Microbial fermentation type tifrog feed additive and application

Abstract

The invention relates to a microbial fermentation type Rana tigrina feed additive and application, and belongs to the technical field of feed additives, the additive comprises the following raw materials: broad bean straw, navel orange residues, paper mulberry leaves, cassava leaves, soybean oil, grass carp oil, earthworm powder, lactobacillus acidophilus, aspergillus oryzae and bacillus subtilis. The broad bean straw, the navel orange residues, the paper mulberry leaves and the cassava leaves are combined to complement the deficiencies, high nutritional value is provided for the Rana tigrina, and the growth speed of the Rana tigrina is increased; on the basis, compound bacteria formed by compounding lactobacillus acidophilus, aspergillus oryzae and bacillus subtilis are also selected for co-fermentation, so that the plant raw materials can be effectively assisted to release and utilize effective components of the plant raw materials, the nutritional value of the additive is further improved, and the growth promotion effect is exerted to a great extent; in addition, the soybean oil, the grass carp oil and the earthworm powder are also added, so that the prepared additive has an extremely good food calling effect.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and in particular relates to a microbial fermentation type Thai frog feed additive and application thereof. Background Art

[0002] In today's breeding industry, Thai frog breeding occupies an important position. With people's continuous pursuit of the quality and yield of breeding Thai frogs, the demand for high-quality feed additives has become increasingly urgent, and traditional Thai frog feed additives have gradually revealed some limitations. On the one hand, although traditional Thai frog feed additives can meet the nutritional needs of Thai frogs to a certain extent in terms of growth promotion, it is difficult to fully stimulate the growth potential, digestion and absorption rate of Thai frogs. On the other hand, due to the particularity of the visual physiology of Thai frogs, they are visually insensitive to static objects and cannot fully stimulate the appetite of Thai frogs. Therefore, in breeding, the phenomenon of feed not being eaten often occurs, resulting in feed waste and environmental pollution. At the same time, as people pay more and more attention to food safety and environmental protection, the requirements for Thai frog feed additives have become more stringent. Traditional chemically synthesized additives may cause residual problems and cause potential harm to human health and the environment. Microbial fermentation additives, with their natural, safe and environmentally friendly characteristics, have gradually become a research hotspot in the field of Thai frog breeding.

[0003] Therefore, it is of great practical significance to develop a microbial fermented Thai frog feed additive with excellent growth-promoting and feeding effects. At the same time, through scientific formula design and fermentation technology, the feeding properties of feed additives can be enhanced, the appetite of Thai frogs can be stimulated, the growth-promoting effect can be improved, and higher economic benefits and sustainable development momentum can be brought to the Thai frog breeding industry. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial fermentation type Thai frog feed additive and application, which solves the problem that the feed additives in the prior art have poor growth-promoting and feeding-inducing effects.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A microbial fermentation type Thai frog feed additive, the additive comprising the following raw materials in parts by weight:

[0007]

[0008] Broad bean straw is rich in nutrients such as amino acids, proteins and trace elements; navel orange pulp is rich in soluble carbohydrates, vitamin C, carotene and various minerals; paper mulberry leaves are rich in protein, calcium, phosphorus and various vitamins; cassava leaves are rich in nutrients such as fat, protein and cellulose.

[0009] As a preferred technical solution of the present invention, the additive further comprises 0.4-0.5 parts by weight of composite bacteria.

[0010] Furthermore, the composite bacteria include Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis.

[0011] Furthermore, the mass ratio of the Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis is 1:0.8-0.9:1.1-1.2.

[0012] Furthermore, the viable count of Lactobacillus acidophilus is 3.00×10 6 CFU / g, and the number of viable bacteria of Aspergillus oryzae was 6×10 7 CFU / g, the number of viable bacteria of Bacillus subtilis is 2.00×10 7 CFU / g.

[0013] Lactobacillus acidophilus can grow and produce a large amount of lactic acid in the early stage of fermentation, quickly reduce the pH of the system and inhibit the growth and reproduction of harmful bacteria, thereby improving the quality of the finished additive; Aspergillus oryzae can produce abundant proteases, cellulases and amylases during the fermentation process, which can break down proteins into small molecular peptides and amino acids, and degrade difficult-to-absorb substances such as crude fiber and phytic acid in the system, which is beneficial to the effectiveness of the raw materials in the system; Bacillus subtilis can quickly consume oxygen and increase the abundance of Lactobacillus acidophilus, and at the same time can secrete a variety of enzymes to degrade xylan, pectin and other plant raw materials in the system into soluble carbohydrates, thereby providing nutritional value for the fermentation substrate.

[0014] As a preferred technical solution of the present invention, the preparation method of the additive specifically comprises the following steps:

[0015] Broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are dried, crushed and sieved, and the sieved broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are mixed evenly to obtain a mixture A, deionized water and composite bacteria are added to the mixture A and stirred evenly, the air is expelled and sealed, fermented to obtain a fermented material, earthworm powder is added to the fermented material and mixed evenly to obtain a mixture B, soybean oil and grass carp oil are mixed evenly and sprayed on the surface of the mixture B while stirring to obtain a finished additive product.

[0016] Furthermore, the screening is through a 80-100 mesh sieve; and the fermentation is performed at room temperature for 28-30 days.

[0017] Furthermore, the dosage ratio of the mixture A to deionized water is 1g:20-25mL.

[0018] As a preferred technical solution of the present invention, the additive is used as one of the raw materials and added to Thai frog feed.

[0019] Furthermore, the additive is added in an amount of 1.5-2% of the total mass of the Thai frog feed.

[0020] Beneficial effects of the present invention:

[0021] (1) The present invention adds composite bacteria to co-ferment broad bean straw, navel orange residue, paper mulberry leaves and cassava leaves, thereby constructing a dual effect of the composite bacteria promoting the release and utilization of effective components of plant raw materials, and the plant raw materials providing sufficient substrate for the reproduction and fermentation of composite microorganisms, thereby solving the problem that these raw materials are not conducive to animal digestion and absorption when freshly fed, and further improving the utilization rate of effective components of plant raw materials in the additive, so that the prepared additive has excellent nutritional components and has a good promoting effect on the growth of Thai frogs.

[0022] (2) The present invention combines broad bean straw, navel orange residue, paper mulberry leaves and cassava leaves to complement each other, provide higher nutritional value for Thai frogs, accelerate their growth rate, and have higher feeding value; on this basis, a composite bacteria composed of Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis is selected for co-fermentation, which can effectively assist plant raw materials in releasing and utilizing their effective ingredients, thereby further improving the nutritional value of the additive and maximizing the growth-promoting effect of the additive.

[0023] (3) The present invention adds soybean oil, grass carp oil and earthworm powder, which increases the food attractant effect of the finished additive. Specifically, the octadecene series highly unsaturated fatty acids rich in soybean oil and the multiple unsaturated fatty acids and strong fishy smell rich in grass carp oil can play a good food attractant effect; and the earthworm powder can emit a special smell that is a strong signal to the Thai frog, stimulating the olfactory organ of the Thai frog, thereby further strengthening the food attractant effect of the additive. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The viable count of Lactobacillus acidophilus used in the present invention is 3.00×10 6 CFU / g, and the number of viable bacteria of Aspergillus oryzae was 6×10 7 CFU / g, the number of viable bacteria of Bacillus subtilis is 2.00×10 7 CFU / g; the above, no further elaboration will be given later.

[0026] Example 1

[0027] A microbial fermentation type Thai frog feed additive, the additive comprising the following raw materials in parts by weight:

[0028]

[0029] The composite bacteria comprises Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis in a mass ratio of 1:0.85:1.1;

[0030] The preparation method of the additive specifically comprises the following steps:

[0031] Broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are dried, crushed and passed through a 100-mesh sieve, the sieved broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are mixed uniformly to obtain a mixture A, deionized water and composite bacteria are added to the mixture A and stirred uniformly, air is exhausted and sealed, and fermented at room temperature for 28 days to obtain a fermented material, earthworm powder is added to the fermented material and mixed uniformly to obtain a mixture B, soybean oil and grass carp oil are mixed uniformly and sprayed on the surface of the mixture B while stirring to obtain a finished additive;

[0032] The dosage ratio of the mixed material A to deionized water is 1 g:22.5 mL.

[0033] Example 2

[0034] A microbial fermentation type Thai frog feed additive, the additive comprising the following raw materials in parts by weight:

[0035]

[0036] The composite bacteria comprises Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis in a mass ratio of 1:0.98:1.15;

[0037] The preparation method of the additive specifically comprises the following steps:

[0038] Broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are dried, crushed and passed through an 80-mesh sieve, the sieved broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are mixed uniformly to obtain a mixture A, deionized water and composite bacteria are added to the mixture A and stirred uniformly, air is exhausted and sealed, and fermented at room temperature for 29 days to obtain a fermented material, earthworm powder is added to the fermented material and mixed uniformly to obtain a mixture B, soybean oil and grass carp oil are mixed uniformly and sprayed on the surface of the mixture B while stirring to obtain a finished additive product;

[0039] The dosage ratio of the mixed material A to deionized water is 1 g:20 mL.

[0040] Example 3

[0041] A microbial fermentation type Thai frog feed additive, the additive comprising the following raw materials in parts by weight:

[0042]

[0043] The composite bacteria comprises Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis in a mass ratio of 1:0.8:1.2;

[0044] The preparation method of the additive specifically comprises the following steps:

[0045] Broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are dried, crushed and passed through a 90-mesh sieve, the sieved broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are mixed uniformly to obtain a mixture A, deionized water and composite bacteria are added to the mixture A and stirred uniformly, air is exhausted and sealed, and fermented at room temperature for 30 days to obtain a fermented material, earthworm powder is added to the fermented material and mixed uniformly to obtain a mixture B, soybean oil and grass carp oil are mixed uniformly and sprayed on the surface of the mixture B while stirring to obtain a finished additive product;

[0046] The dosage ratio of the mixed material A to deionized water is 1 g:25 mL.

[0047] Comparative Examples 1-4

[0048] Compared with Example 3, the difference is that the addition amounts of broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves in Comparative Examples 1-4 are as shown in Table 1, and the other operating steps and parameters remain unchanged.

[0049] Table 1

[0050]

[0051]

[0052] Comparative Example 5

[0053] Compared with Example 3, the difference is that in Comparative Example 5, Lactobacillus acidophilus is not added, that is, the composite bacteria includes Aspergillus oryzae and Bacillus subtilis in a mass ratio of 1.3:1.7, and the other operating steps and parameters remain unchanged.

[0054] Comparative Example 6

[0055] Compared with Example 3, the difference is that in Comparative Example 6, Aspergillus oryzae is not added, that is, the composite bacteria includes Lactobacillus acidophilus and Bacillus subtilis in a mass ratio of 1.4:1.6, and the other operating steps and parameters remain unchanged.

[0056] Comparative Example 7

[0057] Compared with Example 3, the difference is that in Comparative Example 7, Bacillus subtilis is not added, that is, the composite bacteria includes Lactobacillus acidophilus and Aspergillus oryzae in a mass ratio of 1.6:1.4, and the other operating steps and parameters remain unchanged.

[0058] Comparative Example 8

[0059] Compared with Example 3, the difference is that in Example 8, soybean oil is not added, grass carp oil is 5 parts by weight, earthworm powder is 17 parts by weight, and other operating steps and parameters remain unchanged.

[0060] Comparative Example 9

[0061] Compared with Example 3, the difference is that in Example 9, no grass carp oil is added, while soybean oil is 5.5 parts by weight and earthworm powder is 16.5 parts by weight, and the other operating steps and parameters remain unchanged.

[0062] Comparative Example 10

[0063] Compared with Example 3, the difference is that in Example 10, no earthworm powder is added, while the soybean oil is 11.5 parts by weight and the grass carp oil is 10.5 parts by weight, and the other operating steps and parameters remain unchanged.

[0064] Test Example 1

[0065] The additives obtained in Examples 1-3 and Comparative Examples 1-10 were added to the same frog feed matrix (including corn, soybean meal, cottonseed meal, rice bran, sodium glutamate, and lysine in a mass ratio of 6:2:1:0.8:0.02:0.05) at 1.5% of the total mass of the frog feed to obtain a finished frog feed. The frogs weighing 35.82-38.69g purchased from the same batch were randomly distributed to 13 disinfected cubic frog ponds with a side length of 1.5m, with 120 frogs in each pond; after the frogs entered the pond, they were fed with the same complete feed for 7 days of domestication from the second day, and the feeding amount was based on the frogs eating it within 1 hour. After the domestication was completed, the finished frog feeds obtained in Examples 1-3 and Comparative Examples 1-10 were fed in 13 frog ponds, and were fed at regular intervals twice a day (8:00 and 18:00) until they were full. During the test period, the water was changed once every week, the frog pond was cleaned once, and disinfected once every half a month. The breeding cycle was 30 days, and the death, average body weight, initial total body weight, final total body weight, and total mass of ingested feed of each pond of Thai frogs fed with the finished Thai frog feed prepared in Examples 1-3 and Comparative Examples 1-10 were recorded during the breeding period;

[0066] Survival rate (%) = number of frogs remaining after the end of breeding / number of frogs at the beginning of breeding*100%;

[0067] Food intake rate (% / d) = 100 × W D / (W t / 2+W0 / 2) / t;

[0068] W D : Total mass of ingested feed (g), W t : total mass of Thai frog after breeding (g), W0: total mass of Thai frog at the beginning of breeding (g); t: number of days of feeding;

[0069] The results are shown in Tables 2 and 3.

[0070] Table 2

[0071]

[0072]

[0073] As can be seen from Table 2, the additive prepared by the present invention has a good promoting effect on the growth of Thai frog. Specifically, the present invention combines broad bean straw, navel orange residue, paper mulberry leaves and cassava leaves to complement each other, providing higher nutritional value for Thai frog and accelerating its growth rate; on this basis, a composite bacteria composed of Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis is selected for co-fermentation, which can effectively assist plant raw materials to release and utilize their effective ingredients, thereby further improving the nutritional value of the additive and greatly exerting the growth-promoting effect.

[0074] Table 3

[0075]

[0076] As can be seen from Table 3, the additive prepared by the present invention has an excellent food attractant effect. Specifically, the present invention adds soybean oil, grass carp oil and earthworm powder, and the octadecene series highly unsaturated fatty acids rich in soybean oil and the multiple unsaturated fatty acids and strong fishy smell rich in grass carp oil can play a good food attractant effect; and the earthworm powder can emit a special smell that is a strong signal to Thai frogs, stimulating the olfactory organ of Thai frogs, thereby further strengthening the food attractant effect of the additive.

[0077] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0078] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A microbial fermentation type Thai frog feed additive, characterized in that: The additives include the following raw materials in parts by weight:

2. A microbial fermentation type Thai frog feed additive according to claim 1, characterized in that: The additive also includes 0.4-0.5 parts by weight of composite bacteria.

3. A microbial fermentation type Thai frog feed additive according to claim 2, characterized in that, The composite bacteria include Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis.

4. A microbial fermentation type Thai frog feed additive according to claim 3, characterized in that: The mass ratio of the Lactobacillus acidophilus, Aspergillus oryzae and Bacillus subtilis is 1:0.8-0.9:1.1-1.

2.

5. A microbial fermentation type Thai frog feed additive according to claim 3, characterized in that: The viable count of Lactobacillus acidophilus is 3.00×10 6 CFU / g, and the number of viable bacteria of Aspergillus oryzae was 6×10 7 CFU / g, the number of viable bacteria of Bacillus subtilis is 2.00×10 7 CFU / g.

6. The method for preparing the microbial fermentation type Thai frog feed additive according to claim 3, characterized in that: The specific steps include: Broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are dried, crushed and sieved, and the sieved broad bean straw, navel orange pulp, paper mulberry leaves and cassava leaves are mixed evenly to obtain a mixture A, deionized water and composite bacteria are added to the mixture A and stirred evenly, the air is expelled and sealed, fermented to obtain a fermented material, earthworm powder is added to the fermented material and mixed evenly to obtain a mixture B, soybean oil and grass carp oil are mixed evenly and sprayed on the surface of the mixture B while stirring to obtain a finished additive product.

7. The microbial fermentation type Thai frog feed additive according to claim 6, characterized in that: The screening is through a 80-100 mesh sieve; and the fermentation is carried out at room temperature for 28-30 days.

8. The microbial fermentation type Thai frog feed additive according to claim 6, characterized in that: The dosage ratio of the mixed material A to deionized water is 1 g:20-25 mL.

9. An application of the microbial fermentation type Thai frog feed additive as claimed in any one of claims 3 to 8, characterized in that: Added as one of the raw materials to Thai frog feed.

10. The use of the microbial fermentation type Thai frog feed additive according to claim 9, characterized in that: The additive is added in an amount of 1.5-2% of the total mass of the Thai frog feed.

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