Method for anaerobically degrading cellulose

Abstract

The invention discloses a method for anaerobically degrading cellulose, which comprises the following steps: preparing a biological culture medium by taking agricultural and forestry wastes as main materials and starch raw materials as auxiliary materials; the method comprises the following steps: inoculating a phanerochaete chrysosporium spore suspension into a biological culture medium, and performing mycelium culture twice to obtain a phanerochaete chrysosporium mycelium culture product; the phanerochaete chrysosporium mycelium culture product is dried and shaped, and the phanerochaete chrysosporium mycelium-based composite material is obtained; the method comprises the following steps: pretreating cellulose kitchen wastes by using a phanerochaete chrysosporium mycelium-based composite material, mixing the pretreated cellulose kitchen wastes with inoculated sludge, adjusting the water content to 90%, and carrying out standing anaerobic fermentation in a reaction container. The method has a good application prospect in anaerobic fermentation of agricultural and forestry wastes, urban organic solid wastes and the like with relatively high cellulose content.

Description

Technical Field

[0001] The invention belongs to the field of bio-based material technology and resource processing and disposal of organic solid waste, and specifically relates to a method for anaerobic degradation of cellulose. Background Art

[0003] As a mature organic solid waste treatment technology, anaerobic digestion has good environmental benefits and is considered to be one of the most feasible methods for managing organic waste. During the anaerobic digestion process, organic matter is consumed by microorganisms and converted into energy substances. This process includes four stages: hydrolysis, acidification, hydrogen and acetic acid production, and methanogenesis. Since agricultural waste and urban organic solid waste contain a large amount of macromolecular substances such as lignin, cellulose, and hemicellulose, hydrolysis becomes the rate-limiting step in the anaerobic fermentation of solid waste. For the research on the difficult hydrolysis of cellulose, the focus was previously on pretreatment with chemical, physical, and physicochemical processes such as acid, alkaline, freeze-thaw, high temperature, and ultrasound to improve its hydrolysis performance, and its huge cost, energy consumption, and secondary pollution problems were also introduced. Therefore, how to improve the method of hydrolysis of cellulose biomass is a major problem to improve the process efficiency and economic feasibility of anaerobic digestion of organic solid waste.

[0004] Bio-augmentation using cellulose-degradable microorganisms is a method for improving the efficiency of anaerobic digestion by hydrolysis. Mycelium-based composite materials have received widespread attention since they were proposed in 2007. They form a tight network structure by combining microorganisms with agricultural and forestry wastes, and can replace many high-energy-consuming materials. This type of material is easy to obtain, degradable, renewable, and has strong plasticity. It is currently widely used in many fields. The present invention loads cellulose-degrading bacteria onto bio-based composite materials to prepare anaerobic mycelium materials. After being added to the anaerobic fermentation system, it can effectively promote the hydrolysis process of lignin and cellulose wastes, promote energy generation, and achieve the effect of treating waste with waste.

[0005] Compared with the existing technology, the currently cultivated mycelium materials are mainly used in the aerobic field, and there is little research in the anaerobic field. Therefore, it is necessary to develop mycelium materials in the anaerobic field to meet future market demand. Summary of the invention

[0006] The purpose of the present invention is to provide a method for anaerobic degradation of cellulose. Specifically, a mycelium material capable of anaerobically degrading cellulose is prepared and applied to the field of anaerobic fermentation of cellulose waste to promote the hydrolysis process of cellulose-based difficult-to-degrade organic matter and improve energy efficiency.

[0007] To achieve the above object, the present invention adopts the following technical solution: A method for anaerobic cellulose degradation comprises the following steps: S1: Prepare biological culture medium with agricultural and forestry waste as main material and starch raw materials as auxiliary material; S2: inoculating the spore suspension of Phanerochaete chrysosporium into a biological culture medium, and performing two mycelium cultures to obtain a mycelium culture product of Phanerochaete chrysosporium; S3: drying and shaping the culture product of Phanerochaete chrysosporium mycelium to obtain a Phanerochaete chrysosporium mycelium-based composite material; S4: The cellulosic kitchen waste is pretreated with a mycelium-based composite material of Phanerochaete chrysosporium, and then mixed with the inoculated sludge, the moisture content is adjusted to 90%, and static anaerobic fermentation is carried out in a reaction container.

[0008] In step S1, the agricultural and forestry waste is any one or more of rice husk, rice straw, poplar wood chips, and corn stalks, and the starchy raw material is any one or more of wheat flour, wheat bran, and corn starch.

[0009] In step S1, the weight ratio of the main material to the auxiliary material is 80-90:10-20.

[0010] In step S2, the method of culturing the mycelium twice is as follows: inoculating the spore suspension of Phanerochaete chrysosporium into a biological culture medium, culturing the culture medium under the conditions of temperature 25±2°C, humidity 60±5%, no light, and oxygen content 15% until the mycelium covers the culture medium, thereby obtaining a first mycelium culture product; crushing the first mycelium culture product until the mycelium is invisible to the naked eye, adding wheat flour and nutrient solution to mix, and culturing the culture medium under the conditions of temperature 25±2°C, humidity 60±5%, no light, and oxygen content 15% for 10 days to obtain a second mycelium culture product, i.e., a Phanerochaete chrysosporium mycelium culture product; the ratio of the first mycelium culture product: wheat flour: nutrient solution is 80g:20g:10ml; the formula of the nutrient solution is: NaNO 3 2g / L, K 2 HPO 4 1g / L, MgSO 4 0.5g / L, KCl 0.5g / L, peptone 2g / L, and the rest are sterile water.

[0011] In step S3, the drying conditions are: 60° C., 1.5 hours.

[0012] In step S4, the weight ratio of the cellulose kitchen waste to the mycelium-based composite material is 100:10, and the volatile solid ratio of the cellulose kitchen waste to the inoculated sludge is 1:2.

[0013] In step S4, the pretreatment time is 72 hours.

[0014] In step S4, the temperature of the anaerobic fermentation is 37°C.

[0015] The significant advantages of the present invention are: The method of the present invention is applied to the anaerobic fermentation of cellulose-containing food waste, has good cellulose hydrolysis function, and promotes the production of methane; compared with the control group, the cellulose degradation rate before and after anaerobic fermentation is increased by 10% to 14%, and the methane yield is greatly increased. Therefore, the present invention provides a method for improving the efficiency of anaerobic biodegradation of cellulose, and has good application prospects in the anaerobic fermentation of agricultural and forestry wastes with high cellulose content, urban organic solid wastes, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a photo of the hyphae of Phanerochaete chrysosporium covering the biological culture medium.

[0017] Figure 2 This is a scanning electron microscope image of celery after anaerobic fermentation in Example 2.

[0018] Figure 3 This is a scanning electron microscope image of the surface of celery after anaerobic fermentation in Comparative Example 2.

[0019] Figure 4 It is a graph showing the cumulative methane production in the gas produced by anaerobic fermentation in each embodiment and comparative example.

[0020] Figure 5 Graph showing the methane content in the gas produced by anaerobic fermentation in each embodiment and comparative example. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0022] The Phanerochaete chrysosporium involved in the following examples was purchased from Beina Biotech-Henan Industrial Microbial Strain Engineering Technology Research Center, with the number BNCC336257.

[0023] The PDA solid culture medium involved in the following embodiments is: take 200g of peeled potatoes, add 1000ml of water, boil for 30min, filter with gauze, then weigh 10g of glucose and 15g of agar powder and add them to the filtrate, dilute to 1L with distilled water, and sterilize at 121°C for 20 minutes.

[0024] The formula of the nutrient solution involved in the following embodiments is: NaNO 3 2g / L, K 2 HPO 4 1g / L, MgSO 40.5g / L, KCl0.5g / L, peptone 2g / L, and the rest is distilled water.

[0025] The inoculated sludge involved in the following examples comes from a thickening tank of a sewage treatment plant in Fuzhou City, and its initial characteristics are as follows: C / N is 7.05, VS / TS (%) is 0.36±0.04, and pH is 7.02±0.01.

[0026] Embodiment 1: This embodiment provides a method for anaerobic degradation of cellulose, which is carried out according to the following steps: S1: Biological culture medium preparation: Rice husks, rice straw, poplar wood chips and corn stalks are naturally air-dried, crushed to a particle size range of 3-5 mm and an average particle size of 3.9 mm, and then mixed at a weight ratio of 20:35:15:30 as the main ingredient; wheat flour is used as an auxiliary material; the main ingredient and the auxiliary material are mixed at a weight ratio of 80:20, distilled water is added to adjust the moisture content to 60%, put into a sterilization bag, and sterilize at 121°C for 20 minutes to obtain a biological culture medium.

[0027] S2: Preparation of composite materials based on mycelium of Phanerochaete chrysosporium: S2-1: The strain of Phanerochaete chrysosporium stored at 4°C was inoculated into a PDA solid culture medium for activation for 3 generations to obtain activated Phanerochaete chrysosporium; the activated Phanerochaete chrysosporium was added to sterile water, the surface of the agar was scraped with an inoculation loop to scrape off the spores, the spore suspension was collected, shaken, filtered with sterile filter paper, counted, and sterile water was added again to adjust the OD 600 The value is 1.0, and the spore suspension of Phanerochaete chrysosporium is obtained; S2-2: Inoculate the spore suspension of Phanerochaete chrysosporium into the biological culture medium at an inoculum ratio of 10:100 (ml:g), and culture at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% until the mycelium covers the culture medium, thereby obtaining the first mycelium culture product; S2-3: The first mycelium culture product was crushed until the mycelium could not be seen by naked eyes, and wheat flour and nutrient solution were added and mixed, the ratio of the first mycelium culture product: wheat flour: nutrient solution was 80:20:10 (g:g:ml), and cultured for 10 days at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% to obtain a second mycelium culture product; S2-4: drying the second mycelium culture product at 60° C. for 1.5 hours to fix the product, thereby obtaining a mycelium-based composite material of Phanerochaete chrysosporium.

[0028] S3: Anaerobic degradation of cellulose: S3-1: Wash and dry freshly purchased celery, crush it with a grinder, and then dry it at 165°C for 24 hours. After drying, crush it with a grinder and pass it through a 40-mesh sieve to obtain cellulose kitchen waste; S3-2: 500 g of cellulose kitchen waste was taken, sterile water was added to adjust the moisture content to 70%, and then soaked for 12 hours, and then 60 g of the mycelium-based composite material of Phanerochaete chrysosporium was added, and the mixture was fully stirred and covered with a plastic film for 72 hours to obtain pretreated cellulose kitchen waste; S3-3: Mix the pretreated cellulosic kitchen waste and inoculated sludge in a ratio of 1:2 (in terms of VS), adjust the moisture content to 90% with sterile water, weigh 600 g and place it in a 1L borosilicate biological feeding bottle, fill the bottle with high-purity nitrogen for 10 minutes to drive out all the air in the bottle, quickly seal it with a rubber stopper, and place it in an incubator at a constant temperature of 37°C for static anaerobic fermentation for 20 days.

[0029] The total cellulose content in the fermentation system was measured before and after static anaerobic fermentation, and gas was collected daily during the fermentation to test the methane concentration and volume.

[0030] Embodiment 2: This embodiment provides a method for anaerobic degradation of cellulose, which is carried out according to the following steps: S1: Biological culture medium preparation: The rice husk and poplar wood chips are naturally air-dried, crushed to a particle size range of 3-5 mm and an average particle size of 3.9 mm, and then mixed at a weight ratio of 80:10 to serve as the main ingredient; the wheat bran is crushed to a particle size range of 40-60 μm and an average particle size of 51 μm, and then mixed with corn starch at a weight ratio of 1:1 to serve as the auxiliary material; the main ingredient and the auxiliary material are mixed at a weight ratio of 90:10, distilled water is added to adjust the water content to 60%, the mixture is put into a sterilization bag, and sterilized at 121° C. for 20 minutes to obtain a biological culture medium.

[0031] S2: Preparation of composite materials based on mycelium of Phanerochaete chrysosporium: S2-1: The strain of Phanerochaete chrysosporium stored at 4°C was inoculated into a PDA solid culture medium for activation for 3 generations to obtain activated Phanerochaete chrysosporium; the activated Phanerochaete chrysosporium was added to sterile water, the surface of the agar was scraped with an inoculation loop to scrape off the spores, the spore suspension was collected, shaken, filtered with sterile filter paper, counted, and sterile water was added again to adjust the OD 600 The value is 1.0, and the spore suspension of Phanerochaete chrysosporium is obtained; S2-2: Inoculate the spore suspension of Phanerochaete chrysosporium into the biological culture medium at an inoculum ratio of 10:100 (ml:g), and culture at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% until the mycelium covers the culture medium, thereby obtaining the first mycelium culture product; S2-3: The first mycelium culture product was crushed until the mycelium could not be seen by naked eyes, and wheat flour and nutrient solution were added and mixed, the ratio of the first mycelium culture product: wheat flour: nutrient solution was 80:20:10 (g:g:ml), and cultured for 10 days at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% to obtain a second mycelium culture product; S2-4: drying the second mycelium culture product at 60° C. for 1.5 hours to fix the product, thereby obtaining a mycelium-based composite material of Phanerochaete chrysosporium.

[0032] S3: Anaerobic degradation of cellulose: S3-1: Wash and dry freshly purchased celery, crush it with a grinder, and then dry it at 165°C for 24 hours. After drying, crush it with a grinder and pass it through a 40-mesh sieve to obtain cellulose kitchen waste; S3-2: 500 g of cellulose kitchen waste was taken, sterile water was added to adjust the moisture content to 70%, and then soaked for 12 hours, and then 60 g of the mycelium-based composite material of Phanerochaete chrysosporium was added, and the mixture was fully stirred and covered with a plastic film for 72 hours to obtain pretreated cellulose kitchen waste; S3-3: Mix the pretreated cellulosic kitchen waste and inoculated sludge in a ratio of 1:2 (in terms of VS), adjust the moisture content to 90% with sterile water, weigh 600 g and place it in a 1L borosilicate biological feeding bottle, fill the bottle with high-purity nitrogen for 10 minutes to drive out all the air in the bottle, quickly seal it with a rubber stopper, and place it in an incubator at a constant temperature of 37°C for static anaerobic fermentation for 20 days.

[0033] The total cellulose content in the fermentation system was measured before and after static anaerobic fermentation, and gas was collected daily during the fermentation to test the methane concentration and volume.

[0034] Embodiment 3: This embodiment provides a method for anaerobic degradation of cellulose, which is carried out according to the following steps: S1: Biological culture medium preparation: Rice husks, rice straw, poplar wood chips and corn stalks are naturally air-dried, crushed to a particle size range of 3-5 mm and an average particle size of 3.9 mm, and then mixed at a weight ratio of 20:35:15:30 to serve as the main ingredient; wheat bran is crushed to a particle size range of 40-60 μm and an average particle size of 51 μm, and then mixed with wheat flour at a weight ratio of 1:1 to serve as the auxiliary material; the main ingredient and the auxiliary material are mixed at a weight ratio of 80:20, distilled water is added to adjust the water content to 60%, the mixture is put into a sterilization bag, and sterilized at 121° C. for 20 minutes to obtain a biological culture medium.

[0035] S2: Preparation of composite materials based on mycelium of Phanerochaete chrysosporium: S2-1: The strain of Phanerochaete chrysosporium stored at 4°C was inoculated into a PDA solid culture medium for activation for 3 generations to obtain activated Phanerochaete chrysosporium; the activated Phanerochaete chrysosporium was added to sterile water, the surface of the agar was scraped with an inoculation loop to scrape off the spores, the spore suspension was collected, shaken, filtered with sterile filter paper, counted, and sterile water was added again to adjust the OD 600 The value is 1.0, and the spore suspension of Phanerochaete chrysosporium is obtained; S2-2: Inoculate the spore suspension of Phanerochaete chrysosporium into the biological culture medium at an inoculum ratio of 10:100 (ml:g), and culture at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% until the mycelium covers the culture medium, thereby obtaining the first mycelium culture product; S2-3: The first mycelium culture product was crushed until the mycelium could not be seen by naked eyes, and wheat flour and nutrient solution were added and mixed, the ratio of the first mycelium culture product: wheat flour: nutrient solution was 80:20:10 (g:g:ml), and cultured for 10 days at a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% to obtain a second mycelium culture product; S2-4: drying the second mycelium culture product at 60° C. for 1.5 hours to fix the product, thereby obtaining a mycelium-based composite material of Phanerochaete chrysosporium.

[0036] S3: Anaerobic degradation of cellulose: S3-1: Wash and dry freshly purchased celery, crush it with a grinder, and then dry it at 165°C for 24 hours. After drying, crush it with a grinder and pass it through a 40-mesh sieve to obtain cellulose kitchen waste; S3-2: 500 g of cellulose kitchen waste was taken, sterile water was added to adjust the moisture content to 70%, and then soaked for 12 hours, and then 60 g of the mycelium-based composite material of Phanerochaete chrysosporium was added, and the mixture was fully stirred and covered with a plastic film for 72 hours to obtain pretreated cellulose kitchen waste; S3-3: Mix the pretreated cellulosic kitchen waste and inoculated sludge in a ratio of 1:2 (in terms of VS), adjust the moisture content to 90% with sterile water, weigh 600 g and place it in a 1L borosilicate biological feeding bottle, fill the bottle with high-purity nitrogen for 10 minutes to drive out all the air in the bottle, quickly seal it with a rubber stopper, and place it in an incubator at a constant temperature of 37°C for static anaerobic fermentation for 20 days.

[0037] The total cellulose content in the fermentation system was measured before and after static anaerobic fermentation, and gas was collected daily during the fermentation to test the methane concentration and volume.

[0038] Comparative Example 1: This embodiment provides a method for anaerobic degradation of cellulose, which is carried out according to the following steps: S1: Preparation of Phanerochaete chrysosporium spore suspension: S1-1: Inoculate the strain of Phanerochaete chrysosporium stored at 4°C into PDA solid culture medium for activation for 3 generations to obtain activated Phanerochaete chrysosporium; add sterile water to the activated Phanerochaete chrysosporium, scrape the surface of agar with an inoculation loop to scrape off the spores, collect the spore suspension, shake, filter with sterile filter paper, count, add sterile water again and adjust its OD 600 The value is 1.0, and a spore suspension of Phanerochaete chrysosporium is obtained.

[0039] S2: Anaerobic degradation of cellulose: S2-1: Wash and dry freshly purchased celery, crush it with a grinder, and then dry it at 165°C for 24 hours. After drying, crush it with a grinder and pass it through a 40-mesh sieve to obtain cellulose kitchen waste; S2-2: 500 g of cellulosic kitchen waste was taken, sterile water was added to adjust the moisture content to 70%, and then soaked for 12 hours, and then a spore suspension of Phanerochaete chrysosporium was added at an inoculum amount of 5% (v / v), and the mixture was fully stirred and covered with a plastic film for 72 hours to obtain pretreated cellulosic kitchen waste; S2-3: Mix the pretreated cellulose kitchen waste and inoculated sludge in a ratio of 1:2 (in terms of VS), adjust the moisture content to 90% with sterile water, weigh 600 g and place it in a 1L borosilicate biological feeding bottle, fill the bottle with high-purity nitrogen for 10 minutes to drive out all the air in the bottle, quickly seal it with a rubber stopper, and place it in an incubator at a constant temperature of 37°C for static anaerobic fermentation for 20 days.

[0040] The total cellulose content in the fermentation system was measured before and after static anaerobic fermentation, and gas was collected daily during the fermentation to test the methane concentration and volume.

[0041] Comparative Example 2: This embodiment provides a method for anaerobic degradation of cellulose, which is carried out according to the following steps: S1: Wash and dry the freshly purchased celery, crush it with a grinder, and then dry it at 165°C for 24 hours. After drying, crush it with a grinder and pass it through a 40-mesh sieve to obtain cellulose kitchen waste; S2: Take 500 g of cellulose kitchen waste, add sterile water to adjust the moisture content to 70%, soak for 12 hours, stir well and cover with plastic film for 72 hours to obtain pretreated cellulose kitchen waste; S3-3: Mix the pretreated cellulosic kitchen waste and inoculated sludge in a ratio of 1:2 (in terms of VS), adjust the moisture content to 90% with sterile water, weigh 600 g and place it in a 1L borosilicate biological feeding bottle, fill the bottle with high-purity nitrogen for 10 minutes to drive out all the air in the bottle, quickly seal it with a rubber stopper, and place it in an incubator at a constant temperature of 37°C for static anaerobic fermentation for 20 days.

[0042] The total cellulose content in the fermentation system was measured before and after static anaerobic fermentation, and gas was collected daily during the fermentation to test the methane concentration and volume.

[0043] Figure 1 This is a photo of the hyphae of Phanerochaete chrysosporium covering the biological culture medium. The surface morphology of celery before and after anaerobic fermentation was observed by scanning electron microscopy. Figure 2 This is a scanning electron microscope image of celery after anaerobic fermentation in Example 2. Figure 3 This is a scanning electron microscope image of the surface of celery after anaerobic fermentation in Comparative Example 2. Figure 4 It is a graph showing the cumulative methane production in the gas produced by anaerobic fermentation in each embodiment and comparative example. Figure 5 Table 1 shows the results of measuring the total cellulose content in the fermentation system before and after anaerobic fermentation.

[0044] Table 1 Total cellulose content before and after anaerobic fermentation (unit: %) The test found that pretreatment with spore suspension of Phanerochaete chrysosporium can promote rapid fermentation and methane production. Since the activity of hydrolytic bacteria is strong in the early stage and the activity of methanogens is weak, acidification is easy to occur, thereby inhibiting methane production. After adding the mycelium-based composite material of Phanerochaete chrysosporium, the fungi that can degrade cellulose can be well revived in an anaerobic environment, produce cellulose hydrolases, and the action lasts for a long time, which has a certain promoting effect on the degradation of cellulose in the fermentation system. The scanning electron microscope (SEM) image shows that the mycelium-based composite material of Phanerochaete chrysosporium promotes the structural destruction of cellulose to produce fragments, thereby improving its hydrolysis efficiency, while the fiber surface of the group not treated with Phanerochaete chrysosporium is smooth and has no grooves. The cumulative methane production graph and the methane content graph show that the addition of the mycelium-based composite material of Phanerochaete chrysosporium can construct a co-culture system of white rot fungi and methanogens, which greatly increases the methane production. The methane content is high in the early stage of pretreatment with spore suspension of Phanerochaete chrysosporium, and inhibition occurs in the later stage, and the methane content decreases.

[0045] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A method for anaerobic degradation of cellulose, characterized in that: The steps include: S1: Prepare biological culture medium with agricultural and forestry waste as main material and starch raw materials as auxiliary material; S2: inoculating the spore suspension of Phanerochaete chrysosporium into a biological culture medium, and performing two mycelium cultures to obtain a mycelium culture product of Phanerochaete chrysosporium; S3: drying and shaping the culture product of Phanerochaete chrysosporium mycelium to obtain a Phanerochaete chrysosporium mycelium-based composite material; S4: The cellulosic kitchen waste is pretreated with a mycelium-based composite material of Phanerochaete chrysosporium, and then mixed with the inoculated sludge, the moisture content is adjusted to 90%, and static anaerobic fermentation is carried out in a reaction container.

2. The method according to claim 1, characterized in that: The agricultural and forestry waste is any one or more of rice husks, rice straw, poplar wood chips, and corn stalks, and the starchy raw material is any one or more of wheat flour, wheat bran, and corn starch.

3. The method according to claim 1, characterized in that: The weight ratio of the main material to the auxiliary material is 80-90:10-20.

4. The method according to claim 1, characterized in that: The method for culturing the mycelium twice is as follows: inoculating a spore suspension of Phanerochaete chrysosporium into a biological culture medium, culturing the culture medium under the conditions of a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15% until the mycelium covers the culture medium, thereby obtaining a first mycelium culture product; crushing the first mycelium culture product until the mycelium is invisible to the naked eye, adding wheat flour and a nutrient solution to mix, and culturing the culture medium for 10 days under the conditions of a temperature of 25±2°C, a humidity of 60±5%, no light, and an oxygen content of 15%, thereby obtaining a second mycelium culture product, namely, a mycelium culture product of Phanerochaete chrysosporium; the ratio of the first mycelium culture product: wheat flour: nutrient solution is 80g:20g:10ml; the formula of the nutrient solution is: NaNO3 2g / L, K2HPO4 1g / L, MgSO4 0.5g / L, KCl 0.5g / L, peptone 2g / L, and the rest is sterile water.

5. The method according to claim 1, characterized in that: The drying conditions are: 60° C., 1.5 hours.

6. The method according to claim 1, characterized in that: The weight ratio of the cellulose kitchen waste to the mycelium-based composite material of Phanerochaete chrysosporium is 100:10, and the volatile solid ratio of the cellulose kitchen waste to the inoculated sludge is 1:

2.

7. The method according to claim 1, characterized in that: The pretreatment time is 72h.

8. The method according to claim 1, characterized in that: The temperature of the anaerobic fermentation is 37°C.

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