Skin care composition as well as preparation method and application thereof

Abstract

The invention discloses a skin care composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The skin care composition disclosed by the invention consists of hyaluronic acid and / or salts thereof, ectoine and / or derivatives thereof, vitamins, amino acids, a buffer solute and water, or consists of carbohydrates, vitamins, amino acids, inorganic salts, coenzymes and water. The skin care composition provided by the invention is mild and non-irritant, is safe to a human body, has no side effect, has whitening, anti-oxidation and anti-inflammatory effects on skin after being used, and is simple to prepare.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a skin care composition and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Skin whitening has always been a hot topic in the beauty industry. With the pursuit of beauty and health by modern people, skin whitening has attracted more and more attention from the general public. However, due to various factors, such as ultraviolet radiation, changes in hormone levels, genetic factors, etc., people's skin often loses its original whitening feeling due to pigmentation, dullness and other problems, affecting appearance and self-confidence. At present, there are many types of whitening products on the market. Facial products include lotions, essences, masks, creams and other types. Body products include body lotions, body creams, hand masks, foot masks, scrubs and other endless products. The efficacy and ingredients of these products are also different. Some of them achieve whitening effects by blocking the synthesis of melanin, inhibiting the transfer and storage of melanin, and promoting melanin metabolism; while others achieve whitening effects by removing spots and keratin on the surface of the skin, improving skin brightness and transparency, and making the skin look smoother and brighter. However, not all whitening products on the market have high quality and good effects. Some products may illegally add harmful ingredients, such as hydroquinone, which may irritate the skin, damage the skin barrier, and cause skin sensitivity and other adverse reactions.

[0004] Oxidative stress is a recognized biological aging mechanism. Its core content is that the reactive oxygen generated by the body's metabolism attacks macromolecules (including DNA, proteins, lipids, etc.) in cells, causing oxidative damage. The continuous accumulation of oxidative damage substances in the body leads to the aging of the organism. Under normal circumstances, the body's antioxidant capacity maintains a dynamic balance with the continuously generated oxygen free radicals. As long as the excessive oxygen free radicals are removed in time, tissue oxidative damage can be avoided. However, if the production of oxygen free radicals increases abnormally and exceeds the removal capacity, or the body's antioxidant capacity decreases, resulting in the breakdown of the dynamic balance, it will cause oxidative damage to the tissue, induce disease, and accelerate aging.

[0005] Inflammation can be understood as "inflammation", which is a protective mechanism designed to control harmful factors and remove damaged tissues. The body's self-defense reaction to stimulation caused by external reactions is called inflammatory reaction. During the reaction, inflammatory factors in the human body are released, which will stimulate the human body and form swelling in the human body. Common inflammatory reactions of the skin are redness, swelling, heat, and pain. Redness and heat are caused by the expansion of capillaries and increased permeability in the inflamed area, which leads to an increase in local oxygenated hemoglobin, so it appears red; fever is caused by increased blood flow and enhanced metabolism in local tissues; swelling and pain are mainly caused by the stimulation of inflammatory substances, which causes local tissue cells to become congested and swollen, and the swollen tissue compresses the peripheral nerves, resulting in a feeling of pain. Inflammatory factors mainly refer to various cytokines involved in inflammatory reactions, which are mainly classified into TNF-α, IL-1β, IL-6, etc. Among them, IL-6 can induce B cell differentiation and antibody production, and induce T cell activation, proliferation and differentiation, participate in the body's immune response, and is a promoter of inflammatory response; TNF-α is the earliest and most important inflammatory mediator in the inflammatory response process, which can activate neutrophils and lymphocytes, increase the permeability of vascular endothelial cells, regulate the metabolic activity of other tissues and promote the synthesis and release of other cytokines.

[0006] Currently, there are varying quality skin care products on the market, and there is a lack of a safe and effective skin care product that combines whitening, anti-oxidation, and anti-inflammatory properties.

[0007] In view of the long period of whitening effect of skin care products, Chinese patent application CN110812320A discloses a whitening skin care composition, which includes the following components by mass fraction: 10-30 parts of rice amino acids; 20-40 parts of nonapeptide-1; 5-15 parts of bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate; 10-30 parts of yeast fermentation product. By synergizing rice amino acids, nonapeptide-1, bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate, and yeast fermentation product, the whitening skin care composition is added to the skin care products, which is beneficial to shorten the period of whitening effect of the skin care products, improve the whitening effect of the skin care products, and at the same time, it is beneficial to improve the anti-wrinkle function of the skin and delay skin aging.

[0008] Chinese patent application CN116173189A discloses a skin care composition, which is composed of the following components in parts by weight: 1-50 parts of blood orange powder, 10-1000 parts of collagen peptides, and 0.1-50 parts of vitamin C; the above-mentioned skin care composition, through the synergistic effect of blood orange powder, collagen peptides and vitamin C, can effectively increase skin moisture, reduce skin melanin, reduce skin fine lines, and has the effects of whitening, moisturizing and delaying aging.

[0009] Chinese patent application CN104189952A discloses an injection for correcting skin wrinkles, which contains: 3-6g / L high molecular weight polysaccharide; 50-200mg / L amino acid; 2-10mg / L water-soluble vitamin; 2-5g / L L-carnosine. The sample prepared according to this formula can supplement the skin with extracellular matrix, promote the synthesis of the skin's own collagen, solve the problem of skin wrinkles and sagging caused by aging, and is more suitable for intradermal injection to achieve anti-wrinkle and skin tightening effects. Summary of the invention

[0010] In order to solve the deficiencies of the prior art, the purpose of the present invention is to provide a skin care composition and a preparation method and application thereof. The skin care composition provided by the present invention has whitening, anti-inflammatory and antioxidant effects at the same time, and the composition is mild and non-irritating, safe to the human body and has no side effects.

[0011] In order to achieve the above object, the technical solution of the present invention is:

[0012] The first aspect of the present invention provides a skin care composition, which includes, by mass percentage, 0.01-5% hyaluronic acid and / or its salt, 0.01-6% ectoine and / or its derivatives, 0.00005-0.2% vitamins, 0.01-0.3% amino acids, 0.01-0.04% buffer solutes, and the balance is water.

[0013] In some embodiments of the present invention, the molecular weight of the hyaluronic acid and / or its salt is 5000-200W Da, preferably 50W-100W Da.

[0014] In some embodiments of the present invention, the hyaluronate is selected from at least one of sodium hyaluronate, zinc hyaluronate, potassium hyaluronate, magnesium hyaluronate and calcium hyaluronate, preferably sodium hyaluronate.

[0015] In some embodiments of the present invention, the ectoine derivative is one of hydroxyectoine, ectoine sodium salt and ectoine potassium salt,

[0016] In some embodiments of the present invention, the vitamin is selected from at least one of vitamin B and vitamin C, and the vitamin B is selected from at least one of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, niacinamide, and α-lipoic acid; preferably, the vitamin is a mixture of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, niacinamide and α-lipoic acid.

[0017] In some embodiments of the present invention, the amino acid is selected from at least one of glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine, and alanine.

[0018] In some embodiments of the present invention, the buffer solute is selected from at least one of 4-hydroxyethylpiperazineethane sulfonic acid and sodium 2-hydroxypyruvate; further preferably, in terms of mass percentage, the content of 4-hydroxyethylpiperazineethane sulfonic acid is 0.03%, and the content of sodium 2-hydroxypyruvate is 0.01%.

[0019] In some embodiments of the present invention, the skin care composition includes, by mass percentage: 0.05-3% hyaluronic acid and / or its salts, 0.05-5% ectoine and / or its derivatives, 0.0001-0.1% vitamins, 0.03-0.2% amino acids, 0.01-0.04% buffer solutes, and the balance is water.

[0020] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 0.05-3% sodium hyaluronate, 0.05-5% ectoine, 0.0001-0.1% vitamins, 0.03-0.2% amino acids, 0.04% buffer solutes, and the balance is water. The molecular weight of the sodium hyaluronate is 50W-100W Da.

[0021] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 2% sodium hyaluronate, 2% ectoine, 0.00035% vitamins, 0.043% amino acids, 0.04% buffer solutes, and the balance is water. The molecular weight of the sodium hyaluronate is 80W Da.

[0022] The second aspect of the present invention provides a method for preparing the skin care composition, comprising the following steps:

[0023] Dissolve the buffer solute in water to obtain solution A;

[0024] Adding ectoine and / or its derivatives, vitamins and amino acids to solution A to obtain solution B;

[0025] Hyaluronic acid and / or its salt is added to solution B to obtain a skin care composition.

[0026] In some embodiments of the present invention, hyaluronic acid and / or its salt is added to solution B and then sterilized to obtain a skin care composition; preferably, the sterilization treatment includes filtration sterilization, ethylene oxide sterilization, irradiation sterilization, moist heat sterilization, preferably moist heat sterilization.

[0027] The third aspect of the present invention provides a use of the skin care composition in preparing a product having whitening, anti-inflammatory and antioxidant effects.

[0028] In some embodiments of the present invention, the dosage form of the product is a solution, a gel, a spray, drops or a lotion, preferably a gel.

[0029] In some embodiments of the present invention, the product is a cosmetic, a medical device or a medicine.

[0030] In some embodiments of the present invention, the product is a medical device.

[0031] The fourth aspect of the present invention provides a skin care composition, which comprises, by mass percentage: 0.1-5% carbohydrate, 0.000001-0.0001% vitamin, 0.00001-0.1% amino acid, 0.01-0.1% inorganic salt, 0.000001-0.0005% coenzyme, and the balance is water.

[0032] In some embodiments of the present invention, the name of the skin care composition is "CTXX", wherein "XX" in "CTXX" can be any combination of Arabic numerals, Chinese characters and other alphabetic characters, preferably any combination of Arabic numerals, such as "CT10", "CT20", "CT30", "CT40", "CT50", "CT60", "CT70", "CT80", "CT90".

[0033] In some embodiments of the present invention, a buffer solute is also included.

[0034] Preferably, the buffer solute is at least one of 4-hydroxyethylpiperazineethanesulfonic acid and sodium 2-hydroxypyruvate. Further preferably, in terms of mass percentage, the content of 4-hydroxyethylpiperazineethanesulfonic acid is 0.03%, and the content of sodium 2-hydroxypyruvate is 0.01%.

[0035] In some embodiments of the present invention, the carbohydrate substance is selected from at least one of glucose, chitosan, hyaluronic acid and / or hyaluronate, and sodium carboxymethyl cellulose; preferably, the carbohydrate substance is hyaluronic acid and / or hyaluronate.

[0036] In some embodiments of the present invention, the hyaluronate is at least one of sodium hyaluronate, zinc hyaluronate, potassium hyaluronate, magnesium hyaluronate and calcium hyaluronate; preferably, the hyaluronate is sodium hyaluronate.

[0037] In some embodiments of the present invention, the molecular weight of the hyaluronic acid and / or hyaluronate is 5000-200 WDa, preferably 50W-100W Da.

[0038] In some embodiments of the present invention, the vitamin is selected from at least one of vitamin B, vitamin C, and adenine sulfate; wherein the vitamin B is selected from at least one of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, nicotinamide, and α-lipoic acid; preferably, the vitamin is a mixture of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B9, vitamin B12, adenine sulfate, biotin, nicotinamide, and α-lipoic acid.

[0039] In some embodiments of the present invention, the amino acid is selected from at least one of arginine, lysine, histidine, aspartic acid, glutamic acid, glycine, alanine, valine, leucine, isoleucine, phenylalanine, proline, tryptophan, serine, tyrosine, cysteine, methionine, asparagine, glutamine, and threonine.

[0040] In some embodiments of the present invention, the inorganic salt is selected from at least one of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid, sodium citrate, and sodium metasilicate; preferably, the inorganic salt is a mixture of disodium hydrogen phosphate, sodium chloride, and sodium metasilicate.

[0041] In some embodiments of the present invention, the coenzyme is α-lipoic acid.

[0042] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 0.05-3% carbohydrate, 0.00001-0.00005% vitamin, 0.0001-0.001% amino acid, 0.03-0.08% inorganic salt, 0.00001-0.00005% coenzyme, and the balance is water.

[0043] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 0.05-3% carbohydrate, 0.00001-0.00005% vitamin, 0.0001-0.001% amino acid, 0.03-0.08% inorganic salt, 0.00001-0.00005% coenzyme, and the balance is water; the carbohydrate is sodium hyaluronate and glucose; the vitamin is vitamin B5, vitamin B2, biotin, vitamin B 12. The invention relates to a pharmaceutical composition comprising folic acid, nicotinamide, vitamin B6, vitamin B1 and adenine sulfate; the amino acids comprise aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salt comprises disodium hydrogen phosphate, potassium chloride and sodium metasilicate; and the coenzyme is α-lipoic acid.

[0044] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 0.05% carbohydrate, 0.00001% vitamin, 0.0001% amino acid, 0.03% inorganic salt, 0.00001% coenzyme, and the balance is water; the carbohydrate is sodium hyaluronate and glucose; the vitamin is composed of vitamin B5, vitamin B2, biotin, vitamin B12, folic acid, niacinamide, vitamin B6, vitamin B1 and adenine sulfate; the amino acid is composed of aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salt is composed of disodium hydrogen phosphate, potassium chloride and sodium metasilicate; the coenzyme is α-lipoic acid.

[0045] In some embodiments of the present invention, the skin care composition comprises, by mass percentage: 3% carbohydrates, 0.00005% vitamins, 0.001% amino acids, 0.08% inorganic salts, 0.00005% coenzymes, and the balance is water; the carbohydrates are sodium hyaluronate and glucose; the vitamins are composed of vitamin B5, vitamin B2, biotin, vitamin B12, folic acid, niacinamide, vitamin B6, vitamin B1 and adenine sulfate; the amino acids are composed of aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salts are composed of disodium hydrogen phosphate, potassium chloride and sodium metasilicate; the coenzyme is α-lipoic acid.

[0046] A fifth aspect of the present invention provides a method for preparing the skin care composition, comprising the following steps:

[0047] Dissolve vitamins, amino acids, inorganic salts and coenzymes in water to obtain solution C;

[0048] A sugar substance is added to solution C to obtain a skin care composition.

[0049] In some embodiments of the present invention, after adding the sugar substance to solution C, sterilization treatment is performed to obtain a skin care composition; preferably, the sterilization treatment includes filtration sterilization, ethylene oxide sterilization, irradiation sterilization, moist heat sterilization, preferably moist heat sterilization.

[0050] In some embodiments of the present invention, the preparation method comprises dissolving the auxiliary material in water, and then adding vitamins, amino acids, inorganic salts and coenzymes to obtain solution C.

[0051] The sixth aspect of the present invention provides a use of the skin care composition in preparing a product having whitening, anti-inflammatory and antioxidant effects.

[0052] In some embodiments of the present invention, the dosage form of the product is a solution, a gel, a spray, drops or a lotion, preferably a gel.

[0053] In some embodiments of the present invention, the product is a cosmetic, a medical device or a medicine.

[0054] In some embodiments of the present invention, the product is a medical device.

[0055] The beneficial effects of the present invention are:

[0056] The present invention provides a skin care composition, which has whitening, anti-oxidation and anti-inflammatory effects through the combination of polysaccharides, vitamins, amino acids, coenzymes, inorganic salts and other substances. The composition is mild and non-irritating, safe to the human body and has no side effects. After use, it has whitening, anti-oxidation and anti-inflammatory effects on the skin. It has various functions and can be used for skin care of the head, face, neck, hands and other parts of the body.

[0057] The present invention provides a skin care composition, which has whitening, anti-oxidation and anti-inflammatory effects through the combination of vitamins, hyaluronic acid, amino acids, ectoine and other substances. The composition is mild and non-irritating, safe to the human body and has no side effects. After use, it has whitening, anti-oxidation and anti-inflammatory effects on the skin, has multiple functions, and can be used for the care of the head, face, neck, hands and other body skin. DETAILED DESCRIPTION

[0058] In order to enable those skilled in the art to more clearly understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below in conjunction with specific embodiments.

[0059] Example 1

[0060] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is as follows:

[0061] Dissolve the buffer solute (0.03% 4-hydroxyethylpiperazineethanesulfonic acid, 0.01% sodium 2-hydroxypyruvate) in water for injection and filter to obtain solution A;

[0062] Amino acids (glutamic acid 0.001%, glutamine 0.001%, histidine 0.0001%, isoleucine 0.0001%, leucine 0.0001%, lysine hydrochloride 0.001%, methionine 0.0001%, phenylalanine 0.0001%, serine 0.0001%, aspartic acid 0.001%, proline 0.001%, glycine 0.0003%, threonine 0.001%, valine 0.001%, arginine hydrochloride 0.001%, tyrosine 0.0001%, alanine 0.001%), vitamins (calcium pantothenate 0.00001%, vitamin B2 0.000002%, D-biotin 0.000008%, vitamin B12 0.00002%, folic acid 0.000002%, niacinamide 0.000002%, vitamin B6 0.000003%, vitamin B1 0.000002%, α-lipoic acid 0.000001%) and ectoine are added to solution A and dissolved to obtain solution B;

[0063] Sodium hyaluronate was added to solution B and dissolved to obtain a skin care composition.

[0064] Example 2

[0065] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0066] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0067] Amino acids (glutamic acid 0.003%, glutamine 0.003%, histidine 0.04%, isoleucine 0.0002%, leucine 0.0002%, lysine hydrochloride 0.003%, methionine 0.04%, phenylalanine 0.08%, serine 0.0002%, aspartic acid 0.003%, proline 0.002%, glycine 0.06%, threonine 0.002%, valine 0.002%, arginine hydrochloride 0.001%, tyrosine 0.0004%, alanine 0.06%).

[0068] Vitamins (calcium pantothenate 0.01%, vitamin B2 0.02%, D-biotin 0.07%, vitamin B12 0.06%, folic acid 0.01%, niacinamide 0.01%, vitamin B6 0.006%, vitamin B1 0.004%, alpha-lipoic acid 0.01%).

[0069] Example 3

[0070] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0071] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0072] Amino acids (glutamic acid 0.00003%, glutamine 0.00003%, histidine 0.0004%, isoleucine 0.000002%, leucine 0.000002%, lysine hydrochloride 0.00003%, methionine 0.0004%, phenylalanine 0.0008%, serine 0.000002%, aspartic acid 0.00003%, proline 0.00002%, glycine 0.0006%, threonine 0.00002%, valine 0.00002%, arginine hydrochloride 0.00001%, tyrosine 0.000004%, alanine 0.0006%).

[0073] Vitamins (calcium pantothenate 0.00002%, vitamin B2 0.000004%, D-biotin 0.000016%, vitamin B12 0.00004%, folic acid 0.000004%, niacinamide 0.000004%, vitamin B6 0.000006%, vitamin B10.000004%, alpha-lipoic acid 0.000002%).

[0074] Example 4

[0075] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0076] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0077] Amino acids (glutamic acid 0.02%, glutamine 0.02%, histidine 0.002%, isoleucine 0.002%, leucine 0.002%, lysine hydrochloride 0.02%, methionine 0.002%, phenylalanine 0.002%, serine 0.002%, aspartic acid 0.02%, proline 0.02%, glycine 0.006%, threonine 0.02%, valine 0.02%, arginine hydrochloride 0.02%, tyrosine 0.002%, alanine 0.02%).

[0078] Vitamins (calcium pantothenate 0.005%, vitamin B2 0.01%, D-biotin 0.035%, vitamin B12 0.03%, folic acid 0.005%, niacinamide 0.005%, vitamin B6 0.003%, vitamin B1 0.002%, alpha-lipoic acid 0.005%).

[0079] Example 5

[0080] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0081] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0082] Amino acids (glutamic acid 0.002%, glutamine 0.002%, histidine 0.0002%, isoleucine 0.0002%, leucine 0.0002%, lysine hydrochloride 0.002%, methionine 0.0002%, phenylalanine 0.0002%, serine 0.0002%, aspartic acid 0.002%, proline 0.002%, glycine 0.0006%, threonine 0.002%, valine 0.002%, arginine hydrochloride 0.002%, tyrosine 0.0002%, alanine 0.002%).

[0083] Vitamins (calcium pantothenate 0.0075%, vitamin B2 0.015%, D-biotin 0.0525%, vitamin B12 0.045%, folic acid 0.0075%, niacinamide 0.0075%, vitamin B6 0.0045%, vitamin B1 0.003%, alpha-lipoic acid 0.0075%).

[0084] Example 6

[0085] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0086] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0087] Amino acids (glutamic acid 0.00253%, glutamine 0.00253%, histidine 0.0029%, isoleucine 0.0025%, leucine 0.002504%, lysine hydrochloride 0.00253%, methionine 0.0029%, phenylalanine 0.0033%, serine 0.002502%, aspartic acid 0.00253%, proline 0.00002%, glycine 0.0031%, threonine 0.00252%, valine 0.00252%, arginine hydrochloride 0.00251%, tyrosine 0.002504%, alanine 0.0031%).

[0088] Vitamins (calcium pantothenate 0.00011%, vitamin B2 0.000012%, D-biotin 0.000048%, vitamin B12 0.00012%, folic acid 0.000012%, niacinamide 0.000012%, vitamin B6 0.000018%, vitamin B10.000012%, alpha-lipoic acid 0.000006%).

[0089] Example 7

[0090] A skin care composition, the raw material composition of which is shown in Table 1, and the preparation method thereof is consistent with that of Example 1.

[0091] Buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%).

[0092] Amino acids (glutamic acid 0.02%, glutamine 0.02%, histidine 0.002%, isoleucine 0.002%, leucine 0.002%, lysine hydrochloride 0.01%, methionine 0.002%, phenylalanine 0.002%, serine 0.002%, aspartic acid 0.01%, proline 0.02%, glycine 0.006%, threonine 0.02%, valine 0.01%, arginine hydrochloride 0.02%, tyrosine 0.002%, alanine 0.02%).

[0093] Vitamins (calcium pantothenate 0.0025%, vitamin B2 0.005%, D-biotin 0.0175%, vitamin B12 0.015%, folic acid 0.0025%, niacinamide 0.0025%, vitamin B6 0.0015%, vitamin B1 0.001%, alpha-lipoic acid 0.0025%).

[0094] Table 1 Formulations of the skin care compositions of Examples 1-6

[0095]

[0096] Note: The amounts in Table 1 are in mass percentage.

[0097] Comparative Example 1

[0098] A skin care composition, the raw material composition of which is as follows: in terms of mass percentage, the content of buffer solute (4-hydroxyethylpiperazineethanesulfonic acid 0.03%, sodium 2-hydroxypyruvate 0.01%) is 0.04%, the content of amino acids (glutamic acid 0.04%, glutamine 0.04%, histidine 0.004%, isoleucine 0.004%, leucine 0.004%, lysine hydrochloride 0.04%, methionine 0.004%, phenylalanine 0.004%, serine 0.004%, aspartic acid 0.04%, proline 0.04%, glycine 0.012%, threonine 0.04%, valine 0.04%, arginine hydrochloride 0.04%, tyrosine 0.004%, alanine 0.04%) is 0.4%, and the content of vitamins (calcium pantothenate 0.00001%, vitamin B2 The content of 0.000001%, D-biotin 0.000004%, vitamin B12 0.00001%, folic acid 0.000001%, niacinamide 0.000001%, vitamin B6 0.000001%, vitamin B1 0.000001%, α-lipoic acid 0.000001%) is 0.00003%, the content of ectoine is 6.5%, and the content of sodium hyaluronate (molecular weight is 230W Da) is 0.005%. Its preparation method is consistent with that of Example 1.

[0099] Comparative Example 2

[0100] A skin care composition, which differs from Example 6 in that it does not contain amino acids, and the remaining steps are completely the same as those of Example 6.

[0101] Comparative Example 3

[0102] A skin care composition, which differs from Example 6 in that it does not contain vitamins, and the remaining steps are completely the same as those of Example 6.

[0103] Comparative Example 4

[0104] A skin care composition, which differs from Example 6 in that it does not contain ectoine, and the remaining steps are completely consistent with Example 6.

[0105] Comparative Example 5

[0106] A skin care composition, which is different from Example 6 in that water is used to replace α-lipoic acid, and the other components are completely consistent with those of Example 6, and the preparation method is completely consistent with that of Example 6.

[0107] Comparative Example 6

[0108] A skin care composition, which differs from Example 6 in that the ratio of various amino acids remains unchanged, the total amino acid content is 2.043%, and it does not contain ectoine. The remaining components are exactly the same as those in Example 6, and the preparation method is exactly the same as that in Example 6.

[0109] Comparative Example 7

[0110] A skin care composition, which differs from Example 6 in that it does not contain amino acids, the content of ectoine is 2.043%, the remaining components are exactly the same as those in Example 6, and the preparation method is exactly the same as that in Example 6.

[0111] Comparative Example 8

[0112] A skin care composition, which differs from Example 6 in that it does not contain α-lipoic acid, the ratio of various amino acids remains unchanged, the total amino acid content is 0.043006%, the remaining components are completely consistent with those of Example 6, and the preparation method is completely consistent with that of Example 6.

[0113] Comparative Example 9

[0114] A skin care composition, which differs from Example 6 in that: the content of α-lipoic acid is 0.1%, and it does not contain amino acids and ectoine; except water, the other components are completely consistent with those of Example 6, and the preparation method is completely consistent with that of Example 6.

[0115] Comparative Example 10

[0116] A skin care composition, which differs from Example 6 in that it does not contain α-lipoic acid and ectoine, the ratio of various amino acids remains unchanged, the total amino acid content is 2.043006%, the remaining components are completely consistent with those of Example 6, and the preparation method is completely consistent with that of Example 6.

[0117] Comparative Example 11

[0118] A skin care composition, which differs from Example 6 in that it does not contain α-lipoic acid and amino acid, the content of ectoine is 2.043006%, the remaining components are completely consistent with those of Example 6, and the preparation method is completely consistent with that of Example 6.

[0119] Example 8

[0120] A skin care composition, the raw material composition of which is shown in Table 2, and the preparation method thereof is as follows:

[0121] Dissolve the buffer solute in water for injection and filter to obtain solution A;

[0122] Add amino acids, vitamins, inorganic salts and coenzymes into solution A and dissolve them to obtain solution B;

[0123] The sugar substance is added into solution B, dissolved, and sterilized by wet heat to obtain a skin care composition.

[0124] Example 9

[0125] A skin care composition, the raw material composition of which is shown in Table 2, and the preparation method thereof is consistent with that of Example 8.

[0126] Example 10

[0127] A skin care composition, the raw material composition of which is shown in Table 2, and the preparation method thereof is consistent with that of Example 8.

[0128] Embodiment 11

[0129] A skin care composition, the raw material composition of which is shown in Table 2, and the preparation method thereof is consistent with that of Example 8.

[0130] Table 2 Formulations of the skin care compositions of Examples 8-11

[0131]

[0132]

[0133]

[0134] Comparative Example 12

[0135] A skin care composition, the raw material composition of which is shown in Table 3, and the preparation method thereof is consistent with that of Example 8.

[0136] Comparative Example 13

[0137] A skin care composition, the raw material composition of which is shown in Table 3, and the preparation method thereof is consistent with that of Example 8.

[0138] Comparative Example 14

[0139] A skin care composition, the raw material composition of which is shown in Table 3, and the preparation method thereof is consistent with that of Example 8.

[0140] Comparative Example 15

[0141] A skin care composition, the raw material composition of which is shown in Table 3, and the preparation method thereof is consistent with that of Example 8.

[0142] Comparative Example 16

[0143] A skin care composition, the raw material composition of which is shown in Table 3, and the preparation method thereof is consistent with that of Example 8.

[0144] Table 3 Formulations of the skin care compositions of Comparative Examples 12-16

[0145]

[0146]

[0147]

[0148] Experimental Example 1 Evaluation of anti-inflammatory effect

[0149] Hacat cells that have been revived and cultured for more than three generations are plated in a 6-well plate. After the cells adhere to the wall, the culture medium is discarded, 1 mL of PBS solution is added, and the cells are stimulated with LPS for 24 hours. Then, the formula (each example and comparative example) and blank culture medium are added to the wells in triplicate. After culturing in an incubator for 48 hours, the 6-well plate is taken out, washed with PBS, and 1 mL of TRIzol solution is added to each well to lyse the cells. Cell RNA is extracted in a nuclease-free environment according to the TRIzol standard process. After quantifying the RNA extracted in the above step, 1 μg is taken and reversed into cDNA using a reverse transcription kit, and qPCR amplification is performed using specific primers in the table below to obtain the Ct value, and the Ct value is calculated according to 2 -△△T The results are shown in Tables 4 and 5.

[0150]

[0151] Table 4 Evaluation of anti-inflammatory effects of Examples 1-7 and Comparative Examples 1-11

[0152]

[0153]

[0154] Analysis of the data in Table 4 revealed that the inflammatory factors in the Example group were significantly lower than those in the LPS modeling group, indicating that the skin care composition of the present invention has a better inhibitory effect on inflammatory factors.

[0155] Table 5 Evaluation of anti-inflammatory effects of Examples 8-11 and Comparative Examples 12-16

[0156] Grouping IL-6 TNF-a IL-1β Example 8 4.166 8.312 5.022 Example 9 4.231 8.347 5.311 Example 10 3.989 7.665 4.217 Embodiment 11 3.952 7.596 4.139 Comparative Example 12 5.021 9.852 8.995 Comparative Example 13 5.308 9.879 8.795 Comparative Example 14 5.214 9.732 8.669 Comparative Example 15 5.004 9.865 8.443 Comparative Example 16 5.032 10.021 8.657 Model Group 5.432 10.389 9.132

[0157] Analysis of the data in Table 5 revealed that the inflammatory factors in the Example group were significantly lower than those in the LPS modeling group, indicating that the skin care composition of the present invention has a better inhibitory effect on inflammatory factors.

[0158] Experimental Example 2 Evaluation of Antioxidant Effect

[0159] (1) Determination of superoxide dismutase and catalase activity

[0160] Hacat cells were counted and inoculated into 6-well plates. The cells were divided into a control group, a model group, and a model administration group. After being placed in an incubator for 12 hours, the culture medium containing the formula (each embodiment and comparative example) was replaced. The control group and the model group were culture medium without the formula. After 24 hours of culture, the culture medium containing H2O2 was replaced, and the culture was continued for 2 hours. The control group was a culture medium without H2O2. The superoxide dismutase (SOD) activity and catalase (CAT) activity of each group were determined using a kit, and the relative activity was calculated according to the formula: relative activity (%) = average OD value of the drug-addition well / average OD value of the control well × 100%. The results are shown in Tables 6, 7, 8, and 9.

[0161] Table 6 Statistics of superoxide dismutase activity of Examples 1-7 and Comparative Examples 1-11

[0162]

[0163] Table 7 Statistics of superoxide dismutase activity of Examples 8-11 and Comparative Examples 12-16

[0164]

[0165] Table 8 Statistics of catalase activity of Examples 1-7 and Comparative Examples 1-11

[0166]

[0167]

[0168] Table 9 Statistics of catalase activity of Examples 8-11 and Comparative Examples 12-16

[0169]

[0170] It can be seen from the data in Tables 6-9 that the skin care composition of the present application can significantly increase the activity of superoxide dismutase and catalase and has a certain antioxidant effect.

[0171] Experimental Example 1 Evaluation of Whitening Effect

[0172] Take out the cryopreserved tube containing B16F10 cells from liquid nitrogen, place it in a 37°C constant temperature water bath to thaw, place it in a centrifuge, discard the supernatant, resuspend the cell mass with complete medium, plant it in a cell culture dish and add an appropriate amount of complete medium to mix, place it in a cell culture incubator for overnight culture and replace with fresh medium. Use an inverted microscope to observe the cells until they grow to 80-90% confluence, aspirate the medium, wash twice with PBS, add an appropriate amount of 0.25% trypsin, place it in an incubator for digestion for an appropriate time, wait for the cells to become round and bright under the microscope, add complete medium to stop digestion, and transfer it to a 10mL centrifuge tube by blowing, centrifuge at 1000rpm for 5min, discard the supernatant and resuspend it in a new culture dish with complete medium, and continue to culture in an incubator.

[0173] (1) Determination of melanin content by sodium hydroxide lysis method

[0174] B16F10 cells were digested and resuspended, counted, inoculated into 6-well plates, placed in an incubator for 12 hours, washed once with PBS, and replaced with a medium containing the formula (each embodiment and comparative example), and the control group was a medium without the formula. After continuing to culture for 24 hours, the supernatant was discarded, washed with PBS, digested with trypsin, and the cells were collected in a centrifuge tube and centrifuged for 10 minutes, the supernatant was discarded, 1 mL of 1 mol / L NaOH solution containing 10% DMSO was added, and after 1 hour of water bath at 80°C, they were respectively transferred into 96-well culture plates, and the absorbance value was detected in an ELISA instrument, 405 nm was set as the detection wavelength, and the absorbance value (OD value) was read, and the content of intracellular melanin was calculated according to the formula. Relative content of melanin (%) = average OD value of drug-added wells / average OD value of control wells × 100%.

[0175] The results are shown in Tables 10 and 11.

[0176] (2) Determination of tyrosinase activity

[0177] In a 96-well plate, L-DOPA was used as the substrate and PBS was used as the buffer. The substrate solution was added, and then the same volume of the formula (each embodiment and comparative example) solution was added. After incubation at 37°C for 5 minutes, the absorbance at 475nm was measured. Finally, tyrosinase (TYR) solution was added, incubated at 37°C for 15 minutes, and the absorbance at 475nm was measured again. The control group used PBS solution instead of the formula solution. The TYR inhibition rate was calculated according to the following formula:

[0178]

[0179] Where: R TYRis the TYR inhibition rate (%); A1 and A2 are the absorbance values ​​of the non-drug group and the drug group after 5 minutes of pre-culture; A1' and A2' are the absorbance values ​​of the two groups after 15 minutes of culture. The results are shown in Tables 12 and 13.

[0180] Table 10 Statistics on the effects of the products of Examples 1-7 and Comparative Examples 1-11 on melanin content

[0181] Grouping Melanin relative content (%) Example 1 76.82 Example 2 78.95 Example 3 72.36 Example 4 73.88 Example 5 75.41 Example 6 70.46 Example 7 71.62 Comparative Example 1 86.74 Comparative Example 2 92.33 Comparative Example 3 94.57 Comparative Example 4 94.97 Comparative Example 5 89.43 Comparative Example 6 88.02 Comparative Example 7 90.33 Comparative Example 8 91.47 Comparative Example 9 92.59 Comparative Example 10 89.78 Comparative Example 11 90.24

[0182] Table 11 Statistics on the effects of the products of Examples 8-11 and Comparative Examples 12-16 on melanin content

[0183]

[0184]

[0185] Table 12 Statistics of the effects of the products of Examples 1-7 and Comparative Examples 1-11 on tyrosinase activity

[0186] Grouping Tyrosinase activity inhibition rate (%) Example 1 27.80 Example 2 25.23 Example 3 29.38 Example 4 30.04 Example 5 26.54 Example 6 38.66 Example 7 40.39 Comparative Example 1 11.69 Comparative Example 2 2.36 Comparative Example 3 1.57 Comparative Example 4 2.08 Comparative Example 5 10.02 Comparative Example 6 9.87 Comparative Example 7 10.16 Comparative Example 8 9.74 Comparative Example 9 9.26 Comparative Example 10 5.67 Comparative Example 11 4.85

[0187] Table 13 Statistics on the effects of the products of Examples 8-11 and Comparative Examples 12-16 on tyrosinase activity

[0188]

[0189]

[0190] It can be seen from the data in Tables 10-13 that the skin care composition of the present application can significantly reduce the melanin content, inhibit the activity of tyrosinase, and has a significant whitening effect.

[0191] From the comparison of the effects of Examples 1-7 and Comparative Examples 1-11, it can be seen that the added vitamin substances, α-lipoic acid, amino acid substances and ectoine play a synergistic role, and the whitening, anti-inflammatory and antioxidant effects are more obvious than those of α-lipoic acid and amino acid substances, α-lipoic acid and ectoine, and ectoine and amino acid substances.

[0192] From the comparison of the effects of Examples 8-11 and Comparative Examples 12-16, it can be seen that the added adenine sulfate in the vitamin substances and the cystine, tryptophan and asparagine in the amino acid substances play a synergistic role, and the whitening, anti-inflammatory and antioxidant effects are more obvious than not adding adenine sulfate, not adding cystine, tryptophan and asparagine, adenine sulfate only used together with cystine, adenine sulfate only used together with tryptophan, and adenine sulfate only used together with aspartic acid.

[0193] In summary, the composition of the present invention can play an obvious whitening, anti-oxidation and anti-inflammatory effect.

[0194] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A skin care composition, characterized in that Calculated by mass percentage, it includes: 0.01-5% hyaluronic acid and / or its salt, 0.01-6% ectoine and / or its derivatives, 0.00005-0.2% vitamins, 0.01-0.3% amino acids, 0.01-0.04% buffer solutes, and the balance is water.

2. The skin care composition according to claim 1, characterized in that The molecular weight of the hyaluronic acid and / or its salt is 5000-200W Da, preferably 50W-100W Da; Preferably, the hyaluronate is at least one selected from sodium hyaluronate, zinc hyaluronate, potassium hyaluronate, magnesium hyaluronate and calcium hyaluronate, preferably sodium hyaluronate; Preferably, the ectoine derivative is one of hydroxyectoine, ectoine sodium salt and ectoine potassium salt; Preferably, the vitamin is selected from at least one of vitamin B and vitamin C, and the vitamin B is selected from at least one of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, nicotinamide, and α-lipoic acid; preferably, the vitamin is a mixture of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, nicotinamide, and α-lipoic acid; Preferably, the amino acid is selected from at least one of glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine, and alanine; Preferably, the buffer solute is selected from at least one of 4-hydroxyethylpiperazineethane sulfonic acid and sodium 2-hydroxypyruvate; further preferably, in terms of mass percentage, the content of 4-hydroxyethylpiperazineethane sulfonic acid is 0.03%, and the content of sodium 2-hydroxypyruvate is 0.01%.

3. The skin care composition according to claim 1, characterized in that The skin care composition comprises, in percentage by mass: 0.05-3% hyaluronic acid and / or its salt, 0.05-5% ectoine and / or its derivative, 0.0001-0.1% vitamin, 0.03-0.2% amino acid, 0.01-0.04% buffer solute, and the balance is water; Preferably, the skin care composition comprises, by mass percentage: 0.05-3% sodium hyaluronate, 0.05-5% ectoine, 0.0001-0.1% vitamins, 0.03-0.2% amino acids, 0.04% buffer solutes, and the balance is water, and the molecular weight of the sodium hyaluronate is 50W-100W Da; Preferably, the skin care composition comprises, by mass percentage, 2% sodium hyaluronate, 2% ectoine, 0.00035% vitamins, 0.043% amino acids, 0.04% buffer solutes, and the balance is water. The molecular weight of the sodium hyaluronate is 80W Da.

4. A method for preparing the skin care composition according to any one of claims 1 to 3, characterized in that: The steps include: Dissolve the buffer solute in water to obtain solution A; Adding ectoine and / or its derivatives, vitamins and amino acids to solution A to obtain solution B; adding hyaluronic acid and / or its salt into solution B to obtain a skin care composition; Preferably, after adding hyaluronic acid and / or its salt to solution B, sterilization treatment is performed to obtain a skin care composition; preferably, the sterilization treatment includes filtration sterilization, ethylene oxide sterilization, irradiation sterilization, moist heat sterilization, preferably moist heat sterilization.

5. Use of the skin care composition according to any one of 1 to 3 in preparing a product having whitening, anti-inflammatory and antioxidant effects; Preferably, the dosage form of the product is a solution, a gel, a spray, drops or a lotion, preferably a gel; Preferably, the product is a cosmetic, a medical device or a medicine; Preferably, the product is a medical device.

6. A skin care composition, characterized in that Calculated by mass percentage, it includes: 0.1-5% carbohydrates, 0.000001-0.0001% vitamins, 0.00001-0.1% amino acids, 0.01-0.1% inorganic salts, 0.000001-0.0005% coenzymes, and the balance is water.

7. The skin care composition according to claim 5, characterized in that Also included are buffer solutes; Preferably, the buffer solute is at least one of 4-hydroxyethylpiperazineethanesulfonic acid and sodium 2-hydroxypyruvate; further preferably, in terms of mass percentage, the content of 4-hydroxyethylpiperazineethanesulfonic acid is 0.03%, and the content of sodium 2-hydroxypyruvate is 0.01%; Preferably, the carbohydrate substance is selected from at least one of glucose, chitosan, hyaluronic acid and / or hyaluronate, and sodium carboxymethyl cellulose; preferably, the carbohydrate substance is hyaluronic acid and / or hyaluronate; Preferably, the hyaluronate is at least one of sodium hyaluronate, zinc hyaluronate, potassium hyaluronate, magnesium hyaluronate and calcium hyaluronate; preferably, the hyaluronate is sodium hyaluronate; Preferably, the molecular weight of the hyaluronic acid and / or hyaluronate is 5000-200 WDa, preferably 50W-100 WDa; Preferably, the vitamin is selected from at least one of vitamin B, vitamin C, and adenine sulfate; wherein the vitamin B is selected from at least one of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, vitamin B12, biotin, nicotinamide, and α-lipoic acid; preferably, the vitamin is a mixture of vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin B9, vitamin B12, adenine sulfate, biotin, nicotinamide, and α-lipoic acid; Preferably, the amino acid is selected from at least one of arginine, lysine, histidine, aspartic acid, glutamic acid, glycine, alanine, valine, leucine, isoleucine, phenylalanine, proline, tryptophan, serine, tyrosine, cysteine, methionine, asparagine, glutamine and threonine; Preferably, the inorganic salt is selected from at least one of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium carbonate, sodium bicarbonate, citric acid, sodium citrate, and sodium metasilicate; preferably, the inorganic salt is a mixture of disodium hydrogen phosphate, sodium chloride, and sodium metasilicate; Preferably, the coenzyme is α-lipoic acid.

8. The skin care composition according to claim 5, characterized in that In terms of mass percentage, the skin care composition comprises: 0.05-3% carbohydrate, 0.00001-0.00005% vitamin, 0.0001-0.001% amino acid, 0.03-0.08% inorganic salt, 0.00001-0.00005% coenzyme, and the balance is water; Preferably, the skin care composition comprises, by mass percentage: 0.05-3% carbohydrate, 0.00001-0.00005% vitamin, 0.0001-0.001% amino acid, 0.03-0.08% inorganic salt, 0.00001-0.00005% coenzyme, and the balance is water; the carbohydrate is sodium hyaluronate and glucose; the vitamin is vitamin B5, vitamin B2, biotin, vitamin B12, phyllotoxin, vitamin B6, vitamin B7, vitamin B8, vitamin B9, vitamin B10, vitamin B11, vitamin B12, vitamin B13, vitamin B14, vitamin B15, vitamin B16, vitamin B17, vitamin B18, vitamin B19, vitamin B12, vitamin B12, vitamin B19, vitamin B12, vitamin B13, vitamin B19, vitamin B12, vitamin B13, vitamin B19, vitamin B1 The invention relates to a novel nanostructured protein, comprising nicotinic acid, amine, vitamin B6, vitamin B1 and adenine sulfate; the amino acids are aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salts are disodium hydrogen phosphate, potassium chloride and sodium metasilicate; the coenzyme is α-lipoic acid; Preferably, in terms of mass percentage, the skin care composition comprises: 0.05% carbohydrate, 0.00001% vitamin, 0.0001% amino acid, 0.03% inorganic salt, 0.00001% coenzyme, and the balance is water; the carbohydrate is sodium hyaluronate and glucose; the vitamin is composed of vitamin B5, vitamin B2, biotin, vitamin B12, folic acid, niacinamide, vitamin B6, vitamin B1 and adenine sulfate; the amino acid is composed of aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salt is composed of disodium hydrogen phosphate, potassium chloride and sodium metasilicate; the coenzyme is α-lipoic acid; Preferably, in terms of mass percentage, the skin care composition comprises: 3% carbohydrates, 0.00005% vitamins, 0.001% amino acids, 0.08% inorganic salts, 0.00005% coenzymes, and the balance is water; the carbohydrates are sodium hyaluronate and glucose; the vitamins are composed of vitamin B5, vitamin B2, biotin, vitamin B12, folic acid, niacinamide, vitamin B6, vitamin B1 and adenine sulfate; the amino acids are composed of aspartic acid, cystine, glutamic acid, glutamine, histidine, isoleucine, leucine, lysine hydrochloride, methionine, phenylalanine, serine, tryptophan, asparagine, proline, glycine, threonine, valine, arginine hydrochloride, tyrosine and alanine; the inorganic salts are composed of disodium hydrogen phosphate, potassium chloride and sodium metasilicate; and the coenzyme is α-lipoic acid.

9. A method for preparing the skin care composition according to any one of claims 5 to 7, characterized in that: The steps include: Dissolve vitamins, amino acids, inorganic salts and coenzymes in water to obtain solution C; Adding a sugar substance to solution C to obtain a skin care composition; Preferably, after adding the sugar substance to solution C, sterilization treatment is performed to obtain a skin care composition; preferably, the sterilization treatment includes filtration sterilization, ethylene oxide sterilization, irradiation sterilization, moist heat sterilization, preferably moist heat sterilization; Preferably, the preparation method comprises dissolving the auxiliary material in water, and then adding vitamins, amino acids, inorganic salts and coenzymes to obtain solution C.

10. Use of the skin care composition according to any one of 6 to 8 in preparing a product having whitening, anti-inflammatory and antioxidant effects; Preferably, the dosage form of the product is a solution, a gel, a spray, drops or a lotion, preferably a gel; Preferably, the product is a cosmetic, a medical device or a medicine; Preferably, the product is a medical device.

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