Preparation method and application of melatonin molecularly imprinted membrane assembly

Abstract

The invention discloses a preparation method and application of a melatonin molecularly imprinted membrane component, the preparation method is simple and low in cost, the melatonin molecularly imprinted membrane component with high selectivity and specificity is obtained, high-purity extraction can be carried out on a single molecule of a melatonin monomer, and the melatonin molecularly imprinted membrane component with high selectivity and specificity is obtained. The problems of complex flow, low purity and high input cost of the existing melatonin extraction process are solved.

Description

Technical Field

[0001] The invention relates to the technical field of functional materials, and in particular to a preparation method and application of a melatonin molecular imprinting membrane component. Background Art

[0002] Melatonin is an endogenous hormone secreted by the human pineal gland. It plays a vital role in regulating circadian rhythm, sleep cycle, immune function, anti-oxidation and anti-aging physiological processes. In recent years, as people pay more attention to health and quality of life, the market demand for melatonin as a food supplement and drug ingredient has increased. However, there are a series of problems in the traditional melatonin extraction method, such as low synthesis efficiency, cumbersome purification process, unstable product quality, etc. These problems limit the wide application of melatonin in the fields of medicine and health care products. Chinese invention patent CN106543068A discloses a melatonin separation process, and the extraction process steps include: step 1, pretreatment; step 2, crushing; step 3, leaching; step 4, membrane treatment; step 5, decolorization treatment; step 6, rotary evaporation; step 7, drying. Compared with the traditional filtration process, the quality of the product has been improved, and the product yield and production efficiency have been significantly improved. In the present invention, the concentration and separation process is carried out by reverse osmosis membrane, and the purity of the separation still needs to be further improved.

[0003] The basic principle of molecular imprinting technology (MIT) is to interact the template molecule (molecule to be separated and identified) with a functional monomer with a suitable functional group, and form a macroporous, network-like polymer under the action of a crosslinker and an initiator. The template molecule is removed by solvent elution or hydrolysis under certain conditions, leaving a "memory" hole in the polymer that matches the size, shape and functional group of the template molecule. Such a hole can have a specific affinity with the template molecule to be separated in the mixture, thereby achieving separation, purification, pre-enrichment of the template molecule, etc., and then using chemiluminescence methods such as enzyme-linked immunosorbent assay to achieve rapid and effective detection of the template molecule in the sample. However, there has been no report on the preparation of melatonin molecular imprinting polymer membranes so far. Summary of the invention

[0004] The purpose of the present invention is to provide a preparation method and application of a melatonin molecular imprinting membrane assembly. The prepared melatonin molecular imprinting membrane assembly can extract a single molecule of a melatonin monomer with high purity, thereby solving the problems of complex process, low purity and high investment cost in the current melatonin extraction process.

[0005] In order to achieve the above object, the solution of the present invention is:

[0006] A method for preparing a melatonin molecular imprinted membrane assembly comprises the following steps:

[0007] Step 1, firstly, according to the following mass percentages, 1% to 3% melatonin, 3% to 6% functional monomer, 50% to 60% solvent, 30% to 40% cross-linking agent and 1% to 5% initiator are mixed, reacted at 30 to 80° C. for 2 to 24 hours, and then cooled to 20 to 50° C. to obtain a melatonin composite solution;

[0008] Step 2, using a polyethersulfone flat ultrafiltration membrane as a base membrane, applying the prepared melatonin composite solution on the base membrane, drying at 50-100° C., and finally UV curing and hot air drying to obtain the melatonin composite membrane;

[0009] Step 3: The melatonin composite membrane is then processed into a rolled membrane assembly, and the membrane assembly is assembled in a rolled membrane assembly housing. Finally, ethanol is used as an eluent in a membrane device to wash the melatonin template molecules on the membrane assembly to prepare the melatonin molecular imprinted membrane assembly.

[0010] In step 1, the functional monomer is methacrylic acid, the solvent is ethanol, the cross-linking agent is acrylonitrile, and the initiator is azobisisobutyronitrile.

[0011] In step 3, the membrane device is assembled by assembling the membrane assembly in the shell of the spiral membrane assembly, and then assembling it with a booster pump, an electric control, a bracket and a medicine box.

[0012] An application of a melatonin molecular imprinting membrane component is to apply the melatonin molecular imprinting membrane component to the extraction of melatonin.

[0013] After adopting the above technical scheme, the present invention provides a method for preparing a melatonin molecular imprinting membrane component. The preparation method is simple and low in cost, and a melatonin molecular imprinting membrane component with high selectivity and specificity is obtained. A single molecule of melatonin monomer can be extracted with high purity, thereby solving the problems of complex process, low purity and high investment cost in the current melatonin extraction process. DETAILED DESCRIPTION

[0014] In order to further explain the technical solution of the present invention, the present invention is described in detail below through specific embodiments.

[0015] Example 1

[0016] A method for preparing a melatonin molecular imprinted membrane assembly comprises the following steps:

[0017] Step 1: first, according to the following mass percentages, 3% melatonin, 6% methacrylic acid, 50% ethanol, 36% acrylonitrile and 5% azobisisobutyronitrile were mixed in a reaction kettle, reacted at 80° C. for 3 hours, and then cooled to 45° C. to obtain a melatonin composite solution;

[0018] Step 2, then using the polyethersulfone flat ultrafiltration membrane as the base membrane, sending it into a film scraping machine, scraping the prepared melatonin composite solution onto the base membrane, and then sending it into an oven to dry at 60° C., and finally UV curing and hot air drying to obtain a melatonin composite membrane;

[0019] Step 3, the melatonin composite membrane is then processed into a rolled membrane assembly, and the membrane assembly is assembled in a rolled membrane assembly housing, and then assembled into a membrane device equipped with a booster pump, an electronic control, a bracket, and a medicine box. Finally, ethanol is pumped into the membrane device through the booster pump, and ethanol is used as an eluent in the membrane device to wash the melatonin template molecules on the membrane assembly to prepare a melatonin molecular imprinting membrane assembly.

[0020] Example 2

[0021] A method for preparing a melatonin molecular imprinted membrane assembly comprises the following steps:

[0022] Step 1: first, according to the following mass percentages, 1% melatonin, 4% methacrylic acid, 60% ethanol, 33% acrylonitrile and 2% azobisisobutyronitrile were mixed in a reaction kettle, reacted at 60° C. for 24 hours, and then cooled to 35° C. to obtain a melatonin composite solution;

[0023] Step 2, using the polyethersulfone flat ultrafiltration membrane as the base membrane, sending it into a film scraping machine, scraping the prepared melatonin composite solution onto the base membrane, and then sending it into an oven to dry at 100° C., and finally UV curing and hot air drying to obtain a melatonin composite membrane;

[0024] Step 3, the melatonin composite membrane is then processed into a rolled membrane assembly, and the membrane assembly is assembled in a rolled membrane assembly housing, and then assembled into a membrane device equipped with a booster pump, an electronic control, a bracket, and a medicine box. Finally, ethanol is used as an eluent in the membrane device, and ethanol is pumped into the membrane device through a booster pump to wash the melatonin template molecules on the membrane assembly to prepare a melatonin molecular imprinted membrane assembly.

[0025] The manufacturing process of the wound membrane assembly and the process of assembling the wound membrane assembly into a membrane device involved in the present invention are both well-known technologies in the art.

[0026] Application Examples

[0027] First, the corn is crushed, and then ethanol solvent is added and stirred to obtain a corn solution. The corn solution is then poured into a bag filter for filtration to remove large particle impurities. The obtained filtrate enters a membrane device assembled by melatonin molecular imprinting membrane components for purification to obtain a melatonin extract, and finally powder spraying and drying are performed to obtain high-purity melatonin.

[0028] The above embodiments do not limit the product form and style of the present invention. Any appropriate changes or modifications made by ordinary technicians in the relevant technical field should be deemed to be within the patent scope of the present invention.

Claims

1. A method for preparing a melatonin molecular imprinted membrane assembly, characterized in that: The following steps are involved: Step 1, firstly, according to the following mass percentages, 1% to 3% melatonin, 3% to 6% functional monomer, 50% to 60% solvent, 30% to 40% cross-linking agent and 1% to 5% initiator are mixed, reacted at 30 to 80° C. for 2 to 24 hours, and then cooled to 20 to 50° C. to obtain a melatonin composite solution; Step 2, using a polyethersulfone flat ultrafiltration membrane as a base membrane, applying the prepared melatonin composite solution on the base membrane, drying at 50-100° C., and finally UV curing and hot air drying to obtain the melatonin composite membrane; Step 3: The melatonin composite membrane is then processed into a rolled membrane assembly, and the membrane assembly is assembled in a rolled membrane assembly housing. Finally, ethanol is used as an eluent in a membrane device to wash the melatonin template molecules on the membrane assembly to prepare the melatonin molecular imprinted membrane assembly.

2. The method for preparing a melatonin molecularly imprinted membrane assembly according to claim 1, characterized in that: In step 1, the functional monomer is methacrylic acid, the solvent is ethanol, the cross-linking agent is acrylonitrile, and the initiator is azobisisobutyronitrile.

3. The method for preparing a melatonin molecularly imprinted membrane assembly according to claim 1, characterized in that: In step 3, the membrane device is assembled by assembling the membrane assembly in the shell of the spiral membrane assembly, and then assembling it with a booster pump, an electric control, a bracket and a medicine box.

4. An application of a melatonin molecular imprinted membrane assembly, characterized in that: The melatonin molecular imprinting membrane assembly as claimed in claim 1 is applied to the extraction of melatonin.