Device and method for researching metabolism of pesticide in plant

Abstract

The invention discloses an in-plant pesticide metabolism research device and method, and belongs to the technical field of crop pesticide residue research. The research cabinet is provided with a rotary supporting assembly, the movable end of the rotary supporting assembly is detachably connected with a cultivation box, a root system research box is placed on the lower side of the cultivation box, and the upper side of the cultivation box is connected with a shielding cover; the inner cavities of the cultivation box, the root system research box and the shielding cover are equally divided into four areas; by means of the mode, the direction simulation assembly, the sunlight track simulation assembly, the fluorescent lamp and the laminate are matched to achieve the simulation effect on real sunlight, and the two concentration control assemblies are used for controlling the pesticide amount and the water amount respectively to adjust the pesticide concentration; the liquid spraying assembly is used for spraying the blended liquid into a single area of the cultivation box, and the cultivation box is rotated through the rotary supporting assembly to spray pesticides with different concentrations into different areas in the cultivation box, so that crops in each area are a control group; the influence of the pesticide concentration on the pesticide metabolism capability of plants is researched.

Description

Technical Field

[0001] The invention relates to the technical field of crop pesticide residue research, and in particular to a device and method for studying pesticide metabolism in plants. Background Art

[0002] Pesticides are widely used in agricultural production to prevent and control pests, weeds and other harmful organisms. However, if used improperly or excessively, pesticide residues may remain in agricultural products, affecting food safety and human health. The amount of pesticide used, the frequency of application, the method of application, and climatic factors such as temperature, humidity, and rainfall will directly affect the residue level.

[0003] Chinese patent CN207095934U discloses a radioactive test application device specifically used for pesticide plant metabolism research, including an injection pump capable of setting the flow rate of radioactive labeled pesticides, a syringe, and a sealing device. The device performs a radioactive labeled pesticide spray application test by eluting the radioactive labeled drug adhered to the inner wall surface of the sealing cover and merging it with the droplets in the original collecting tank. However, the device cannot simulate the actual growth environment of crops in the farmland, and it is difficult to conveniently conduct control experiments to more accurately evaluate the effects of variables. The reliability and validity of the research results are insufficient.

[0004] Based on this, the present invention designs a device and method for studying pesticide metabolism in plants to solve the above problems. Summary of the invention

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a device and method for studying pesticide metabolism in plants.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] An apparatus for studying pesticide metabolism in plants, including a research cabinet;

[0008] A rotating support assembly is provided at the bottom of the research cabinet, and a movable end of the rotating support assembly is detachably connected to a cultivation box, a root system research box is placed at the lower side of the cultivation box, and a shielding cover is connected to the upper side of the cultivation box through a card block and a card slot structure;

[0009] A "cross" shaped partition is arranged inside the incubator, the root system research box and the shielding cover, so that the inner cavity of the incubator, the root system research box and the shielding cover is divided into four areas of equal volume; a wire mesh is arranged at the bottom of each area of ​​the incubator;

[0010] The inner wall of the research cabinet is fixed with a thermostat, and each area of ​​the shielding cover is equipped with a temperature and humidity sensor;

[0011] A shelf is fixedly installed at the upper end of the inner cavity of the research cabinet, and a daylight simulation mechanism is arranged on the shelf. The daylight simulation mechanism includes an azimuth simulation component, a daylight track simulation component and a fluorescent lamp. The azimuth simulation component is arranged at the upper end of the shelf, the daylight track simulation component is arranged at the moving end of the azimuth simulation component, and the fluorescent lamp is arranged at the moving end of the daylight track simulation component; the daylight track simulation component is used to control the fluorescent lamp to move along an arc track to simulate the sunrise and sunset of the sun, and the azimuth simulation component is used to control the fluorescent lamp to rotate around the incubator to simulate the change of the sunrise position of the sun in different months; the shelf adopts electrically controlled dimming glass;

[0012] The inner wall of the research cabinet is provided with a pesticide and water control mechanism, which includes a concentration control component and a liquid spray component, which are respectively arranged on the outer wall and the inner wall of the research cabinet; the concentration control component is provided with two groups for controlling the amount of pesticide and the amount of water, respectively, and the concentration of the pesticide is adjusted by changing the ratio of pesticide to water; the liquid spray component is used to spray the prepared liquid into a single area of ​​the incubator;

[0013] A sample collection mechanism is provided on the left outer wall of the research cabinet, which is used for sampling after removing the crops on the surface of the pesticide.

[0014] Furthermore, the orientation simulation component includes a first control motor, a worm, a turbine, a limit guide wheel and a universal ball. The first control motor is fixedly mounted on the upper end of the layer plate, and the output end of the first control motor is fixedly mounted with a worm; the outer side of the turbine is meshingly connected with the worm; a plurality of limit guide wheels are rotatably mounted on the upper end of the layer plate and rollingly connected with the inner side of the turbine; a plurality of universal ball rollers are also provided on the upper end of the layer plate and rollingly connected with the lower end of the turbine.

[0015] Furthermore, the sunlight track simulation component includes an arc frame, a slider and a traction component, the arc frame is fixedly installed on the inner side of the turbine, and the slider is connected to the arc frame in a limited sliding manner; the traction components for controlling the movement of the slider are symmetrically arranged on both sides of the arc frame;

[0016] The traction assembly includes a second control motor, a roller and a traction rope. The second control motor is fixedly connected to the turbine. A roller for winding the traction rope is fixedly installed at the output end of the second control motor. The two ends of the traction rope are respectively fixedly connected to the end of the slider and the roller.

[0017] A plurality of fluorescent lamps are fixedly mounted on the lower end of the slide block.

[0018] Furthermore, the rotating support assembly includes a rotating table, a support, a rotating motor, a driving guide wheel, a driven guide wheel, a receiving groove and a limit assembly. The support is fixedly mounted on the inner bottom of the research cabinet, and a ball is provided on the upper end of the support to support the rotatable rotating table; the rotating motor is fixedly mounted on the inner bottom of the research cabinet, and a driving guide wheel is fixedly mounted on the output end of the rotating motor; a plurality of driven guide wheels are rotatably mounted on the inner bottom of the research cabinet; the driving guide wheel and the driven guide wheel are both rollingly connected to the outer edge of the rotating table; a receiving groove is provided on the rotating table, and the inner wall of the receiving groove is limitedly slidably connected to the side wall of the incubator; and limit assemblies for locking the incubator in the receiving groove are also provided on both sides of the opening of the receiving groove on the rotating table.

[0019] Furthermore, the concentration control component includes a vertical servo motor, a screw rod, a sliding rod, a mounting frame, a vertical moving plate, a liquid storage barrel, a temporary storage frame and a first connecting pipe. The mounting frame is fixedly connected to the side wall of the research cabinet, the screw rod is rotatably connected to the mounting frame through a bearing, and the sliding rod is fixedly connected to the mounting frame; the vertical servo motor is fixedly connected to the mounting frame, and the output end of the vertical servo motor is fixedly connected to the screw rod; the vertical moving plate is threadedly connected to the screw rod through a threaded sleeve, and the vertical moving plate is slidably connected to the sliding rod; the liquid storage barrel is fixedly connected to the vertical moving plate, and the temporary storage frame is fixedly connected to the side wall of the research cabinet, and the lower end of the temporary storage frame is connected to the lower end of the liquid storage barrel through the first connecting pipe, so that the liquid storage barrel and the temporary storage frame constitute a communicating vessel structure; the liquid storage barrels of the two concentration control components are used to store pesticides and water, respectively.

[0020] Furthermore, the liquid spray assembly includes an avoidance cylinder, a mounting plate, a mixing cylinder, an atomizing nozzle, a push rod, a pushing cylinder and a second connecting pipe. The avoidance cylinder is fixedly connected to the outer wall of the research cabinet, and the output end of the avoidance cylinder passes through the side wall of the research cabinet and is fixedly connected to the mounting plate; the mixing cylinder and the pushing cylinder are fixedly connected to the mounting plate, and the lower end of the mixing cylinder is provided with an atomizing nozzle facing the incubator; the temporary storage frames of the two concentration control assemblies are respectively connected to the mixing cylinder through the second connecting pipe; the output end of the pushing cylinder is fixedly installed with a push rod plugged into the mixing cylinder.

[0021] Furthermore, the sample collection mechanism includes a cleaning component and a sampling component, both of which are installed on the left side wall of the research cabinet. The cleaning component is used to clean the pesticides on the surface of the crops, and the sampling component is used to sample the crops after cleaning.

[0022] Furthermore, the cleaning component includes a rotating cleaning barrel, a bracket, a slot, a connecting rod, a servo push cylinder and a rotating drive component. The bracket is fixedly connected to the side wall of the research cabinet, and the rotating cleaning barrel is rotatably connected to the bracket; a hollow cavity is provided between the inner layer and the outer layer of the rotating cleaning barrel, and a plurality of water leakage holes are evenly provided on the inner layer of the rotating cleaning barrel; the servo push cylinder is fixedly connected to the side wall of the research cabinet, the output end of the servo push cylinder is rotatably connected to the lower end of the connecting rod through a bearing, and the upper end of the connecting rod is connected to the bottom of the rotating cleaning barrel through a key, so that the connecting rod can move vertically when it rotates synchronously with the rotating cleaning barrel; the upper end of the connecting rod extends into the inner layer of the rotating cleaning barrel and is fixedly connected to the bottom plate; the side wall of the rotating cleaning barrel is provided with a rotating drive component.

[0023] Furthermore, the sampling assembly includes a sampling rack, a material storage trough, a card slot, a through hole, a punch rod, a spring and a pressure block. The sampling rack is fixedly connected to the side wall of the research cabinet, and a material storage trough for placing plant samples is provided on the side of the sampling rack; a card slot for placing sample test tubes is provided at the lower end of the sampling rack; a through hole is provided at the upper end of the card slot; the punch rod is limitedly slidably connected to the upper end of the sampling rack, a pressure block is fixedly installed on the top of the material storage trough, the spring is sleeved on the outside of the punch rod, and the two ends of the spring are respectively fixedly connected to the sampling rack and the pressure block.

[0024] In order to better achieve the purpose of the present invention, the present invention also provides a device and method for studying pesticide metabolism in plants, comprising the following steps:

[0025] Step 1: Plant crops in each area of ​​the incubator, adjust the temperature in the research cabinet to the most suitable temperature for crop growth through the thermostat and temperature and humidity sensor, and add different trace elements to the corresponding root research box;

[0026] Step 2: The second control motor controls the winding and unwinding of the roller, so that the slider drives the fluorescent lamp to move along the arc frame to simulate the real sunrise and sunset effects in reality; the first control motor controls the turbine to rotate at the angle of the arc frame to simulate the sunrise and sunset directions in different months in reality, so that the sunlight effect in the research cabinet is close to that of the real farmland;

[0027] Step 3: The liquid storage barrel is driven to move vertically upward by the vertical servo motor, and the liquid in the liquid storage barrel flows into the temporary storage frame through the first connecting pipe according to the principle of the communicating vessel, until the liquid levels in the liquid storage barrel and the temporary storage frame are the same; the liquid in the temporary storage frame flows into the mixing cylinder through the second connecting pipe and is mixed, and the pushing cylinder uses the push rod to make the mixed pesticide liquid spray evenly on the crops in the cultivation box through the atomizing nozzle, and cooperates with the rotation of the rotating table to spray the crops in different areas of the cultivation box, and the pesticide concentration of the crops in each area is different; after spraying, the avoidance cylinder drives the mounting plate to move backward to avoid blocking the sunlight;

[0028] Step 4: During the growth of crops, regularly collect the stems, leaves and roots of crops in the incubator, put the samples into a rotating cleaning barrel and add clean liquid, then rotate the cleaning barrel to spin dry the samples, clean the pesticide residues on the surface of the samples, and push the samples out by controlling the bottom plate to move up through the servo push cylinder, so that the operator can take them out easily; put the clean samples into the material trough, and insert the sample test tube into the card slot, press the pressing block to make the punch rod punch the sample, and the punched sample is pushed into the sample test tube through the through hole, and the pesticide content in the sample is detected. By comparing the pesticide residues in the samples at different periods after the application of the pesticide, the plant's metabolic capacity for pesticides can be judged.

[0029] Compared with the prior art, the present invention has the following beneficial effects: 1. A "cross"-shaped partition is arranged in the cultivation box, the root system research box and the shielding cover, so that the inner cavity of the cultivation box, the root system research box and the shielding cover is divided into four areas of equal volume. By setting different parameters in different areas, the crops planted in each area can serve as control groups for each other;

[0030] Each area of ​​the incubator is equipped with soil for growing crops, and a wire mesh is installed at the bottom of each area of ​​the incubator. After the crops germinate, their roots will pass through the pores of the wire mesh and extend into the root research box at the bottom of the incubator. The operator can place different substances in the root research box for direct absorption by the crop roots to determine the effects of different experimental substances on pesticide metabolism.

[0031] 2. The sunlight track simulation component is used to control the movement of the fluorescent lamp along an arc track to simulate the sunrise and sunset of the sun. The azimuth simulation component is used to control the fluorescent lamp to rotate around the incubator to simulate the change of the sunrise position of the sun in different months. The shelf adopts electrically controlled dimming glass to simulate sunny and cloudy days by changing the transparency of the shelf. The azimuth simulation component, sunlight track simulation component, fluorescent lamp and shelf cooperate to achieve the simulation effect of real sunlight, reduce the influence of sunlight on the effect of pesticide residues in the experimental environment, and thus increase the accuracy of experimental research.

[0032] 3. The concentration control component is provided with two groups to control the amount of pesticide and water respectively, and the concentration of pesticide is adjusted by changing the ratio of pesticide to water; the liquid spraying component is used to spray the prepared liquid into a single area of ​​the incubator, and the incubator is rotated by rotating the supporting component to realize spraying different concentrations of pesticide in different areas of the incubator, so as to study the effect of pesticide concentration on the ability of plants to metabolize pesticides;

[0033] 4. The clean sample processed by the cleaning component is quickly sampled into the sample tube through the sampling component. By detecting the pesticide content in the sample and comparing the pesticide residues in the samples at different periods after the application of the pesticide, the plant's metabolic capacity for pesticides can be accurately determined. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0035] Figure 1 A three-dimensional diagram of a device for studying pesticide metabolism in plants according to the present invention;

[0036] Figure 2 It is a front view of a device for studying pesticide metabolism in plants according to the present invention;

[0037] Figure 3 It is a left view of a device for studying pesticide metabolism in plants according to the present invention;

[0038] Figure 4 is a three-dimensional diagram of the incubator of the present invention;

[0039] Figure 5 is a three-dimensional diagram of the sunlight simulation mechanism of the present invention;

[0040] Figure 6 The three-dimensional pesticide and moisture control mechanism of the present invention Figure 1 ;

[0041] Figure 7 The three-dimensional pesticide and moisture control mechanism of the present invention Figure 2 ;

[0042] Figure 8 The three-dimensional sample collection mechanism of the present invention Figure 1 ;

[0043] Fig. 9 The three-dimensional sample collection mechanism of the present invention Figure 2 .

[0044] The numbers in the figure represent:

[0045] 10. Research cabinet; 11. Cultivation box; 12. Root system research box; 13. Shielding cover; 14. Temperature and humidity sensor; 15. Temperature controller; 16. Shelf; 2. Rotating support assembly; 21. Rotating table; 22. Support; 23. Rotating motor; 24. Active guide wheel; 25. Driven guide wheel; 26. Accommodating slot; 27. Fixed plate; 28. Rotating plate; 3. Daylight simulation mechanism; 31. Azimuth simulation assembly; 311. First control motor; 312. Worm; 313. Turbine; 314. Limiting guide wheel; 315. Universal ball; 32. Daylight trajectory simulation assembly; 321. Arc frame; 322. Slider; 323. Second control motor; 324. Roller; 325. Traction rope; 33. Fluorescent lamp; 4. Pesticide and moisture control mechanism; 41. Concentration control assembly; 411. Vertical servo motor; 412, screw rod; 413, slide rod; 414, mounting frame; 415, vertical moving plate; 416, liquid storage barrel; 417, temporary storage frame; 418, first connecting pipe; 42, liquid spraying assembly; 421, avoidance cylinder; 422, mounting plate; 423, mixing cylinder; 424, atomizing nozzle; 425, push rod; 426, push cylinder; 427, second connecting pipe; 5, sample Collecting mechanism; 51, cleaning component; 511, rotating cleaning barrel; 512, bracket; 513, bottom plate; 514, connecting rod; 515, servo push cylinder; 516, gear; 517, gear ring; 518, cleaning motor; 52, sampling component; 521, sampling rack; 522, material trough; 523, card slot; 524, through hole; 525, punch rod; 526, spring; 527, pressure block. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] The terms “left”, “right”, “front”, “back”, “up” and “down” mentioned in the following description are oriented in the viewing direction of the front view.

[0048] Embodiment 1: In some embodiments, please refer to the drawings of the specification Figure 1-Figure 4 , a device for studying pesticide metabolism in plants, comprising a research cabinet 10;

[0049] The research cabinet 10 is provided with a rotating support assembly 2 at the bottom, and a cultivation box 11 is detachably connected to the movable end of the rotating support assembly 2. A root system research box 12 is placed at the lower side of the cultivation box 11, and a shielding cover 13 is connected to the upper side of the cultivation box 11 through a card block and a card slot structure; the shielding cover 13 can be made of acrylic material or glass material with excellent light transmission performance;

[0050] A "cross"-shaped partition is provided in the cultivation box 11, the root system research box 12 and the shielding cover 13, so that the inner cavity of the cultivation box 11, the root system research box 12 and the shielding cover 13 is divided into four areas of equal volume. By setting different parameters in different areas, the crops planted in each area can serve as control groups for each other;

[0051] Soil for growing crops is provided in each area of ​​the incubation box 11, and a wire mesh is provided at the bottom of each area of ​​the incubation box 11. After the crops germinate, their roots will pass through the pores of the wire mesh and extend into the root system research box 12 at the bottom of the incubation box 11. The operator can place different substances in the root system research box 12 for direct absorption by the crop roots to determine the effects of different experimental substances on pesticide metabolism.

[0052] A thermostat 15 is fixedly installed on the inner wall of the research cabinet 10, and a temperature and humidity sensor 14 is provided in each area of ​​the shielding cover 13. The temperature in the research cabinet 10 is adjusted to the temperature most suitable for crop growth through the cooperation of the thermostat 15 and the temperature and humidity sensor 14, thereby increasing the growth rate of the crops;

[0053] A shelf 16 is fixedly installed at the upper end of the inner cavity of the research cabinet 10, and a daylight simulation mechanism 3 is arranged on the shelf 16. The daylight simulation mechanism 3 includes an orientation simulation component 31, a daylight track simulation component 32 and a fluorescent lamp 33. The orientation simulation component 31 is arranged at the upper end of the shelf 16, the daylight track simulation component 32 is arranged at the moving end of the orientation simulation component 31, and the fluorescent lamp 33 is arranged at the moving end of the daylight track simulation component 32; the daylight track simulation component 32 is used to control the fluorescent lamp 33 to move along an arc track to simulate the sunrise and sunset of the sun, and the orientation simulation component 31 is used to control the fluorescent lamp 33 to rotate around the incubator 11 to simulate the change of the sunrise position of the sun in different months; the shelf 16 adopts an electrically controlled dimming glass, and simulates sunny and cloudy days by changing the transparency of the shelf 16; the orientation simulation component 31, the daylight track simulation component 32, the fluorescent lamp 33 and the shelf 16 cooperate to achieve the simulation effect of real daylight, reduce the influence of daylight on the effect of pesticide residues in the experimental environment, and thus increase the accuracy of experimental research;

[0054] The inner wall of the research cabinet 10 is provided with a pesticide and moisture control mechanism 4, which includes a concentration control component 41 and a liquid spray component 42, which are respectively provided on the outer wall and the inner wall of the research cabinet 10; the concentration control component 41 is provided with two groups for controlling the amount of pesticide and the amount of water, respectively, and the concentration of the pesticide is adjusted by changing the ratio of the pesticide to water; the liquid spray component 42 is used to spray the prepared liquid into a single area of ​​the cultivation box 11, and the cultivation box 11 is rotated by the rotating support component 2 to realize spraying different concentrations of pesticides in different areas of the cultivation box 11, so as to study the effect of the pesticide concentration on the ability of the plant to metabolize the pesticide, and the proportion of the pesticide can also be controlled to be zero to realize watering of the crops;

[0055] The left outer wall of the research cabinet 10 is provided with a sample collection mechanism 5 for removing the crops on the surface of the pesticides and then performing sampling operations to study the pesticide situation in the plants.

[0056] The orientation simulation component 31 includes a first control motor 311, a worm 312, a turbine 313, a limit guide wheel 314 and a universal ball 315. The first control motor 311 is fixedly mounted on the upper end of the layer plate 16, and the output end of the first control motor 311 is fixedly mounted with the worm 312; the outer side of the turbine 313 is meshedly connected with the worm 312; a plurality of limit guide wheels 314 are rotatably mounted on the upper end of the layer plate 16 and are rollingly connected with the inner side of the turbine 313; a plurality of universal ball 315 rollingly connected with the lower end of the turbine 313 are also arranged on the upper end of the layer plate 16;

[0057] The sunlight track simulation component 32 includes an arc frame 321, a slider 322 and a traction component. The arc frame 321 is fixedly installed on the inner side of the turbine 313, and the slider 322 is connected to the arc frame 321 in a limited sliding manner. The traction components for controlling the movement of the slider 322 are symmetrically arranged on both sides of the arc frame 321.

[0058] The traction assembly includes a second control motor 323, a roller 324 and a traction rope 325. The second control motor 323 is fixedly connected to the turbine 313. The output end of the second control motor 323 is fixedly installed with a roller 324 for winding the traction rope 325. The two ends of the traction rope 325 are respectively fixedly connected to the end of the slider 322 and the roller 324.

[0059] A plurality of fluorescent lamps 33 are fixedly mounted on the lower end of the slider 322;

[0060] In the present invention, the winding and unwinding of the rollers 324 on both sides are respectively controlled by the second control motors 323 on both sides, so that the slider 322 can be controlled to drive the fluorescent lamp 33 to move along the arc frame 321 to simulate the real sunrise and sunset effects; the first control motor 311 drives the worm 312 to rotate, so that the turbine 313 can be controlled to rotate under the limiting support of the limiting guide wheel 314 and the universal ball 315, and the angle of the arc frame 321 can be changed to simulate the sunrise and sunset directions in different months, so that the sunlight effect in the research cabinet 10 is close to that of the real farmland, reducing the influence of different sunlight effects on the experimental results.

[0061] The rotating support assembly 2 includes a rotating table 21, a support 22, a rotating motor 23, a driving guide wheel 24, a driven guide wheel 25, a receiving groove 26 and a limiting assembly. The support 22 is fixedly mounted on the inner bottom of the research cabinet 10, and a ball is arranged on the upper end of the support 22 to support the rotatable rotating table 21; the rotating motor 23 is fixedly mounted on the inner bottom of the research cabinet 10, and a driving guide wheel 24 is fixedly mounted on the output end of the rotating motor 23; a plurality of driven guide wheels 25 are rotatably mounted on the inner bottom of the research cabinet 10; the driving guide wheel 24 and the driven guide wheel 25 are both rollingly connected to the outer edge of the rotating table 21; a receiving groove 26 is provided on the rotating table 21, and the inner wall of the receiving groove 26 is limitedly slidably connected to the side wall of the incubator 11; and limiting assemblies for locking the incubator 11 in the receiving groove 26 are also arranged on both sides of the opening of the receiving groove 26 on the rotating table 21;

[0062] The limiting assembly includes a fixed plate 27 and a rotating plate 28, the fixed plate 27 is fixedly connected to the rotating table 21, and the upper end of the fixed plate 27 is rotatably connected to the end of the rotating plate 28;

[0063] In the present invention, the rotating motor 23 controls the rotation of the active guide wheel 24 to drive the rotating table 21 to rotate on the top of the rotating table 21 under the limiting action of the driven guide wheel 25; the cultivation box 11 can be pulled out in the receiving groove 26, which is convenient for the operator to observe the root growth status of the crop and directly sample the root system of the crop.

[0064] Embodiment 2: In some embodiments, Figure 5-Figure 9As shown, as a preferred embodiment of the present invention, the concentration control component 41 includes a vertical servo motor 411, a screw rod 412, a slide rod 413, a mounting frame 414, a vertical plate 415, a liquid storage barrel 416, a temporary storage frame 417 and a first connecting pipe 418, the mounting frame 414 is fixedly connected to the side wall of the research cabinet 10, the screw rod 412 is rotatably connected to the mounting frame 414 through a bearing, and the slide rod 413 is fixedly connected to the mounting frame 414; the vertical servo motor 411 is fixedly connected to the mounting frame 414, and the output end of the vertical servo motor 411 is fixedly connected to the screw rod 412 The vertical moving plate 415 is threadedly connected to the screw rod 412 through a threaded sleeve, and the vertical moving plate 415 is slidably connected to the sliding rod 413; the liquid storage barrel 416 is fixedly connected to the vertical moving plate 415, and the temporary storage frame 417 is fixedly connected to the side wall of the research cabinet 10. The lower end of the temporary storage frame 417 is connected to the lower end of the liquid storage barrel 416 through a first connecting pipe 418, so that the liquid storage barrel 416 and the temporary storage frame 417 form a communicating vessel structure, and the liquid levels in the liquid storage barrel 416 and the temporary storage frame 417 are the same; the liquid storage barrels 416 of the two concentration control components 41 are used to store pesticides and water respectively;

[0065] The liquid spraying assembly 42 includes an avoidance cylinder 421, a mounting plate 422, a liquid mixing cylinder 423, an atomizing nozzle 424, a push rod 425, a pushing cylinder 426 and a second connecting pipe 427. The avoidance cylinder 421 is fixedly connected to the outer wall of the research cabinet 10, and the output end of the avoidance cylinder 421 passes through the side wall of the research cabinet 10 and is fixedly connected to the mounting plate 422; the liquid mixing cylinder 423 and the pushing cylinder 426 are fixedly connected to the mounting plate 422, and the lower end of the liquid mixing cylinder 423 is provided with an atomizing nozzle 424 facing the incubator 11; the temporary storage frames 417 of the two concentration control assemblies 41 are respectively connected to the liquid mixing cylinder 423 through the second connecting pipe 427, and the water in the temporary storage frame 417 that is higher than the interface of the second connecting pipe 427 will flow into the liquid mixing cylinder 423 along the second connecting pipe 427; the output end of the pushing cylinder 426 is fixedly installed with a push rod 425 plugged into the liquid mixing cylinder 423;

[0066] In the present invention, the liquid storage barrel 416 is arranged outside the research cabinet 10, so that the operator can observe the liquid volume in the liquid storage barrel 416 and the liquid adding operation to the liquid storage barrel 416; in the initial state, the temporary storage frame 417 is at the same height as the liquid level in the liquid storage barrel 416, and the vertical servo motor 411 drives the screw rod 412 to rotate to control the vertical plate 415 to drive the liquid storage barrel 416 to move vertically upward under the limiting action of the slide bar 413. Under the principle of the communicating vessel, the liquid in the liquid storage barrel 416 flows into the temporary storage frame 417 through the first communicating pipe 418 until the liquid storage barrel 416 and the temporary storage frame are connected. The liquid level in 417 is the same, and the amount of liquid injected is equal to the cross-sectional size of the liquid storage barrel 416 multiplied by the lifting height of the liquid storage barrel 416, so as to accurately control the amount of pesticide and water: the liquid in the temporary storage frame 417 flows into the mixing barrel 423 through the second connecting pipe 427 and then mixes, and the push cylinder 426 drives the push rod 425 to insert into the mixing barrel 423 so that the mixed pesticide liquid is sprayed evenly to the crops in the cultivation box 11 through the atomizing nozzle 424, reducing the labor intensity, and cooperating with the rotation of the rotating table 21 to spray the crops in different areas of the cultivation box 11, which is effective and convenient for the plants. Research on the metabolism of pesticides in the plant; after spraying, the avoidance cylinder 421 drives the mounting plate 422 to move backward to avoid blocking the sunlight.

[0067] The sample collection mechanism 5 includes a cleaning component 51 and a sampling component 52, both of which are installed on the left side wall of the research cabinet 10. The cleaning component 51 is used to clean the pesticide on the surface of the crop, and the sampling component 52 is used to sample the cleaned crop;

[0068] The cleaning assembly 51 includes a rotating cleaning barrel 511, a bracket 512, a slot 523, a connecting rod 514, a servo push cylinder 515 and a rotating drive assembly. The bracket 512 is fixedly connected to the side wall of the research cabinet 10, and the rotating cleaning barrel 511 is rotatably connected to the bracket 512; a hollow cavity is provided between the inner layer and the outer layer of the rotating cleaning barrel 511, and a plurality of water leakage holes are evenly provided on the inner layer of the rotating cleaning barrel 511; the servo push cylinder 515 is fixedly connected to the side wall of the research cabinet 10, and the output end of the servo push cylinder 515 is rotatably connected to the lower end of the connecting rod 514 through a bearing, and the upper end of the connecting rod 514 is connected to the bottom of the rotating cleaning barrel 511 through a key, so that the connecting rod 514 can move vertically when rotating synchronously with the rotating cleaning barrel 511; the upper end of the connecting rod 514 extends into the inner layer of the rotating cleaning barrel 511 and is fixedly connected to the bottom plate 513; the side wall of the rotating cleaning barrel 511 is provided with a rotating drive assembly;

[0069] The rotary drive assembly includes a gear 516, a gear ring 517 and a cleaning motor 518. The cleaning motor 518 is fixedly connected to the side wall of the research cabinet 10. The output end of the cleaning motor 518 is fixedly installed with a gear 516. The lower end of the rotating cleaning barrel 511 is fixedly installed with a gear ring 517 meshing with the gear 516. The cleaning motor 518 can drive the rotating cleaning barrel 511 to rotate through the transmission effect of the gear 516 and the gear ring 517:

[0070] The sampling assembly 52 includes a sampling frame 521, a material placement groove 522, a card slot 523, a through hole 524, a punch rod 525, a spring 526 and a pressure block 527. The sampling frame 521 is fixedly connected to the side wall of the research cabinet 10. The side of the sampling frame 521 is provided with a material placement groove 522 for placing plant samples; the lower end of the sampling frame 521 is provided with a card slot 523 for placing sample test tubes; the upper end of the card slot 523 is provided with a through hole 524; the punch rod 525 is limitedly slidably connected to the upper end of the sampling frame 521, and a pressure block 527 is fixedly installed on the top of the material placement groove 522. The spring 526 is sleeved on the outside of the punch rod 525, and the two ends of the spring 526 are respectively fixedly connected to the sampling frame 521 and the pressure block 527;

[0071] In the present invention, after sampling the plant, the sample is placed in a rotating cleaning barrel 511 and clean liquid is added. The rotating cleaning barrel 511 is then rotated to spin dry the sample to clean the surface of the sample. The servo push cylinder 515 can be used to control the bottom plate 513 to move upward to push out the sample, so that the operator can take it easily. The clean sample is placed in the material trough 522, and the sample test tube is inserted into the card slot 523. The pressing block 527 is pressed to make the punch rod 525 punch the sample, and the punched sample is pushed into the sample test tube through the through hole 524. By detecting the pesticide content in the sample, an accurate study of the pesticide metabolism in the plant is achieved.

[0072] Embodiment 3: In some embodiments, Figure 1-Figure 9 As shown, as a preferred embodiment of the present invention, a method for studying pesticide metabolism in plants comprises the following steps:

[0073] Step 1: Plant crops in each area of ​​the cultivation box 11, adjust the temperature in the research cabinet 10 to the temperature most suitable for crop growth through the thermostat 15 and the temperature and humidity sensor 14, and add different trace elements to the corresponding root research box 12;

[0074] Step 2: The second control motor 323 controls the winding and unwinding of the roller 324, so that the slider 322 drives the fluorescent lamp 33 to move along the arc frame 321, simulating the real sunrise and sunset effects in reality; the first control motor 311 controls the turbine 313 to rotate at the angle of the arc frame 321, simulating the sunrise and sunset directions in different months in reality, so that the sunlight effect in the research cabinet 10 is close to that of a real farmland;

[0075] Step 3: The liquid storage barrel 416 is driven to move vertically upward by the vertical servo motor 411, and the liquid in the liquid storage barrel 416 flows into the temporary storage frame 417 through the first connecting pipe 418 according to the principle of the communicating vessel, until the liquid levels in the liquid storage barrel 416 and the temporary storage frame 417 are the same; the liquid in the temporary storage frame 417 flows into the mixing cylinder 423 through the second connecting pipe 427 and is mixed, and the pushing cylinder 426 uses the push rod 425 to make the mixed pesticide liquid spray evenly on the crops in the cultivation box 11 through the atomizing nozzle 424, and cooperates with the rotation of the rotating table 21 to spray the crops in different areas of the cultivation box 11, and the pesticide concentration of the crops in each area is different; after spraying, the avoidance cylinder 421 drives the mounting plate 422 to move backward to avoid blocking the sunlight;

[0076] Step 4: During the growth of crops, the stems, leaves and roots of the crops in the cultivation box 11 are collected regularly, and the samples are placed in the rotating cleaning barrel 511 and clean liquid is added. The rotating cleaning barrel 511 is then rotated to spin dry the samples, and the pesticide residues on the surface of the samples are cleaned. The servo push cylinder 515 can be used to control the bottom plate 513 to move upward to push out the samples for the convenience of the operator to take them. The clean samples are placed in the material trough 522, and the sample test tube is inserted into the card slot 523. The pressing block 527 is pressed to make the punch rod 525 punch the sample, and the punched sample is pushed into the sample test tube through the through hole 524, and the pesticide content in the sample is detected. By comparing the pesticide residues in the samples at different periods after the application of the pesticide, the plant's metabolic capacity for pesticides is judged.

[0077] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A device for studying pesticide metabolism in plants, comprising a research cabinet (10), characterized in that: The research cabinet (10) is provided with a rotating support assembly (2) at the bottom thereof, the movable end of the rotating support assembly (2) is detachably connected to a cultivation box (11), a root system research box (12) is placed at the bottom of the cultivation box (11), and a shielding cover (13) is connected to the top of the cultivation box (11) via a clamping block and a clamping slot structure; A "cross"-shaped partition is arranged inside the cultivation box (11), the root system research box (12) and the shielding cover (13), so that the inner cavity of the cultivation box (11), the root system research box (12) and the shielding cover (13) is divided into four areas of equal volume; a wire mesh is arranged at the bottom of each area of ​​the cultivation box (11); A temperature controller (15) is fixedly installed on the inner wall of the research cabinet (10), and a temperature and humidity sensor (14) is provided in each area of ​​the shielding cover (13): A layer plate (16) is fixedly mounted on the upper end of the inner cavity of the research cabinet (10), and a daylight simulation mechanism (3) is arranged on the layer plate (16). The daylight simulation mechanism (3) comprises an orientation simulation component (31), a daylight track simulation component (32) and a fluorescent lamp (33). The orientation simulation component (31) is arranged on the upper end of the layer plate (16), the daylight track simulation component (32) is arranged at the moving end of the orientation simulation component (31), and the fluorescent lamp (33) is arranged at the moving end of the daylight track simulation component (32). The daylight track simulation component (32) is used to control the fluorescent lamp (33) to move along an arc track to simulate the sunrise and sunset of the sun, and the orientation simulation component (31) is used to control the fluorescent lamp (33) to rotate around the incubator (11) to simulate the change in the sunrise position of the sun in different months. The layer plate (16) adopts electrically controlled dimming glass. The inner wall of the research cabinet (10) is provided with a pesticide and water control mechanism (4), the pesticide and water control mechanism (4) comprises a concentration control component (41) and a liquid spray component (42), the concentration control component (41) and the liquid spray component (42) are respectively provided on the outer wall and the inner wall of the research cabinet (10); the concentration control component (41) is provided with two groups for controlling the amount of pesticide and the amount of water respectively, and the concentration of the pesticide is adjusted by changing the ratio of the pesticide to the water; the liquid spray component (42) is used to spray the prepared liquid into a single area of ​​the incubator (11); The left outer wall of the research cabinet (10) is provided with a sample collection mechanism (5) for removing the crops on the surface of the pesticide and then performing sampling operations.

2. The device for studying pesticide metabolism in plants according to claim 1, characterized in that: The orientation simulation component (31) comprises a first control motor (311), a worm (312), a turbine (313), a limit guide wheel (314) and a universal ball (315); the first control motor (311) is fixedly mounted on the upper end of the layer plate (16); the output end of the first control motor (311) is fixedly mounted with the worm (312); the outer side of the turbine (313) is meshingly connected with the worm (312); a plurality of limit guide wheels (314) are rotatably mounted on the upper end of the layer plate (16) and are rollingly connected with the inner side of the turbine (313); and a plurality of universal ball (315) rollingly connected with the lower end of the turbine (313) are also arranged on the upper end of the layer plate (16).

3. The device for studying pesticide metabolism in plants according to claim 2, characterized in that: The sunlight track simulation component (32) comprises an arc frame (321), a slider (322) and a traction component, wherein the arc frame (321) is fixedly mounted on the inner side of the turbine (313), and the slider (322) is connected to the arc frame (321) in a limited sliding manner; and the traction components for controlling the movement of the slider (322) are symmetrically arranged on both sides of the arc frame (321); The traction assembly comprises a second control motor (323), a roller (324) and a traction rope (325); the second control motor (323) is fixedly connected to the turbine (313); a roller (324) for winding the traction rope (325) is fixedly installed at the output end of the second control motor (323); two ends of the traction rope (325) are respectively fixedly connected to the end of the slider (322) and the roller (324); A plurality of fluorescent lamps (33) are fixedly mounted on the lower end of the slider (322).

4. The device for studying pesticide metabolism in plants according to claim 3, characterized in that: The rotating support assembly (2) comprises a rotating table (21), a support (22), a rotating motor (23), a driving guide wheel (24), a driven guide wheel (25), a receiving groove (26) and a limiting assembly. The support (22) is fixedly mounted on the inner bottom of the research cabinet (10). A ball bearing is arranged on the upper end of the support (22) to support the rotatable rotating table (21). The rotating motor (23) is fixedly mounted on the inner bottom of the research cabinet (10). The output end of the rotating motor (23) is fixedly mounted with a driving guide wheel. Wheel (24): a plurality of driven guide wheels (25) are rotatably mounted on the inner bottom of the research cabinet (10); the driving guide wheel (24) and the driven guide wheel (25) are both rollingly connected to the outer edge of the rotating platform (21); a receiving groove (26) is provided on the rotating platform (21), and the inner wall of the receiving groove (26) is limitedly slidably connected to the side wall of the incubator (11); and limiting components for locking the incubator (11) in the receiving groove (26) are also provided on both sides of the opening of the receiving groove (26) on the rotating platform (21).

5. The device for studying pesticide metabolism in plants according to claim 4, characterized in that: The concentration control component (41) comprises a vertical movement servo motor (411), a screw rod (412), a slide rod (413), a mounting frame (414), a vertical movement plate (415), a liquid storage barrel (416), a temporary storage frame (417) and a first connecting pipe (418); the mounting frame (414) is fixedly connected to the side wall of the research cabinet (10); the screw rod (412) is rotatably connected to the mounting frame (414) through a bearing; the slide rod (413) is fixedly connected to the mounting frame (414); the vertical movement servo motor (411) is fixedly connected to the mounting frame (414); the output end of the vertical movement servo motor (411) is connected to the screw rod (416); 412) is fixedly connected; the vertical moving plate (415) is threadedly connected to the screw rod (412) through a threaded sleeve, and the vertical moving plate (415) is slidably connected to the sliding rod (413); the liquid storage barrel (416) is fixedly connected to the vertical moving plate (415), and the temporary storage frame (417) is fixedly connected to the side wall of the research cabinet (10), and the lower end of the temporary storage frame (417) is connected to the lower end of the liquid storage barrel (416) through a first connecting pipe (418), so that the liquid storage barrel (416) and the temporary storage frame (417) form a connecting vessel structure; the liquid storage barrels (416) of the two concentration control components (41) are used to store pesticides and water respectively.

6. The device for studying pesticide metabolism in plants according to claim 5, characterized in that: The liquid spraying assembly (42) comprises a bypass cylinder (421), a mounting plate (422), a liquid mixing cylinder (423), an atomizing nozzle (424), a push rod (425), a push cylinder (426) and a second connecting pipe (427); the bypass cylinder (421) is fixedly connected to the outer wall of the research cabinet (10); the output end of the bypass cylinder (421) passes through the side wall of the research cabinet (10) and is fixedly connected to the mounting plate (422); the liquid mixing cylinder (423) is fixedly connected to the mounting plate (422); 23) and a pushing cylinder (426) are fixedly connected to the mounting plate (422); the lower end of the mixing cylinder (423) is provided with an atomizing nozzle (424) facing the incubator (11); the temporary storage frames (417) of the two concentration control components (41) are respectively connected to the mixing cylinder (423) through second connecting pipes (427); and the output end of the pushing cylinder (426) is fixedly provided with a push rod (425) plugged into the mixing cylinder (423).

7. The device for studying pesticide metabolism in plants according to claim 6, characterized in that: The sample collection mechanism (5) comprises a cleaning component (51) and a sampling component (52), both of which are mounted on the left side wall of the research cabinet (10), the cleaning component (51) is used to clean the pesticide on the surface of the crop, and the sampling component (52) is used to sample the cleaned crop.

8. The device for studying pesticide metabolism in plants according to claim 7, characterized in that: The cleaning assembly (51) comprises a rotating cleaning barrel (511), a bracket (512), a slot (523), a connecting rod (514), a servo push cylinder (515) and a rotating drive assembly. The bracket (512) is fixedly connected to the side wall of the research cabinet (10), and the rotating cleaning barrel (511) is rotatably connected to the bracket (512). A hollow cavity is provided between the inner layer and the outer layer of the rotating cleaning barrel (511), and a plurality of water leakage holes are evenly provided on the inner layer of the rotating cleaning barrel (511). The servo push cylinder (515) is connected to the rotating driving assembly. The research cabinet (10) is fixedly connected to the side wall, the output end of the servo push cylinder (515) is rotatably connected to the lower end of the connecting rod (514) through a bearing, and the upper end of the connecting rod (514) is connected to the bottom of the rotating cleaning barrel (511) through a key, so that the connecting rod (514) can move vertically when rotating synchronously with the rotating cleaning barrel (511); the upper end of the connecting rod (514) extends into the inner layer of the rotating cleaning barrel (511) and is fixedly connected to the bottom plate (513); the side wall of the rotating cleaning barrel (511) is provided with a rotating drive assembly.

9. The device for studying pesticide metabolism in plants according to claim 8, characterized in that: The sampling assembly (52) comprises a sampling frame (521), a material placement groove (522), a card slot (523), a through hole (524), a punch rod (525), a spring (526) and a pressure block (527); the sampling frame (521) is fixedly connected to the side wall of the research cabinet (10); a material placement groove (522) for placing plant samples is provided on the side of the sampling frame (521); a useful A card slot (523) is provided for placing a sample test tube; a through hole (524) is provided at the upper end of the card slot (523); a punch rod (525) is limitedly slidably connected to the upper end of the sampling frame (521); a pressing block (527) is fixedly installed on the top of the material placement groove (522); a spring (526) is sleeved on the outside of the punch rod (525), and two ends of the spring (526) are fixedly connected to the sampling frame (521) and the pressing block (527) respectively.

10. A method for studying pesticide metabolism in plants using the pesticide metabolism in plants research device according to claim 9, characterized in that: The following steps are involved: Step 1: Planting crops in each area of ​​the cultivation box (11), adjusting the temperature in the research cabinet (10) to the temperature most suitable for crop growth through the cooperation of the temperature controller (15) and the temperature and humidity sensor (14), and adding different trace elements into the corresponding root system research box (12); Step 2: The second control motor (323) controls the winding and unwinding of the roller (324), so that the slider (322) drives the fluorescent lamp (33) to move along the arc frame (321), simulating the real sunrise and sunset effects in reality; the first control motor (311) controls the turbine (313) to rotate at the angle of the arc frame (321), simulating the sunrise and sunset directions in different months in reality, so that the sunlight effect in the research cabinet (10) is close to that of a real farmland; Step 3: The liquid storage barrel (416) is driven to move vertically upward by a vertical servo motor (411), and the liquid in the liquid storage barrel (416) flows into the temporary storage frame (417) through the first connecting pipe (418) under the principle of a communicating vessel, until the liquid levels in the liquid storage barrel (416) and the temporary storage frame (417) are the same; the liquid in the temporary storage frame (417) flows into the mixing cylinder (423) through the second connecting pipe (427) and is mixed, and the pushing cylinder (426) causes the mixed pesticide liquid to be evenly sprayed onto the crops in the cultivation box (11) through the atomizing nozzle (424) through the push rod (425), and the crops in different areas of the cultivation box (11) are sprayed with liquid in coordination with the rotation of the rotating platform (21), and the pesticide concentration of the crops in each area is different; after spraying, the avoiding cylinder (421) drives the mounting plate (422) to move backward to avoid blocking sunlight; Step 4: During the crop growth process, the stems, leaves and roots of the crops in the cultivation box (11) are collected regularly, and the samples are placed in the rotating cleaning barrel (511) and clean liquid is added. The rotating cleaning barrel (511) is then rotated to dry the samples, and the pesticide residues on the surface of the samples are cleaned. The servo push cylinder (515) can be used to control the bottom plate (513) to move upward to push out the samples, so that the operator can take them easily. A clean sample is placed in the material placement slot (522), and the sample test tube is inserted into the card slot (523). The pressing block (527) is pressed to make the punch rod (525) punch a hole in the sample, and the punched sample is pushed into the sample test tube through the through hole (524). The pesticide content in the sample is detected, and the pesticide residue samples in the sample at different periods after the application of the pesticide are compared to determine the plant's ability to metabolize the pesticide.

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