Method and device for testing sterilization capability of sterilization equipment and test equipment

Abstract

The invention relates to the technical field of intelligent household appliances, and discloses a method for testing the sterilization capability of sterilization equipment, and the method comprises the following steps: obtaining the first bacterial content of a sterilization bacterial liquid arranged in the sterilization equipment in a sterilization test, and the second bacterial content of a control bacterial liquid with the same storage environment as the sterilization bacterial liquid in the sterilization test; determining an initial sterilization rate of the sterilization equipment according to the first bacterium content and the second bacterium content; obtaining a test environment coefficient of the degerming equipment; and correcting the initial sterilization rate by using the test environment coefficient to obtain a test sterilization rate of the sterilization equipment, and evaluating the sterilization capability of the sterilization equipment according to the test sterilization rate. Therefore, the evaluation of the sterilization capability of the closed space such as the refrigerator can be accurately realized. The invention also discloses a device for testing the sterilization capability of the sterilization equipment and test equipment.

Description

Technical Field

[0001] The present application relates to the field of smart home appliance technology, for example, to a method, device and testing equipment for testing the sterilization ability of a sterilization device. Background Art

[0002] At present, in recent years, food safety and the sterilization function in refrigerators have become the focus of public attention. Among them, the microorganisms produced by the corruption of food ingredients during storage will pollute the refrigerator environment. Newly put food is more likely to spoil when stored in an environment contaminated by microorganisms. The microorganisms and odors produced will affect the health of consumers and there are hidden dangers to food safety.

[0003] Currently, there is only a method to test the purification and sterilization rate of air purifiers, which tests the air purification effect in non-confined spaces before and after sterilization. There is currently no corresponding method to evaluate the sterilization effect in confined spaces such as refrigerators.

[0004] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present application, and therefore may include information that does not constitute the prior art known to ordinary technicians in the field. Summary of the invention

[0005] In order to provide a basic understanding of some aspects of the disclosed embodiments, a brief summary is given below. The summary is not an extensive review, nor is it intended to identify key / critical components or delineate the scope of protection of these embodiments, but rather serves as a prelude to the detailed description that follows.

[0006] The embodiments of the present disclosure provide a method, a device and a testing device for testing the sterilization ability of a sterilization device, so as to propose a testing and evaluation method for the sterilization effect applied to a confined space such as a refrigerator.

[0007] In some embodiments, a method for testing the sterilization ability of a sterilization device is provided, comprising:

[0008] Obtaining a first bacterial content of a sterilization test of a sterilization liquid disposed in a sterilization device, and a second bacterial content of a control liquid stored in the same environment as the sterilization liquid in a sterilization test;

[0009] determining an initial sterilization rate of the sterilization device according to the first bacteria content and the second bacteria content;

[0010] Obtain the test environment coefficient of the sterilization equipment;

[0011] The initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, so as to evaluate the sterilization ability of the sterilization equipment based on the test sterilization rate.

[0012] In some embodiments of the confined space, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, including calculating the test sterilization rate as follows:

[0013] k=f×k 0

[0014] Among them, k is the test sterilization rate, k 0 is the initial sterilization rate, and f is the test environment coefficient.

[0015] In some embodiments, the test environment coefficient is calculated as follows:

[0016] f=f1, or, f=f2, or, f=f1×f2

[0017] Among them, f is the test environment coefficient, f1 is the time coefficient, and f2 is the space coefficient.

[0018] In some implementations, the time factor is calculated as follows:

[0019] f1=a / (T2-T1)

[0020] Among them, f1 is the time coefficient, a is the first adjustment parameter, T2 is the time when the bacterial content is obtained in the sterilization test, and T1 is the preset time in the sterilization test.

[0021] In some embodiments, the spatial coefficient is calculated as follows:

[0022] f2=b / V

[0023] Among them, f2 is the space coefficient, b is the second adjustment parameter, and V is the test volume of the sterilization equipment.

[0024] In some embodiments, evaluating the sterilization ability of a sterilization device according to a test sterilization rate includes:

[0025] When the test sterilization rate is greater than or equal to the set sterilization rate, it is determined that the sterilization equipment has the sterilization capability;

[0026] When the tested sterilization rate is lower than the set sterilization rate, it is determined that the sterilization equipment does not have the sterilization capability.

[0027] In some embodiments, evaluating the sterilization ability of a sterilization device according to a test sterilization rate includes:

[0028] Obtaining a first colony area parameter of a sterilization test of a sterilization liquid set in a sterilization device, and a second colony area parameter of a control bacterial liquid stored in the same environment as the sterilization liquid in a sterilization test;

[0029] Determining a colony area parameter ratio of the first colony area parameter and the second colony area parameter;

[0030] Obtain evaluation parameters for bactericidal test experiments;

[0031] The sterilization parameters of the sterilization equipment are determined based on the ratio of the evaluation parameters to the colony area parameters, so as to evaluate the sterilization ability of the sterilization equipment based on the test sterilization rate and sterilization parameters.

[0032] In some embodiments, the bactericidal solution and / or the control solution are prepared as follows:

[0033] Performing rejuvenation culture on the pasteurized milk to obtain a bacterial solution with a set colony count; wherein the rejuvenation culture includes shaking culture for a first time period, or static culture for a second time period;

[0034] The bacterial solution with a set colony count is used as a sterilization bacterial solution; and / or the bacterial solution with a set colony count is used as a control bacterial solution.

[0035] In these embodiments, the pasteurized milk is used to prepare the sterilized bacterial solution and the control bacterial solution. The bacterial colony is easy to obtain, which reduces the disadvantages of the difficulty in obtaining professional strains for microbiological operations, high costs, and strict requirements for storage equipment.

[0036] An embodiment of the present disclosure also provides a device for testing the sterilization ability of a sterilization device, comprising a processor and a memory storing program instructions, wherein the processor is configured to execute a method for testing the sterilization ability of a sterilization device provided in an embodiment of the present disclosure when executing the program instructions.

[0037] In some embodiments, a testing device is provided, comprising:

[0038] Equipment body;

[0039] The disclosed embodiment provides a device for testing the sterilization capability of a sterilization device, which is arranged on the device body.

[0040] The method, device and testing equipment for testing the sterilization ability of sterilization equipment provided by the embodiments of the present disclosure can achieve the following technical effects:

[0041] The initial sterilization rate of the sterilization equipment is determined according to the first bacterial content of the sterilization liquid in the sterilization test and the second bacterial content of the control liquid in the same storage environment as the sterilization liquid in the sterilization test, and the initial sterilization rate is corrected by the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, so as to evaluate the sterilization ability of the sterilization equipment according to the test sterilization rate. A method for evaluating the sterilization ability of a confined space such as a refrigerator is proposed. And because the initial sterilization rate of the confined space is corrected by the test environment coefficient, the sterilization ability of a confined space such as a refrigerator can be evaluated more accurately.

[0042] The above general description and the following description are exemplary and explanatory only and are not intended to limit the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] One or more embodiments are exemplarily described by corresponding drawings, which do not limit the embodiments. Elements with the same reference numerals in the drawings are shown as similar elements, and the drawings do not constitute a scale limitation, and wherein:

[0044] Figure 1 It is a flow chart of a method for testing the sterilization ability of a sterilization device provided by an embodiment of the present disclosure;

[0045] Figure 2 It is a schematic diagram of a process for evaluating the sterilization ability of a sterilization device according to a test sterilization rate provided by an embodiment of the present disclosure;

[0046] Figure 3 It is a schematic diagram of a process for preparing a bactericidal solution and / or a control solution provided in an embodiment of the present disclosure;

[0047] Figure 4 is a flow chart of another method for testing the sterilization capability of a sterilization device according to an embodiment of the present disclosure;

[0048] Figure 5 is a flow chart of a method for evaluating the sterilization ability of a sterilization device through sterilization parameters according to an embodiment of the present disclosure;

[0049] Figure 6 It is a schematic diagram of a device for testing the sterilization ability of a sterilization device provided in an embodiment of the present disclosure. DETAILED DESCRIPTION

[0050] In order to be able to understand the features and technical contents of the embodiments of the present disclosure in more detail, the implementation of the embodiments of the present disclosure is described in detail below in conjunction with the accompanying drawings. The attached drawings are for reference only and are not used to limit the embodiments of the present disclosure. In the following technical description, for the convenience of explanation, a full understanding of the disclosed embodiments is provided through multiple details. However, one or more embodiments can still be implemented without these details. In other cases, to simplify the drawings, well-known structures and devices can be simplified for display.

[0051] The terms "first", "second", etc. in the specification and claims of the embodiments of the present disclosure and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the terms used in this way can be interchanged where appropriate, so that the embodiments of the embodiments of the present disclosure described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions.

[0052] Unless otherwise stated, the term "plurality" means two or more.

[0053] In the embodiment of the present disclosure, the character " / " indicates that the preceding and following objects are in an "or" relationship. For example, A / B indicates: A or B.

[0054] The term "and / or" is a description of the association relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A or B, or, A and B.

[0055] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other.

[0056] The embodiments of the present disclosure provide a method, a device and a testing device for testing the sterilization ability of a sterilization device, so as to solve the technical problem that there is currently no testing and evaluation method for the sterilization effect of a space of household appliances such as refrigerators.

[0057] An embodiment of the present disclosure provides a device for testing the sterilization ability of a sterilization device, comprising a processor and a memory storing program instructions, wherein the processor is configured to execute a method for testing the sterilization ability of a sterilization device provided in the embodiment of the present disclosure when executing the program instructions.

[0058] In some embodiments, in combination Figure 1 As shown, a method for testing the sterilization ability of a sterilization device is provided, comprising:

[0059] S101. The processor obtains a first bacterial content of a sterilization liquid set in a sterilization device in a sterilization test, and a second bacterial content of a control liquid in a sterilization test with a storage environment being the same as that of the sterilization liquid.

[0060] S102: The processor determines an initial sterilization rate of the sterilization equipment according to the first bacteria content and the second bacteria content.

[0061] S103. The processor obtains the test environment coefficient of the sterilization equipment.

[0062] S104. The processor uses the test environment coefficient to correct the initial sterilization rate to obtain the test sterilization rate of the sterilization equipment, so as to evaluate the sterilization ability of the sterilization equipment according to the test sterilization rate.

[0063] In these embodiments, the initial sterilization rate of the sterilization device is determined according to the first bacterial content of the sterilization liquid in the sterilization test and the second bacterial content of the control liquid in the same storage environment as the sterilization liquid in the sterilization test. The initial sterilization rate is corrected by the test environment coefficient to obtain the test sterilization rate of the sterilization device, so as to evaluate the sterilization ability of the sterilization device according to the test sterilization rate. A method for evaluating the sterilization ability of a confined space such as a refrigerator is proposed. And because the initial sterilization rate of the confined space is corrected by the test environment coefficient, the evaluation of the sterilization ability of a confined space such as a refrigerator can be achieved more accurately.

[0064] The first bacterial content of the sterilization liquid set in the sterilization equipment in the sterilization test and the second bacterial content of the control liquid stored in the same environment as the sterilization liquid in the sterilization test are obtained. The results can be obtained in a certain time period during the test or after the test. The certain time period can be the time period with the best sterilization effect after the start of the test, for example, three hours after the start of the test, or five hours after the start of the test.

[0065] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization device, including calculating the test sterilization rate as follows:

[0066] k=f×k 0

[0067] Among them, k is the test sterilization rate, k 0 is the initial sterilization rate, and f is the test environment coefficient.

[0068] In some embodiments, the initial sterilization rate is calculated as follows:

[0069] k 0 =(C1-C2)÷C1

[0070] Among them, k 0is the initial sterilization rate, C1 is the second bacterial content, the unit is colony forming units (cfu) / ml (millilitre) or cfu / g. C2 is the first bacterial content, the unit is cfu / ml or cfu / g.

[0071] In some embodiments, a method for testing the sterilization ability of a sterilization device is provided, comprising:

[0072] Prepare bacterial culture for testing.

[0073] The test bacterial solution was prepared into a control bacterial solution group and a bactericidal bacterial solution group.

[0074] The control bacterial solution group and the bactericidal bacterial solution group were prepared as a control test sample group and a bactericidal test sample group, respectively.

[0075] The sterilization test sample group is placed in the sterilization space of the equipment for sterilization testing to obtain a sterilization sample group after the test; during the sterilization test, the equipment is started to run so that the sterilization space reaches the set test conditions, and the equipment runs for a preset test time.

[0076] Place the control test sample group in a control space with the same volume as the sterilization space of the equipment for control testing to obtain a post-test control sample group; during the control test, make sure that the control space has environmental conditions consistent with the set test conditions for the sterilization test and place it for a preset test time.

[0077] Bacteria were cultured on the post-test sterilization sample group and the post-test control sample group, respectively, to obtain a sterilization test culture group and a control test culture group.

[0078] The first colony information of the sterilization test culture group and the second colony information of the control test culture group are obtained.

[0079] The sterilization capacity of the sterilization space is obtained according to the first colony information and the second colony information.

[0080] In these embodiments, a test and evaluation method for the sterilization effect of the space of household appliances such as refrigerators is proposed. By placing a sterilization test sample group into a sterilization space of the device and placing a control test sample group into a control space with the same volume as the sterilization space of the device, first colony information and second colony information are obtained. Based on the first colony information and the second colony information, the sterilization capacity of the sterilization space is obtained, thereby realizing the test and evaluation of the sterilization effect in the sterilization space of the device.

[0081] In some embodiments, the test bacterial solution is prepared from pasteurized milk.

[0082] In some embodiments, the preparation of the test bacterial solution comprises:

[0083] The pasteurized milk is subjected to rejuvenation culture to obtain a test bacterial solution with a set colony count, wherein the rejuvenation culture includes shaking culture for a first time period, or static culture for a second time period.

[0084] In practical applications, the first duration can be 2 hours to 5 hours, and the second duration can be 12 hours to 24 hours. The set colony count can be 1×10 5 cfu / ml to 1×10 8 cfu / ml.

[0085] In these embodiments, pasteurized milk is used to prepare the test bacterial solution, and colonies are easy to obtain, thereby reducing the disadvantages of difficulty in obtaining professional strains for microbiological operations, high costs, and strict requirements for storage equipment.

[0086] In practical applications, bacterial solutions with various concentrations can be used in experiments to improve the accuracy of the experiments. A bacterial solution with various concentrations can be prepared using a gradient dilution method. In some embodiments, two to three concentrations of bacterial solution can be prepared.

[0087] In some embodiments, preparing a test bacterial solution comprises:

[0088] The pasteurized milk is placed in a triangular flask of a first volume for colony rejuvenation at a first rotation speed.

[0089] The pasteurized milk in the Erlenmeyer flask is shake-cultured at a first temperature for a first period of time.

[0090] The cultured pasteurized milk was diluted according to the gradient dilution method to generate 2 to 3 test bacterial solutions of different concentrations.

[0091] In practical applications, the first volume may be 50 ml, the first temperature may be 30° C., and the first rotation speed may be 100 r / min.

[0092] In some embodiments, in combination Figure 3 As shown, preparing the test bacterial solution includes:

[0093] The pasteurized milk is placed in a triangular flask of a first volume for colony rejuvenation at a first rotation speed.

[0094] The pasteurized milk in the Erlenmeyer flask is incubated at the first temperature for a second period of time.

[0095] The cultured pasteurized milk was diluted according to the gradient dilution method to generate 2 to 3 test bacterial solutions of different concentrations.

[0096] In some embodiments, the control bacterial solution group and the sterilization bacterial solution group are respectively prepared as a control test sample group and a sterilization test sample group, including:

[0097] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread onto a sterilized glass slide or a sterilized filter paper sheet to obtain a first control test sample group and a first sterilization test sample group accordingly.

[0098] Accordingly, a first post-test sterilization sample group and a first post-test control sample group are obtained.

[0099] In this embodiment, it can be understood that the sterilization sample group after the first test is obtained by placing the first sterilization test sample group into the sterilization space of the device, and the device runs the preset test time under the set test conditions. The control sample group after the first test is obtained by placing the first control test sample group into a control space with the same volume as the sterilization space of the device, and making the control space have environmental conditions consistent with the set test conditions, and placing it for the preset test time.

[0100] In some embodiments, the post-test sterilization sample group and the post-test control sample group are subjected to bacterial culture respectively, comprising:

[0101] The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and equilibrated at a second temperature for a third time to obtain a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0102] A first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample are respectively pipetted and added into sterile culture plates.

[0103] A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

[0104] In these embodiments, a method of separately culturing bacteria in the post-test sterilization sample group and the post-test control sample group is proposed.

[0105] In practical applications, the second volume can be 100 ml. The first concentration can be 0.75%. The second temperature can be 20° C. to 30° C. The third duration can be 15 min to 30 min. The first volume can be 15 ml to 25 ml. The third temperature can be 30° C. The fourth duration can be 48 h. During the process of balancing the third duration at the second temperature, the balancing can be performed at a stirring speed of 100 r / min to 150 r / min.

[0106] In some embodiments, the experimental process after preparing the test bacterial solution includes:

[0107] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group were respectively spread onto sterilized glass slides to obtain a first control test sample group and a first sterilization test sample group accordingly.

[0108] The first sterilization test sample group is placed in the sterilization space of the equipment. Under the set test conditions, the equipment runs the preset test time to obtain the first post-test sterilization sample group.

[0109] The first control test sample group is placed in a control space with the same volume as the sterilization space of the equipment, and the environmental conditions in the control space are made consistent with the set test conditions. The preset test time is placed to obtain the first post-test control sample group.

[0110] The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and equilibrated at a second temperature for a third time to obtain a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0111] A first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample are respectively pipetted and added into sterile culture plates.

[0112] A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

[0113] In some embodiments, the experimental process after preparing the test bacterial solution includes:

[0114] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group were spread onto sterilized filter paper sheets respectively, to obtain a first control test sample group and a first sterilization test sample group accordingly.

[0115] The first sterilization test sample group is placed in the sterilization space of the equipment. Under the set test conditions, the equipment runs the preset test time to obtain the first post-test sterilization sample group.

[0116] The first control test sample group is placed in a control space with the same volume as the sterilization space of the equipment, and the environmental conditions in the control space are made consistent with the set test conditions. The preset test time is placed to obtain the first post-test control sample group.

[0117] The first post-test sterilization sample group and the first post-test control sample group are respectively placed in a second volume of physiological saline of a first concentration, and equilibrated at a second temperature for a third time to obtain a first post-test sterilization equilibrium sample group and a first post-test control equilibrium sample group.

[0118] A first volume of the first post-test sterilization equilibrium sample and the first post-test control equilibrium sample are respectively pipetted and added into sterile culture plates.

[0119] A first volume of plate count agar medium is injected into each sterile culture plate, and then the sterile culture plate is placed in an environment of a third temperature and cultured for a fourth time period.

[0120] In some embodiments, the control bacterial solution group and the sterilization bacterial solution group are respectively prepared as a control test sample group and a sterilization test sample group, including:

[0121] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group are respectively spread on the bottom surface of the sterile culture dish or on the agar solidified layer laid on the bottom surface of the sterile culture dish, and a second control test sample group and a second sterilization test sample group are obtained accordingly.

[0122] Accordingly, a second post-test sterilization sample group and a second post-test control sample group are obtained.

[0123] In this embodiment, it can be understood that the second post-test sterilization sample group is obtained by placing the second sterilization test sample group into the sterilization space of the device, and the device runs the preset test time under the set test conditions. The second post-test control sample group is obtained by placing the second control test sample group into a control space with the same volume as the sterilization space of the device, and making the control space have environmental conditions consistent with the set test conditions, and placing it for the preset test time.

[0124] In some embodiments, the post-test sterilization sample group and the post-test control sample group are subjected to bacterial culture respectively, comprising:

[0125] The bacterial nutrient gel suspension or the plate count agar medium is respectively covered on the culture dish surfaces of the second post-test sterilization sample group and the second post-test control sample group; after the bacterial nutrient gel suspension or the plate count agar medium solidifies, it is placed in an environment of a fourth temperature and cultured for a sixth time period.

[0126] The bacterial nutrient gel suspension includes, by weight percentage, 0.2% to 0.6% soy protein, 0.1% to 0.8% trypsin, 0.05% to 0.45% glucose, 2% to 8% sodium chloride, 0.05% to 0.5% dipotassium hydrogen phosphate, 0.2% to 0.9% agar, and water is supplemented to 100%.

[0127] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.2% soy protein, 0.1% trypsin, 0.05% glucose, 2% sodium chloride, 0.05% dipotassium hydrogen phosphate, 0.2% agar, and water is supplemented to 100%.

[0128] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.6% soy protein, 0.8% trypsin, 0.45% glucose, 8% sodium chloride, 0.5% dipotassium hydrogen phosphate, 0.9% agar, and water is supplemented to 100%.

[0129] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.3% soy protein, 0.7% trypsin, 0.3% glucose, 6% sodium chloride, 0.4% dipotassium hydrogen phosphate, and 0.7% agar, and water is supplemented to 100%.

[0130] In some embodiments, the bacterial nutrient gel suspension includes, by weight percentage, 0.4% soy protein, 0.7% trypsin, 0.4% glucose, 7% sodium chloride, 0.5% dipotassium hydrogen phosphate, and 0.8% agar, and water is supplemented to 100%.

[0131] In practical applications, the fourth temperature may be 30° C., and the sixth time period may be 48 hours.

[0132] In some embodiments, the experimental process after preparing the test bacterial solution includes:

[0133] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group were spread on the bottom surface of the sterile culture dish to obtain a second control test sample group and a second sterilization test sample group accordingly.

[0134] The second sterilization test sample group is placed in the sterilization space of the equipment. Under the set test conditions, the equipment runs the preset test time to obtain the second post-test sterilization sample group.

[0135] The second control test sample group is placed in a control space with the same volume as the sterilization space of the equipment, and the environmental conditions in the control space are consistent with the set test conditions. The preset test time is placed to obtain the second post-test control sample group.

[0136] A gel suspension medium containing soybean protein, trypsin, glucose, sodium chloride, potassium hydrogen phosphate, agar and water was prepared.

[0137] The bacterial nutrient gel suspension is respectively covered on the culture dish surfaces of the sterilized sample group after the second test and the control sample group after the second test, and after the bacterial nutrient gel suspension is solidified, it is placed in an environment of a fourth temperature and cultured for a sixth time period.

[0138] In these embodiments, a gel suspension medium containing soybean protein, trypsin, glucose, sodium chloride, potassium hydrogen phosphate, agar and water is prepared and coated on the surface of the plate, thereby reducing the loss effect of the microorganism elution process.

[0139] In some embodiments, the experimental process after preparing the test bacterial solution includes:

[0140] The bacterial solutions of the control bacterial solution group and the sterilization bacterial solution group were spread onto the agar solidified layer laid on the bottom of the sterile culture dish, and the second control test sample group and the second sterilization test sample group were obtained accordingly.

[0141] The second sterilization test sample group is placed in the sterilization space of the equipment. Under the set test conditions, the equipment runs the preset test time to obtain the second post-test sterilization sample group.

[0142] The second control test sample group is placed in a control space with the same volume as the sterilization space of the equipment, and the environmental conditions in the control space are consistent with the set test conditions. The preset test time is placed to obtain the second post-test control sample group.

[0143] The plate count agar medium is respectively covered on the culture dish surfaces of the sterilized sample group after the second test and the control sample group after the second test, and after the bacterial nutrient gel suspension or the plate count agar medium is solidified, it is placed in an environment of a fourth temperature and cultured for a sixth time period.

[0144] In these embodiments, agar and plate count agar medium are used as double-layer medium covering and bottom colony coating. The colony distribution is more uniform, the influence of colony elution is reduced, and since the colony operation space becomes larger, it is more feasible to adjust different dilution gradients.

[0145] In practical applications, the agar solidified layer laid on the bottom of the sterile culture dish is obtained by the following steps:

[0146] Weigh 7g to 25g of agar, add 1000mL of grade 3 pure water to prepare a 0.7% to 2.5% agar suspension, and dispense into Erlenmeyer flasks. Sterilize at 121℃ for 15min to 20min, select a disposable sterile culture plate, pour 15mL to 20mL of sterilized agar solution, and solidify for 1h to 12h.

[0147] In some embodiments, the second colony information includes a second bacterial content. The first colony information includes a first bacterial content. The bacterial content can be obtained by a living cell counting method, an electronic counter counting method, or the like.

[0148] In some embodiments, the test environment coefficient is calculated as follows:

[0149] f=f1, or, f=f2, or, f=f1×f2

[0150] Among them, f is the test environment coefficient, f1 is the time coefficient, and f2 is the space coefficient.

[0151] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization device, including calculating the test sterilization rate as follows:

[0152] k=f1×k 0

[0153] Among them, k is the test sterilization rate, f1 is the time coefficient, k 0 is the initial sterilization rate.

[0154] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization device, including calculating the test sterilization rate as follows:

[0155] k=f2×k 0

[0156] Among them, k is the test sterilization rate, f2 is the space coefficient, k 0 is the initial sterilization rate.

[0157] In some embodiments, the initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization device, including calculating the test sterilization rate as follows:

[0158] k=f1×f2×k 0

[0159] Among them, k is the test sterilization rate, f1 is the time coefficient, f2 is the space coefficient, k 0 is the initial sterilization rate.

[0160] In these embodiments, by calculating the test sterilization rate according to the time coefficient and the space coefficient, the initial sterilization rate can be adjusted according to the test time and the test space, thereby more accurately evaluating the sterilization capacity of a confined space such as a refrigerator.

[0161] The time coefficient is a coefficient calculated based on the test start time and test end time. It is used to correct the initial sterilization rate according to the time required for the test. The value range of the time coefficient is between 0 and 1.

[0162] The space coefficient is a coefficient calculated based on the volume of the confined space where the test is located. It is used to correct the initial sterilization rate based on the volume of the confined space where the test is located. The value range of the space coefficient is between 0 and 1.

[0163] In some implementations, the time factor is calculated as follows:

[0164] f1=a / (T2-T1)

[0165] Among them, f1 is the time coefficient, a is the first adjustment parameter, T2 is the time when the bacterial content is obtained in the sterilization test, and T1 is the preset time in the sterilization test.

[0166] In some embodiments, T1 is the time when the sterilizing solution is placed into the sterilization device.

[0167] In practical applications, the value of a can be 0.5 to 1. In some embodiments, the value of a is 1.

[0168] In some embodiments, the spatial coefficient is calculated as follows:

[0169] f2=b / V

[0170] Among them, f2 is the space coefficient, b is the second adjustment parameter, and V is the test volume of the sterilization equipment.

[0171] In practical applications, the value of b can be 625.

[0172] In practical applications, when evaluating the sterilization capability of a refrigerator's refrigerating chamber, V may be the volume of the refrigerating chamber. When evaluating the sterilization capability of a refrigerator's freezing chamber, V may be the volume of the freezing chamber. When the refrigerating chamber includes a sealed drawer, when evaluating the sterilization capability of the refrigerator's refrigerating chamber, V may be the difference between the volume of the refrigerating chamber and the volume of the drawer.

[0173] In some embodiments, evaluating the sterilization ability of a sterilization device according to a test sterilization rate includes:

[0174] When the tested sterilization rate is greater than or equal to the set sterilization rate, the processor determines that the sterilization equipment has the sterilization capability.

[0175] When the tested sterilization rate is less than the set sterilization rate, the processor determines that the sterilization equipment does not have the sterilization capability.

[0176] In some embodiments, the sterilization rate is set at 90%.

[0177] In some embodiments, the second colony information includes a second bacterial content and a second colony area parameter. The first colony information includes a first bacterial content and a first colony area parameter. Figure 2 As shown, the method for testing the sterilization ability of the sterilization equipment includes the following steps:

[0178] S201. The processor obtains a first bacterial content of a sterilization liquid disposed in a sterilization device in a sterilization test, and a second bacterial content of a control liquid stored in the same environment as the sterilization liquid in a sterilization test.

[0179] S202. The processor determines an initial sterilization rate of the sterilization equipment according to the first bacteria content and the second bacteria content.

[0180] S203. The processor obtains the test environment coefficient of the sterilization equipment.

[0181] S204. The processor uses the test environment coefficient to correct the initial sterilization rate to obtain the test sterilization rate of the sterilization equipment.

[0182] S205. The processor obtains a first colony area parameter of a sterilization test of a sterilization solution set in a sterilization device, and a second colony area parameter of a control bacterial solution having the same storage environment as the sterilization solution in a sterilization test.

[0183] S206. The processor determines a colony area parameter ratio between the first colony area parameter and the second colony area parameter.

[0184] S207. The processor obtains evaluation parameters of the sterilization test.

[0185] S208. The processor determines the sterilization parameters of the sterilization equipment according to the ratio of the evaluation parameters to the colony area parameters, so as to evaluate the sterilization ability of the sterilization equipment according to the test sterilization rate and the sterilization parameters.

[0186] In these embodiments, by evaluating the sterilization ability of the sterilization equipment according to the sterilization parameters and the test sterilization rate, the sterilization ability of a closed space such as a refrigerator can be evaluated more accurately.

[0187] In some embodiments, the sterilization ability of the sterilization equipment is evaluated based on the test sterilization rate and sterilization parameters. When the sterilization equipment is determined to have sterilization ability based on the test sterilization rate and it is determined that the sterilization equipment has obvious sterilization ability based on the sterilization parameters, the sterilization ability of the sterilization equipment is determined to be excellent.

[0188] When it is determined that the sterilization equipment has sterilization ability based on the test sterilization rate, and it is determined that the sterilization equipment has no obvious sterilization ability based on the sterilization parameters, the sterilization ability of the sterilization equipment is determined to be good.

[0189] When the sterilization equipment is determined to have no sterilization ability based on the test sterilization rate, and is determined to have obvious sterilization ability based on the sterilization parameters, the sterilization ability of the sterilization equipment is determined to be medium.

[0190] When it is determined that the sterilization equipment has no sterilization ability based on the test sterilization rate, and when it is determined that the sterilization equipment has no obvious sterilization ability based on the sterilization parameters, the sterilization ability of the sterilization equipment is determined to be poor.

[0191] Among them, the first colony area parameter of the sterilization liquid set in the sterilization equipment in the sterilization test, and the second colony area parameter of the control liquid in the sterilization test in the same storage environment as the sterilization liquid are obtained. It can be obtained in a certain time period during the test, or it can be obtained after the test. Among them, the certain time period can be the time period with the best sterilization effect after the start of the test, for example, three hours after the start of the test, or five hours after the start of the test.

[0192] In some embodiments, in combination Figure 3 As shown, the bactericidal solution and the control solution were prepared as follows:

[0193] S301, the processor performs rejuvenation culture on the pasteurized milk to obtain a bacterial solution with a set colony count, wherein the rejuvenation culture includes shaking culture for a first time period, or static culture for a second time period.

[0194] S302: The processor uses the bacterial solution with a set colony count as the sterilizing bacterial solution.

[0195] S303: The processor uses the bacterial solution with a set colony count as the control bacterial solution.

[0196] In practical applications, the first duration can be 2 hours to 5 hours, and the second duration can be 12 hours to 24 hours. The set colony count can be 1×10 5 cfu / ml to 1×10 8 cfu / ml.

[0197] In these embodiments, pasteurized milk is used to prepare the sterilized bacterial solution and the control bacterial solution. Since pasteurized milk is relatively easy to obtain and relatively inexpensive, the preservation requirements are not high. The colonies of the prepared sterilized bacterial solution are easy to obtain, which reduces the disadvantages of the difficulty in obtaining professional strains for microbial operations, high costs, and strict requirements for storage equipment.

[0198] In practical applications, the sterilization test experiment in the embodiment of the present disclosure includes:

[0199] A control bacterial solution group and a bactericidal bacterial solution group were prepared.

[0200] The sterilization liquid group is placed in the sterilization space of the equipment for sterilization testing to obtain the sterilization liquid group after the test. During the sterilization test, the equipment is started to operate so that the sterilization space reaches the set test conditions, and the equipment runs for a preset test time.

[0201] The control bacterial solution group is placed in a control space with the same volume as the sterilization space of the device for a control test to obtain a control bacterial solution group after the test. During the control test, the control space is made to have environmental conditions consistent with the set test conditions of the sterilization test and is placed for a preset test time.

[0202] Bacterial culture was performed on the post-test bactericidal solution group and the post-test control solution group respectively.

[0203] Combination Figure 4 As shown, the embodiment of the present disclosure also provides a method for testing the sterilization ability of a sterilization device, comprising:

[0204] S401. The processor obtains a first bacterial content of a sterilization test of a sterilization liquid set in a sterilization device, and a second bacterial content of a control liquid stored in the same environment as the sterilization liquid in a sterilization test.

[0205] S402: The processor determines an initial sterilization rate of the sterilization device according to the first bacteria content and the second bacteria content.

[0206] S403: The processor obtains a time coefficient.

[0207] S404: The processor obtains a spatial coefficient.

[0208] S405. The processor uses the time coefficient and the space coefficient to correct the initial sterilization rate to obtain the test sterilization rate of the sterilization equipment.

[0209] S406. The processor obtains a first colony area parameter of a sterilization test of a sterilization solution set in a sterilization device, and a second colony area parameter of a control bacterial solution having the same storage environment as the sterilization solution in a sterilization test.

[0210] S407: The processor determines a colony area parameter ratio between the first colony area parameter and the second colony area parameter.

[0211] S408. The processor obtains evaluation parameters of the sterilization test.

[0212] S409. The processor determines the sterilization parameters of the sterilization equipment according to the ratio of the evaluation parameters to the colony area parameters, so as to evaluate the sterilization ability of the sterilization equipment according to the test sterilization rate and the sterilization parameters.

[0213] In some embodiments, determining the sterilization parameter of the sterilization device according to the ratio of the evaluation parameter to the colony area parameter includes calculating the sterilization parameter according to the following formula:

[0214]

[0215] Among them, f3 is the evaluation parameter, S1 is the first colony area parameter, and S2 is the second colony area parameter.

[0216] Among them, the evaluation parameter is to ensure the effective constant of the sterilization test. In conventional microbiological tests, the difference in colony count is more than 10 times, and the microbial sterilization test is considered effective. Therefore, S2÷S1 must be greater than 10. Set the value of f3 to When k is greater than or equal to 1, it indicates that effective sterilization has been performed. When k is less than 1, it indicates that effective sterilization has not been performed. In some embodiments, the evaluation parameter may be 0.316.

[0217] The first colony area parameter may be the value of the first colony area, or the product of the value of the first colony area and the dilution factor.

[0218] The second colony area parameter may be the value of the second colony area, or the product of the value of the second colony area and the dilution factor.

[0219] In some embodiments, Figure 5 As shown in the figure, the sterilization ability of the sterilization equipment is evaluated according to the sterilization parameters, including:

[0220] S501. When the sterilization parameter is greater than or equal to the set value, the processor determines that the sterilization capacity of the sterilization equipment is a first set capacity.

[0221] S502: When the sterilization parameter is less than a set value, the processor determines that the sterilization capacity of the sterilization equipment is a second set capacity.

[0222] Among them, the sterilization capacity of the first setting capacity is greater than the second setting capacity.

[0223] The set value may be 1. The first set capability may be that there is an obvious sterilization capability. The second set capability may be that there is no obvious sterilization capability.

[0224] In these embodiments, by comparing the sterilization parameters and the set values, the sterilization capability of the sterilization equipment can be determined more accurately.

[0225] In some embodiments, the first colony area parameter and the second colony area parameter are determined as follows:

[0226] S1=N1×A1,S2=N2×A2

[0227] Among them, S1 is the first colony area parameter, N1 is the first dilution factor, A1 is the first colony area score, S2 is the second colony area parameter, N2 is the second dilution factor, and A2 is the second colony area score.

[0228] The dilution factor is the dilution factor of the gradient dilution of the bacterial solution used to calculate the colony area parameter. The colony area parameter is determined by the dilution factor and the colony area score, which can take into account the impact of different dilution factors on the colony area parameter, thereby obtaining a more accurate colony area parameter.

[0229] In some embodiments, colonies of the bactericidal solution and / or the control solution are distributed in multiple cells.

[0230] The processor determines the first colony area score as follows:

[0231]

[0232] Among them, A1 is the first colony area score, Z1 is the first colony area weight of each cell under the area calculation scale, a1 is the first score weight of each cell under the area calculation scale, and n1 is the first total number of cells of the sample under the area calculation scale.

[0233] In practical applications, taking n1 as 5 as an example, the weights of the first colony area of ​​5 cells are Z1 1 =1, Z1 2 =4, Z1 3 =3, Z1 4 =2, Z1 5 =1. The first scoring weights of the five cells are a1 1 =1,a1 2 =0.5, a1 3 =0.25, a1 4 =1,a1 5 =0.5. Then A1 = Z1 1 ×a1 1 +Z1 2 ×a1 2 +Z1 3 ×a1 3 +Z1 4 ×a1 4 +Z1 5 ×a1 5 =1×1+4×0.5+3×0.25+2×1+1×0.5=6.25.

[0234] In some embodiments, the processor determines the second colony area score as follows:

[0235]

[0236] Among them, A2 is the second colony area score, Z2 is the second colony area weight of each cell under the area calculation scale, a2 is the second score weight of each cell under the area calculation scale, and n2 is the second total number of cells of the sample under the area calculation scale.

[0237] In practical applications, taking n2 as 5 as an example, the second colony area weights of the five cells are Z2 1 =1, Z2 2 =4, Z2 3 =3, Z2 4 =2, Z2 5 =1. The first scoring weights of the five cells are a2 1 =1,a2 2 =0.5, a2 3 =0.25, a2 4 =1,a2 5 =0.5. Then A2 = Z2 1 ×a2 1 +Z2 2 ×a2 2 +Z2 3 ×a2 3 +Z24 ×a2 4 +Z2 5 ×a2 5 =1×1+4×0.5+3×0.25+2×1+1×0.5=6.25.

[0238] In some embodiments, the processor determines the first colony area score as follows:

[0239]

[0240] Among them, A1 is the first colony area score, Z1 is the first colony area weight of each cell under the area calculation scale, a1 is the first score weight of each cell under the area calculation scale, and n1 is the first total number of cells of the sample under the area calculation scale.

[0241] The processor determines the second colony area score as follows:

[0242]

[0243] Among them, A2 is the second colony area score, Z2 is the second colony area weight of each cell under the area calculation scale, a2 is the second score weight of each cell under the area calculation scale, and n2 is the second total number of cells of the sample under the area calculation scale.

[0244] In these embodiments, the first colony area score is determined by the first colony area weight of each cell under the area calculation scale and the first scoring weight of each cell under the area calculation scale. The second colony area score is determined according to the second colony area weight of each cell under the area calculation scale and the second scoring weight of each cell under the area calculation scale, and the colony area score is determined according to the colony area weight associated with the colony area of ​​each cell and the first scoring weight associated with the cell area. The influence of different cell areas on the colony area score is taken into account. The first colony area score and the second colony area score can be determined more accurately.

[0245] In some embodiments, the first colony area weight is determined as follows:

[0246] The processor determines a first area ratio of the colonies of the sterilizing liquid in each cell.

[0247] The processor determines a first colony area weight of a cell where a colony of the sterilizing liquid is located according to the first area ratio.

[0248] In some embodiments, the value of the first colony area weight can be determined according to the value of the first area ratio. The value of the first area ratio is proportional to the value of the first colony area weight. In some embodiments, the second colony area weight is determined as follows:

[0249] The processor determines a second area ratio of the colonies of the control bacterial solution in each cell.

[0250] The processor determines a second colony area weight of the cell where the colony of the control bacterial solution is located according to the second area ratio.

[0251] In some implementations, the value of the second colony area weight may be determined according to the value of the second area ratio, and the value of the second area ratio is proportional to the value of the second colony area weight.

[0252] In some embodiments, the first colony area weight is determined as follows:

[0253] The processor determines a first area ratio of the colonies of the sterilizing liquid in each cell.

[0254] The processor determines a first colony area weight of a cell where a colony of the sterilizing liquid is located according to the first area ratio.

[0255] The second colony area weight is determined as follows:

[0256] The processor determines a second area ratio of the colonies of the control bacterial solution in each cell.

[0257] The processor determines the second colony area weight of the cell where the colony of the sterilizing liquid is located according to the second area ratio. In these embodiments, the first colony area weight can be determined by the first area ratio. The second colony area weight can be determined by the second area ratio. Thus, the first colony area weight and the second colony area weight can reflect the colony area ratio of each cell.

[0258] In some embodiments, the first colony area weight is determined as follows:

[0259] When the first area ratio is greater than or equal to three quarters, the first colony area weight is the first area weight value.

[0260] When the first area accounts for less than three quarters and is greater than or equal to one half, the first colony area weight is the second area weight value.

[0261] When the first area accounts for less than one half and is greater than or equal to one quarter, the first colony area weight is the third area weight value.

[0262] When the first area accounts for less than one quarter, the first colony area weight is the fourth area weight value.

[0263] In some embodiments, the second colony area weight is determined as follows:

[0264] When the second area ratio is greater than or equal to three quarters, the second colony area weight is the first area weight value.

[0265] When the second area proportion is less than three quarters and greater than or equal to one half, the second colony area weight is the second area weight value.

[0266] When the second area ratio is less than one half and greater than or equal to one quarter, the second colony area weight is the third area weight value.

[0267] When the second area accounts for less than one quarter, the second colony area weight is the fourth area weight value.

[0268] In actual applications, the first area weight value is 4, the second area weight value is 3, the third area weight value is 2, and the fourth area weight value is 1.

[0269] In some implementations, the first scoring weight is determined as follows:

[0270] The processor determines a first ratio of the area of ​​each cell to the area of ​​a standard cell.

[0271] The processor determines a first scoring weight of a cell where a colony of the bactericidal liquid is located according to the first ratio.

[0272] In some implementations, the second scoring weight is determined as follows:

[0273] The processor determines a second ratio of the area of ​​each cell to the area of ​​the standard cell.

[0274] The processor determines a second scoring weight of the cell where the colony of the control bacterial solution is located according to the second ratio.

[0275] In some implementations, the first scoring weight is determined as follows:

[0276] The processor determines a first ratio of the area of ​​each cell to the area of ​​a standard cell.

[0277] The processor determines a first scoring weight of a cell where a colony of the bactericidal liquid is located according to the first ratio.

[0278] The second scoring weight is determined as follows:

[0279] The processor determines a second ratio of the area of ​​each cell to the area of ​​the standard cell.

[0280] The processor determines a second scoring weight of the cell where the colony of the control bacterial solution is located according to the second ratio.

[0281] The value of the first ratio is proportional to the first scoring weight, and the value of the second ratio is proportional to the second scoring weight.

[0282] In these embodiments, the first scoring weight is determined according to a first ratio of each cell area to the standard cell area, and the second scoring weight is determined according to a second ratio of each cell area to the standard cell area. The first scoring weight and the second scoring weight can reflect the ratio of each cell area to the standard cell area.

[0283] In practical applications, the first scoring weight can be determined as follows:

[0284] When the cell area is greater than half of the standard cell area, the first scoring weight is the first weight value.

[0285] When the cell area is less than or equal to half of the standard cell area and greater than one quarter of the standard cell area, the first scoring weight is the second weight value.

[0286] When the cell area is less than or equal to one quarter of the standard cell area, the first scoring weight is the third weight value.

[0287] In practical applications, the second scoring weight can be determined as follows:

[0288] When the cell area is greater than half of the standard cell area, the second scoring weight is the first weight value.

[0289] When the cell area is less than or equal to one-half of the standard cell area and greater than one-quarter of the standard cell area, the second scoring weight is the second weight value.

[0290] When the cell area is less than or equal to one quarter of the standard cell area, the second scoring weight is the third weight value.

[0291] In actual applications, the first weight value is 1, the second weight value is 0.5, and the third weight value is 0.25.

[0292] Combination Figure 6As shown, an embodiment of the present disclosure provides a device for testing the sterilization ability of a sterilization device, including a processor (processor) 600 and a memory (memory) 601. Optionally, the device may also include a communication interface (Communication Interface) 602 and a bus 603. Among them, the processor 600, the communication interface 602, and the memory 601 can communicate with each other through the bus 603. The communication interface 602 can be used for information transmission. The processor 600 can call the logic instructions in the memory 601 to execute the method for testing the sterilization ability of a sterilization device in the above embodiment.

[0293] In addition, the logic instructions in the memory 601 described above may be implemented in the form of software functional units and when sold or used as independent products, may be stored in a computer-readable storage medium.

[0294] The memory 601 is a computer-readable storage medium that can be used to store software programs and computer executable programs, such as program instructions / modules corresponding to the method in the embodiment of the present disclosure. The processor 600 executes the functional application and data processing by running the program instructions / modules stored in the memory 601, that is, the method for testing the sterilization ability of the sterilization equipment in the above embodiment is implemented.

[0295] The memory 601 may include a program storage area and a data storage area, wherein the program storage area may store an operating system and an application required for at least one function. The data storage area may store data created according to the use of the terminal device, etc. In addition, the memory 601 may include a high-speed random access memory and may also include a non-volatile memory.

[0296] The embodiments of the present disclosure provide a testing device, including: a device body, and the above-mentioned device for testing the sterilization ability of the sterilization device. The device for testing the sterilization ability of the sterilization device is installed on the device body. The installation relationship described here is not limited to placement inside the product, but also includes installation connections with other components of the product, including but not limited to physical connections, electrical connections, or signal transmission connections. It can be understood by those skilled in the art that the device for testing the sterilization ability of the sterilization device can be adapted to a feasible refrigeration device body, thereby realizing other feasible embodiments.

[0297] An embodiment of the present disclosure provides a computer program, which, when executed by a computer, enables the computer to implement the above-mentioned method for testing the sterilization ability of a sterilization device.

[0298] An embodiment of the present disclosure provides a computer program product, which includes computer instructions stored on a computer-readable storage medium. When the program instructions are executed by a computer, the computer implements the above-mentioned method for testing the sterilization ability of a sterilization device.

[0299] An embodiment of the present disclosure provides a computer-readable storage medium storing computer-executable instructions, wherein the computer-executable instructions are configured to execute the above-mentioned method for testing the sterilization capability of a sterilization device.

[0300] The computer-readable storage medium mentioned above may be a transient computer-readable storage medium or a non-transitory computer-readable storage medium.

[0301] The method, device and testing equipment for testing the sterilization ability of sterilization equipment provided by the embodiments of the present disclosure can achieve the following technical effects:

[0302] The initial sterilization rate of the sterilization equipment is determined according to the first bacterial content of the sterilization liquid in the sterilization test and the second bacterial content of the control liquid in the same storage environment as the sterilization liquid in the sterilization test, and the initial sterilization rate is corrected by the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, so as to evaluate the sterilization ability of the sterilization equipment according to the test sterilization rate. The sterilization ability of a confined space such as a refrigerator can be evaluated more accurately.

[0303] The technical solution of the embodiment of the present disclosure can be embodied in the form of a software product, which is stored in a storage medium and includes one or more instructions for enabling a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method described in the embodiment of the present disclosure. The aforementioned storage medium may be a non-transient storage medium, including: a USB flash drive, a mobile hard disk, a read-only memory (ROM), a random access memory (RAM), a magnetic disk or an optical disk, and other media that can store program codes, or a transient storage medium.

[0304] The above description and the accompanying drawings sufficiently illustrate the embodiments of the present disclosure to enable those skilled in the art to practice them. Other embodiments may include structural, logical, electrical, process, and other changes. The embodiments represent only possible variations. Unless explicitly required, individual components and functions are optional, and the order of operations may vary. Portions and features of some embodiments may be included in or replace portions and features of other embodiments. Moreover, the terms used in this application are only used to describe the embodiments and are not used to limit the claims. As used in the description of the embodiments and the claims, the singular forms "a" and "an" shall be construed as "a", "an" and "an" unless the context clearly indicates otherwise.

[0305] (a), "an" and "the" are intended to include plural forms as well. Similarly, the term "and / or" as used in this application is intended to include any and all possible combinations of one or more of the associated listed items. In addition, when used in this application, the term "including"

[0306] "comprise" and its variations "comprises" and / or comprising refer to the presence of stated features, wholes, steps, operations, elements, and / or components, but do not exclude the presence or addition of one or more other features, wholes, steps, operations, elements, components and / or groups thereof. In the absence of further restrictions, the elements defined by the sentence "comprise one..." do not exclude the presence of other identical elements in the process, method or device including the elements. In this article, each embodiment may focus on the differences from other embodiments, and the same and similar parts between the various embodiments may refer to each other. For the methods, products, etc. disclosed in the embodiments, if they correspond to the method part disclosed in the embodiments, then the relevant parts can refer to the description of the method part.

[0307] Those skilled in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software may depend on the specific application and design constraints of the technical solution. The technicians may use different methods for each specific application to implement the described functions, but such implementations should not be considered to exceed the scope of the embodiments of the present disclosure. The technicians may clearly understand that, for the convenience and simplicity of description, the specific working processes of the systems, devices and units described above may refer to the corresponding processes in the aforementioned method embodiments, and will not be repeated here.

[0308] In the embodiments disclosed herein, the disclosed methods and products (including but not limited to devices, equipment, etc.) can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the units can be only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the coupling or direct coupling or communication connection between each other shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms. The units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the units may be selected according to actual needs to implement this embodiment. In addition, each functional unit in the embodiment of the present disclosure may be integrated in a processing unit, or each unit may exist physically alone, or two or more units may be integrated in one unit.

[0309] The flowchart and block diagram in the accompanying drawings show the possible architecture, function and operation of the system, method and computer program product according to the embodiment of the present disclosure. In this regard, each box in the flowchart or block diagram can represent a module, a program segment or a part of the code, and the module, the program segment or a part of the code contains one or more executable instructions for realizing the specified logical function. In some alternative implementations, the functions marked in the box can also occur in a different order from the order marked in the accompanying drawings. For example, two consecutive boxes can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, which can depend on the functions involved. In the description corresponding to the flowchart and the block diagram in the accompanying drawings, the operations or steps corresponding to different boxes can also occur in a different order from the order disclosed in the description, and sometimes there is no specific order between different operations or steps. For example, two consecutive operations or steps can actually be executed substantially in parallel, and they can sometimes be executed in the opposite order, which can depend on the functions involved. Each block in the block diagrams and / or flowcharts, and combinations of blocks in the block diagrams and / or flowcharts, may be implemented by a dedicated hardware-based system that performs the specified functions or actions, or may be implemented by a combination of dedicated hardware and computer instructions.

Claims

1. A method for testing the sterilization ability of a sterilization device, characterized in that: include: Obtaining a first bacterial content of a sterilization test of a sterilization liquid disposed in a sterilization device, and a second bacterial content of a control liquid stored in the same environment as the sterilization liquid in a sterilization test; determining an initial sterilization rate of the sterilization device according to the first bacteria content and the second bacteria content; Obtain the test environment coefficient of the sterilization equipment; The initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, so as to evaluate the sterilization ability of the sterilization equipment based on the test sterilization rate.

2. The method according to claim 1, characterized in that The initial sterilization rate is corrected using the test environment coefficient to obtain the test sterilization rate of the sterilization equipment, including calculating the test sterilization rate in the following manner: k=f×k0 Among them, k is the test sterilization rate, k0 is the initial sterilization rate, and f is the test environment coefficient.

3. The method according to claim 2, characterized in that Calculate the test environment factor as follows: f=f1, or, f=f2, or, f=f1×f2 Among them, f is the test environment coefficient, f1 is the time coefficient, and f2 is the space coefficient.

4. The method according to claim 3, characterized in that The time factor is calculated as follows: f1=a / (T2-T1) Among them, f1 is the time coefficient, a is the first adjustment parameter, T2 is the time when the bacterial content is obtained in the sterilization test, and T1 is the preset time in the sterilization test.

5. The method according to claim 3, characterized in that: The space factor is calculated as follows: f2=b / V Among them, f2 is the space coefficient, b is the second adjustment parameter, and V is the test volume of the sterilization equipment.

6. The method according to any one of claims 1 to 5, characterized in that: Evaluate the sterilization capability of sterilization equipment based on the test sterilization rate, including: When the test sterilization rate is greater than or equal to the set sterilization rate, it is determined that the sterilization equipment has the sterilization capability; When the tested sterilization rate is lower than the set sterilization rate, it is determined that the sterilization equipment does not have the sterilization capability.

7. The method according to any one of claims 1 to 5, characterized in that: The sterilization capacity of the sterilization equipment is evaluated based on the test sterilization rate, including: Obtaining a first colony area parameter of a sterilization test of a sterilization liquid set in a sterilization device, and a second colony area parameter of a control bacterial liquid stored in the same environment as the sterilization liquid in a sterilization test; Determining a colony area parameter ratio of the first colony area parameter and the second colony area parameter; Obtain evaluation parameters for bactericidal test experiments; The sterilization parameters of the sterilization equipment are determined based on the ratio of the evaluation parameters to the colony area parameters, so as to evaluate the sterilization ability of the sterilization equipment based on the test sterilization rate and sterilization parameters.

8. The method according to any one of claims 1 to 5, characterized in that: Prepare the bactericidal solution and / or control solution as follows: Performing rejuvenation culture on the pasteurized milk to obtain a bacterial solution with a set colony count; wherein the rejuvenation culture includes shaking culture for a first time period, or static culture for a second time period; The bacterial solution with a set colony count is used as a sterilization bacterial solution; and / or the bacterial solution with a set colony count is used as a control bacterial solution.

9. A device for testing the sterilization ability of a sterilization device, comprising a processor and a memory storing program instructions, characterized in that: The processor is configured to execute the method for testing the sterilization ability of a sterilization device as described in any one of claims 1 to 8 when executing the program instructions.

10. A testing device, characterized in that: include: Equipment body; The device for testing the sterilization ability of a sterilization device as described in claim 9 is arranged on the device body.

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