Method and system for accelerating cell differentiation based on terahertz irradiation of nerve cells

Abstract

The invention provides a method and system for accelerating cell differentiation based on terahertz irradiation of nerve cells, and relates to the technical field of terahertz, and the method comprises the following steps: building a working platform for terahertz irradiation of nerve cells; terahertz is utilized to irradiate target nerve cells based on the working platform. The method provided by the invention can regulate the proliferation and differentiation of nerve cells, accelerate the differentiation of nerve cells and enhance the connection between nerve cells by using a proper dose of irradiation stimulation. The nerve cells can be promoted to differentiate after being irradiated by a proper dose of terahertz, so that the connection between the nerve cells is enhanced and increased, and a certain intervention effect on nervous system diseases is achieved. Therefore, along with the continuous development of cognitive neuroscience in recent years, many non-drug nerve intervention means are generated, and the method for regulating the differentiation of the nerve cells by adopting the appropriate dosage of terahertz wave stimulation is an effective and reliable way for enhancing and increasing the connection between the nerve cells and intervening the faster and better development of the treatment of nervous system diseases.

Description

Technical Field

[0001] The present invention relates to the field of terahertz technology, and in particular to a method and system for accelerating cell differentiation based on terahertz irradiation of nerve cells. Background Art

[0002] Brain synaptic connections are the basis for the brain to store and remember information. Increasing the number of synaptic connections in the brain can enhance our memory. The human brain has about 86 billion neurons, and each neuron is covered with synapses, which enable them to "link" to other neurons. This "link" is not actually connected, but it forms a structure such as synapses to transmit electrical signals from one neuron to the next neuron. It is of great significance to study ways and methods that can help the brain increase the number of neuronal synapses, promote healthy brain growth, and improve memory.

[0003] Modern terahertz biology research shows that all molecules or atoms in life on Earth will emit "terahertz waves" due to thermal vibrations. The light waves emitted by all life activities in nature, such as animals and plants on Earth, are terahertz waves. Because the vibration and rotation energy levels of most biological macromolecules are in the terahertz band, terahertz can resonate with biological macromolecules, activate dormant cells in the body, and repair damaged cells. Therefore, external terahertz radiation will have an impact on the organization and life activities of organisms, which is called the terahertz biological effect. Terahertz biology has currently been applied in biology, medicine and other fields.

[0004] Based on this, how to increase the differentiation rate of neural cells has become a technical problem that needs to be solved urgently. Summary of the invention

[0005] In order to overcome the shortcomings of the prior art, the purpose of the present invention is to provide a method and system for accelerating cell differentiation of nerve cells based on terahertz irradiation. By using appropriate doses of irradiation stimulation, the proliferation and differentiation of nerve cells can be regulated, the differentiation of nerve cells can be accelerated, and the connection between nerve cells can be enhanced.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] A method for accelerating cell differentiation of neural cells based on terahertz irradiation, comprising:

[0008] Build a working platform for terahertz irradiation of neural cells;

[0009] Based on the working platform, terahertz is used to irradiate target neural cells.

[0010] Preferably, after irradiating the target neural cells with terahertz, the method further comprises:

[0011] The irradiated target neural cells are inoculated and cultured under a preset environment;

[0012] The target neural cells were cultured and the cells were characterized for statistical analysis.

[0013] Preferably, the target nerve cells are carried by an external carrying device.

[0014] Preferably, the carrying device is a micro-flow cell, and the inner cavity thickness of the micro-flow cell is 100 μm.

[0015] Preferably, the terahertz irradiation frequency is 2.52 THz, the irradiation power is 35 mW, and the irradiation time is 10 min.

[0016] Preferably, the working platform emits terahertz light through a built-in terahertz gas laser.

[0017] A system for accelerating cell differentiation based on terahertz irradiation of neural cells, comprising:

[0018] A platform building unit, used to build a working platform for terahertz irradiation of neural cells;

[0019] The irradiation unit is used to irradiate the target neural cells using terahertz based on the working platform.

[0020] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0021] The present invention provides a method and system for accelerating cell differentiation based on terahertz irradiation of nerve cells, the method comprising: building a working platform for terahertz irradiation of nerve cells; based on the working platform, irradiating the target nerve cells with terahertz. The present invention utilizes appropriate dose irradiation stimulation to regulate the proliferation and differentiation of nerve cells, accelerate the differentiation of nerve cells, and enhance the connection between nerve cells. Nerve cells can be promoted to differentiate by appropriate dose terahertz irradiation, thereby strengthening and increasing the connection between nerve cells, and playing a certain role in intervention in nervous system diseases. Therefore, with the continuous development of cognitive neuroscience in recent years, many non-drug neural intervention methods have been produced. The use of appropriate dose terahertz wave stimulation methods to regulate the differentiation of nerve cells is an effective and reliable way to enhance and increase the connection between nerve cells and intervene in the treatment of nervous system diseases faster and better. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0023] Figure 1 A flow chart of a method provided by an embodiment of the present invention;

[0024] Figure 2 A schematic diagram of the working platform structure provided by an embodiment of the present invention;

[0025] Figure 3 A schematic diagram of the structure of a load-bearing device provided in an embodiment of the present invention;

[0026] Figure 4 Microscopic images and cell viability images of neural cell differentiation after terahertz irradiation provided in an embodiment of the present invention;

[0027] Figure 5 A scanning electron microscope image of neural cell differentiation after terahertz irradiation provided in an embodiment of the present invention;

[0028] Figure 6 This is a confocal fluorescence imaging diagram of neural cell differentiation after terahertz irradiation provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The purpose of the present invention is to provide a method and system for accelerating cell differentiation of nerve cells based on terahertz irradiation. By using appropriate doses of irradiation stimulation, the proliferation and differentiation of nerve cells can be regulated, the differentiation of nerve cells can be accelerated, and the connection between nerve cells can be enhanced.

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0032] Figure 1 A flow chart of a method provided by an embodiment of the present invention, such as Figure 1 As shown, the present invention provides a method for accelerating cell differentiation of neural cells based on terahertz irradiation, comprising:

[0033] Step 100: Building a working platform for terahertz irradiation of neural cells;

[0034] Step 200: Based on the working platform, terahertz is used to irradiate the target neural cells.

[0035] Specifically, this embodiment provides a method for accelerating cell differentiation of neural cells by irradiating neural cells with terahertz radiation, which comprises the following steps:

[0036] 1. Build an experimental platform suitable for terahertz irradiation (such as Figure 2 As shown in the figure, the terahertz gas laser can achieve simultaneous tuning of the output terahertz frequency and power by adjusting the pump light frequency, cavity length and changing the working gas. The laser can achieve long-term stability of output power by matching the power stabilization system. After the terahertz light emitted by the laser is split by the beam splitter, part of the terahertz light is detected by the terahertz detector and used for power stabilization, and the other part of the terahertz light is converged by optical elements and used for the radiation of nerve cells. The suspended nerve cells are then injected into the microfluidic pool (as shown in the figure). Figure 3 shown).

[0037] 2. Place the microfluidic cell carrying the nerve cells under terahertz light, set the irradiation frequency: 2.52THz, irradiation power: 35mW, irradiation time: 10min.

[0038] 3. Such as Figure 4 and Figure 5 As shown, the irradiated neural cells are inoculated and cultured in a suitable environment.

[0039] 4. After culturing the neural cells for 24 hours, characterize the cells for statistical analysis, such as Figure 6 As shown in the figure, the connections between nerve cells after terahertz irradiation for 5min and 10min were significantly enhanced and increased after 24h of culture. This shows that using appropriate terahertz frequency and power, irradiating nerve cells for 5min and 10min, and culturing them under a preset environment can effectively enhance and increase the connections between nerve cells.

[0040] As a stimulus, terahertz waves can have beneficial effects on organisms under certain conditions. However, as a stimulus, high-intensity and long-term exposure to terahertz waves can also be harmful to organisms. Studies have shown that terahertz wave irradiation of nerve cells can enhance and increase the number of connections between nerve cells, which is higher than the control. The effect of terahertz waves is related to the action time, terahertz frequency and power intensity. The effect is more obvious when the irradiation intensity and irradiation time are moderate.

[0041] The present invention utilizes terahertz to irradiate nerve cells to accelerate cell differentiation, is simple and easy to operate, and is convenient for promotion. It is a nervous system regulation intervention measure worthy of further study.

[0042] Corresponding to the above method, this embodiment further provides a system for accelerating cell differentiation based on terahertz irradiation of neural cells, including:

[0043] A platform building unit, used to build a working platform for terahertz irradiation of neural cells;

[0044] The irradiation unit is used to irradiate the target neural cells using terahertz based on the working platform.

[0045] The beneficial effects of the present invention are as follows:

[0046] The present invention utilizes appropriate dose irradiation stimulation to regulate the proliferation and differentiation of nerve cells, accelerate the differentiation of nerve cells, and enhance the connection between nerve cells. Nerve cells can be promoted to differentiate by appropriate dose terahertz irradiation, thereby strengthening and increasing the connection between nerve cells, and playing a certain role in intervention in nervous system diseases. Therefore, with the continuous development of cognitive neuroscience in recent years, many non-drug neural intervention methods have been produced. The use of appropriate dose terahertz wave stimulation methods to regulate the differentiation of nerve cells is an effective and reliable way to enhance and increase the connection between nerve cells and intervene in the treatment of nervous system diseases faster and better.

[0047] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the system disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0048] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A method for accelerating cell differentiation of neural cells based on terahertz irradiation, characterized in that: include: Build a working platform for terahertz irradiation of neural cells; Based on the working platform, terahertz is used to irradiate target neural cells.

2. The method for accelerating cell differentiation of neural cells based on terahertz irradiation according to claim 1, characterized in that: After irradiating the target neural cells with terahertz, it also includes: The irradiated target neural cells are inoculated and cultured under a preset environment; The target neural cells were cultured and the cells were characterized for statistical analysis.

3. The method for accelerating cell differentiation of neural cells based on terahertz irradiation according to claim 1, characterized in that: The target nerve cells are carried by an external carrying device.

4. The method for accelerating cell differentiation of neural cells based on terahertz irradiation according to claim 1, characterized in that: The carrying device is a micro-flow cell, and the inner cavity thickness of the micro-flow cell is 100 μm.

5. The method for accelerating cell differentiation of neural cells based on terahertz irradiation according to claim 1, characterized in that: The terahertz irradiation frequency is 2.52 THz, the irradiation power is 35 mW, and the irradiation time is 10 min.

6. The method for accelerating cell differentiation of neural cells based on terahertz irradiation according to claim 1, characterized in that: The working platform emits terahertz light through a built-in terahertz gas laser.

7. A system for accelerating cell differentiation of neural cells based on terahertz irradiation, characterized in that: include: A platform building unit, used to build a working platform for terahertz irradiation of neural cells; The irradiation unit is used to irradiate the target neural cells using terahertz based on the working platform.

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