Composite probiotics and application thereof in sanitary napkin production

Abstract

The invention relates to the technical field of sanitary napkins, in particular to a compound probiotic and application thereof in sanitary napkin production, the compound probiotic combination comprises lactobacillus rhamnosus LC5 with the preservation number of CGMCC No.24837, lactobacillus reuteri DSM17648 with the preservation number of CGMCC No.25389, streptococcus thermophilus with the preservation number of CGMCC 1.1864, and bacillus licheniformis with the preservation number of CGMCC No.27425, the preservation number of bacillus licheniformis with the preservation number of CGMCC No.27425, the preservation number of bacillus licheniformis with the preservation number of CGMCC No.27425, the preservation number of bacillus licheniformis with the preservation number of CGMCC No.27425 and the preservation number of bacillus licheniformis with the preservation number of According to the invention, the composite probiotic composition is applied to production of the sanitary napkin, and the production method of the sanitary napkin which can effectively inhibit the growth of menstrual blood bacteria and protect the vaginal endodermis is provided.

Description

Technical Field

[0001] The invention relates to the technical field of sanitary napkins, and in particular to a composite probiotic and application thereof in the production of sanitary napkins. Background Art

[0002] Sanitary napkins, also known as nursing napkins, are sanitary products used by women during menstruation. The main materials are cotton, non-woven fabrics, pulp or polymers and polymer composite paper formed by composites of the above materials. The side design is mainly used to prevent side leakage. From the inside to the outside, it is divided into three layers: liquid-permeable surface layer, absorbent core, and liquid-impermeable bottom layer. When using, tear off the middle paper tape first, stick the glue side to the middle of the underwear first, and stick the two "ears" to the narrowest part of the crotch.

[0003] Although the above-mentioned prior art can solve the corresponding technical problems, there are still certain defects: during the menstrual period of girls, the accumulation of menstrual blood or other secretions is very easy to cause bacteria to grow, thereby causing bacterial vaginitis or candidal vaginitis, which account for 39% to 89% of clinical vaginal lesions. According to statistics from the World Health Organization (WHO), 90% of Asian women have experienced inflammatory reactions in their private parts, and even more than 50% of patients have missed the golden treatment period due to no obvious symptoms. Many daily small details are not paid attention to, which will cause recurrent chronic gynecological infections, such as: underwear materials are not breathable, wipe from back to front when going to the toilet, wash underwear and socks together, often wear tight jeans, do not change sanitary napkins frequently during menstruation, irregular work and rest, often stay up late, too much pressure or depressed mood, go to high-risk public places, unsafe sexual behavior, and often use cleaning lotion to clean the vagina, etc. Gynecological infections, or further gynecological vaginitis, will have many adverse effects on normal life. Most sanitary napkins or tampons can only absorb menstrual blood or other secretions, but cannot inhibit the growth of bacteria or protect the vaginal epidermis. Summary of the invention

[0004] The purpose of the present invention is to provide a composite probiotic and its application in the production of sanitary napkins in view of the defects and shortcomings of the prior art.

[0005] The Lactobacillus rhamnosus LC5 (preservation number: CGMCC No. 24837) used in the present invention was purchased from the China General Microorganism Culture Collection Administration Center, the Lactobacillus reuteri DSM17648 (preservation number: CGMCC No. 25389) used in the present invention was purchased from the China General Microorganism Culture Collection Administration Center, the Streptococcus thermophilus (preservation number: CGMCC 1.1864) used in the present invention was purchased from the China General Microorganism Culture Collection Administration Center, and the Bacillus licheniformis (preservation number: CGMCC No. 27425) used in the present invention was purchased from the China General Microorganism Culture Collection Administration Center. The four strains in the present invention were obtained as gifts.

[0006] In order to screen out bacteria that can purify harmful bacteria in menstrual blood on sanitary napkins and maintain the skin, the research team of the applicant used the published bacteria in the list of bacteria that can be added to sanitary napkins (each bacteria is selected from a different source) individually and in combination and found that only the combined use of Lactobacillus rhamnosus LC5 (CGMCC No. 24837), Lactobacillus reuteri DSM17648 (CGMCC No.25389), Streptococcus thermophilus (CGMCC 1.1864) and Bacillus licheniformis (CGMCC No.27425) has the effect of purifying harmful bacteria in menstrual blood and maintaining the skin, while the use of Lactobacillus rhamnosus LC5 (CGMCC No.24837), Lactobacillus reuteri DSM17648 (CGMCC No.25389), Streptococcus thermophilus (CGMCC 1.1864) and Bacillus licheniformis (CGMCC No.27425) alone has the effect of purifying harmful bacteria in menstrual blood and maintaining the skin. reuteri)DSM17648 (CGMCC No. 25389), Streptococcus thermophilus (CGMCC 1.1864) or Bacillus licheniformis (CGMCC No. 27425) had no effect of simultaneously killing harmful bacteria and protecting the vaginal epidermis, and other strains of Lactobacillus rhamnosus, Lactobacillus reuteri, Streptococcus thermophilus or Bacillus licheniformis also had no such effect.

[0007] To achieve the above object, the present invention adopts the following technical solutions: In one aspect, the present invention provides a composite probiotic combination, comprising Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis.

[0008] The Lactobacillus rhamnosus LC5 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 24837 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0009] The Lactobacillus reuteri DSM17648 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 25389 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0010] The Streptococcus thermophilus is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC 1.1864 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0011] The Bacillus licheniformis is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 27425 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0012] On the other hand, the present invention provides the use of the composite probiotic combination in the preparation of sanitary napkins.

[0013] As a further embodiment of the present invention, the present invention provides a production method for applying the composite probiotic combination to the production of sanitary napkins, comprising the following steps: Step 1: Activate Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis respectively; Step 2: Processing and shaping the sanitary napkin absorbent core and performing a sterilization process; Step 3: Covering the lower surface of the absorbent core with non-woven fabric, and then covering the lower surface of the non-woven fabric with a waterproof fabric layer; Step 4: Apply the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis to the outer surface of the absorbent core in sequence; Step 5: Covering the upper surface of the absorbent core with non-woven fabric; Step 6: Apply an adhesive layer on the lower surface of the waterproof cloth layer to obtain a finished sanitary napkin.

[0014] Furthermore, the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are successively applied to the outer surface of the absorbent core, and before that, a water guide strip is arranged on the absorbent core by laser welding.

[0015] Furthermore, step two is specifically as follows: stack the non-woven fabric, fluff pulp and polymer water-absorbing material from bottom to top and then weld them together by laser welding, and send them into an ethylene oxide sterilizer for sterilization at a temperature of 49°C-60°C for 4 hours-6 hours, take them out and let them stand for 1 hour, send them into the ultraviolet sterilizer again for 20 minutes-40 minutes of ultraviolet irradiation, and take them out for use.

[0016] Further, in step 4, when inoculating Lactobacillus rhamnosus LC5, the Lactobacillus rhamnosus LC5 is added in the form of Lactobacillus rhamnosus bacterial liquid, and the concentration of Lactobacillus rhamnosus LC5 in the Lactobacillus rhamnosus bacterial liquid is 1.4*10 4 -1.4*10 6 CFU / ml; When inoculating Lactobacillus reuteri DSM17648, the Lactobacillus reuteri DSM17648 is added in the form of a Lactobacillus reuteri bacterial solution, and the concentration of Lactobacillus reuteri DSM17648 in the Lactobacillus reuteri bacterial solution is 2*10 6 -2*10 7 CFU / ml; When inoculating thermophilic streptococci, the thermophilic streptococci are added in the form of thermophilic streptococci liquid, and the concentration of thermophilic streptococci in the thermophilic streptococci liquid is 3*10 5 -3*10 7 CFU / ml; When inoculating Bacillus licheniformis, the Bacillus licheniformis is added in the form of Bacillus licheniformis bacterial solution, and the concentration of Bacillus licheniformis in the Bacillus licheniformis bacterial solution is 2.5*10 5 -2.5*10 7 CFU / ml.

[0017] Furthermore, the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are successively applied to the outer surface of the absorbent core, and before that, an additive is applied to the outer surface of the absorbent core. The additive includes purified water and any one of essential oils, amino acids, glycerol or collagen, or a combination of at least two of them.

[0018] After adopting the above technical solution, the beneficial effects of the present invention are: Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are all highly safe and harmless bacteria. Their derivatives do not contain any ingredients that are harmful to the human body and are highly safe to use.

[0019] The invention provides a probiotic combination which can inhibit and kill bacteria on menstrual blood of sanitary napkins and protect the vaginal epidermis at the same time.

[0020] The probiotic combination is applied to the production of sanitary napkins to provide a sanitary napkin production method that can effectively inhibit the growth of menstrual bacteria and protect the vaginal epidermis. DETAILED DESCRIPTION

[0021] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict. Embodiment 1:

[0022] A composite probiotic combination comprises Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis.

[0023] The Lactobacillus rhamnosus LC5 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 24837 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0024] The Lactobacillus reuteri DSM17648 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 25389 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0025] The Streptococcus thermophilus is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC 1.1864 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0026] The Bacillus licheniformis is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 27425 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0027] The production method of the composite probiotic combination for sanitary napkin production comprises the following steps: Step 1: Activate Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis respectively; Step 2: Process the absorbent core of the sanitary napkin into shape and perform a sterilization process, specifically: stack the non-woven fabric, fluff pulp and polymer absorbent material from bottom to top and then weld them together by laser welding, and send them into an ethylene oxide sterilizer for sterilization at a temperature of 49°C-60°C for 4 hours-6 hours, take them out and let them stand for 1 hour, and then send them into an ultraviolet sterilizer again for ultraviolet irradiation for 20 minutes-40 minutes, and take them out for use; Step 3: Covering the lower surface of the absorbent core with non-woven fabric, and then covering the lower surface of the non-woven fabric with a waterproof fabric layer; Step 4: Apply the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis to the outer surface of the absorbent core in sequence; Step 5: Covering the upper surface of the absorbent core with non-woven fabric; Step 6: Apply an adhesive layer on the lower surface of the waterproof cloth layer to obtain a finished sanitary napkin.

[0028] Further, when inoculating Lactobacillus rhamnosus LC5, the Lactobacillus rhamnosus LC5 is added in the form of Lactobacillus rhamnosus bacterial liquid, and the concentration of Lactobacillus rhamnosus LC5 in the Lactobacillus rhamnosus bacterial liquid is 1.4*10 4 -1.4*10 6 CFU / ml, for example, the concentration is: 1.4*10 5 CFU / ml; When inoculating Lactobacillus reuteri DSM17648, the Lactobacillus reuteri DSM17648 is added in the form of a Lactobacillus reuteri bacterial solution, and the concentration of Lactobacillus reuteri DSM17648 in the Lactobacillus reuteri bacterial solution is 2*10 6 -2*10 7 CFU / ml, for example, the concentration is: 2*10 7 CFU / ml; When inoculating thermophilic streptococci, the thermophilic streptococci are added in the form of thermophilic streptococci liquid, and the concentration of thermophilic streptococci in the thermophilic streptococci liquid is 3*10 5 -3*10 7 CFU / ml, for example, the concentration is: 3*10 5 CFU / ml; When inoculating Bacillus licheniformis, the Bacillus licheniformis is added in the form of Bacillus licheniformis bacterial solution, and the concentration of Bacillus licheniformis in the Bacillus licheniformis bacterial solution is 2.5*10 5 -2.5*10 7 CFU / ml, for example, the concentration is: 2.5*10 6 CFU / ml.

[0029] The inoculation volume of Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis is 0.2ml-0.5ml / cm 2 , that is, every 1cm 2 The surface of the absorbent core of the sanitary napkin is coated with 0.2ml-0.5ml of Lactobacillus rhamnosus LC5, inoculated with Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis (wherein, the ratio of Lactobacillus rhamnosus LC5, inoculated with Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis in the composite probiotic combination is 1-1.5:1-1.5:1-1.5:0.5). The preparation method of the composite probiotic combination is as follows: after activation, Lactobacillus rhamnosus LC5, inoculated with Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are mixed according to the concentration of 1-1.5:1-1.5:1-1.5:0.5 of the number of bacteria, and the total concentration of the bacterial solution is adjusted to 7.65*10 6 ~8.9*10 7 CFU / ml.

[0030] In order to better guide the menstrual blood into the absorbent core and increase the upper limit of the absorption of menstrual blood, the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are successively coated on the outer surface of the absorbent core, and a water guide strip is previously set on the absorbent core by laser welding.

[0031] In order to enable the strains coated on the absorbent core to reproduce better and maintain a certain number of bacteria, and to make the number of bacteria more stable to extend the shelf life of the finished sanitary napkin, the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are successively coated on the outer surface of the absorbent core, and before that, an additive is coated on the outer surface of the absorbent core. The additive includes purified water, and any one of essential oils, amino acids, glycerol or collagen, or a combination of at least two of them. Embodiment 2:

[0032] The production method of the sanitary napkin in this embodiment is similar to that in the first embodiment, except that in step 4, only Lactobacillus rhamnosus LC5 (CGMCC No. 24837), Lactobacillus reuteri DSM17648 (CGMCC No. 25389) and Bacillus licheniformis (CGMCC No. 27425) are added, and Streptococcus thermophilus is not added. Embodiment three:

[0033] The production method of the sanitary napkin in this embodiment is similar to that in the first embodiment, except that in step 4, only Lactobacillus reuteri DSM17648 (CGMCC No. 25389) and Bacillus licheniformis (CGMCC No. 27425) are added, and Streptococcus thermophilus and Lactobacillus rhamnosus LC5 (CGMCC No. 24837) are not added. Embodiment 4:

[0034] The production method of the sanitary napkin in this embodiment is similar to that in the first embodiment, except that: the Bacillus licheniformis (CGMCC No. 27425) in step 4 is replaced by Bacillus licheniformis (CGMCC No. 1.3378), which is purchased from the China General Microbiological Culture Collection Center. Embodiment five:

[0035] The concentration of Staphylococcus aureus and Escherichia coli in ordinary sanitary napkins (cultured with menstrual blood for 2 hours to 5 hours at 38°C) and sanitary napkins with probiotic combination (cultured with menstrual blood for 2 hours to 5 hours at 38°C) was tested; the specific test method is: take out the absorbent core of the product to be tested, and crush the absorbent core into 40-70 mesh coarse powder, take 0.2 grams of the powder and put it into a culture dish, add 20 times the weight of the culture solution to the obtained powder, and culture it at 38°C for 24 hours to 48 hours under the condition of sealing, observe the density of Staphylococcus aureus and Escherichia coli through a microscope, and repeat each experiment 5 times to take the average value. The results are shown in Table 1.

[0036] Table 1 Concentrations of Staphylococcus aureus, Escherichia coli and Mycobacterium tuberculosis in ordinary sanitary napkins (cultured with menstrual blood for 2 hours to 5 hours at 38°C) and sanitary napkins with added probiotic combination (cultured with menstrual blood for 2 hours to 5 hours at 38°C) in different groups

[0037] As can be seen from Table 1, the concentrations of Staphylococcus and Escherichia coli in the sanitary napkin added with the probiotic combination in Example 1 decreased significantly after culture, while the concentrations of Staphylococcus and Escherichia coli in Example 2 and Example 3 decreased very little and were within the error range, indicating that the use of Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis alone or reducing the use of one of the components cannot achieve the effect of significantly reducing the concentrations of Staphylococcus and Escherichia coli. In Example 4, Bacillus licheniformis (CGMCC No. 27425) was replaced by Bacillus licheniformis (Deposit No.: CGMCC 1.3378), the other probiotic combinations remained unchanged, and the concentration of Staphylococcus aureus and Escherichia coli could not be effectively reduced. Embodiment six:

[0038] In this example, the toxicity test of the composite probiotic combination was carried out, and the steps were as follows: According to the 2010 edition of the Chinese Pharmacopoeia, eight SPF mice (weighing approximately 20-25 g) were selected and injected with 0.5 mL of the newly prepared activated bacterial solution of the composite probiotic combination into the mouse peritoneal cavity. The injection volume was 2.3×10 8 CFU / mouse, and measured the weight changes of each mouse every day, and recorded the physiological changes such as the mental state, behavioral activities, etc. of the mice before and after injection, and observed for a total of one week.

[0039] The results showed that there was no death among the 8 mice, no obvious symptoms of poisoning, no adverse reactions, slight weight gain, and normal behavior. This result proves that the compound probiotic combination is highly safe and belongs to the normal non-toxic strain. Embodiment seven:

[0040] In this example, the cell adhesion ability of the composite probiotic combination was verified, and the steps were as follows: Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis stored at -80°C were taken out, inoculated into a centrifuge tube containing 20 mL of MRS liquid culture medium at a ratio of 2%, and cultured at 38°C until the number of viable bacteria reached 3×10 8 CFU / mL (about 24 hours). Two strains of the prior art (Lactobacillus jensenii ATCC 25258 and Lactobacillus gasseri CGMCC No. 16131) were activated and cultured in the same way. The activated and expanded composite probiotic combination bacterial solution was co-cultured with VK2 / E6E7 human vaginal epithelial cells in a 38°C shaker (speed 100 r / min, rotation radius 2 cm) for 2 hours, and then rinsed to wash away the non-adherent bacteria, and the cells were lysed with Triton X-100 to prepare a suspended bacterial solution, and the fresh bacterial solution was quickly taken for counting, and the number of adhered bacteria was divided by the total number of cells to obtain the average number of bacteria adhered to each cell (the results are shown in Table 2).

[0041] Table 2

[0042] From the results in Table 2, it can be seen that, compared with CGMCC No. 16131 and ATCC 25258 in the prior art, the composite probiotic combination has better adhesion to human vaginal epithelial cells VK2 / E6E7 (the average number of adhered single VK2 / E6E7 cells is about 43), and has stronger adhesion than the other groups. In other words, the composite probiotic combination has strong adhesion to vaginal epithelial cells, which is beneficial for it to play an antibacterial role in the human vaginal environment. From the analysis of the results of Example 5, it can be seen that the composite probiotic combination can produce a strong inhibitory ability for pathogenic bacteria. Therefore, the strong adhesion and strong ability to inhibit pathogenic bacteria make the composite probiotic combination a sanitary napkin antibacterial probiotic combination that can effectively prevent the growth of harmful pathogenic bacteria. When wearing a sanitary napkin, the composite probiotic combination can effectively adhere to the epithelial cells of the vaginal epidermis to continuously inhibit harmful bacteria.

[0043] In summary, the inventors found in the process of research and practice that the combined use of Lactobacillus rhamnosus LC5 (CGMCC No. 24837), Lactobacillus reuteri DSM17648 (CGMCC No. 25389), Streptococcus thermophilus (CGMCC1.1864) and Bacillus licheniformis (CGMCC No. 27425) as a composite probiotic combination added to sanitary napkins has a high inhibitory effect on Staphylococcus and Escherichia coli that cause vaginitis, can effectively reduce the probability of vaginitis when wearing sanitary napkins, and improve the protective performance of the vaginal epidermis by attaching the composite probiotic combination to the epidermal cells on the vagina. Lactobacillus rhamnosus LC5 (CGMCC No. 24837), Lactobacillus reuteri DSM17648 (CGMCC No. 25389), Streptococcus thermophilus (CGMCC1.1864) and Bacillus licheniformis (CGMCC No. 27425) are all highly safe strains with guaranteed safety, and their use in the production of sanitary napkins is very promising. However, the combination of three of Lactobacillus rhamnosus LC5 (CGMCC No. 24837), Lactobacillus reuteri DSM17648 (CGMCC No. 25389), Streptococcus thermophilus (CGMCC 1.1864) and Bacillus licheniformis (CGMCC No. 27425), or the Bacillus licheniformis CGMCC1.1856 listed in Example 4 did not have this effect. It is speculated that the metabolites or secondary metabolites produced by these four strains in the metabolic process just stimulated the expression of certain genes, thereby achieving this effect. The specific principle needs further study.

[0044] The above shows and describes the basic principles and main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents. Anything not described in detail in the present invention is a well-known technology of those skilled in the art.

Claims

1. A composite probiotic combination, characterized in that: The composite probiotic combination comprises Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis.

2. A composite probiotic combination according to claim 1, characterized in that: The Lactobacillus rhamnosus LC5 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 24837 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

3. The composite probiotic combination according to claim 1, characterized in that: The Lactobacillus reuteri DSM17648 is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 25389 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

4. The composite probiotic combination according to claim 1, characterized in that: The Streptococcus thermophilus is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC 1.1864 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

5. The composite probiotic combination according to claim 1, characterized in that: The Bacillus licheniformis is deposited in China General Microbiological Culture Collection Center, with a deposit number of CGMCC No. 27425 and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

6. Use of any one of the composite probiotic combinations of claims 1 to 5 in the preparation of sanitary napkins.

7. The use according to claim 6, characterized in that: The production method of the composite probiotic combination for sanitary napkin production comprises the following steps: Step 1: Activate Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis respectively; Step 2: Processing and shaping the sanitary napkin absorbent core and performing a sterilization process; Step 3: Covering the lower surface of the absorbent core with non-woven fabric, and then covering the lower surface of the non-woven fabric with a waterproof fabric layer; Step 4: Apply the activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis to the outer surface of the absorbent core in sequence; Step 5: Covering the upper surface of the absorbent core with non-woven fabric; Step 6: Apply an adhesive layer on the lower surface of the waterproof fabric layer to obtain a finished sanitary napkin.

8. The use according to claim 7, characterized in that: The activated Lactobacillus rhamnosus LC5, Lactobacillus reuteri DSM17648, Streptococcus thermophilus and Bacillus licheniformis are successively smeared on the outer surface of the absorbent core, and before that, a water guide strip is arranged on the absorbent core by laser welding.

9. An application according to claim 7, characterized in that: Step two is as follows: stack the non-woven fabric, fluff pulp and polymer absorbent material from bottom to top and then weld them together by laser welding, and send them into an ethylene oxide sterilizer for sterilization at a temperature of 49°C-60°C for 4 hours-6 hours. After taking them out, let them stand for 1 hour, and then send them into the ultraviolet sterilizer again for 20 minutes-40 minutes of ultraviolet irradiation, and take them out for use.

10. An application according to claim 7, characterized in that: In step 4, when inoculating Lactobacillus rhamnosus LC5, the Lactobacillus rhamnosus LC5 is added in the form of Lactobacillus rhamnosus bacterial liquid, and the concentration of Lactobacillus rhamnosus LC5 in the Lactobacillus rhamnosus bacterial liquid is 1.4*10 4 -1.4*10 6 CFU / ml; When inoculating Lactobacillus reuteri DSM17648, the Lactobacillus reuteri DSM17648 is added in the form of a Lactobacillus reuteri bacterial solution, and the concentration of Lactobacillus reuteri DSM17648 in the Lactobacillus reuteri bacterial solution is 2*10 6 -2*10 7 CFU / ml; When inoculating thermophilic streptococci, the thermophilic streptococci are added in the form of thermophilic streptococci liquid, and the concentration of thermophilic streptococci in the thermophilic streptococci liquid is 3*10 5 -3*10 7 CFU / ml; When inoculating Bacillus licheniformis, the Bacillus licheniformis is added in the form of a Bacillus licheniformis bacterial solution, and the concentration of Bacillus licheniformis in the Bacillus licheniformis bacterial solution is 2.5*10 5 -2.5*10 7 CFU / ml.

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