic injection system. Among these injection systems, there is a technology that uses a more traditional six-way valve combined with a water pump. This technology has the problems of easy aging of ordinary water pumps, lack of automatic shaking steps, manual assistance is required to shake the samples, samples are prone to cross-contamination, and the single-direction delivery of liquids leads to insufficient cleaning. There is also a more precise injection method, which is the automatic pipetting injection technology using an injection plate, a combined robotic arm and an injection needle. However, this technology is difficult to integrate, complex to control, has a high system failure rate, and occupies too much space. The design of this injection turntable structure is widely used in automatic injection devices, but often lacks a process for real-time automatic cleaning of the supporting pipelines and liquid needles.

[0007] Manual injection is easily affected by the operator's technical level, hand shaking and environmental changes, resulting in inaccurate and inconsistent injection volume, which affects the accuracy and repeatability of experimental results. The manual injection process is cumbersome, especially when analyzing a large number of samples, it is time-consuming and inefficient. In addition, samples are susceptible to contamination, especially when dealing with trace or high-sensitivity samples. Manual injection also increases the operator's exposure risk when dealing with toxic, harmful or corrosive samples. At present, fields such as fluorescence analysis are still dominated by manual injection, and there is a lack of an automatic injection technology with simple control, high accuracy and perfect process.

[0008] Therefore, it is a technical problem to be solved urgently to propose an automatic sampling system with wide applicability, high stability, perfect process, easy integration and high degree of automation. Summary of the invention

[0009] In order to solve the problems and needs existing in the background technology, the present invention proposes an automatic sampling pipeline system and a control method.

[0010] The technical solution adopted by the present invention is:

[0011] 1. An automatic sampling pipeline system

[0012] The automatic sampling pipeline system includes a second cleaning pump, a sample pump, a feeding pump, a dye pump, a second waste liquid pump, a clean water bottle, a sample bottle, a dye bottle, a waste liquid bottle and a mixing pool; the input port of the second cleaning pump is connected to the clean water bottle, and the output port of the second cleaning pump is connected to the inlet of the mixing pool; the input port of the sample pump is connected to the sample bottle, and the output port of the sample pump is connected to the inlet of the mixing pool; the outlet of the mixing pool is connected to the switch valve through a check valve, the input port of the feeding pump is connected to the outlet of the mixing pool through the switch valve, and the output port of the feeding pump is connected to the detection area; the input port of the dye pump is connected to the dye bottle, and the output port of the dye pump is connected to the inlet of the mixing pool; the input port of the second waste liquid pump is connected to the outlet of the mixing pool through the switch valve, and the output port of the second waste liquid pump is connected to the waste liquid bottle.

[0013] It also includes a first cleaning pump and a first waste liquid pump. The input port of the first cleaning pump is connected to the clean water bottle, and the output port of the first cleaning pump is connected to the sample bottle; the input port of the first waste liquid pump is connected to the sample bottle, and the output port of the first waste liquid pump is connected to the waste liquid bottle.

[0014] The switch valve comprises a two-position three-way valve.

[0015] The detection area is an observation chamber in a microfluidic chip, and a sample is placed in the microfluidic chip.

[0016] The first cleaning pump, the second cleaning pump, the sample pump, the loading pump, the dye pump, the first waste liquid pump and the second waste liquid pump include one of a six-roller peristaltic pump head and a four-roller peristaltic pump head.

[0017] A vibration motor is also arranged near the pipeline of the mixing pool, which is used to shake the liquid at the input pipeline of the mixing pool into the mixing pool and automatically vibrate the mixing pool body.

[0018] The sample bottle includes a milk reservoir.

[0019] The first cleaning pump, the second cleaning pump, the sample pump, the feeding pump, the dye pump, the first waste liquid pump and the second waste liquid pump are all connected to the host computer through a microcontroller.

[0020] There are multiple sample bottles, and the multiple sample bottles are connected to the input port of the sample pump through a multi-way pipeline.

[0021] 2. A control method for an automatic sampling pipeline system

[0022] Control the automatic sampling pipeline system to mix and deliver samples until the sample delivery in the detection area is completed;

[0023] Control the automatic sampling pipeline system to perform pipeline self-cleaning.

[0024] The control of the automatic sampling pipeline system to perform mixed sample delivery until the sample delivery to the detection area is completed includes the following steps:

[0025] Step 1: Start the sample pump and dye pump to pump the sample and dye respectively until the sample and dye fill the corresponding pipes respectively, then vibrate the pipe at the entrance of the mixing pool to shake the liquid at the pipe mouth into the mixing pool, then start the second cleaning pump to inject clean water into the mixing pool and clean the residual liquid, and then start the second waste liquid pump to pump away the waste liquid after cleaning;

[0026] Step 2: Start the sample pump and dye pump, and pump the sample and dye to the mixing pool through the pipeline at the same time according to the preset ratio. After the fluid is stable, the system in the mixing pool is automatically vibrated and mixed for dyeing;

[0027] Step 3: Switch the state of the check valve, drive the loading pump to pump the mixed liquid to the detection area at the set rate; drive the sample pump and the dye pump to withdraw the remaining sample and dye in the pipeline;

[0028] Step 4: Switch the state of the check valve so that the mixing pool is connected to the second waste liquid pump, and start the second waste liquid pump to pump out the excess waste liquid in the mixing pool to the waste liquid bottle;

[0029] Step 5: Repeat steps 1 to 4 until the sample delivery to the inspection area is completed.

[0030] The control of the automatic sampling pipeline system to perform pipeline self-cleaning includes:

[0031] Step 1: Start the second cleaning pump to inject clean water into the mixing pool, vibrate the mixing pool, and then start the second waste liquid pump to extract the waste liquid from the mixing pool;

[0032] Step 2: Start the second cleaning pump to inject clean water into the mixed sample pool again, switch the state of the check valve, start the feeding pump to pump the clean water to the detection area, then reset the check valve, and start the second waste liquid pump to pump excess waste liquid into the waste liquid bottle;

[0033] Step 3: Drive the sample pump, dye pump and second cleaning pump to withdraw the remaining liquid in the pipeline.

[0034] The controlling the automatic sampling pipeline system to perform pipeline self-cleaning also includes:

[0035] Start the first cleaning pump to inject clean water into the sample bottle. After completion, start the first waste liquid pump to pump the waste liquid from the sample bottle into the waste liquid bottle.

[0036] The beneficial effects of the present invention are:

[0037] The present invention proposes an automatic sampling pipeline system, which overcomes the defects of manual sampling and the low degree of automation and insufficient stability and accuracy of the prior art, has high precision and repeatability, multi-module integration, and significantly improves the processing speed and analysis efficiency.

[0038] The present invention adopts a fully automatic process of automatic sampling, automatic staining, automatic injection and automatic cleaning, and the cleaning provides a guarantee for subsequent fluorescence analysis processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic diagram of the structure of the automatic sampling pipeline system proposed by the present invention.

[0040] Figure 2 Schematic diagram of the piping for injection operation.

[0041] Figure 3 Schematic diagram of the pipeline for pumping away waste liquid.

[0042] Figure 4 Schematic diagram of the piping for system cleaning operations.

[0043] Figure 5 This is the control framework diagram of the automatic sampling pipeline system.

[0044] In the figure: a first cleaning pump 1, a second cleaning pump 2, a sample pump 3, a feeding pump 4, a dye pump 5, a first waste liquid pump 6, a second waste liquid pump 7, a clean water bottle 8, a sample bottle 9, a dye bottle 10, a waste liquid bottle 11, a mixing pool 12, and a check valve 15. DETAILED DESCRIPTION

[0045] The present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0046] The present invention proposes an automatic sampling pipeline system with high accuracy and repeatability. It uses a microcontroller combined with a host computer for automatic control, which significantly improves the processing speed and analysis efficiency. Through the fully automatic process of automatic sampling, automatic vibration staining, automatic sampling, and automatic cleaning, it provides guarantee for subsequent fluorescence analysis and processing. Compared with the existing technology, it has an automatic mixing function, does not require manual auxiliary mixing, liquid can be transmitted in both directions, and is fully cleaned. It has a high degree of automation and a low failure rate, and can meet the requirements of continuous sampling.

[0047] like Figure 1As shown, an automatic sampling pipeline system proposed in the present invention includes a first cleaning pump 1, a first waste liquid pump 6, a second cleaning pump 2, a sample pump 3, a feeding pump 4, a dye pump 5, a second waste liquid pump 7, a clean water bottle 8, a sample bottle 9, a dye bottle 10, a waste liquid bottle 11 and a mixing pool 12; the input port of the first cleaning pump 1 is connected to the clean water bottle 8, and the output port of the first cleaning pump 1 is connected to the sample bottle 9; the input port of the first waste liquid pump 6 is connected to the sample bottle 9, and the output port of the first waste liquid pump 6 is connected to the waste liquid bottle 11. The input port of the second cleaning pump 2 is connected to the clean water bottle 8, and the output port of the second cleaning pump 2 is connected to the inlet of the mixing pool 12; the input port of the sample pump 3 is connected to the sample bottle 9, and the output port of the sample pump 3 is connected to the inlet of the mixing pool 12; the outlet of the mixing pool 12 is connected to the switch valve through the check valve 15, the input port of the loading pump 4 is connected to the outlet of the mixing pool 12 through the switch valve, and the output port of the loading pump 4 is connected to the detection area; the input port of the dye pump 5 is connected to the dye bottle 10, and the output port of the dye pump 5 is connected to the inlet of the mixing pool 12; the input port of the second waste liquid pump 7 is connected to the outlet of the mixing pool 12 through the switch valve, and the output port of the second waste liquid pump 7 is connected to the waste liquid bottle 11.

[0048] Among them, one end of the first cleaning pump 1 and the second cleaning pump 2 is connected to the clean water bottle 8 for pumping clean water. The pump is relatively large, so the accuracy is lower than that of the sample pump 3, the feeding pump 4, and the dye pump 5. The size of the inner tube of the pump is selected to be 3×5mm inner diameter × outer diameter. The sample pump 3, the feeding pump 4, and the dye pump 5 are connected to the mixing tank 12, which has certain requirements for accuracy. The nozzle is equipped with a check valve. The silicone tube in the pump is preferably 0.4×3mm inner diameter × outer diameter, and the flow rate range is 0.0002-3ml / min. The peristaltic pump is driven by a stepper motor. The stepper motor can provide consistent performance for the system, ensure that the operation results are the same each time, and accurately control the position by adjusting the input pulse signal to achieve adjustable precise metering and quantitative delivery. One end of the pipeline of the first waste liquid pump 6 and the second waste liquid pump 7 is connected to the waste liquid bottle 11, which is mainly used to pump the waste liquid after cleaning. It does not need to accurately control the flow rate, so it is driven by a DC motor.

[0049] The water bottle 8, sample bottle 9, dye bottle 10 and waste liquid bottle 11 are designed with bottle caps of different sizes of silicone hoses in actual implementation, which are strictly fixed to the hoses when the pumps are working, and are easy to use. The pipelines of the sample pump 3 and the dye pump 5 are connected to the sample bottle 9 and the dye bottle 10 at one end respectively, and the peristaltic pump is driven by a microcontroller to generate a specific pulse signal to extract the liquid in the bottle to the mixing pool 12. It should be noted that vibration motors are provided on both sides of the mixing pool 12, and the internal rotor speed of the motor can reach 22,000 rpm. Due to the presence of an eccentric block on the rotor, the centrifugal force generated during high-speed rotation causes the entire motor to vibrate, which can play a good mixing role on the quantitative sample and dye system in the mixing pool to ensure the dyeing effect.

[0050] The switch valve is a two-position three-way valve. The mixing pool 12 is connected to the common end of a two-position three-way solenoid valve 18 through a check valve 15. The check valve is used to prevent liquid backflow, is small in size, and is easy to disassemble. One end of the vibration motor is connected to the electromagnetic relay module 21, and the microcontroller sends high and low level signals to the control port to realize the circuit on-off control. The two-position three-way solenoid valve 18 has three ports, namely the common end, the normally open end and the normally closed end. Here, the normally closed end is connected to the second waste liquid pump 7 through a pipeline for subsequent extraction of waste liquid, and the normally open end is connected to the peristaltic feeding pump 4 for pumping the dyed sample. The IO port of the microcontroller is connected to the electromagnetic three-way valve control foot, and the on-off power of the electromagnetic coil in the electromagnetic valve is controlled by outputting high and low levels, thereby controlling the action of the valve core to realize the on-off and regulation of the fluid. The on-off of the port is adjusted in time at different stages of the injection process, and different pumps are driven accordingly to extract the liquid in the mixing pool. The use of an electromagnetic three-way valve can well seal and isolate the fluid from the actuator, and realize accurate and reliable fluid transmission.

[0051] The sample bottle 9 includes a milk pool. The detection area includes an observation chamber in the microfluidic chip, and a sample is placed in the flow channel of the microfluidic chip.

[0052] The first cleaning pump 1, the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5, the first waste liquid pump 6 and the second waste liquid pump 7 are all connected to the host computer through a microcontroller.

[0053] The first cleaning pump 1, the second cleaning pump 2, the sample pump 3, the feeding pump 4, the dye pump 5, the first waste liquid pump 6 and the second waste liquid pump 7 include one of a six-roller peristaltic pump head and a four-roller peristaltic pump head. The peristaltic pump effectively avoids cross contamination by pumping liquid so that the liquid only contacts the pump tube and does not contact other parts of the pump. The pump tube is easy to clean and replace, maintenance is simple, and bidirectional pumping can be performed. The peristaltic pump has a small shear force on the liquid, which is suitable for transporting biological samples and cell suspensions, etc., without destroying its structure. Compared with the three-roller pump head, the six-roller pump head increases the number of rollers, which can reduce the pulsation and fluctuation of the fluid, and is very suitable for applications that require a stable flow rate. More rollers can also reduce the gap between the rollers, thereby reducing liquid reflux and recoil, improving pumping efficiency, and also means a reduction in pipeline pressure, which can reduce pipeline wear and fatigue.

[0054] There are multiple sample bottles 9 , and the multiple sample bottles 9 are connected to the input port of the sample pump 3 through a multi-way pipeline.

[0055] A vibration motor is also provided near the pipeline of the mixing pool 12, which is used to shake the liquid at the input pipeline of the mixing pool 12 into the mixing pool 12 and automatically vibrate the body of the mixing pool 12, so as to complete the mixing and dyeing.

[0056] The system uses a switching power supply with a rated power of 200W and an adjustable output of 12V / 24V. It has overload, overvoltage and short-circuit protection functions, which are sufficient to drive five stepper motors to work normally. In addition, the system components also include terminal blocks for wiring, 12V to 5V step-down modules for power supply, eight-way relay modules for controlling the on and off of the circuit, and act as an electrical switch. The left plate of the frame and the bottom plate play a fixing role.

[0057] Since the pipeline needs to be filled with liquid before sampling, during routine testing, the pipeline can be simply cleaned while the new sample is filling the pipeline. The pipeline can also be inserted into a clean water bottle to draw it back, or the peristaltic pump can be used to remove the pipeline for deep cleaning.

[0058] The microcontroller uses the STM32F103ZET6 chip, which integrates 512KB of Flash memory and 64KB of SRAM, with an operating frequency of up to 72MHz and a rich peripheral interface, including up to 112 GPIO pins, 12-bit ADC, DAC, I 2 C, SPI, USART and CAN bus interfaces. In addition, it also supports USB 2.0 full-speed interface and multiple timers, suitable for complex real-time control and communication tasks. The speed of the stepper motor is planned by software to achieve closed-loop drive and further improve the stability of the motor. Appropriate software delay in the middle of the step ensures the full progress of the action process.

[0059] The system is connected to the host computer through the serial port and receives signals from the host computer. During the implementation process, when mixed sample delivery is required, the host computer sends BCJ1\r\n to indicate the start, and the lower computer sends BCJ2\r\n to indicate the completion of sample delivery. In single sample delivery, the host computer sends BCJ3\r\n to indicate the start, and the lower computer sends BCJ4\r\n to indicate the completion of single sample delivery. In the cleaning step, the host computer sends BCJ5\r\n to indicate the start of the cleaning step, and the lower computer sends BCJ6\r\n to indicate the completion of the cleaning process. If a process is not cleaned sufficiently, the host computer can send a control signal to control a certain process to continue separately to ensure the completion of the process. The system is equipped with a travel electromagnet, which acts on the waste liquid pipeline, with a stroke of 10mm and a push-pull force of up to 65N. The host computer controls the microcontroller to send a switch signal to the relay control coil to turn on and off the power, so that the push rod moves to press the pipe connected to the outlet of the observation room to ensure that the liquid does not flow back through the pipe. The silicone pipe is not easy to fatigue or break, can adapt to the force of repeated compression and loosening, and the travel electromagnet bracket is designed separately to cooperate with the pipeline.

[0060] like Figure 5 As shown, a control method of an automatic sampling pipeline system proposed by the present invention includes:

[0061] Control the automatic sampling pipeline system to mix and deliver samples until the sample delivery in the detection area is completed;

[0062] Control the automatic sampling pipeline system to perform pipeline self-cleaning.

[0063] Controlling the automatic sampling pipeline system to mix and deliver samples until the delivery of samples to the detection area is completed includes the following steps:

[0064] Step 1: The check valve 15 is in the OFF state. The host computer sends a start signal, first starting the sample pump 3 and the dye pump 5 to pump the sample and dye respectively for a period of time, until the sample and dye fill the corresponding pipelines respectively, so as to facilitate the subsequent synchronous adjustable volume injection. Then, the pipeline at the entrance of the mixing pool 12 is vibrated by a vibration motor for 0.5s to shake the liquid at the pipe mouth into the mixing pool 12, and then the second cleaning pump 2 is started to inject clean water into the mixing pool 12 and clean the residual liquid, and then the second waste liquid pump 7 is started to extract the waste liquid after cleaning;

[0065] Step 2: If Figure 2 As shown, the sample pump 3 and the dye pump 5 are started, and the sample and the dye are simultaneously pumped to the mixing pool 12 through the pipeline according to a preset ratio (such as 4:1), and the microcontroller generates a switch signal after a delay of 5 seconds to wait for the fluid to stabilize, so that the vibration motor rigidly connected to the mixing pool is energized for 5 seconds, and then the system in the mixing pool 12 is automatically vibrated, mixed and dyed;

[0066] Step 3: The loading pump 4 is connected to the mixing pool 12 and the detection area through a pipeline. After the mixing is completed, the state of the check valve 15 is switched, specifically, the check valve 15 is switched to the ON state, and the microcontroller sends a pulse signal to drive the loading pump 4 to pump the mixed liquid to the detection area at the set rate; the microcontroller flips the control level of the driver direction control port of the sample pump 3 and the dye pump 5, and drives the sample pump 3 and the dye pump 5 to withdraw the remaining small amount of sample and dye in the pipeline, in order to shorten the waiting time for the fluid flow rate to slow down, so as to facilitate photo counting. Then the lower computer sends a sample delivery completion signal to the upper computer, and the upper computer can automatically take photos and count;

[0067] Step 4: Switch the state of the check valve 15, that is, switch the check valve 15 to the OFF state, so that the mixing pool 12 is connected to the second waste liquid pump 7, the stroke electromagnet is turned on, and the observation chamber outlet pipeline is clamped to prevent the waste liquid from flowing back. Figure 3 As shown, the second waste liquid pump 7 is started to pump the excess waste liquid in the mixing pool 12 to the waste liquid bottle 11;

[0068] Step 5: Repeat steps 1 to 4 until the sample delivery in the detection area is completed. The upper computer sends a single sample delivery signal, the stroke electromagnet is disconnected and reset, the feeding pump 4 is started to deliver the sample once, and then the stroke electromagnet is turned on, and the lower computer sends a single sample delivery completion signal to the upper computer.

[0069] Control the automatic sampling pipeline system to perform pipeline self-cleaning, including:

[0070] Step 1: The host computer sends a cleaning signal, starts the second cleaning pump 2 to inject clean water into the mixed sample pool 12, and after the vibration motor is powered on and vibrates for 2.5 seconds to vibrate the mixed sample pool 12, the second waste liquid pump 7 is started to extract the waste liquid from the mixed sample pool 12;

[0071] Step 2: If Figure 4 As shown, the second cleaning pump 2 is started to inject clean water into the mixed sample pool 12 again, the state of the check valve 15 is switched, specifically, the check valve 15 is switched to the ON state, the feeding pump 4 is started to pump clean water to the detection area for cleaning the pipeline and the observation room, and then the check valve 15 is reset, and the second waste liquid pump 7 is started to pump excess waste liquid into the waste liquid bottle 11;

[0072] Step 3: Start the first cleaning pump 1 to quickly inject clean water into the sample bottle 9 , and after completion, start the first waste liquid pump 6 to pump the waste liquid from the sample bottle 9 into the waste liquid bottle 11 .

[0073] Step 4: The microcontroller flips the control level of the driver direction control port of the sample pump 3, the dye pump 5, and the second cleaning pump 2, and drives the first cleaning pump 1, the sample pump 3, the dye pump 5, and the second cleaning pump 2 to pump back the small amount of liquid remaining in the pipeline. Then the lower computer sends a cleaning completion signal.

[0074] The above steps ensure the efficiency, accuracy and reliability of the system's automatic sampling, mixing, staining, detection and cleaning processes. The main idea of ​​the system is that the microcontroller communicates with the host computer, and through the host computer control signal, controls the solenoid valves, motors, vibration motors and other components in the system, thereby realizing the functions of automatic sampling, automatic staining vibration, automatic sampling and automatic cleaning.

[0075] Finally, it should be noted that the above embodiments and explanations are only used to illustrate the technical solution of the present invention rather than to limit it. Those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the spirit and scope disclosed in the technical solution of the present invention, which should be included in the scope of protection of the claims of the present invention.

Claims

1. An automatic sampling pipeline system, characterized in that: The invention comprises a second cleaning pump (2), a sample pump (3), a feeding pump (4), a dye pump (5), a second waste liquid pump (7), a clean water bottle (8), a sample bottle (9), a dye bottle (10), a waste liquid bottle (11) and a sample mixing pool (12); the input port of the second cleaning pump (2) is connected to the clean water bottle (8), the output port of the second cleaning pump (2) is connected to the inlet of the sample mixing pool (12); the input port of the sample pump (3) is connected to the sample bottle (9), and the output port of the sample pump (3) is connected to the inlet of the sample mixing pool (12); The outlet of the sample mixing pool (12) is connected to the switch valve via a check valve (15); the input port of the feeding pump (4) is connected to the outlet of the sample mixing pool (12) via the switch valve; the output port of the feeding pump (4) is connected to the detection area; the input port of the dye pump (5) is connected to the dye bottle (10); the output port of the dye pump (5) is connected to the inlet of the sample mixing pool (12); the input port of the second waste liquid pump (7) is connected to the outlet of the sample mixing pool (12) via the switch valve; the output port of the second waste liquid pump (7) is connected to the waste liquid bottle (11).

2. The automatic sampling pipeline system according to claim 1, characterized in that: It also comprises a first cleaning pump (1) and a first waste liquid pump (6), wherein the input port of the first cleaning pump (1) is connected to a clean water bottle (8), and the output port of the first cleaning pump (1) is connected to a sample bottle (9); the input port of the first waste liquid pump (6) is connected to the sample bottle (9), and the output port of the first waste liquid pump (6) is connected to a waste liquid bottle (11).

3. The automatic sampling pipeline system according to claim 1, characterized in that: The switch valve comprises a two-position three-way valve.

4. The automatic sampling pipeline system according to claim 1, characterized in that: The detection area is an observation chamber in the microfluidic chip.

5. The automatic sampling pipeline system according to claim 1, characterized in that: The first cleaning pump (1), the second cleaning pump (2), the sample pump (3), the loading pump (4), the dye pump (5), the first waste liquid pump (6) and the second waste liquid pump (7) include one of a six-roller peristaltic pump head and a four-roller peristaltic pump head.

6. The automatic sampling pipeline system according to claim 1, characterized in that: A vibration motor is also arranged near the pipeline at the mixing pool (12) for shaking the liquid at the input pipeline of the mixing pool (12) into the mixing pool (12) and for automatically vibrating the body of the mixing pool (12).

7. The control method of an automatic sampling pipeline system according to claim 2, characterized in that: The control method comprises: Control the automatic sampling pipeline system to mix and deliver samples until the sample delivery in the detection area is completed; Control the automatic sampling pipeline system to perform pipeline self-cleaning.

8. The control method of an automatic sampling pipeline system according to claim 7, characterized in that: The control of the automatic sampling pipeline system to perform mixed sample delivery until the sample delivery to the detection area is completed includes the following steps: Step 1: Start the sample pump (3) and the dye pump (5) to pump the sample and the dye respectively until the sample and the dye fill the corresponding pipes respectively, then vibrate the pipe at the entrance of the mixing pool (12) to shake the liquid at the pipe mouth into the mixing pool (12), then start the second cleaning pump (2) to inject clean water into the mixing pool (12) and clean the residual liquid, and then start the second waste liquid pump (7) to pump away the waste liquid after cleaning; Step 2: Start the sample pump (3) and the dye pump (5), and pump the sample and the dye to the mixing tank (12) through the pipeline at the same time according to the preset ratio. After the fluid is stable, the system in the mixing tank (12) is automatically vibrated and mixed for dyeing; Step 3: Switch the state of the check valve (15), drive the loading pump (4) to pump the mixed liquid to the detection area at a set rate; drive the sample pump (3) and the dye pump (5) to withdraw the remaining sample and dye in the pipeline; Step 4: Switch the state of the check valve (15) so that the sample mixing pool (12) is connected to the second waste liquid pump (7), and start the second waste liquid pump (7) to pump the excess waste liquid in the sample mixing pool (12) into the waste liquid bottle (11); Step 5: Repeat steps 1 to 4 until the sample delivery to the inspection area is completed.

9. The control method of an automatic sampling pipeline system according to claim 7, characterized in that: The control of the automatic sampling pipeline system to perform pipeline self-cleaning includes: Step 1: Start the second cleaning pump (2) to inject clean water into the sample mixing pool (12), vibrate the sample mixing pool (12), and then start the second waste liquid pump (7) to extract the waste liquid from the sample mixing pool (12); Step 2: Start the second cleaning pump (2) to inject clean water into the mixing pool (12) again, switch the state of the check valve (15), start the feeding pump (4) to pump the clean water to the detection area, then reset the check valve (15), and start the second waste liquid pump (7) to pump excess waste liquid into the waste liquid bottle (11); Step 3: Drive the sample pump (3), the dye pump (5) and the second cleaning pump (2) to draw back the remaining liquid in the pipeline.

10. The control method of the automatic sampling pipeline system according to claim 9, characterized in that: The controlling the automatic sampling pipeline system to perform pipeline self-cleaning also includes: The first cleaning pump (1) is started to inject clean water into the sample bottle (9), and after completion, the first waste liquid pump (6) is started to pump the waste liquid from the sample bottle (9) into the waste liquid bottle (11).

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