Viable bacteria super-poly-selenium composition for inhibiting growth and division of cancer cells

Abstract

The invention discloses a viable bacterium super-poly-selenium composition for inhibiting growth and division of cancer cells, the composition is obtained by compounding viable bacteria and super-poly-selenium, the activity of pkc in tumor cells can be inhibited through selenium, and meanwhile, the growth and proliferation of the tumor cells can be inhibited; the tumor inhibition mechanism of selenium is to induce the change of cell circulating protein, prevent the forward continuous division of cell cycle G1 / S and reduce the DNA synthesis, and methyl selenocysteine in selenium can reduce the activity of cell circulating protein kinase edk2, so that cell differentiation cannot pass through the S phase, thereby inhibiting the cell growth. After the viable bacterium-bifidobacterium is applied to a human body, the viable bacterium-bifidobacterium can balance in-vivo flora imbalance, such as oncogenes generated under the conditions of excessive antibiotics, radiotherapy, chemotherapy or emotion depression and the like, has the effects of assisting the human body in absorbing selenium and balancing human flora, and has a crucial effect on selenium absorption of the human body.

Description

Technical Field

[0001] The invention relates to the technical field of cancer cell inhibition and treatment, and in particular to a live bacteria super-selenium composition capable of inhibiting the growth and division of cancer cells. Background Art

[0002] Selenium has many effects and benefits on tumors, including anti-oxidation, inhibiting tumor cell growth, enhancing immunity, reducing the side effects of chemotherapy drugs, and improving the quality of life. Selenium is a strong antioxidant that can remove free radicals in the body and reduce oxidative stress, thereby protecting cells from damage and reducing the occurrence and development of tumors. Selenium can inhibit the growth and reproduction of tumor cells and induce tumor cell apoptosis, thereby playing an anti-tumor role.

[0003] Selenium can enhance the body's immunity and improve the body's disease resistance, thus helping to prevent and treat tumors. Chemotherapy is one of the important means of treating tumors, but chemotherapy drugs often have certain side effects on the body. Selenium can reduce the side effects of chemotherapy drugs and alleviate the pain of patients.

[0004] Bifidobacterium is a type of anaerobic, inactive, non-spore-forming native flora that colonizes the intestines of humans and animals. Studies have shown that it has the ability to inhibit tumor cell proliferation, induce tumor cell apoptosis, stimulate the body's immune cells to secrete cytokines, and serve as a targeted vector for tumor gene therapy.

[0005] Based on this, a live bacteria super-selenium composition with the function of inhibiting the growth and division of cancer cells is proposed to achieve the inhibition of tumor cells. Summary of the invention

[0006] To achieve the above object, the present invention provides the following technical solution: a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells, wherein the live bacteria are bifidobacteria;

[0007] The bifidobacterium is one or more of Bifidobacterium breve, Bifidobacterium longum and Mycobacterium;

[0008] The Bifidobacterium breve is deposited in the Institute of Microbiology, Chinese Academy of Sciences, with the collection center number = AS 1.2213 = ATCC 15700, and is classified as Bifidobacterium breve;

[0009] The long bifidobacterium is deposited in JCM Y.Benno M-193, and the number of other collection centers is JCM 11347. It is classified and named as long bifidobacterium

[0010] The mycobacterium is deposited in Wuhan University with the original number XN10-17, and other depository centers have the number XN10-17, and the classification name is Mycobacterium sp.

[0011] Super-selenium is taken from Cardamine violeta, and super-selenium is prepared by obtaining an extract of Cardamine violeta.

[0012] A preparation method of a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells comprises the following steps:

[0013] The bifidobacterium is prepared into bacterial powder;

[0014] The bifidobacterium powder and super polyselenium are compounded.

[0015] Preferably, the preparation of the bifidobacterium powder is as follows: the bifidobacterium strain is inoculated into a liquid culture medium, cultured at 30-40°C for 10-30 hours, then inoculated into a large fermentation tank, and fermented and cultured at 35-40°C for 10-30 hours; the fermented strain and the fermentation liquid are separated to obtain bacterial mud, the bacterial mud is emulsified, and the emulsion is vacuum freeze-dried to obtain bacterial powder.

[0016] Preferably, the liquid culture medium comprises peptone, beef extract, yeast extract, dipotassium hydrogen phosphate, diammonium citrate, sodium acetate, glucose, Tween 80, magnesium sulfate, and manganese sulfate; the above ingredients are dissolved in distilled water, the pH value is adjusted to 6.2-6.4, and then high-pressure sterilization is performed.

[0017] Preferably, the bifidobacterium powder and super-polyselenium are compounded into a composition with a viable count of 5 billion / g, 15 billion / g, 25 billion / g, 50 billion / g or 100 billion / g.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention obtains a composition by compounding active bacteria and super-polyselenium. Selenium can inhibit the activity of pkc in tumor cells and inhibit the growth and proliferation of tumor cells. The inhibitory mechanism of selenium on tumors is to induce changes in cell cycle proteins, prevent the cell cycle G1 / S from continuing to divide, and reduce DNA synthesis. Methyl selenocysteine ​​in selenium can lead to a decrease in the activity of cell cycle protein kinase edk2, so that cell differentiation cannot pass through the S phase, thereby causing cell growth inhibition. The application of live bacteria-bifidobacterium can balance the imbalance of bacterial flora in the body after being taken by the human body, such as oncogenes produced when the human body takes excessive antibiotics, radiotherapy, chemotherapy or emotional depression, and has the function of assisting the human body in absorbing selenium and balancing the human flora, which plays a vital role in the human body's absorption of selenium. DETAILED DESCRIPTION

[0020] The present invention will be described in more detail below by way of examples, which are merely illustrative and do not in any way limit the scope of the present invention.

[0021] Example 1

[0022] The present invention provides a technical solution: a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells, wherein the live bacteria are bifidobacteria;

[0023] The bifidobacterium is one or more of Bifidobacterium breve, Bifidobacterium longum and Mycobacterium;

[0024] The Bifidobacterium breve is deposited in the Institute of Microbiology, Chinese Academy of Sciences, with the collection center number = AS 1.2213 = ATCC 15700, and the classification name is Bifidobacterium breve;

[0025] The long bifidobacterium is deposited in JCM Y.Benno M-193, and the number of other collection centers is JCM 11347. It is classified and named as long bifidobacterium

[0026] The mycobacterium is deposited in Wuhan University with the original number XN10-17, and other depository centers have the number XN10-17, and the classification name is Mycobacterium sp.

[0027] Super-selenium is taken from Cardamine violeta, and super-selenium is prepared by obtaining an extract of Cardamine violeta.

[0028] Example 2

[0029] The present invention provides a method for preparing a live bacteria super-selenium composition, comprising the following steps:

[0030] The bifidobacterium is prepared into bacterial powder;

[0031] The preparation of the bifidobacterium powder is as follows: inoculating the bifidobacterium strain into a liquid culture medium, culturing at 30-40° C. for 1-30 hours, then inoculating into a large fermentation tank, and continuing to ferment and culture at 35-40° C. for 1-30 hours; separating the fermented strain from the fermentation liquid to obtain bacterial mud, emulsifying the bacterial mud, and vacuum freeze-drying the emulsion to obtain bacterial powder;

[0032] The liquid culture medium comprises peptone, beef extract, yeast extract, dipotassium hydrogen phosphate, diammonium citrate, sodium acetate, glucose, Tween 80, magnesium sulfate and manganese sulfate; the above components are dissolved in distilled water, the pH value is adjusted to 6.2-6.4, and then high pressure sterilization is performed;

[0033] Compounding Bifidobacterium powder and super polyselenium;

[0034] The bifidobacterium powder and super-polyselenium are compounded into a composition with a viable count of 5 billion / g, 15 billion / g, 25 billion / g, 50 billion / g or 100 billion / g.

[0035] The present invention obtains a composition by compounding active bacteria and super-polyselenium. Selenium can inhibit the activity of pkc in tumor cells and inhibit the growth and proliferation of tumor cells. The inhibitory mechanism of selenium on tumors is to induce changes in cell cycle proteins, prevent the cell cycle G1 / S from continuing to divide, and reduce DNA synthesis. Methyl selenocysteine ​​in selenium can lead to a decrease in the activity of cell cycle protein kinase edk2, so that cell differentiation cannot pass through the S phase, thereby causing cell growth inhibition. The application of live bacteria-bifidobacterium can balance the imbalance of bacterial flora in the body after being taken by the human body, such as oncogenes produced when the human body takes excessive antibiotics, radiotherapy, chemotherapy or emotional depression, and has the function of assisting the human body in absorbing selenium and balancing the human flora, which plays a vital role in the human body's absorption of selenium.

[0036] Example 3

[0037] Mechanisms of Selenium in Fighting Carcinogenesis:

[0038] (1) Evidence of antioxidant activity: The anticancer effect of selenium is mediated through the antioxidant activity of its component GPxs, and its theoretical basis is that reactive oxygen species (ROS) can cause cancer.

[0039] (2) Improving immune surveillance mechanisms: Selenium can promote immune surveillance to fight cancer cell metastasis. This theory is supported because selenium deficiency can lead to immunosuppression, and when selenium is taken as a chemical prevention, some immune indicators can be restored and improved. The important basic principles (functions) of selenium on human health are mainly through the effects of thyroid hormone metabolism, antioxidant defense system and immune function.

[0040] (3) Changing the metabolism of carcinogens: Selenium can restrict carcinogens-DNA and affect its metabolic process. This effect is expected to interrupt the early stages of cancer formation.

[0041] (4) Inhibit cancer proliferation and promote apoptosis: Selenium inhibits the growth and development of cancer cells and interferes with their spread. Some scholars have proven that different selenium compounds have pro-apoptotic ativities on cancer cells. These cancer cell growth inhibition and apoptotic effects are caused by the special metabolites of selenium entering the cancer tissue through different pathways.

[0042] (5) Inhibition of angiogenesis: Microvessels in tumors or growing lesions are inhibited by selenium. Its effect may be mediated by the inhibition of endothelial matrix metalloproteinases (MMPs) and angiogenic mediators, vascular endothelial growth factor. When the intake of selenium reaches the chemoprevention level, selenium induces the inhibition of breast cancer angiogenesis. Increasing selenium (selenium-rich garlic, sodium selenite) leads to a significant decrease in microvessel density in breast cancer tumors. The inhibition of related angiogenesis may be a new mechanism of selenium's anti-cancer activity in vivo, and the mediation of anti-vascular activity may involve multiple mechanisms.

[0043] Example 4

[0044] Effects of Active Bacteria-Bifidobacterium on Gastrointestinal Absorption

[0045] Promote digestive health: Bifidobacterium multiplies in the human body and occupies the intestines, which can inhibit the growth of harmful bacteria, maintain the balance of intestinal flora, and prevent intestinal infections. In addition, it can also secrete substances that make intestinal peristalsis smooth and promote the digestion and absorption of food.

[0046] Enhance immunity: Bifidobacterium can stimulate the body to produce immunoglobulins and antibodies, improve the body's immunity and prevent infection. In addition, it can also bind to intestinal epithelial cells, increase the integrity of the intestinal wall, prevent harmful substances from entering the blood circulation, and improve the body's defense against external pathogenic microorganisms.

[0047] Promote nutrient absorption: Bifidobacteria can break down and digest the difficult-to-absorb parts of food in the intestines and convert them into easily absorbed substances, thereby improving the utilization efficiency of food and increasing the body's absorption of nutrients.

[0048] Regulate emotional stability: The intestine is called the "second brain", and bifidobacteria communicate with the brain through the gut-brain axis. Studies have shown that bifidobacteria can regulate emotional stability and relieve psychological problems such as anxiety and depression by producing neurotransmitters and metabolites.

[0049] The effect of active bacteria-Bifidobacteria on inhibiting tumor cells:

[0050] Even when the tumor has abundant angiogenesis, the solid tumor area still has a hypoxic environment. The oxygen partial pressure of healthy tissue is 3% to 5% (2.67 to 13.3 kPa1), while the oxygen partial pressure of the hypoxic area of ​​the solid tumor is less than 1% (0 to 2.67 kPa).

[0051] The reason is that the growth rate of the tumor exceeds the rate of its blood vessel generation, and the blood vessels formed in the solid tumor area are not enough to provide the oxygen required by the entire tumor. Although tumor cells induce the production of vascular endothelial growth factor by secreting hypoxia-inducible factor and cytokines, and then recruit endothelial cells from healthy blood vessels around the tumor or endothelial progenitor cells in the bone marrow to the tumor tissue to form a new vascular system; however, most of these blood vessels are immature, prone to branching and intravascular branching, incomplete vascular walls, closed vascular ends, and prone to necrosis, so the new blood vessels in the tumor are constantly regenerating and disappearing.

[0052] It is precisely because the tumor grows too fast and the vascular system formed in the tumor area is unstable and immature that it cannot provide sufficient fresh blood to the central area of ​​the tumor, which leads to the formation of the tumor hypoxic zone. The hypoxic area of ​​the tumor will not only affect the treatment of the tumor and reduce the tumor programmed cell death rate, but also reduce the sensitivity of tumor cells to radiotherapy and chemotherapy, making it a forbidden zone for tumor treatment. In addition, hypoxia may also enhance the invasion and metastasis ability of tumor cells and promote the growth and metastasis of tumor cells. Although hypoxia has the above-mentioned disadvantages for tumor treatment, the hypoxic zone can become an emerging target for anti-tumor treatment. That is, the characteristic of bifidobacteria that can colonize in the hypoxic area of ​​solid tumors makes bifidobacteria the best candidate carrier for anti-tumor molecular targeted therapy.

[0053] After bifidobacterium enters the human body, its DNA can activate dendritic cells and T cells, causing them to be produced in large quantities. The large-scale production of these cells, factors, etc. can enhance the body's immune function, protect the body, and inhibit the growth of tumor cells.

[0054] Example 5

[0055] Experiments on the inhibition of selenium on tumor cells:

[0056] Professor Clark of the University of Arizona Cancer Center conducted a 13-year double-blind intervention trial of selenium supplementation in the United States. This experiment met strict scientific standards and was conducted in 7 clinical centers. The results showed that: daily supplementation of 200 micrograms of selenium reduced cancer mortality by 50% and the total incidence of cancer by 37%. Among them, the incidence of 3 types of cancer decreased most significantly: prostate cancer decreased by 63%, colorectal cancer decreased by 58%, and lung cancer decreased by 46%.

[0057] From 1986 to 1994, Professor Yu Shuyu conducted an experiment on selenium supplementation to prevent liver cancer among 130,000 residents in Qidong County, Jiangsu Province (a high-incidence area of ​​liver cancer in China). The experiment showed that after eight years of experiments, the blood selenium content of residents in the high-incidence area of ​​liver cancer was significantly lower than that in the low-incidence area of ​​liver cancer. Selenium supplementation can reduce the incidence of hepatitis by 35% and the incidence rate of those with a family history of liver cancer by 50%. In addition, it was observed that selenium supplementation can enhance human immunity and resist the spread of viral hepatitis in the population.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A live bacteria super-selenium composition for inhibiting the growth and division of cancer cells, characterized in that: The live bacteria are bifidobacteria; The bifidobacterium is one or more of Bifidobacterium breve, Bifidobacterium longum and Mycobacterium; The Bifidobacterium breve is deposited in the Institute of Microbiology, Chinese Academy of Sciences, with the collection center number = AS 1.2213 = ATCC 15700, and is classified as Bifidobacterium breve; The long bifidobacterium is deposited in JCM Y.Benno M-193, and the number of other collection centers is JCM 11347. It is classified and named as long bifidobacterium The mycobacterium is deposited in Wuhan University with the original number XN10-17, and other depository centers have the number XN10-17, and the classification name is Mycobacterium sp. Super-selenium is taken from Cardamine violeta, and super-selenium is prepared by obtaining an extract of Cardamine violeta.

2. A preparation of a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells, according to claim 1, characterized in that: The following steps are involved: The bifidobacterium is prepared into bacterial powder; The bifidobacterium powder and super polyselenium are compounded.

3. The preparation of a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells according to claim 2, characterized in that: The preparation of the bifidobacterium powder is as follows: inoculating the bifidobacterium strain into a liquid culture medium, culturing at 30-40°C for 10-30 hours, then inoculating into a large fermentation tank, and continuing to ferment and culture at 35-40°C for 10-30 hours; separating the fermented strain from the fermentation liquid to obtain bacterial mud, emulsifying the bacterial mud, and vacuum freeze-drying the emulsion to obtain bacterial powder.

4. The preparation of a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells according to claim 2, characterized in that: The liquid culture medium comprises peptone, beef extract, yeast extract, dipotassium hydrogen phosphate, diammonium citrate, sodium acetate, glucose, Tween 80, magnesium sulfate, and manganese sulfate; The above ingredients were dissolved in distilled water, the pH was adjusted to 6.2-6.4, and then sterilized by high pressure.

5. The preparation of a live bacteria super-selenium composition for inhibiting the growth and division of cancer cells according to claim 2, characterized in that: The bifidobacterium powder and super polyselenium are compounded into a composition with a live bacteria count of 5 billion / g, 15 billion / g, 25 billion / g, 50 billion / g or 100 billion / g.