Chinese and western compound composition as well as preparation method and application thereof

Abstract

The invention relates to a Chinese and western compound composition for resisting internal and external parasites of animals as well as a preparation method and application thereof, and belongs to the field of veterinary drugs. The Chinese and western compound composition is prepared from betel nut extract, pumpkin seed extract, antifebrile dichroa extract, sweet wormwood herb extract, radix stemonae extract, radix astragali extract, vitamin A, vitamin E and ivermectin. The Chinese and western compound composition disclosed by the invention has the effects of clearing heat and eliminating dampness, killing parasites and removing food retention, expelling parasites for purgation, promoting drug absorption, enhancing the immunity of the organism, expanding the insect-resistant spectrum and reducing the generation of drug resistance, is low in toxic and side effects, and has the effect superior to that of a chemical medicine single-prescription preparation or a pure traditional Chinese medicine preparation. The traditional Chinese medicine composition has very good prevention and treatment effects on common parasites in and out of animal bodies.

Description

Technical Field

[0001] The present invention relates to the field of veterinary medicine, and in particular to a Chinese and Western medicine compound composition for resisting internal and external parasites for animals, and a preparation method and application thereof. Background Art

[0002] Animal parasitic diseases are the most widespread and diverse animal diseases in the world, causing huge economic losses worldwide. Parasitic diseases of ruminants are common diseases in cattle and sheep breeding. They are chronic digestive diseases caused mainly by mosquitoes, flies, lice, scabies, nematodes, tapeworms, coccidia, and blood protozoa. They manifest as emaciation, stunted development, and decreased production performance in animals, causing great economic losses to cattle and sheep farmers.

[0003] At present, the Chinese herbal antiparasitic drugs on the market mainly include Quqiu Zhili Mixture, Chicken Coccidia Powder, Sanwei Kangqiu Granules, Wuwei Changqing Granules, Qinghao Changshan Granules, Quchong Powder, etc., which are mainly used to prevent coccidiosis. Only Quchong Powder is used to kill livestock and poultry nematodes, tapeworms, flukes and mites, ticks, lice, etc. Chinese herbal antiparasitic drugs are generally used to prevent parasitic diseases. When used as therapeutic drugs, they take effect slowly and require large dosages. However, Chinese herbal antiparasitic drugs are safe, environmentally friendly, and not prone to drug resistance. They play a vital role in the prevention and treatment of animal parasitic diseases and are important alternative drugs.

[0004] Ivermectin is a widely recognized antiparasitic drug with broad spectrum, high efficiency, safety and low residue. It has a broad antiparasitic spectrum, is cheap and low cost, and is suitable for large-scale use in farms. It mainly blocks the transmission of nerve signals, paralyzes nerves, and causes the death of the worm body. It has a repelling effect on nematodes (Osterna, Trichostrongylus, Neck nematodes, Trichocephalus, Bunostomum, Gastroenteritis, Ascaris, Whipworm) and external parasites (mosquitoes, flies, lice, mites, ticks, fleas, etc.). In particular, it plays an irreplaceable role in controlling arthropod external parasitic diseases and gastrointestinal nematode diseases. It is the most ideal drug for preventing and treating livestock parasitic diseases, but it is ineffective against trematodes, tapeworms, coccidia, and blood protozoa. Therefore, the development of compound drugs composed of ivermectin and traditional Chinese medicine has development prospects. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a Chinese and Western compound composition for animal antibodies against internal and external parasites

[0006] The Chinese and Western compound composition is prepared from 20-35 parts of betel nut extract, 35-45 parts of pumpkin seed extract, 20-35 parts of dactylum extract, 20-35 parts of artemisia annua extract, 20-40 parts of stemona extract, 35-50 parts of astragalus extract, 20-50 parts of vitamin A, 10-40 parts of vitamin E and 0.25-0.8 parts of ivermectin.

[0007] Furthermore, the Chinese and Western compound composition is prepared from 25-35 parts of betel nut extract, 40-45 parts of pumpkin seed extract, 28-35 parts of radix changshanii extract, 25-30 parts of artemisia annua extract, 30-40 parts of stemonae extract, 40-50 parts of astragalus extract, 25-50 parts of vitamin A, 25-40 parts of vitamin E, and 0.5-0.8 parts of ivermectin.

[0008] Furthermore, the Chinese and Western compound composition is prepared from 28-35 parts of betel nut extract, 40-45 parts of pumpkin seed extract, 30-35 parts of radix changshanii extract, 27-30 parts of artemisia annua extract, 35-40 parts of stemonae extract, 40-45 parts of astragalus extract, 30-50 parts of vitamin A, 30-40 parts of vitamin E, and 0.5-0.8 parts of ivermectin.

[0009] Furthermore, the Chinese and Western compound composition is prepared from 35 parts of betel nut extract, 45 parts of pumpkin seed extract, 35 parts of radix changshanii extract, 30 parts of artemisia annua extract, 40 parts of stemonae extract, 45 parts of astragalus extract, 50 parts of vitamin A, 40 parts of vitamin E and 0.8 parts of ivermectin.

[0010] The present invention also provides a method for preparing the Chinese and Western compound composition for animals to fight internal and external parasites, which is characterized in that the preparation is carried out according to the following steps:

[0011] (1) Mix and stir the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filter the mixture through a 200-mesh sieve after stirring evenly, and set aside;

[0012] (2) Mix vitamin A, vitamin E and ivermectin evenly and set aside;

[0013] (3) The mixtures obtained in step (1) and step (2) are mixed uniformly, and soluble starch, surfactant, cosolvent, etc. are added, and then flavoring agents and preservatives are added, and mixed thoroughly to obtain a soluble powder.

[0014] The present invention also provides the use of the Chinese and Western compound composition of antibodies for animals against internal and external parasites in the preparation of medicines for preventing and treating internal and external parasites in animals.

[0015] Furthermore, the animal is a ruminant.

[0016] Furthermore, the ruminant is a cattle.

[0017] Furthermore, the external parasite is Dermestidae.

[0018] Furthermore, the internal parasites are nematodes and / or tapeworms.

[0019] The advantages and beneficial effects of the medicine of the present invention are:

[0020] Each drug component and its dosage of the present invention are obtained by repeated verification through a large number of experiments. Traditional Chinese medicine believes that worm syndrome refers to a disease syndrome caused by worms parasitic in the intestines, which generally has symptoms such as hair burning and hanging, not growing after fullness or stool disorder. And parasites parasitizing in the body are often accompanied by weak spleen deficiency and loss of positive energy, so drug treatment is carried out with the principles of killing insects and eliminating accumulation, regulating qi and strengthening the spleen, and attacking and supplementing as the treatment principles for both internal and external treatment. In the prescription of the present invention, Artemisia annua and Radix Dichroae are used as monarch drugs to clear away heat and dampness, stop malaria and kill insects; Areca nut, pumpkin seeds, and Stemona are used as ministerial drugs to kill insects and eliminate accumulation, and to raise yang and lift depression. Astragalus is used as an adjuvant drug to increase immunity, support poison and promote muscle regeneration. All drugs are used together to play the effects of clearing heat and dampness, killing insects and eliminating accumulation, strengthening the spleen and stomach, and raising yang energy. While expelling parasites, it also enhances the body's immunity, improves the animal's eating conditions, and improves feed conversion rate.

[0021] The Chinese and Western compound composition of the present invention combines ivermectin, a Chinese medicinal extract for resisting internal and external parasites, and a vitamin-based drug to develop an anti-animal parasitic disease drug, which not only improves the anti-internal and external parasite effect and expands the anti-parasitic spectrum, but also slows down the generation of drug resistance, thus providing a reliable solution for treating internal and external parasitic diseases in animals.

[0022] Clinical trials have shown that the Chinese and Western compound composition of the present invention has a good preventive and therapeutic effect on parasites such as mosquitoes, flies, lice, ticks, mites, coccidia, various nematodes, and tapeworms inside and outside animals. The medicine of the present invention is a Chinese and Western compound with synergistic effects. The medicine of the present invention has a wider antiparasitic spectrum than single chemical preparations or pure Chinese medicine preparations, and has a good antiparasitic effect. The vitamin drugs and antiparasitic drugs contained in the Chinese and Western compound composition of the present invention have a synergistic effect, enhance the ability of antiparasitic drugs to eliminate parasites, and improve the defense ability of animal bodies against parasitic diseases. No poisoning reactions and adverse reactions were found in the three-fold dose of the Chinese and Western compound composition. Ivermectin and Chinese herbal medicine extracts in the formula are commonly used antiparasitic drugs in clinical practice, and the dosage of the Chinese and Western compound preparation is less than that of traditional Chinese medicine, which can achieve better prevention and control effects at a relatively low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The results are the comparison of relative weight gain rates of different dosage groups. DETAILED DESCRIPTION

[0024] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0025] Example 1

[0026] Weigh the following raw materials by weight: 30 parts of vitamin A, 25 parts of vitamin E, 30 parts of betel nut extract, 40 parts of pumpkin seed extract, 30 parts of radix changshanii extract, 25 parts of artemisia annua extract, 30 parts of stemonae extract, 40 parts of astragalus extract, and 0.5 parts of ivermectin. Prepare and use according to the following method.

[0027] Process the weighed medicinal materials according to the following steps:

[0028] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0029] (2) Mix vitamin A, vitamin E and ivermectin uniformly to obtain substance B.

[0030] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0031] Example 2

[0032] Weigh the following raw materials by weight: 25 parts of vitamin A, 30 parts of vitamin E, 28 parts of betel nut extract, 35 parts of pumpkin seed extract, 28 parts of changshan extract, 27 parts of artemisia annua extract, 35 parts of stemona extract, 35 parts of astragalus extract, and 0.5 parts of ivermectin. Prepare and use according to the following method.

[0033] Process the weighed medicinal materials according to the following steps:

[0034] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0035] (2) Mix vitamin A, vitamin E and ivermectin uniformly to obtain substance B.

[0036] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0037] Example 3

[0038] Weigh the following raw materials by weight: 50 parts of vitamin A, 40 parts of vitamin E, 35 parts of betel nut extract, 45 parts of pumpkin seed extract, 35 parts of radix changshanii extract, 30 parts of artemisia annua extract, 40 parts of stemonae extract, 45 parts of astragalus extract, and 0.8 parts of ivermectin. Prepare and use according to the following method.

[0039] Process the weighed medicinal materials according to the following steps:

[0040] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0041] (2) mixing vitamin A, vitamin E and ivermectin uniformly to obtain substance B;

[0042] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0043] Example 4

[0044] Weigh the following raw materials by weight: 30 parts of vitamin A, 10 parts of vitamin E, 35 parts of betel nut extract, 35 parts of pumpkin seed extract, 35 parts of changshan extract, 20 parts of artemisia annua extract, 20 parts of stemona extract, 45 parts of astragalus extract, and 0.8 parts of ivermectin. Prepare and use according to the following method.

[0045] Process the weighed medicinal materials according to the following steps:

[0046] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0047] (2) mixing vitamin A, vitamin E and ivermectin uniformly to obtain substance B;

[0048] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0049] Example 5

[0050] Weigh the following medicinal raw materials according to weight ratio: 20 parts of vitamin A, 10 parts of vitamin E, 25 parts of areca nut extract, 35 parts of pumpkin seed extract, 20 parts of changshan extract, 20 parts of artemisia annua extract, 20 parts of stemona extract, 35 parts of astragalus extract, and 0.25 parts of ivermectin.

[0051] Process the weighed medicinal materials according to the following steps:

[0052] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0053] (2) mixing vitamin A, vitamin E and ivermectin uniformly to obtain substance B;

[0054] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0055] Example 6

[0056] Weigh the following raw materials by weight: 20 parts of vitamin A, 30 parts of vitamin E, 20 parts of betel nut extract, 40 parts of pumpkin seed extract, 20 parts of changshan extract, 25 parts of artemisia annua extract, 20 parts of stemon extract, 50 parts of astragalus extract, and 0.25 parts of ivermectin. Process the weighed medicinal materials according to the following steps:

[0057] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0058] (2) mixing vitamin A, vitamin E and ivermectin uniformly to obtain substance B;

[0059] (3) Mix substance A and substance B evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0060] Comparative Example 1

[0061] Weigh the following raw materials by weight: 30 parts of Areca nut extract, 40 parts of Cucurbita chinensis extract, 30 parts of Dichroa chinensis extract, 25 parts of Artemisia annua extract, 30 parts of Stemona extract, and 40 parts of Astragalus extract. Prepare and use according to the following method.

[0062] Process the weighed medicinal materials according to the following steps:

[0063] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0064] (2) Add soluble starch, surfactant, cosolvent, etc. to substance A, then add flavoring agent and preservative, mix well, and obtain soluble powder.

[0065] Comparative Example 2

[0066] Weigh the following raw materials by weight: 30 parts of betel nut extract, 40 parts of pumpkin seed extract, 30 parts of radix changshanii extract, 25 parts of artemisia annua extract, 30 parts of stemonae extract, 40 parts of astragalus extract, and 0.5 parts of ivermectin. Prepare and use according to the following method.

[0067] Process the weighed medicinal materials according to the following steps:

[0068] (1) Mixing and stirring the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filtering the mixture through a 200-mesh sieve to obtain substance A;

[0069] (2) Mix substance A and ivermectin evenly, add soluble starch, surfactant, cosolvent, etc., and then add flavoring agent and preservative, mix well to obtain soluble powder.

[0070] Comparative Example 3

[0071] Weigh the following drug raw materials according to weight ratio: 0.57 parts of ivermectin, 113 parts of starch, 0.45 parts of artemisinin, 0.9 parts of liquidambar formosana oil, 0.3 parts of schizophrenia extract, and 0.5 parts of ginkgo exocarp extract.

[0072] After the extract is dried and crushed, ivermectin is added and mixed evenly, soluble starch, surfactant, cosolvent, etc. are added, and then flavoring agents and preservatives are added and mixed well to obtain soluble powder.

[0073] Comparative Example 4

[0074] Weigh the following drug raw materials according to weight ratio: 1 part of ivermectin, 1 part of vitamin D3, 2 parts of vitamin E, 0.1 part of betel nut extract, 0.1 part of thunder ball extract, 0.1 part of ginkgo leaf extract, 0.2 part of stemona extract, and 0.5 part of agrimony root and bud extract.

[0075] The above-mentioned betel nut extract, thunder ball extract, ginkgo leaf extract, stemona extract, and agrimony root and bud extract are placed in a blender and stirred and mixed. Vitamin D3, vitamin E, and ivermectin are added and stirred evenly, and then soluble starch, surfactant, cosolvent, etc. are added, and then flavoring agents and preservatives are added and mixed well to obtain soluble powder.

[0076] Comparative Example 5

[0077] Weigh the following medicinal raw materials according to the weight ratio: 500 g of Quisqualis chinensis, 500 g of Areca catechu, and 10 g of ivermectin.

[0078] Weigh the above-mentioned weight portions of Quisqualis Creeper and Areca Nut, mix and extract, crush and sieve the extract, add ivermectin and mix evenly, add soluble starch, surfactant, cosolvent, etc., add flavoring agent, preservative, mix well to obtain soluble powder.

[0079] Comparative Example 6

[0080] Weigh the following medicinal raw materials according to weight ratio: 300 parts of Artemisia annua, 250 parts of Areca catechu, 100 parts of Pumpkin seeds, 80 parts of Astragalus, and 50 parts of Sophora flavescens.

[0081] The extracts are obtained by extracting the above-mentioned Chinese medicinal components, and after being mixed evenly, soluble starch, surfactants, cosolvents, etc. are added, and then flavoring agents and preservatives are added, and mixed thoroughly to obtain soluble powders.

[0082] Comparative Example 7

[0083] Weigh the following medicinal raw materials according to the weight ratio: 55g of Quisqualis chinensis, 35g of Dichroa chinensis, 35g of Cyperus rotundus, 25g of Prunus mume, 25g of Artemisia annua, 15g of Honeysuckle, 15g of cinnamon bark, 15g of ginseng, 15g of Angelica sinensis, 12g of Areca nut, 12g of Talcum, 16g of Pumpkin Seed, 9g of Panax notoginseng, 9g of Gastrodia elata, and 5g of Astragalus.

[0084] The above-mentioned weight of drugs are mixed and extracted, the extract is dried and crushed, and then soluble starch, surfactant, cosolvent, etc. are added, and then flavoring agents and preservatives are added, and mixed thoroughly to obtain soluble powder.

[0085] Comparative Example 8

[0086] Weigh the following raw materials by weight: 20g of Artemisia annua, 30g of Dichroa chinensis, 10g of Pulsatilla chinensis, 10g of Sanguisorba officinalis, 10g of Angelica sinensis, 10g of Astragalus, 15g of Galla chinensis, 15g of Areca catechu, and 10g of Rhubarb. Crush the above Chinese medicines and pass them through a 800-mesh sieve, mix the sieved raw materials evenly to obtain the Chinese medicine composition mixture, extract the Chinese medicine mixture, dry and crush the extract, add soluble starch, surfactant, cosolvent, etc., add flavoring agent, preservative, and mix them well to obtain soluble powder.

[0087] Comparative Example 9

[0088] Weigh the following raw materials by weight: 30 parts of Artemisia annua, 25 parts of Dichroa, 10 parts of Agrimoniae, 10 parts of Sophora flavescens, 5 parts of Bupleurum, 5 parts of Astragalus, 5 parts of Licorice, 5 parts of Crataegus, 5 parts of Aucklandia lappa. Mix and extract the above Chinese medicinal components, dry and crush the extract, add soluble starch, surfactant, cosolvent, etc., then add flavoring agent, preservative, mix well, and obtain soluble powder.

[0089] Test Example 1

[0090] In order to evaluate the safety of this prescription, the safety of the drug in Example 1 of the present invention was evaluated on beef cattle. The specific test is as follows:

[0091] 1 Materials and Animals

[0092] 1.1 Trial Drugs

[0093] Embodiment 1 medicine of the present invention.

[0094] 1.2 Experimental animals

[0095] There are 24 beef cattle, 6 in each group, weighing about 150kg.

[0096] 2 Methods

[0097] 2.1 Grouping and Dosing

[0098] The experiment was divided into 4 groups: blank control group, 1-fold dose group (1g / 10kg), 3-fold dose group, and 5-fold dose group.

[0099] 2.2 Test methods

[0100] Beef cattle weighing about 150 kg were weighed 8 hours after being deprived of feed before the test. The drug was administered according to the body weight of each cattle. After administration, the cattle were observed for 7 days to see if there were any abnormalities in their mental state, breathing, feces, etc. After 7 days, each cattle was weighed and recorded.

[0101] 3 Results and analysis

[0102] Before and after the test, the relative weight gain rates of the control group, 1-fold dose group, 3-fold dose group, and 5-fold dose group were 100%, 99.9%, 101.2%, and 98.8%, respectively. There was no significant difference in the amount of food and water consumed by each group, and there was no abnormality in the mental state and feces, indicating that the drug safety prepared by the pharmaceutical composition of the present invention is good (see Figure 1 ).

[0103] Test Example 2

[0104] In order to verify the efficacy of the drug of the present invention, in July 2021, a certain breeding research center conducted an observation test on the control effect of the drug of the present invention on cattle external parasites - cow flies and other external parasites, and a control effect test on cattle internal nematodes and tapeworms. The results are as follows:

[0105] 1 Materials and animals

[0106] 1.1 Trial Drugs

[0107] The medicine prepared in Examples 1-6;

[0108] 1.2 Experimental animals

[0109] A total of 140 calves were selected from a naturally infected herd, with 20 calves in each group.

[0110] 2 Methods

[0111] 2.1 Grouping and Dosing

[0112] 140 calves were selected as experimental animals, with 20 calves in each group. Experimental group 1 was a control group without drug administration, which was not administered but was orally administered with the same volume of normal saline; experimental groups 2 to 7 were drug groups of drug examples 1 to 6 of the present invention, respectively, and were orally administered with 1 g / 10 kg body weight. Each group was raised separately under the same feeding conditions.

[0113] 2.2 Trial on the treatment of cowflies

[0114] 2.2.1 Test methods

[0115] The drug test group was given the drug according to body weight. The weight calculation formula is body weight (kg) = [chest circumference (m) 2 ×soft italic (m) / 10800]×0.85. After the administration, the cattle were marked one by one and were still grazed in the grassland field. The cattle in the control group without the administration were given the same volume of physiological saline. The presence of visible parasites on the body surface of each cattle was counted 7 days, 14 days, and 30 days after the administration. The presence of tumor blisters and worm holes on the back skin of cattle in each group was checked 5 months and 7 months after the administration. The efficacy was judged according to the increase, decrease, and disappearance of tumor blisters and worm holes.

[0116] 2.2.2 Criteria for determining efficacy

[0117] The control effect on cow hide flies was calculated according to the following formula to evaluate the control effect.

[0118] Cure rate (%) = (number of positive cows in the control group - number of uncured cows after administration) / number of cows in the control group × 100%.

[0119] Antiparasitic rate (%) = (average number of blisters in the control group - average number of blisters in the test group) / average number of blisters in the control group × 100%.

[0120] 2.3 Determination of the control effect on external parasites

[0121] Judgment criteria: The animal is considered cured when there are no parasites visible to the naked eye on its body surface.

[0122] 2.4 Experiment on the control effect of nematodes and tapeworms in cattle

[0123] 2.4.1 Test methods

[0124] The drug test group was given the drug according to body weight. The formula for calculating body weight is body weight (kg) = [chest circumference (m)2 × soft italic (m) / 10800] × 0.85. After the drug was administered, the cattle were marked one by one, and they were still grazed with the group on the grassland after the drug administration. The cattle in the control group without drug administration were given the same volume of normal saline. On the day of drug administration (0 day, before drug administration) and on the 3rd, 7th, 14th and 21st days after drug administration, the rectum of each group of test cattle was collected for fecal worm egg count using the McMaster counting method to calculate the number of infections. After the drug administration, the feces was soaked and processed to accurately observe the eggs in the feces, and the types of parasites in the feces were observed using the saturated salt water flotation method. In addition, pay attention to whether there are yellow or white cylindrical egg-bearing segments in the feces.

[0125] 2.4.2 Criteria for determining efficacy

[0126] According to the changes in eggs (larvae) in the feces of each group of cattle before and after deworming, observe whether the clinical symptoms (diarrhea, weight loss, changes in fecal characteristics or vomiting, etc.) disappear, the reduction rate of nematodes and eggs in feces is ≥90%, and the physical signs of the test animals return to normal. The main statistics are the egg reduction rate and deworming rate, and the deworming effect is calculated according to the following formula:

[0127] Egg reduction rate (%) = (number of eggs per gram of feces before the test - number of eggs per gram of feces after the test) / number of eggs per gram of feces before the test × 100%;

[0128] Deworming rate (%) = (average infection intensity before the experiment - average infection intensity after the experiment) / average infection intensity before the experiment × 100%;

[0129] Relative anthelmintic rate (%) = (number of worms in the non-medication group - number of worms in the medication group) / number of worms in the non-medication group × 100%.

[0130] 3 Results and analysis

[0131] The results of the expulsion of internal and external parasites of Examples 1-6 are shown in Tables 1, 2, and 3. It can be seen from Tables 1 and 2 that the effects of Examples 3 and 4 on expulsion of external parasites are slightly better than those of Examples 5 and 6. It can be seen from Table 3 that the effects of the expulsion of internal and external parasites of Examples 1-4 are slightly better than those of Examples 5 and 6. The reason may be related to the amount of ivermectin and the content of traditional Chinese medicine in the examples. From the overall results, Examples 1-6 have uniform and good effects on expulsion of internal and external parasites.

[0132] Table 1 Test results of Examples 1-6 for expelling cow hide fly maggots for 5 and 7 months

[0133]

[0134] Table 2 Experimental results of Examples 1-6 for removing other parasites from cattle

[0135]

[0136] Table 3 Experimental results of removing digestive tract nematode eggs and tapeworms in Examples 1-6

[0137]

[0138] Test Example 3

[0139] To further verify the efficacy of the drug of the present invention, in March 2022, a test on the prevention and control effects of Example 1 and Comparative Examples 1-9 on nematodes and tapeworms in cattle was carried out at a cattle farm in Inner Mongolia Autonomous Region.

[0140] 1. Materials and Animals

[0141] 1.1 Trial Drugs

[0142] The drugs prepared in Example 1 and Comparative Examples 1-9 are 1% ivermectin injection (commercially available).

[0143] 1.2 Experimental animals

[0144] A total of 120 calves were selected from a naturally infected herd, with 10 calves in each group.

[0145] 2. Methods

[0146] 2.1 Grouping and Dosing

[0147] 120 calves were selected as experimental animals, with 10 calves in each group. Experimental group 1 was a control group without drug administration, which was not administered and was given the same volume of normal saline orally; experimental group 2 was a commercially available ivermectin injection group, which was administered at 0.02 mL / kg. Experimental groups 3 to 12 were respectively the drug groups of Example 1 and Comparative Examples 1-9, which were orally administered at 1 g / 10 kg body weight. Each group was raised separately under the same feeding conditions.

[0148] 2.2 Experiment on the control effect of nematodes and tapeworms in cattle

[0149] 2.2.1 Test methods

[0150] The drug test group was given the drug according to body weight. The formula for calculating body weight is body weight (kg) = [chest circumference (m)2 × soft italic (m) / 10800] × 0.85. After the drug was administered, the cattle were marked one by one, and they were still grazed with the group on the grassland after the drug administration. The cattle in the control group without drug administration were given the same volume of normal saline. On the day of drug administration (0 day, before drug administration) and on the 3rd, 7th, 14th and 21st days after drug administration, the rectum of each group of test cattle was collected for fecal worm egg count using the McMaster counting method to calculate the number of infections. After the drug administration, the feces was soaked and processed to accurately observe the eggs in the feces, and the types of parasites in the feces were observed using the saturated salt water flotation method. In addition, pay attention to whether there are yellow or white cylindrical egg-bearing segments in the feces.

[0151] 2.2.2 Criteria for determining efficacy

[0152] According to the changes in eggs (larvae) in the feces of each group of cattle before and after deworming, observe whether the clinical symptoms (diarrhea, weight loss, changes in fecal characteristics or vomiting, etc.) disappear, the reduction rate of nematodes and eggs in feces is ≥90%, and the physical signs of the test animals return to normal. The main statistics are the egg reduction rate and deworming rate, and the deworming effect is calculated according to the following formula:

[0153] Egg reduction rate (%) = (number of eggs per gram of feces before the test - number of eggs per gram of feces after the test) / number of eggs per gram of feces before the test × 100%;

[0154] Deworming rate (%) = (average infection intensity before the experiment - average infection intensity after the experiment) / average infection intensity before the experiment × 100%;

[0155] Relative anthelmintic rate (%) = (number of worms in the non-medicated group - number of worms in the medicated group) / number of worms in the non-medicated group × 100%

[0156] 3. Results and Analysis

[0157] The results of the nematode and tapeworm expelling effects of Example 1 and Comparative Examples 1-9 are shown in Table 4. Comparison of the results of the ivermectin injection group, Example 1, Comparative Example 1, and Comparative Example 2 groups shows that the nematode and tapeworm expelling effect of Example 1 is the best, and the ivermectin injection group has only the nematode expelling effect but no tapeworm expelling effect. Comparative Example 1 has only the Chinese medicine composition of the present invention, which has the effect of expelling tapeworms, but its nematode expelling effect is poor. Comparative Example 2 is composed of the composition of the present invention and ivermectin, and does not contain the vitamin component of the present invention. It has the effect of expelling nematodes and tapeworms, but the effect is slightly worse than that of Example 1.

[0158] The above results show that the combined use of the Chinese and Western medicine combination of the present invention has the effect of expelling nematodes and tapeworms, and the effect is better than the existing combination.

[0159] Table 4 Results of examination of digestive tract nematode eggs in feces

[0160]

[0161]

[0162] EPG: eggs per gram of feces.

[0163] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese and Western medicine composite composition for animals to fight internal and external parasites, characterized in that: The invention is prepared from 20-35 parts of betel nut extract, 35-45 parts of pumpkin seed extract, 20-35 parts of radix changshanii extract, 20-35 parts of artemisia annua extract, 20-40 parts of stemonae extract, 35-50 parts of astragalus extract, 20-50 parts of vitamin A, 10-40 parts of vitamin E and 0.25-0.8 parts of ivermectin.

2. The Chinese and Western medicine composite composition for animals against internal and external parasites according to claim 1, characterized in that: The invention is prepared from 25-35 parts of betel nut extract, 40-45 parts of pumpkin seed extract, 28-35 parts of changshan extract, 25-30 parts of artemisia annua extract, 30-40 parts of stemona extract, 40-50 parts of astragalus extract, 25-50 parts of vitamin A, 25-40 parts of vitamin E and 0.5-0.8 parts of ivermectin.

3. The Chinese and Western medicine composite composition for animals against internal and external parasites according to claim 2, characterized in that: The invention is prepared from 28-35 parts of betel nut extract, 40-45 parts of pumpkin seed extract, 30-35 parts of radix changshanii extract, 27-30 parts of artemisia annua extract, 35-40 parts of stemonae extract, 40-45 parts of astragalus extract, 30-50 parts of vitamin A, 30-40 parts of vitamin E and 0.5-0.8 parts of ivermectin.

4. The Chinese and Western compound composition for animals against internal and external parasites according to claim 3, characterized in that: The invention is prepared from 35 parts of betel nut extract, 45 parts of pumpkin seed extract, 35 parts of changshan extract, 30 parts of artemisia annua extract, 40 parts of stemona extract, 45 parts of astragalus extract, 50 parts of vitamin A, 40 parts of vitamin E and 0.8 parts of ivermectin.

5. The method for preparing the Chinese and Western compound composition for animals against internal and external parasites according to any one of claims 1 to 4, characterized in that: Prepare according to the following steps: (1) Mix and stir the betel nut extract, pumpkin seed extract, Dichroa febrifuga extract, Artemisia annua extract, Stemona japonica extract, and Astragalus membranaceus extract, and filter the mixture through a 200-mesh sieve after stirring evenly, and set aside; (2) Mix vitamin A, vitamin E and ivermectin evenly and set aside; (3) The mixtures obtained in step (1) and step (2) are mixed uniformly, and soluble starch, surfactant, cosolvent, etc. are added, and then flavoring agents and preservatives are added, and mixed thoroughly to obtain a soluble powder.

6. Use of the Chinese and Western compound composition for preventing and treating internal and external parasites for animals as claimed in any one of claims 1 to 4 in the preparation of drugs for preventing and treating internal and external parasites in animals.

7. The use according to claim 6, characterized in that: The animal is a ruminant.

8. The use according to claim 7, characterized in that: The ruminant is a cattle.

9. The use according to claim 6, characterized in that: The external parasite is a cowhide fly.

10. The use according to claim 6, characterized in that: The internal parasites are nematodes and / or tapeworms.

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