Calibration curve detection method of fluorescence analyzer

Abstract

The invention discloses a method for detecting a calibration curve by a fluorescence analyzer, which improves the detection precision and accuracy of the fluorescence analyzer and provides a more reliable detection result for guiding production. The establishment process of the calibration curve is simplified, and time and labor cost are saved; the system has strong anti-interference capability and can adapt to different environmental conditions; practical application proves that the curve is effective and practical.

Description

Technical Field

[0001] The invention relates to the field of fluorescence analysis detection, and in particular to a fluorescence analyzer detection calibration curve method. Background Art

[0002] In the prior art, the detection precision and accuracy of fluorescence analysis largely depend on the accuracy of the calibration curve. However, there are many problems with the traditional calibration curve establishment method. For example, the establishment process is complicated, time-consuming, and easily interfered by environmental factors. This leads to insufficient reliability of the detection results. The purpose of the present invention is to provide a new method and system for fluorescence analyzer calibration curve detection to improve the detection precision and accuracy of the fluorescence analyzer, while simplifying the calibration curve establishment process and improving work efficiency. Summary of the invention

[0003] The technical problem to be solved by the present invention is to overcome the defects of the prior art and provide a method for detecting a calibration curve of a fluorescence analyzer.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions: The present invention provides a method for detecting a calibration curve of a fluorescence analyzer, comprising the following method: Step 1: Set the detection conditions and obtain condition 1 and condition 2; Step 2: number each type of sample, and then put the numbered samples into the fluorescence analyzer for analysis under condition 1 and condition 2 respectively, to obtain multiple detection spectra for each type of sample; Step 3: Use the software of the fluorescence analyzer to calculate the intensity of A detection spectra of each type of sample, optimize the calculation results and summarize them into tables and curves; Step 4: Compare the data obtained by the fluorescence analyzer of the mixed sample with the table and the curve. If the comparison result of the sample with the table and the curve is within the error range, it is qualified, otherwise it is unqualified.

[0005] As a preferred technical solution of the present invention, in the condition 1, the tube pressure (KV) is 5-10, the tube flow (UA) is 100-400, the time (seconds) is 60-120, the filter is 1-5, and the sample chamber gas is vacuum; In the condition 2, the tube pressure (KV) is 15-20, the tube flow (UA) is 400-600, the time (seconds) is 60-120, the filter discs are 5-10, and the sample chamber gas is vacuum.

[0006] As a preferred technical solution of the present invention, one or more of Na, Mg, Al, Si, P, and S are added in sequence to the sample tested under condition one as needed, and corresponding analysis data are obtained.

[0007] As a preferred technical solution of the present invention, one or more of K, Ca, Ti, Cr, Mn, and Fe are added in sequence to the sample tested under condition 2 as needed, and corresponding analysis data are obtained.

[0008] As a preferred technical solution of the present invention, the average error of the curve is less than 0.046 and is smaller than the chemical allowable error.

[0009] As a preferred technical solution of the present invention, the step three further includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

[0010] As a preferred technical solution of the present invention, the step three further includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

[0011] Compared with the prior art, the present invention has the following beneficial effects: The detection precision and accuracy of the fluorescence analyzer are improved, providing more reliable detection results to guide production; the calibration curve establishment process is simplified, saving time and labor costs; it has strong anti-interference ability and can adapt to different environmental conditions; through practical application, the effectiveness and practicality of the curve are proved. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 It is a step flow chart of the present invention; Figure 2 It is the fitting curve diagram of the present invention. DETAILED DESCRIPTION

[0013] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0014] In the drawings, the same reference numerals all refer to the same components.

[0015] Embodiment 1: like Figure 1-2 As shown, the present invention provides a method for detecting a calibration curve of a fluorescence analyzer, comprising the following method: Step 1: Set the detection conditions and obtain condition 1 and condition 2; Step 2: number each type of sample, and then put the numbered samples into the fluorescence analyzer for analysis under condition 1 and condition 2 respectively, to obtain multiple detection spectra for each type of sample; Step 3: Use the software of the fluorescence analyzer to calculate the intensity of A detection spectra of each type of sample, optimize the calculation results and summarize them into tables and curves; Step 4: Compare the data obtained by the fluorescence analyzer of the mixed sample with the table and the curve. If the comparison result of the sample with the table and the curve is within the error range, it is qualified, otherwise it is unqualified.

[0016]

[0017] sheet Furthermore, in the condition 1, the tube pressure (KV) is 5, the tube flow (UA) is 100, the time (seconds) is 60-120, the filter is 1, and the sample chamber gas is vacuum; In the condition 2, the tube pressure (KV) is 15, the tube flow (UA) is 400, the time (seconds) is 60, the number of filter discs is 5, and the sample chamber gas is vacuum.

[0018] Furthermore, one or more of Na, Mg, Al, Si, P, and S are added in sequence to the sample tested under condition one as needed, and corresponding analysis data are obtained.

[0019] Furthermore, one or more of K, Ca, Ti, Cr, Mn, and Fe are added in sequence to the sample tested under condition 2 as needed, and corresponding analysis data are obtained.

[0020] Furthermore, the average error of the curve is less than 0.046, and is smaller than the chemical allowable error.

[0021] Furthermore, the step three also includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

[0022] Furthermore, the step 4 further comprises the following steps: Compress the samples that meet the requirements, that is, spread the samples after mixing them evenly, take samples from different positions, and compress at least 5 tablets, and then compare them with the curve corresponding to the samples to see if the results are beyond the tolerance; If the comparison result between the sample and the corresponding curve is out of tolerance, the sample should be re-mixed until the comparison result between the sample and the corresponding curve is within the tolerance; Select a sample close to the average of the five samples among the five samples that do not exceed the tolerance, and record the result.

[0023] Embodiment 2: like Figure 1-2 As shown, the present invention provides a method for detecting a calibration curve of a fluorescence analyzer, comprising the following method: Step 1: Set the detection conditions and obtain condition 1 and condition 2; Step 2: number each type of sample, and then put the numbered samples into the fluorescence analyzer for analysis under condition 1 and condition 2 respectively, to obtain multiple detection spectra for each type of sample; Step 3: Use the software of the fluorescence analyzer to calculate the intensity of A detection spectra of each type of sample, optimize the calculation results and summarize them into tables and curves; Step 4: Compare the data obtained by the fluorescence analyzer of the mixed sample with the table and the curve. If the comparison result of the sample with the table and the curve is within the error range, it is qualified, otherwise it is unqualified.

[0024]

[0025] sheet Furthermore, in the condition 1, the tube pressure (KV) is 10, the tube flow (UA) is 400, the time (seconds) is 120, the filter discs are 5, and the sample chamber gas is vacuum; In the condition 2, the tube pressure (KV) is 20, the tube flow (UA) is 600, the time (seconds) is 120, the number of filter discs is 10, and the sample chamber gas is vacuum.

[0026] Furthermore, one or more of Na, Mg, Al, Si, P, and S are added in sequence to the sample tested under condition one as needed, and corresponding analysis data are obtained.

[0027] Furthermore, one or more of K, Ca, Ti, Cr, Mn, and Fe are added in sequence to the sample tested under condition 2 as needed, and corresponding analysis data are obtained.

[0028] Furthermore, the average error of the curve is less than 0.046, and is smaller than the chemical allowable error.

[0029] Furthermore, the step three also includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

[0030] Furthermore, the step 4 further comprises the following steps: Compress the samples that meet the requirements, that is, spread the samples after mixing them evenly, take samples from different positions, and compress at least 5 tablets, and then compare them with the curve corresponding to the samples to see if the results are beyond the tolerance; If the comparison result between the sample and the corresponding curve is out of tolerance, the sample should be re-mixed until the comparison result between the sample and the corresponding curve is within the tolerance; Select a sample close to the average of the five samples among the five samples that do not exceed the tolerance, and record the result.

[0031] Embodiment 3: like Figure 1-2 As shown, the present invention provides a method for detecting a calibration curve of a fluorescence analyzer, comprising the following method: Step 1: Set the detection conditions and obtain condition 1 and condition 2; Step 2: number each type of sample, and then put the numbered samples into the fluorescence analyzer for analysis under condition 1 and condition 2 respectively, to obtain multiple detection spectra for each type of sample; Step 3: Use the software of the fluorescence analyzer to calculate the intensity of A detection spectra of each type of sample, optimize the calculation results and summarize them into tables and curves; Step 4: Compare the data obtained by the fluorescence analyzer of the mixed sample with the table and the curve. If the comparison result of the sample with the table and the curve is within the error range, it is qualified, otherwise it is unqualified.

[0032]

[0033] sheet Furthermore, in the condition 1, the tube pressure (KV) is 8, the tube flow (UA) is 300, the time (seconds) is 100, the filter discs are 2, and the sample chamber gas is vacuum; In the condition 2, the tube pressure (KV) is 18, the tube flow (UA) is 500, the time (seconds) is 100, the number of filter discs is 6, and the sample chamber gas is vacuum.

[0034] Furthermore, one or more of Na, Mg, Al, Si, P, and S are added in sequence to the sample tested under condition one as needed, and corresponding analysis data are obtained.

[0035] Furthermore, one or more of K, Ca, Ti, Cr, Mn, and Fe are added in sequence to the sample tested under condition 2 as needed, and corresponding analysis data are obtained.

[0036] Furthermore, the average error of the curve is less than 0.046, and is smaller than the chemical allowable error.

[0037] Furthermore, the step three also includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

[0038] Furthermore, the step 4 further comprises the following steps: Compress the samples that meet the requirements, that is, spread the samples after mixing them evenly, take samples from different positions, and compress at least 5 tablets, and then compare them with the curve corresponding to the samples to see if the results are beyond the tolerance; If the comparison result between the sample and the corresponding curve is out of tolerance, the sample should be re-mixed until the comparison result between the sample and the corresponding curve is within the tolerance; Select a sample close to the average of the five samples among the five samples that do not exceed the tolerance, and record the result.

[0039] The present invention is a fluorescence analyzer detection calibration curve method, which improves the detection precision and accuracy of the fluorescence analyzer and provides more reliable detection results for guiding production; simplifies the calibration curve establishment process and saves time and labor costs; has strong anti-interference ability and can adapt to different environmental conditions; and proves the effectiveness and practicality of the curve through practical application.

[0040] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for detecting a calibration curve of a fluorescence analyzer, characterized in that: The following methods are included: Step 1: Set the detection conditions and obtain condition 1 and condition 2; Step 2: number each type of sample, and then put the numbered samples into the fluorescence analyzer for analysis under condition 1 and condition 2 respectively, to obtain multiple detection spectra for each type of sample; Step 3: Use the software of the fluorescence analyzer to calculate the intensity of A detection spectra of each type of sample, optimize the calculation results and summarize them into tables and curves; Step 4: Compare the data obtained by the fluorescence analyzer of the mixed sample with the table and the curve. If the comparison result of the sample with the table and the curve is within the error range, it is qualified, otherwise it is unqualified.

2. A method for detecting a calibration curve of a fluorescence analyzer according to claim 1, characterized in that: In the condition 1, the tube pressure (KV) is 5-10, the tube flow (UA) is 100-400, the time (seconds) is 60-120, the filter is 1-5, and the sample chamber gas is vacuum; In the condition 2, the tube pressure (KV) is 15-20, the tube flow (UA) is 400-600, the time (seconds) is 60-120, the filter discs are 5-10, and the sample chamber gas is vacuum.

3. A method for detecting a calibration curve of a fluorescence analyzer according to claim 1, characterized in that: One or more of Na, Mg, Al, Si, P, and S are added to the sample tested under condition 1 in sequence as needed, and corresponding analysis data are obtained.

4. A method for detecting a calibration curve of a fluorescence analyzer according to claim 1, characterized in that: One or more of K, Ca, Ti, Cr, Mn and Fe are added in sequence to the sample tested under condition 2 as needed, and corresponding analysis data are obtained.

5. The method for detecting a calibration curve of a fluorescence analyzer according to claim 1, characterized in that: The average error of the curve is less than 0.046, and is smaller than the chemical allowable error.

6. A method for detecting a calibration curve of a fluorescence analyzer according to claim 5, characterized in that: The step three also includes the following sub-steps: If the average error exceeds 0.046 or exceeds the chemical allowable error, the sample must be re-compressed and measured; If the error is still large after the sample is re-compressed, the sample should be re-analyzed chemically; If the result of the re-chemical analysis of the sample is within the tolerance of the original chemical analysis result, but the error with the fluorescence content is large, it is necessary to add the influencing elements again.

7. A method for detecting a calibration curve of a fluorescence analyzer according to claim 1, characterized in that: The step 4 also includes the following steps: Compress the samples that meet the requirements, that is, spread the samples after mixing them evenly, take samples from different positions, and compress at least 5 tablets, and then compare them with the curve corresponding to the samples to see if the results are beyond the tolerance; If the comparison result between the sample and the corresponding curve is out of tolerance, the sample should be re-mixed until the comparison result between the sample and the corresponding curve is within the tolerance; Select a sample close to the average of the five samples among the five samples that do not exceed the tolerance, and record the result.

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