Application of compound agilawood extract in preparation of foot bath bag

Abstract

The invention provides an application of a compound agilawood extract in preparation of a foot bath bag, the compound agilawood extract comprises an agilawood extract, a sea grape extract and an eclipta extract in a mass ratio of (3-6): 1: (2-4), and the foot bath bag can be used as a skin roughness inhibitor and a diarrhea ameliorant. Compared with the prior art, the aquilaria wood extract, the sea grape extract and the herba ecliptae extract are creatively combined and applied to the foot bath bag to serve as a skin roughness inhibitor for enhancing the expression of caspase 14 and a diarrhea improving agent for enhancing the expression of sucrase-isomaltase, and the effects of well removing cutin and improving diarrhea can be achieved. And the traditional Chinese medicine composition can inhibit rough skin and relieve uncomfortable symptoms such as diarrhea, and has a wide application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, and in particular to application of a compound agarwood extract in preparing a foot bath bag. Background Art

[0002] With the development of society and the accelerated pace of life, the pressure from society, work, life, etc. is increasing. When the human body's immunity is low and cannot resist the counterattack of external factors, the physiological function will lose balance and cause diseases. Too much unabsorbed sucrose accumulates in the intestinal cavity, causing the intestinal cavity osmotic pressure to be too high, and the sucrose ferments in the intestinal cavity, and finally forms typical acidic watery stools or pasty stools. When the intake of sucrose is high, the stool volume is large and sour, which can cause severe diarrhea in infancy. Children, adolescents and adults generally have mild symptoms, which may only manifest as intermittent abdominal discomfort, increased frequency of bowel movements, thin stools, sour and smelly stools, etc. Nowadays, improper eating, greasy diet, increased stress and other factors can cause digestive disorders, which are mainly manifested as an increase in bowel movements. Depending on the severity, it can be divided into acute and chronic diarrhea.

[0003] Chinese medicine foot bath therapy is based on traditional Chinese medicine theory. It uses Chinese medicine for external foot bath. Through the absorption of the skin and mucous membranes and the warm fumigation of the medicine, the drug ions quickly enter the blood, allowing the medicinal properties to reach the internal organs directly through the acupoints, stimulate the acupoints throughout the body, dredge the meridians, and regulate qi and blood. Chinese medicine theory believes that by stimulating these acupoints, the effects of dredging the meridians, regulating qi and blood, and regulating the functions of the internal organs can be achieved.

[0004] Therefore, it is necessary to develop a foot bath bag with low cost, which can not only remove dead skin, make the sole skin smooth and tender, and inhibit skin roughness, but also relieve diarrhea, which has practical significance. Summary of the invention

[0005] In view of this, the present invention proposes the use of a compound agarwood extract in the preparation of a foot bath bag.

[0006] The technical solution of the present invention is achieved in this way:

[0007] Application of compound agarwood extract in preparing foot bath bag.

[0008] Furthermore, the compound agarwood extract comprises agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 3-6:1:2-4.

[0009] Furthermore, the foot bath bag serves as a rough skin inhibitor and diarrhea improver.

[0010] Furthermore, the preparation method of the agarwood extract comprises:

[0011] (1) freezing and withering fresh agarwood leaves, adding them to an ethanol solution for ultrasonic extraction, and obtaining an extract;

[0012] (2) adding the filter residue to a composite enzyme for enzymatic hydrolysis, combining the enzymatic hydrolysis solution with the extract of step (1), and concentrating under reduced pressure to obtain an agarwood extract.

[0013] Furthermore, in step (1), the freezing temperature is -20 to -10°C, and the time is 2-4h; the withering temperature is 20-25°C, and the thawing time is 1-2h; the concentration of the ethanol solution is 40%-60% v / v; the solid-liquid ratio of the agarwood leaves to the ethanol solution is 1:6-10g / mL; and the ultrasonic extraction is performed at 20-40kHz and 60-80°C for 30-60min.

[0014] Furthermore, in step (2), the mass ratio of the filter residue to the complex enzyme is 1:0.1-0.3; the complex enzyme is β-glucosidase and cellulase in a mass ratio of 1:3-5, and the enzyme activity is 80-100 IU / mL; the enzymatic hydrolysis time is 1-3h, and the temperature is 30-35°C.

[0015] Furthermore, the preparation method of the sea grape extract comprises: crushing the sea grapes and adding them into water, performing microwave reflux extraction, collecting the extract, and concentrating under reduced pressure to obtain the sea grape extract.

[0016] Furthermore, the solid-liquid ratio of the sea grape to water is 1:10-15 g / mL; the number of microwave reflux extraction is 1-3 times, each time for 30-60 minutes, the temperature is 50-70° C., and the power is 400-600W.

[0017] Furthermore, the preparation method of the Eclipta prostrata extract comprises: drying fresh Eclipta prostrata, ultrafine grinding to obtain ultrafine powder, adding 40%-60% v / v ethanol solution for extraction, passing the extract through an ultrafiltration membrane with a molecular weight cutoff of 1kDa-3kDa, and concentrating the retentate under reduced pressure to obtain the Eclipta prostrata extract.

[0018] Furthermore, the particle size of the ultrafine powder is 50-80 μm; the solid-liquid ratio of the ultrafine powder to the ethanol solution is 1:4-6 g / mL; the extraction temperature is 60-80° C., and the extraction time is 2-4 hours.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] Compared with the prior art, the present invention creatively applies a combination of agarwood extract, sea grape extract and Eclipta prostrata extract to a foot bath bag as a skin roughness inhibitor that enhances the expression of caspase 14 and a diarrhea improver that enhances the expression of sucrase-isomaltase, which can achieve a good effect of removing dead skin and smoothing the skin, inhibit skin roughness, and relieve diarrhea and other uncomfortable symptoms. DETAILED DESCRIPTION

[0021] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0022] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0023] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0024] Preparation of the foot bath bag of the present invention: The compound agarwood extract is placed in a non-woven inner packaging bag and sterilized by ultraviolet light (ultraviolet wavelength 250nm, intensity 80W / cm 2 ), and seal it in a plastic outer packaging bag to obtain a foot bath bag.

[0025] The composite agarwood extract of the present invention is obtained by uniformly mixing the agarwood extract, the sea grape extract and the Eclipta prostrata extract.

[0026] Example 1

[0027] The compound agarwood extract in the foot bath bag of this embodiment includes agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 5:1:3.

[0028] The preparation method of the agarwood extract of this embodiment includes:

[0029] (1) Fresh agarwood leaves were frozen (-15°C, 3h) and withered (23°C), thawed for 1.5h, and added to a 50% v / v ethanol solution at a solid-liquid ratio of 1:8 g / mL, and ultrasonically extracted (30kHz, 70°C) for 45min to obtain an extract;

[0030] (2) Add the filter residue to a composite enzyme (β-glucosidase and cellulase in a mass ratio of 1:4, with enzyme activities of 90 IU / mL), the mass ratio of the filter residue to the composite enzyme is 1:0.2, and enzymolysis is performed at 32° C. for 2 h. The enzymolysis solution is combined with the extract of step (1), and concentrated under reduced pressure to obtain an agarwood extract.

[0031] The preparation method of the sea grape extract of this embodiment includes:

[0032] According to the solid-liquid ratio of 1:13 g / mL, the sea grapes were crushed and added into water, and extracted twice by microwave reflux (60°C, 500 W), each time for 45 min. The extract was collected and concentrated under reduced pressure to obtain the sea grape extract.

[0033] The preparation method of the Eclipta prostrata extract of this embodiment comprises:

[0034] Fresh Eclipta prostrata was dried and ultrafinely ground to obtain ultrafine powder with a particle size of 70 μm. The ultrafine powder was added to a 50% v / v ethanol solution at a solid-liquid ratio of 1:5 g / mL, and extracted at 70°C for 3 h. The extract was passed through an ultrafiltration membrane with a molecular weight cutoff of 2 kDa, and the retentate was concentrated under reduced pressure to obtain the Eclipta prostrata extract.

[0035] Example 2

[0036] The compound agarwood extract of the foot bath bag of this embodiment includes agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 3:1:2.

[0037] The preparation method of the agarwood extract of this embodiment includes:

[0038] (1) Fresh agarwood leaves were frozen (-20°C, 2h) and withered (20°C), thawed for 1h, and then added to a 40% v / v ethanol solution at a solid-liquid ratio of 1:6 g / mL, and ultrasonically extracted (20kHz, 60°C) for 30 min to obtain an extract;

[0039] (2) Add the filter residue to a composite enzyme (β-glucosidase and cellulase in a mass ratio of 1:3, with enzyme activities of 80 IU / mL), the mass ratio of the filter residue to the composite enzyme is 1:0.1, and perform enzymolysis at 30°C for 1 hour. Combine the enzymolysis solution with the extract of step (1), and concentrate under reduced pressure to obtain an agarwood extract.

[0040] The preparation method of the sea grape extract of this embodiment includes:

[0041] According to the solid-liquid ratio of 1:10 g / mL, the sea grapes were crushed and added into water, and extracted once by microwave reflux (50°C, 400W) for 30 min each time. The extract was collected and concentrated under reduced pressure to obtain the sea grape extract.

[0042] The preparation method of the Eclipta prostrata extract of this embodiment comprises:

[0043] Fresh Eclipta prostrata was dried and ultrafinely ground to obtain ultrafine powder with a particle size of 50 μm. The ultrafine powder was added to a 40% v / v ethanol solution at a solid-liquid ratio of 1:4 g / mL, and extracted at 60°C for 2 h. The extract was passed through an ultrafiltration membrane with a molecular weight cutoff of 1 kDa, and the retentate was concentrated under reduced pressure to obtain the Eclipta prostrata extract.

[0044] Example 3

[0045] The compound agarwood extract in the foot bath bag of this embodiment includes agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 6:1:4.

[0046] The preparation method of the agarwood extract of this embodiment includes:

[0047] (1) The fresh agarwood leaves were frozen (-10°C, 4h) and withered (25°C), thawed for 2h, and then added to a 60% v / v ethanol solution at a solid-liquid ratio of 1:10 g / mL, and ultrasonically extracted (40 kHz, 80°C) for 60 min to obtain an extract;

[0048] (2) Add the filter residue to a composite enzyme (β-glucosidase and cellulase in a mass ratio of 1:5, with enzyme activities of 100 IU / mL), the mass ratio of the filter residue to the composite enzyme is 1:0.3, and enzymolysis is carried out at 35° C. for 3 h. The enzymolysis solution is combined with the extract of step (1), and concentrated under reduced pressure to obtain an agarwood extract.

[0049] The preparation method of the sea grape extract of this embodiment includes:

[0050] According to the solid-liquid ratio of 1:15 g / mL, the sea grapes were crushed and added into water, and extracted by microwave reflux (70°C, 600 W) for 3 times, each time for 60 min. The extract was collected and concentrated under reduced pressure to obtain the sea grape extract.

[0051] The preparation method of the Eclipta prostrata extract of this embodiment comprises:

[0052] Fresh Eclipta prostrata was dried and ultrafinely ground to obtain ultrafine powder with a particle size of 80 μm. The ultrafine powder was added to a 60% v / v ethanol solution at a solid-liquid ratio of 1:6 g / mL, and extracted at 80°C for 4 h. The extract was passed through an ultrafiltration membrane with a molecular weight cutoff of 3 kDa, and the retentate was concentrated under reduced pressure to obtain the Eclipta prostrata extract.

[0053] Comparative Example 1

[0054] The difference from Example 1 is that the compound agarwood extract includes agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 1:1:1, and the rest is consistent with Example 1.

[0055] Comparative Example 2

[0056] The difference from Example 1 is that the compound agarwood extract lacks sea grape extract, that is, the compound agarwood extract includes agarwood extract and Eclipta prostrata extract in a mass ratio of 5:3, and the rest is consistent with Example 1.

[0057] Comparative Example 3

[0058] The difference from Example 1 is that the compound agarwood extract lacks the Eclipta prostrata extract, that is, the compound agarwood extract includes the agarwood extract and the sea grape extract in a mass ratio of 5:1, and the rest is consistent with Example 1.

[0059] Comparative Example 4

[0060] The difference from Example 1 is that the compound agarwood extract is replaced by agarwood extract, and the rest is the same as Example 1.

[0061] Comparative Example 5

[0062] The difference from Example 1 is that the extraction methods of the agarwood extract, the sea grape extract and the Eclipta prostrata extract are all traditional alcohol extractions, and the rest are consistent with Example 1.

[0063] That is, the preparation method of the agarwood extract of this comparative example includes:

[0064] According to the solid-liquid ratio of 1:8 g / mL, fresh agarwood leaves were added to a 50% v / v ethanol solution and extracted by ultrasonic (30 kHz, 70°C) for 45 minutes to obtain an extract, which was concentrated under reduced pressure to obtain an agarwood extract.

[0065] The preparation method of the sea grape extract of this comparative example comprises:

[0066] According to the solid-liquid ratio of 1:13 g / mL, the sea grapes were crushed and added into a 50% v / v ethanol solution, and extracted by microwave reflux (60° C., 500 W) for 45 minutes. The extract was collected and concentrated under reduced pressure to obtain the sea grape extract.

[0067] The preparation method of the Eclipta prostrata extract of this comparative example comprises:

[0068] According to the solid-liquid ratio of 1:5g / mL, fresh Eclipta prostrata was dried and crushed, then added to a 50% v / v ethanol solution, extracted at 70°C for 3h, the extract was collected, and concentrated under reduced pressure to obtain the Eclipta prostrata extract.

[0069] Test Example 1

[0070] Experimental animals: 100 male mice (6 weeks old, weight 30±5 g) were fed for one week before the experiment. The feeding conditions were: 23±2°C, humidity 55±5%, lighting time 12h / day, free drinking water.

[0071] Modeling method: With reference to the experimental method of "Journal of Nantong University (Medical Edition) (2018, 38(2): 81-84)", DIBS animal model was prepared using senna leaves and restraint stress load to evaluate the biological activity of the test substance: Senna leaves (Sennaefolium) are small leaves of the leguminous plants Senna angustifolia or Senna acuminate, which are used in traditional medicine to relieve heat and stagnation, promote diuresis, heat accumulation, constipation, etc. In addition, the plant is rich in active ingredients such as chrysophanol and rhein and is also a stimulant laxative, which acts on the colon through the intestinal mucosa and nervous system to stimulate intestinal peristalsis; the present invention, on the basis of giving mice senna leaves water extract, uses restraint stress load as a psychological factor intervention to establish a DIBS animal model.

[0072] Grouping and administration method: 100 male mice were randomly divided into a normal group, a model group, an embodiment 1-3 group and a comparative example 1-5 group, with 10 mice in each group. The embodiment 1-3 group and the comparative example 1-5 group were given 0.15 g / kg of the compound agarwood composition prepared in embodiments 1-3 and comparative examples 1-5 by intragastric administration, and fasted for 12 hours after administration; the normal group and the model group were given an equal amount of physiological saline.

[0073] Then, the mice in the model group, Example 1-3 group and Comparative Example 1-5 group were intragastrically administered with 0.5 g / mL senna leaf aqueous solution at a dose of 10 mL / kg, and then placed in a 50 mL restraint tube for 2 hours. After the restraint was released, the defecation situation was observed within 6 hours.

[0074] The loose stool rate was calculated by using the filter paper blotting method to observe and calculate the total number of feces and loose stool particles of mice 6 hours after modeling. The loose stool rate analysis was based on whether there were stains on the filter paper.

[0075] Loose stool rate (%) = number of loose stool particles / total number of stool particles × 100%. The test results are shown in Table 1.

[0076] Table 1

[0077] sample Average loose stool rate / % Loose stool inhibition rate / % Normal group 0 - Model Group 25.2 - Example 1 18.4 26.98 Example 2 19.2 23.81 Example 3 18.8 25.40 Comparative Example 1 21.6 14.29 Comparative Example 2 22.7 9.92 Comparative Example 3 23.2 7.94 Comparative Example 4 24.4 3.17 Comparative Example 5 23.8 5.56

[0078] As can be seen from Table 1, the composite agarwood extracts prepared in Examples 1-3 of the present invention can effectively improve the diarrhea symptoms of mice. Compared with Example 1, the diarrhea inhibition effect of Comparative Examples 1-5 is reduced, indicating that the combination of the agarwood extract, sea grape extract and Eclipta prostrata extract of the present invention has synergistic effects. In addition, the individual extracts and proportions of the composite agarwood extract and the specific extraction method are key factors affecting the efficacy.

[0079] Test Example 2

[0080] Test subjects: 80 volunteers aged 20-60, divided into 8 groups, 10 people in each group.

[0081] Test method: After unpacking the plastic outer packaging bag of the foot bath bag prepared in Examples 1-3 of the present invention and Comparative Examples 1-5, put the non-woven inner packaging bag together with the medicine powder into the foot bath basin, add 2L of boiling water, stir and let stand for 10 minutes, then add cold water to adjust the water temperature to 45°C, soak the feet statically for 25 minutes, and add hot water at any time to keep the water temperature at 45°C. Soak the feet once a day for 7 consecutive days as a course of treatment, observe 4 courses in total, record the use experience, test the moisture of the foot skin after the course of treatment, observe and record the foot skin condition;

[0082] The test values ​​before and after use were compared and statistically analyzed to observe the change in moisture content and determine the exfoliating and moisturizing effects of the foot bath bag on the foot skin.

[0083] Effect judgment criteria:

[0084] Effective: The surface of the foot peels after use, and the surface becomes smoother after peeling;

[0085] Invalid: No effect.

[0086] The measurement results are shown in Table 2.

[0087] Table 2

[0088] sample Peeling effect Increased moisture Surface smoothness increases Allergies Exfoliating effect Example 1 100％ 15.4％ 55％ 0 95％ Example 2 100％ 14.5％ 50％ 0 90％ Example 3 100％ 15.1％ 53％ 0 92％ Comparative Example 1 100％ 11.4％ 37％ 0 87％ Comparative Example 2 95％ 9.7％ 31％ 0 79％ Comparative Example 3 90％ 8.8％ 28％ 0 77％ Comparative Example 4 85％ 5.4％ 15％ 0 70％ Comparative Example 5 88％ 5.7％ 22％ 0 83％

[0089] As shown in Table 2, the compound agarwood extracts of Examples 1-3 of the present invention can exfoliate, moisturize, solve the problem of rough skin, and will not cause skin allergies. Compared with Example 1, the effect of inhibiting rough foot skin in Comparative Examples 1-5 is reduced.

[0090] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. Application of compound agarwood extract in the preparation of foot bath bags.

2. The use according to claim 1, characterized in that The compound agarwood extract comprises agarwood extract, sea grape extract and Eclipta prostrata extract in a mass ratio of 3-6:1:2-4.

3. The use according to claim 1, characterized in that The foot bath bag acts as a rough skin inhibitor and diarrhea improver.

4. The use according to claim 2, characterized in that The preparation method of the agarwood extract comprises: (1) freezing and withering fresh agarwood leaves, adding them to an ethanol solution for ultrasonic extraction, and obtaining an extract; (2) adding the filter residue to a composite enzyme for enzymatic hydrolysis, combining the enzymatic hydrolysis solution with the extract of step (1), and concentrating under reduced pressure to obtain an agarwood extract.

5. The use according to claim 4, characterized in that In step (1), the freezing temperature is -20 to -10°C, and the time is 2-4h; the withering temperature is 20-25°C, and the thawing time is 1-2h; the concentration of the ethanol solution is 40%-60% v / v; the solid-liquid ratio of the agarwood leaves to the ethanol solution is 1:6-10g / mL; and the ultrasonic extraction is performed at 20-40kHz and 60-80°C for 30-60min.

6. The use according to claim 4, characterized in that In step (2), the mass ratio of the filter residue to the complex enzyme is 1:0.1-0.3; the complex enzyme is β-glucosidase and cellulase in a mass ratio of 1:3-5, and the enzyme activity is 80-100 IU / mL; the enzymatic hydrolysis time is 1-3h, and the temperature is 30-35°C.

7. The use according to claim 2, characterized in that The preparation method of the sea grape extract comprises: crushing the sea grapes and adding them into water, performing microwave reflux extraction, collecting the extract, and concentrating under reduced pressure to obtain the sea grape extract.

8. The use according to claim 7, characterized in that The material-liquid ratio of the sea grape to water is 1:10-15 g / mL; the microwave reflux extraction is performed 1-3 times, each time for 30-60 minutes, at a temperature of 50-70° C. and a power of 400-600W.

9. The use according to claim 2, characterized in that The preparation method of the Eclipta prostrata extract comprises: drying fresh Eclipta prostrata, ultrafine grinding to obtain ultrafine powder, adding 40%-60% v / v ethanol solution for extraction, passing the extract through an ultrafiltration membrane with a molecular weight cutoff of 1kDa-3kDa, and concentrating the retentate under reduced pressure to obtain the Eclipta prostrata extract.

10. The use according to claim 9, characterized in that The particle size of the ultrafine powder is 50-80 μm; the solid-liquid ratio of the ultrafine powder to the ethanol solution is 1:4-6 g / mL; the extraction temperature is 60-80° C. and the extraction time is 2-4 hours.