Compound microbial agent for efficiently treating high-concentration organic wastewater as well as preparation method and application of compound microbial agent

Abstract

The invention relates to the technical field of sewage treatment, and provides preparation of a compound microbial agent for efficiently treating high-concentration organic wastewater and an efficient treatment method for the high-concentration organic wastewater. The specific compound microbial agent is prepared from geobacillus stearothermophilus, pseudomonas aeruginosa, microbacterium, Cupriavidus hookworm, Klebsiella oxytoca, mycobacterium, acinetobacter, pichia pastoris, corynespora aspera, bacillus cellulosae and stenotrophomonas microacidophilus. By utilizing the compound microbial agent and through the synergistic effect of bacteria, high-concentration organic wastewater pollutants, especially middle and late period refuse landfill leachate, can be removed with high efficiency, low energy consumption and low cost.

Description

Technical Field

[0001] The invention relates to the technical field of microbial wastewater treatment, and in particular to a composite microbial agent for efficiently treating high-concentration organic wastewater, a preparation method and use thereof. Background Art

[0002] High-concentration organic wastewater is a very complex high-concentration ammonia nitrogen organic wastewater with complex composition, containing a large number of various refractory organic substances (such as various aromatic compounds and humus, etc.), inorganic salts (such as ammonia, carbonate, sulfate, etc.), and metal ions (such as chromium, lead and copper, etc.). As the most important cost expenditure link in the treatment process, the treatment of high-concentration organic wastewater has put forward new demands and challenges for the development of the industry. At present, the combined process of "pretreatment + biological treatment + membrane treatment" is generally used to treat high-concentration organic wastewater. Biological denitrification is a relatively economical and effective method for treating high-concentration organic wastewater. The commonly used biochemical treatment unit is a multi-stage series A / O reactor + MBR membrane. However, in the biochemical treatment process, due to the high ammonia nitrogen concentration and low carbon-nitrogen ratio, the nitrification process requires a lot of aeration, and the aeration energy consumption accounts for about 40-50% of the total energy consumption of the sewage treatment plant. At the same time, a large amount of carbon source needs to be added as an electron donor during the denitrification process, which increases the denitrification cost of high-concentration organic wastewater. In addition, the treatment effect of the biochemical section also directly leads to the nanofiltration and reverse osmosis treatment units in the membrane treatment process producing about 40% of membrane concentrate. For the treatment of reverse osmosis membrane concentrate, the construction and operation costs of existing technologies are relatively high, and there is a lack of mature technical support. How to economically and efficiently remove ammonia nitrogen from high-concentration organic wastewater has become the focus and difficulty of current and future research.

[0003] To achieve the transformation of high-concentration organic wastewater treatment from high energy consumption to energy-saving and high efficiency, the denitrification technology with anaerobic ammonium oxidation (Anamammox) technology as the core is considered to be the most economical and environmentally friendly denitrification technology with the potential for bioenergy recovery. It is suitable for the denitrification treatment of late-stage high-concentration organic wastewater with low C / N, but the residual nitrate nitrogen in its biochemical process eventually leads to the total nitrogen in the effluent not meeting the standard. At present, the most popular and effective nitrate nitrogen treatment process is still heterotrophic denitrification. However, there is a lack of sufficient organic carbon source in the wastewater, and the heterotrophic denitrification process cannot effectively remove nitrate nitrogen. If an organic carbon source is added, the cost of sewage treatment will increase.

[0004] Microorganisms can improve the mass transfer efficiency of pollutants, and more and more high-efficiency microbial agents are widely used in the field of environmental protection. In the traditional composting process, organic matter will undergo a drastic transformation through the addition of functional microbial agents. Sulfur autotrophic denitrification is a process in which sulfur-oxidizing bacteria use inorganic carbon (HCO3 - and CO3 2- etc.), with reducing sulfur sources (H2S, S0, S 2- 、S2O32- ) etc. as electron donors, NO2 - -N or NO3 - -N is reduced to N2. Holistic nitrifying bacteria are one of the key discoveries in the field of microbial nitrogen cycle in recent years. They are widely found in natural environments (such as water bodies, soil, and air) and artificial systems (such as sewage treatment plants, drinking water treatment systems, and recirculating aquaculture systems). They are a type of nitrifying microorganisms with important ecological functions. Research on the environmental ecological distribution, molecular genetics, and microbiological characteristics of holonomic bacteria is increasing rapidly. Studies have found that such microorganisms have the characteristics of high ammonia affinity, low oxygen adaptability, low growth rate, and metabolic diversity. They have a complete genome of key functional enzymes for nitrification (ammonia monooxygenase (AMO) and hydroxylamine dehydrogenase (HAO) for ammonia oxidation, and nitrite oxidoreductase (NXR) for nitrite oxidation. They can complete a two-step nitrification reaction in a single microorganism, converting NH4 + -N is oxidized to NO3 - -N. Bioaugmentation technology introduces microorganisms with specific functions into the biochemical system to increase the concentration of functional microorganisms and the degradation activity of characteristic pollutants in the system. Summary of the invention

[0005] Therefore, the technical problem to be solved by the present invention is to provide a composite microbial agent for efficiently treating high-concentration organic wastewater, a preparation method and a use thereof.

[0006] To this end, the present invention provides the following technical solutions:

[0007] The embodiment disclosed in the present invention relates to a composite microbial agent for efficiently treating high-concentration organic wastewater, comprising:

[0008] Geobacillus stearothermophilus, Pseudomonas aeruginosa, Microbacterium sp., Cupriavidus necator, Klebsiella oxytoca, Mycobacterium sp., Acinetobacter sp., Pichia pastoris, Clavispora lusitaniae, Cellulomonas hominis and Stenotrophomonas acidaminiphila.

[0009] In some embodiments, when the composite microbial agent is in liquid form, the total viable count of the composite microbial agent is 6×10 9 -1×10 10 Pieces / mL.

[0010] In some embodiments, when the composite microbial agent is in solid form, the total viable count of the composite microbial agent is 7×10 9 -4×10 10 Pieces / gram.

[0011] In some embodiments, when preparing the composite microbial agent, the inoculation density of each bacterium is 4×10 9 -1×10 10 pcs / ml;

[0012] And / or, the growth temperature range of the composite microbial agent is 25°C-80°C;

[0013] Optionally, when preparing the composite microbial agent, each bacteria is mixed in equal volume ratio and used as seed liquid, and inoculated at a ratio of 5-10% of the total volume of the fermentation medium.

[0014] In some embodiments, the deposit number of the Geobacillus stearothermophilus is CGMCC No.13968;

[0015] The deposit number of Pseudomonas aeruginosa is CGMCC No.15022;

[0016] The deposit number of Microbacterium sp. is CGMCC No.6530;

[0017] Cupriavidus necator (the deposit number is) CGMCC No.13966;

[0018] The deposit number of Klebsiella oxytoca is CGMCC No.13967;

[0019] The deposit number of Mycobacterium sp. is CGMCC No.6531;

[0020] The deposit number of Acinetobacter sp. is CGMCC No.6532;

[0021] The deposit number of Pichia pastoris is CGMCC No.5960;

[0022] The deposit number of Clavispora lusitaniae is CGMCC No.22799;

[0023] The deposit number of Cellulomonas hominis is CGMCC No. 31471;

[0024] The deposit number of Stenotrophomonas acidaminiphila is CGMCC No.31472;

[0025] The embodiment disclosed in the present invention relates to a method for preparing a composite microbial agent for efficiently treating high-concentration organic wastewater, comprising:

[0026] Select the corresponding strains for activation, fermentation and expansion.

[0027] In some embodiments, the corresponding strains are activated respectively to obtain bacterial suspensions of the respective strains;

[0028] Inoculating the bacterial suspension of each strain into the fermentation medium according to a proportion to carry out fermentation, so as to obtain the mother solution of the composite microbial agent;

[0029] The mother liquid of the composite microbial agent is expanded to obtain a composite microbial agent product.

[0030] The embodiments disclosed in the present invention relate to the use of the composite microbial agent for efficiently treating high-concentration organic wastewater in removing COD, total nitrogen, ammonia nitrogen and / or nitrate nitrogen from high-concentration organic wastewater;

[0031] Optionally, use in the removal of COD, total nitrogen, ammonia nitrogen and / or nitrate nitrogen in urban domestic sewage treatment;

[0032] Optionally, the high-concentration organic wastewater refers to landfill leachate containing heterocyclic aromatic hydrocarbons, humus and / or amide-type complex organic compounds;

[0033] Optionally, the urban domestic sewage refers to urban domestic sewage containing industrial organic wastewater;

[0034] Optionally, the high-concentration organic wastewater contains COD 1000-10000 mg / L, TN 2200-3400 mg / L, and ammonia nitrogen 900-2200 mg / L;

[0035] Optionally, the high-concentration organic wastewater contains 6000-9000 mg / L COD, 2200-3400 mg / L TN, and 2000-2200 mg / L ammonia nitrogen;

[0036] Optionally, the high-concentration organic wastewater contains COD of 7400-9200 mg / L, TN of 2200-2400 mg / L, and ammonia nitrogen of 900-1100 mg / L.

[0037] The embodiment disclosed in the present invention relates to a method for treating high-concentration organic wastewater using the composite microbial agent for efficiently treating high-concentration organic wastewater, comprising:

[0038] Micro-oxygen aeration treatment is adopted, and the composite microbial agent is used under low dissolved oxygen conditions. According to the COD concentration of the high-concentration organic wastewater, the high-efficiency composite microbial agent is added at 0.1-10% v / v of the influent.

[0039] In some embodiments, at least one of the following processes is included:

[0040] (1) Use sequencing batch intermittent water inflow process for treatment:

[0041] 1) inoculating the high-concentration organic wastewater stock solution with a high-efficiency composite microbial agent, stirring, and aerating;

[0042] 2) then discharging a portion of the high-concentration organic wastewater stock solution treated in step 1), and then adding a portion of the untreated high-concentration organic wastewater stock solution, inoculating a high-efficiency composite microbial agent, stirring, and aerating;

[0043] 3) Repeat step 2) at least once;

[0044] (2) Use continuous water inflow process for treatment:

[0045] The high-concentration organic wastewater stock solution is continuously fed with water, and sequentially subjected to anaerobic treatment, anoxic treatment, aerobic treatment and sedimentation treatment; during the anaerobic treatment, the highly efficient composite microbial agent is added;

[0046] Optionally, in anaerobic treatment, the dissolved oxygen in the water is controlled to be maintained at 0.02-0.06 mg / L;

[0047] Optionally, in the anoxic treatment, the dissolved oxygen in the water was controlled to be maintained at 0.3-0.5 mg / L;

[0048] Optionally, in aerobic treatment, the dissolved oxygen in the water is controlled to be maintained at 1.5-2.5 mg / L.

[0049] The technical solution of the present invention has the following advantages:

[0050] 1. The present invention provides a composite microbial agent for efficiently treating high-concentration organic wastewater, comprising: Geobacillus stearothermophilus, Pseudomonas aeruginosa, Microbacterium sp., Cupriavidus necator, Klebsiella oxytoca, Mycobacterium sp., Acinetobacter sp., Pichia pastoris, Clavisporalusitaniae, Cellulomonas hominis and Stenotrophomonas acidophilus. acidaminiphila); the composite microbial agent can treat high-concentration organic wastewater. On the one hand, it can efficiently treat high-concentration organic wastewater. The addition amount of the composite microbial agent is 0.1-10% v / v of the water volume, and the minimum is 0.2% of the total water volume. After the multi-stage A / O biological reaction system is started for 60 days, the COD optimal removal rate reaches more than 80%, the ammonia nitrogen removal rate reaches more than 60%, and the total nitrogen removal rate reaches more than 60%;

[0051] On the other hand, when the COD concentration of the influent load was increased from 1050 mg / L to 9290 mg / L, the removal rates of COD, ammonia nitrogen and total nitrogen remained stable. This indicates that the treatment system is resistant to changes in the concentration of pollutants in high-concentration organic wastewater by adding the composite microbial agent of the present invention, and can stably remove COD and ammonia nitrogen in high-concentration organic wastewater under low dissolved oxygen conditions. Compared with traditional treatment processes, the system energy consumption caused by a large amount of aeration during the treatment process is reduced.

[0052] Biodeposit Information:

[0053] The deposit number of the Geobacillus stearothermophilus is CGMCC No.13968, the deposit unit is the General Microbiology Center of the China Microbiological Culture Collection Administration, the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is April 1, 2017;

[0054] The deposit number of Pseudomonas aeruginosa is CGMCC No. 15022, and the depositor is the General Microbiology Center of the China Microbiological Culture Collection Administration. The deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is December 7, 2017;

[0055] The deposit number of Microbacterium sp. is CGMCC No.6530, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Committee. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is September 6, 2012;

[0056] Cupriavidus necator has a deposit number of CGMCC No.13966, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Administration. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is April 1, 2017;

[0057] The deposit number of Klebsiella oxytoca is CGMCC No.13967, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Committee. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is April 1, 2017;

[0058] The deposit number of Mycobacterium sp. is CGMCC No.6531, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Committee. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is September 6, 2012;

[0059] The deposit number of Acinetobacter sp. is CGMCC No.6532, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Committee. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is September 6, 2012;

[0060] The deposit number of Pichia pastoris is CGMCC No.5960, and the depositor is the General Microbiology Center of China National Microbiological Culture Collection Administration. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is April 9, 2012;

[0061] The deposit number of Clavispora lusitaniae is CGMCC No.22799, and the depositor is the General Microbiology Center of the China Microbiological Culture Collection Administration. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is June 29, 2021;

[0062] The deposit number of Cellulomonas hominis is CGMCC No. 31471, and the depositor is the General Microbiology Center of the China Microbiological Culture Collection Administration. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is July 26, 2024;

[0063] The deposit number of Stenotrophomonas acidaminiphila is CGMCC No.31472. The depository is the General Microbiology Center of China National Microbiological Culture Collection Administration. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Postal Code 100101, and the deposit date is July 26, 2024. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0065] Figure 1 It is the detection result of COD degradation in Example 2 of the present invention;

[0066] Figure 2 is the total nitrogen (TN) degradation test result in Example 2 of the present invention;

[0067] Figure 3 is the ammonia nitrogen (NH4 + -N) Degradation test results;

[0068] Figure 4 is a sewage treatment process flow chart in Example 3 of the present invention;

[0069] Figure 5 It is the detection result of COD degradation in Example 3 of the present invention;

[0070] Figure 6 is the ammonia nitrogen (NH4 + -N) Degradation test results;

[0071] Figure 7 This is the detection result of the degradation of total nitrogen (TN) in Example 3 of the present invention. DETAILED DESCRIPTION

[0072] The following examples are provided for a better understanding of the present invention, but are not intended to limit the best mode of implementation, nor to limit the content and protection scope of the present invention. Any product identical or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior arts shall fall within the protection scope of the present invention.

[0073] If no specific experimental steps or conditions are specified in the examples, the conventional experimental steps or conditions described in the literature in the field can be used. If no manufacturer is specified for the reagents or instruments used, they are all conventional reagent products that can be obtained commercially.

[0074] In the following examples, COD refers to chemical oxygen demand; TN refers to total nitrogen;

[0075] Example 1 Preparation of composite microbial agent

[0076] a. Preparation of composite microbial agent mother solution:

[0077] The following strains were activated respectively:

[0078] The deposit number of Geobacillus stearothermophilus is CGMCC No.13968;

[0079] The deposit number of Pseudomonas aeruginosa is CGMCC No.15022;

[0080] The deposit number of Microbacterium sp. is CGMCC No.6530;

[0081] Cupriavidus necator (the deposit number is) CGMCC No.13966;

[0082] The deposit number of Klebsiella oxytoca is CGMCC No.13967;

[0083] The deposit number of Mycobacterium sp. is CGMCC No.6531;

[0084] The deposit number of Acinetobacter sp. is CGMCC No.6532;

[0085] The deposit number of Pichia pastoris is CGMCC No.5960;

[0086] The deposit number of Clavispora lusitaniae is CGMCC No.22799;

[0087] The deposit number of Cellulomonas hominis is CGMCC No. 31471;

[0088] The deposit number of Stenotrophomonas acidaminiphila is CGMCC No.31472;

[0089] After the activation, the bacterial suspensions of each strain were obtained; the bacterial suspensions of each strain were in a certain proportion (in this embodiment, the inoculation density of each strain was 4×10 9 -1×10 10 The composite bacteria composed of 4 × 10 cells / ml) were inoculated into the activation medium and cultured at 35°C for 24 hours; then the activated composite bacteria were inoculated into the fermentation tank and fermented at 35°C for 18 hours using the fermentation medium to prepare a composite bacterial suspension with a viable bacterial count of ≥4 × 10 9 CFU / mL, that is, the preparation of the composite microbial agent mother solution is complete.

[0090] The activation culture medium, per 1000 mL, is composed of: 18.0 g of glucose, 0.9 g of dipotassium hydrogen phosphate, 0.36 g of magnesium sulfate, 5 g of yeast powder, 1.8 g of peptone, 1.8 g of sodium chloride, and the balance is water.

[0091] The fermentation medium, per 1000 mL, is composed of: 2-5.0 g of sodium alginate, 10-20.0 g of molasses, 0.5-1.0 g of dipotassium hydrogen phosphate, 0.2-0.39 g of magnesium sulfate, 5-10 g of yeast powder, and the balance is water.

[0092] b. Preparation of liquid composite microbial agent products (taking 100L as an example):

[0093] Move a 100-liter plastic white barrel to a place where direct sunlight is exposed outdoors, add about 50L of clean water (well water or tap water, tap water needs to be aired for a period of time to prevent the residual chlorine in the tap water from affecting the growth of bacteria) into the barrel; add the weighed raw materials of the liquid expansion culture medium components and 10L of the composite microbial agent mother liquor into the 100L white barrel, stir evenly with a clean long stick, and then fill the plastic white barrel with water to a volume of 100L (each 100L composition is: yeast powder 200g, molasses 2kg, potassium dihydrogen phosphate 60.0g, magnesium sulfate 12g, rice bran meal 250g, the rest is water), and seal and ferment for 2 days. The total number of viable bacteria in the liquid composite microbial agent product is 6×10 9 -4×10 10 Pieces / mL.

[0094] c. Preparation of solid composite microbial agent products:

[0095] The composite microbial agent mother liquid was inoculated into the solid expansion medium according to 10% of the mass of the solid expansion medium, and fermented at room temperature (25°C) for 48 hours. The total viable count of the solid composite microbial agent product was 7×10 9 -4×10 10 Pieces / gram.

[0096] The solid-state expansion culture medium is composed of: 23wt% soybean meal, 65wt% corn flour, 2wt% molasses, 9wt% rice bran meal, and 1wt% yeast powder. Water is added to the above raw materials, and the material-water ratio is 1.5:1 (mass ratio).

[0097] d. Preparation of solid composite microbial agent working solution (taking 100L as an example):

[0098] Move a 100-liter plastic white barrel to a place where direct sunlight is exposed outdoors, add about 50L of clean water (well water or tap water, tap water needs to be aired for a period of time to prevent the residual chlorine in the tap water from affecting the growth of bacteria) into the barrel; add the weighed raw materials of the expansion culture medium components and 400g of the solid composite microbial agent product (prepared in step c) into the 100L white barrel, stir evenly with a clean long stick, and then fill the plastic white barrel with water to a volume of 100L (each 100L consists of: 200g yeast powder, 2kg molasses, 60.0g dipotassium hydrogen phosphate, 12g magnesium sulfate, 250g rice bran meal, and the rest is water), and seal and ferment for 2 days. The total number of viable bacteria in the solid composite microbial agent working solution product is 6×10 9 -4×10 10 Pieces / mL.

[0099] Example 2 Degradation of high-concentration organic wastewater by composite microbial agent (water inlet method: intermittent water inlet)

[0100] This embodiment uses the original solution of landfill leachate from a certain place for testing, wherein the water quality indicators of the original solution of landfill leachate are: COD 6000-9000mg / L, TN 2200-3400mg / L, and ammonia nitrogen 2000-2200mg / L.

[0101] This embodiment provides a method for treating landfill leachate, including using batch intermittent water inlet, performing timed stirring through a magnetic stirrer and a controller, and collecting dissolved oxygen concentration and pH through a multi-parameter water quality measuring instrument (Multi 3630).

[0102] Operation parameters: temperature: room temperature 30-33°C; hydraulic retention time: 8-10 days, after 9 hours of stirring, stirring was stopped, aeration was performed for 15 hours (aeration was performed by an aeration pump, the aeration pump exhaust volume was 82L / min pressure, and the dissolved oxygen content in the liquid was less than 0.1mg / L), the water inflow was 500mL / day, the water discharge was 500mL / day, and the inoculation amount of the bacterial agent was 10% v / v of the water inflow per day. The bacterial agent was a liquid composite microbial bacterial agent product (prepared in Example 1).

[0103] The process steps are:

[0104] At room temperature of 30-33°C, the original solution of landfill leachate (volume 5L) was placed in a reactor. On day 0, a liquid composite microbial agent product was added, and the inoculation amount was 10% v / v of the original solution of landfill leachate (volume 5L). Then, starting from the next day as day 1, at the same time every day, the treated water in the reactor was first discharged, with a drainage volume of 500mL / day, and then the original solution of landfill leachate was added to the reactor, with a water intake of 500mL / day, and then the liquid composite microbial agent product was added to the reactor, with an inoculation amount of 10% v / v of the water intake. The above drainage-water intake-inoculation operation was continued for 24 days.

[0105] From the 0th to the 24th day, after inoculating the liquid composite microbial agent product, stir for 9 hours every day to mix the water in the upper and lower parts of the reactor evenly, then stop stirring, aerate for 15 hours, aerate by an aeration pump, the aeration pump exhaust volume is 82L / min pressure, and the dissolved oxygen content in the liquid in the reactor is less than 0.1mg / L. Each time water is inflowed and discharged, the COD, TN and ammonia nitrogen in the inflow water (garbage leachate raw liquid) are tested, and the COD, TN and ammonia nitrogen in the water discharged from the reactor are tested.

[0106] The results show that the COD concentration of the landfill leachate changes from the original influent concentration of 6455-8767 mg / L. After the system is continuously operated for 24 days, the lowest effluent COD concentration is 2893 mg / L, and the highest degradation rate is 69.76% ( Figure 1 ); The original total nitrogen concentration of the landfill leachate was 2816-3267 mg / L. After 24 days of continuous operation, the lowest total nitrogen concentration in the effluent was 984 mg / L, and the highest total nitrogen degradation rate was 67.9% ( Figure 2 ); The original concentration of ammonia nitrogen in the leachate was 2071-2409 mg / L. After 24 days of continuous operation, the lowest effluent ammonia nitrogen concentration was 839 mg / L, and the highest ammonia nitrogen degradation rate was 62.0% ( Figure 3 ).

[0107] Example 3 Verification 2 of the degradation effect of composite microbial agent on high-concentration organic wastewater (water inlet method: continuous water inlet)

[0108] This case uses leachate from a landfill to verify the water quality. The main water quality parameters are shown in Table 1.

[0109] Table 1 Water quality characteristics of landfill leachate raw water

[0110] COD(mg / L) <![CDATA[NH4 + -N(mg / L)]]> TN(mg / L) pH 7392-18580 1428-2820 2400-2254 8.3-8.6

[0111] System influent water quality index: The influent water is diluted with the original landfill leachate. The COD concentration of the water entering the system is roughly divided into three concentration ranges according to the system operation time, which are:

[0112] In the first stage, the COD concentration entering the system is controlled at 1056-2462 mg / L, the ammonia nitrogen concentration is controlled at 336-356 mg / L, and the TN concentration is controlled at 336-360 mg / L;

[0113] In the second stage, the COD concentration entering the system is controlled at 3200-4400 mg / L, the ammonia nitrogen concentration is controlled at 356-450 mg / L, and the TN concentration is controlled at 360-464 mg / L;

[0114] In the third stage, the COD concentration entering the system is controlled at 7400-9200 mg / L, the ammonia nitrogen concentration is controlled at 980-1100 mg / L, and the TN concentration is controlled at 980-1120 mg / L.

[0115] System startup: directly add the composite microbial agent (solid composite microbial agent working solution) for efficiently treating high-concentration organic wastewater in Example 1 at a ratio of 5-10% of the influent volume, without adopting the traditional method of adding activated sludge inoculum when starting the biochemical pool.

[0116] This embodiment adopts a conventional wastewater treatment continuous water inlet operation treatment process: the process adopted by the continuous water inlet is as follows Figure 4 , the system includes a sewage tank, an anaerobic tank, an anoxic tank, an aerobic tank and a secondary sedimentation tank connected in sequence, a part of the secondary sedimentation tank is connected to the nitrification tank, a part is connected to the drainage system, the nitrification tank is connected to the anaerobic tank, and is connected to the reactor water inlet pipe through a peristaltic pump. The sewage tank, the anaerobic tank, the anoxic tank, the aerobic tank, the secondary sedimentation tank and the nitrification tank are all conventionally set. In the present embodiment, the number of cells arranged in the bioreactor (anaerobic tank, anoxic tank, aerobic tank) is as follows: along the direction of water flow, the anaerobic tank is arranged with 4 cells of uniform volume along the direction of water flow, and the overflow mode is adopted by height difference control, the anoxic tank is arranged with 3 cells of uniform volume along the direction of water flow by a grid, and the aerobic tank is arranged with 2 cells of uniform volume by a grid along the direction of water flow, and the anoxic tank and the aerobic tank adopt staggered grids to control the water flow to flow in an S-shaped direction. The secondary sedimentation tank is connected to the aerobic tank water inlet pipe through a peristaltic pump, and the nitrification liquid return is connected to the first cell in the anaerobic tank along the water flow direction. In this embodiment, the effective volumes of the sewage tank, anaerobic tank, anoxic tank, aerobic tank, secondary sedimentation tank and nitrification tank are 50 liters, 9.791L, 20.655L, 13.770L, 7.203L and 1.656L respectively. The process steps are as follows:

[0117] (1) The diluted liquid of the original landfill leachate continuously enters the sewage tank, and the influent concentration of the original landfill leachate is divided into three gradients according to the operation time.

[0118] (2) The water in the sewage tank continuously enters the anaerobic tank, and the composite microbial agent product prepared in Example 1 is added to the first compartment along the water flow direction of the anaerobic tank every day. The addition amount is 0.2% v / v of the total water inflow of the anaerobic tank every day, and the dissolved oxygen in the anaerobic tank is maintained at 0.02-0.06 mg / L.

[0119] (3) The water in the anaerobic tank overflows into the anoxic tank, and the dissolved oxygen in the anoxic tank is maintained at 0.3-0.5 mg / L;

[0120] (4) The water in the anoxic pool flows into the aerobic pool, and the dissolved oxygen in the aerobic pool is maintained at 1.5-2.5 mg / L;

[0121] (5) The water in the aerobic tank flows to the secondary sedimentation tank. After the mud and water are separated in the secondary sedimentation tank, part of the treated water is discharged, and a small part enters the nitrification tank for nitrification, and then recirculates into the anaerobic tank

[0122] Operation parameters: Continuous water inflow is adopted. The reactor reflux ratio is 2:1 (reflux ratio is the ratio of reflux volume to water inflow volume, reflux volume is the amount of water returned to the nitrification tank every day, and water inflow is the amount of water entering the anaerobic tank every day):

[0123] The dissolved oxygen in the anaerobic tank is maintained at 0.02-0.06 mg / L, the pH in the tank is maintained at 7.2-8.5, and an air compressor is used for aeration. The specific model is: power: 60W; voltage / frequency: 220v / 50Hz; exhaust volume: 82L / min pressure: >0.035MPa.

[0124] The oxygen supply in the aerobic zone is regulated by adjusting the opening degree of the gas volume regulating valve. The dissolved oxygen in the aerobic zone is 0.02-0.1 mg / L when the system is initially started. With the degradation of COD and ammonia nitrogen in the wastewater, the dissolved oxygen gradually increases and is basically stable between 1.5-2.5 mg / L during operation. The room temperature is controlled at 25°C during operation; the hydraulic retention time is 5-10 days, and the system does not discharge mud during the process.

[0125] Dissolved oxygen concentration and pH were collected by a multi-parameter water quality meter (Multi 3630), and COD, ammonia nitrogen and total nitrogen were measured according to national standard methods. At the same time every day, the COD, ammonia nitrogen and total nitrogen of the water in the anaerobic tank were tested, and the COD, ammonia nitrogen and total nitrogen of the water discharged from the secondary sedimentation tank to the drainage system were tested.

[0126] Depend on Figure 5 It can be seen that during the operation, except for the initial COD removal rate of about 40% when the system was started, the removal rate was always maintained above 80% as the COD of the system influent increased. Figure 6It can be seen that the ammonia nitrogen removal rate has increased from the initial 23-43%. With the stable operation of the system, along with the increase of ammonia nitrogen in the system influent, the ammonia nitrogen removal rate has basically stabilized at more than 65-80%; along with the increase of total nitrogen in the system influent, the total nitrogen removal rate of the system has also basically stabilized at more than 60%, and most of the time the total nitrogen removal rate of the effluent is maintained at around 70% ( Figure 7 ).

[0127] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A composite microbial agent for efficiently treating high-concentration organic wastewater, characterized in that: include: Geobacillus stearothermophilus, Pseudomonas aeruginosa, Microbacterium sp., Cupriavidus necator, Klebsiella oxytoca, Mycobacterium sp., Acinetobacter sp., Pichia pastoris, Clavispora lusitaniae, Cellulomonas hominis and Stenotrophomonas acidaminiphila.

2. The composite microbial agent for efficiently treating high-concentration organic wastewater according to claim 1, characterized in that: When the composite microbial agent is in liquid form, the total viable count of the composite microbial agent is 6×10 9 -1×10 10 Pieces / mL.

3. The composite microbial agent for efficiently treating high-concentration organic wastewater according to claim 1 or 2, characterized in that: When the composite microbial agent is in solid state, the total viable count of the composite microbial agent is 7×10 9 -4×10 10 Pieces / gram.

4. The composite microbial agent for efficiently treating high-concentration organic wastewater according to any one of claims 1 to 3, characterized in that: When preparing the composite microbial agent, the inoculation density of each bacterium is 4×10 9 -1×10 10 pcs / ml; And / or, the growth temperature range of the composite microbial agent is 25°C-80°C; Optionally, when preparing the composite microbial agent, each bacteria is mixed in equal volume ratio and used as seed liquid, and inoculated at a ratio of 5-10% of the total volume of the fermentation medium.

5. The composite microbial agent for efficiently treating high-concentration organic wastewater according to any one of claims 1 to 4, characterized in that: The deposit number of the Geobacillus stearothermophilus is CGMCC No.13968; The deposit number of Pseudomonas aeruginosa is CGMCC No.15022; The deposit number of Microbacterium sp. is CGMCC No.6530; Cupriavidus necator (the deposit number is) CGMCC No.13966; The deposit number of Klebsiella oxytoca is CGMCC No.13967; The deposit number of Mycobacterium sp. is CGMCC No.6531; The deposit number of Acinetobacter sp. is CGMCC No.6532; The deposit number of Pichia pastoris is CGMCC No.5960; The deposit number of Clavispora lusitaniae is CGMCC No.22799; The deposit number of Cellulomonas hominis is CGMCC No. 31471; The deposit number of Stenotrophomonas acidaminiphila is CGMCC No.31472.

6. A method for preparing a composite microbial agent for efficiently treating high-concentration organic wastewater as claimed in any one of claims 1 to 5, characterized in that: include: Select the corresponding strains for activation, fermentation and expansion.

7. The method for preparing the composite microbial agent for efficiently treating high-concentration organic wastewater according to claim 6, characterized in that: Activating the corresponding strains respectively to obtain bacterial suspensions of the strains; Inoculating the bacterial suspension of each strain into the fermentation medium according to a proportion to carry out fermentation, so as to obtain the mother solution of the composite microbial agent; The mother liquid of the composite microbial agent is expanded to obtain a composite microbial agent product.

8. Use of the composite microbial agent for efficiently treating high-concentration organic wastewater according to any one of claims 1 to 5 in removing COD, total nitrogen, ammonia nitrogen and / or nitrate nitrogen from high-concentration organic wastewater; Optionally, use in the removal of COD, total nitrogen, ammonia nitrogen and / or nitrate nitrogen in urban domestic sewage treatment; Optionally, the high-concentration organic wastewater refers to landfill leachate containing heterocyclic aromatic hydrocarbons, humus and / or amide-type complex organic compounds; Optionally, the urban domestic sewage refers to urban domestic sewage containing industrial organic wastewater; Optionally, the high-concentration organic wastewater contains COD 1000-10000 mg / L, TN 2200-3400 mg / L, and ammonia nitrogen 900-2200 mg / L; Optionally, the high-concentration organic wastewater contains 6000-9000 mg / L COD, 2200-3400 mg / L TN, and 2000-2200 mg / L ammonia nitrogen; Optionally, the high-concentration organic wastewater contains COD of 7400-9200 mg / L, TN of 2200-2400 mg / L, and ammonia nitrogen of 900-1100 mg / L.

9. A method for treating high-concentration organic wastewater using the composite microbial agent for efficiently treating high-concentration organic wastewater according to any one of claims 1 to 5, characterized in that: include: Micro-oxygen aeration treatment is adopted, and the composite microbial agent is used under low dissolved oxygen conditions. According to the COD concentration of the high-concentration organic wastewater, the high-efficiency composite microbial agent is added at 0.1-10% v / v of the influent.

10. The method for treating high-concentration organic wastewater with a composite microbial agent for efficiently treating high-concentration organic wastewater according to claim 9, characterized in that: Including at least one of the following processes: (1) Use sequencing batch intermittent water inflow process for treatment: 1) inoculating the high-concentration organic wastewater stock solution with a high-efficiency composite microbial agent, stirring, and aerating; 2) then discharging a portion of the high-concentration organic wastewater stock solution treated in step 1), and then adding a portion of the untreated high-concentration organic wastewater stock solution, inoculating a high-efficiency composite microbial agent, stirring, and aerating; 3) Repeat step 2) at least once; (2) Use continuous water inflow process for treatment: The high-concentration organic wastewater stock solution is continuously fed with water, and sequentially subjected to anaerobic treatment, anoxic treatment, aerobic treatment and sedimentation treatment; during the anaerobic treatment, the highly efficient composite microbial agent is added; Optionally, in anaerobic treatment, the dissolved oxygen in the water is controlled to be maintained at 0.02-0.06 mg / L; Optionally, in the anoxic treatment, the dissolved oxygen in the water was controlled to be maintained at 0.3-0.5 mg / L; Optionally, in aerobic treatment, the dissolved oxygen in the water is controlled to be maintained at 1.5-2.5 mg / L.

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