Method for promoting germination of siraitia grosvenorii seeds
Through disinfectant treatment and gibberellin soaking, the problems of low germination rate of Luohan fruit seeds and hard seed shells are solved, efficient seed germination and seedling growth are achieved, the operation process is simplified, and the germination rate and seedlings of Luohan fruit seeds are improved. Growth conditions ensure the continuation of high-quality species sources.
Patent Information
- Application Number
- CN202411439686.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-15
- Publication Date
- 2025-01-17
AI Technical Summary
In the prior art, the germination rate of Luohan fruit seeds is low and the seed shell is hard, and it is difficult to dehull artificially, resulting in low germination rate, and asexual reproduction leads to a single genotype and a decrease in quality, making it difficult to achieve large-scale production.
After the Luohan fruit seeds are treated with disinfectant, soak them in a solution containing gibberellin for 12 to 36 hours. Combined with appropriate temperature and humidity conditions, seeds are directly sown to promote germination, avoid dehulling treatment, and improve germination rate and seedling growth.
It significantly improves the germination rate of Luohan fruit seeds, ensures the integrity of seeds, simplifies the operation process, reduces costs, and improves the growth of seedlings, providing scientific basis and technical means to ensure the continuation of high-quality seed sources.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of plant seed germination, and in particular relates to a method for improving the germination of monk fruit seeds and an application thereof. Background Art
[0002] Monk fruit ( Siraitia grosvenorii ) is a dioecious perennial vine of the Cucurbitaceae family, also known as the light fruit turtle, the golden invincible, the lahan fruit, etc. Monk fruit is also one of the first batch of precious Chinese medicinal materials for both medicine and food, with the effects of clearing heat and moistening the lungs, relieving sore throat and opening the voice, moistening the intestines and relieving constipation, etc., and is known as the "Oriental Magic Fruit" abroad. Not only that, as one of the three treasures of Guilin, Monk fruit is also a precious medicinal material unique to my country, rich in glucose, fructose and multiple vitamins.
[0003] The fruit of monk fruit is rich in mogroside, which has a pure sweetness and is about 300 times sweeter than sucrose, but has extremely low calories. It is the natural sweetener that is closest to the flavor of sucrose and low in calories. In addition, the mature fruit of monk fruit contains 24 inorganic elements, of which the content of harmful elements to the human body is very low, and the content of elements necessary for the human body is as high as 16, with high content of potassium, magnesium, calcium, iron and manganese. Therefore, the market demand for monk fruit is increasing, especially in some tea beverage industries, which choose to use monk fruit sugar to replace artificial synthetic sugar substitutes such as sucralose. However, since monk fruit is dioecious, the ratio of male to female seed seedlings is 3:7, and only female plants can bear fruit. Therefore, tissue culture is mainly used as a seedling raising method for mass production of monk fruit, and seed seedling raising is rarely used. In agricultural production, frequent use of asexual reproduction will lead to a single genotype of monk fruit, and then the species will degenerate and the quality will decrease. Therefore, seed seedling raising is still the most important breeding method. At present, the methods for promoting the germination of monk fruit seeds on the market generally use shelled seeds for cultivation, but this operation can easily damage the embryo, resulting in a low germination rate. In addition, the shell of monk fruit is hard and difficult to shell manually, which is not suitable for large-scale production. However, this method does not require the seeds to be shelled, and they can be directly sown after disinfection and soaking, which effectively saves time. Summary of the invention
[0004] An object of the present invention is to solve at least the above-mentioned problems and / or disadvantages and to provide at least the advantages which will be described below.
[0005] Another object of the present invention is to provide a method for promoting the germination of Momordica grosvenori seeds, thereby effectively improving the germination rate of the seeds.
[0006] To achieve the above purpose and some other purposes, the present invention adopts the following technical solution.
[0007] A method for promoting the germination of monk fruit seeds, comprising the following steps.
[0008] Step 1: Take monk fruit seeds, put them in disinfectant, leave them for 10 to 20 minutes, take out the seeds and wash them with water to obtain seeds I.
[0009] Step 2: Soak the seeds I in a solution containing gibberellin for 12 to 36 hours to obtain seeds II, and culture the obtained seeds II for 8 to 20 days for germination, wherein the concentration of the gibberellin is 250 to 300 mg / L.
[0010] Preferably, in the method for promoting the germination of monk fruit seeds, in step 1, the disinfectant comprises an ethanol solution with a mass concentration of 70-80%.
[0011] Preferably, in the method for promoting the germination of monk fruit seeds, in step 1, take the monk fruit seeds, put them in a disinfectant, leave them for 10 minutes, take out the seeds and wash them with water to obtain seeds I.
[0012] Preferably, in the method for promoting the germination of monk fruit seeds, in step 2, the seeds I are placed in a solution containing gibberellins for 24 hours to obtain seeds II, and the obtained seeds II are cultured for 11 days to germinate, wherein the concentration of gibberellins is 250 mg / L.
[0013] Preferably, in the method for promoting the germination of monk fruit seeds, in step 2, the culture conditions are a temperature of 25-28° C. and a relative air humidity of 60-80%.
[0014] Preferably, in the method for promoting the germination of monk fruit seeds, the culture conditions are a temperature of 26° C. and an air relative humidity of 60%.
[0015] The technical solution of the present invention has undergone a large number of experiments, and the above steps and parameters are based on the following experimental results:
[0016] Seed soaking experiment: Select healthy and full Momordica grosvenori seeds and treat them with 3 gibberellin concentrations, repeat each treatment 3 times, 10 seeds each time: soak the seeds in gibberellin solution of (250, 300, 350) mg / L for 24 h, and soak the seeds in distilled water as a control. Among them, CK, T1, T2, and T3 represent gibberellin solution of (0, 250, 300, 350) mg / L, respectively. The results show that the germination rate of Momordica grosvenori seeds soaked in 250 mg / L (T1) gibberellin solution for 24 h is significantly higher than that of other groups. See Table 1 for details.
[0017] Table 1 Germination rate of Momordica grosvenori seeds under different gibberellin concentrations Gibberellic acid concentration CK T1 T2 T3 Germination rate (%) 16.67% 53.33% 33.33% 26.67%
[0018] Seedling growth: After the germination, the seedlings were transplanted when they grew to the three-leaf and one-heart stage, and then maintained normal water and fertilizer management. On the 40th day after the germination experiment, three seedlings with the same growth were selected for different gibberellin concentrations, and various growth indicators were measured. Each treatment was repeated three times and the average value was taken. The results showed that the seedlings grown after soaking in 250 mg / L (T1) gibberellin solution had significantly better growth than the seedlings treated with clear water. See Table 2 for details.
[0019] Table 2 Growth of Momordica grosvenori seedlings under different gibberellin concentrations Gibberellic acid concentration CK T1 T2 T3 Plant height (cm) 3.07 3.63 4.73 3.67 Number of roots (strips) 7 9 6 9 Root length (cm) 5.73 6.77 4.63 6.03
[0020] As one of the three treasures of Guilin, artificial propagation of seedlings of Momordica grosvenori has considerable economic benefits. However, the previous seed germination technology is not only complicated, but also easy to cause damage to the seed kernel, thereby reducing the germination rate. The present invention abandons the previous method of removing the seed shell, which saves time and cost well. While ensuring the integrity of the seed kernel, it achieves a short germination time and a germination rate of more than 53%. In addition, the later growth of the Momordica grosvenori seedlings grown under the operation of the present invention is also significantly better than that of the clean water treatment. Therefore, the method has the advantages of simple operation and low cost. While effectively ensuring the continuation of the high-quality seed source of Momordica grosvenori, it also provides important scientific basis and technical means for the genetic breeding of the species.
[0021] Other advantages and features of the present invention will be partially reflected in the following description, and the rest will be understood by those skilled in the art through study and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figures 1 to 11 CK, T1, T2, and T3 shown in the figure represent (0, 250, 300, and 350) mg / L gibberellin solutions, respectively, showing the germination of Momordica grosvenori seeds and the growth of seedlings at a later stage under treatment with different concentrations of gibberellin solutions;
[0023] Figure 1 This is the seed germination status on the 8th day after sowing;
[0024] Figure 2 The germination status of seeds in each treatment was 15 days after sowing;
[0025] Figure 3 The germination status of seeds in each treatment was 17 days after sowing;
[0026] Figure 4 The overall germination of monk fruit seeds after using a transparent plastic box filled with fine river sand;
[0027] Figure 5The germination status of seeds in each treatment was 23 days after sowing;
[0028] Figure 6 The overall germination of monk fruit seeds in the transparent plastic box and the growth of seedlings in the pot;
[0029] Figure 7 Seed germination on the 25th day after sowing;
[0030] Figure 8 The growth of Momordica grosvenori seedlings with different treatments at the three-leaf and one-heart stage;
[0031] Fig. 9 The growth of Momordica grosvenori seedlings under different treatments from the three-leaf one-heart stage to the four-leaf one-heart stage;
[0032] Fig.10 The growth of Momordica grosvenori seedlings with different treatments from the five-leaf and one-heart stage to the six-leaf and one-heart stage;
[0033] Fig.11 This is the root growth of Momordica grosvenori seedlings with different treatments. DETAILED DESCRIPTION
[0034] The present invention is further described in detail below so that ordinary technicians in the field can implement it according to this description.
[0035] The reagents used in the examples are all commercially available products.
[0036] Example 1 (1) Collecting seeds: Collect mature monk fruit at the seed source, clean them, store them in a dry place at room temperature, and remove the seeds before germination; (2) Disinfection and seed soaking: Select healthy, plump, and uniform monk fruit seeds, place them in a 75% ethanol solution, leave them for 10 minutes, take out the seeds and wash them with water 3 to 5 times, and use filter paper to absorb the surface moisture. Then soak the seeds in a 250 mg / L gibberellin solution for 24 hours to allow them to absorb water to saturation; (3) Fine sand culture: Use sterilized fine river sand for culture, and use a clean transparent plastic box of 17×11.9×4.5 cm as a culture dish. Cultivate under normal conditions (temperature 26℃, relative humidity 60%, 12 h light / 12 h dark), and keep the plastic box moist. Finally, the monk fruit seeds will begin to germinate after about 8 days of cultivation, and the germination is basically completed after about 20 days. The monk fruit seedlings with true leaves are transplanted into light loam containing humus. The survival rate of the seedlings is as high as 93%. After transplanting, maintain normal nutrient and water supply, and keep the temperature at 25-28℃, and the monk fruit can continue to grow.
Claims
1. A method for promoting the germination of monk fruit seeds, characterized in that The steps are as follows: (1) Seed collection: Collect mature Luo Han fruits from the seed source, clean them, and store the seeds in a dry place at room temperature; (2) Disinfection and seed soaking: Select healthy, plump, and uniform monk fruit seeds, place them in a 75% ethanol solution, leave them for 10 minutes, take out the seeds, wash them with water 3 to 5 times, and use filter paper to absorb the surface moisture; then soak the seeds in a 250 mg / L gibberellin solution for 24 hours; (3) Substrate preparation: (a) sterilized fine river sand, (b) transparent plastic box, (c) culture medium for transplanting: a mixture of light loam and humus; (4) Fine sand culture: Use step (3) (a) as the germination medium and step (3) (b) as the culture container for germination, culture under normal conditions (temperature 26°C, relative humidity 60%, 12 h light / 12 h dark), and keep the plastic box moist; finally, the monk fruit seeds begin to germinate after about 8 days of culture, and germination is basically completed after about 20 days; (5) Seed germination: The monk fruit seedlings with true leaves are transplanted to step (3) (c). The survival rate of the seedlings is as high as 93%. After transplanting, the normal nutrient and water supply should be maintained, and the temperature should be kept at 25-28°C, so that the monk fruit can continue to grow.
2. A method for promoting the germination of monk fruit seeds according to claim 1, wherein the monk fruit seeds in step (2) must be washed with clean water after disinfection.
3. A method for promoting the germination of monk fruit seeds according to claim 1, wherein the gibberellin solution in step (2) should be stored away from light and preferably prepared and used immediately.