Eye cream containing composition with efficacy of improving eye skin problems and preparation method of eye cream

Abstract

The invention discloses eye cream containing a composition capable of improving eye skin problems and a preparation method of the eye cream, and belongs to the technical field of cosmetics. The eye cream provided by the invention contains a repairing composition, and the repairing composition is prepared from the following raw materials: an acer rubrum extract, a zingiber zingiberensis extract, a dendrobium officinale extract, a North American arbor pine bark extract and an epilobium fragile extract. The repairing composition provided by the invention has the effects of resisting aging, fading wrinkles and improving the migration rate of skin cells, and the whole repairing composition is mild and non-irritant; when being applied to the eye cream, the repairing composition can effectively resist aging, lighten wrinkles, improve skin elasticity and increase the water content of cuticle. In addition, the composition provided by the invention is natural in component source, simple in preparation method and suitable for actual production.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an eye cream containing an effective composition for improving eye skin problems and a preparation method thereof. Background Art

[0002] Aging is a physiological and functional degeneration caused by various genetic and environmental factors, which ultimately affects the internal environment of biological organisms, destroys the balance of the internal microenvironment and increases susceptibility to diseases. Among them, skin aging is inevitable, and its specific manifestations include: tissue atrophy, fragility, repair defects, immune deficiency and susceptibility to infection. Skin aging can lead to a series of adverse consequences, such as water loss, dry or peeling skin, pigmentation, wrinkles, etc. The aging level is getting higher and higher, and the problem of skin aging is getting more and more serious, making people's demand for skin anti-aging more and more intense. The aging of the body will lead to a decrease in the number of stem cells and a decrease in cell activity. Although skin aging is inevitable, the speed of aging can be slowed down to a certain extent through technical intervention. Flavonoids and polyphenols in plant extracts have strong antioxidant properties and can effectively delay aging. In addition, the acid substances and biological enzymes therein can also effectively inhibit the activity of elastase and promote the production of collagen, which has a certain effect of delaying aging. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and thus provide a repair composition that can effectively resist aging, reduce wrinkles, enhance skin elasticity, increase the moisture content of the stratum corneum, whiten the skin and is mild and non-irritating.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides a repair composition, comprising the following components in parts by weight:

[0006] Red maple extract: 0.1-6 parts;

[0007] Ginger extract: 0.1-5 parts;

[0008] Dendrobium officinale extract: 0.5-25 parts;

[0009] North American pine bark extract: 0.1-5 parts;

[0010] Fleischer's Epilobium extract: 0.1-6 parts.

[0011] Preferably: the composition comprises the following components by weight

[0012] Red maple extract: 1-3 parts;

[0013] Ginger extract: 0.5-3 parts;

[0014] Dendrobium officinale extract: 5-15 parts;

[0015] North American pine bark extract: 0.5-2 parts;

[0016] Fleischer's Epilobium Extract: 1-3 parts.

[0017] in:

[0018] Red maple extract has excellent DPPH free radical scavenging ability and is a natural antioxidant. Antioxidants can neutralize free radicals and reduce oxidative stress, thereby protecting the skin from damage caused by environmental factors such as ultraviolet rays, and helping to fight wrinkles and whiten the skin.

[0019] Ginger extract can inhibit elastase and promote collagen production; it can also inhibit the activity of tyrosinase and has a whitening effect.

[0020] Dendrobium officinale extract has anti-wrinkle activity and can inhibit ultraviolet B (UVB)-induced skin photoaging by regulating the expression of matrix metalloproteinases (MMPs) and the production of type I collagen.

[0021] North American pine bark extract and its active ingredient Trans-Communic acid can inhibit UVB-induced MMP-1 expression, reduce collagen degradation by inhibiting the targeted PI3K pathway, and thus inhibit the formation of wrinkles.

[0022] Fleischer's epilobium extract has significant antioxidant, anti-inflammatory and skin microbiome regulation effects due to its rich flavonoids quercitrin, isoquercitrin, myricitrin and isomyricitrin and tannins ellagitannin and oenothelin B.

[0023] This study found that the combined use of the above components can synergistically enhance the effects of improving skin elasticity, reducing skin wrinkles, increasing skin cell mobility, whitening, and increasing cell water content.

[0024] As a preferred embodiment of the repair composition of the present invention, the repair composition comprises the following components in parts by weight:

[0025] Red maple extract: 1-2 parts;

[0026] Ginger extract: 0.5-1 part;

[0027] Dendrobium officinale extract: 10-15 parts;

[0028] North American pine bark extract: 0.5-1 part;

[0029] Fleischer's Epilobium Extract: 1-2 parts.

[0030] As the most preferred embodiment of the repair composition of the present invention, the repair composition comprises the following components by weight:

[0031] Red maple extract: 2 parts;

[0032] Red Ginger Extract: 1 part;

[0033] Dendrobium officinale extract: 10 parts;

[0034] North American pine bark extract: 1 part;

[0035] Fleischer's Epilobium Extract: 2 parts.

[0036] The present invention has found that the mass fraction of each component in the repair composition will affect the performance of the product. When the mass fraction of the components is further selected to be within the above range (including the endpoint value), the obtained product can more significantly improve the skin's elasticity, anti-oxidation, skin cell mobility and other effects.

[0037] In a second aspect, the present invention provides an eye cream, wherein the eye cream contains the repair composition described in the first aspect of the present invention.

[0038] As a preferred embodiment of the eye cream of the present invention, the eye cream is composed of the following components in percentage by mass:

[0039] Repair composition 1-10wt%;

[0040] Surfactant: 0.1-5wt%;

[0041] Humectant: 1-15wt%;

[0042] Preservative: 0.1-1.5wt%;

[0043] pH regulator: 0.01-0.3wt%;

[0044] Thickener: 0.1-1.5wt%;

[0045] Grease: 1-20wt%;

[0046] The balance was deionized water.

[0047] The surfactant is one or more of sucrose polystearate, glyceryl stearate citrate, and PEG-100 stearate;

[0048] The moisturizing agent is one or more of sodium hyaluronate, butylene glycol, and glyceryl polyether-26;

[0049] The preservative is one or more of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol;

[0050] The pH regulator is arginine;

[0051] The thickener is one or more of carbomer and / or ammonium acryloyldimethyltaurate / VP copolymer;

[0052] The oil is one or more of cetearyl alcohol, dimethicone, and caprylic / capric triglyceride.

[0053] In a third aspect of the present invention, the present invention provides a method for preparing an eye cream, the preparation method specifically comprising the following steps:

[0054] S1: Evenly mix the red maple extract, the red ginger extract, the dendrobium candidum extract, the North American pine bark extract, and the Fleischer's epilobium extract to obtain a repairing composition;

[0055] S2: Add the moisturizer and thickener into the emulsifying pot, heat to 70-90°C, stir evenly, and obtain phase A;

[0056] S3: Add surfactant and oil into an oil pan, heat to 70-90°C, stir and add into phase A to obtain phase B;

[0057] S4: Add the pH adjuster, preservative and repair composition into phase B, cool to room temperature, stir evenly to obtain eye cream.

[0058] Compared with the prior art, the present invention has the following beneficial effects:

[0059] The present invention provides a repairing composition, in which five extracts can cooperate with each other and synergistically enhance the effects, thereby achieving anti-aging effects, improving the mobility of skin cells, and being overall mild and non-irritating.

[0060] Specifically, the present invention innovatively selects specific mass fractions of Acer truncatum extract, Ginger extract, Dendrobium candidum extract, Pinus bark extract, and Epilobium fleischerii extract in combination to prepare a repair composition, in which the components can cooperate with each other and synergize to solve the problems of skin sagging, aging, dark complexion, increased wrinkles, etc. caused by external environmental factors. Specifically, the repair composition provided by the present invention can effectively improve skin elasticity, reduce skin wrinkles, increase skin cell mobility, whiten, and increase cell water content.

[0061] Specifically, in the composition used in the present invention, the red maple extract, the red ginger extract, the dendrobium candidum extract, the North American pine bark extract and the Fleischer's epilobium extract all have good antioxidant activity. They can jointly protect skin cells from damage caused by external factors such as ultraviolet rays by scavenging free radicals and reducing oxidative stress, thereby playing a synergistic role in anti-wrinkle and whitening.

[0062] Ginger extract, Dendrobium officinale extract and Pinus juncea bark extract can inhibit the expression of matrix metalloproteinases (MMPs). By jointly inhibiting the expression of MMPs, they reduce the degradation of collagen and thus exert a synergistic effect in anti-wrinkle.

[0063] Dendrobium officinale extract and Ginger extract can promote the synthesis of collagen, increase the collagen content in the skin, improve skin elasticity, and reduce wrinkles, thus playing a synergistic role in anti-wrinkle.

[0064] Both red maple extract and red ginger extract have the effect of inhibiting tyrosinase activity, which is the key enzyme in the production of melanin. By jointly inhibiting tyrosinase activity, the production of melanin is reduced, thus exerting a synergistic effect in whitening.

[0065] Dendrobium officinale extract can protect collagen homeostasis and reduce skin damage by regulating the TGFβ / Smad pathway. This regulatory effect may work synergistically with other extracts to improve skin conditions and enhance anti-wrinkle and whitening effects.

[0066] In addition, the repair composition provided by the present invention is mild and non-irritating as a whole, has a wide range of applicable objects, and has a simple preparation method, and is suitable for actual production. DETAILED DESCRIPTION

[0067] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in "composition" and "application example" are intended to describe specific implementation schemes and do not constitute limitations on the scope of protection of the present invention.

[0068] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the percentages and mass fractions are calculated.

[0069] Some raw materials and their sources are as follows:

[0070] Red maple extract: purchased from LUCAS MEYER COSMETICS;

[0071] Ginger extract: purchased from Croda Chemicals (Shanghai) Co., Ltd.

[0072] Dendrobium officinale extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.;

[0073] North American pine bark extract: purchased from LUCAS MEYER COSMETICS;

[0074] Fleischer's Epilobium extract: purchased from DSM Nutritional Products Ltd.

[0075] The mass proportions of the components in the repair composition are shown in Table 1:

[0076] Table 1 The mass ratio of each component in the repair composition

[0077]

[0078]

[0079] Note: In the above compositions ①-⑩, the missing raw material mass parts are supplemented with an equal amount of deionized water.

[0080] Preparation method of repair composition:

[0081] Weigh the corresponding raw materials according to the mass fraction in Table 1 and mix them evenly.

[0082] Eye cream ingredients and mass percentages are as follows:

[0083] Table 2 Eye cream raw materials and mass percentage

[0084]

[0085] Note: “-” means no addition;

[0086] The surfactant is glyceryl stearate citrate and PEG-100 stearate, and the mass ratio thereof is 1:0.7;

[0087] The moisturizing agent is sodium hyaluronate and butylene glycol, and the mass ratio thereof is 0.1:5;

[0088] The preservatives are hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol, and the mass ratio thereof is 0.4:0.5:1;

[0089] The pH regulator is arginine;

[0090] The thickener is ammonium acryloyldimethyltaurate / VP copolymer;

[0091] The oils and fats are cetearyl alcohol and caprylic / capric triglyceride, and the mass ratio thereof is 1:3.

[0092] Eye cream preparation method:

[0093] S1: Evenly mix the red maple extract, the red ginger extract, the dendrobium candidum extract, the North American pine bark extract, and the Fleischer's epilobium extract to obtain a repairing composition;

[0094] S2: Add the moisturizer and thickener into the emulsifying pot, heat to 80°C, and stir evenly to obtain phase A;

[0095] S3: Add surfactant and oil into an oil pan, heat to 74°C, stir and add into phase A to obtain phase B;

[0096] S4: Add the pH adjuster, preservative and repair composition into phase B, cool to room temperature, stir evenly to obtain eye cream.

[0097] Test 1: Cell migration assay

[0098] The cell migration effects of compositions 1-3 and ①-⑩ were verified.

[0099] The degree of cell migration has an important impact on wound healing and skin repair. In the process of wound healing, cell migration is an important step for epidermal cells to cover the wound, so the higher the cell migration rate, the faster the wound heals. At the same time, cell migration is also involved in the repair process after skin damage. If cell migration is impaired, it will lead to skin repair disorders, which in turn cause various skin diseases.

[0100] Press 1*10 6 Hacat cells were seeded into 12-well plates at a density of 100 units and incubated at 37°C with 5% CO 2 Incubate overnight in an incubator. When the cell plating rate in the 12-well plate reaches about 80%, scratch the sample group and the blank control group. After the scratching, wash the cells with PBS to remove dead cells, add fresh SCF-free keratinocyte culture medium, and add 50 μL of composition 1-3 and ①-⑩ (with deionized sterile water as solvent) to each well at a concentration of 50 mg / L. Use an equal amount of deionized sterile water as a blank control, take pictures and record with an inverted microscope, 37°C, 5% CO 2 The cells were incubated in an incubator for 24 h, and photographed again for statistical analysis using ImageJ software. Three parallel experiments were performed in each sample group and blank control group, and the results were averaged.

[0101] Table 3 Cell migration rate

[0102]

[0103]

[0104] It can be seen from Table 3 that when the technical solution of the present invention is adopted, the obtained composition has a significant cell migration rate, and when the raw material components are missing, the cell migration rate of the obtained composition is significantly reduced.

[0105] Test 2: Tyrosinase inhibition test

[0106] The tyrosinase inhibition test was used to verify the tyrosinase inhibition ability of compositions 1-3, ①-⑩.

[0107] Tyrosinase is a key enzyme in the synthesis of melanin. Inhibiting it can effectively reduce the synthesis of melanin and play a whitening role.

[0108] The test method refers to T / GDCA 006-2021 "Guangdong Cosmetics Association Group Standard-Test Method for Tyrosinase Activity Inhibition of Cosmetic Raw Materials (In Vitro Method)".

[0109] Test samples: Compositions 1-3, compositions ①-⑩, concentration: 50 mg / L (deionized sterile water as solvent), blank is an equal amount of deionized sterile water;

[0110] Kojic acid concentration: 0.005g / L.

[0111] Table 4 Tyrosinase inhibition rate

[0112]

[0113]

[0114] Note: "*" indicates significant difference compared with the blank group, P value < 0.05.

[0115] According to the results of compositions 1-3 in Table 4, the repair compositions provided by the embodiments of the present invention have a good tyrosinase inhibition effect, especially composition 1 has a better effect.

[0116] Comparing the results of composition 1 with compositions ①-⑩, it can be seen that the use of the repair composition with a specific combination and a specific ratio of the present invention can significantly enhance the tyrosinase inhibition effect, and there is a synergistic effect between the raw materials in the repair composition of the present invention.

[0117] Experiment 3: Human efficacy test

[0118] The eye cream prepared by the present invention was subjected to an 8-week anti-aging test according to the method described in the literature (Zhu Liping et al., Clinical efficacy test and analysis of ginseng anti-aging mask [J], Chinese Journal of Aesthetic Medicine, 2016, 25(02): 33-36).

[0119] Ten volunteers were selected for each application case. Inclusion criteria: ① Male or female, aged 30 to 65 years old; with a certain degree of facial / eye skin sagging or fine wrinkles on the face / eyes; ② Good health, serious attitude, certain expression ability, and can truly reflect the feelings after use; ③ Voluntary participation, signing the informed consent form, strictly abide by the requirements of the research plan, use the product according to the requirements of the research plan and complete follow-up. Exclusion criteria: ① Pregnant or lactating women; ② Sensitive skin; ③ People with damaged facial skin or skin diseases that affect the observation of facial status; ④ People who have received skin treatment, beauty and other tests that may affect the results; ⑤ People who have sunburned or used hormone drugs and immunosuppressants in the past 1 month; ⑥ People who have participated in other facial clinical studies or received treatment by dermatologists currently or in the past 3 months. Termination and exclusion criteria: ① The subject requests to stop the test; ② The subject's compliance is poor; ③ The test cannot be continued due to adverse reactions or special physiological and pathological changes; ④ During the test, other skin care products that affect this experiment are used, and excessive exposure to ultraviolet rays are received.

[0120] How to use the product: After cleansing every night, volunteers apply 500-600 mg of the test sample evenly on the face. After massage and absorption, there is no need to cleanse the face or apply any other cosmetics. This process lasts for 8 consecutive weeks: relevant data are measured on the same part at week 0 and week 8.

[0121] Instrument measurement method: The test was conducted using the moisture tester Corneometer, skin moisture loss meter Tewameter, skin elasticity tester MPA580 from German CK Company, and the VISIA facial image analysis system from American Canfield Company.

[0122] ①Measurement of the moisture content of the stratum corneum of the skin (skin moisture content): Use a moisture tester to measure the moisture content of the stratum corneum of the left and right cheeks, and measure 4 times to take the average value;

[0123] ② Measurement of transepidermal water loss (TEWL) of facial skin: Use a skin water loss meter to measure the transepidermal water loss of the left and right faces, and take the average value of the measurement twice;

[0124] ③Facial skin elasticity measurement: Use a skin elasticity tester to measure the skin elasticity at the end of the left and right eyes and under the eyes, and calculate R 2 The average value, R 2 =Ua / Uf; Ua is the recovery value of the skin from the removal of negative pressure to the next continuous test when negative pressure is applied to the skin surface; Uf is the maximum stretching of the skin when negative pressure is present.

[0125] ④ Facial imaging: The VISIA facial image analysis system was used to take photos of the subject's face (front, left and right 45° directions). The wrinkle score and texture score were extracted and the average value was taken.

[0126] Test samples: Application Examples 1-5, Application Example ①.

[0127] Table 5 Human efficacy test results

[0128]

[0129] Test results of the water content of the stratum corneum of the skin: As shown in Table 5, compared with the gradual increase in the water content of the stratum corneum of the skin in week 0, the eye cream prepared by the present invention shows a good moisturizing effect.

[0130] TEWL value (unit: g / hm 2 ) measurement results: It can be seen from Table 5 that compared with the 0th week, the transepidermal water loss of the skin gradually decreased, and the eye cream prepared by the present invention showed a good water-locking effect.

[0131] Test results of skin elasticity: As shown in Table 5, compared with the skin elasticity in week 0, the eye cream prepared by the present invention shows a good effect of improving skin elasticity.

[0132] Test results of skin wrinkle scores: As shown in Table 5, compared with the volunteers in week 0, the facial wrinkle scores of the volunteers were significantly and gradually reduced, indicating that the eye cream prepared by the present invention has a strong effect of improving facial wrinkles and fine lines, and will not produce skin tolerance due to long-term use.

[0133] Therefore, long-term use of the eye cream prepared by the embodiment of the present invention by the subjects can increase the skin moisture content, reduce transepidermal water loss, improve skin elasticity, effectively improve facial wrinkles, and show good anti-aging, wrinkle reduction, and skin elasticity improvement effects.

[0134] Test 4: Safety test:

[0135] The present invention experiments verify the biosafety of Application Examples 1-5.

[0136] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 50 people aged 16-65 years were randomly distributed and tested.

[0137] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0138] Evaluation criteria:

[0139] Grade 0: negative reaction;

[0140] Grade 1: Suspicious reaction, only slight erythema;

[0141] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0142] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0143] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0144] Test results: Skin reactions were negative in all subjects.

[0145] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A repair composition, characterized in that: Contains the following components by mass: Red maple extract: 0.1-6 parts; Ginger extract: 0.1-5 parts; Dendrobium officinale extract: 0.5-25 parts; North American pine bark extract: 0.1-5 parts; Fleischer's Epilobium extract: 0.1-6 parts.

2. The repair composition according to claim 1, characterized in that: Contains the following components by mass: Red maple extract: 1-3 parts; Ginger extract: 0.5-3 parts; Dendrobium officinale extract: 5-15 parts; North American pine bark extract: 0.5-2 parts; Fleischer's Epilobium Extract: 1-3 parts.

3. The repair composition according to claim 1, characterized in that: Contains the following components by mass: Red maple extract: 1-2 parts; Ginger extract: 0.5-1 part; Dendrobium officinale extract: 10-15 parts; North American pine bark extract: 0.5-1 part; Fleischer's Epilobium Extract: 1-2 parts.

4. The repair composition according to claim 2, characterized in that: Contains the following components by mass: Red maple extract: 2 parts; Red Ginger Extract: 1 part; Dendrobium officinale extract: 10 parts; North American pine bark extract: 1 part; Fleischer's Epilobium Extract: 2 parts.

5. Use of the repair composition according to any one of claims 1 to 3 in preparing eye cream.

6. The use according to claim 4, characterized in that: The eye cream comprises the repairing composition according to any one of claims 1 to 3.

7. An eye cream as claimed in claim 4, characterized in that: The eye cream comprises the following components in percentage by weight: 1-10 wt % of the repair composition according to any one of claims 1-3; Surfactant 0.1-5wt%; Moisturizer 1-15wt%; Preservative 0.1-1.5wt%; pH regulator 0.01-0.3wt%; Thickener 0.1-1.5wt%; Grease 1-20wt%; The balance was deionized water.

8. The eye cream according to claim 6, characterized in that: The surfactant is one or more of sucrose polystearate, glyceryl stearate citrate, and PEG-100 stearate; the moisturizer is one or more of sodium hyaluronate, butylene glycol, and glyceryl polyether-26; the preservative is one or more of p-hydroxyacetophenone, 1,2-hexanediol, and 1,2-pentanediol; the pH adjuster is arginine; and the thickener is one or more of carbomer and / or ammonium acryloyldimethyltaurate / VP copolymer.

9. The eye cream according to claim 6, characterized in that: The oil is one or more of cetearyl alcohol, dimethicone, and caprylic / capric triglyceride.

10. A method for preparing an eye cream according to any one of claims 6 to 10, characterized in that: The following steps are involved: S1: Evenly mix the red maple extract, the red ginger extract, the dendrobium candidum extract, the North American pine bark extract, and the Fleischer's epilobium extract to obtain a repairing composition; S2: Add the moisturizer and thickener into the emulsifying pot, heat to 70-90°C, stir evenly, and obtain phase A; S3: Add surfactant and oil into an oil pan, heat to 70-90°C, stir and add into phase A to obtain phase B; S4: Add the pH adjuster, preservative and repair composition into phase B, cool to room temperature, stir evenly to obtain eye cream.

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