Bioreactor for cell tissue engineering

Abstract

The invention discloses a bioreactor for cell tissue engineering, relates to the technical field of bioreactors, and aims to solve the problems that the circulating flow of a liquid material is not ideal and the mixing effect is influenced due to the fact that gas and liquid are not fully guided in a reactor. According to the bioreactor for the cell tissue engineering, the inner blocking cover and the inner guide cylinder are matched, in the process that gas is introduced from the bottom to drive materials to be mixed and stirred, the materials in the inner guide cylinder are gushed upwards, and meanwhile under the cooperation of a through groove and the inner blocking cover, the materials are mixed and stirred. Through the arrangement of the through groove, materials on the outer side of the inner blocking cover can smoothly enter the inner blocking cover through the through groove, the materials can circularly flow, make full contact with gas and be mixed in the driving process when being pneumatically stirred, and meanwhile, the materials can react with oxygen in the introduced gas during cell reaction, so that the reaction efficiency is improved. And the mixing effect of the materials in the reaction tank in the stirring process is improved.

Description

Technical Field

[0001] The invention relates to the technical field of bioreactors, in particular to a bioreactor for cell tissue engineering. Background Art

[0002] At present, bioreactors for cell tissue engineering are key equipment in the field of cell tissue engineering. They provide a suitable environment for cell growth, proliferation and differentiation, and play an important role in the construction and cultivation of biological tissues. Common types of bioreactors for cell tissue engineering include stirred bioreactors and airlift bioreactors. The working principle of the airlift bioreactor is to use the rising effect of gas to achieve the mixing of the reaction liquid. The gas is introduced from the bottom of the reactor to form a gas-liquid mixture, and the liquid is circulated in the reactor to achieve the purpose of mixing and mass transfer.

[0003] However, when the reaction liquid is mixed by the rising effect of the gas, the liquid material circulation flow is not ideal because the gas and liquid are not fully guided in the reactor, which affects the mixing effect. Summary of the invention

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: A bioreactor for cell tissue engineering, comprising:

[0005] A frame body, the frame body having a frame supporting the structural assembly, a connecting fastener being fixedly installed on the outer side of the frame body, and an air pump being fixedly installed on the inner side of the frame body;

[0006] A reaction tank, which provides a place for biological reaction, the reaction tank is fixedly connected to the frame through a connecting fastener, and the reaction tank is connected to the air outlet of the air pump through a pipeline;

[0007] An air stirring mechanism, which is used to stir the material inside the reaction tank by gas flow, and the air stirring mechanism is installed at the bottom of the inner wall of the reaction tank;

[0008] A sealing mechanism, which is used to seal the reaction tank and adjust the internal air pressure, and the sealing mechanism is installed on the top of the reaction tank;

[0009] A flow guiding mechanism, which is used to guide the flow of materials during the stirring process, and the flow guiding mechanism is installed on the top of the air stirring mechanism;

[0010] Wherein, the flow guiding mechanism comprises an inclined block, the inner wall of the inclined block is an inclined surface inclined downward, and an inner guide cylinder is fixedly installed at the bottom of the inner wall of the inclined block, the bottom of the outer side of the inner guide cylinder is evenly provided with through grooves, and an inner blocking cover is fixedly installed at the bottom of the inner wall of the inner guide cylinder, and the inner blocking cover cooperates with the inner guide cylinder, and in the process of mixing and stirring the materials by passing gas at the bottom, the materials inside the inner guide cylinder surge upward, and at the same time, under the cooperation of the through groove and the inner blocking cover, the materials outside the inner blocking cover can smoothly enter the inner blocking cover through the through groove, so that the materials can circulate and fully contact with the gas during pneumatic stirring, and be mixed in the process of being driven, and at the same time, during the cell reaction, the reaction with the oxygen in the introduced gas is improved, so that the mixing effect of the materials in the reaction tank is improved during the stirring process, the top of the inner blocking cover is higher than the through groove, and the inner guide cylinder A fixing ring is fixedly installed on the upper part of the inner wall, and an overflow block is fixedly installed on the top of the fixing ring. The top of the overflow block is flush with the top of the inner guide cylinder, and the outer side of the overflow block is an inclined surface, and the distance between the overflow block and the inner guide cylinder gradually increases from top to bottom. Through the cooperation between the overflow block and the inner guide cylinder, in the process of exporting materials and gases, the flow speed of the materials and gases is improved under the gradually decreasing path width, so that the gas and materials gush out quickly, and after the materials gush out, they can be more evenly diffused to the surroundings at a higher flow rate, thereby improving the mixing effect. At the same time, the stirring wheel is driven to rotate during the flow process, and the materials are mixed with the gas stirring mechanism. A connecting ring is fixedly installed at the center position of the inner wall of the inner guide cylinder, and a sleeve shaft is fixedly installed on the inner wall of the connecting ring. Rotating grooves are symmetrically opened on the outer side of the sleeve shaft, and stirring wheels are rotatably installed at the rotating grooves of the sleeve shaft.

[0011] Preferably, the air stirring mechanism includes a fixed disk and an arc cavity tube, the arc cavity tube is fixedly installed on the bottom of the reaction tank, the outer side of the fixed disk is fixedly connected to the inner wall of the reaction tank, and there is a gap between the fixed disk and the arc cavity tube, the pipeline communication position between the reaction tank and the air pump is located between the fixed disk and the arc cavity tube, and the top of the fixed disk is fixedly connected to the bottom of the oblique block, the inner wall of the fixed disk is installed with a hole tube, and the hole tube is connected with the inner guide tube, the outer side of the hole tube is evenly opened with air guide holes, and the diameter of the air guide holes gradually decreases from the outside to the inside, and the inner wall of the arc cavity tube is fixedly installed with a sliding cavity tube, and the sliding cavity tube The arc cavity tube is evenly installed along the center position of the circle, and a sliding rod is slidably installed on the inner wall of the sliding cavity tube, and a blocking block is fixed at one end of the sliding rod. The outer side of the blocking block is an inclined surface, and the blocking block is slidingly adapted to the air guide hole of the hole tube, and a first spring is fixedly installed between the blocking block and the sliding cavity tube, and an arc cover disk is fixedly installed at the other end of the sliding rod, and the arc cover disk cooperates with the blocking block. After the air pressure reaches the pressure that deforms the first spring, the gap between the blocking block and the air guide hole of the hole tube can be opened to introduce the gas into the hole tube. At the same time, the hole tube cooperates with the inner guide tube to realize the circulation of materials, and the outer side of the arc cover disk is slidingly adapted to the inner wall of the sliding cavity tube.

[0012] Preferably, the sealing mechanism comprises a sealing cover, the inner wall of the sealing cover is detachably mounted on the top of the reaction tank, and an inner plate is fixedly mounted on the inner wall of the sealing cover, a beveled cylinder is fixedly mounted on the inner wall of the inner plate, a perforated disk is fixedly mounted on the top of the outer side of the beveled cylinder, and the outer side of the beveled cylinder is a bevel inclined inwardly from top to bottom, a rubber ring is fixedly mounted on the bottom of the outer side of the sealing cover, and a slip ring is slidably mounted on the outer side of the beveled cylinder, and through the cooperation between the slip ring and the beveled cylinder, when the internal air pressure of the material increases during the pneumatic stirring process, it can only be discharged through the gap between the pressure relief cylinder and the sealing block, and the seal between the sealing cover and the reaction tank, After being pressurized by the slip ring, the pressure is transmitted to the outside through the inclined surface of the inclined surface cylinder, thereby increasing the fitting pressure between the rubber ring and the reaction tank and improving the sealing performance. A pressure relief cylinder is fixedly installed on the top of the inner wall of the inclined surface cylinder, a support plate is fixedly installed on the bottom of the pressure relief cylinder, a sliding column is slidably installed on the inner wall of the support plate, a second spring is fixedly installed on the top of the outer side of the sliding column, and the bottom end of the second spring is fixedly connected to the top of the support plate. A pressure relief groove is opened at the center position of the top of the pressure relief cylinder, and the aperture of the pressure relief groove gradually decreases from top to bottom, and a sealing block is fixedly installed on the top of the sliding column, and the sealing block is slidably adapted to the pressure relief groove of the pressure relief cylinder.

[0013] The present invention provides a bioreactor for cell tissue engineering, which has the following beneficial effects:

[0014] 1. The bioreactor for cell tissue engineering cooperates with the inner baffle cover and the inner guide tube. When gas is introduced at the bottom to drive the material to be mixed and stirred, the material inside the inner guide tube flows upward. At the same time, with the cooperation of the through groove and the inner baffle cover, the material outside the inner baffle cover can smoothly enter the inner baffle cover through the through groove, so that the material can circulate and fully contact with the gas during pneumatic stirring, and be mixed in the driving process. At the same time, when the cells react, they react with the oxygen in the introduced gas, so that the mixing effect of the material in the reaction tank is improved during the stirring process.

[0015] 2. The bioreactor for cell tissue engineering, through the cooperation between the overflow block and the inner guide tube, can increase the flow speed of the material and gas under the gradually decreasing path width during the process of exporting the material and gas, so that the gas and material can flow out quickly. After the material flows out, it can be diffused more evenly to the surroundings at a higher flow rate, thereby improving the mixing effect. At the same time, the stirring wheel is driven to rotate during the flow process, and the material is mixed in cooperation with the gas stirring mechanism.

[0016] 3. The bioreactor for cell tissue engineering cooperates with the arc cover plate and the block. After the air pressure reaches the pressure that causes the first spring to deform, the gap between the block and the air guide hole of the hole tube can be opened to introduce the gas into the hole tube. At the same time, the hole tube cooperates with the inner guide tube to realize the circulation of materials.

[0017] Fourth, the bioreactor for cell tissue engineering, through the cooperation between the slip ring and the bevel cylinder, can only be discharged through the gap between the pressure relief cylinder and the sealing block when the internal air pressure increases during the pneumatic stirring process of the material. After the seal between the cover and the reaction tank is pressurized by the slip ring, the pressure is transmitted to the outside through the bevel of the bevel cylinder, thereby increasing the fitting pressure between the rubber ring and the reaction tank and improving the sealing performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the external structure of a bioreactor for cell tissue engineering of the present invention;

[0019] Figure 2 This is a partial structural anatomical diagram of a bioreactor for cell tissue engineering of the present invention;

[0020] Figure 3 It is a schematic diagram of the structure of the flow guiding mechanism of the present invention;

[0021] Figure 4 This is a structural anatomical diagram of the flow guiding mechanism of the present invention;

[0022] Figure 5 This is a schematic diagram of the structure of the air stirring mechanism of the present invention;

[0023] Figure 6It is a partial structural schematic diagram of the air stirring mechanism of the present invention;

[0024] Figure 7 It is a partial structural side view of the air stirring mechanism of the present invention;

[0025] Figure 8 It is a schematic diagram of the sealing mechanism structure of the present invention;

[0026] Fig. 9 It is a structural anatomical diagram of the sealing mechanism of the present invention.

[0027] In the figure: 1, frame; 2, flow guide mechanism; 3, air stirring mechanism; 4, sealing mechanism; 5, reaction tank; 6, connecting fastener; 7, air pump; 21, oblique stopper; 22, inner guide cylinder; 23, overflow block; 24, inner stop cover; 25, connecting ring; 26, stirring wheel; 27, sleeve shaft; 28, fixed ring; 31, fixed disk; 32, arc cavity tube; 33, hole tube; 34, sliding cavity tube; 35, arc cover disk; 36, first spring; 37, sliding rod; 38, blocking block; 401, cover; 402, inner disk; 403, sealing block; 404, pressure relief cylinder; 405, rubber ring; 406, hole disk; 407, sliding ring; 408, sliding column; 409, second spring; 410, support disk; 411, inclined cylinder. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for specific uses.

[0029] The first embodiment, as Figures 1 to 4 As shown, the present invention provides a technical solution: a bioreactor for cell tissue engineering, comprising:

[0030] A frame 1 having a frame supporting the structural components, a connecting fastener 6 is fixedly installed on the outside of the frame 1, and an air pump 7 is fixedly installed inside the frame 1;

[0031] A reaction tank 5, which provides a place for biological reaction, is fixedly connected to the frame 1 through a fastener 6, and is connected to the air outlet of the air pump 7 through a pipeline;

[0032] An air stirring mechanism 3, which is used to stir the material inside the reaction tank 5 by gas flow, and the air stirring mechanism 3 is installed at the bottom of the inner wall of the reaction tank 5;

[0033] A sealing mechanism 4, which is used to seal the reaction tank 5 and adjust the internal air pressure, and the sealing mechanism 4 is installed on the top of the reaction tank 5;

[0034] A flow guiding mechanism 2, which is used to guide the flow of materials during the stirring process, and the flow guiding mechanism 2 is installed on the top of the air stirring mechanism 3;

[0035] The guide mechanism 2 includes an inclined stopper 21, the inner wall of the inclined stopper 21 is an inclined surface inclined downward, and an inner guide cylinder 22 is fixedly installed at the bottom of the inner wall of the inclined stopper 21, through grooves are evenly opened at the bottom of the outer side of the inner guide cylinder 22, and an inner stop cover 24 is fixedly installed at the bottom of the inner wall of the inner guide cylinder 22, the top of the inner stop cover 24 is higher than the through groove, and a fixing ring 28 is fixedly installed above the inner wall of the inner guide cylinder 22, and an overflow block 23 is fixedly installed on the top of the fixing ring 28. In the process of introducing gas through the gas stirring mechanism 3 to drive the material to surge upward, through the communication between the inner guide cylinder 22 and the hole cylinder 33, the gas drives the material to surge upward along the internal space of the inner guide cylinder 22 under the cooperation between the hole cylinder 33 and the inner guide cylinder 22, and at the same time, in the process of the material and the gas surging upward, the stirring wheel 26 is driven to rotate under the restriction of the sleeve shaft 27 to stir the passing material, so that the material is stirred in the surging process. The top of the overflow block 23 is flush with the top of the inner guide cylinder 22, and the outer side of the overflow block 23 is an inclined surface, and the distance between the overflow block 23 and the inner guide cylinder 22 gradually increases from top to bottom. A connecting ring 25 is fixedly installed at the center position of the inner wall of the inner guide cylinder 22, and a sleeve shaft 27 is fixedly installed on the inner wall of the connecting ring 25. In the process of leading out the inner guide cylinder 22, the overflow block 23 cooperates with the inner guide cylinder 22 to gradually reduce the lead-out aperture, so that the material and gas are quickly led out, and after the lead-out, the material at the bottom of the reaction tank 5 enters the inner guide cylinder 22 again through the through grooves uniformly opened on the outer side of the inner guide cylinder 22 under the guidance of the inclined stopper 21, and in the process of leading in, the airflow is blocked by the inner stopper 24, so that the material on the outer side of the inner guide cylinder 22 can smoothly enter the inner guide cylinder 22. The outer side of the sleeve shaft 27 is symmetrically provided with rotating grooves, and the rotating grooves of the sleeve shaft 27 are rotatably installed with stirring wheels 26.

[0036] The second embodiment is based on the first embodiment. Figures 5 to 7As shown, the gas stirring mechanism 3 includes a fixed plate 31 and an arc cavity tube 32, the arc cavity tube 32 is fixedly installed at the bottom of the reaction tank 5, the outer side of the fixed plate 31 is fixedly connected to the inner wall of the reaction tank 5, and there is a gap between the fixed plate 31 and the arc cavity tube 32, the pipeline connecting the reaction tank 5 and the air pump 7 is located between the fixed plate 31 and the arc cavity tube 32, and the top of the fixed plate 31 is fixedly connected to the bottom of the oblique stopper 21, the inner wall of the fixed plate 31 is installed with a hole tube 33, and the hole tube 33 is connected to the inner guide tube 22, when the air pump 7 introduces gas into the reaction tank 5, the gas First, it enters between the fixed plate 31 and the arc cavity tube 32, and accumulates inside. During the accumulation process, the air pressure gradually increases. After the air pressure gradually increases, the air pressure pushes the arc cover plate 35 to move along the inner wall of the sliding cavity tube 34 into the arc cavity tube 32, so that the arc cover plate 35 drives the sliding rod 37 and the blocking block 38 away from the hole tube, compressing the first spring 36 to deform and compress. The outer side of the hole tube 33 is evenly opened with air guide holes, and the aperture of the air guide holes gradually decreases from the outside to the inside. The inner wall of the arc cavity tube 32 is fixedly installed with the sliding cavity tube 34, and the sliding cavity tube 34 is evenly installed along the center position of the arc cavity tube 32.

[0037] A sliding rod 37 is slidably installed on the inner wall of the sliding cavity tube 34, and a blocking block 38 is fixed at one end of the sliding rod 37. The outer side of the blocking block 38 is an inclined surface, and the blocking block 38 is slidably adapted to the air guide hole of the bore tube 33. A first spring 36 is fixedly installed between the blocking block 38 and the sliding cavity tube 34. In the process of the blocking block 38 moving away from the bore tube 33, a gap is opened between the blocking block 38 and the air guide hole of the bore tube 33, so that the gas enters the bore tube 33 through the gap between the air guide hole and the blocking block 38. After entering the bore tube 33, the gas flows upward, driving the material to surge upward at the same time, and the material in the reaction tank 5 is stirred and mixed, so that the material is fully reacted and contacted. An arc cover plate 35 is fixedly installed on the other end of the sliding rod 37, and the outer side of the arc cover plate 35 is slidably adapted to the inner wall of the sliding cavity tube 34.

[0038] The third embodiment is based on the first and second embodiments. Figures 8 to 9As shown, the sealing mechanism 4 includes a sealing cover 401, the inner wall of the sealing cover 401 is detachably mounted on the top of the reaction tank 5, and an inner plate 402 is fixedly mounted on the inner wall of the sealing cover 401, a beveled cylinder 411 is fixedly mounted on the inner wall of the inner plate 402, a perforated disk 406 is fixedly mounted on the top of the outer side of the beveled cylinder 411, and the outer side of the beveled cylinder 411 is a beveled surface that is inclined inward from top to bottom, a rubber ring 405 is fixedly mounted on the bottom of the outer side of the sealing cover 401, and the sealing cover 401 is fixedly connected to the top of the reaction tank 5 and is connected to the rubber ring 405 through a slip ring 407. The rubber ring 405 cooperates so that after the air pressure inside the reaction tank 5 increases, the slip ring 407 is pushed upward, and the inclined surface on the outside of the inclined cylinder 411 is used to transfer the pressure to the rubber ring 405 through the sealing cover 401 after the bottom of the slip ring 407 is subjected to pressure, so that the rubber ring 405 increases the fitting pressure with the inner wall of the reaction tank 5. The slip ring 407 is slidably installed on the outside of the inclined cylinder 411, and the pressure relief cylinder 404 is fixedly installed on the top of the inner wall of the inclined cylinder 411, and the support plate 410 is fixedly installed on the bottom of the pressure relief cylinder 404.

[0039] A sliding column 408 is slidably installed on the inner wall of the support plate 410, and a second spring 409 is fixedly installed on the top of the outer side of the sliding column 408. The bottom end of the second spring 409 is fixedly connected to the top of the support plate 410. A pressure relief groove is opened at the center position of the top of the pressure relief cylinder 404, and the aperture of the pressure relief groove gradually decreases from top to bottom. A sealing block 403 is fixedly installed on the top of the sliding column 408. After the internal air pressure reaches a certain level, the pressure applied to the bottom of the sealing block 403 arrives, so that the sealing block 403 drives the sliding column 408 to move up together, stretching the second spring 409 to deform it, and at the same time, a gap is opened between the sealing block 403 and the pressure relief cylinder 404 to allow gas to flow out and reduce the internal pressure of the reaction tank 5. The sealing block 403 and the pressure relief groove of the pressure relief cylinder 404 are slidably adapted.

[0040] When in use, the staff mixes the required culture reaction materials with the culture solution and puts them into the reaction tank 5, then installs the sealing mechanism 4 on the top of the reaction tank 5 to seal the reaction tank 5, and then starts the air pump 7 to allow the air pump 7 to introduce gas into the reaction tank 5, and guides the introduced gas through the air stirring mechanism 3 to make the gas flow from bottom to top in the reaction tank 5, driving the material in the reaction tank 5 to flow, and in the process of flow, cooperates with the guide mechanism 2 to guide the material inside the reaction tank 5 to circulate, so that the material is fully stirred and mixed for reaction.

[0041] When the air pump 7 introduces the gas into the reaction tank 5, the gas first enters between the fixed disk 31 and the arc cavity tube 32 and accumulates inside. During the accumulation process, the air pressure gradually increases. After the air pressure gradually increases, the air pressure pushes the arc cover disk 35 to move along the inner wall of the sliding cavity tube 34 into the arc cavity tube 32, so that the arc cover disk 35 drives the sliding rod 37 and the blocking block 38 away from the hole tube, and compresses the first spring 36 to deform and compress. At the same time, in the process of the blocking block 38 moving away from the hole tube 33, a gap is opened between the blocking block 38 and the air guide hole of the hole tube 33, so that the gas enters the inside of the hole tube 33 through the gap between the air guide hole and the blocking block 38. After entering the inside of the hole tube 33, due to the upward flow of the gas, the material is driven to surge upward at the same time, and the material inside the reaction tank 5 is stirred and mixed, so that the material is fully reacted and contacted.

[0042] The gas is introduced through the gas stirring mechanism 3 to drive the material to surge upward. In the process of the gas driving the material to surge upward, the gas drives the material to surge upward along the internal space of the inner guide cylinder 22 under the cooperation between the hole cylinder 33 and the inner guide cylinder 22 through the communication between the inner guide cylinder 22 and the hole cylinder 33. At the same time, in the process of the material and the gas surging upward, the stirring wheel 26 is driven to rotate under the restriction of the sleeve shaft 27 to stir the passing material so that the material is fully mixed in the process of surging. At the same time, in the process of leading out the inner guide cylinder 22, the overflow block 23 cooperates with the inner guide cylinder 22 to gradually reduce the leading aperture, so that the material and the gas are quickly led out. After the leading out, the material at the bottom of the reaction tank 5 enters the inner guide cylinder 22 again through the through grooves uniformly opened on the outside of the inner guide cylinder 22 under the guidance of the oblique stopper 21. In the process of leading in, the airflow is blocked by the inner stopper 24, so that the material on the outside of the inner guide cylinder 22 can smoothly enter the inside of the inner guide cylinder 22.

[0043] In the process of sealing the reaction tank 5 by the sealing mechanism 4, the sealing cover 401 is fixedly connected to the top of the reaction tank 5, and cooperates with the rubber ring 405 through the slip ring 407, so that after the internal air pressure of the reaction tank 5 increases, the slip ring 407 is pushed upward, and the inclined surface on the outer side of the inclined cylinder 411 is used to transmit the pressure to the rubber ring 405 through the sealing cover 401 after the bottom of the slip ring 407 is subjected to pressure, so that the rubber ring 405 increases the fitting pressure with the inner wall of the reaction tank 5. At the same time, after the internal air pressure reaches a certain level, the pressure applied to the bottom of the sealing block 403 arrives, so that the sealing block 403 drives the sliding column 408 to move upward together, stretches the second spring 409 to deform it, and at the same time, a gap is opened between the sealing block 403 and the pressure relief cylinder 404 to allow the gas to flow out and reduce the internal pressure of the reaction tank 5.

[0044] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A bioreactor for cell tissue engineering, characterized in that: include: A frame (1), the frame (1) having a frame supporting a structural component, a connecting fastener (6) being fixedly mounted on the outside of the frame (1), and an air pump (7) being fixedly mounted on the inside of the frame (1); A reaction tank (5), which provides a place for biological reaction, wherein the reaction tank (5) is fixedly connected to the frame (1) via a connecting fastener (6), and the reaction tank (5) is connected to the air outlet of the air pump (7) via a pipeline; An air stirring mechanism (3), the air stirring mechanism (3) is used to stir the material inside the reaction tank (5) by means of gas flow, and the air stirring mechanism (3) is installed at the bottom of the inner wall of the reaction tank (5); A sealing mechanism (4), the sealing mechanism (4) is used to seal the reaction tank (5) and adjust the internal air pressure, and the sealing mechanism (4) is installed on the top of the reaction tank (5); A flow guiding mechanism (2), the flow guiding mechanism (2) is used to guide the flow of materials during the stirring process, and the flow guiding mechanism (2) is installed on the top of the air stirring mechanism (3); The guide mechanism (2) comprises an inclined stopper (21), the inner wall of the inclined stopper (21) is an inclined surface inclined downward, and an inner guide tube (22) is fixedly mounted at the bottom of the inner wall of the inclined stopper (21), through grooves are evenly arranged at the bottom of the outer side of the inner guide tube (22), and an inner stopper (24) is fixedly mounted at the bottom of the inner wall of the inner guide tube (22), the top of the inner stopper (24) is higher than the through grooves, and a fixing ring (28) is fixedly mounted above the inner wall of the inner guide tube (22), and an overflow block (23) is fixedly mounted at the top of the fixing ring (28), the top of the overflow block (23) is flush with the top of the inner guide tube (22), the outer side of the overflow block (23) is an inclined surface, and the distance between the overflow block (23) and the inner guide tube (22) gradually increases from top to bottom.

2. A bioreactor for cell tissue engineering according to claim 1, characterized in that: A connecting ring (25) is fixedly mounted at the center of the inner wall of the inner guide cylinder (22), a sleeve shaft (27) is fixedly mounted on the inner wall of the connecting ring (25), rotating grooves are symmetrically formed on the outer side of the sleeve shaft (27), and stirring wheels (26) are rotatably mounted at the rotating grooves of the sleeve shaft (27).

3. A bioreactor for cell tissue engineering according to claim 1, characterized in that: The air stirring mechanism (3) comprises a fixed plate (31) and an arc cavity tube (32); the arc cavity tube (32) is fixedly mounted on the bottom of the reaction tank (5); the outer side of the fixed plate (31) is fixedly connected to the inner wall of the reaction tank (5); a gap exists between the fixed plate (31) and the arc cavity tube (32); a pipeline connecting the reaction tank (5) and the air pump (7) is located between the fixed plate (31) and the arc cavity tube (32); and the top of the fixed plate (31) is fixedly connected to the bottom of the oblique stopper (21).

4. A bioreactor for cell tissue engineering according to claim 3, characterized in that: A hole tube (33) is installed on the inner wall of the fixed plate (31), and the hole tube (33) is communicated with the inner guide tube (22). Air guide holes are evenly opened on the outer side of the hole tube (33), and the diameter of the air guide holes gradually decreases from the outside to the inside. A sliding cavity tube (34) is fixedly installed on the inner wall of the arc cavity tube (32), and the sliding cavity tube (34) is evenly installed along the center position of the arc cavity tube (32).

5. A bioreactor for cell tissue engineering according to claim 4, characterized in that: A sliding rod (37) is slidably mounted on the inner wall of the sliding cavity tube (34), a blocking block (38) is fixed to one end of the sliding rod (37), the outer side of the blocking block (38) is an inclined surface, and the blocking block (38) is slidably matched with the air guide hole of the bore tube (33).

6. A bioreactor for cell tissue engineering according to claim 5, characterized in that: A first spring (36) is fixedly installed between the blocking block (38) and the sliding cavity tube (34), and an arc cover plate (35) is fixedly installed on the other end of the sliding rod (37), and the outer side of the arc cover plate (35) is slidably adapted to the inner wall of the sliding cavity tube (34).

7. A bioreactor for cell tissue engineering according to claim 1, characterized in that: The sealing mechanism (4) comprises a sealing cover (401), the inner wall of the sealing cover (401) is detachably mounted on the top of the reaction tank (5), an inner plate (402) is fixedly mounted on the inner wall of the sealing cover (401), a beveled cylinder (411) is fixedly mounted on the inner wall of the inner plate (402), a perforated disk (406) is fixedly mounted on the top of the outer side of the beveled cylinder (411), and the outer side of the beveled cylinder (411) is a beveled surface that tilts inward from top to bottom.

8. A bioreactor for cell tissue engineering according to claim 7, characterized in that: A rubber ring (405) is fixedly mounted on the bottom of the outer side of the sealing cover (401), a sliding ring (407) is slidably mounted on the outer side of the inclined cylinder (411), a pressure relief cylinder (404) is fixedly mounted on the top of the inner wall of the inclined cylinder (411), and a support plate (410) is fixedly mounted on the bottom of the pressure relief cylinder (404).

9. A bioreactor for cell tissue engineering according to claim 8, characterized in that: A sliding column (408) is slidably mounted on the inner wall of the support plate (410), a second spring (409) is fixedly mounted on the top of the outer side of the sliding column (408), and the bottom end of the second spring (409) is fixedly connected to the top of the support plate (410).

10. A bioreactor for cell tissue engineering according to claim 9, characterized in that: A pressure relief groove is provided at the center of the top of the pressure relief cylinder (404), and the diameter of the pressure relief groove gradually decreases from top to bottom. A sealing block (403) is fixedly installed on the top of the sliding column (408), and the sealing block (403) is slidably adapted to the pressure relief groove of the pressure relief cylinder (404).

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