In-vitro device for measuring biological accessibility of hydrophobic organic pollutants in soil

Abstract

The invention discloses an in-vitro device for measuring biological accessibility of hydrophobic organic pollutants in soil, and relates to the field of in-vitro devices for biological accessibility of organic pollutants, the in-vitro device comprises an in-vitro device body, the inner wall of the in-vitro device body is fixedly connected with a catalyst box and a soil box, the surface of the in-vitro device body is fixedly connected with a constant temperature controller, and the constant temperature controller is fixedly connected with the soil box. A thermometer and an L-shaped supporting plate are fixedly connected to the upper surface of the in-vitro device, and a solubilizing quantity control mechanism is fixedly connected to the upper surface of the L-shaped supporting plate. When the material containing groove rotates to the opening facing downwards, the material containing groove falls off under the action of gravity, the proportion of soil in the soil box to a surfactant in the catalyst box is controlled by rotating the number of turns of the discharging roller in the corresponding position, mixing is conducted after the soil enters the in-vitro device, various proportion parameters of mixing and solubilizing can be adjusted during experimental determination, and the measurement accuracy is improved. Therefore, whether the solubilizing effect can improve the bioavailability of pollutants or not can be judged.

Description

Technical Field

[0001] The present invention relates to the field of in vitro devices for determining the bioaccessibility of organic pollutants, and in particular to an in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil. Background Art

[0002] The removal of hydrophobic organic pollutants in soil and groundwater systems has always been a difficult problem in the field of environmental remediation. The study of the bioavailability of hydrophobic organic pollutants (HOCs) has always been one of the hot issues. Surfactants have superior properties and their role in environmental remediation is becoming more and more obvious. It is currently known that surfactants can increase the bioavailability of pollutants.

[0003] In existing experiments, the anionic surfactant SDBS is often used as a soil treatment surfactant to study the degradability of hexadecane as a pollutant in the soil. In experimental measurements, it is necessary to consider whether the solubilization effect can improve the bioavailability of pollutants. Therefore, an in vitro device for the bioavailability of organic pollutants is needed to control the various parameters of solubilization, and further verify it in subsequent SEM electron microscope images. Summary of the invention

[0004] The object of the present invention is to provide an in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil, so as to solve the problem proposed in the above background technology that in existing experiments, anionic surfactant SDBS is often selected as a soil treatment surfactant to study the degradability of hexadecane as a pollutant in soil. During the experimental determination, it is necessary to consider whether the solubilization effect can improve the bioavailability of the pollutants. Therefore, an in vitro device for the bioaccessibility of organic pollutants is needed to control the various parameters of solubilization and further verify them in subsequent SEM electron microscope images.

[0005] To achieve the above object, the present invention provides the following technical solution: an in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil, comprising an in vitro device, the inner wall of which is fixedly connected to a catalyst box and a soil box, the surface of which is fixedly connected to a constant temperature controller, the upper surface of which is fixedly connected to a temperature gauge and an L-shaped support plate, and the upper surface of which is fixedly connected to a solubilization control mechanism;

[0006] The solubilization and control mechanism includes a reduction motor fixedly connected to the upper surface of the L-shaped support plate, the output end of the reduction motor passes through the lower surface of the L-shaped support plate and is fixedly connected to a first synchronous wheel, the inner wall of the in vitro device is fixedly connected to a lightweight gear, the inner wall of the lightweight gear is sleeved with a connecting plate, the lower surface of the connecting plate is fixedly connected to a first stirring shaft, and the inner wall of the connecting plate is rotatably connected to a plurality of connecting shafts.

[0007] The top end of the connecting plate is fixedly connected with a second synchronous wheel, the second synchronous wheel is installed above the extracorporeal device, and the surfaces of the L-shaped supporting plate and the second synchronous wheel are sleeved with a same synchronous belt.

[0008] The top end of the connecting shaft is fixedly connected with a first spur gear, which meshes with a lightweight gear. The bottom end of the connecting shaft is fixedly connected with a second spur gear. The lower surface of the connecting plate is rotatably connected with a third spur gear, which meshes with the third spur gear.

[0009] A toggle plate is fixedly connected to the lower surface of the third spur gear, and a second stirring shaft is fixedly connected to the lower surface of the toggle plate.

[0010] The upper surface of the soil box is rotatably connected to a first cover plate, the upper surface of the catalyst box is rotatably connected to a second cover plate, the inner walls of the catalyst box and the soil box are provided with sloped surfaces, the inner walls of the catalyst box and the soil box are provided with adaptive curved surfaces, and the inner walls of the catalyst box and the soil box are rotatably connected to unloading rollers.

[0011] A knob is fixedly connected to one side of the unloading roller, the surface of the unloading roller is in contact with the surface of the matching cambered surface, and a material containing groove is provided on the surface of the unloading roller.

[0012] An electric telescopic rod is fixedly connected to the lower surface of the first cover plate, and a C-shaped plate is fixedly connected to the output end of the electric telescopic rod.

[0013] A threaded groove is formed on the inner wall of the extracorporeal device, a stripping plate is threadedly connected to the inner wall of the extracorporeal device, an O-ring is fixedly connected to the surface of the stripping plate, and a twisting operation plate is fixedly connected to the lower surface of the stripping plate.

[0014] In summary, the technical effects and advantages of the present invention are as follows:

[0015] 1. In the present invention, the rotation of the connecting plate can drive the first spur gear to rotate, and the first spur gear follows the rotation of the connecting plate so that the first spur gear fits the surface of the lightweight gear during the movement, thereby driving the first spur gear to rotate together with the connecting shaft, and the second spur gear is meshed with the third spur gear. The rotation of the second spur gear drives the third spur gear to rotate, and the rotation of the third spur gear drives the toggle plate to rotate together. The rotation of the toggle plate drives the second stirring shaft to rotate, and then drives the multiple connected connecting shafts to rotate together with the first stirring shaft fixedly connected to the lower surface of the connecting plate. The rotation of the second stirring shaft and the first stirring shaft jointly drives the soil and surfactant located in the in vitro device to be mixed and stirred, so as to better perform mixing, mixing, solubilization and regulation.

[0016] 2. In the present invention, during stirring, the operator can rotate the knobs corresponding to the soil box and the catalyst box. The rotation of the knob drives the feed roller to rotate. The feed roller rotates the object in the material tank. When the material tank rotates to the point where the opening faces downward, the object falls off under the force of gravity. By rotating the number of turns of the feed roller in the corresponding position, the ratio between the soil in the soil box and the surfactant in the catalyst box is controlled. After entering the in vitro device, the mixture is mixed. During experimental measurement, the various proportion parameters of the mixed solubilization can be adjusted to determine whether the solubilization effect can improve the effect of the bioavailability of pollutants. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of an embodiment of the present invention;

[0019] Figure 2 It is a schematic cross-sectional structural diagram of an extracorporeal device in an embodiment of the present invention;

[0020] Figure 3 For the embodiment of the present invention Figure 2 A is a schematic diagram of the enlarged structure;

[0021] Figure 4 is a schematic cross-sectional structural diagram of a connecting plate in an embodiment of the present invention;

[0022] Figure 5 It is a schematic diagram of the three-dimensional structure of the thread groove in an embodiment of the present invention;

[0023] Figure 6 Schematic diagram of the planar structure of the soil box in the embodiment of the present invention.

[0024] In the figure: 1. in vitro device; 2. constant temperature controller; 3. temperature gauge; 4. L-shaped support plate; 5. reduction motor; 6. second synchronous wheel; 7. catalyst box; 8. soil box; 9. slope surface; 10. first synchronous wheel; 11. synchronous belt; 12. lightweight gear; 13. first spur gear; 14. connecting plate; 15. toggle plate; 16. second stirring shaft; 17. stripping plate; 18. twisting operation plate; 19. first stirring shaft; 20. O-ring; 21. connecting shaft; 22. second spur gear; 23. third spur gear; 24. first cover plate; 25. second cover plate; 26. knob; 27. unloading roller; 28. threaded groove; 29. ​​electric telescopic rod; 30. C-shaped plate; 31. matching arc surface; 32. material trough. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Example: Reference Figure 1-Figure 6 The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil shown in the figure comprises an in vitro device 1, the inner wall of the in vitro device 1 is fixedly connected to a catalyst box 7 and a soil box 8, the surface of the in vitro device 1 is fixedly connected to a constant temperature controller 2, the upper surface of the in vitro device 1 is fixedly connected to a temperature gauge 3 and an L-shaped support plate 4, and the upper surface of the L-shaped support plate 4 is fixedly connected to a solubilization control mechanism;

[0027] The solubilization and control mechanism includes a reduction motor 5 fixedly connected to the upper surface of the L-shaped support plate 4, the output end of the reduction motor 5 passes through the lower surface of the L-shaped support plate 4 and is fixedly connected to the first synchronous wheel 10, the inner wall of the in vitro device 1 is fixedly connected to a lightweight gear 12, the inner wall of the lightweight gear 12 is sleeved with a connecting plate 14, the lower surface of the connecting plate 14 is fixedly connected to a first stirring shaft 19, and the inner wall of the connecting plate 14 is rotatably connected to a plurality of connecting shafts 21.

[0028] By means of the above structure, a catalyst box 7 is provided to store surfactants, a soil box 8 is provided to place soil or sewage to be measured, an in vitro device 1 is provided, and the in vitro device 1 solubilizes and stirs the soil and surfactant so that the surfactant can react and mix with the soil or sewage better. A constant temperature controller 2 is provided to control and maintain the constant temperature inside the in vitro device 1. A thermometer 3 is provided to understand the internal temperature change. An L-shaped support plate 4 is provided to support and install a reduction motor 5. The reduction motor 5 is provided to start the reduction motor 5, and its output end drives the first synchronous wheel 10 to rotate. A lightweight gear 12 is provided to reduce the weight of the lightweight gear 12 itself, and the first spur gear 13 can be driven to rotate. A connecting plate 14 is provided, and the connecting plate 14 rotates with the lightweight gear 12, thereby driving the multiple connected connecting shafts 21 and the lower surface of the connecting plate 14 to be fixedly connected with the first stirring shaft 19 to rotate together. The rotation of the first stirring shaft 19 drives the soil and the surfactant in the in vitro device 1 to mix and stir. The connecting shaft 21 is provided to maintain the connection support of the first spur gear 13 and the second spur gear 22.

[0029] As a preferred implementation in this embodiment, the top of the connecting plate 14 is fixedly connected to a second synchronous wheel 6, the second synchronous wheel 6 is installed above the extracorporeal device 1, and the surface of the L-shaped support plate 4 and the second synchronous wheel 6 is sleeved with the same synchronous belt 11.

[0030] By providing the synchronous belt 11 , the first synchronous wheel 10 drives the second synchronous wheel 6 to rotate through the synchronous belt 11 , and the rotation of the second synchronous wheel 6 drives the connecting plate 14 connected thereto to rotate.

[0031] As a preferred implementation scheme in this embodiment, the top end of the connecting shaft 21 is fixedly connected to the first spur gear 13, the first spur gear 13 is meshed with the lightweight gear 12, the bottom end of the connecting shaft 21 is fixedly connected to the second spur gear 22, the lower surface of the connecting plate 14 is rotatably connected to the third spur gear 23, the second spur gear 22 is meshed with the third spur gear 23.

[0032] By setting the first spur gear 13 to mesh with the lightweight gear 12, the first spur gear 13 rotates following the connecting plate 14 so that the first spur gear 13 fits the surface of the lightweight gear 12 during movement, thereby driving the first spur gear 13 to rotate together with the connecting shaft 21, and by setting the second spur gear 22 to mesh with the third spur gear 23, the rotation of the second spur gear 22 drives the third spur gear 23 to rotate, and the rotation of the third spur gear 23 drives the toggle plate 15 to rotate together.

[0033] In this embodiment, a toggle plate 15 is fixedly connected to the lower surface of the third spur gear 23 , and a second stirring shaft 16 is fixedly connected to the lower surface of the toggle plate 15 .

[0034] By providing the second stirring shaft 16 , the second stirring shaft 16 rotates along with the toggle plate 15 and cooperates with the first stirring shaft 19 , so that the soil placed in the extracorporeal device 1 and the surfactant are mixed more evenly.

[0035] As a preferred implementation manner in this embodiment, the upper surface of the soil box 8 is rotatably connected to the first cover plate 24, the upper surface of the catalyst box 7 is rotatably connected to the second cover plate 25, the inner walls of the catalyst box 7 and the soil box 8 are provided with a slope surface 9, the inner walls of the catalyst box 7 and the soil box 8 are provided with an adapting arc surface 31, and the inner walls of the catalyst box 7 and the soil box 8 are rotatably connected to a feed roller 27.

[0036] By providing the first cover plate 24, the soil placed in the soil box 8 is protected. By providing the second cover plate 25, the surfactant placed in the catalyst box 7 is better protected. By providing the slope surface 9, the substances placed in the catalyst box 7 and the soil box 8 can slide better through the slope surface 9 into the in vitro device 1 to participate in stirring.

[0037] In this embodiment, a knob 26 is fixedly connected to one side of the unloading roller 27 , the surface of the unloading roller 27 is in contact with the surface of the matching arc surface 31 , and a material receiving groove 32 is provided on the surface of the unloading roller 27 .

[0038] By setting the knob 26, the operator turns the knob 26, and the rotation of the knob 26 drives the unloading roller 27 to rotate. By setting the adapting arc surface 31, it fits with the surface of the unloading roller 27 to achieve a sealing effect, thereby controlling the flow of objects in the catalyst box 7 and the soil box 8. By setting the material trough 32, when the opening of the material trough 32 faces downward, the material in the material trough 32 falls off under the action of gravity through the rotating unloading roller 27.

[0039] In this embodiment, an electric telescopic rod 29 is fixedly connected to the lower surface of the first cover plate 24 , and a C-shaped plate 30 is fixedly connected to the output end of the electric telescopic rod 29 .

[0040] By setting up the electric telescopic rod 29 and starting the electric telescopic rod 29, the output end of the electric telescopic rod 29 can drive the C-shaped plate 30 to drop in height. At this time, after the surface of the C-shaped plate 30 contacts the soil in the soil box 8, the soil is pushed into the material trough 32 in the unloading roller 27 at the corresponding position.

[0041] As a preferred implementation manner in this embodiment, a threaded groove 28 is opened on the inner wall of the extracorporeal device 1, and a stripping plate 17 is threadedly connected to the inner wall of the extracorporeal device 1. An O-ring 20 is fixedly connected to the surface of the stripping plate 17, and a twisting operating plate 18 is fixedly connected to the lower surface of the stripping plate 17.

[0042] By setting a threaded groove 28, which cooperates with the thread of the stripping plate 17, the positioning stability is maintained. By setting an O-ring 20, the seal is maintained when stirring soil or sewage. By setting a twisting operating plate 18, the operator can turn the twisting operating plate 18 to open the stripping plate 17 and take out the stirred soil or sludge on the stripping plate 17.

[0043] The working principle of the present invention is: an in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil. Before the determination, the soil is placed in a soil box 8, and the surfactant is placed in a catalyst box 7. During the determination, the reduction motor 5 is started, and the output end of the reduction motor 5 drives the first synchronous wheel 10 to rotate. The first synchronous wheel 10 drives the second synchronous wheel 6 to rotate through a synchronous belt 11. The rotation of the second synchronous wheel 6 drives the connecting plate 14 connected thereto to rotate. The rotation of the connecting plate 14 can drive the first spur gear 13 to rotate. The first spur gear 13 rotates following the rotation of the connecting plate 14 so that the first spur gear 13 fits the surface of the lightweight gear 12 during the movement, thereby driving the first spur gear 13 to rotate together with the connecting shaft 21, the second spur gear 22 is meshed with the third spur gear 23, the rotation of the second spur gear 22 drives the third spur gear 23 to rotate, the rotation of the third spur gear 23 drives the toggle plate 15 to rotate together, the rotation of the toggle plate 15 drives the second stirring shaft 16 to rotate, thereby driving the connected multiple connecting shafts 21 and the connecting shaft 21 to rotate together. The lower surface of the connecting plate 14 is fixedly connected with a first stirring shaft 19 to rotate together. The rotation of the second stirring shaft 16 and the first stirring shaft 19 jointly drives the soil and surfactant located in the in vitro device 1 to be mixed and stirred. During stirring, the operator can rotate the knob 26 corresponding to the soil box 8 and the catalyst box 7. The rotation of the knob 26 drives the feed roller 27 to rotate. The feed roller 27 rotates the object located in the material storage tank 32 to rotate. When the material storage tank 32 rotates to the opening facing downward, it falls off under the force of gravity. By rotating the number of turns of the feed roller 27 in the corresponding position, the ratio between the soil in the soil box 8 and the surfactant in the catalyst box 7 is controlled. After entering the in vitro device 1, mixing is carried out. When sampling, the operator rotates and twists the operating plate 18 to open the stripping plate 17 and take out the soil or sludge that has been stirred on the stripping plate 17. With the above structure, various proportion parameters of mixed solubilization can be adjusted during experimental measurement to determine whether the solubilization effect can improve the effect of bioavailability of pollutants.

[0044] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An in vitro device for determining the bioaccessibility of hydrophobic organic contaminants in soil, comprising an in vitro device (1), characterized in that: The inner wall of the in vitro device (1) is fixedly connected to a catalyst box (7) and a soil box (8), the surface of the in vitro device (1) is fixedly connected to a constant temperature controller (2), the upper surface of the in vitro device (1) is fixedly connected to a temperature gauge (3) and an L-shaped support plate (4), and the upper surface of the L-shaped support plate (4) is fixedly connected to a solubilization control mechanism; The solubilization control mechanism comprises a reduction motor (5) fixedly connected to the upper surface of an L-shaped support plate (4); the output end of the reduction motor (5) passes through the lower surface of the L-shaped support plate (4) and is fixedly connected to a first synchronous wheel (10); the inner wall of the in vitro device (1) is fixedly connected to a lightweight gear (12); the inner wall of the lightweight gear (12) is sleeved with a connecting plate (14); the lower surface of the connecting plate (14) is fixedly connected to a first stirring shaft (19); and the inner wall of the connecting plate (14) is rotatably connected to a plurality of connecting shafts (21).

2. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 1, characterized in that: The top end of the connecting plate (14) is fixedly connected to a second synchronous wheel (6), the second synchronous wheel (6) is installed above the extracorporeal device (1), and the surfaces of the L-shaped supporting plate (4) and the second synchronous wheel (6) are sleeved with a same synchronous belt (11).

3. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 1, characterized in that: The top end of the connecting shaft (21) is fixedly connected to a first spur gear (13), the first spur gear (13) is meshed with a lightweight gear (12), the bottom end of the connecting shaft (21) is fixedly connected to a second spur gear (22), the lower surface of the connecting plate (14) is rotatably connected to a third spur gear (23), the second spur gear (22) is meshed with the third spur gear (23).

4. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 3, characterized in that: The lower surface of the third spur gear (23) is fixedly connected to a toggle plate (15), and the lower surface of the toggle plate (15) is fixedly connected to a second stirring shaft (16).

5. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 1, characterized in that: The upper surface of the soil box (8) is rotatably connected to a first cover plate (24), the upper surface of the catalyst box (7) is rotatably connected to a second cover plate (25), and the inner walls of the catalyst box (7) and the soil box (8) are provided with slope surfaces (9).

6. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 5, characterized in that: The inner walls of the catalyst box (7) and the soil box (8) are provided with an adapting cambered surface (31), and the inner walls of the catalyst box (7) and the soil box (8) are rotatably connected with a feeding roller (27).

7. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 6, characterized in that: A knob (26) is fixedly connected to one side of the unloading roller (27), the surface of the unloading roller (27) is in contact with the surface of the matching cambered surface (31), and a material receiving groove (32) is provided on the surface of the unloading roller (27).

8. The in vitro device for determining the bioaccessibility of hydrophobic organic contaminants in soil according to claim 6, characterized in that: The lower surface of the first cover plate (24) is fixedly connected to an electric telescopic rod (29), and the output end of the electric telescopic rod (29) is fixedly connected to a C-shaped plate (30).

9. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 1, characterized in that: The inner wall of the extracorporeal device (1) is provided with a thread groove (28), and the inner wall of the extracorporeal device (1) is threadedly connected with a stripping plate (17).

10. The in vitro device for determining the bioaccessibility of hydrophobic organic pollutants in soil according to claim 9, characterized in that: An O-shaped sealing ring (20) is fixedly connected to the surface of the stripping plate (17), and a twisting operation plate (18) is fixedly connected to the lower surface of the stripping plate (17).

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