Diaphorina citri drug resistance monitoring device and drug resistance determination method

Abstract

The invention relates to the field of plant protection, and discloses a diaphorina citri drug resistance monitoring device and a drug resistance determination method.The diaphorina citri drug resistance monitoring device comprises a fixing frame, the top end of the fixing frame is fixedly connected with a camera obscura, one end of the camera obscura fixedly communicates with a transparent connecting pipe, one end of the transparent connecting pipe fixedly communicates with a pesticide spraying box, and one end of the camera obscura is provided with a moving groove; a shading plate is slidably connected into the moving groove, a first connecting rope is fixedly connected to the top end of the shading plate, a reel is wound around the end, penetrating into the camera obscura, of the first connecting rope, a first motor is fixedly connected to one end of the reel, the first motor is fixedly connected with the camera obscura, and an infrared counter is fixedly connected to one end of the camera obscura. Compared with the prior art, the workload of an operator is reduced, the working efficiency is improved, pesticide liquid is sprayed to diaphorina citri through the pesticide spraying assembly arranged in the pesticide spraying box, pesticide liquid volatilization during manual pesticide liquid spraying is avoided, and the operation safety is improved.

Description

Technical Field

[0001] The invention relates to the field of plant protection, and in particular to a citrus psyllid pesticide resistance monitoring device and a pesticide resistance determination method. Background Art

[0002] Citrus psyllid is an insect of the family Psyllidae in the order Hemiptera. The adult citrus psyllid is small, about 3 mm long. It feeds and lives on the veins and tender shoots on the back of leaves. It is phototactic and has strong cold resistance. It can safely overwinter when the monthly average temperature is above 6.2℃. It remains motionless when the temperature is below 8℃, and can fly and jump at 14℃. Citrus psyllid mainly harms Rutaceae plants, with Citrus being the worst affected.

[0003] In the prior art, a Chinese patent document with publication number CN114794030B proposes a citrus psyllid resistance monitoring device and a method for determining resistance, which solves the problem that the leaf dipping method is used to determine the resistance of citrus psyllid, the work is cumbersome, the number of psyllids treated in each tube is too small, the workload is large when batch experiments are required, and the efficiency is low. However, in actual application, the leaf dipping method can only detect two types of drugs, systemic and stomach poison. In order to improve the use effect of the drug, there is also a type of drug that takes into account contact killing, systemic and stomach poison. When this type of drug is tested, the use of the leaf dipping method will affect the results and affect the accuracy of the results. The existing pesticide resistance detection also adopts the spray method. When in use, the pesticide will escape into the air. The adult citrus psyllid is small in size. In order to observe whether the adult body is sprayed with the pesticide, the operator needs to observe them one by one at close range. In this process, the pesticide escaped in the air may be inhaled by the operator, which is not conducive to the safety of the operator. In addition, there are multiple adults in many groups of experiments. After spraying the pesticide solution, the adults need to be transferred to the culture bottle in turn, which is too inefficient and time-consuming. Therefore, we disclose a citrus psyllid pesticide resistance monitoring device and a pesticide resistance determination method to meet the needs of improving the use safety and determination efficiency of the spray method. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the shortcomings of the prior art, the present invention provides a citrus psyllid resistance monitoring device and a method for determining resistance, which have the advantages of improving the safety of use and determination efficiency of the spray method, and solve a series of problems such as poor safety and low efficiency in detecting citrus psyllid adults using the spray method.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for monitoring citrus psyllid resistance to pesticides, comprising a fixed frame, a dark box is fixedly connected to the top of the fixed frame, a transparent connecting tube is fixedly connected to one end of the dark box, a spray box is fixedly connected to one end of the transparent connecting tube, a movable groove is opened at one end of the dark box, a shading plate is slidably connected in the movable groove, a first connecting rope is fixedly connected to the top of the shading plate, the first connecting rope passes through one end of the dark box and is wound with a reel, one end of the reel is fixedly connected to a first motor, the first motor is fixedly connected to the dark box, one end of the dark box is fixedly connected to an infrared counter, the infrared counter is located at the upper end of the transparent connecting tube, a spray assembly is arranged in the spray box, the spray assembly is used to spray liquid medicine on citrus psyllid to avoid volatilization of liquid medicine during manual spraying, and the spray assembly is connected to the spray box.

[0008] Preferably, the spray assembly includes a conveyor belt group rotatably connected to the spray box, a plurality of spray pipes and cold air pipes are fixedly connected to the top of the spray box, the conveyor belt group is located at the lower ends of the spray pipes and the cold air pipes, one end of the conveyor belt group is located below one end of the transparent connecting pipe, a conveyor plate is placed below the other end of the conveyor belt group, one end of the conveyor plate is rotatably connected to the spray box, a third motor is fixedly connected to one end of the spray box, and the output shaft of the third motor passes through the spray box and is fixedly connected to the conveyor belt group.

[0009] Preferably, the other end of the conveying plate is fixedly connected to a second connecting rope, the top end of the second connecting rope passes through the outside of the spray box and is fixedly connected to a moving frame, the top end of the spray box is fixedly connected to a moving track, the moving frame is slidably connected to the moving track, the middle part of the moving frame is rotatably connected to a first connecting rod, one end of the first connecting rod is rotatably connected to a first turntable, the middle part of the first turntable is fixedly connected to a first belt group, and one end of the first belt group is fixedly sleeved with a second motor.

[0010] Preferably, one end of the first belt group is rotatably connected to the second belt group, the lower end of the second belt group is fixedly connected to the second turntable, one end of the second turntable is rotatably connected to the second connecting rod, one end of the second connecting rod is rotatably connected to the connecting frame, the middle part of the connecting frame is fixedly connected to a connecting block, the lower end of the spray box is fixedly connected to a limiting frame, the connecting block is slidably connected in the limiting frame, the bottom end of the connecting frame is fixedly connected to a sealing plug, and the sealing plug fits with the bottom end of the spray box.

[0011] Preferably, the side surface of the movable track is T-shaped, the first turntable is rotatably connected to the spray box, and the second motor is fixedly connected to the spray box.

[0012] Preferably, the connecting block is a T-block, the sealing plug is a hemisphere, the spray box includes a square box at the upper end and a tubular box at the lower end, the other end of the conveying plate is located at the upper end of the tubular box of the spray box, the transparent connecting pipe is inclined, and the temperature of the gas sprayed from the spray box is below 8°C.

[0013] Preferably, the lower end of the fixed frame is rotatably connected to the third belt group, the lower end of the fixed frame is fixedly connected to the fourth motor, the output shaft of the fourth motor passes through the fixed frame and is fixedly sleeved with the third belt group, a culture bottle is placed on the upper end of the third belt group, the upper end of the culture bottle is threadedly connected to a cap, both ends of the cap are rotatably connected to a mesh plate, the lower end of the mesh plate is fixedly connected to a limit spring, the lower end of the limit spring is fixedly connected to the cap, and the two mesh plates are both semicircular.

[0014] Preferably, the two mesh plates can form a full circle.

[0015] Preferably, a plurality of groups of limit push blocks are fixedly connected to the outer side of the third belt group, and the limit push blocks are adapted to the culture bottles. The two ends of the fixed frame are respectively fixedly connected to the first placement box and the second placement box, and the bottom surfaces of the first placement box and the second placement box are at a certain angle to the horizontal plane. The holes opened in the middle of the fixed frame are adapted to the culture bottles. The first placement box and the second placement box are fixedly connected to a guide plate on one side close to the fixed frame, and the second placement box is fixedly connected to a buffer frame on one side, and the buffer frame includes a baffle and a spring telescopic rod.

[0016] A method for determining the insecticide resistance of citrus psyllid, which is specifically applied to the above-mentioned citrus psyllid insecticide resistance monitoring device, comprises the following steps:

[0017] S1, placing the adult insects in the dark box, starting the first motor to drive the shading plate to move upward through the reel and the first connecting rope, so that the adult insects are attracted by the light transmitted through the transparent connecting tube and crawl into the transparent connecting tube, and the infrared counter counts at the same time. When the set number is reached, the first motor is driven to reverse, so that the shading plate falls down under its own gravity, closing the passage between the dark box and the transparent connecting tube;

[0018] S2, at this time, the cold air pipe starts to spray in the spray box, so that the adult insects stop moving and slide down the transparent connecting pipe to the upper end of the conveyor belt group, and then the conveyor belt group drives the adult insects to move to the bottom of the spray pipe and start spraying according to the set spray;

[0019] S3. After spraying, the conveyor belt group continues to rotate and drives the adult insects to the upper end of the conveyor plate. Then the second motor drives the first belt group and the second belt group to rotate, so that the second connecting rope drives the conveyor plate to rotate, so that the adult insects on the upper end of the conveyor plate fall along the conveyor plate. At this time, the culture bottle is located at the lower end of the spray box, and the mesh plate is opened under the push of the sealing plug, so that the falling adult insects fall into the culture bottle. Then the mesh plate is closed, and the temperature in the culture bottle is room temperature. The adult insects will resume activity. Then the culture bottle is placed in an artificial climate box and re-fed for a certain number of days to check the survival rate of the adults, thereby obtaining the result of drug resistance test.

[0020] (III) Beneficial effects

[0021] Compared with the prior art, the present invention provides a device for monitoring the insecticide resistance of citrus psyllid and a method for determining the insecticide resistance, which have the following beneficial effects:

[0022] 1. The citrus psyllid insecticide resistance monitoring device and insecticide resistance determination method, the adult insects in the dark box can reach the spray box through the transparent connecting tube, the first motor drives the shading plate to move in the moving groove through the reel and the first connecting rope to control the movement of the adult insects, the number of adult insects in the transparent connecting tube is detected by the infrared counter, the number of adult insects in the culture bottle is controlled, and the number of adult insects passing through at one time is guaranteed to be the number required in one culture bottle, thereby reducing the operator's workload and improving work efficiency. The citrus psyllid is sprayed with liquid medicine through the spraying component arranged in the spray box, so that the liquid medicine volatilization during manual spraying is avoided, and the operation safety is improved.

[0023] 2. The citrus psyllid pesticide resistance monitoring device and pesticide resistance determination method, when the first belt group rotates, drives the second belt group to rotate, so that the second belt group drives the second turntable to rotate, so that the second turntable drives the connecting frame and the connecting block to move along the limit frame through the second connecting rod, and when the connecting frame moves downward, it can drive the sealing plug to disconnect from the spray box, so that the adult insects leave the spray box and enter the culture bottle, avoiding the manual movement of the adults one by one, and improving the efficiency of adult movement.

[0024] 3. The citrus psyllid insecticide resistance monitoring device and insecticide resistance determination method, the operator can place the prepared culture bottle in the first placement box, and then the culture bottle can slide along the guide plate to the upper end of the third belt group, and be driven by the third belt group to the lower end of the spray box. After the adult insects are placed in the culture bottle, the culture bottle moves with the third belt group, and slides into the inner cavity of the second placement box after reaching the other end of the fixed frame, thereby further reducing the operator's workload and improving operating efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0026] Figure 2It is a schematic diagram of the top view of the structure of the second storage box of the present invention;

[0027] Figure 3 It is a partially cutaway three-dimensional structural schematic diagram of the fixing frame of the present invention;

[0028] Figure 4 This is a schematic diagram of the connection structure between the first belt group and the second motor of the present invention;

[0029] Figure 5 It is a partially cutaway three-dimensional structural schematic diagram of the spray box of the present invention;

[0030] Figure 6 It is a schematic diagram of the three-dimensional structure of the connection of the second connecting rope, the moving frame and the moving track of the present invention;

[0031] Figure 7 It is a partially cutaway three-dimensional structural schematic diagram of the connecting frame and the limiting frame of the present invention.

[0032] In the figure: 1, fixed frame; 2, dark box; 3, buffer frame; 4, first motor; 5, reel; 6, transparent connecting pipe; 7, spray box; 8, moving slot; 9, shading plate; 10, first connecting rope; 11, conveyor belt group; 12, conveyor plate; 13, second connecting rope; 14, moving frame; 15, moving track; 16, first connecting rod; 17, first turntable; 18, first belt group; 19, second motor; 20, third motor; 21, second belt group; 22, second turntable; 23, second connecting rod; 24, connecting frame; 25, limiting frame; 26, connecting block; 27, sealing plug; 28, culture bottle; 29, cap; 30, limiting spring; 31, mesh plate; 32, first placement box; 33, second placement box; 34, fourth motor; 35, third belt group; 36, limiting push block. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] As introduced in the background technology, there are deficiencies in the prior art. In order to solve the above technical problems, the present application proposes a citrus psyllid resistance monitoring device and a resistance determination method.

[0035] In a typical implementation of the present application, Figure 1-Figure 7As shown, a citrus psyllid pesticide resistance monitoring device comprises a fixed frame 1, a dark box 2 is fixedly connected to the top of the fixed frame 1, one end of the dark box 2 is fixedly connected to a transparent connecting tube 6, one end of the transparent connecting tube 6 is fixedly connected to a spray box 7, a moving groove 8 is opened at one end of the dark box 2, a shading plate 9 is slidably connected in the moving groove 8, a first connecting rope 10 is fixedly connected to the top of the shading plate 9, the first connecting rope 10 passes through one end of the dark box 2 and is wound with a reel 5, one end of the reel 5 is fixedly connected to a first motor 4, The first motor 4 is fixedly connected to the dark box 2, one end of the dark box 2 is fixedly connected to an infrared counter, the infrared counter is located at the upper end of the transparent connecting tube 6, a spraying assembly is arranged in the spraying box 7, the spraying assembly is used to spray liquid medicine on citrus psyllids to avoid the volatilization of liquid medicine when spraying liquid medicine manually, the spraying assembly is connected to the spraying box 7, when in use, the dark box 2 is fixedly connected to the top of the fixing frame 1, one end of the dark box 2 is fixedly connected to the transparent connecting tube 6, and one end of the transparent connecting tube 6 is fixedly connected to the spraying box 7, so that the fixing frame The position of the dark box 2 is fixed so that the adult insects in the dark box 2 can reach the spray box 7 through the transparent connecting tube 6. A moving groove 8 is opened at one end of the dark box 2. A shading plate 9 is slidably connected in the moving groove 8. A first connecting rope 10 is fixedly connected to the top of the shading plate 9. The first connecting rope 10 passes through one end of the dark box 2 and is wound with a reel 5. One end of the reel 5 is fixedly connected with a first motor 4, so that the first motor 4 can drive the shading plate 9 to move in the moving groove 8 through the reel 5 and the first connecting rope 10 to control the movement of the adult insects. An infrared counter is fixedly connected at one end of the dark box 2. The infrared counter is located at the upper end of the transparent connecting tube 6, so that the infrared counter can detect the number of adult insects in the transparent connecting tube 6, thereby controlling the number of adult insects in the culture bottle 28, ensuring that the number of adult insects passing through at one time is the number required in one culture bottle 28, reducing the workload of the operator, and improving work efficiency. The citrus psyllid is sprayed with liquid medicine through the spraying assembly arranged in the spray box 7, so as to avoid the volatilization of liquid medicine during manual spraying, and improve the safety of operation.

[0036] As a preferred implementation in this embodiment, refer to the attached Figure 4-Figure 7The medicine spraying assembly includes a conveyor belt group 11 rotatably connected to the medicine spraying box 7, a plurality of medicine spraying pipes and cold air pipes are fixedly connected to the top of the medicine spraying box 7, the conveyor belt group 11 is located at the lower ends of the medicine spraying pipes and the cold air pipes, one end of the conveyor belt group 11 is located below one end of the transparent connecting pipe 6, a conveyor plate 12 is placed below the other end of the conveyor belt group 11, one end of the conveyor plate 12 is rotatably connected to the medicine spraying box 7, one end of the medicine spraying box 7 is fixedly connected to a third motor 20, the output shaft of the third motor 20 penetrates into the medicine spraying box 7 and is fixedly sleeved with the conveyor belt group 11, the other end of the conveyor plate 12 is fixedly connected to a second connecting rope 13, the top end of the second connecting rope 13 penetrates to the outside of the medicine spraying box 7 and is fixedly connected to a moving frame 14, and the top end of the medicine spraying box 7 is fixedly connected to a moving track 15 The movable frame 14 is slidably connected with the movable track 15, the middle part of the movable frame 14 is rotatably connected with the first connecting rod 16, one end of the first connecting rod 16 is rotatably connected with the first turntable 17, the middle part of the first turntable 17 is fixedly connected with the first belt group 18, one end of the first belt group 18 is fixedly sleeved with the second motor 19, the side of the movable track 15 is T-shaped, the first turntable 17 is rotatably connected with the spray box 7, the second motor 19 is fixedly connected with the spray box 7, the transparent connecting pipe 6 is tilted, the gas temperature sprayed from the spray box 7 is below 8°C, the conveyor belt group 11 rotatably connected through the spray box 7, the output shaft of the third motor 20 penetrates into the spray box 7 and is fixedly sleeved with the conveyor belt group 11, so that the third motor 20 drives the conveyor belt group 11 to rotate, and the formed The insects move to the bottom of the spray port to ensure that the adult insects are sprayed with medicine. A number of spray pipes and cold air pipes are fixedly connected through the top of the spray box 7. The conveyor belt group 11 is located at the lower ends of the spray pipes and the cold air pipes. The transparent connecting pipe 6 is tilted. The temperature of the gas sprayed from the spray box 7 is below 8°C, so that the adult insects are first restricted by the low temperature to stop moving and then fall along the transparent connecting pipe 6 to the upper end of the conveyor belt group 11. Then, they are driven by the conveyor belt group 11 to the bottom of the spray pipe, so that the adult insects are restricted by the low temperature and will not move during spraying, thereby ensuring the spraying effect. One end of the conveyor belt group 11 is located below one end of the transparent connecting pipe 6, and a conveyor plate 12 is placed below the other end of the conveyor belt group 11, so that the adult insects at the upper end of the transparent connecting pipe 6 will fall to the upper end of the conveyor belt group 11. The conveyor belt group The adult insects at the upper end of 11 will be moved to the upper end of the conveying plate 12, so that the adult insects are restricted in movement by low temperature before spraying, and will be directly sent out after the spraying is completed, which ensures the spraying effect and improves the operation safety. A second connecting rope 13 is fixedly connected to the other end of the conveying plate 12, and the top of the second connecting rope 13 passes through the outside of the spray box 7 and is fixedly connected to a moving frame 14. The top of the spray box 7 is fixedly connected to a moving track 15, and the moving frame 14 is slidably connected to the moving track 15. The middle part of the moving frame 14 is rotatably connected to a first connecting rod 16, and one end of the first connecting rod 16 is rotatably connected to a first turntable 17. The middle part of the first turntable 17 is fixedly connected to a first belt group 18, and one end of the first belt group 18 is fixedly sleeved with a second motor 19.When the second motor 19 is started, the first rotating disc 17 is driven to rotate through the first belt group 18, so that the first rotating disc 17 drives the moving frame 14 to move along the moving track 15 through the first connecting rod 16, and the second connecting rope 13 is loosened, so that the conveying plate 12 rotates downward, so that the adult insects on the upper end of the conveying plate 12 slide out of the spray box 7, and the side of the moving track 15 is T-shaped, so that the connection between the moving frame 14 and the moving track 15 is stable.

[0037] As a preferred implementation in this embodiment, refer to the attached Figure 4 , Figure 5 and Figure 7One end of the first belt group 18 is rotatably connected to the second belt group 21, and the lower end of the second belt group 21 is fixedly connected to the second turntable 22, one end of the second turntable 22 is rotatably connected to the second connecting rod 23, one end of the second connecting rod 23 is rotatably connected to the connecting frame 24, and the middle part of the connecting frame 24 is fixedly connected to the connecting block 26, the lower end of the spray box 7 is fixedly connected to the limiting frame 25, the connecting block 26 is slidably connected in the limiting frame 25, and the bottom end of the connecting frame 24 is fixedly connected to the sealing plug 27, and the sealing plug 27 is fitted with the bottom end of the spray box 7. The connecting block 26 is a T block, and the sealing plug 27 is a hemispherical body. The spray box 7 includes a square box at the upper end and a tubular box at the lower end. The other end of the conveying plate 12 is located at the upper end of the tubular box of the spray box 7. The lower end of the fixed frame 1 is rotatably connected A third belt group 35 is connected, and a fourth motor 34 is fixedly connected to the lower end of the fixed frame 1. The output shaft of the fourth motor 34 passes through the fixed frame 1 and is fixedly sleeved with the third belt group 35. A culture bottle 28 is placed on the upper end of the third belt group 35. A cap 29 is threadedly connected to the upper end of the culture bottle 28. Both ends of the cap 29 are rotatably connected to a mesh plate 31. The lower end of the mesh plate 31 is fixedly connected to a limit spring 30. The lower end of the limit spring 30 is fixedly connected to the cap 29. Both mesh plates 31 are semicircular, and the two mesh plates 31 can form a full circle. The second belt group 21 is rotatably connected to one end of the first belt group 18. The lower end of the second belt group 21 is fixedly connected to the second turntable 22. One end of the second turntable 22 is rotatably connected to the second connecting rod 23. The second connecting rod 23 is rotatably connected to the second turntable 22. One end of the rod 23 is rotatably connected to a connecting frame 24, and a connecting block 26 is fixedly connected to the middle of the connecting frame 24. The lower end of the spray box 7 is fixedly connected to a limiting frame 25, and the connecting block 26 is slidably connected to the limiting frame 25, so that when the first belt group 18 rotates, it drives the second belt group 21 to rotate, so that the second belt group 21 drives the second turntable 22 to rotate, so that the second turntable 22 drives the connecting frame 24 and the connecting block 26 to move along the limiting frame 25 through the second connecting rod 23. A sealing plug 27 is fixedly connected to the bottom end of the connecting frame 24, and the sealing plug 27 is fitted with the bottom end of the spray box 7, so that when the connecting frame 24 moves downward, it can drive the sealing plug 27 to disconnect from the spray box 7, so that the adult insects leave the spray box 7 and enter the culture bottle 28 to avoid The adults are moved one by one manually to improve the efficiency of adult movement. The connection block 26 is a T block, so that the connection problem between the connection block 26 and the limit frame 25 is solved. The sealing plug 27 is a hemispherical body to prevent the adults from being trapped on the top of the sealing plug 27. The spray box 7 includes a square box at the upper end and a tubular box at the lower end. The other end of the conveying plate 12 is located at the upper end of the tubular box of the spray box 7, so that the adults can smoothly leave the spray box 7. The third belt group 35 is rotatably connected to the lower end of the fixed frame 1. The lower end of the fixed frame 1 is fixedly connected to the fourth motor 34. The output shaft of the fourth motor 34 passes through the fixed frame 1 and is fixedly connected to the third belt group 35. The upper end of the third belt group 35 is placed with a culture bottle 28, so that the fourth motor 34 drives the culture bottle 28 to move through the third belt group 35.The moving distance of the culture bottle 28 is controlled to ensure that the culture bottle 28 is located at the lower end of the spray box 7. The upper end of the culture bottle 28 is threadedly connected with a cap 29, so that the seedlings can be placed in the culture bottle 28. The two ends of the cap 29 are rotatably connected with a mesh plate 31. The lower end of the mesh plate 31 is fixedly connected to a limit spring 30. The lower end of the limit spring 30 is fixedly connected to the cap 29. The two mesh plates 31 are both semicircular, and the two mesh plates 31 can form a full circle. The connecting frame 24 is adapted to the mesh plate 31, so that when the connecting frame 24 moves downward, the mesh plate 31 can be pushed to open, so that the adult insects fall into the culture bottle 28. After the connecting frame 24 moves upward, the mesh plate 31 is driven by the limit spring 30 to return to the original position and close, so as to prevent the adult insects from leaving the inner cavity of the culture bottle 28, reduce the manual operation steps of the operator, and improve the operation efficiency.

[0038] As a preferred implementation in this embodiment, refer to the attached Figure 1-Figure 3 , the outer side of the third belt group 35 is fixedly connected with a plurality of groups of limiting push blocks 36, and the limiting push blocks 36 are adapted to the culture bottle 28. The two ends of the fixed frame 1 are respectively fixedly connected with the first placement box 32 and the second placement box 33. The bottom surfaces of the first placement box 32 and the second placement box 33 are both at a certain angle to the horizontal plane. The card slots opened at both ends of the fixed frame 1 are adapted to the culture bottle 28. The first placement box 32 and the second placement box 33 are fixedly connected with a guide plate on one side close to the fixed frame 1. The buffer frame 3 is fixedly connected with a buffer frame 3 on one side. The buffer frame 3 includes a baffle and a spring telescopic rod. The outer side of the third belt group 35 is fixedly connected with a plurality of groups of limiting push blocks 36. The limiting push blocks 36 are adapted to the culture bottle 28, so that the position of the culture bottle 28 is limited, so that the culture bottle 28 can be moved to the lower end of the spray box 7. The first placement box 32 and the second placement box 33 are respectively fixedly connected with the two ends of the fixed frame 1. The first placement box 32 and the second placement box 33 are fixedly connected The bottom surfaces of the placement boxes 33 are at a certain angle to the horizontal plane, and the card slots opened at both ends of the fixing frame 1 are adapted to the culture bottles 28. The first placement box 32 and the second placement box 33 are fixedly connected with guide plates on one side close to the fixing frame 1, so that the operator can place the prepared culture bottles 28 in the first placement box 32, and then the culture bottles 28 can slide along the guide plates to the upper end of the third belt group 35, and be driven by the third belt group 35 to the lower end of the spray box 7. After the culture bottles 28 are placed as adults, the culture bottles 28 move with the third belt group 35, and slide into the inner cavity of the second placement box 33 after reaching the other end of the fixing frame 1, further reducing the workload of the operator and improving the operating efficiency. A buffer frame 3 is fixedly connected with one side of the second placement box 33, and the buffer frame 3 includes a baffle and a spring telescopic rod, so that the buffer frame 3 can slow down the impact of the culture bottles 28, reduce the damage of the culture bottles 28 and the second placement box 33, and improve the service life of the device.

[0039] A method for determining the insecticide resistance of citrus psyllid, which is specifically applied to the above-mentioned citrus psyllid insecticide resistance monitoring device, comprises the following steps:

[0040] S1, placing the adult insects in the dark box 2, starting the first motor 4 to drive the shading plate 9 to move upward through the reel 5 and the first connecting rope 10, so that the adult insects are attracted by the light transmitted through the transparent connecting tube 6 and crawl into the transparent connecting tube 6, and at the same time the infrared counter counts, and when the set number is reached, the first motor 4 is driven to reverse, so that the shading plate 9 falls down under its own gravity, closing the passage between the dark box 2 and the transparent connecting tube 6;

[0041] S2, at this time, the spray box 7 starts to spray cold air pipes, so that the adult insects stop moving and slide down the transparent connecting pipe 6 to the upper end of the conveyor belt group 11, and then the conveyor belt group 11 drives the adult insects to move to the bottom of the spray pipe and start spraying according to the set spray;

[0042] S3. After spraying, the conveyor belt group 11 continues to rotate to drive the adult insects to the upper end of the conveying plate 12. Then, the second motor 19 drives the first belt group 18 and the second belt group 21 to rotate, so that the second connecting rope 13 drives the conveying plate 12 to rotate, so that the adult insects on the upper end of the conveying plate 12 fall along the conveying plate 12. At this time, the culture bottle 28 is located at the lower end of the spray box 7. The mesh plate 31 is opened under the push of the sealing plug 27, so that the falling adult insects fall into the culture bottle 28. Then, the mesh plate 31 is closed. The temperature in the culture bottle 28 is room temperature, and the adult insects will resume activity. Then, the culture bottle 28 is placed in an artificial climate box and re-fed for a certain number of days to check the survival rate of the adults, thereby obtaining the result of drug resistance detection, so that the counting method of adults is more accurate, ensuring that the number of adults in each culture bottle 28 is consistent with the set value, and also avoiding the escaping of the medicine during spraying to affect the safety of the operator, and eliminating the step of the operator moving the adults into the culture bottle 28 one by one, thereby improving the operation efficiency.

[0043] The working principle of the present invention is as follows: when in use, the adult insects in the dark box 2 can reach the spray box 7 through the transparent connecting tube 6, the first motor 4 drives the shading plate 9 to move in the moving groove 8 through the reel 5 and the first connecting rope 10 to control the movement of the adult insects, and the number of adult insects in the transparent connecting tube 6 is detected by the infrared counter to control the number of adult insects in the culture bottle 28 to ensure that the number of adult insects passing through at one time is the number required in one culture bottle 28, thereby reducing the workload of the operator and improving the work efficiency. The third motor 20 drives the conveyor belt group 11 to rotate, and moves the adult insects to the bottom of the spray port to ensure that the adult insects are sprayed with medicine. The adult insects are first restricted by low temperature to stop moving, and then fall along the transparent connecting tube 6 to the upper end of the conveyor belt group 11, and then are conveyed by the conveyor belt group 1 1 is driven to the bottom of the spraying pipe, so that the adult insects are restricted by the low temperature and cannot move when spraying, thereby ensuring the spraying effect. The adult insects at the upper end of the transparent connecting tube 6 will fall to the upper end of the conveyor belt group 11, and the adult insects at the upper end of the conveyor belt group 11 will be moved to the upper end of the conveyor plate 12, so that the adult insects are restricted by the low temperature to spray, and will be directly sent out after the spraying is completed, which ensures the spraying effect and improves the operation safety. When the second motor 19 is started, the first rotating disk 17 is driven to rotate through the first belt group 18, so that the first rotating disk 17 drives the moving frame 14 to move along the moving track 15 through the first connecting rod 16, and the second connecting rope 13 is loosened, so that the conveying plate 12 rotates downward, so that the adult insects at the upper end of the conveying plate 12 slide out of the spraying box 7.

[0044] When the first belt group 18 rotates, the second belt group 21 is driven to rotate, so that the second belt group 21 drives the second turntable 22 to rotate, so that the second turntable 22 drives the connecting frame 24 and the connecting block 26 to move along the limiting frame 25 through the second connecting rod 23, and when the connecting frame 24 moves downward, the sealing plug 27 can be driven to disconnect from the spray box 7, so that the adult insects leave the spray box 7 and enter the culture bottle 28, avoiding manual movement of the adult insects one by one, and improving the movement efficiency of the adult insects. The fourth motor 34 drives the culture bottle 28 to move through the third belt group 35, controls the movement distance of the culture bottle 28, and ensures that the culture bottle 28 is located at the lower end of the spray box 7. When the connecting frame 24 moves downward, the mesh plate 31 can be pushed to open, so that the adult insects fall into the culture bottle 28. After the connecting frame 24 moves upward, the mesh plate 31 is driven by the limiting spring 30 to return to the original position and close, avoiding the adult insects from leaving the inner cavity of the culture bottle 28, reducing the manual operation steps of the operator, and improving the operation efficiency.

[0045] The operator can place the prepared culture bottle 28 in the first placement box 32, and then the culture bottle 28 can slide along the guide plate to the upper end of the third belt group 35, and be driven by the third belt group 35 to the lower end of the spray box 7. After the culture bottle 28 is placed with the adult insects, the culture bottle 28 moves with the third belt group 35, and slides into the inner cavity of the second placement box 33 after reaching the other end of the fixed frame 1, further reducing the operator's workload and improving operating efficiency. The buffer frame 3 can slow down the impact of the culture bottle 28, reduce the damage of the culture bottle 28 and the second placement box 33, and improve the service life of the device.

[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for monitoring citrus psyllid resistance to pesticides, characterized in that: The invention comprises a fixing frame (1), the top of the fixing frame (1) is fixedly connected to a dark box (2), one end of the dark box (2) is fixedly connected to a transparent connecting tube (6), one end of the transparent connecting tube (6) is fixedly connected to a spray box (7), one end of the dark box (2) is provided with a moving groove (8), a shading plate (9) is slidably connected in the moving groove (8), the top of the shading plate (9) is fixedly connected to a first connecting rope (10), and the first connecting rope (10) passes through the dark box (2). A reel (5) is wound around one end of the reel (5), a first motor (4) is fixedly connected to one end of the dark box (2), an infrared counter is fixedly connected to one end of the dark box (2), the infrared counter is located at the upper end of the transparent connecting tube (6), a spraying assembly is arranged in the spraying box (7), the spraying assembly is used to spray liquid medicine on citrus psyllids to prevent liquid medicine from volatilizing when spraying liquid medicine manually, and the spraying assembly is connected to the spraying box (7).

2. A citrus psyllid insecticide resistance monitoring device according to claim 1, characterized in that: The spraying assembly comprises a conveyor belt group (11) rotatably connected to the spraying box (7); a plurality of spraying pipes and cold air pipes are fixedly connected to the top of the spraying box (7); the conveyor belt group (11) is located at the lower end of the spraying pipes and the cold air pipes; one end of the conveyor belt group (11) is located below one end of the transparent connecting pipe (6); a conveyor plate (12) is placed below the other end of the conveyor belt group (11); one end of the conveyor plate (12) is rotatably connected to the spraying box (7); one end of the spraying box (7) is fixedly connected to a third motor (20); an output shaft of the third motor (20) passes through the spraying box (7) and is fixedly connected to the conveyor belt group (11).

3. A citrus psyllid insecticide resistance monitoring device according to claim 2, characterized in that: The other end of the conveying plate (12) is fixedly connected to a second connecting rope (13), the top end of the second connecting rope (13) passes through the outside of the spray box (7) and is fixedly connected to a moving frame (14), the top end of the spray box (7) is fixedly connected to a moving track (15), the moving frame (14) is slidably connected to the moving track (15), the middle part of the moving frame (14) is rotatably connected to a first connecting rod (16), one end of the first connecting rod (16) is rotatably connected to a first turntable (17), the middle part of the first turntable (17) is fixedly connected to a first belt group (18), and one end of the first belt group (18) is fixedly sleeved with a second motor (19).

4. A device for monitoring insecticide resistance of citrus psyllid according to claim 3, characterized in that: One end of the first belt group (18) is rotatably connected to the second belt group (21), the lower end of the second belt group (21) is fixedly connected to the second turntable (22), one end of the second turntable (22) is rotatably connected to the second connecting rod (23), one end of the second connecting rod (23) is rotatably connected to the connecting frame (24), the middle part of the connecting frame (24) is fixedly connected to a connecting block (26), the lower end of the spray box (7) is fixedly connected to a limiting frame (25), the connecting block (26) is slidably connected in the limiting frame (25), the bottom end of the connecting frame (24) is fixedly connected to a sealing plug (27), and the sealing plug (27) is in contact with the bottom end of the spray box (7).

5. The device for monitoring insecticide resistance of citrus psyllid according to claim 3, characterized in that: The side surface of the movable track (15) is T-shaped, the first turntable (17) is rotatably connected to the spray box (7), and the second motor (19) is fixedly connected to the spray box (7).

6. The device for monitoring insecticide resistance of citrus psyllid according to claim 4, characterized in that: The connecting block (26) is a T-block, the sealing plug (27) is a hemispherical body, the spray box (7) comprises a square box at the upper end and a tubular box at the lower end, the other end of the conveying plate (12) is located at the upper end of the tubular box of the spray box (7), the transparent connecting pipe (6) is arranged at an angle, and the temperature of the gas sprayed from the spray box (7) is below 8°C.

7. The device for monitoring insecticide resistance of citrus psyllid according to claim 1, characterized in that: The lower end of the fixed frame (1) is rotatably connected to a third belt group (35), and the lower end of the fixed frame (1) is fixedly connected to a fourth motor (34), the output shaft of the fourth motor (34) passes through the fixed frame (1) and is fixedly sleeved with the third belt group (35), a culture bottle (28) is placed on the upper end of the third belt group (35), the upper end of the culture bottle (28) is threadedly connected to a cap (29), both ends of the cap (29) are rotatably connected to a mesh plate (31), the lower end of the mesh plate (31) is fixedly connected to a limit spring (30), the lower end of the limit spring (30) is fixedly connected to the cap (29), and the two mesh plates (31) are both semicircular.

8. The device for monitoring insecticide resistance of citrus psyllid according to claim 7, characterized in that: The two mesh plates (31) can form a full circle.

9. The device for monitoring insecticide resistance of citrus psyllid according to claim 7, characterized in that: The outer side of the third belt group (35) is fixedly connected with a plurality of groups of limit push blocks (36), and the limit push blocks (36) are adapted to the culture bottles (28). The two ends of the fixed frame (1) are respectively fixedly connected with a first placement box (32) and a second placement box (33), and the bottom surfaces of the first placement box (32) and the second placement box (33) are both at a certain angle to the horizontal plane. The hole opened in the middle of the fixed frame (1) is adapted to the culture bottles (28). The first placement box (32) and the second placement box (33) are both fixedly connected with a guide plate on one side close to the fixed frame (1), and the second placement box (33) is fixedly connected with a buffer frame (3) on one side, and the buffer frame (3) includes a baffle and a spring telescopic rod.

10. A method for determining insecticide resistance of citrus psyllid, specifically applied to a citrus psyllid insecticide resistance monitoring device as claimed in any one of claims 1 to 9, characterized in that: The steps include: S1, placing the adult insects in the dark box (2), starting the first motor (4) to drive the shading plate (9) to move upwards through the reel (5) and the first connecting rope (10), so that the adult insects are attracted by the light transmitted through the transparent connecting tube (6) and crawl into the transparent connecting tube (6), and at the same time the infrared counter counts, and when the set number is reached, the first motor (4) is driven to reverse, so that the shading plate (9) falls down under its own gravity, and the passage between the dark box (2) and the transparent connecting tube (6) is closed; S2, at this time, the spray box (7) starts to spray cold air pipes, so that the adult insects stop moving and then slide down along the transparent connecting pipe (6) to the upper end of the conveyor belt group (11), and then the conveyor belt group (11) drives the adult insects to move to the bottom of the spray pipe and start spraying according to the set spraying method; S3, after the spraying is finished, the conveyor belt group (11) continues to rotate and drives the adult insects to the upper end of the conveying plate (12), and then the second motor (19) drives the first belt group (18) and the second belt group (21) to rotate, so that the second connecting rope (13) drives the conveying plate (12) to rotate, so that the adult insects on the upper end of the conveying plate (12) fall along the conveying plate (12), at this time, the culture bottle (28) is located at the lower end of the spray box (7), and the mesh plate (31) is opened under the push of the sealing plug (27), so that the falling adult insects fall into the culture bottle (28), and then the mesh plate (31) is closed, the temperature in the culture bottle (28) is room temperature, and the adult insects will resume activity, and then the culture bottle (28) is placed in an artificial climate box for re-feeding for a certain number of days, and the survival rate of the adult insects can be checked, thereby obtaining the result of the drug resistance test.

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