Preparation method of hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract

Abstract

The invention belongs to the technical field of hydrogel, and relates to a preparation method of a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract. According to the preparation method disclosed by the invention, a hydrogel drug loading system is formed by the hawthorn pectin and alcohol-soluble components in the hawthorn for the first time, the hawthorn pectin is gelatinized by adjusting the pH value and adding a co-solute so as to form the drug loading system, and when the pH value is relatively low, the gelatinization of the high-ester pectin is more facilitated, and the encapsulation efficiency of flavonoid components is more favorably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of hydrogels and relates to a method for preparing a hydrogel drug-carrying system based on hawthorn pectin and hawthorn alcohol extracts. Background Art

[0002] hawthorn( Crataegus pinnatida Bge.var.majorN.E.Br. or Hawthorn Crataegus pinnatifida Bge.) is a widely distributed medicinal and edible plant with the functions of promoting digestion and strengthening the spleen, promoting qi and dispersing blood stasis, clearing turbidity and reducing lipids. It is often used to treat meat stagnation, stomach distension, heart and abdomen pain, chest pain, and prevent and treat acute myocardial ischemia. Hawthorn contains a variety of chemical components, including alcohol-soluble components such as flavonoids and phenylpropionic acid. Studies have found that these components are easily oxidized and have low bioavailability. Therefore, improving the bioavailability of these components is the current research focus.

[0003] Pectin can be used to construct a colonic flora / enzyme-triggered targeted drug delivery system, and pectin has good gel properties, so it is often used as a gelling agent. Current research on pectin-based gel drug delivery is mainly focused on low-ester pectin, or on converting high-ester pectin into low-ester pectin to form a gel drug delivery. Low-ester pectin is affected by Ca 2+ The influence of divalent metal ions such as Ca can form an "egg box" structure to load drugs, but 2+ Too high or too low concentration will affect the gelation rate, gel strength, and drug release rate, thereby affecting the bioavailability of the drug, while high-ester pectin requires co-solvents such as sucrose to assist in gelation.

[0004] The degree of esterification (DE) of hawthorn pectin is greater than 50%, which belongs to high-ester pectin. The present invention uses hawthorn pectin and hawthorn alcohol extract to prepare hydrogel, which can improve the stability and bioavailability of effective ingredients such as flavonoids and phenylpropionic acid in hawthorn; in addition, hawthorn pectin has a certain effect of lowering blood lipids and protecting myocardial ischemia. The use of the two to prepare hydrogel embodies the characteristics of combining medicine and adjuvant, can retain the medicinal properties of traditional Chinese medicine to the greatest extent, is natural, has a wide source, and has good biocompatibility. Summary of the invention

[0005] In view of the above technical problems, the present invention provides a method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract. The present invention can form a gel by encapsulating these components (flavonoids, phenylpropionic acid and other alcohol-soluble components) under specific conditions, thereby improving the stability and utilization of these components.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions.

[0007] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract (hawthorn alcohol extract); Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3, dissolving the hawthorn pectin in step 2 in water to obtain solution A, and dissolving the hawthorn alcohol extract in 75% ethanol to obtain solution B; adding solution B dropwise into solution A, adjusting the pH value after adding an appropriate amount of co-solute, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzing with a 3500 Da dialysis bag to obtain a self-assembled solution, and freeze-drying the obtained solution to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0008] Furthermore, the concentration of solution B is 100 g / L ~ 80 g / L.

[0009] Furthermore, the concentration of solution A is 5 g / L to 95 g / L; the volume ratio of solution A to solution B is 2:1 to 12:1 (V A :V B ).

[0010] Furthermore, in step 3, the co-solute is glucose; and the final concentration of the co-solute in the system is 55% to 85% (w / v).

[0011] Furthermore, in step 3, the pH value is between 1.5 and 2.

[0012] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation rate of the hydrogel was calculated at the same time. The calculation formulas for the drug loading and encapsulation rate are as follows: Drug loading amount = total flavonoid mass in hydrogel / mass of pectin drug loading system × 100%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100%.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows.

[0014] Alcohol-soluble components such as flavonoids and phenylpropionic acids in hawthorn are easily affected by conditions such as temperature and humidity and are easily oxidized. The present invention proposes for the first time to form a hydrogel drug delivery system with hawthorn pectin and alcohol-soluble components in hawthorn, and to gel the hawthorn pectin by adjusting the pH value and the conditions of the co-solute to form a drug delivery system. When the pH value is low, it is more conducive to the gelation of high-ester pectin, and is more conducive to improving the encapsulation rate of flavonoid components, thereby increasing its stability and bioavailability. DETAILED DESCRIPTION

[0015] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract (hawthorn alcohol extract); Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3, dissolving the hawthorn pectin in step 2 in water to obtain solution A, and dissolving the hawthorn alcohol extract in 75% ethanol to obtain solution B; adding solution B dropwise into solution A, adjusting the pH value after adding an appropriate amount of co-solute, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzing with a 3500 Da dialysis bag to obtain a self-assembled solution, and freeze-drying the obtained solution to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0017] Furthermore, the concentration of solution B is 100 g / L to 80 g / L, preferably 95 g / L.

[0018] Furthermore, the concentration of solution A is 5 g / L to 95 g / L, preferably 35 g / L; the volume ratio of solution A to solution B is 2:1 to 12:1 (V A :V B ), preferably 2:1 (V A :V B ).

[0019] Furthermore, in step 3, the co-solute is glucose; the final concentration of the co-solute in the system is 55% to 85% (w / v), preferably 75% (w / v).

[0020] Furthermore, in step 3, the pH value is between 1.5 and 2, preferably 1.5.

[0021] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation rate of the hydrogel was calculated at the same time. The calculation formulas for the drug loading and encapsulation rate are as follows: Drug loading amount = total flavonoid mass in hydrogel / mass of pectin drug loading system × 100%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100%.

[0022] Example 1.

[0023] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B), solution B was added dropwise into solution A, 75% (w / v) co-solvent glucose was added and the pH value was adjusted to 1.5, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembly solution, and the obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0024] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading amount = total flavonoids mass in hydrogel / mass of pectin drug loading system × 100% = 18.68%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 83.52%.

[0025] Comparative example 1.

[0026] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B ), solution B was added dropwise into solution A, and the pH value was adjusted to 1.5 after adding 75% (w / v) co-solute sucrose, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembly solution. The obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0027] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading amount = total flavonoids mass in hydrogel / mass of pectin drug loading system × 100% = 12.23%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 57.43%.

[0028] Comparative example 2.

[0029] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B ), solution B was added dropwise into solution A, and the pH value was adjusted to 2.5 after adding 75% (w / v) co-solvent glucose, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembled solution. The obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0030] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading = total flavonoid mass in hydrogel / mass of pectin drug loading system × 100% = 16.57%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 81.11%.

[0031] Comparative example 3.

[0032] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B ), solution B was added dropwise into solution A, 75% (w / v) co-solvent glucose was added and the pH value was adjusted to 0.5, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembled solution, and the obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts.

[0033] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading amount = total flavonoids mass in hydrogel / mass of pectin drug loading system × 100% = 15.08%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 68.78%.

[0034] Comparative example 4.

[0035] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B ), solution B was added dropwise into solution A, 75% (w / v) of co-solvent glucose was added, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembly solution, and the obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0036] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading amount = total flavonoid mass in hydrogel / mass of pectin drug loading system × 100% = 13.59%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 74.16%.

[0037] Comparative example 5.

[0038] A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extracts, comprising the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract; Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, extract under reflux at 90°C for 3 h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3: Dissolve the hawthorn pectin in step 2 in water to obtain solution A with a concentration of 35 g / L, and dissolve the hawthorn alcohol extract in 75% ethanol to obtain solution B with a concentration of 95 g / L; A :V B ), solution B was added dropwise into solution A, the pH value was adjusted to 1.5, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzed with a 3500 Da dialysis bag to obtain a self-assembly solution, and the obtained solution was freeze-dried to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

[0039] The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation efficiency of the hydrogel was calculated at the same time. The calculation of the drug loading and encapsulation efficiency is as follows: Drug loading amount = total flavonoids mass in hydrogel / mass of pectin drug loading system × 100% = 19.05%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100% = 70.50%.

Claims

1. A method for preparing a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract, characterized in that: The method comprises the following preparation steps: Step 1, prepare hawthorn active ingredient extract: weigh hawthorn powder and put it into a conical flask, add 75% ethanol, mix evenly according to the ratio of solid to liquid 1:25 (w:v), seal it, weigh it, let it stand for 1 hour, connect a reflux condenser, heat it to boiling, and keep it at a slight boiling for 1 hour, filter it while it is hot, and concentrate it under reduced pressure until the solution no longer flows out, so as to obtain the extract (hawthorn alcohol extract); Step 2, preparing hawthorn pectin: evenly mix hawthorn powder and water in a solid-liquid ratio of 1:20, reflux extract at 90°C for 3h, filter, concentrate the filtrate to half of the original volume, cool and add 2 times the volume of 95% ethanol, precipitate overnight, collect the precipitate; elute with anhydrous ethanol, collect the precipitate to obtain wet pectin, freeze-dry to obtain hawthorn pectin, and store in a 4°C refrigerator for later use; Step 3, dissolving the hawthorn pectin in step 2 in water to obtain solution A, and dissolving the hawthorn alcohol extract in 75% ethanol to obtain solution B; adding solution B dropwise into solution A, adjusting the pH value after adding an appropriate amount of co-solute, followed by magnetic stirring, ultrasonic oscillation, standing at room temperature, and dialyzing with a 3500 Da dialysis bag to obtain a self-assembled solution, and freeze-drying the obtained solution to obtain a hydrogel drug delivery system based on hawthorn pectin and hawthorn alcohol extract.

2. The method for preparing the hydrogel drug delivery system according to claim 1, characterized in that: The concentration of solution B is 100g / L~80g / L.

3. The method for preparing the hydrogel drug delivery system according to claim 1, characterized in that: The concentration of solution A is 5g / L~95g / L; the volume ratio of solution A to solution B is 2:1~12:

1.

4. The method for preparing the hydrogel drug delivery system according to claim 1, characterized in that: In step 3, the cosolute is glucose; the final concentration of the cosolute in the system is 55%~85% (w / v).

5. The method for preparing the hydrogel drug delivery system according to claim 1, characterized in that: In step 3, the pH value is between 1.5 and 2.

6. The method for preparing the hydrogel drug delivery system according to any one of claims 1 to 5, characterized in that: The total flavonoids in the hydrogel of hawthorn pectin and hawthorn alcohol extracts were determined spectrophotometrically using the sodium nitrite-aluminum nitrate method. The drug loading of the hydrogel was used as the main criterion, and the encapsulation rate of the hydrogel was calculated at the same time. The calculation formulas for the drug loading and encapsulation rate are as follows: Drug loading amount = total flavonoid mass in hydrogel / mass of pectin drug loading system × 100%; Encapsulation efficiency = total flavonoid mass in hydrogel / total flavonoid mass of drug input during preparation × 100%.