Equipment, system and method based on multi-stage fingerprint spectrum

Abstract

The invention discloses equipment, system and method based on a multi-stage fingerprint spectrum, the equipment comprises a pre-pretreatment device, a multi-stage membrane separation device and a fluorescence fingerprint spectrum fluorescence signal catcher, the output end of the pre-pretreatment device is connected to the input end of the multi-stage membrane separation device, and the output end of the multi-stage membrane separation device is connected to the output end of the fluorescence fingerprint spectrum. The output end of the multi-stage membrane separation device is connected to the input end of the fluorescent signal catcher of the fluorescent fingerprint spectrum, the pre-pretreatment device is provided with a sample collection material chamber, and a filter bag is arranged at the upper end of the sample collection material chamber. According to the multi-stage membrane separation device, fingerprint spectrums are collected in different stages of membrane multi-stage separation, information of multiple levels is obtained, different components in a water sample can be identified and analyzed more accurately in combination with the fingerprint spectrums, the analysis accuracy is improved, and meanwhile the detection sensitivity and efficiency are improved.

Description

Technical Field

[0001] The present application relates to the field of fingerprint technology, and in particular to a device, system and method based on multi-level fingerprint. Background Art

[0002] Three-dimensional fluorescence spectroscopy uses three coordinate axes to describe the fluorescence spectrum: excitation wavelength, emission wavelength, and fluorescence intensity. The excitation wavelength refers to the wavelength of the incident light used to excite the sample to emit fluorescence, while the emission wavelength refers to the wavelength range in which the fluorescence emission is recorded. The fluorescence intensity indicates the intensity of the fluorescence signal. By analyzing the changes in the fluorescence intensity of the sample at different excitation and emission wavelengths, information about the relative content of different molecules in the sample, the position and shape of the fluorescence peak, etc. can be obtained.

[0003] In many scientific and engineering applications, comprehensive analysis and characterization of samples are essential to solving various problems. Traditional fluorescence spectroscopy analysis methods usually only focus on the relationship between fluorescence intensity and wavelength. This analysis method has the following limitations:

[0004] Limited data dimensionality: Traditional fluorescence spectroscopy methods usually provide limited data dimensionality and cannot meet the needs of complex samples. In many cases, samples may contain multiple fluorescent dyes, multiple molecules, or complex fluorescence signals.

[0005] Signal interference: The fluorescence signals of different components in a complex mixture often interfere with each other, making it difficult to accurately separate and identify them.

[0006] Low resolution: Traditional methods have limited resolution and cannot provide enough detail to distinguish similar signals or sample components.

[0007] Data loss: Traditional methods often result in partial data loss because they cannot effectively handle the multidimensional characteristics of complex samples.

[0008] Single performance: Traditional methods are usually limited to specific types of samples or molecules and are difficult to adapt to a wide range of analytical needs.

[0009] In view of the above problems, effective technical solutions are urgently needed. Summary of the invention

[0010] The purpose of this application is to provide a device, system and method based on multi-level fingerprints, which improves the accuracy and efficiency of sample analysis and can obtain information at different levels.

[0011] The present application provides a device based on a multi-stage fingerprint spectrum, comprising: a pre-pretreatment device, a multi-stage membrane separation device, and a fluorescent signal capturer of a fluorescent fingerprint spectrum. The output end of the pre-pretreatment device is connected to the input end of the multi-stage membrane separation device, and the output end of the multi-stage membrane separation device is connected to the input end of the fluorescent signal capturer of the fluorescent fingerprint spectrum. The pre-pretreatment device is provided with a sample collection material chamber, and a filter bag is provided at the upper end of the sample collection material chamber.

[0012] In this scheme, multiple diaphragm pumps are provided inside the multi-stage membrane separation device, the inlet of each diaphragm pump is connected to the material collection chamber, and the outlet of each diaphragm pump is connected in sequence to the accumulator, safety valve, membrane inlet pressure gauge, diaphragm assembly, membrane outlet pressure gauge, regulating valve and returns to the material collection chamber.

[0013] In this solution, different components in the multi-stage membrane separation device are connected by PE pipes, and the model of the PE pipe is determined according to the type of material.

[0014] In this solution, the diaphragm assembly includes: a membrane assembly bottom plate, a diaphragm, a partition, a membrane assembly pressure plate and fasteners. The diaphragm is arranged between the membrane assembly bottom plate and the membrane assembly pressure plate, and the membrane assembly bottom plate and the membrane assembly pressure plate are connected by fasteners.

[0015] In this solution, the membrane selects the corresponding aperture according to the actual material requirements to facilitate subsequent distinction.

[0016] In this solution, a hose is provided at the water outlet end of the membrane of the membrane assembly for collecting the filtered products and loading them into corresponding sample collection chambers.

[0017] In this scheme, the fluorescence signal capturer of the fluorescence fingerprint is used to record and analyze the fluorescence signals captured at each membrane level, including wavelength and fluorescence intensity.

[0018] In this solution, the front end of the fluorescent signal capturer is equipped with a matching sample injector, which adds the samples collected in different sample collection chambers into the fluorescent fingerprint detection device at the rear end in sequence.

[0019] The second aspect of the present invention provides a system based on a multi-level fingerprint spectrum, which includes: an early sample processing system, a late data analysis system and a corresponding multi-level analysis spectrum database and several devices based on the multi-level fingerprint spectrum. The analysis system displays the fluorescence signal matrix obtained by the device in the form of a heat map, and the database organizes the multi-level data of the sample into a sample set as a data sample of the sample.

[0020] The third aspect of the present invention provides a method based on a multi-level fingerprint spectrum, which is applied to the system based on a multi-level fingerprint spectrum, and is characterized in that it comprises the following steps:

[0021] S1: According to the actual situation on site, obtain the target water sample and perform pre-treatment, wherein the pre-treatment includes: sample extraction, dyeing or labeling;

[0022] S2: The pre-treated sample passes through a multi-stage membrane separation device to obtain molecules or particles of different sizes and properties;

[0023] S3: On each membrane level, a fluorescent signal capture device is set to enter the fluorescent detection device in turn. The fluorescent signal includes various wavelengths and fluorescence intensities, reflecting the properties of different molecules or particles;

[0024] S4: captured fluorescence signal data, forming a multi-level fingerprint dataset;

[0025] S5: Use visualization tools to analyze and display the obtained multi-level fingerprint dataset.

[0026] As can be seen from the above, the device, system and method based on multi-level fingerprint spectrum disclosed in the present invention include: a pre-pretreatment device, a multi-level membrane separation device, and a fluorescent signal capturer of the fluorescent fingerprint spectrum. The output end of the pre-pretreatment device is connected to the input end of the multi-level membrane separation device, and the output end of the multi-level membrane separation device is connected to the input end of the fluorescent signal capturer of the fluorescent fingerprint spectrum. The pre-pretreatment device is provided with a sample collection material chamber, and a filter bag is provided at the upper end of the sample collection material chamber. The multi-level membrane separation device proposed in the present invention acquires information of multiple levels by collecting fingerprint spectrum at different stages of multi-level membrane separation. In combination with the fingerprint spectrum, different components in the water sample can be more accurately identified and analyzed, the accuracy of the analysis is improved, and the sensitivity and efficiency of the detection are improved.

[0027] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by practicing the embodiments of the present application. The purpose and other advantages of the present application can be realized and obtained by the structures specifically pointed out in the written description, claims, and drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 A schematic diagram of a device based on a multi-level fingerprint map provided in an embodiment of the present application;

[0030] Figure 2 This is a schematic diagram of the structure of the diaphragm assembly provided in an embodiment of the present application.

[0031] Figure 3 A flow chart of a method based on multi-level fingerprint spectrum provided in an embodiment of the present application. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all of the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the application claimed for protection, but merely represents the selected embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work belong to the scope of protection of the present application.

[0033] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings. At the same time, in the description of this application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0034] Please refer to Figure 1 , is a schematic diagram of a device based on a multi-level fingerprint spectrum in some embodiments of the present application. The device based on a multi-level fingerprint spectrum is used for sample analysis equipment, systems, etc. The device based on a multi-level fingerprint spectrum includes: a pre-pretreatment device, a multi-level membrane separation device, and a fluorescent signal capturer of a fluorescent fingerprint spectrum. The output end of the pre-pretreatment device is connected to the input end of the multi-level membrane separation device, and the output end of the multi-level membrane separation device is connected to the input end of the fluorescent signal capturer of the fluorescent fingerprint spectrum. The pre-pretreatment device is provided with a raw material chamber 1, and a filter bag 2 is provided at the upper end of the sample collection material chamber.

[0035] It should be noted that the technical purpose of the present invention is to construct a device based on a multi-stage fingerprint spectrum through a pre-treatment device, a multi-stage membrane separation device, and a fluorescent signal capturer of a fluorescent fingerprint spectrum. By collecting fingerprints at different stages of membrane multi-stage separation, information at multiple levels can be obtained. This can provide a more comprehensive water sample analysis, including a detailed understanding of different molecules, particles or compounds. In addition: Membrane multi-stage separation usually has a highly efficient separation and concentration effect, which helps to concentrate the target components before analysis and improve the sensitivity and efficiency of detection.

[0036] The technology combined with fingerprint spectrum usually has a shorter response time, so the present invention can provide real-time water sample monitoring, which is of great significance for timely identification of water quality problems. In addition, the present invention is suitable for multiple application fields such as water treatment, environmental monitoring, food safety and biomedicine.

[0037] According to an embodiment of the present invention, a plurality of diaphragm pumps 4 are provided inside the multi-stage membrane separation device, the inlet of each diaphragm pump 4 is connected to the material collecting chamber, and the outlet of each diaphragm pump is connected in sequence to a safety valve 5, a membrane inlet pressure gauge 6, a diaphragm assembly 7, a membrane outlet pressure gauge 8, and a regulating valve 9 and returns to the material collecting chamber 3.

[0038] It should be noted that the inlet of each diaphragm pump 4 is connected to a material collecting chamber, and the inlet of the subsequent diaphragm pump is connected to the previous material collecting chamber 3 .

[0039] According to an embodiment of the present invention, different components in the multi-stage membrane separation device are connected through a PE pipe 10, and the model of the PE pipe is determined according to the type of material.

[0040] It should be noted that, in a specific embodiment, different material types, such as different fluidity and viscosity of the material, may result in different diameters of the selected PE pipe.

[0041] like Figure 2 As shown, according to an embodiment of the present invention, the diaphragm assembly 7 includes: a membrane assembly bottom plate 11, a diaphragm 12, a partition 13, a membrane assembly pressure plate 14 and a fastener 15, the diaphragm is arranged between the membrane assembly bottom plate 11 and the membrane assembly pressure plate 14, and the membrane assembly bottom plate 11 and the membrane assembly pressure plate 14 are connected by the fastener 15.

[0042] It should be noted that, in a specific embodiment, the fasteners may be screws and nuts.

[0043] According to an embodiment of the present invention, the diaphragm selects a corresponding aperture according to actual material requirements to facilitate subsequent differentiation.

[0044] According to the embodiment of the present invention, a hose 16 is provided at the water outlet end of the membrane of the membrane assembly for collecting filtered products and loading them into corresponding sample collection chambers 17 .

[0045] According to an embodiment of the present invention, the fluorescence signal capturer 18 of the fluorescence fingerprint is used to record and analyze the fluorescence signals captured at each membrane level, including wavelength and fluorescence intensity.

[0046] According to the embodiment of the present invention, the front end of the fluorescent signal capturer is provided with a matching sample injector 19, which sequentially adds the samples collected in different sample collection chambers into the fluorescent fingerprint detection device at the rear end.

[0047] It should be noted that the sample added to the fluorescence signal capturer of the fluorescence fingerprint spectrum requires rinsing the quartz dish 20 of the detection equipment.

[0048] The second aspect of the present invention provides a system based on a multi-level fingerprint spectrum, which includes: an early sample processing system, a late data analysis system and a corresponding multi-level analysis spectrum database and several devices based on the multi-level fingerprint spectrum. The analysis system displays the fluorescence signal matrix obtained by the device in the form of a heat map, and the database organizes the multi-level data of the sample into a sample set as a data sample of the sample.

[0049] like Figure 3 As shown, the third aspect of the present invention provides a method based on a multi-level fingerprint spectrum, and the method is applied to the system based on a multi-level fingerprint spectrum, and is characterized in that it includes the following steps:

[0050] S1: According to the actual situation on site, obtain the target water sample and perform pre-treatment, wherein the pre-treatment includes: sample extraction, dyeing or labeling;

[0051] S2: The pre-treated sample passes through a multi-stage membrane separation device to obtain molecules or particles of different sizes and properties; the device includes a series of membrane filters of different levels, each filter has a specific pore size. The sample is pushed through these membrane filters, where molecules or particles of different sizes and properties are separated and pass through their respective membrane levels.

[0052] S3: On each membrane level, a fluorescent signal capture device is set to enter the fluorescent detection device in turn. The fluorescent signal includes various wavelengths and fluorescence intensities, reflecting the properties of different molecules or particles;

[0053] S4: The captured fluorescence signal data forms a multi-level fingerprint dataset; This dataset contains multi-dimensional information of fluorescence signals at different membrane levels.

[0054] S5: Use visualization tools to analyze and display the obtained multi-level fingerprint dataset. This enables users to understand the characteristics of multi-level samples more comprehensively and intuitively.

[0055] It should be noted that the pretreated samples are separated using a multi-stage membrane separation device, in which each membrane level has a specific pore size, which is used to effectively separate molecules or particles of different sizes and properties. The samples at different levels are added in turn to the fluorescent signal capturer of the fluorescent fingerprint to obtain multi-level data of the samples, and the fluorescent signals captured at each membrane level, including wavelength and fluorescence intensity, are recorded and analyzed. The captured data are organized into a three-dimensional data set and displayed through visualization tools.

[0056] In a specific embodiment, the domestic sewage in a certain city sewage pipe network is taken as an example to illustrate the present invention. According to the actual situation on site, the target water sample is obtained, and the necessary pre-treatment steps are performed, and preliminary filtration is performed to ensure that there is no suspended matter affecting the detection result. The treated sample is passed through a multi-stage membrane separation device, and the sample is pushed through these membrane filters, where molecules or particles of different sizes and properties are separated and pass through their respective membrane levels. The obtained multi-level samples enter the fluorescence detection device in turn, and the captured fluorescence signal data is collected and sorted by the data integration module to form a multi-level fingerprint data set.

[0057] In another embodiment, the tail water of a certain city sewage treatment plant is taken, and the target water sample is obtained according to the actual situation on site, and the necessary pre-treatment steps are carried out, and preliminary filtration is performed to ensure that there is no suspended matter affecting the detection results. The treated sample is passed through a multi-stage membrane separation device, and the sample is pushed through these membrane filters, where molecules or particles of different sizes and properties are separated and pass through their respective membrane levels. The obtained multi-level samples enter the fluorescence detection device in turn, and the captured fluorescence signal data is collected and sorted by the data integration module to form a multi-level fingerprint data set.

[0058] In a specific embodiment, the tail water of a farm is taken, and the target water sample is obtained according to the actual situation on site, and the necessary pre-treatment steps are performed, and preliminary filtration is performed to ensure that there is no suspended matter affecting the test results. The treated sample is passed through a multi-stage membrane separation device, and the sample is pushed through these membrane filters, where molecules or particles of different sizes and properties are separated and pass through their respective membrane levels. The obtained multi-level samples enter the fluorescence detection device in turn, and the captured fluorescence signal data is collected and sorted by the data integration module to form a multi-level fingerprint data set.

[0059] The present invention discloses a device, system and method based on a multi-level fingerprint spectrum, the device includes: a pre-pretreatment device, a multi-level membrane separation device, and a fluorescent signal capturer of a fluorescent fingerprint spectrum, the output end of the pre-pretreatment device is connected to the input end of the multi-level membrane separation device, the output end of the multi-level membrane separation device is connected to the input end of the fluorescent signal capturer of the fluorescent fingerprint spectrum, the pre-pretreatment device is provided with a sample collection material chamber, and a filter bag is provided at the upper end of the sample collection material chamber. The multi-level membrane separation device proposed by the present invention acquires information of multiple levels by collecting fingerprints at different stages of multi-level membrane separation, and can more accurately identify and analyze different components in a water sample in combination with the fingerprint spectrum, thereby improving the accuracy of the analysis and the sensitivity and efficiency of the detection.

[0060] In the several embodiments provided in the present application, it should be understood that the disclosed devices and methods can be implemented in other ways. The device embodiments described above are only schematic. For example, the division of the units is only a logical function division. There may be other division methods in actual implementation, such as: multiple units or components can be combined, or can be integrated into another system, or some features can be ignored or not executed. In addition, the coupling, direct coupling, or communication connection between the components shown or discussed can be through some interfaces, and the indirect coupling or communication connection of the devices or units can be electrical, mechanical or other forms.

[0061] The units described above as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units; they may be located in one place or distributed on multiple network units; some or all of the units may be selected according to actual needs to achieve the purpose of the present embodiment.

[0062] In addition, all functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may be separately used as a unit, or two or more units may be integrated into one unit; the above-mentioned integrated units may be implemented in the form of hardware or in the form of hardware plus software functional units.

[0063] Those skilled in the art can understand that: all or part of the steps of implementing the above method embodiments can be completed by hardware related to program instructions, and the aforementioned program can be stored in a readable storage medium. When the program is executed, it executes the steps of the above method embodiments; and the aforementioned storage medium includes: mobile storage devices, read-only memories (ROM, Read-Only Memory), random access memories (RAM, Random Access Memory), disks or optical disks, and other media that can store program codes.

[0064] Alternatively, if the above-mentioned integrated unit of the present invention is implemented in the form of a software function module and sold or used as an independent product, it can also be stored in a readable storage medium. Based on this understanding, the technical solution of the embodiment of the present invention can be essentially or partly reflected in the form of a software product that contributes to the prior art. The software product is stored in a storage medium and includes several instructions for a computer device (which can be a personal computer, a server, or a network device, etc.) to execute all or part of the methods described in each embodiment of the present invention. The aforementioned storage medium includes: various media that can store program codes, such as mobile storage devices, ROM, RAM, magnetic disks or optical disks.

Claims

1. A device based on multi-level fingerprint, characterized in that: include: A pre-treatment device, a multi-stage membrane separation device, and a fluorescent signal capturer of a fluorescent fingerprint spectrum. The output end of the pre-treatment device is connected to the input end of the multi-stage membrane separation device, and the output end of the multi-stage membrane separation device is connected to the input end of the fluorescent signal capturer of the fluorescent fingerprint spectrum. The pre-treatment device is provided with a sample collection material chamber, and a filter bag is provided at the upper end of the sample collection material chamber.

2. The device based on multi-level fingerprint spectrum according to claim 1, characterized in that: The multi-stage membrane separation device is internally provided with a plurality of diaphragm pumps, the inlet of each diaphragm pump is connected to the material collection chamber, and the outlet of each diaphragm pump is sequentially connected to the accumulator, the safety valve, the membrane inlet pressure gauge, the diaphragm assembly, the membrane outlet pressure gauge, the regulating valve and returns to the material collection chamber.

3. The device based on multi-level fingerprint spectrum according to claim 2, characterized in that: Different components in the multi-stage membrane separation device are connected by PE pipes, and the model of the PE pipe is determined according to the type of material.

4. The device based on multi-level fingerprint spectrum according to claim 2, characterized in that: The diaphragm assembly comprises: a membrane assembly bottom plate, a diaphragm, a partition, a membrane assembly pressure plate and fasteners. The diaphragm is arranged between the membrane assembly bottom plate and the membrane assembly pressure plate, and the membrane assembly bottom plate and the membrane assembly pressure plate are connected by fasteners.

5. The device based on multi-level fingerprint spectrum according to claim 4, characterized in that: The membrane selects the corresponding aperture according to the actual material requirements to facilitate subsequent distinction.

6. The device based on multi-level fingerprint spectrum according to claim 2, characterized in that: A hose is provided at the water outlet end of the membrane of the membrane assembly for collecting filtered products and loading them into corresponding sample collection chambers.

7. The device based on multi-level fingerprint spectrum according to claim 1, characterized in that: The fluorescence signal capturer of the fluorescence fingerprint is used to record and analyze the fluorescence signals captured at each membrane level, including wavelength and fluorescence intensity.

8. The device based on multi-level fingerprint spectrum according to claim 6, characterized in that: The front end of the fluorescent signal capturer is provided with a matching sample injector, which sequentially adds the samples collected in different sample collection chambers into the fluorescent fingerprint spectrum detection device at the rear end.

9. A system based on multi-level fingerprint spectrum, characterized in that: The system based on multi-level fingerprint spectrum includes: an early sample processing system, a late data analysis system and a corresponding multi-level analysis spectrum database and a plurality of multi-level fingerprint spectrum-based devices as described in any one of claims 1 to 8. The analysis system displays the fluorescence signal matrix obtained by the device in the form of a heat map, and the database organizes the multi-level data of the sample into a sample set as a data sample of the sample.

10. A method based on multi-level fingerprint spectrum, the method is applied to the system based on multi-level fingerprint spectrum according to claim 9, characterized in that: The following steps are involved: S1: According to the actual situation on site, obtain the target water sample and perform pre-treatment, wherein the pre-treatment includes: sample extraction, dyeing or labeling; S2: The pre-treated sample passes through a multi-stage membrane separation device to obtain molecules or particles of different sizes and properties; S3: On each membrane level, a fluorescent signal capture device is set to enter the fluorescent detection device in turn. The fluorescent signal includes various wavelengths and fluorescence intensities, reflecting the properties of different molecules or particles; S4: forming a multi-level fingerprint dataset from the captured fluorescence signal data; S5: Use visualization tools to analyze and display the obtained multi-level fingerprint dataset.

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