Moisturizing and repairing composition

Abstract

The invention discloses a moisturizing and repairing composition, and belongs to the technical field of cosmetics, and the moisturizing and repairing composition provided by the invention comprises the following components in parts by mass: 1-5 parts of capparis spinosa fruit extract; 0.1 to 0.7 part of cryptomeria fortunei bud extract; 0.01 to 5 parts of cocoa grandiflora resin; 1-4 parts of a kelp extract; and 0.01 to 4 parts of zostera marina extract. The moisturizing and repairing composition provided by the invention has the effects of moisturizing, resisting oxidation, promoting cell migration rate and inhibiting bacteria, and the whole moisturizing and repairing composition is mild and non-irritant; the moisturizing and repairing composition provided by the invention can be applied to cosmetics to effectively improve skin, and meanwhile, the composition provided by the invention is natural in component source, simple in preparation method and suitable for actual production.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a moisturizing and repairing composition. Background Art

[0002] As the first line of defense of the human body, the skin plays a role in isolating the external environment and maintaining a constant internal environment. However, with the accelerated pace of modern life and the intensification of environmental pollution, the skin easily loses moisture, which reduces the skin barrier function, leading to dry skin, accelerated aging, and damage to the skin's protective barrier, making it easier for bacteria to enter the surface of the skin and even cause various skin problems. Therefore, moisturizing becomes the first step in protecting the skin barrier, and its importance is self-evident. The main functions of moisturizing include helping the skin restore moisture, enhancing the skin's water retention capacity, thereby maintaining the skin's elasticity, reducing the risk of skin damage, delaying the skin's aging process, and helping to solve a variety of skin problems.

[0003] During the process of skin drying, other skin problems often occur, such as skin oxidation, low cell mobility, insufficient nutrition of skin cells, slow cell metabolism, etc., which lead to failure of the skin barrier. Therefore, in the process of moisturizing, it is necessary to replenish water and adjust the health of the skin at the same time. Therefore, it is necessary to provide a moisturizing and repairing product with multiple functions such as anti-oxidation, antibacterial, and improving skin cell mobility to solve a series of problems that may arise when the skin is dehydrated and the barrier function is reduced. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a moisturizing and repairing composition that can effectively moisturize, nourish the skin, inhibit bacteria and is mild and non-irritating.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a moisturizing and repairing composition, comprising the following components in parts by weight:

[0007] Capparis spinosa fruit extract: 1-5 parts; Cryptomeria japonica bud extract: 0.1-0.7 parts; Theobroma cacao resin: 0.01-5 parts; Giant kelp extract: 1-4 parts; Zostera maria extract: 0.01-4 parts.

[0008] The present invention selects to add a suitable mass ratio of Capparis spinosa fruit extract, Cryptomeria japonica bud extract, Theobroma grandiflora resin, Giant kelp extract, and Zostera marina extract to the moisturizing and repairing composition. These five components can cooperate with each other to solve the problems of dry skin, peeling, shrunken and sensitive skin caused by external environmental factors. Specifically, the components of the moisturizing and repairing composition provided by the present invention cooperate with each other, and after use, it can effectively provide moisturizing, anti-oxidation, promote cell migration rate, and also have antibacterial properties for the skin.

[0009] The present invention has found that the extract of Capparis spinosa fruit is rich in active ingredients such as volatile oils, alkaloids, flavonoids and glucosinolates. Among them, flavonoid compounds such as quercetin have strong antioxidant properties, can promote the production of matrix metalloproteinases such as MMP-1, and help maintain the elasticity of the skin and other tissues; the volatile oil components such as terpenoids can effectively penetrate the skin, help repair and strengthen the skin barrier and reduce water loss, thereby providing a long-lasting moisturizing effect; and ingredients such as glucosinolates help promote the renewal and regeneration of skin cells and improve skin texture.

[0010] Cryptomeria fortunei bud extract is also rich in ketones, terpenes and phenolic compounds, which have a significant effect on improving blood circulation and thus the vitality of skin cells.

[0011] Theobroma cacao resin is rich in oleic acid, stearic acid, palmitic acid, linoleic acid, arachidic acid, etc. In addition, it also contains β-sitosterol, stigmasterol, and campesterol. The above substances can enable theobroma cacao resin to effectively improve skin texture and protect the skin from environmental stimulation. It also has significant anti-wrinkle, anti-inflammatory, skin calming and skin soothing effects.

[0012] Giant kelp extract and Zostera marina extract are rich in active ingredients such as polysaccharides, terpenes, proteins, polyphenols, cyclic sulfur compounds, etc., and have significant biological activities such as antioxidant and moisturizing.

[0013] The above components are used together to achieve excellent moisturizing, skin nourishing and antibacterial effects, and are mild, non-irritating, soothing and calming.

[0014] As a preferred embodiment of the moisturizing and repairing composition of the present invention, the moisturizing and repairing composition comprises the following components in parts by weight:

[0015] Capparis spinosa fruit extract: 2-5 parts; Cryptomeria japonica bud extract: 0.1-0.5 parts; Theobroma cacao resin: 0.01-5 parts; Giant kelp extract: 1-3 parts; Zostera maria extract: 0.01-3 parts.

[0016] The present invention has found that the mass fraction of each component in the moisturizing and repairing composition will affect the performance of the product. When the mass fraction of the components is further selected to be within the above range (including the endpoint value), the obtained product has better moisturizing, skin nourishing and antibacterial effects, as well as mild, non-irritating, soothing and calming characteristics.

[0017] As the most preferred embodiment of the moisturizing and repairing composition of the present invention, the moisturizing and repairing composition comprises the following components by weight:

[0018] Capparis spinosa fruit extract: 2-5 parts; Cryptomeria japonica bud extract: 0.1-0.5 parts; Theobroma cacao resin: 3-5 parts; Giant kelp extract: 1-3 parts; Zostera maria extract: 0.01-3 parts.

[0019] As a preferred embodiment of the moisturizing and repairing composition of the present invention, the preparation method of the Cryptomeria fortunei buds is to use Phellinus igniarius and Saccharomyces paradoxus to ferment successively, and the specific steps are as follows:

[0020] A1: Wash and dry the Cryptomeria fortunei buds, crush them using a micro air flow pulverizer, and then pass through a 60-400 mesh sieve to obtain X1;

[0021] A2: Add 5-10 times the mass of deionized sterile water to X1 and homogenize to obtain culture solution X2;

[0022] A3: adding 0.1-2 wt% of hop spore ball powder in an amount of X1 to X2, and homogenizing to obtain culture solution X3;

[0023] A4: inoculate 1-4 wt% of the mass of X1 into X3, and ferment for 12-18 h to obtain fermentation liquid X4;

[0024] A5: Use an ultrasonic cell wall breaker to break the cell wall of X4, and then obtain culture medium X5;

[0025] A6: Inoculate X5 with 3-5 wt% of the mass of X1 of Saccharomyces cerevisiae, ferment for 6-8 h, and then break the cell wall with an ultrasonic cell breaker to obtain culture solution X6;

[0026] A7: X6 was sterilized at ultra-high temperature, and then filtered. The filtrate was freeze-dried to obtain Cryptomeria fortunei bud extract X7.

[0027] The present invention has found that, during the fermentation of Cryptomeria fortunei buds, adding hop spore leaf ball powder can effectively increase the enrichment of phenolic and flavonoid products in the fermentation product; using Phellinus igniarius and Saccharomyces cerevisiae for fermentation can make the active ingredients of natural products in the fermentation broth more complex and diverse. When commercially available and homemade Cryptomeria fortunei bud extracts of the present invention are respectively added to the moisturizing and repairing composition, there will be differences in the efficacy of the obtained moisturizing and repairing composition. When the homemade Cryptomeria fortunei bud extract of the present invention is selected, the comprehensive effect of the moisturizing and repairing composition is better.

[0028] In a second aspect of the present invention, the present invention provides a cosmetic comprising the moisturizing and repairing composition of the present invention.

[0029] As a preferred embodiment of the cosmetics of the present invention, the cosmetics include the following components in mass percentage: moisturizing and repairing composition: 1wt%-5wt%; antioxidant: 0.2wt%-0.5wt%; surfactant: 0.1wt%-5wt%; thickener: 0.1wt%-0.5wt%; emollient: 0.1wt%-5wt%; preservative: 0.1wt%-3wt%, and the balance is deionized water.

[0030] Preferably, the antioxidant is one or more of vitamin E, coenzyme Q10, and carotenoids.

[0031] Preferably, the surfactant is one or more of glyceryl stearate, PEG-100 stearate, cetearyl olivate, sorbitan olivate, sodium stearoyl glutamate, palmitic acid, stearic acid, Tween 80, and PEG-40 hydrogenated castor oil.

[0032] Preferably, the thickener is one or more of carrageenan, guar gum, carbomer and xanthan gum.

[0033] Preferably, the emollient is one or more of caprylic / capric triglyceride, isononyl isononanoate, and coconut oil alcohol-caprylate / caprate.

[0034] Preferably, the preservative is one or more of phenoxyethanol, p-hydroxyacetophenone, and 1,2-hexanediol.

[0035] As a preferred embodiment of the cosmetics of the present invention, the cosmetics include any one of lotion, emulsion, cream, mask, essence, and spray.

[0036] In a third aspect of the present invention, the present invention provides a method for preparing a cosmetic, the preparation method comprising the following steps:

[0037] 1. Add thickener, surfactant and deionized water into the emulsifier, heat to 60±10℃, stir evenly, heat emollient and antioxidant to 60±10℃ at the same time, pour into the emulsifier, and homogenize until completely mixed;

[0038] 2. Cool component A to room temperature, add the moisturizing and repairing composition and the preservative, stir evenly, then stop stirring, discharge the material, and obtain the cosmetics.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The present invention provides a moisturizing and repairing composition, wherein the five components in the composition can cooperate with each other and can achieve full-stage moisturizing, skin nourishing and antibacterial effects within a given range. Specifically, firstly, the moisturizing and repairing composition can effectively nourish the skin, repair the skin barrier, and reduce the transepidermal water loss rate; secondly, the moisturizing and repairing composition can effectively resist oxidation, inhibit bacteria, maintain the skin barrier, and reduce skin sensitivity; thirdly, the moisturizing and repairing composition is mild and non-irritating as a whole, has a wide range of applicable objects, and has a simple preparation method, which is suitable for actual production. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 : Contents of total flavonoids and total phenols in the fermentation products of Phellinus igniarius with and without hop spore powder.

[0042] Note: Figure 1 The material ratio and experimental steps of the fermentation experiment are consistent with those of Example 1. The raw material for determination is fermentation broth X4, the total flavonoids determination method is aluminum nitrate complex spectrophotometry for determining total flavonoids, and the total phenol determination method is Folin phenol method. DETAILED DESCRIPTION

[0043] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0044] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0045] Some raw materials and their sources are as follows:

[0046] Capparis spinosa fruit extract: purchased from Shanghai Jiakai Biotechnology Co., Ltd.;

[0047] Cryptomeria fortunei bud extract: Shanghai Puen Biochemical Technology Co., Ltd.;

[0048] Large flower cocoa resin: Guangzhou Hean Chemical Co., Ltd.;

[0049] Cryptomeria fortunei buds: purchased from Guangzhou Huayu Biotechnology Co., Ltd.;

[0050] Giant kelp extract: purchased from Guangzhou Huayu Biotechnology Co., Ltd.;

[0051] Zostera marina extract: purchased from Guangzhou Huayu Biotechnology Co., Ltd.;

[0052] Phellinus igniarius strain: purchased from Ningbo Testo Biotechnology Co., Ltd., catalog number TS327473;

[0053] Saccharomyces cerevisiae strain: purchased from Ningbo Testo Biotechnology Co., Ltd., catalog number TS301993;

[0054] Hops conospore ball powder: purchased from Shaanxi Yuanbeibei Biotechnology Co., Ltd.

[0055] Preparation of Cryptomeria japonica bud extract:

[0056] Embodiment 1:

[0057] A1: Wash and dry the Cryptomeria fortunei buds, crush them using a micro air flow grinder, and pass through a 350-mesh sieve to obtain X1;

[0058] A2: Add 7.5 times the mass of deionized sterile water to X1 and homogenize to obtain culture solution X2;

[0059] A3: Add 1 wt% of hop spore ball powder to X2, and homogenize to obtain culture solution X3;

[0060] A4: Inoculate X3 with 2.7 wt% of the mass of X1 and ferment for 14 h to obtain fermentation liquid X4;

[0061] A5: Use an ultrasonic cell wall breaker to break the cell wall of X4, and then obtain culture medium X5;

[0062] A6: Inoculate X5 with 4 wt% of the mass of X1 with Saccharomyces cerevisiae, ferment for 7 h, and then break the cell wall with an ultrasonic cell breaker to obtain culture solution X6;

[0063] A7: X6 was sterilized at ultra-high temperature, and then filtered. The filtrate was freeze-dried to obtain Cryptomeria fortunei bud extract X7.

[0064] Embodiment 2:

[0065] A1: Wash and dry the Cryptomeria fortunei buds, crush them using a micro air flow pulverizer, and then pass through a 60-mesh sieve to obtain X1;

[0066] A2: Add 5 times the mass of deionized sterile water to X1 and homogenize to obtain culture solution X2;

[0067] A3: Add 0.1 wt% of hop spore ball powder in an amount of X1 to X2, and homogenize to obtain culture solution X3;

[0068] A4: Inoculate 1 wt% of the mass of X1 into X3 with Phellinus igniarius, and ferment for 12 h to obtain fermentation liquid X4;

[0069] A5: Use an ultrasonic cell wall breaker to break the cell wall of X4, and then obtain culture medium X5;

[0070] A6: Inoculate X5 with 5 wt% of the mass of X1 of Saccharomyces cerevisiae, ferment for 8 h, and then break the cell wall with an ultrasonic cell breaker to obtain culture solution X6;

[0071] A7: X6 was sterilized at ultra-high temperature, and then filtered. The filtrate was freeze-dried to obtain Cryptomeria fortunei bud extract X7.

[0072] Embodiment 3:

[0073] A1: Wash and dry Cryptomeria fortunei buds, crush the dried Cryptomeria fortunei buds using a micro air flow pulverizer, and then pass through a 400-mesh sieve to obtain X1;

[0074] A2: Add deionized sterile water 10 times the mass of X1 to X1 and homogenize to obtain culture solution X2;

[0075] A3: Add 2 wt% of hop spore ball powder to X2, and homogenize to obtain culture solution X3;

[0076] A4: Inoculate X3 with 4 wt% of the mass of X1 and ferment for 18 h to obtain fermentation liquid X4;

[0077] A5: Use an ultrasonic cell wall breaker to break the cell wall of X4, and then obtain culture medium X5;

[0078] A6: Inoculate X5 with 3 wt% of the mass of X1 of Saccharomyces cerevisiae, ferment for 6 h, and then break the cell wall with an ultrasonic cell breaker to obtain culture solution X6;

[0079] A7: X6 was sterilized at ultra-high temperature, and then filtered. The filtrate was freeze-dried to obtain Cryptomeria fortunei bud extract X7.

[0080] Comparative Example 1: The difference from Example 1 is that no hop spore leaf ball powder is added;

[0081] Comparative Example 2: The difference from Example 1 is that only Phellinus igniaris is used for fermentation;

[0082] Comparative Example 3: The difference from Example 1 is that only Saccharomyces cerevisiae was used for fermentation;

[0083] Comparative Example 4: The difference from Example 1 is that the fermentation with Saccharomyces cerevisiae is carried out first, and then the fermentation with Phellinus igniarius is carried out.

[0084] The mass proportions of the components in the moisturizing and repairing composition are as follows: Examples 4-8 and Comparative Examples 5-17:

[0085] Embodiment 4:

[0086] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Example 1); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0087] Embodiment 5:

[0088] Capparis spinosa fruit extract: 5 parts; Cryptomeria japonica bud extract: 0.1 parts (Example 1); Theobroma cacao resin: 0.01 parts; Giant kelp extract: 1 part; Zostera maria extract: 4 parts.

[0089] Embodiment 6:

[0090] Capparis spinosa fruit extract: 1 part; Cryptomeria japonica bud extract: 0.7 part (Example 1); Theobroma cacao resin: 5 parts; Giant kelp extract: 4 parts; Zostera maria extract: 0.01 part.

[0091] Example 7

[0092] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Example 2); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0093] Example 8

[0094] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Example 3); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0095] Comparative Example 5:

[0096] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Comparative Example 1); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0097] Comparative Example 6:

[0098] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Comparative Example 2); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0099] Comparative Example 7:

[0100] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Comparative Example 3); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0101] Comparative Example 8:

[0102] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Comparative Example 4); Theobroma cacao resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0103] Comparative Example 9:

[0104] Capparis spinosa fruit extract: 2 parts; Theobroma cacao butter: 0.5 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0105] Comparative Example 10:

[0106] Capparis spinosa fruit extract: 2 parts; Cryptomeria fortunei bud extract: 0.5 parts (Example 1); Theobroma cacao resin: 3 parts.

[0107] Comparative Example 11:

[0108] Cryptomeria fortunei bud extract: 0.5 parts (Example 1); Theobroma grandiflora resin: 3 parts; Giant kelp extract: 2 parts; Zostera maria extract: 2 parts.

[0109] Comparative Example 12:

[0110] Capparis spinosa fruit extract: 2 parts; Cryptomeria japonica bud extract: 0.5 parts (Example 1); Giant kelp extract: 2 parts; Zostera marina extract: 2 parts.

[0111] Comparative Example 13: Cryptomeria fortunei bud extract: 0.5 parts (Example 1).

[0112] Comparative Example 14: Zostera marina extract: 2 parts; Giant kelp extract: 2 parts.

[0113] Comparative Example 15: Capparis spinosa fruit extract: 2 parts.

[0114] Comparative Example 16: 3 parts of large-flowered theobroma cacao resin.

[0115] Comparative Example 17: Capparis spinosa fruit extract: 1 part; Cryptomeria japonica bud extract: 0.7 parts (commercially available); Theobroma cacao resin: 5 parts; Giant kelp extract: 4 parts; Zostera maria extract: 1 part.

[0116] In the above comparative examples 9-16, the missing parts by mass of raw materials were made up with deionized sterile water.

[0117] The cosmetic raw materials and mass percentages are as follows: Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples:

[0118] Application Example 1: 3 wt% of moisturizing and repairing composition (Example 4); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0119] Application Example 2: 3 wt% of moisturizing and repairing composition (Example 5); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0120] Application Example 3: 3 wt% of moisturizing and repairing composition (Example 6); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0121] Application Example 4: 3 wt% of moisturizing and repairing composition (Example 7); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0122] Application Example 5: 3 wt% of moisturizing and repairing composition (Example 8); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0123] Application Example 6: 1 wt% of the moisturizing and repairing composition (Example 4); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0124] Application Example 7: 5 wt% of the moisturizing and repairing composition (Example 4); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0125] Comparative Application Example 1: 3 wt% of moisturizing and repairing composition (Comparative Example 5); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0126] Comparative Application Example 2: 3 wt% of moisturizing and repairing composition (Comparative Example 6); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0127] Comparative Application Example 3: 3 wt% of moisturizing and repairing composition (Comparative Example 7); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0128] Comparative Application Example 4: 3 wt% of moisturizing and repairing composition (Comparative Example 8); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0129] Comparative Application Example 5: 3 wt% of moisturizing and repairing composition (Comparative Example 9); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0130] Comparative Application Example 6: 3 wt% of moisturizing and repairing composition (Comparative Example 10); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0131] Comparative Application Example 7: 3 wt% of moisturizing and repairing composition (Comparative Example 11); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0132] Comparative Application Example 8: 3 wt% of moisturizing and repairing composition (Comparative Example 12); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0133] Comparative Application Example 9: 3 wt% of moisturizing and repairing composition (Comparative Example 13); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0134] Comparative Application Example 10: 3 wt% of moisturizing and repairing composition (Comparative Example 14); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0135] Comparative Application Example 11: 3 wt% of moisturizing and repairing composition (Comparative Example 15); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0136] Comparative Application Example 12: 3 wt% of moisturizing and repairing composition (Comparative Example 16); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0137] Comparative Application Example 13: 3 wt% of moisturizing and repairing composition (Comparative Example 17); 0.3 wt% of antioxidant; 0.5 wt% of surfactant; 0.3 wt% of thickener; 2 wt% of emollient; 1 wt% of preservative, and the balance is made up with deionized water.

[0138] Blank application example: antioxidant 0.3wt%; surfactant 0.5wt%; thickener 0.3wt%; emollient 2wt%; preservative 1wt%, the balance is made up with deionized water.

[0139] Among them, in the application examples, comparative application examples, and blank application examples, the surfactants are specifically glyceryl stearate and sorbitan olivate, and their specific mass fraction ratio is 1:0.4; the antioxidants are specifically vitamin E and coenzyme Q10, and their specific mass fraction ratio is 1:0.7; the thickener is specifically carrageenan; the emollients are specifically caprylic / capric triglyceride, isononyl isononanoate, and coconut oil caprylate / caprate, and their mass fraction ratio is 1:0.5:0.3, and the water is deionized sterile water; the preservative is phenoxyethanol.

[0140] The preparation method of Application Example 1 is specifically as follows:

[0141] 1. Add thickener, surfactant and deionized water into the emulsifier, heat to 60±10℃, stir evenly, heat emollient and antioxidant to 60±10℃ at the same time, pour into the emulsifier, and homogenize until completely mixed;

[0142] 2. Cool component A to room temperature, add the moisturizing and repairing composition and the preservative, stir evenly, then stop stirring, discharge the material, and obtain the cosmetics.

[0143] The preparation methods of Application Examples 2-7, Comparative Application Examples 1-13, and Blank Application Examples are consistent with that of Application Example 1.

[0144] Test 1

[0145] Experiment 1 of the present invention verifies the inhibitory effects of Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples on hyaluronidase.

[0146] Hyaluronic acid, also known as hyaluronic acid, is a powerful moisturizer. It can attract and lock in moisture, maintain skin moisture, and improve dry, rough and dehydrated skin conditions. Hyaluronic acid can also enhance the biological activity of cells and has a strong synergistic effect on repairing and protecting the skin. Therefore, by inhibiting hyaluronidase, the decomposition rate of hyaluronic acid in the skin is reduced, thereby achieving long-term moisture retention and repair of the skin, which is of great significance.

[0147] (1) Take four groups of clean test tubes, label them A, B, C, and D, add 50 μL of the sample to be tested at 10 mg / L to test tubes A and B, add 50 μL of distilled water to test tubes C and D, add 50 μL of 500 U / mL hyaluronidase solution to test tubes A and C, add 50 μL of pH 5.6 acetate buffer to test tubes B and D, and place the test tubes in a 37°C incubator for 20 min;

[0148] (2) Add 10 μL of 2.5 mol / L CaCl2 solution to each of the four test tubes and place them in a 37°C incubator for 20 min;

[0149] (3) Add 50 μL of 0.5 mg / mL sodium hyaluronate solution to test tubes A and C, and add 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37°C incubator for 40 min, then take them out and place them at room temperature for 10 min.

[0150] (4) Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, and 50 μL of acetylacetone solution to the four test tubes respectively, place the test tubes in a boiling water bath for 30 min, then place them in an ice bath for 10 min, and finally place them at room temperature for 10 min;

[0151] (5) Add 100 μL of P-DAB reagent to each test tube, and then measure the absorbance at 530 nm using an ELISA reader.

[0152]

[0153] Wherein: A is the OD value of the sample solution; B is the OD value of the sample blank; C is the OD value of the control sample solution; D is the OD value of the control sample blank.

[0154] Table 1 Moisturizing effect test

[0155]

[0156] It can be seen from Table 1 that when the technical solution of the present invention is adopted, the inhibition rate of the obtained composition on hyaluronidase is relatively high, at more than 72.53%. When the raw material components are missing or the extraction process not specified by the present invention is used to extract the Cryptomeria fortunei bud extract, the inhibition rate of the obtained composition on hyaluronidase is significantly reduced.

[0157] Verify the antioxidant effects of Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples.

[0158] Take 5mL of 7mmol / LABTS+ and 88μL of 140mmo l / L potassium persulfate and mix them evenly to prepare ABTS+ free radical stock solution, and store it in the dark for later use. Take 0.1mL of 10mg / L Application Example 1-7, Comparative Application Example 1-12, and Blank Application Example, add 3.9mL ABTS+ working solution, shake for 30s, let stand at room temperature for 6min, use anhydrous ethanol as a control, measure the absorbance at 734nm, and use Vc as a positive control. Repeat the measurement for each sample 3 times, and calculate the scavenging rate of ABTS+ free radicals according to the following formula:

[0159]

[0160] Where Ai is the absorbance value of the test solution; Aj is the background value of the test solution; A0 is the control value without adding the test solution.

[0161] Table 2 Antioxidant effect

[0162]

[0163] Note: * indicates that the difference is statistically significant compared with the blank group (p < 0.05)

[0164] As can be seen from Table 2, when the technical scheme of the present invention is adopted, the obtained composition has significant antioxidant properties, and its effect is significantly compared with the blank group. When the raw material components are missing or the extraction process not specified by the present invention is used to extract the Cryptomeria fortunei bud extract, the antioxidant activity of the obtained composition is significantly reduced.

[0165] The results of Application Example 1 and Comparative Application Example 1 show that hop spore leaf ball powder can effectively improve the antioxidant property of the composition, which indirectly confirms that it can enrich the polyphenols or flavonoids products in the fermentation process of Phellinus igniarius.

[0166] The results of Application Example 1 and Comparative Application Example 13 show that there are significant differences between the commercially available Cryptomeria fortunei bud extract and the Cryptomeria fortunei bud extract prepared by the present invention.

[0167] The results of Application Example 1 and Comparative Application Examples 2-3 show that the antioxidant properties of the composition prepared by the product obtained by double-strain secondary fermentation are significantly better than those of single-strain fermentation.

[0168] The results of Application Example 1 and Comparative Application Example 4 show that the fermentation sequence of the strains will affect the antioxidant properties of the prepared compounds.

[0169] The results of Application Example 1 and Comparative Application Examples 5-13 show that the components of the composition provided by the present invention have a significant synergistic effect, and the lack of any one of them will lead to a significant decrease in its antioxidant activity.

[0170] The results of Application Example 1 and the blank application example show that the composition provided by the present invention has significant antioxidant effect.

[0171] Test 3

[0172] Verify the antibacterial effects of Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples.

[0173] Using the inhibition ring test, a 24-hour fresh slant culture of Candida albicans was washed with PBS and diluted to 5.0×10 5 CFU / mL-5.0×10 6 CFU / mL is reserved.

[0174] Preparation of antibacterial sheets: Cut filter paper sheets with a diameter of 5 mm and a thickness not exceeding 4 mm, and immerse each group of 4 sheets in 10 mL of sample solution.

[0175] Preparation of negative control samples: Take a sample of the same material without antibacterial ingredients, make a disc (block) of the same size as the test group, and soak it in sterile water.

[0176] Use a sterile cotton swab to apply 5.0×10 5 CFU / mL-5.0×10 6CFU / mL Candida albicans suspension is evenly applied 3 times on the surface of a suitable culture medium plate. Each time it is applied, the plate should be rotated 60°, and finally the cotton swab should be applied around the edge of the plate. Cover the plate and dry it at room temperature for 5 minutes. For each test, place 1 infected plate, 4 test samples and 1 negative control sample on each plate, for a total of 5 samples. Use sterile tweezers to take the sample and paste it on the surface of the plate. The negative control sample is pasted in the center of the plate, and the test sample is pasted around. After pasting, use sterile tweezers to gently press the sample to make it close to the surface of the plate. The distance between the centers of each sample is more than 25mm, and the distance from the periphery of the plate is more than 15mm. Cover the plate, incubate at 36℃±1℃ for 16h~18h to observe the results, and repeat the test 3 times. Use a vernier caliper to measure the diameter of the antibacterial ring (including the patch) and record it. When measuring the antibacterial ring, a uniform and completely sterile antibacterial ring should be selected, and the diameter should be measured based on the outer edge of the antibacterial ring.

[0177] The negative control sample should not produce an antibacterial ring. If the diameter of the antibacterial ring of the test sample is greater than 7mm, it is judged to have an antibacterial effect; if the diameter of the antibacterial ring is ≤7mm, it is judged to have no antibacterial effect. If the antibacterial effect is found in 3 repeated tests, it is judged to be qualified.

[0178] Table 3 Antibacterial test results

[0179]

[0180] “-” means no obvious antibacterial effect; “+” means there is an antibacterial effect, and the antibacterial effect increases with the “+”.

[0181] It can be seen from Table 3 that when the technical scheme of the present invention is adopted, the obtained composition has significant antibacterial performance, and its effect is significantly compared with the blank group. When the raw material components are missing or the extraction process not specified by the present invention is used to extract the Cryptomeria fortunei bud extract, the antibacterial activity of the obtained composition is significantly reduced.

[0182] Test 4

[0183] Verify the cell migration effects of Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples.

[0184] The degree of cell migration has an important impact on wound healing and skin repair. In the process of wound healing, cell migration is an important step for epidermal cells to cover the wound, so the higher the cell migration rate, the faster the wound heals. At the same time, cell migration is also involved in the repair process after skin damage. If cell migration is impaired, it will lead to skin repair disorders, which in turn cause various skin diseases.

[0185] Hacat cells were inoculated into 12-well plates at the corresponding inoculation density and incubated overnight in a 37°C, 5% CO2 incubator. When the cell plating rate in the 12-well plate reached about 80%, the sample group and the solvent control group were scratched. After the scratching, the cells were washed with PBS to remove dead cells, and then fresh EGF-free keratinocyte culture medium was added. According to application examples 1-7, comparative application examples 1-13, and blank application examples, 10 mg / L 50 μL was added, and photos were taken using an inverted microscope. Incubate in a 37°C, 5% CO2 incubator for 24 hours, take photos again, and use ImageJ software for statistical analysis.

[0186] Table 4 Cell migration rate

[0187]

[0188] It can be seen from Table 4 that when the technical solution of the present invention is adopted, the obtained composition has a significant cell migration rate. When the raw material components are missing or the extraction process not specified by the present invention is used to extract the Cryptomeria fortunei bud extract, the cell migration rate of the comparative application example prepared by the obtained composition is significantly decreased.

[0189] Test 5

[0190] The present invention experiments verify the immediate human body repair effects of Application Examples 1-7, Comparative Application Examples 1-13, and Blank Application Examples.

[0191] Transepidermal water loss (TEWL) is an important parameter for evaluating skin barrier function. It indicates the loss of water from the deep dermis through evaporation of the epidermis. The higher the TEWL, the more water is lost through the skin and the weaker the skin's moisturizing ability.

[0192] The transepidermal water loss rate is closely related to the hydration of the stratum corneum and is a commonly used indicator to reflect the barrier function of the stratum corneum. In the development of cosmetics, the efficacy of moisturizing cosmetics can be evaluated by testing the TEWL value of skin water loss.

[0193] 200 healthy subjects aged 18-60 were selected and divided into groups, each group had 10 people, and each group had 5 males and 5 females. In the experiment, half face test was carried out according to the random table, and the left and right nasolabial grooves of the volunteers were selected for skin moisture content (%) and water loss (TEWL) (%) test, and the average of the three values ​​was taken as the data of left and right nasolabial groove skin water loss (TEWL) (T1). After the test, the volunteers were required to use it as required for 28 consecutive days, and the subjects were required to return the test on the 28th day of the experiment (T2).

[0194]

[0195] Table 5 Relative transepidermal water loss

[0196]

[0197] Human experiments have verified that the human efficacy effects of the application examples prepared in the present application are mutually corroborated with the data of experiments 1-4, proving that the moisturizing and repairing composition prepared in the present application does have moisturizing, anti-oxidation, antibacterial, and cell migration rate enhancing effects, and that there is a synergistic effect between the ingredients in the moisturizing and repairing composition of the present application.

[0198] Safety test:

[0199] The present invention experiments verify the biosafety of Application Examples 1-7.

[0200] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 70 people aged 16-65 years were randomly distributed and tested.

[0201] Test method: Place the test substance in a spot tester at a dosage of 0.020-0.025g.

[0202] The patch tester is covered with non-irritating cloth-based tape on the back or curved side of the forearm of the subject, and gently pressed with the palm of the hand to make it evenly adhere to the skin surface for 24 hours. After 30 minutes after the patch tester is removed, observe the skin reaction after the indentation disappears. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0203] Evaluation criteria:

[0204] Grade 0: negative reaction;

[0205] Grade 1: Suspicious reaction, only slight erythema;

[0206] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0207] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0208] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0209] Test results: Skin reactions were negative in all subjects.

[0210] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A moisturizing and repairing composition, characterized in that: The composition comprises the following components by weight: Capparis spinosa fruit extract: 1-5 parts; Cryptomeria fortunei bud extract: 0.1-0.7 parts; Theobroma cacao resin: 0.01-5 parts; Giant kelp extract: 1-4 parts; Zostera marina extract: 0.01-4 parts; The extraction process of the Cryptomeria fortunei bud extract is to use a mixed fermentation of Saccharomyces cerevisiae and Phellinus linteus, and the specific fermentation steps are as follows: A1: Wash and dry the Cryptomeria fortunei buds, crush them using a micro air flow pulverizer, and then pass through a 60-400 mesh sieve to obtain X1; A2: Add 5-10 times the mass of deionized sterile water to X1 and homogenize to obtain culture solution X2; A3: adding 0.1-2 wt% of hop spore ball powder in an amount of X1 to X2, and homogenizing to obtain culture solution X3; A4: inoculate 1-4 wt% of the mass of X1 into X3, and ferment for 12-18 h to obtain fermentation liquid X4; A5: Use an ultrasonic cell wall breaker to break the cell wall of X4, and then obtain culture medium X5; A6: Inoculate X5 with 3-5 wt% of the mass of X1 of Saccharomyces cerevisiae, ferment for 6-8 h, and then break the cell wall with an ultrasonic cell breaker to obtain culture solution X6; A7: X6 was sterilized at ultra-high temperature, and then filtered. The filtrate was freeze-dried to obtain Cryptomeria fortunei bud extract X7.

2. The moisturizing and repairing composition according to claim 1, characterized in that: The composition comprises the following components by weight: Caper fruit extract: 2-5 parts; Cryptomeria fortunei bud extract: 0.1-0.5 parts; Theobroma cacao resin: 0.01-5 parts; Giant kelp extract: 1-3 parts; Zostera marina extract: 0.01-4 parts.

3. The moisturizing and repairing composition according to claim 2, characterized in that: The composition comprises the following components by weight: Caper fruit extract: 2-5 parts; Cryptomeria fortunei bud extract: 0.1-0.5 parts; Theobroma cacao resin: 3-5 parts; Giant kelp extract: 1-3 parts; Zostera marina extract: 0.01-4 parts.

4. A cosmetic, characterized in that: The invention comprises the moisturizing and repairing composition according to any one of claims 1 to 3.

5. The cosmetic according to claim 4, characterized in that: Including the following raw materials in mass percentage: Moisturizing and repairing composition: 1wt%-5wt%; Antioxidant: 0.2wt%-0.5wt%; Surfactant: 0.1wt%-5wt%; Thickener: 0.1wt%-0.5wt%; Emollient: 0.1wt%-5wt%; Preservative: 0.1wt%-3wt%; The balance was deionized water.

6. The cosmetic according to claim 5, characterized in that: The antioxidant is one or more of vitamin E, coenzyme Q10 and carotenoids.

7. The cosmetic according to claim 5, characterized in that: The surfactant is one or more of glyceryl stearate, PEG-100 stearate, cetearyl olive oil, sorbitan olive oil, sodium stearoyl glutamate, palmitic acid, stearic acid, Tween 80, and PEG-40 hydrogenated castor oil.

8. The cosmetic according to claim 5, characterized in that: The thickener is one or more of carrageenan, guar gum, carbomer and xanthan gum.

9. The cosmetic according to claim 5, characterized in that: The preservative is one or more of phenoxyethanol, p-hydroxyacetophenone, and 1,2-hexanediol.

10. The cosmetic according to claim 5, characterized in that: The emollient is one or more of caprylic / capric triglyceride, isononyl isononanoate, and coconut oil alcohol-caprylate / caprate.

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