Plant acne-removing composition and preparation method thereof

Abstract

The invention discloses a plant acne-removing composition and a preparation method thereof. The composition is prepared by compounding salvia miltiorrhiza, common leafflower herb, aloe, green tea, gentiana macrophylla, snail mucus and the like. The active ingredients of the plant acne-removing composition are compatible by adopting extracts of natural plants, the combination of the radix salviae miltiorrhizae extract, the common leafflower herb extract, the aloe extract, the green tea extract, the large-leaved gentian extract and the snail mucus is natural and non-toxic, the acne-removing effect is good, the effect taking speed is high, acne marks are not left, all the extracts have a synergistic effect, and the plant acne-removing composition is free of toxic and side effects. The effect of treating acne caused by various reasons is excellent. The plant acne-removing composition is good in stability, high in safety, simple in preparation method, wide in raw material source and suitable for large-scale industrial production.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a plant anti-acne composition and a preparation method thereof. Background Art

[0002] As the pace of life in modern society accelerates, environmental pollution, increased stress, and bad living habits have led to increasingly prominent skin problems. Among them, acne (commonly known as "pimples"), as a common skin disease, has become a problem for many people. Acne mainly occurs on the face, chest, and back, and manifests itself in the form of acne, papules, pustules, and other skin lesions, often accompanied by sebum overflow, which not only affects the patient's appearance, but may also have a negative impact on their psychology.

[0003] The causes of acne are complex and varied, mainly including abnormal androgen levels, abnormal keratinization of cells around hair follicles, and excessive proliferation of Propionibacterium acnes. After puberty, androgen levels in the body increase significantly, promoting the development of sebaceous glands and excessive secretion of sebum. Abnormal keratinization of cells around hair follicles causes the hair follicle opening to become smaller, narrower, or even blocked, making it impossible for sebum to be discharged normally, forming substances that clog the pores and cause acne. In addition, excessive proliferation of Propionibacterium acnes is also one of the important mechanisms for the occurrence of acne. It hydrolyzes triglycerides in sebum to produce free fatty acids, which stimulate inflammatory reactions in and around hair follicles.

[0004] At present, there are many treatments for acne on the market, but most products have problems such as large side effects and easy recurrence. Although traditional chemical drugs have certain therapeutic effects, long-term use may cause adverse reactions such as skin sensitivity and dryness. In recent years, acne removal products based on plant extracts have gradually attracted attention. The various active ingredients in plants not only have anti-inflammatory and antibacterial effects, but also can effectively regulate skin oil secretion and improve the skin microenvironment, thereby achieving the effect of acne removal. Plant extracts have been widely used in the fields of medicine and beauty due to their natural and mild characteristics. However, there are many types of plant acne removal products on the market, but most products have a relatively single formula, limited efficacy, and lack a systematic preparation method. Although some products use a variety of plant extracts, due to unreasonable formulas or improper preparation processes, the stability and safety of the products are affected, and it is difficult to achieve the ideal acne removal effect.

[0005] Therefore, the development of a safe, efficient and natural acne-removing product has become an urgent need of the market. In view of the above problems, the present invention proposes a plant acne-removing composition and a preparation method thereof. Salvia miltiorrhiza Bunge is a dicotyledonous plant, a perennial upright herb of the genus Salvia of the family Labiatae. It is bitter and slightly cold in nature, and it is oriented to the heart, pericardium and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, and relieving restlessness and tranquilizing the mind. Pearl grass (Micranthemum micranthemoides (Nutt.) Wettst.exWettst.) is a plant of the genus Micranthemum of the family Matricariaceae. It is a perennial whole plant submerged herb with flower branches standing in water. Aloe (Aloevera (L.) Burm.f.) is a herbaceous plant of the family Aphrodisiac of the order Liliales. Green tea is a shrub or small tree plant of the genus Camellia of the family Theaceae. It has a natural shape and bright green color. It is made from new leaves or buds of tea trees through processes such as fixing, shaping and drying. Gentiana macrophylla Pall. is a plant of the Gentianaceae family and the genus Gentiana. It is bitter and flat in nature, slightly cold, and enters the stomach, liver, and gallbladder meridians. Its roots are used as medicine. Snail mucus refers to the mucus secreted by snails during crawling. The composition uses a variety of plant extracts with acne-removing effects as core ingredients. Through scientific formula design and sophisticated preparation technology, synergistic effects are generated between the ingredients, thereby improving the acne-removing effect of the product. At the same time, the present invention also focuses on the stability and safety of the product to ensure that the product will not cause irritation or damage to the skin during use. This plant anti-acne composition and its preparation method will provide a safe and effective treatment option for acne patients, meeting the market demand for natural acne-removing products. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a plant anti-acne composition and a preparation method thereof in view of the current status of the prior art. The present invention utilizes a variety of plant extracts with anti-acne efficacy as core ingredients, and through scientific formula design and preparation process, a synergistic effect is generated between the ingredients, thereby improving the anti-acne effect of the product. The preparation method is simple, economical, easy to operate, suitable for industrial production, and has broad market application prospects.

[0007] The technical solution adopted by the present invention to solve the above technical problems is:

[0008] A plant anti-acne composition and a preparation method thereof, characterized in that the plant anti-acne composition comprises the following proportions by weight: 6-14 parts of salvia miltiorrhiza, 5-10 parts of pearl grass, 5-8 parts of aloe vera, 8-12 parts of green tea, 8-16 parts of gentiana macrophylla, and 3-8 parts of snail mucus.

[0009] As a preferred embodiment, the plant anti-acne composition comprises the following proportions by weight: 12 parts of salvia miltiorrhiza, 8 parts of pearl grass, 7 parts of aloe vera, 10 parts of green tea, 10 parts of gentiana macrophylla, and 6 parts of snail mucus.

[0010] The preparation method comprises the following steps:

[0011] (1) Take respective weight portions of Danshen, pearl grass, green tea and Gentiana macrophylla, wash and dry them, grind them into a mixture with an ultrafine grinder, mix them evenly with 75% ethanol solution at a mass ratio of 1:10, perform ultrasonic extraction, the extraction temperature is 50°C, the ultrasonic frequency is 30kHz, the power is 100W, the extraction time is 30min, repeat the extraction three times, combine the extracts, filter, and concentrate the filtrate to 1 / 5 of the original volume by a rotary evaporator under reduced pressure at 40°C, put the concentrate into a freeze dryer, and freeze-dry at -50°C for 10h to obtain a mixed extract;

[0012] (2) Take fresh aloe leaves, peel them with a knife, take out the aloe gel, put them into a high-speed grinder at a speed of 3000 r / min, process them for 2 min to obtain aloe gel slurry, let it stand for 30 min, filter it with four layers of gauze, discard the filter residue, and obtain aloe extract;

[0013] (3) adding 1-3 times the weight of the mixture of deionized water to the mixed extract obtained in step (1), and fully dissolving it using a stirring homogenizer;

[0014] (4) adding the aloe vera extract obtained in step (2), stirring evenly, and then adding snail mucus to prepare a plant anti-acne composition.

[0015] As a preferred embodiment, in step (1), the stirring speed of the stirring homogenizer is 100-1000 r / min, the stirring time is 10-30 min, and the stirring temperature is 30-50°C.

[0016] As a preferred embodiment, the plant anti-acne composition can be mixed with cosmetic adjuvants or used alone as a skin external agent; preferably, in the application, the content of the plant anti-acne composition in the cosmetic is 2-20wt%.

[0017] As a preferred embodiment, the above-mentioned cosmetic adjuvants include moisturizers, antioxidants, emulsifiers, cosolvents, preservatives, pH adjusters, skin conditioners, fillers and thickeners.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The botanical anti-acne composition of the present invention uses extracts of natural animals and plants such as salvia miltiorrhiza, pearl grass, aloe, green tea, gentiana macrophylla and snail mucus as active ingredients, does not contain any chemical synthetic additives, avoids the irritation, allergy and toxicity problems that may exist in traditional acne-removing products, is mild and non-irritating to the skin, and has high safety.

[0020] (2) The plant anti-acne composition of the present invention acts on the acne site to play an anti-inflammatory and bactericidal role, which can effectively remove acne, fade acne marks, and inhibit acne regeneration. The combination of salvia miltiorrhiza extract, green tea extract and gentiana macrophylla extract has the effects of antibacterial and anti-inflammatory, preventing acne from further deterioration, and quickly and effectively removing acne. Green tea extract has an antioxidant effect, which can remove free radicals in the skin, reduce the oxidative stress damage caused by acne, and promote the disappearance of inflammation. Salvia miltiorrhiza extract has a strong antibacterial and anti-inflammatory effect, which can inhibit the deterioration of acne and stabilize the skin. Gentiana macrophylla extract contains active ingredients such as gentiopicroside and gentianine, which can further regulate skin quality and reduce the recurrence of acne.

[0021] Snail mucus, aloe vera extract, and pearl grass extract are combined to accelerate the metabolism of skin keratin, fade pigments, eliminate acne marks, improve uneven skin tone caused by acne marks, and promote the balance of water and oil in the skin, prevent the skin from secreting too much oil and clogging the hair follicles again, establish a skin barrier, and inhibit the regeneration of acne. Pearl grass extract can promote the circulation of qi and blood, improve the metabolism of the skin, promote epidermal metabolism, repair skin cells, fade pigments, eliminate acne marks, and establish a skin barrier. Aloe vera extract has a calming and soothing effect on the skin, which can help relieve skin discomfort caused by acne. Snail mucus is rich in collagen, elastin, and other active substances, which help promote the regeneration and repair of skin cells, thereby effectively eliminating acne marks and improving skin texture. The above components work synergistically to achieve the effect of removing acne and eliminating acne marks.

[0022] (3) The plant anti-acne composition of the present invention ensures the stability of the product through careful formulation and optimized process, so that it can be stored for a long time in different environments without affecting the effect. DETAILED DESCRIPTION

[0023] Below in conjunction with embodiment, specific embodiments of the present invention are described in detail, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work belong to the scope of protection of the present invention. The experimental methods in the following embodiments, if not otherwise specified, are conventional methods. The test materials used in the following embodiments, if not otherwise specified, are purchased from conventional biochemical reagent stores.

[0024] "h" represents the time unit "hour"; "℃" represents the temperature "degrees Celsius"; "min" represents the time unit "minute"; "g" represents the weight unit "gram"; "kHz" represents the frequency unit "kilohertz"; "W" represents the power unit "watt"; "r / min" represents the speed unit "revolutions / minute"; "wt%" represents the unit of weight (mass) percentage; "μL" represents the volume unit "microliter"; "ml" represents the volume unit "milliliter"; "mol / L" represents the concentration unit; "CFU / ml" represents colony forming unit.

[0025] Example 1

[0026] A plant anti-acne composition comprises the following proportions by weight: 120 g of salvia miltiorrhiza, 80 g of pearl grass, 70 g of aloe vera, 100 g of green tea, 100 g of gentiana macrophylla, and 60 g of snail mucus.

[0027] The specific steps are as follows:

[0028] (1) Take respective weight portions of Danshen, pearl grass, green tea and Gentiana macrophylla, wash and dry them, grind them into a mixture with an ultrafine grinder, mix them evenly with 75% ethanol solution at a mass ratio of 1:10, perform ultrasonic extraction, the extraction temperature is 50°C, the ultrasonic frequency is 30kHz, the power is 100W, the extraction time is 30min, repeat the extraction three times, combine the extracts, filter, and concentrate the filtrate to 1 / 5 of the original volume by a rotary evaporator under reduced pressure at 40°C, put the concentrate into a freeze dryer, and freeze-dry at -50°C for 10h to obtain a mixed extract;

[0029] (2) Take fresh aloe leaves, peel them with a knife, take out the aloe gel, put them into a high-speed grinder at a speed of 3000 r / min, process them for 2 min to obtain aloe gel slurry, let it stand for 30 min, filter it with four layers of gauze, discard the filter residue, and obtain aloe extract;

[0030] (3) adding deionized water twice the weight of the mixture to the mixed extract obtained in step (1), and dissolving it fully with a stirring homogenizer at a stirring speed of 200 r / min, a stirring time of 30 min, and a stirring temperature of 30° C.;

[0031] (4) adding the aloe vera extract obtained in step (2), stirring evenly, and then adding snail mucus to prepare a plant anti-acne composition.

[0032] Example 2

[0033] A plant anti-acne composition comprises the following proportions by weight: 100 g of salvia miltiorrhiza, 80 g of pearl grass, 70 g of aloe vera, 100 g of green tea, 120 g of gentiana macrophylla, and 60 g of snail mucus.

[0034] The specific steps are the same as those in Example 1.

[0035] Example 3

[0036] A plant anti-acne composition comprises the following proportions by weight: 120 g of salvia miltiorrhiza, 80 g of pearl grass, 70 g of aloe vera, 120 g of green tea, 80 g of gentiana macrophylla, and 60 g of snail mucus.

[0037] The specific steps are the same as those in Example 1.

[0038] Example 4

[0039] A plant anti-acne composition comprises the following proportions by weight: 120 g of salvia miltiorrhiza, 100 g of pearl grass, 50 g of aloe vera, 100 g of green tea, 100 g of gentiana macrophylla, and 60 g of snail mucus.

[0040] The specific steps are the same as those in Example 1.

[0041] Example 5

[0042] A plant anti-acne composition comprises the following proportions by weight: 120 g of salvia miltiorrhiza, 80 g of pearl grass, 50 g of aloe vera, 100 g of green tea, 100 g of gentiana macrophylla, and 80 g of snail mucus.

[0043] The specific steps are the same as those in Example 1.

[0044] Example 6

[0045] A plant anti-acne composition comprises the following proportions by weight: 120 g of salvia miltiorrhiza, 60 g of pearl grass, 70 g of aloe vera, 100 g of green tea, 100 g of gentiana macrophylla, and 80 g of snail mucus.

[0046] The specific steps are the same as those in Example 1.

[0047] Comparative Example 1

[0048] The preparation process is the same as in Example 1, only 530 g of Salvia miltiorrhiza is included, which is washed, dried, crushed, and then ultrasonically extracted three times, concentrated, and freeze-dried to obtain an extract, and 2 times the weight of deionized water is added, and fully stirred to dissolve. Other parameters are the same as in Example 1.

[0049] Comparative Example 2

[0050] The preparation process is the same as in Example 1, except that 530 g of pearl grass is included, and other parameters are the same as in Example 1.

[0051] Comparative Example 3

[0052] The preparation process is the same as in Example 1, only 530 g of aloe vera is included, the aloe vera gel is taken out after peeling, crushed, allowed to stand, and filtered to obtain the aloe vera extract. Other parameters are the same as in Example 1.

[0053] Comparative Example 4

[0054] The preparation process is the same as in Example 1, except that only 530 g of green tea is included and other parameters are the same as in Example 1.

[0055] Comparative Example 5

[0056] The preparation process is the same as in Example 1, except that only 530 g of Gentiana macrophylla is included, and other parameters are the same as in Example 1.

[0057] Comparative Example 6

[0058] The preparation process is the same as in Example 1, only 530 g of snail mucus is included, and other parameters are the same as in Example 1.

[0059] The plant anti-acne compositions of Examples 1 to 6 and the plant anti-acne compositions of Comparative Examples 1 to 6 were respectively added with 0.5% sodium hyaluronate moisturizer, 1% cetearyl glucoside emulsifier, 2% propylene glycol solubilizer, 0.3% potassium sorbate preservative, 0.5% citric acid pH regulator, 0.2% allantoin skin conditioner, 2% magnesium stearate filler and 1% hydroxyethyl cellulose thickener by weight of the composition to prepare cosmetic samples for testing.

[0060] 1. Stability test

[0061] The cosmetic samples prepared from the plant anti-acne compositions of Examples 1 to 6 and the plant anti-acne compositions of Comparative Examples 1 to 6 were placed in thermostats at -15°C, 25°C and 45°C for three months, respectively, and were alternately stored at -15°C, 25°C and 45°C for 3 times, each time for 24 hours, i.e., stored at -15°C for 24 hours, then stored at 25°C for 24 hours, and then placed in a thermostat at 45°C for 24 hours, and the cycle was repeated 3 times in sequence to observe the stability of the samples.

[0062] The evaluation criteria are: o: represents good stability; x: represents the sample is delaminated, precipitated, dehydrated or has a rough appearance. The results are shown in Table 1 below.

[0063] Table 1. High and low temperature stability test results

[0064]

[0065] As can be seen from Table 1, the cosmetic samples prepared from the plant anti-acne compositions of Examples 1 to 6 did not show any stratification, precipitation, water precipitation, or rough appearance under different experimental conditions. The test results show that their stability is better than that of the comparative examples prepared without one or several components.

[0066] 2. Safety Test

[0067] The cosmetic samples prepared from the plant anti-acne compositions of Examples 1 to 6 and the plant anti-acne compositions of Comparative Examples 1 to 6 were subjected to animal skin irritation tests, skin sensitization tests and in vitro cytotoxicity tests according to the cosmetic safety test method, and the results were all safe and non-toxic.

[0068] 3. Anti-Propionibacterium acnes effect test

[0069] In the microbial limit environment, take 200 μL of bacterial suspension and flatten it on a solid culture medium plate, place it in an anaerobic environment at 37 ° C for 24 hours, wash it with 0.03 mol / L phosphate buffer, and make a 104-106 CFU / ml bacterial suspension. Take the plant anti-acne composition of Example 1 to Example 6, and the cosmetic sample of the plant anti-acne composition of Comparative Example 1 to Comparative Example 6, respectively, add 0.1 mL of the above bacterial suspension and evenly coat and mix, add 9 ml of 0.03 mol / l phosphate buffer after 20 minutes, mix thoroughly, and make appropriate dilutions. Take 3 dilutions, take 1 mL respectively and place it in two parallel plates, then add the corresponding culture medium and culture it under anaerobic conditions at 36 ± 1 ° C for 24 hours, and count the colonies. The above test was repeated 3 times, the average was taken, and a blank control group was set.

[0070] The evaluation criteria are: (1) antibacterial rate ≥ 90% is significant effect; (2) antibacterial rate 40-90% is moderate effect; (3) antibacterial rate < 40% is ineffective. The results are shown in the table below.

[0071] Table 2. Anti-Propionibacterium acnes effect test results

[0072] Group Propionibacterium acnes inhibition rate (%) evaluate Example 1 99 Obvious effect Example 2 92 Obvious effect Example 3 93 Obvious effect Example 4 91 Obvious effect Example 5 94 Obvious effect Example 6 93 Obvious effect Comparative Example 1 46 Average effect Comparative Example 2 34 invalid Comparative Example 3 42 Average effect Comparative Example 4 46 Average effect Comparative Example 5 31 invalid Comparative Example 6 43 Average effect

[0073] As can be seen from Table 2, the cosmetic samples prepared from the plant anti-acne compositions of Examples 1 to 6 have obvious antibacterial effects on Propionibacterium acnes, while the cosmetic samples prepared from the plant anti-acne compositions of Comparative Examples 1 to 6 have average or ineffective antibacterial effects on Propionibacterium acnes. The experiment shows that the salvia miltiorrhiza extract, pearl grass extract, aloe extract, green tea extract, gentiana macrophylla extract and snail mucus described in the present invention have a synergistic effect when used in combination in an appropriate ratio, can significantly inhibit the proliferation of Propionibacterium acnes, and the inhibitory effect is better than when any one of them is used alone, and can effectively prevent or improve skin acne symptoms.

[0074] 4. Acne removal effect test

[0075] 240 patients with clinical manifestations of acne, blackheads, cystic, pustular, and papular acne, slight itching or pain, and repeated and difficult to heal were selected and randomly divided into 12 groups, 20 people in each group, and the cosmetic samples of Examples 1 to 6 and Comparative Examples 1 to 6 were used respectively, twice a day, 2 g each time, once on the face after cleaning the face in the morning and evening, and statistics were taken once every 7 days, 15 days, and 30 days to track the acne removal effect.

[0076] The evaluation criteria are: (1) cured: clinical symptoms disappear, acne subsides; (2) effective: clinical symptoms are alleviated, acne partially subsides or disappears less; (3) ineffective: clinical symptoms of acne do not improve. The results are shown in Table 3 below:

[0077] Table 3 Acne removal effect test results

[0078]

[0079] As can be seen from Table 3, the cosmetic samples of Examples 1 to 6 have more significant acne removal effects and faster acne removal speeds than the cosmetic samples of Comparative Examples 1 to 6.

[0080] It should be noted that the above disclosed embodiments only reflect and illustrate the technical solutions of the present invention, and are not intended to limit the protection scope of the present invention. Although the present invention is described in detail with reference to the preferred embodiments, anyone familiar with the technical field should understand that modifications or various changes and equivalent substitutions without departing from the scope of the technical solution of the present invention should all fall within the protection scope of the present invention.

Claims

1. A plant anti-acne composition and a preparation method thereof, characterized in that: The plant anti-acne composition comprises the following proportions by weight: 6-14 parts of salvia miltiorrhiza, 5-10 parts of pearl grass, 5-8 parts of aloe vera, 8-12 parts of green tea, 8-16 parts of gentiana macrophylla, and 3-8 parts of snail mucus.

2. A plant anti-acne composition and a preparation method thereof according to claim 1, characterized in that: The plant anti-acne composition comprises the following proportions by weight: 12 parts of salvia miltiorrhiza, 8 parts of pearl grass, 7 parts of aloe vera, 10 parts of green tea, 10 parts of gentiana macrophylla, and 6 parts of snail mucus.

3. A plant anti-acne composition and a preparation method thereof according to claim 1, characterized in that: The preparation method comprises the following steps: (1) Take respective weight portions of Danshen, pearl grass, green tea and Gentiana macrophylla, wash and dry them, grind them into a mixture with an ultrafine grinder, mix them evenly with 75% ethanol solution at a mass ratio of 1:10, perform ultrasonic extraction, the extraction temperature is 50°C, the ultrasonic frequency is 30kHz, the power is 100W, the extraction time is 30min, repeat the extraction three times, combine the extracts, filter, and concentrate the filtrate to 1 / 5 of the original volume by a rotary evaporator under reduced pressure at 40°C, put the concentrate into a freeze dryer, and freeze-dry at -50°C for 10h to obtain a mixed extract; (2) Take fresh aloe leaves, peel them with a knife, take out the aloe gel, put them into a high-speed grinder at a speed of 3000 r / min, process them for 2 min to obtain aloe gel slurry, let it stand for 30 min, filter it with four layers of gauze, discard the filter residue, and obtain aloe extract; (3) adding 1-3 times the weight of the mixture of deionized water to the mixed extract obtained in step (1), and fully dissolving it using a stirring homogenizer; (4) adding the aloe vera extract obtained in step (2), stirring evenly, and then adding snail mucus to prepare a plant anti-acne composition.

4. A plant anti-acne composition and a preparation method thereof according to claim 3, characterized in that: In step (3), the stirring speed of the stirring homogenizer is 100-1000 r / min, the stirring time is 10-30 min, and the stirring temperature is 30-50° C.

5. A plant anti-acne composition and a preparation method thereof according to claim 1, characterized in that: The plant anti-acne composition can be mixed with cosmetic adjuvants for use, or used alone as a skin external preparation for application; preferably, in the application, the content of the plant anti-acne composition in the cosmetic is 2-20wt%.

6. A plant anti-acne composition and a preparation method thereof according to claim 1, characterized in that: The cosmetic adjuvants in claim 5 include moisturizers, antioxidants, emulsifiers, solubilizers, preservatives, pH adjusters, skin conditioners, fillers and thickeners.

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