Automatic measuring device and method for plant respiration rate

Abstract

The invention relates to the technical field of plant respiration determination, in particular to an automatic determination device and method for plant respiration rate. The device comprises: a reaction bottle (1); an indicator container (3) for containing an indicator (2); a titrating solution container (5) for containing a titrating solution (4); the basket (7) is used for placing plant seeds (6), the basket (7) is fixedly connected with the reaction bottle (1), and at least one part of the basket (7) is arranged in the reaction bottle (1); the rotary electric oscillation table (8) is used for driving the reaction bottle (1) to oscillate synchronously with the rotary electric oscillation table (8); and the controller (9) is used for controlling the oscillation time and the oscillation amplitude of the rotary electric oscillation table (8). According to the device, the working intensity of experimenters is reduced, the working efficiency is improved, the experiment cost is reduced, the problems of large measurement error and non-ideal repeatability caused by manual operation are avoided, and the measurement accuracy is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant respiration measurement, and in particular to an automatic measurement device and method for plant respiration rate. Background Art

[0002] Respiration rate is also called respiratory intensity, which indicates the number of milligrams (or microliters) of oxygen consumed or carbon dioxide released per gram of living tissue (fresh weight, dry weight, nitrogen content, etc.) per hour. The magnitude of the respiration rate can reflect the strength of a certain organism's metabolic activity. Respiration rate is one of the important indicators of the strength of plant life activities and is often used in plant physiology research and agricultural production practice.

[0003] There are many methods for determining the respiration rate of plant seeds, including the desiccator method, micro-decompression method, oxygen electrode method, infrared analysis method, small basket method, etc. The small basket method has a wide range of applicability due to its simple operation, simple equipment, and low experimental cost.

[0004] The traditional plant respiration rate determination device is to add a three-hole rubber stopper to a wide-mouth bottle 1. A drying tube filled with soda lime is inserted into one hole to absorb CO2 in the air to ensure that there is no CO2 in the air entering the respiration bottle when measuring respiration; a thermometer is inserted into one hole; and the other hole is used for titration. Usually, it is plugged tightly with a small rubber stopper. A small hook is installed under the bottle to hang a small basket made of nylon window screen for holding plant materials. When using the small basket method to determine the respiration rate of plants, the blank titration value and the titration value of the plant sample are first determined, and then the two titration values ​​are used to calculate the plant respiration rate.

[0005] The Chinese patent application with application number 202223060263.0 provides an experimental device for measuring the respiration rate of plants, including a respiration bottle and a sealing cover for increasing the sealing of the respiration bottle. A small basket tube that can be set at the bottle mouth is provided in the respiration bottle, and a support ear is provided at the end of the small basket tube to prevent the small basket tube from falling into the respiration bottle. A plurality of breathing holes are evenly opened on the small basket tube, and an air inlet is provided on the sealing cover, and an auxiliary drying tube for absorbing carbon dioxide is provided on the air inlet. The purpose is to improve the smoothness of operation so that the iron hook of the traditional small basket is easy to fall off or scratch the fingers.

[0006] The traditional plant respiration rate measuring device and the experimental device for measuring the plant respiration rate provided by the Chinese patent application with application number 202223060263.0 are both manually operated by experimenters during the measurement process, resulting in large errors. When measuring multiple plant samples, the repeatability is not ideal, the labor cost is high, the work intensity of the experimenters is high, and the efficiency is low. Summary of the invention

[0007] The purpose of the present invention is to overcome the problems of existing plant respiration rate measuring devices, which are all manually operated by experimenters during the measurement process, resulting in large measurement errors, unsatisfactory repeatability, high labor costs, high work intensity for experimenters and low experimental efficiency, and thus propose an automatic measuring device for plant respiration rate.

[0008] In order to solve the above technical problems, the technical solution of the present invention provides an automatic measuring device for plant respiration rate, comprising:

[0009] Reaction bottle 1;

[0010] An indicator container 3 for containing the indicator 2;

[0011] a titration liquid container 5 for containing the titration liquid 4;

[0012] A basket 7 for placing plant seeds 6, wherein the basket 7 is fixedly connected to the reaction bottle 1, and at least a part of the basket 7 is placed in the reaction bottle 1;

[0013] A rotary electric oscillating table 8, used to drive the reaction bottle 1 to oscillate synchronously therewith; and

[0014] The controller 9 is used to control the oscillation time and oscillation amplitude of the rotary electric oscillating table 8 .

[0015] As an improvement of the above device, the indicator 2 is phenolphthalein.

[0016] As an improvement of the above device, the titration solution 4 is oxalic acid solution.

[0017] As an improvement of the above device, the device further comprises: a reaction bottle cover, wherein the reaction bottle cover cooperates with the reaction bottle 1 to form a closed container.

[0018] As an improvement of the above device, the reaction bottle cover comprises: an inner cover 10, located on the top of the basket 7; and an outer cover 11, respectively connected to the opening of the reaction bottle 1 and the outer edge of the inner cover 10.

[0019] As an improvement to the above device, the cross-sections of the inner cover 10 and the basket 7 are both circular, and the diameter of the inner cover 10 is equal to or smaller than the diameter of the basket 7 .

[0020] As an improvement of the above device, the device also includes: an indicator injection pump 12, which is connected to the indicator container 3 and the reaction bottle 1 respectively, and is used to inject the indicator 2 into the reaction bottle 1; and a titrant injection pump 13, which is connected to the titrant container 5 and the reaction bottle 1 respectively, and is used to inject the titrant 4 into the reaction bottle 1; wherein the pumping time and pumping speed of the indicator injection pump 12 and the titrant injection pump 13 are controlled by the controller 9.

[0021] As an improvement of the above device, the device also includes a color sensor 14, which is arranged near the bottom of the reaction bottle 1 and is used to monitor the color of the liquid inside the reaction bottle 1; the controller 9 controls the titration solution injection pump 13 to be turned off based on the monitoring signal of the color sensor 14.

[0022] As an improvement of the above-mentioned device, the controller 9 includes a start module, a control module, a prompt module, a titration module and a calculation module; when the start module is started for ten minutes, the control module is used to control the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude for one minute; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the start module stops one hour after it is started; the prompt module emits a prompt sound after the stop; when the titration module is started, the control module is used to control the indicator injection pump 12 to start working, inject a predetermined amount of the indicator 2 into the reaction bottle 1, and then control the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude; during the oscillation process, the titration solution injection pump 13 is controlled to inject the titration solution 4 into the reaction bottle 1 until the color sensor 14 senses a preset color and stops; the calculation module calculates the plant respiration rate based on the amount of the titration solution 4.

[0023] To achieve another object of the present invention, the present invention also provides a method for automatically measuring plant respiration rate, the method comprising the following steps:

[0024] Injecting barium hydroxide solution 15 into the reaction bottle 1;

[0025] Starting the start module;

[0026] When the starting module is started for ten minutes, the control module controls the rotary electric oscillating table 8 to oscillate for one minute according to the predetermined oscillation amplitude; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the starting module stops one hour after being started;

[0027] The prompt module emits a prompt sound after the stop;

[0028] Open the inner cover 10, take out the plant seeds 6, and cover the inner cover 10;

[0029] Starting the titration module;

[0030] When the titration module is started, the control module controls the indicator injection pump 12 to start working, injects a predetermined amount of the indicator 2 into the reaction bottle 1, and then controls the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude; during the oscillation process, the titration liquid injection pump 13 is controlled to inject the titration liquid 4 into the reaction bottle 1 until the color sensor 14 senses a preset color and stops;

[0031] The calculation module calculates the plant respiration rate based on the amount of the titration solution 4.

[0032] The advantages of the present invention are that the automatic measuring device for plant respiration rate provided by the present invention is much more convenient to measure plant respiration rate than traditional manual measurement. By setting the program, it only needs to start, and automatic operation is realized. The work intensity of the experimenter is greatly reduced, the work efficiency is improved, the cost of the experiment is reduced, and the problems of large measurement errors and unsatisfactory repeatability caused by manual operation are avoided, thereby improving the accuracy of the measurement. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 A schematic diagram of an automatic device for measuring plant respiration rate provided by the present invention.

[0034] Figure ID

[0035] 1. Reaction bottle 2. Indicator 3. Indicator container

[0036] 4. Titration solution 5. Titration solution container 6. Plant seeds

[0037] 7. Basket 8. Rotary electric oscillating table 9. Controller

[0038] 10. Inner cover 11. Outer cover 12. Indicator injection pump

[0039] 13. Titration solution injection pump 14. Color sensor 15. Barium hydroxide solution

[0040] 16. Nylon bag DETAILED DESCRIPTION

[0041] The technical solution provided by the present invention is further illustrated below in conjunction with embodiments.

[0042] The automatic measuring device for plant respiration rate provided by the present invention measures the plant respiration rate using the small basket method, and the measuring principle is as follows:

[0043] 1) Blank titration

[0044] Pull out the small rubber stopper on the titration hole, use the alkaline burette to accurately add a specific concentration of Ba(OH)2 solution into the wide-mouth bottle, and then plug the titration hole. Shake the wide-mouth bottle for a while. After all the CO2 in the bottle is absorbed, pull out the small rubber stopper and add phenolphthalein, insert the acid burette into the hole, and perform blank titration with a specific concentration of oxalic acid solution until the red color just disappears. Record the amount of oxalic acid solution (mL), which is the blank titration value.

[0045] 2) Determination of titration value of plant samples

[0046] Pour out the waste liquid, wash the wide-mouth bottle with tap water first, then with freshly boiled (to drive CO2 in the water) and cooled distilled water, add the same amount of Ba(OH)2 solution to the bottle, take the plant to be tested, weigh it at the same time, put it in a small basket, open the rubber stopper, quickly hang it on the small hook of the rubber stopper, plug the stopper (when adding samples, try to prevent indoor air and exhaled gas from the mouth from entering the bottle), and start recording the time. Then shake it gently several times to break the BaCO3 film on the surface of the solution, which is conducive to the full absorption of CO2. After the predetermined time, gently open the bottle stopper, quickly take out the small basket, and immediately re-plug it. Shake the wide-mouth bottle fully so that the CO2 in the bottle is completely absorbed, pull out the small rubber stopper, add the same amount of phenolphthalein, and titrate with oxalic acid as before. Write down the amount of oxalic acid, which is the titration value of the plant sample.

[0047] 3) Calculate the respiration rate of plant samples

[0048] After the experiment, the remaining alkali solution is titrated with oxalic acid solution of known concentration. The amount of CO2 released during plant respiration can be calculated from the difference between the consumption of oxalic acid solution by the blank and the sample. The respiration rate of the plant sample can be calculated using the following formula:

[0049]

[0050] Example 1

[0051] like Figure 1 As shown, this embodiment provides an automatic measuring device for plant respiration rate, comprising:

[0052] Reaction bottle 1;

[0053] An indicator container 3 for containing the indicator 2;

[0054] a titration liquid container 5 for containing the titration liquid 4;

[0055] A basket 7 for placing plant seeds 6, wherein the basket 7 is fixedly connected to the reaction bottle 1, and at least a part of the basket 7 is placed in the reaction bottle 1;

[0056] A rotary electric oscillating table 8, used to drive the reaction bottle 1 to oscillate synchronously therewith; and

[0057] The controller 9 is used to control the oscillation time and oscillation amplitude of the rotary electric oscillating table 8 .

[0058] Preferably, the indicator 2 is phenolphthalein.

[0059] Preferably, the titration solution 4 is oxalic acid solution.

[0060] Preferably, the device further comprises: a reaction bottle cover, wherein the reaction bottle cover cooperates with the reaction bottle 1 to form a closed container.

[0061] Preferably, the reaction bottle cover comprises: an inner cover 10 located at the top of the basket 7; and an outer cover 11 connected to the opening of the reaction bottle 1 and the outer edge of the inner cover 10 respectively.

[0062] Preferably, the cross-sections of the inner cover 10 and the basket 7 are both circular, and the diameter of the inner cover 10 is equal to or smaller than the diameter of the basket 7 .

[0063] Preferably, the device further comprises: an indicator injection pump 12, which is respectively connected to the indicator container 3 and the reaction bottle 1, and is used to inject the indicator 2 into the reaction bottle 1; and a titrant injection pump 13, which is respectively connected to the titrant container 5 and the reaction bottle 1, and is used to inject the titrant 4 into the reaction bottle 1; wherein the pumping time and pumping speed of the indicator injection pump 12 and the titrant injection pump 13 are controlled by the controller 9.

[0064] Preferably, the device further comprises a color sensor 14 , which is arranged near the bottom of the reaction bottle 1 and is used to monitor the color of the liquid inside the reaction bottle 1 ; the controller 9 controls the titration solution injection pump 13 to be turned off based on the monitoring signal of the color sensor 14 .

[0065] Preferably, the controller 9 includes a start module, a control module, a prompt module, a titration module and a calculation module; when the start module is started for ten minutes, the control module is used to control the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude for one minute; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the start module stops one hour after it is started; the prompt module emits a prompt sound after the stop; when the titration module is started, the control module is used to control the indicator injection pump 12 to start working, inject a predetermined amount of the indicator 2 into the reaction bottle 1, and then control the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude; during the oscillation process, the titration solution injection pump 13 is controlled to inject the titration solution 4 into the reaction bottle 1 until the color sensor 14 senses a preset color and stops; the calculation module calculates the plant respiration rate based on the amount of the titration solution 4.

[0066] Example 2

[0067] This embodiment provides a method for automatically measuring plant respiration rate, which is implemented based on the device provided in Example 1. The method includes the following steps:

[0068] Injecting barium hydroxide solution 15 into the reaction bottle 1;

[0069] Starting the start module;

[0070] When the starting module is started for ten minutes, the control module controls the rotary electric oscillating table 8 to oscillate for one minute according to the predetermined oscillation amplitude; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the starting module stops one hour after being started;

[0071] The prompt module emits a prompt sound after the stop;

[0072] Open the inner cover 10, take out the plant seeds 6, and cover the inner cover 10;

[0073] Starting the titration module;

[0074] When the titration module is started, the control module controls the indicator injection pump 12 to start working, injects a predetermined amount of the indicator 2 into the reaction bottle 1, and then controls the rotary electric oscillating table 8 to oscillate according to a predetermined oscillation amplitude; during the oscillation process, the titration liquid injection pump 13 is controlled to inject the titration liquid 4 into the reaction bottle 1 until the color sensor 14 senses a preset color and stops;

[0075] The calculation module calculates the plant respiration rate based on the amount of the titration solution 4.

[0076] The working process of this embodiment is to first place the reaction bottle 1 on the rotary electric shaking table 11, add the barium hydroxide solution 15 into the reaction bottle 1, cover the outer cover 11, take out the nylon bag 16 containing the plant seeds 6 and put it into the basket 7, then cover the inner cover 10, and click the start button on the controller 9, and the instrument will automatically complete the experiment.

[0077] Specific operating principle: 10 minutes after the instrument starts, the rotary electric oscillating table 8 oscillates for 1 minute and then stops. This action is performed every 10 minutes to better absorb carbon dioxide. After 1 hour, this action stops and the controller 9 will sound a prompt. At this time, open the inner cover 10, take out the nylon bag 16 with the plant seeds 6, cover the inner cover 10, click titration on the controller 9, and the instrument will automatically run. After the operation is completed, the experimental results will be given.

[0078] The specific operating principle is: after clicking the titration button, the indicator injection pump 12 starts, and the indicator 2 is added to the reaction bottle 1. The indicator 2 of this embodiment uses a phenolphthalein indicator. Then the rotary electric oscillating table 8 is started, and the titration solution injection pump 13 is started. The titration solution 4 in this embodiment uses an oxalic acid solution. When the titration reaches the set color, the color sensor 14 will send a signal, and the titration solution injection pump 13 will stop titrating. The software calculates the amount of oxalic acid solution used, and the plant respiration rate can also be calculated. By writing software and downloading it to the PLC all-in-one module, the above steps can be automatically and accurately controlled.

[0079] From the above specific description of the present invention, it can be seen that the automatic measuring device for plant respiration rate provided by the present invention is much more convenient to measure plant respiration rate than the traditional manual measurement. By setting the program, it only needs to start, and the automatic operation is realized. The work intensity of the experimenter is greatly reduced, the work efficiency is improved, the cost of the experiment is reduced, and the problems of large measurement error and unsatisfactory repeatability caused by manual operation are avoided, thereby improving the accuracy of the measurement.

[0080] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the embodiments, it should be understood by those skilled in the art that any modification or equivalent replacement of the technical solutions of the present invention does not depart from the spirit and scope of the technical solutions of the present invention and should be included in the scope of the claims of the present invention.

Claims

1. An automatic measuring device for plant respiration rate, characterized in that: include: Reaction bottle (1); an indicator container (3) for containing the indicator (2); a titration liquid container (5) for containing a titration liquid (4); A basket (7) for placing plant seeds (6), wherein the basket (7) is fixedly connected to the reaction bottle (1) and at least a portion of the basket (7) is placed in the reaction bottle (1); A rotary electric oscillating table (8) for driving the reaction bottle (1) to oscillate synchronously therewith; and A controller (9) is used to control the oscillation time and oscillation amplitude of the rotary electric oscillating table (8).

2. The automatic measuring device for plant respiration rate according to claim 1, characterized in that: The indicator (2) is phenolphthalein.

3. The automatic measuring device for plant respiration rate according to claim 1, characterized in that: The titration solution (4) is oxalic acid solution.

4. The automatic measuring device for plant respiration rate according to claim 1, characterized in that: Also includes: A reaction bottle cap, wherein the reaction bottle cap cooperates with the reaction bottle (1) to form a closed container.

5. The automatic measuring device for plant respiration rate according to claim 4, characterized in that: The reaction bottle cap comprises: an inner cover (10) located on top of the basket (7); and The outer cover (11) is respectively connected to the opening of the reaction bottle (1) and the outer edge of the inner cover (10).

6. The automatic measuring device for plant respiration rate according to claim 5, characterized in that: The cross sections of the inner cover (10) and the basket (7) are both circular, and the diameter of the inner cover (10) is equal to or smaller than the diameter of the basket (7).

7. The automatic measuring device for plant respiration rate according to claim 6, characterized in that: Also includes: an indicator injection pump (12), which is respectively connected to the indicator container (3) and the reaction bottle (1), and is used to inject the indicator (2) into the reaction bottle (1); and A titrant injection pump (13) is connected to the titrant container (5) and the reaction bottle (1) respectively, and is used to inject the titrant (4) into the reaction bottle (1); wherein the pumping time and pumping speed of the indicator injection pump (12) and the titrant injection pump (13) are controlled by a controller (9).

8. The automatic measuring device for plant respiration rate according to claim 7, characterized in that: It also includes a color sensor (14), which is arranged near the bottom of the reaction bottle (1) and is used to monitor the color of the liquid inside the reaction bottle (1); The controller (9) controls the titration liquid injection pump (13) to be turned off based on the monitoring signal of the color sensor (14).

9. The automatic measuring device for plant respiration rate according to claim 8, characterized in that: The controller (9) comprises a starting module, a control module, a prompting module, a titration module and a calculation module; When the starting module is started for ten minutes, the control module is used to control the rotary electric oscillating table (8) to oscillate for one minute according to a predetermined oscillation amplitude; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the starting module stops one hour after being started; The prompt module emits a prompt sound after the stop; When the titration module is started, the control module is used to control the indicator injection pump (12) to start working, inject a predetermined amount of the indicator (2) into the reaction bottle (1), and then control the rotary electric oscillating table (8) to oscillate according to a predetermined oscillation amplitude; during the oscillation process, the titration liquid injection pump (13) is controlled to inject the titration liquid (4) into the reaction bottle (1), and the operation stops when the color sensor (14) senses a preset color; The calculation module calculates the plant respiration rate based on the amount of the titration solution (4).

10. A method for automatically measuring plant respiration rate, implemented based on the automatic measuring device for plant respiration rate according to claim 9, the method comprising the following steps: Injecting a barium hydroxide solution (15) into the reaction flask (1); Starting the start module; When the starting module is started for ten minutes, the control module controls the rotary electric oscillating table (8) to oscillate for one minute according to a predetermined oscillation amplitude; and after the oscillation stops, the oscillation is repeated for one minute every ten minutes until the starting module stops one hour after being started; The prompt module emits a prompt sound after the stop; Open the inner cover (10), take out the plant seeds (6), and cover the inner cover (10); Starting the titration module; When the titration module is started, the control module controls the indicator injection pump (12) to start working, injects a predetermined amount of the indicator (2) into the reaction bottle (1), and then controls the rotary electric oscillating table (8) to oscillate according to a predetermined oscillation amplitude; during the oscillation process, controls the titration liquid injection pump (13) to inject the titration liquid (4) into the reaction bottle (1), and stops when the color sensor (14) senses a preset color; The calculation module calculates the plant respiration rate based on the amount of the titration solution (4).

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