Composition for preventing and treating acne and application thereof

Abstract

The invention discloses a composition for preventing and treating acnes and application thereof, and the composition for preventing and treating acnes comprises the following components in parts by mass: 6-12 parts of a peach leaf-eucommia ulmoides compound extract, 1-3 parts of vitamin E, 8-15 parts of aloe vera gel and 70-85 parts of hydrogen-rich water. The peach leaf-eucommia ulmoides compound extract, the vitamin E, the aloe vera gel and the hydrogen-rich water are compounded, all the components supplement one another, the functions of resisting oxidation, resisting inflammation, preserving moisture and the like are comprehensively exerted, acne can be effectively prevented and treated, skin healing and repairing can be promoted, and the skin care effect is good. Different components form a multi-layer synergistic effect by inhibiting bacteria, reducing inflammation, protecting skin cells and enhancing a skin barrier function.

Description

Technical Field

[0001] The present invention relates to the technical field of acne prevention and treatment, and in particular to a composition for preventing and treating acne and application thereof. Background Art

[0002] Acne is a chronic inflammatory skin disease that is prone to occur in the pilosebaceous glands of the cheeks, chest, and back. It is characterized by repeated attacks, long course of disease, and strong antibiotic dependence. Its clinical symptoms include whiteheads, papules, pustules, cysts, and nodules, and it is prone to sunken or hypertrophic scars. Modern medicine believes that the onset of acne is mainly related to vigorous secretion of sebaceous glands, increased keratinization of pilosebaceous gland ducts, proliferation of Propionibacterium acnes, and abnormal expression of hormones and their receptors. The hormones that cause acne include sex hormones, insulin, insulin-like growth factor, and thyroid hormone. Propionibacterium acnes is a Gram-positive facultative anaerobic bacterium and a common bacterium in the skin microbial population. It is an opportunistic pathogen that usually does not threaten the skin, but when the skin microecology changes, Propionibacterium acnes can multiply in large numbers and produce a biofilm composed of extracellular polysaccharides, which increases the adhesion of Propionibacterium acnes to the follicular wall and induces the formation of Propionibacterium acnes colonies, increasing the difficulty of acne prevention and treatment.

[0003] In the prior art, oral or topical antibiotics or anti-inflammatory drugs are used to kill acne-related bacteria, inhibit the occurrence of inflammatory reactions, and then inhibit the further development of acne, which plays a role in preventing and treating acne. The most commonly used drugs are tetracycline, clindamycin, benzoyl peroxide, sulfur, omeganam hydrochloride and azelaic acid. In actual use, it is found that the use of antibiotics may break the balance of normal microorganisms on the skin, thereby causing side effects such as rashes and allergies; and although drugs such as sulfur, benzoyl peroxide and azelaic acid have been used for many years, they have been found to be not very effective in actual use, and due to their high irritation, they are prone to side effects such as dry skin and exfoliative dermatitis. Therefore, the development of new anti-acne drugs with good efficacy and high safety has become a hot topic in recent years.

[0004] In recent years, the potential of plant-derived active substances in the prevention and treatment of acne has gradually attracted the attention of the scientific community and consumers. Due to their diverse chemical composition and biological activity, plant extracts can effectively inhibit pathogenic microorganisms and regulate skin inflammatory responses. For example, wild bitter melon extract and oregano extract have been shown to have an inhibitory effect on mouse ear swelling caused by Propionibacterium acnes infection in vivo; Kaempferia parviflora extract has a significant effect on relieving acne inflammation by inhibiting Propionibacterium acnes and its induced inflammatory response. However, the efficacy of a single plant extract is often limited, and a combination of multiple plant extracts that has not been scientifically verified may weaken its effect due to the complex interactions between the components. Therefore, it is particularly important to find a combination of plant extracts with obvious synergistic effects. Summary of the invention

[0005] In view of the above prior art, the object of the present invention is to provide a composition for preventing and treating acne and its application.

[0006] To achieve the above object, the present invention adopts the following technical solution:

[0007] The first aspect of the present invention provides a composition for preventing and treating acne, comprising the following components in parts by weight: 6-12 parts of peach leaf-eucommia ulmoides composite extract, 1-3 parts of vitamin E, 8-15 parts of aloe vera gel, and 70-85 parts of hydrogen-rich water.

[0008] Preferably, the peach leaf-eucommia ulmoides composite extract is prepared by the following method:

[0009] (1) Peach leaves and Eucommia bark are crushed and mixed in a mass ratio of (3-7): (3-5) to obtain a mixture, distilled water and a complex enzyme consisting of cellulase and pectinase are added to the mixture, and after mixing, enzymolysis reaction is performed to obtain an enzymolysis solution;

[0010] (2) adding a mixed seed liquid consisting of a Lactobacillus rhamnosus seed liquid, a Bifidobacterium longum seed liquid, and a Saccharomyces cerevisiae seed liquid to the enzymatic hydrolyzate for fermentation to obtain a fermentation liquid;

[0011] (3) Ultrasonic extraction of the fermentation liquid for 10-45 min, filtering the extract to obtain the supernatant, concentrating and drying to obtain a peach leaf-eucommia composite extract.

[0012] Preferably, in step (1), the amount of distilled water added is 10-15 times the mass of the mixture; the amount of complex enzyme added is 0.5-1% of the mass of the mixture, and the complex enzyme is a mixture of cellulase and pectinase in a mass ratio of (2-4): (1-3).

[0013] Preferably, in step (1), the temperature of the enzymatic hydrolysis reaction is 40-60°C and the time is 2-4h.

[0014] Preferably, in step (2), the amount of mixed seed liquid added is 5%-10% of the volume of the enzymatic solution; in the mixed seed liquid, the volume ratio of Lactobacillus rhamnosus seed liquid, Bifidobacterium longum seed liquid and Saccharomyces cerevisiae seed liquid is (3-5): (4-6): (2-4).

[0015] Preferably, the viable count of Lactobacillus rhamnosus seed solution is ≥ 1.2×10 8 cfu / ml; the number of viable bacteria in the seed solution of Bifidobacterium longum ≥ 2×10 8 cfu / ml; the number of viable bacteria in the brewer's yeast seed solution is ≥2.4×10 8 cfu / ml.

[0016] Preferably, in step (2), the fermentation temperature is 30-37° C. and the fermentation time is 36-60 h.

[0017] Preferably, in step (3), the extraction time of ultrasonic extraction is 15-30 min, and the ultrasonic power is 200-400 W.

[0018] Preferably, the hydrogen content in the hydrogen-rich water is 1.6-4.6 mg / L.

[0019] The second aspect of the present invention provides a method for preparing the above-mentioned composition for preventing and treating acne, comprising the following steps:

[0020] Hydrogen-rich water, peach leaf-eucommia composite extract, vitamin E and vitamin C are added to aloe vera gel, and ultrasonic dispersion is performed for 2-4 hours to obtain a composition for preventing and treating acne.

[0021] The third aspect of the present invention provides use of the above-mentioned composition for preventing and treating acne in the preparation of a product for treating and treating acne.

[0022] Analysis of the main ingredients:

[0023] Peach leaf-Eucommia complex extract: Peach leaves are rich in polyphenol compounds, and Eucommia contains a variety of active ingredients such as chlorogenic acid and Eucommia steroids, which have antibacterial, anti-inflammatory and antioxidant functions, and are effective in fighting Propionibacterium acnes and reducing skin inflammation.

[0024] Vitamin E: As a powerful antioxidant, vitamin E can neutralize free radicals, protect skin cells from oxidative damage, and promote skin healing. At the same time, it can also regulate the skin's immune response, reduce the intensity of inflammatory response, and synergize with peach leaf-eucommia compound extract to exert anti-inflammatory effects.

[0025] Aloe vera gel: Aloe vera itself has soothing and anti-inflammatory effects, can repair the skin barrier, and help maintain the skin's moisture balance, alleviating skin irritation caused by acne. The addition of aloe vera gel can improve the moisturizing and soothing ability of the composition and enhance the overall skin care effect.

[0026] Hydrogen-rich water: The hydrogen in hydrogen-rich water has a strong antioxidant capacity, which can quickly neutralize free radicals in the skin and protect skin cells from oxidative damage. Hydrogen penetrates into skin cells and synergizes with vitamin E and other active ingredients to enhance the antioxidant and anti-inflammatory effects. In addition, hydrogen-rich water can increase the solubility of the extract, making the active ingredients more easily absorbed by the skin, further enhancing their effects.

[0027] Beneficial effects of the present invention:

[0028] 1. The present invention compounds peach leaf-eucommia composite extract, vitamin E, aloe vera gel, and hydrogen-rich water, and the components complement each other, and the functions of anti-oxidation, anti-inflammatory, and moisturizing are fully exerted, which can not only effectively prevent and treat acne, but also promote skin healing and repair. Different components form a multi-level synergistic effect by inhibiting bacteria, reducing inflammation, protecting skin cells, and enhancing skin barrier function. The synergistic effect of the peach leaf-eucommia composite extract and other components improves the inhibitory ability of three common pathogens of acne and reduces skin swelling and inflammation.

[0029] 2. By combining enzymatic hydrolysis with fermentation technology, the content of active ingredients in plant extracts is significantly increased, and their antibacterial and anti-inflammatory effects are enhanced. Ultrasonic extraction technology further accelerates the release of active ingredients, making the extracts easier to absorb, and improving the overall biological activity and product stability.

[0030] 3. The present invention adopts natural ingredients, avoids the side effects of antibiotics, and the product is mild and non-irritating, suitable for long-term use, especially for sensitive skin susceptible to acne. DETAILED DESCRIPTION

[0031] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0032] As described in the background technology, acne is a complex skin disease, and its pathogenesis involves multiple factors such as excessive secretion of sebaceous glands, keratinization of hair follicle sebaceous gland ducts, and excessive proliferation of Propionibacterium acnes. Existing anti-acne treatment methods usually rely on antibiotics and topical medications, but these treatments are often accompanied by problems such as skin irritation, microbial imbalance and drug resistance. Based on this, the development of a natural anti-acne composition with good efficacy and few side effects has become a research focus. In recent years, plant-derived active substances have shown great application potential in anti-acne due to their diverse biological activities and high safety.

[0033] In order to overcome the deficiencies in the prior art, the present invention provides a composition for preventing and treating acne, which can effectively inhibit three common acne pathogens, reduce skin inflammation, and alleviate swelling through the synergistic effect of peach leaf-eucommia composite extract, vitamin E, aloe gel and hydrogen-rich water.

[0034] In order to enable those skilled in the art to more clearly understand the technical solution of the present application, the technical solution of the present application will be described in detail below in conjunction with specific embodiments.

[0035] The test materials used in the examples of the present invention are all conventional test materials in the art and can be purchased through commercial channels.

[0036] The strain number of Lactobacillus rhamnosus used in the present invention is CGMCC1.12734, which was purchased from the General Microbiological Center of China National Microbiological Culture Collection Administration; the strain number of Bifidobacterium longum subsp.longum is CGMCC 1.3049, which was purchased from the General Microbiological Center of China National Microbiological Culture Collection Administration; the strain number of Saccharomyces cerevisiae used in the present invention is CGMCC 2.3866.

[0037] The strain preservation number of Propionibacterium acnes used in the present invention is CCTCCAB 2011020, which was purchased from the China Center for Type Culture Collection; the strain preservation number of Staphylococcus aureus used is CCTCC AB 2018158, which was purchased from the China Center for Type Culture Collection; the strain preservation number of Staphylococcus epidermidis used is CCTCC AB 2018375, which was purchased from the China Center for Type Culture Collection.

[0038] The pectinase used in the present invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with the product number S10007-5g.

[0039] The cellulase used in the present invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with the product number of S10041-5g.

[0040] The vitamin E used in the present invention was purchased from Shanghai Yuanye Biotechnology Co., Ltd. with a product number of S13016-25g and a purity of 50%.

[0041] The aloe vera gel used in the present invention was purchased from Guangzhou Xiya Biotechnology Co., Ltd., with a purity of 98% and a CAS number of 85507-69-3.

[0042] The peach leaf-eucommia composite extract used in the present invention is prepared by the following method:

[0043] (1) Peach leaves and Eucommia bark are crushed and mixed in a mass ratio of 5:4 to obtain a mixture, distilled water 15 times the mass of the mixture and a composite enzyme 0.5-1% of the mass of the mixture are added to the mixture, and after mixing, enzymolysis is carried out at 50° C. for 3 hours to obtain an enzymolysis solution;

[0044] The complex enzyme is composed of cellulase and pectinase in a mass ratio of 3:2;

[0045] (2) adding 10% of the volume of the mixed seed solution to the enzymatic hydrolysate for fermentation at 37° C. for 48 h to obtain a fermentation solution;

[0046] In the mixed seed solution, the volume ratio of Lactobacillus rhamnosus seed solution, Bifidobacterium longum seed solution and Saccharomyces cerevisiae seed solution was 4:5:3; the number of viable bacteria in the Lactobacillus rhamnosus seed solution was 1.2×10 8 cfu / ml; the viable count of Bifidobacterium longum seed solution is 2×10 8 cfu / ml; the number of viable bacteria in the Saccharomyces cerevisiae seed solution was 2.4×10 8 cfu / ml;

[0047] (3) The fermentation liquid was ultrasonically extracted at 300 W for 30 min, the supernatant was filtered, the supernatant was vacuum concentrated, and freeze-dried to obtain a peach leaf-eucommia composite extract.

[0048] Example 1: Preparation of a composition for preventing and treating acne:

[0049] 1. Raw material composition (parts by weight): 6 parts of peach leaf-eucommia composite extract, 1 part of vitamin E, 8 parts of aloe vera gel, 70 parts of hydrogen-rich water;

[0050] 2. Preparation steps:

[0051] Hydrogen-rich water, peach leaf-eucommia composite extract, vitamin E and vitamin C were added to aloe vera gel, and ultrasonic dispersion was performed for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water was 2.8 mg / L.

[0052] Example 2: Preparation of a composition for preventing and treating acne:

[0053] 1. Raw material composition (by mass): 9 parts of peach leaf-eucommia composite extract, 2 parts of vitamin E, 11.5 parts of aloe vera gel, 77.5 parts of hydrogen-rich water;

[0054] 2. Preparation steps:

[0055] Hydrogen-rich water, peach leaf-eucommia composite extract, vitamin E and vitamin C were added to aloe vera gel, and ultrasonic dispersion was performed for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water was 2.8 mg / L.

[0056] Example 3: Preparation of a composition for preventing and treating acne:

[0057] 1. Raw material composition (parts by weight): 12 parts of peach leaf-eucommia composite extract, 3 parts of vitamin E, 15 parts of aloe vera gel, 85 parts of hydrogen-rich water;

[0058] 2. Preparation steps:

[0059] Hydrogen-rich water, peach leaf-eucommia composite extract, vitamin E and vitamin C were added to aloe vera gel, and ultrasonic dispersion was performed for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water was 2.8 mg / L.

[0060] Comparative Example 1:

[0061] 1. Raw material composition (by mass): 6 parts of peach leaf extract, 1 part of vitamin E, 8 parts of aloe vera gel, 70 parts of hydrogen-rich water;

[0062] 2. Preparation steps:

[0063] (1) Peach leaf extract is prepared by the following method:

[0064] Peach leaves are crushed, distilled water 15 times the mass of the peach leaf powder and a complex enzyme 0.5-1% of the mass of the peach leaf powder are added to the peach leaf powder, and after mixing evenly, an enzymolysis reaction is carried out at 50° C. for 3 hours to obtain an enzymolysis solution;

[0065] The complex enzyme is composed of cellulase and pectinase in a mass ratio of 3:2;

[0066] Adding 10% of the volume of the mixed seed solution to the enzymatic hydrolysate for fermentation at 37° C. for 48 hours to obtain a fermentation solution;

[0067] In the mixed seed solution, the volume ratio of Lactobacillus rhamnosus seed solution, Bifidobacterium longum seed solution and Saccharomyces cerevisiae seed solution was 4:5:3; the number of viable bacteria in the Lactobacillus rhamnosus seed solution was 1.2×10 8 cfu / ml; the viable count of Bifidobacterium longum seed solution is 2×10 8 cfu / ml; the number of viable bacteria in the Saccharomyces cerevisiae seed solution was 2.4×10 8 cfu / ml;

[0068] The fermentation liquid was ultrasonically extracted at 300 W for 2 h, the supernatant was filtered, the supernatant was vacuum concentrated, and freeze-dried to obtain a peach leaf extract.

[0069] (2) Add hydrogen-rich water, peach leaf extract, vitamin E and vitamin C to aloe vera gel, and ultrasonically disperse for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water is 2.8 mg / L.

[0070] Comparative Example 2:

[0071] 1. Raw material composition (by mass): 6 parts of Eucommia ulmoides extract, 1 part of vitamin E, 8 parts of aloe vera gel, 70 parts of hydrogen-rich water;

[0072] 2. Preparation steps:

[0073] (1) The Eucommia ulmoides extract is prepared by the following method:

[0074] The bark of Eucommia ulmoides is crushed, and distilled water of 15 times the mass of the bark powder of Eucommia ulmoides and a composite enzyme of 0.5-1% of the mass of the bark powder of Eucommia ulmoides are added to the bark powder of Eucommia ulmoides, and after being mixed evenly, an enzymolysis reaction is carried out at 50° C. for 3 hours to obtain an enzymolysis solution;

[0075] The complex enzyme is composed of cellulase and pectinase in a mass ratio of 3:2;

[0076] Adding 10% of the volume of the mixed seed solution to the enzymatic hydrolysate for fermentation at 37° C. for 48 hours to obtain a fermentation solution;

[0077] In the mixed seed solution, the volume ratio of Lactobacillus rhamnosus seed solution, Bifidobacterium longum seed solution and Saccharomyces cerevisiae seed solution was 4:5:3; the number of viable bacteria in the Lactobacillus rhamnosus seed solution was 1.2×10 8 cfu / ml; the viable count of Bifidobacterium longum seed solution is 2×10 8 cfu / ml; the number of viable bacteria in the Saccharomyces cerevisiae seed solution was 2.4×10 8 cfu / ml;

[0078] The fermentation liquid was ultrasonically extracted at 300 W for 2 h, the supernatant was filtered, the supernatant was vacuum concentrated, and freeze-dried to obtain the Eucommia ulmoides extract.

[0079] (2) Add hydrogen-rich water, Eucommia ulmoides extract, vitamin E and vitamin C to aloe vera gel, and ultrasonically disperse for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water is 2.8 mg / L.

[0080] Comparative Example 3:

[0081] 1. Raw material composition (parts by weight): 1 part of vitamin E, 8 parts of aloe vera gel, 70 parts of hydrogen-rich water;

[0082] 2. Preparation steps:

[0083] Hydrogen-rich water, vitamin E and vitamin C were added to aloe vera gel and ultrasonically dispersed for 2 hours to obtain a composition for preventing and treating acne; the hydrogen content in the hydrogen-rich water was 2.8 mg / L.

[0084] Test Example 1: Antibacterial effect test of the composition for preventing and treating acne on Propionibacterium acnes:

[0085] 1. Test method:

[0086] Referring to the step 2.3.6 Evaluation of the antibacterial activity of Sophora flavescens extract in the document "Study on the Extraction and Antibacterial Activity of Matrine" (Guo Chaowan et al. Guangdong Chemical Industry, 2021), experiments were conducted on the compositions for preventing and treating acne of Example 1 and Comparative Examples 1-3. The test bacteria were Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis.

[0087] Use a micropipette to draw 100uL of 10 6 CFU / mL of the test strain suspension to the surface of the agar medium, spread it evenly with a sterile coating rod, cover the plate, and dry it at room temperature for 5-8 minutes. Gently use sterile tweezers to stand a sterile Oxford cup (8mm in diameter) on the surface of the culture medium, dilute the acne prevention and treatment composition of Example 1 and Comparative Examples 1-3 with 20% ethanol to a concentration of 50mg / mL sample solution, and use a micropipette to draw 200uL of the sample solution into the Oxford cup. The sample solution is set in 3 parallels, and 20% ethanol is used as a negative control. The culture dish is placed in an incubator at 37°C and the results are observed after 24h. The diameter of the inhibition zone is determined by the cross method as the standard.

[0088] The strength of the antibacterial ability can be expressed by the diameter of the antibacterial zone. The antibacterial zone result judgment standard in the "Technical Specifications for Disinfection" is adopted: ≤10mm is low sensitivity; 10-14 is moderate sensitivity; 15-20 is high sensitivity; ≥20mm is extremely sensitive.

[0089] 2. Test results:

[0090] Table 1 Diameter of inhibition zone of the composition for preventing and treating acne against the main pathogenic bacteria of acne (mm)

[0091] Treatment Group Propionibacterium acnes Staphylococcus aureus Staphylococcus epidermidis Example 1 29.7 21.5 22.7 Comparative Example 1 21.6 15.69 16.54 Comparative Example 2 19.2 13.54 14.63 Comparative Example 3 12.5 10.64 11.25

[0092] As shown in Table 1, Example 1 compounded the peach leaf-eucommia composite extract, vitamin E, aloe vera gel and hydrogen-rich water, and showed extremely sensitive antibacterial effects on three common acne pathogens, which has good prospects in preventing and treating acne.

[0093] Test Example 2: Effect of the Composition for Preventing and Treating Acne on Rat Acne Model:

[0094] 1. Model construction: Refer to the literature "Study on the improvement of inflammatory response of acne model rats with Xiaocuo decoction. Chinese Medicinal Materials, 2022" 1.2.1 to construct a rat auricle acne model:

[0095] 1 mL of oleic acid was applied to the opening of the right ear duct of the rats, and 100 μL of Propionibacterium acnes (6×10 7 CFU / mL) for 14 consecutive days. If the auricle at the modeling site is significantly thickened, red, swollen, hardened with nodules, and sebum flakes appear on the skin surface, the modeling is successful.

[0096] 2. Experimental groups:

[0097] A total of 80 rats with auricular acne model were selected and randomly divided into 4 groups, with 20 rats in each group.

[0098] The 4 groups of auricular acne model rats were given medication as soon as the model was successfully established, and the medication was continued for 21 days. The medication conditions of the different treatment groups are as follows:

[0099] T1 treatment group: The acne prevention and treatment composition prepared in Example 1 was applied to the right ears of rats once in the morning and once in the evening.

[0100] 0.1g each time;

[0101] T2 treatment group: The acne prevention and treatment composition prepared in Comparative Example 1 was applied to the right ears of mice once in the morning and once in the evening.

[0102] 0.1g each time;

[0103] T3 treatment group: The acne prevention and treatment composition prepared in Comparative Example 2 was applied to the right ears of the mice once in the morning and once in the evening.

[0104] 0.1g each time;

[0105] T4 treatment group: The composition for preventing and treating acne prepared in Comparative Example 3 was applied to the right ears of mice, once in the morning and once in the evening, 0.1 g each time.

[0106] In addition, 20 normal control group rats were selected and applied with equal amount of normal saline every morning and evening.

[0107] 3. Detection indicators:

[0108] 3.1 Rat ear swelling inhibition rate:

[0109] After the model was established, the thickness of the right ear auricle of the rats in the T1-T4 treatment groups was measured and recorded as the auricle thickness after model establishment. During the drug treatment period, the ear skin of the rats in the observation group was improved. After the drug administration, the thickness of the right ear auricle of the rats in the treatment group was measured and recorded as the auricle thickness after treatment, and the inhibition rate of auricle swelling in the rats was calculated.

[0110] Swelling inhibition rate % = (auricle thickness after modeling - auricle thickness after treatment) / auricle thickness after modeling × 100%.

[0111] 3.2 Enzyme-linked immunosorbent assay (ELISA) to detect serum TNF-α and IL-6 levels:

[0112] After the last administration, all rats were fasted but not watered for 24 hours, anesthetized with 10% chloral hydrate, and the abdominal aorta was isolated for blood collection. The blood was left at room temperature for more than 2 hours, centrifuged at 3500r / min, 4°C for 15 minutes, and the supernatant was collected. All reagents in the ELISA kit were left at room temperature for more than 30 minutes, and the TNF-α and IL-6 contents were tested in strict accordance with the instructions of the kit.

[0113] Table 2 Effects of different treatments on rats

[0114]

[0115]

[0116] As can be seen from Table 2, the anti-acne composition prepared in Example 1 has the highest inhibition rate on the swelling of the rat auricle, and the TNF-α content and IL-6 content are basically the same as those in the normal control group, indicating that the anti-acne composition prepared in Example 1 has a significant anti-inflammatory effect.

[0117] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A composition for preventing and treating acne, characterized in that: The invention comprises the following components in parts by weight: 6-12 parts of peach leaf-eucommia ulmoides composite extract, 1-3 parts of vitamin E, 8-15 parts of aloe vera gel and 70-85 parts of hydrogen-rich water.

2. The composition for preventing and treating acne according to claim 1, characterized in that: The peach leaf-eucommia composite extract is prepared by the following method: (1) Peach leaves and Eucommia bark are crushed and mixed in a mass ratio of (3-7): (3-5) to obtain a mixture, distilled water and a complex enzyme consisting of cellulase and pectinase are added to the mixture, and after mixing, enzymolysis reaction is performed to obtain an enzymolysis solution; (2) adding a mixed seed liquid consisting of a Lactobacillus rhamnosus seed liquid, a Bifidobacterium longum seed liquid, and a Saccharomyces cerevisiae seed liquid to the enzymatic hydrolyzate for fermentation to obtain a fermentation liquid; (3) Ultrasonic extraction of the fermentation liquid for 10-45 min, filtering the extract to obtain the supernatant, concentrating and drying to obtain a peach leaf-eucommia composite extract.

3. The composition for preventing and treating acne according to claim 1, characterized in that: In step (1), the amount of distilled water added is 10-15 times the mass of the mixture; the amount of complex enzyme added is 0.5-1% of the mass of the mixture, and the complex enzyme is a mixture of cellulase and pectinase in a mass ratio of (2-4): (1-3).

4. The composition for preventing and treating acne according to claim 1, characterized in that: In step (1), the temperature of the enzymatic hydrolysis reaction is 40-60° C. and the time is 2-4 h.

5. The composition for preventing and treating acne according to claim 1, characterized in that: In step (2), the amount of the mixed seed solution added is 5%-10% of the volume of the enzymatic hydrolyzate; in the mixed seed solution, the volume ratio of the Lactobacillus rhamnosus seed solution, the Bifidobacterium longum seed solution and the Saccharomyces cerevisiae seed solution is (3-5): (4-6): (2-4); The number of viable bacteria in Lactobacillus rhamnosus seed solution is ≥1.2×10 8 cfu / ml; the number of viable bacteria in the seed solution of Bifidobacterium longum ≥ 2×10 8 cfu / ml; the number of viable bacteria in the brewer's yeast seed solution is ≥2.4×10 8 cfu / ml.

6. The composition for preventing and treating acne according to claim 1, characterized in that: In step (2), the fermentation temperature is 30-37° C. and the fermentation time is 36-60 h.

7. The composition for preventing and treating acne according to claim 1, characterized in that: In step (3), the extraction time of ultrasonic extraction is 15-30 minutes, and the ultrasonic power is 200-400W.

8. The composition for preventing and treating acne according to claim 1, characterized in that: The hydrogen-rich water has a hydrogen content of 1.6-4.6 mg / L.

9. The method for preparing the composition for preventing and treating acne according to any one of claims 1 to 8, characterized in that: The following steps are involved: Hydrogen-rich water, peach leaf-eucommia composite extract, vitamin E and vitamin C are added to aloe vera gel, and ultrasonic dispersion is performed for 2-4 hours to obtain a composition for preventing and treating acne.

10. Use of the composition for preventing and treating acne according to any one of claims 1 to 8 in the preparation of products for preventing and treating acne.

Images