Anti-pollution blood gas detection reagent card and preparation method thereof

Abstract

The invention discloses an anti-pollution blood gas detection reagent card and a preparation method thereof, and the preparation method comprises the following steps: placing a blood gas detection reagent card plastic part in a mixed solution of a compound containing an o-phenol structure and a silver ion compound for reaction, cleaning and drying, and dispensing a sensor on the surface of the blood gas detection reagent card plastic part; the compound containing the o-phenol structure is tannic acid, gallic acid, dopamine or catecholamine. When the blood gas detection reagent card is in contact with blood, silver ions on the blood gas detection reagent card can catalyze release of nitric oxide, inhibit platelet adsorption and activation, prevent thrombus clots from being formed on the surfaces of a flow channel and a sensor, improve blood compatibility and ensure that the sensitivity of the sensor on the reagent card is stable within 30 days; the stability and the accuracy of blood gas detection are improved; the anti-pollution blood gas detection reagent card has long-acting anti-pollution capacity, the workload of operators is reduced, and the maintenance cost is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomaterials, and more specifically, to an anti-contamination blood gas detection reagent card and a preparation method thereof. Background Art

[0002] Blood gas analyzers are often used to diagnose clinical symptoms of critically ill patients such as respiratory failure and metabolic imbalance. Common blood gas analyzers are divided into two categories: dry and wet types according to the conditions of use of the test reagent card. The test reagent card that comes with the dry blood gas analyzer can only be used once and is thrown away after use, so there is no problem of contamination. After installation, the test reagent card that comes with the wet blood gas analyzer has a validity period of up to 30 days, during which a large number of sample tests will be performed. Due to long-term contact with blood samples from different sources, the sensor on the surface of the reagent card is prone to platelet aggregation and fibrin accumulation, leading to the formation of thrombus and clots, which in turn hinders the contact between the sensor and the blood sample, affecting the accuracy of the measurement results, and more seriously may cause blockage of the internal flow channel of the reagent card. Regularly removing the reagent card and cleaning the surface of the sensor or adding a cleaning solution containing a decontamination component for flushing is a common method to solve the problem of sensor contamination by blood at this stage, but it requires the operator to manually handle it or run the internal cleaning program of the analyzer.

[0003] Nitric oxide is an important cell signaling factor in the human body, which can inhibit the aggregation and activation of platelets, reduce the formation of thrombus, and anti-inflammatory reaction. The blood contains a series of nitric oxide donors S-nitrosothiol, which can release nitric oxide under the catalytic action of metal ions. In recent years, studies have proposed to construct a catalytic system on the surface of biomaterials, such as the invention patent CN104194460B, which mixes soluble copper salts with compounds having an ortho-phenol structure to obtain a complex of copper ions and polyphenol ligands with nitric oxide catalytic function. Invention patent CN113599581B uses silk fibroin and chitosan systems to fix copper ions and catalyze the release of nitric oxide. The above studies show that the biocompatibility of biomaterials can be greatly improved by treating the surface of biomaterials. Inspired by this, if a modified coating can be prepared on the surface of a blood gas detection reagent card to catalyze the release of nitric oxide from blood, it is also possible to reduce the attachment and deposition of blood substances such as blood cells and proteins, thereby helping to improve the anti-pollution ability of the reagent card. Summary of the invention

[0004] Based on this, the purpose of the present invention is to provide an anti-pollution blood gas detection reagent card and a preparation method thereof, which can significantly reduce the adhesion and deposition of blood substances such as blood cells and proteins on the sensor of the reagent card and has strong anti-pollution ability.

[0005] The specific technical solutions for achieving the above-mentioned invention objectives include the following.

[0006] The first aspect of the present invention provides a method for preparing an anti-pollution blood gas detection reagent card, comprising the following steps: placing a blood gas detection reagent card plastic part in a mixed solution of 0.4 mg / mL to 2.0 mg / mL of a compound containing an ortho-phenol structure and 4 mg / mL to 10 mg / mL of a silver ion compound for reaction, washing and drying, and then spot-coating a sensor on the surface of the blood gas detection reagent card plastic part; the compound containing an ortho-phenol structure is tannic acid, gallic acid, dopamine or catecholamine.

[0007] The second aspect of the present invention provides an anti-contamination blood gas detection reagent card prepared by the above preparation method.

[0008] Aiming at the problem that a blood gas detection reagent card matched with a wet blood gas analyzer is contaminated due to the attachment of blood substances, resulting in decreased sensitivity of a sensor on the reagent card and inaccurate measurement, the present invention proposes a method for preparing an anti-contamination blood gas detection reagent card, wherein a plastic part of the blood gas detection reagent card is immersed in a mixed solution of a certain concentration of a compound containing an o-phenol structure (such as tannic acid, gallic acid, dopamine, catecholamine, etc.) and silver ions (such as silver chloride, etc.), and an appropriate amount of silver ions are stably fixed on the surface of a flow channel of the plastic part of the blood gas detection reagent card by means of the coordination binding effect between the compound containing an o-phenol structure and the silver ions. When the silver ions on the blood gas detection reagent card come into contact with blood, they can catalyze the release of nitric oxide with a moderate release efficiency, inhibit platelet adsorption and activation, avoid the formation of thrombus clots on the flow channel and the sensor surface, improve blood compatibility, ensure that within 30 days, the sensitivity of the sensor on the reagent card is stable, and the stability and accuracy of blood gas detection are improved.

[0009] The present invention can prepare the anti-contamination blood gas detection reagent card through a one-step soaking method, the operation is simple and controllable, the reaction conditions are mild, no complicated and expensive equipment is required, and the preparation cost is low.

[0010] The anti-pollution blood gas detection reagent card of the present invention has a long-term anti-pollution capability and does not require additional cleaning during the effective period of use, thereby reducing the workload of operators and lowering maintenance costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 These are the results of the number of platelets adhered to the surface of the blood gas detection reagent cards of Examples 1 to 4 and Comparative Examples 1 to 4 in Test Example 1 of the present invention.

[0012] Figure 2 In Experimental Example 2 of the present invention, the relative deviation of blood samples tested using the blood gas detection reagent card of Example 4 and a commercial reagent card is shown.

[0013] Figure 3This is the relative deviation of blood samples tested using the blood gas detection reagent card of Comparative Example 1 and the commercial reagent card in Experimental Example 2 of the present invention.

[0014] Figure 4 This is the relative deviation of blood samples tested using the blood gas detection reagent card of Comparative Example 3 and the commercial reagent card in Experimental Example 2 of the present invention.

[0015] Figure 5 This is the relative deviation of blood samples tested using the blood gas detection reagent card of Comparative Example 4 and the commercial reagent card in Experimental Example 2 of the present invention. DETAILED DESCRIPTION

[0016] In order to facilitate the understanding of the present invention, the present invention will be described more fully below. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0017] Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used in the present invention includes any and all combinations of one or more of the related listed items.

[0018] Unless otherwise specified, the examples are all based on conventional experimental conditions or the conditions recommended by the manufacturer's instructions. The raw materials and reagents used in the present invention are all commercially available, and any biological germplasm material can be provided to the outside for scientific research.

[0019] In some embodiments of the present invention, a method for preparing an anti-pollution blood gas detection reagent card is disclosed, comprising the following steps: placing a blood gas detection reagent card plastic part in a mixed solution of 0.4 mg / mL to 2.0 mg / mL of a compound containing an ortho-phenol structure and 4 mg / mL to 10 mg / mL of a silver ion compound for reaction, washing and drying, and then spot-coating a sensor on the surface of the blood gas detection reagent card plastic part; the compound containing an ortho-phenol structure is tannic acid, gallic acid, dopamine or catecholamine.

[0020] In some embodiments, the concentration of the silver ion compound in the mixed solution is 6 mg / mL to 10 mg / mL.

[0021] In some embodiments, the concentration of the silver ion compound in the mixed solution is 8 mg / mL to 10 mg / mL.

[0022] In some embodiments, the compound containing an o-phenol structure in the mixed solution is tannic acid, the silver ion compound is silver chloride, the concentration of the tannic acid is 0.8 mg / mL to 2.0 mg / mL, and the concentration of the silver chloride is 8 mg / mL to 10 mg / mL.

[0023] In some embodiments, the pH of the mixed solution is 7-9.

[0024] In some embodiments, the pH of the mixed solution is 7-7.5.

[0025] In some embodiments, the reaction temperature is 15° C. to 45° C., and the reaction time is 10 h to 30 h.

[0026] In some embodiments, the reaction temperature is 25° C. to 37° C., and the reaction time is 12 h to 24 h.

[0027] In some embodiments, the sensor is a potassium ion sensor.

[0028] In some of the embodiments, a method for preparing an anti-contamination blood gas detection reagent card comprises the following steps: placing a plastic part of the blood gas detection reagent card in a mixed solution containing 0.8 mg / mL to 2.0 mg / mL of tannic acid and 8 mg / mL to 10 mg / mL of silver chloride with a pH of 7 to 7.5, reacting at 25°C to 37°C for 12h to 24h, washing with ultrapure water, drying, and then spot-coating a potassium ion sensor on the surface of the plastic part of the blood gas detection reagent card.

[0029] In other embodiments of the present invention, a contamination-resistant blood gas detection reagent card prepared by the above-mentioned preparation method is disclosed.

[0030] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Example 1 Preparation method of anti-contamination blood gas detection reagent card

[0032] This embodiment provides a method for preparing an anti-contamination blood gas detection reagent card, comprising the following steps:

[0033] 1. According to conventional methods, cleaned and dried polycarbonate is used as the base material, i.e., the blood gas test reagent card plastic part;

[0034] 2. Place the above blood gas test reagent card plastic into a mixed solution (pH 7.5) containing 0.4 mg / mL tannic acid and 4 mg / mL silver chloride, and react at 37°C for 12 hours;

[0035] 3. Use ultrapure water to clean the plastic surface of the blood gas test reagent card 1 to 2 times and dry it at 37°C;

[0036] 4. Apply potassium ion sensor solution (conventional potassium ion sensor solution in the art) to the surface of the plastic part of the blood gas detection reagent card to obtain an anti-pollution blood gas detection reagent card. Example 2 Preparation method of anti-pollution blood gas detection reagent card

[0037] This embodiment provides a method for preparing an anti-contamination blood gas detection reagent card, comprising the following steps:

[0038] 1. According to conventional methods, cleaned and dried polycarbonate is used as the base material, i.e., the blood gas test reagent card plastic part;

[0039] 2. Place the above blood gas test reagent card plastic into a mixed solution (pH 7.5) containing 0.4 mg / mL tannic acid and 6 mg / mL silver chloride, and react at 25°C for 24 hours;

[0040] 3. Use ultrapure water to clean the plastic surface of the blood gas test reagent card 1 to 2 times and dry it at 37°C;

[0041] 4. By applying potassium ion sensor solution, the potassium ion sensor is applied on the plastic surface of the blood gas test reagent card to obtain an anti-contamination blood gas test reagent card.

[0042] Example 3 Preparation method of anti-contamination blood gas detection reagent card

[0043] This embodiment provides a method for preparing an anti-contamination blood gas detection reagent card, comprising the following steps:

[0044] 1. According to conventional methods, cleaned and dried polycarbonate is used as the base material, i.e., the blood gas test reagent card plastic part;

[0045] 2. Place the above blood gas test reagent card plastic into a mixed solution (pH 7.5) containing 0.4 mg / mL tannic acid and 8 mg / mL silver chloride, and react at 37°C for 12 hours;

[0046] 3. Use ultrapure water to clean the plastic surface of the blood gas test reagent card 1 to 2 times and dry it at 37°C;

[0047] 4. By applying potassium ion sensor solution, the potassium ion sensor is applied on the plastic surface of the blood gas test reagent card to obtain an anti-contamination blood gas test reagent card.

[0048] Example 4 Preparation method of anti-contamination blood gas detection reagent card

[0049] This embodiment provides a method for preparing an anti-contamination blood gas detection reagent card, comprising the following steps:

[0050] 1. According to conventional methods, a blood gas test reagent card plastic part is prepared using the cleaned and dried polycarbonate as the base material;

[0051] 2. Place the above blood gas test reagent card plastic into a mixed solution (pH 7.5) containing 0.4 mg / mL tannic acid and 10 mg / mL silver chloride, and react at 25°C for 24 hours;

[0052] 3. Use ultrapure water to clean the plastic surface of the blood gas test reagent card 1 to 2 times and dry it at 37°C;

[0053] 4. By applying potassium ion sensor solution, the potassium ion sensor is applied on the plastic surface of the blood gas test reagent card to obtain an anti-contamination blood gas test reagent card.

[0054] Example 5 Preparation method of anti-contamination blood gas detection reagent card

[0055] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 0.8 mg / mL of tannic acid and 4 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0056] Example 6 Preparation method of anti-contamination blood gas detection reagent card

[0057] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 1.2 mg / mL of tannic acid and 4 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0058] Example 7 Preparation method of anti-contamination blood gas detection reagent card

[0059] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 1.6 mg / mL of tannic acid and 4 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0060] Example 8 Preparation method of anti-contamination blood gas detection reagent card

[0061] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 2.0 mg / mL of tannic acid and 4 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0062] Example 9 Preparation method of anti-contamination blood gas detection reagent card

[0063] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 0.5 mg / mL of gallic acid and 8 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0064] Example 10 Preparation method of anti-contamination blood gas detection reagent card

[0065] This embodiment provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 1.2 mg / mL of gallic acid and 7.5 mg / mL of silver chloride, the other steps are the same as those in embodiment 1.

[0066] Comparative Example 1

[0067] This comparative example provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains only 0.4 mg / mL of tannic acid, the other steps are the same as those in Example 1.

[0068] Comparative Example 2

[0069] This comparative example provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains only 4 mg / mL of silver chloride, the other steps are the same as those in Example 1.

[0070] Comparative Example 3

[0071] This comparative example provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 0.4 mg / mL of tannic acid and 2 mg / mL of silver chloride, the other steps are the same as those in Example 1.

[0072] Comparative Example 4

[0073] This comparative example provides a method for preparing a blood gas detection reagent card. Except that the mixed solution in step 2 contains 1.2 mg / mL of tannic acid and 12 mg / mL of silver chloride, the other steps are the same as those in Example 1.

[0074] Test Example 1 Performance Test of Blood Gas Detection Reagent Card

[0075] The following performance tests were conducted on the blood gas detection reagent cards prepared in Examples 1 to 8 and Comparative Examples 1 to 4.

[0076] 1. Catalytic rate of nitric oxide

[0077] The release rate of catalytic nitric oxide was tested on the blood gas detection reagent cards in Examples 1 to 8 and Comparative Examples 1 to 4 using a NO analyzer. The results are shown in Table 1.

[0078] Table 1

[0079] Test subjects <![CDATA[NO release rate / 10 -10 mol·cm -1 ·min -1 > Example 1 3.1 Example 2 5.8 Example 3 8.2 Example 4 10.3 Example 5 3.2 Example 6 3.4 Example 7 3.59 Example 8 3.7 Comparative Example 1 0.1 Comparative Example 2 0.12 Comparative Example 3 1.5 Comparative Example 4 19.7

[0080] It can be seen from the results in Table 1 that as the concentration of silver ions added to the mixed solution increases, the rate of catalyzing the release of nitric oxide continues to increase (Examples 1 to 4). This is because the amount of silver ions chelated and fixed on the plastic parts of the blood gas detection reagent card increases. In the blood gas detection reagent cards of Examples 5 to 8, the concentration of silver ions in the mixed solution remains unchanged during the preparation process (the same as in Example 1), and the rate of catalyzing the release of nitric oxide is close to that of the blood gas detection reagent card of Example 1, and does not increase with the increase of the content of tannic acid in the mixed solution. In Comparative Example 3, the concentration of silver ions added to the mixed solution is very low, and its blood gas detection reagent card detects a small amount (1.5 / 10 -10 mol cm -1 ·min -1 ) of nitric oxide release, the silver ion concentration added to the mixed solution in Comparative Example 4 was very high, and its blood gas test reagent card detected a large amount (19.7 / 10 -10 mol cm -1 ·min -1 ) of nitric oxide release. Therefore, the concentration of silver ions in the mixed solution plays a key role in catalyzing the release rate of nitric oxide. The higher the silver ion concentration, the more silver ions are chelated and fixed on the surface of the reagent card, and the greater the rate of catalyzing the release of nitric oxide.

[0081] The blood gas detection reagent card of Comparative Example 1 was prepared using only tannic acid solution, and the blood gas detection reagent card of Comparative Example 2 was prepared using only silver chloride solution. No release of nitric oxide was detected in either of them. This is because tannic acid can form coordination bonds with silver ions through the phenolic hydroxyl groups in its own structure, thereby fixing silver ions on the surface of the plastic part, thereby catalyzing the release of nitric oxide. Using only a single component of tannic acid or silver chloride to treat the surface of the reagent card plastic part cannot achieve the purpose of catalyzing the release of nitric oxide.

[0082] 2. Number of Adherent Platelets

[0083] The blood gas detection reagent cards prepared in Examples 1 to 4 and Comparative Examples 1 to 4 were placed in blood (samples were from volunteers) and allowed to stand for 120 minutes, and the number of platelets adhered to the surface of the blood gas detection reagent cards was compared (the blood on the surface of the reagent cards was first rinsed with physiological saline, and the reagent cards were soaked in 2.5% glutaraldehyde for 12 hours to fix the platelets on the surface; then the reagent cards were soaked in 50%, 75%, 90%, and 100% ethanol solutions in sequence to dehydrate the platelets; then the reagent cards were sprayed with gold, and the platelets were observed and counted using a scanning electron microscope). The results are as follows: Figure 1 shown.

[0084] from Figure 1It can be seen that as the rate of catalyzing the release of nitric oxide on the reagent card surface increases, the number of platelets adhering to the reagent card surface continues to decrease (Comparative Example 4 < Example 4 < Example 3 < Example 2 < Example 1 < Comparative Example 3). In Comparative Examples 1 and 2, since the release of nitric oxide cannot be catalyzed, the number of platelets on the reagent card surface is significantly greater. Therefore, as the release rate of nitric oxide increases, the inhibitory effect of the reagent card surface on platelet adhesion is also improved, so that the reagent card has better anti-pollution ability.

[0085] 3. Sensitivity of potassium ion sensor of reagent card

[0086] The blood gas detection reagent cards prepared in Examples 1 to 4 and Comparative Examples 1 to 4 were immersed in blood for 30 days, 120 minutes per day, and the sensitivity of the potassium ion sensor on the reagent card was tested every 5 days during this period (unit: mv / dec); the sensitivity test steps are as follows:

[0087] (1) Prepare two solutions A and B with different potassium ion concentrations: A: potassium ion concentration 7.0 mmmol / L, B: potassium ion concentration 4.5 mmol / L;

[0088] (2) The potassium ion sensor was immersed in the two solutions in turn, and the corresponding potential was measured using an electrochemical workstation. The sensitivity was calculated according to the formula = (potential of solution A - potential of solution B) / lg (concentration of solution A) -lg (concentration of solution B) (lg is the logarithm with base 10).

[0089] The results are shown in Table 2.

[0090] Table 2

[0091] Time / Day Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 0 59.3 57.9 56.7 59 57.6 57.5 57.5 58.7 5 57.6 59 57.8 58.9 51.5 54.6 55.8 65.3 10 59.6 56.2 57 55.3 48.9 52.3 53.2 57.3 15 57 59.1 59.7 58.6 50.3 55.4 53.4 54.8 20 58 56.8 57.3 56.5 45.2 49.3 54.3 58.2 25 58.2 58.1 58.6 58.8 43.3 46.1 52.1 52.3 30 56.5 56.8 58.1 59.1 46.7 44.2 50.2 63.4 AVER 58.0 57.7 57.9 58.0 49.1 48.6 53.8 57.1 SD 1.1 1.1 1.0 1.5 4.7 5.0 2.4 4.5 CV 2.0％ 2.0％ 1.8％ 2.6％ 9.6％ 10.2％ 4.4％ 8.0％

[0092] As can be seen from the results of Table 2, compared with Comparative Examples 1 to 4, the sensitivity of the potassium ion sensor of the blood gas detection reagent card prepared by Examples 1 to 4 is always close to the theoretical Nernst response (59mV / dec), with better stability and smaller coefficient of variation. The blood gas detection reagent cards of Comparative Examples 1 and 2 cannot catalyze the release of nitric oxide, and the platelets accumulated on the surface gradually increase, causing the sensitivity of the potassium ion sensor to decay, with the largest fluctuation range (9.6% and 10.2% respectively). The blood gas detection reagent card of Comparative Example 3 is insufficient due to the release of catalyzed nitric oxide, resulting in the platelet deposition gradually appearing on the sensor surface, and the sensitivity fluctuation range of the potassium ion sensor is also larger than that of Examples 1 to 4 (4.4%). The sensitivity fluctuation of the potassium ion sensor of Comparative Example 4 is also large, and it is always unable to stabilize, because the release of catalyzed nitric oxide is too much, and the gas molecules cannot be quickly dissolved, and are easily aggregated into small bubbles, blocking the potassium ion sensor from contacting the sample solution, affecting the transmission of the electrical signal between the two.

[0093] Therefore, in order to ensure the stability of the sensitivity of the potassium ion sensor on the reagent card, the rate of catalytic nitric oxide release needs to be controlled within a moderate range. When preparing the blood gas detection reagent card, the concentration of silver ions in the mixed solution is maintained at 4 mg / mL to 10 mg / mL, which can ensure that the number of platelets adhering to the surface of the reagent card is low, while also ensuring that the potassium ion sensor has high sensitivity and stability.

[0094] Test Example 2 Testing of blood gas test reagent card on actual samples

[0095] The blood gas detection reagent cards of Example 4 and Comparative Examples 1, 3, and 4 were used to test blood samples (donated by volunteers), and the test was conducted every 5 days, with 10 samples (fresh blood samples) tested each time. Each test result was compared with the reference value of the commercial potassium ion sensor (instrument model: ABL800, manufactured by Radiometer. The instrument is equipped with a flushing solution containing cleaning ingredients, and the cleaning program can be run to prevent contamination, and it can be reused) and the relative deviation (Bc) between the two was calculated. The results are as follows: Figure 2 to Figure 5 shown.

[0096] From the results, it can be seen that the relative deviation of the results can be maintained within the range of ±3% when the blood gas detection reagent card of Example 4 is used for detection, and the detection accuracy is good ( Figure 2 ). The blood gas detection reagent card of comparative example 1 was used for detection. Since the surface of the reagent card could not catalyze the release of nitric oxide, the platelets accumulated on the surface of the potassium ion sensor continued to increase, and the sensor was contaminated by blood. The relative deviation of the measured value on the 5th day exceeded ±3%, and the subsequent deviation range gradually increased, and the test stability and accuracy were significantly deteriorated ( Figure 3 ). The blood gas test reagent card of comparative example 3 was used for testing. Since the surface of the reagent card can only catalyze the release of a small amount of nitric oxide, it cannot maintain the effect of inhibiting platelet adhesion for a long time. After 15 days of testing, the deviation between the measured value and the reference value began to exceed ±3% ( Figure 4 ). When the blood gas test reagent card of comparative example 4 was used for testing, due to the excessive nitric oxide release catalyzed by the surface of the reagent card, the deviation between the measured value and the reference value was always greater than ±3% during the entire test period, and the accuracy of the test could not be guaranteed ( Figure 5 ).

[0097] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0098] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for preparing a contamination-resistant blood gas detection reagent card, characterized in that: The following steps are involved: The blood gas detection reagent card plastic part is placed in a mixed solution containing 0.4 mg / mL to 2.0 mg / mL of a compound containing an ortho-phenol structure and 4 mg / mL to 10 mg / mL of silver ions for reaction, and after cleaning and drying, a sensor is then spot-coated on the surface of the blood gas detection reagent card plastic part; the compound containing an ortho-phenol structure is tannic acid, gallic acid, dopamine or catecholamine.

2. The method for preparing the anti-contamination blood gas detection reagent card according to claim 1, characterized in that: The concentration of silver ions in the mixed solution is 6 mg / mL to 10 mg / mL.

3. The method for preparing the anti-contamination blood gas detection reagent card according to claim 1, characterized in that: The concentration of silver ions in the mixed solution is 8 mg / mL to 10 mg / mL.

4. The method for preparing the anti-contamination blood gas detection reagent card according to claim 1, characterized in that: The compound containing ortho-phenol structure in the mixed solution is tannic acid, the silver ion compound is silver chloride, the concentration of the tannic acid is 0.8 mg / mL to 2.0 mg / mL, and the concentration of the silver chloride is 8 mg / mL to 10 mg / mL.

5. The method for preparing the anti-contamination blood gas detection reagent card according to any one of claims 1 to 4, characterized in that: The pH of the mixed solution is 7 to 9, preferably 7 to 7.

5.

6. The method for preparing the anti-contamination blood gas detection reagent card according to any one of claims 1 to 4, characterized in that: The reaction temperature is 15°C to 45°C, and the reaction time is 10h to 30h.

7. The method for preparing the anti-contamination blood gas detection reagent card according to claim 6, characterized in that: The reaction temperature is 25° C. to 37° C., and the reaction time is 12 h to 24 h.

8. The method for preparing the anti-contamination blood gas detection reagent card according to any one of claims 1 to 4, characterized in that: The sensor is a potassium ion sensor.

9. The method for preparing the anti-contamination blood gas detection reagent card according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: placing a blood gas detection reagent card plastic part in a mixed solution containing 0.8 mg / mL to 2.0 mg / mL tannic acid and 8 mg / mL to 10 mg / mL silver chloride with a pH of 7 to 7.5, reacting at 25°C to 37°C for 12h to 24h, washing with ultrapure water, drying, and then spot-coating a potassium ion sensor on the surface of the blood gas detection reagent card plastic part.

10. The contamination-proof blood gas detection reagent card prepared by the preparation method according to any one of claims 1 to 9.

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