Recombinant collagen-containing firming and anti-wrinkle composition and application thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a firming and anti-wrinkle composition containing recombinant collagen and various ginseng extracts, which provides anti-aging and firming effects and can greatly reduce the cost of products by compounding one or more extracts of different parts of ginseng with III-type recombinant collagen.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a firming and anti-wrinkle composition containing recombinant collagen and multiple ginseng extracts and applications thereof. Background Art

[0002] Collagen is a biological macromolecule and the main component of animal connective tissue. It is also the most abundant and widely distributed functional protein in mammals, accounting for 25%-30% of the total protein, and even as high as 80% or more in some organisms. Its sources include animal sources and biosynthesis.

[0003] Recombinant collagen refers to the use of recombinant DNA technology to genetically manipulate and modify the gene encoding the desired human collagen protein, using plasmids or viral vectors to bring the target gene into appropriate host cells (cells, yeast or other eukaryotic cells, etc.), expressing and translating it into collagen or collagen-like polypeptides, and then preparing it through extraction and purification steps.

[0004] From the perspective of the industrial chain, the upstream of the recombinant collagen industry chain is the supplier of collagen production raw materials, among which the raw materials of recombinant collagen mainly come from Pichia pastoris and Escherichia coli produced by microbial fermentation enterprises. The midstream is the preparation and processing of recombinant collagen, among which the fixed costs such as production equipment of recombinant collagen manufacturers are relatively high, and the variable costs such as raw materials are relatively low. At the same time, the profits of midstream manufacturers are relatively high. The downstream is the terminal application of recombinant collagen products, involving medical health, skin care, food and other fields. The biggest disadvantage of recombinant collagen at present is that it is expensive. For example, the price of 5% recombinant collagen reaches 98,000 / kg, which makes the application of the product limited by cost. Therefore, it is urgent to prepare a low-cost composition that can achieve the same technical effect as recombinant collagen. Summary of the invention

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a firming and anti-wrinkle composition containing recombinant collagen and multiple ginseng extracts. By using one or more extracts from different parts of ginseng in combination with type III recombinant collagen, it provides anti-aging and firming effects and can greatly reduce the cost of the product.

[0006] Technical Solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The invention provides a firming and anti-wrinkle composition containing recombinant collagen and multiple ginseng extracts, which comprises recombinant collagen and ginseng extracts; the ginseng extracts are at least one or more extracts of ginseng roots, flowers, stems and leaves.

[0009] Furthermore, the ginseng root extract, ginseng flower extract and ginseng stem and leaf extract are all prepared by crushing and water extraction.

[0010] Further, recombinant collagen: type III recombinant collagen (type A) or type XVII recombinant collagen (type A) is used. The optional manufacturer of type III recombinant collagen (type A) is: Jinbo Biotechnology; the trade name is: micoreCol.III-CP5-SMART.

[0011] Further, the ginseng extract includes at least one or two of ginseng stem / leaf / flower extract and ginseng root extract. The ginseng stem / leaf / flower extract includes ginseng stem and leaf extract and ginseng flower extract, and the weight ratio of ginseng stem and leaf extract to ginseng flower extract is 1-5:1; preferably, the weight ratio of ginseng stem and leaf extract to ginseng flower extract is 3:1.

[0012] Further, the ginseng stem / leaf / flower extract is prepared as follows:

[0013] (1) Grinding: Grinding the ginseng stems / leaves and ginseng flowers separately, and sieving through a 10-100 mesh sieve; weighing the ginseng stems / leaves and ginseng flowers separately at a weight ratio of 1-5:1, and then mixing them;

[0014] (2) Soaking: Based on the weight of the mixture, soak it in 10-20 times the weight of deionized water and 1-5 times the weight of 1,3-butanediol for 3-8 hours;

[0015] (3) Extraction: Boil at 95-100°C with stirring for 4-5 hours, and filter and separate using a 200-300 mesh filter;

[0016] (4) Concentration: Concentrate by distillation under reduced pressure at 35-85°C to 1 / 8-1 / 12 of the original volume;

[0017] (5) Decolorization: Based on the weight after concentration, add 5-20% of activated carbon for decolorization, add 0.3-0.8% of p-hydroxyacetophenone and 0.3-0.8% of 1,2-hexanediol for preparation, place at low temperature 6-8°C for 48 hours, and then filter and fill.

[0018] Furthermore, the ginseng root extract is prepared by the following method:

[0019] (1) Weigh 5-10 parts of ginseng root powder by weight, add 60-80 parts of water and 10-20 parts of ethanol, and extract by stirring and ultrasonic heating (75-80° C., 6 h);

[0020] (2) After concentrating and drying under reduced pressure at 35-85° C., a powder is obtained, and then 20-40 parts of butanediol and 50-80 parts of water are added by weight to dissolve;

[0021] (3) Use 5-20 parts by weight of activated carbon for decolorization and deodorization, let stand at low temperature (6-8°C, 48h), filter and fill.

[0022] Further, the volume percentage concentration ratio of the ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen is 1:0.25:0.01-0.5. Specifically, the volume percentage concentration ratio of the ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen can be 1:0.25:0.01, 1:0.25:0.02, 1:0.25:0.03, 1:0.25:0.04, 1:0.25:0.05, 1:0.25:0.06, 1:0.25:0.07, 1:0.25:0.08, 1:0.25:0.09, 1:0.25:0.1, 1:0.25:0.2, 1:0.25:0.3, 1:0.25:0.4, 1:0.25:0.5.

[0023] Furthermore, the volume percentage concentration ratio of the ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen is 1:0.25:0.05-0.1.

[0024] Furthermore, the composition increases the expression of COL1A1 gene, COL3A1 gene and Elastin gene in human fibroblasts.

[0025] Preferably, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is 1:0.25:0.1-0.5, which can increase the expression of COL1A1 gene in human fibroblasts. Specifically, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition can be any value of 1:0.25:0.1, 1:0.25:0.2, 1:0.25:0.3, 1:0.25:0.4, 1:0.25:0.5 and the range between any two values.

[0026] Preferably, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is 1:0.25:0.02-0.1, which can increase the expression of COL3A1 gene in human fibroblasts. Specifically, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition can be any value of 1:0.25:0.02, 1:0.25:0.03, 1:0.25:0.04, 1:0.25:0.05, 1:0.25:0.06, 1:0.25:0.07, 1:0.25:0.08, 1:0.25:0.09, 1:0.25:0.1 and the range between any two values.

[0027] Most preferably, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is 1:0.25:0.05, which can increase the expression of COL3A1 gene in human fibroblasts.

[0028] Preferably, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is 1:0.25:0.05-0.1, which can increase the expression of Elastin gene in human fibroblasts. Specifically, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is any value of 1:0.25:0.05, 1:0.25:0.06, 1:0.25:0.07, 1:0.25:0.08, 1:0.25:0.09, 1:0.25:0.1 and the range between any two values.

[0029] Furthermore, the composition can inhibit the expression of MMP1 gene in human fibroblasts.

[0030] Preferably, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition is 1:0.25:0.02-0.1, which can inhibit the expression of MMP1 gene in human fibroblasts. Specifically, the volume percentage concentration ratio of ginseng stem / leaf / flower extract, ginseng root extract and recombinant collagen in the composition can be any value of 1:0.25:0.02, 1:0.25:0.03, 1:0.25:0.04, 1:0.25:0.05, 1:0.25:0.06, 1:0.25:0.07, 1:0.25:0.08, 1:0.25:0.09, 1:0.25:0.1 and the range between any two values.

[0031] The invention discloses a firming and anti-wrinkle composition containing recombinant collagen and various ginseng extracts, which can be used in cosmetics and skin care products.

[0032] Beneficial Effects

[0033] Compared with the known public technology, the technical solution provided by the present invention has the following beneficial effects:

[0034] The present invention studies multiple functions. In addition to directly supplementing collagen, it can also stimulate the synthesis of collagen (collagen-1,3), elastin, and reduce the expression of matrix metalloproteinases, thereby reducing the reduction of collagen. Ginseng has a synergistic effect with type III collagen, which can reduce the amount of collagen used and save costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0036] Figure 1 The COL1A1 fluorescence quantitative PCR test results of the present invention;

[0037] Figure 2 The COL3A1 fluorescence quantitative PCR test results of the present invention;

[0038] Figure 3 This is the MMP1 fluorescence quantitative PCR detection result of the present invention;

[0039] Figure 4 This is the Elastin fluorescence quantitative PCR detection result of the present invention. DETAILED DESCRIPTION

[0040] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] The present invention will be further described below in conjunction with the embodiments.

[0042] The specific composition and preparation of each raw material in the embodiment are as follows:

[0043] Raw material 1: ginseng stem / leaf / flower extract, the preparation method of which is as follows:

[0044] (1) Grinding: Grinding the ginseng stems and leaves and ginseng flowers separately, and sieving through a 50-mesh sieve, wherein the weight ratio of the ginseng stems and leaves to the ginseng flowers is 3:1;

[0045] (2) Soaking: Based on the weight of the mixture, soak in 15 times the weight of deionized water and 3.5 times the weight of 1,3-butanediol for 5 hours;

[0046] (3) Extraction: Boil at 95-100°C with stirring for 4.5 hours, and filter through a 250-mesh filter;

[0047] (4) Concentration: Concentrate by vacuum distillation at 70°C to 1 / 10 of the original volume;

[0048] (5) Based on the weight after concentration, 10% of activated carbon is added for decolorization, and then 0.5% of p-hydroxyacetophenone and 0.5% of 1,2-hexanediol are added for mixing, and the mixture is filtered and filled at low temperature (6-8° C., 48 hours).

[0049] Raw material 2: ginseng root extract, the preparation method of which is as follows:

[0050] (1) Weigh 8 parts of ginseng root powder by weight, add 70 parts of water and 15 parts of ethanol, and extract by stirring and ultrasonic heating (75-80° C., 6 h);

[0051] (2) After concentrating and drying under reduced pressure at 70°C, a powder is obtained, and then 30 parts of butanediol and 75 parts of water are added by weight to dissolve;

[0052] (3) Use 15 parts by weight of activated carbon for decolorization and deodorization, filter and fill at low temperature (6-8°C, 48h).

[0053] Raw material 3: Recombinant collagen: Type III recombinant collagen (type A) is used, manufacturer: Jinbo Bio, trade name: micoreCol.III-CP5-SMART.

[0054] (I) Embodiment

[0055] Example 1: Raw material 1 (1%)

[0056] Take 100 μL of raw material 1, add it to 9.9 mL of serum-free DMEM medium, mix well, prepare a 1% raw material 1 solution, and set aside.

[0057] Example 2: Raw material 2 (0.25%)

[0058] Take 25 μL of raw material 2, add it to 9.975 mL of serum-free DMEM medium, mix well, and prepare a 0.25% raw material 2 solution for use.

[0059] Example 3: Raw material 3 (0.1%)

[0060] Take 0.01 g of raw material 3, add it to 10 mL of serum-free DMEM medium, mix well, and prepare a 0.1% raw material 3 solution for use.

[0061] Example 4: Raw material 3 (0.05%)

[0062] Take 5 mL of 0.1% raw material 3 solution, add it to 5 mL of serum-free DMEM medium, mix well, prepare 0.05% raw material 3 solution, and set aside.

[0063] Example 5: Raw material 3 (0.02%)

[0064] Take 2 mL of 0.1% raw material 3 solution, add it to 8 mL of serum-free DMEM medium, mix well, prepare 0.02% raw material 3 solution, and set aside.

[0065] Example 6: Raw material 3 (0.01%)

[0066] Take 1 mL of 0.1% raw material 3 solution, add it to 9 mL of serum-free DMEM medium, mix well, prepare 0.01% raw material 3 solution, and set aside.

[0067] Example 7: Raw material 1 (1%) + raw material 2 (0.25%)

[0068] Take 100 μL of raw material 1 and 25 μL of raw material 2, add them into 9.875 mL of serum-free DMEM medium, mix well, prepare a raw material 1 (1%) + raw material 2 (0.25%) solution, and set aside.

[0069] Example 8: Raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.1%)

[0070] Take 100 μL of raw material 1 and 25 μL of raw material 2, add them to 9.875 mL of 0.1% raw material 3 solution, mix well, and prepare a solution of raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.1%) for use.

[0071] Example 9: Raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.05%)

[0072] Take 100 μL of raw material 1 and 25 μL of raw material 2, add them into 9.875 mL of 0.05% raw material 3 solution, mix well, prepare raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.05%) solution, and set aside.

[0073] Example 10: Raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.02%)

[0074] Take 100 μL of raw material 1 and 25 μL of raw material 2, add them into 9.875 mL of 0.02% raw material 3 solution and mix evenly to prepare a solution of raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.02%) for use.

[0075] Example 11: Raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.01%)

[0076] Take 100 μL of raw material 1 and 25 uI of raw material 2, add them to 9.875 mL of 0.01% raw material 3 solution, mix well, and prepare a solution of raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.01%) for use.

[0077] The manufacturer of the DMEM culture medium in Example 1-11 is Gibco, and the model is high-glucose type.

[0078] (II) Performance test

[0079] (1) Detection indicators

[0080] The present invention is based on primary fibroblasts (Fbs) derived from human skin, and evaluates the anti-wrinkle and firming effects of the samples from the aspects of COL1A1, COL3A1, Elastin, and MMP1 gene expression. Among them, the manufacturer of primary fibroblasts derived from human skin is Genentech. The manufacturer of TGF-β1 is PeproTech, and the model is AF-100-21C-100;

[0081] Type I collagen: Type I collagen (COL1A1) is the most abundant protein in the human body, accounting for more than 80% of total collagen, and is essential for the function and stability of ECM (especially skin). Therefore, the anti-wrinkle and firming effect of the sample can be evaluated by detecting the expression of collagen in cells after the sample is treated.

[0082] Type III collagen: Type III collagen (COL3A1) is an important extracellular matrix component in the skin, which is mainly synthesized and secreted by fibroblasts in the skin. Skin aging caused by ultraviolet radiation and other reasons will lead to a decrease in type III collagen in the skin, causing skin sagging and wrinkles.

[0083] Elastin: Elastin is essential for maintaining the elasticity, flexibility and overall appearance of the skin. It is mainly concentrated in the dermis and intertwined with the collagen network to provide structural support for the skin. UV exposure accelerates the degradation of elastin, which is a major factor in skin aging and photoaging.

[0084] Matrix metalloproteinase 1 (MMP1): Also known as collagenase 1, it is an important enzyme that plays a role in the skin. UV exposure can activate MMP1, which leads to excessive degradation of collagen and elastin, causing aging symptoms such as wrinkles and loss of elasticity in the skin.

[0085] Transforming growth factor β1 (TGF-β1): TGF-β1 is a multifunctional cytokine that can increase the synthesis of collagen and elastin in fibroblasts. It can reduce the degradation of collagen and elastin by inhibiting the activity of MMPs, thus helping to maintain skin elasticity and reduce wrinkles.

[0086] The main instruments and reagents required for the experiment are shown in the following table:

[0087]

[0088] (2) Anti-wrinkle and firming efficacy testing

[0089] Fibroblasts were seeded in 24-well plates and cultured under saturated humidity, 5% CO2, and 37°C. The next day, samples were added as described in Table 1. RNA was collected from each group 24 hours after addition, and fluorescence quantitative PCR was performed to detect the expression of COL1A1, COL3A1, Elastin, and MMP1 genes.

[0090] Table 1 Anti-wrinkle and firming efficacy experimental grouping

[0091]

[0092] All data were expressed as mean ± standard deviation. The groups were compared using t-test. P < 0.05 was considered to be significantly different, and P < 0.01 was considered to be extremely significantly different.

[0093] Table 2 RQ values ​​of COL1A1 and COL3A1 genes (vs UV irradiation group)

[0094]

[0095] #: Compared with the blank control group, P < 0.05; ##: Compared with the blank control group, P < 0.01 *: Compared with the UV irradiation group, P < 0.05; **: Compared with the UV irradiation group, P < 0.01

[0096] Table 3 RQ values ​​of MMP1 and Elastin genes (vs UV irradiation group)

[0097]

[0098] Note: Compared with the blank control group, P<0.05; ##: Compared with the blank control group, P<0.01; *: Compared with the UV irradiation group, P<0.05; **: Compared with the UV irradiation group, P<0.01

[0099] The in vitro evaluation system for anti-wrinkle and firming efficacy of the present invention is mainly carried out from the expression of COL1A1, COL3A1, MMP1 and Elastin genes in human fibroblasts. Compared with the UV irradiation group, the results show that:

[0100] 1) Raw material 1 (1%), raw material 2 (0.25%), raw material 3 (0.1%), raw material 3 (0.05%), raw material 3 (0.02%), raw material 3 (0.01%), raw material 1 (1%) + raw material 2 (0.25%), raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.1%), raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.05%), raw material 1 (1%) + raw material 2 (0 .25%) + raw material 3 (0.02%), raw material 1 (1%) + raw material 2 (0.25%) + raw material 3 (0.01%), can increase the expression of COL1A1 gene in human fibroblasts, and the up-regulation amounts are 14% ± 5%, 12% ± 3%, 31% ± 5%, 26% ± 7%, 14% ± 4%, 11% ± 1%, 23% ± 8%, 74% ± 14%, 51% ± 15%, 43% ± 8%, 34% ± 14%, respectively;

[0101] 2) All experimental groups could increase the expression of COL3A1 gene in human fibroblasts, and the upregulation amounts were 11%±2%, 15%±5%, 30%±12%, 35%±8%, 31%±11%, 26%±5%, 20%±8%, 74%±17%, 79%±20%, 77%±4%, and 58%±6%, respectively;

[0102] 3) All experimental groups could inhibit the expression of MMP1 gene in human fibroblasts, and the downregulation amounts were 33%±4%, 11%±2%, 40%±2%, 36%±4%, 40%±1%, 26%±5%, 37%±6%, 49%±2%, 44%±5%, 49%±2%, and 45%±8%, respectively;

[0103] 4) Except raw material 1 (1%), other samples can increase the expression of Elastin gene in human fibroblasts, and the up-regulation amounts are 14%±1%, 35%±5%, 25%±4%, 16%±3%, 17%±1%, 12%±1%, 41%±12%, 34%±10%, 15%±4%, and 12%±2%, respectively.

[0104] In summary, ginseng stem / leaf / flower extract, ginseng root extract, recombinant collagen and their mixed solutions can increase the expression of COL1A1 and COL3A1 genes in human fibroblasts, inhibit the expression of MMP1 gene in human fibroblasts, and increase the expression of Elastin gene in human fibroblasts (except raw material 1 (1%)), thereby exerting anti-wrinkle and firming effects.

[0105] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A firming and anti-wrinkle composition containing recombinant collagen, characterized in that: The invention comprises recombinant collagen and ginseng extract; the ginseng extract is an extract of at least one or more of ginseng roots, flowers, stems and leaves.

2. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The ginseng root extract, ginseng flower extract and ginseng stem and leaf extract are all prepared by crushing and water extraction.

3. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The ginseng extract includes at least one or two of a ginseng stem / leaf / flower extract and a ginseng root extract.

4. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The volume percentage concentration ratio of the ginseng stem / leaf / flower extract, the ginseng root extract and the recombinant collagen is 1:0.25:0.01-0.

5.

5. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The volume percentage concentration ratio of the ginseng stem / leaf / flower extract, the ginseng root extract and the recombinant collagen is 1:0.25:0.05-0.

1.

6. The firming and anti-wrinkle composition containing recombinant collagen according to claim 3, characterized in that: The preparation method of the ginseng stem / leaf / flower extract is as follows: (1) Grinding: Grinding the ginseng stems / leaves and ginseng flowers separately, and sieving through a 10-100 mesh sieve; weighing the ginseng stems / leaves and ginseng flowers separately at a weight ratio of 1-5:1, and then mixing them; (2) Soaking: Based on the weight of the mixture, soak it in 10-20 times the weight of deionized water and 1-5 times the weight of 1,3-butanediol for 3-8 hours; (3) Extraction: Boil at 95-100°C with stirring for 4-5 hours, and filter and separate using a 200-300 mesh filter; (4) Concentration: Concentration by vacuum distillation at 35-85°C; (5) Decolorization: Based on the weight after concentration, add 5-20% of activated carbon for decolorization, add 0.3-0.8% of p-hydroxyacetophenone and 0.3-0.8% of 1,2-hexanediol for preparation, place at low temperature 6-8°C for 48 hours, and then filter and fill.

7. The firming and anti-wrinkle composition containing recombinant collagen according to claim 3, characterized in that: The preparation method of the ginseng root extract is as follows: (1) Weigh 5-10 parts of ginseng root powder by weight, add 60-80 parts of water and 10-20 parts of ethanol, stir ultrasonically, heat at 75-80° C. for 6 hours, and then extract; (2) concentrating and drying under reduced pressure at 35-85° C., adding 20-40 parts of butanediol and 50-80 parts of water by weight to dissolve; (3) Use 5-20 parts by weight of activated carbon for decolorization and deodorization, let stand at low temperature, filter and fill.

8. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The composition increases the expression of COL1A1 gene, COL3A1 gene and Elastin gene in human fibroblasts.

9. The firming and anti-wrinkle composition containing recombinant collagen according to claim 1, characterized in that: The composition can inhibit the expression of MMP1 gene in human fibroblasts.

10. A firming and anti-wrinkle composition containing recombinant collagen as claimed in any one of claims 1 to 9, which can be used in cosmetics and skin care products.

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