Colloidal gold fluorescence analysis device

Abstract

The invention discloses a colloidal gold fluorescence analysis device, and relates to the technical field of metering service, the colloidal gold fluorescence analysis device comprises a bottom plate, the top of the bottom plate is fixedly connected with a motor, the top of the motor is provided with a mixing mechanism, and the mixing mechanism and the motor are arranged to intermittently and automatically control a rotating shaft to perform positive and negative rotation work. Through the arrangement of the annular strip, the beaker placed on the annular strip automatically starts to stop after forward rotation for a period of time and then rotates reversely, and the mixture in the beaker is swung and mixed through continuous forward and reverse rotation, so that the condition that the mixture is polluted when a stirring rod is used for stirring is avoided; the problems that the mixing time of manually shaking the beaker is long, manpower is wasted, and the working efficiency is poor are also avoided, the beaker can incline, then return to be right and then incline every time the beaker rotates forwards and backwards, the mixing speed of a mixture in the beaker can be increased along with the continuous inclining motion state of the beaker, and the efficient mixing efficiency of the beaker is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of metrological services, in particular to a colloidal gold fluorescence analysis device. Background Art

[0002] Colloidal gold is a special sol in which gold nanoparticles are evenly dispersed in a solvent. These gold nanoparticles have unique optical properties and can bind to specific biomolecules under certain conditions, thereby being used for biomarkers and detection. The colloidal gold fluorescence analysis device uses these properties and combines fluorescence analysis technology to perform qualitative and quantitative analysis of samples. A colloidal gold fluorescence analysis device described in a patent application with announcement number CN209055463U relates to the field of measurement service technology, and includes a device box, a dust cover is provided on one side of the upper end of the device box, and both ends of the dust cover are rotatably connected to the device box through rotating bolts, and a first placement slot and a second placement slot are respectively provided on one side of the upper end surface of the device box, a sample dish is placed in the first placement slot, and a high-purity water bottle is placed in the second placement slot, and a plurality of sterile slots are evenly arranged inside the device box, and the sterile slots are respectively located on one side of the first placement slot and the second placement slot; In the analysis work, the reagent and sample need to be fully mixed and reacted before the subsequent steps are carried out. When the reagent and sample are poured into the measuring cup and stirred and mixed, it is easy for the attachment on the stirring rod to cause impurities in the final mixed liquid, affecting the accuracy of subsequent analysis. In addition, the stirring of the stirring rod will also cause a large number of bubbles to be generated in the mixed liquid, so that air quickly enters the mixed liquid, accelerates the oxidation reaction, affects the chemical properties of the mixed liquid, and ultimately affects the accuracy of subsequent analysis. Summary of the invention

[0003] In view of the deficiencies in the prior art, the present invention provides a colloidal gold fluorescence analysis device to achieve the purpose of solving the above-mentioned problems.

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: A colloidal gold fluorescence analysis device comprises a bottom plate, a motor is fixedly connected to the top of the bottom plate, and a mixing mechanism is arranged on the top of the motor; The mixing mechanism comprises: A rotating shaft, the rotating shaft is a circular rod-shaped structure, a connecting sleeve is fixedly connected to the upper part of the outer wall of the rotating shaft, the connecting sleeve, the outer wall of the connecting sleeve is fixedly connected to an arc-shaped connecting plate, and the rotating shaft is used to control the rotation of the connecting sleeve; The hinged plate is a plate-like structure, the inner wall of the hinged plate is rotatably connected to a hinge block, the outer wall of the hinge block is fixedly connected to a counterweight block, the outer wall of the counterweight block is fixedly connected to a connecting strip, one end of the connecting strip is fixedly connected to a fixed plate A, and the hinged plate is used to support the hinge block.

[0005] Preferably, one side of the fixing plate A is fixedly connected to a fixing plate B, one side of the fixing plate B is fixedly connected to a fixing plate C, and the fixing plate C is a square plate-shaped structure.

[0006] Preferably, a U-shaped plate is fixedly connected to one side of the fixed plate C, a ring-shaped strip is fixedly connected to the inner wall of the U-shaped plate, and a notch is provided on the top of the ring-shaped strip for placing the mouth of the measuring cup.

[0007] Preferably, a groove is provided on the top of the arc-shaped connecting plate, and the groove is used to provide the closing plate with a tilting space when the closing plate is tilted, and the counterweight block is used to play the role of a counterweight for the hinge block and keep the connecting strip in a vertical state.

[0008] Preferably, the inner wall of the fixed plate C is slidably connected to a closing plate, the closing plate is a parallelogram plate structure, and is located above the annular strip. One side of the fixed plate C is fixedly connected to an elastic rope, and one end of the elastic rope is fixedly connected to the outer wall of the closing plate.

[0009] Preferably, the outer wall of the rotating shaft is provided with a swing mechanism, and the swing mechanism comprises a fixed sleeve, the inner wall of the fixed sleeve is rotatably connected to the outer wall of the rotating shaft, and a long plate is fixedly connected to the bottom of the fixed sleeve.

[0010] Preferably, a fixing bar is fixedly connected to the bottom end of the long board, the fixing bar is an arc-shaped rod-shaped structure, and the bottom end of the fixing bar is fixedly connected to the top of the bottom plate.

[0011] Preferably, the outer wall of the fixed sleeve is fixedly connected with a magnetic block A, the bottom of the annular strip is fixedly connected with a magnetic block B, the magnetic block B is magnetic, the magnetic block A is magnetic, and the magnetism of the magnetic block A and the magnetic block B on the opposite side is that the same poles repel each other.

[0012] The present invention provides a colloidal gold fluorescence analysis device, which has the following beneficial effects: 1. The present invention provides a mixing mechanism, and the motor intermittently and automatically controls the rotating shaft to rotate forward and reverse, so that the beaker placed on the annular bar will automatically start to stop after a period of forward rotation, and then reverse. The continuous forward and reverse rotation is utilized to realize the shaking and mixing of the mixture in the beaker, thereby avoiding the contamination of the mixture caused by stirring with a stirring rod, and also avoiding the problem of manual shaking of the beaker for a long mixing time and wasting manpower, resulting in poor work efficiency.

[0013] 2. The present invention sets a mixing mechanism. Under the inertia of centrifugation, the bottom of the beaker will extend outward and drive the whole beaker, the annular strip, the U-shaped plate, and the fixed plate C to rotate through the hinge block. When the rotating beaker is mixed, the bottom of the beaker itself will naturally extend outward, and the top of the beaker will approach the rotating shaft, so that the beaker is in a tilted state. Each time the beaker rotates forward and reversely, it will tilt and then return to the normal state and tilt again. With its continuously tilted motion state, the mixing speed of the mixture in the beaker will be accelerated, ensuring the high mixing efficiency of the beaker; 3. The present invention provides a mixing mechanism. When the inner wall of the beaker is rotated in an inclined state, the mixed liquid splashed and attached due to the swinging will be accelerated to the bottom of the beaker when the beaker is subjected to the centrifugal rotation force. The mixture in the beaker is mixed while reducing the liquid hanging on the inner wall of the beaker, so that the mixing ratio of the mixture is not affected, thereby ensuring the stability of the mixing.

[0014] 4. The present invention provides a mixing mechanism. During mixing, the two closing plates on both sides of the beaker will move closer to the beaker during rotation, and the two closing plates will slide to a state of being closed together, thereby completing the process of mixing the mixture in the beaker. The opening above the beaker is automatically and quickly closed as much as possible, reducing contact with the air during the swinging and rotating mixing work, avoiding impurities in the air from contaminating the mixture and affecting the accuracy of subsequent analysis and detection.

[0015] 5. The present invention provides a rocking mechanism, so that when the liquid in the beaker is rotated for a long time, the beaker will rock slightly back and forth during the rotation and mixing. The liquid attached to the beaker during the rocking is quickly thrown to the bottom of the beaker and quickly merged into the mixed liquid by the centrifugal force, thereby reducing the dispersion of the mixed liquid. The rotation speed of the motor to control the rotating shaft can also be adjusted to make the beaker and the annular bar rotate at different inclination angles, so as to achieve different spacings between the magnetic block B and the magnetic block A, thereby generating rocking of different amplitudes to correspond to mixing conditions under different circumstances. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 It is a structural schematic diagram of the mixing mechanism of the present invention; Figure 3 It is a structural schematic diagram of the mixing mechanism of the present invention; Figure 4 It is a schematic diagram of the disassembled structure of the mixing mechanism of the present invention; Figure 5 It is a structural schematic diagram of the mixing mechanism of the present invention; Figure 6 It is a structural schematic diagram of the mixing mechanism of the present invention; Figure 7 For the present invention Figure 2 A schematic diagram of the structure of the enlarged view at A; Figure 8 For the present invention Figure 6 A schematic diagram of the structure of the enlarged view at B; Fig. 9 For the present invention Figure 5 A schematic diagram of the structure of the enlarged view at C; Fig.10 The structure of the mixing mechanism of the present invention is shown in FIG. Figure 1 ; Fig.11 The structure of the mixing mechanism of the present invention is shown in FIG. Figure 2 ; Fig.12 The structure of the mixing mechanism of the present invention is shown in FIG. Figure 3 ; Fig.13 It is a structural schematic diagram of the swing mechanism of the present invention; Fig.14 For the present invention Figure 2 Enlarged view of point D.

[0017] In the figure: 1 bottom plate, 2 motor, 3 mixing mechanism, 301 rotating shaft, 302 connecting sleeve, 303 arc connecting plate, 304 hinged plate, 305 hinged block, 306 counterweight, 307 connecting strip, 308 fixed plate A, 309 fixed plate B, 310 fixed plate C, 311 U-shaped plate, 312 annular strip, 313 groove, 314 closing plate, 315 elastic rope, 4 swing mechanism, 401 fixed sleeve, 402 long plate, 403 fixed strip, 404 magnetic block A, 405 magnetic block B. DETAILED DESCRIPTION

[0018] Example 1: Please refer to Figure 1-4 , the present invention provides a technical solution: a colloidal gold fluorescence analysis device, comprising a bottom plate 1, a motor 2 is fixedly connected to the top of the bottom plate 1, and a mixing mechanism 3 is arranged on the top of the motor 2; The hybrid mechanism 3 comprises: The rotating shaft 301 is a round rod-shaped structure, and a connecting sleeve 302 is fixedly connected to the upper part of the outer wall of the rotating shaft 301; A connecting sleeve 302, an arc-shaped connecting plate 303 is fixedly connected to the outer wall of the connecting sleeve 302, and the rotating shaft 301 is used to control the rotation of the connecting sleeve 302; The hinged plate 304 is a plate-like structure. The inner wall of the hinged plate 304 is rotatably connected to a hinge block 305. The outer wall of the hinge block 305 is fixedly connected to a counterweight block 306. The outer wall of the counterweight block 306 is fixedly connected to a connecting strip 307. One end of the connecting strip 307 is fixedly connected to a fixed plate A308. The hinged plate 304 is used to support the hinge block 305. When in use, the reagent and sample are placed in a beaker and can be mixed by shaking the beaker so that the antigen and antibody can fully contact and specifically bind, thereby improving the accuracy and sensitivity of the analysis; Example 2: Please refer to Figure 1-12 On the basis of the first embodiment, the present invention provides a technical solution: a fixed plate B309 is fixedly connected to one side of the fixed plate A308, and a fixed plate C310 is fixedly connected to one side of the fixed plate B309, and the fixed plate C310 is a square plate structure.

[0019] A U-shaped plate 311 is fixedly connected to one side of the fixed plate C310, and a ring-shaped strip 312 is fixedly connected to the inner wall of the U-shaped plate 311. A notch is provided on the top of the ring-shaped strip 312, and the notch is used to place the mouth of the measuring cup.

[0020] A groove 313 is formed on the top of the arc-shaped connecting plate 303, and the groove 313 is used to provide the closing plate 314 with a tilting space when tilting. The counterweight block 306 is used to counterweight the hinge block 305 and keep the connecting strip 307 in a vertical state.

[0021] The inner wall of the fixed plate C310 is slidably connected with a closing plate 314, which is a parallelogram plate structure and is located above the annular strip 312. An elastic rope 315 is fixedly connected to one side of the fixed plate C310, and one end of the elastic rope 315 is fixedly connected to the outer wall of the closing plate 314. When the reagent and sample are poured into the beaker, the beaker can be placed on the annular strip 312, and the outwardly extending part of the top of the beaker will be placed on the annular strip 312. Then, the motor 2 is started to control the rotation of the shaft 301. When the shaft 301 rotates, the connecting sleeve 302 above is driven to rotate. The rotation of the connecting sleeve 302 controls the arc-shaped connecting plate 303 of the outer wall to rotate synchronously with the hinge plate 304 and the hinge block 305. When the hinge block 305 rotates, the fixed plate C310 and the annular strip 312 are driven to rotate synchronously through the fixed plate A308 and the fixed plate B309. The annular strip 312 also drives the beaker above to rotate around the shaft 3 01 performs circular rotation, and when the annular bar 312 rotates with the beaker in a circular rotation, the mixture in the beaker is automatically mixed due to the shaking caused by the rotation, and the motor 2 intermittently automatically controls the rotating shaft 301 to perform forward and reverse rotation, so that the beaker placed on the annular bar 312 will automatically start to stop after a period of forward rotation, and then reverse, and the mixture in the beaker is shaken and mixed by continuous forward and reverse rotation, avoiding the contamination of the mixture caused by stirring with a stirring rod, and also avoiding the problem of manual shaking of the beaker for a long time and wasting manpower, resulting in poor work efficiency; When the annular bar 312 drives the beaker to rotate, the beaker in the annular bar 312 is subjected to centrifugal force during rotation, so that the bottom of the beaker carries the mixture. Under the inertia of the centrifuge, the bottom of the beaker extends outward and drives the entire beaker as well as the annular bar 312, the U-shaped plate 311, and the fixed plate C310 to rotate through the hinge block 305, so that when the rotating beaker is mixed, the bottom of the beaker itself will naturally extend outward, and the top of the beaker will approach the rotating shaft 301, so that the beaker is in a tilted state. Each time the beaker rotates forward and reverses, the beaker will tilt and then return to the normal state and tilt again. With its continuously tilting motion state, the mixing speed of the mixture in the beaker will be accelerated, ensuring the high mixing efficiency of the beaker. At the same time, the mixed liquid splashed and attached to the inner wall of the rotating beaker in the tilted state due to the swing will be accelerated to the bottom of the beaker under the centrifugal force of the beaker. While mixing the mixture in the beaker, the liquid hanging on the inner wall of the beaker is reduced, the mixing ratio of the mixture is not affected, and the mixing stability is guaranteed; When the beaker is placed on the annular bar 312 for rotating mixing, the closing plate 314 sliding on the inner wall of the fixed plate C310 will also be thrown outwards due to the centrifugal force, and the closing plate 314 slides along the inclined sliding groove on the inner wall of the fixed plate C310, and the closing plate 314 pulls the elastic rope 315 to deform and stretch, so that the two closing plates 314 on both sides of the beaker will move closer to the beaker during rotation, and the two closing plates 314 will slide to a state of being closed together, completing the process of mixing the mixture in the beaker, automatically and quickly closing the opening above the beaker as much as possible, reducing the contact with the air during the swinging and rotating mixing work, and preventing impurities in the air from contaminating the mixture and affecting the accuracy of subsequent analysis and detection; Example 3: Please refer to Figure 1-14 On the basis of the first and second embodiments, the present invention provides a technical solution: a swing mechanism 4 is arranged on the outer wall of the rotating shaft 301, the swing mechanism 4 comprises a fixed sleeve 401, the inner wall of the fixed sleeve 401 is rotatably connected to the outer wall of the rotating shaft 301, and a long plate 402 is fixedly connected to the bottom of the fixed sleeve 401.

[0022] A fixing bar 403 is fixedly connected to the bottom end of the long board 402 . The fixing bar 403 is an arc-shaped rod-shaped structure. The bottom end of the fixing bar 403 is fixedly connected to the top of the bottom board 1 .

[0023] The outer wall of the fixed sleeve 401 is fixedly connected with a magnetic block A404, and the bottom of the annular strip 312 is fixedly connected with a magnetic block B405. The magnetic block B405 is magnetic, and the magnetic block A404 is magnetic. The magnetic properties of the magnetic block A404 and the magnetic block B405 on the opposite side are the same polarity repulsion. As the ring bar 312 rotates with the beaker, the magnetic block B405 at the bottom of the ring bar 312 will be closer to the magnetic block A404 due to its tilt. Since the magnetic block A404 is fixed by the fixing sleeve 401, the long plate 402 and the fixing bar 403 and cannot rotate, the ring bar 312 and the magnetic block B405 are driven to rotate all the time. As the magnetic block B405 rotates continuously, it approaches the magnetic block A404 and is pushed by the magnetic block A404 to slightly swing back to the center through the magnetic repulsion of the same poles between the magnetic blocks A404 and B405. When the magnetic block B405 05 When the magnetic block A404 is turned, the beaker will tilt up due to the centrifugal force. When the liquid in the beaker is rotated for a long time to mix, the beaker will swing back and forth slightly during the rotation and mixing. The liquid attached to the beaker during the swinging will be quickly thrown to the bottom of the beaker and quickly integrated into the mixed liquid by the centrifugal force, thereby reducing the dispersion of the mixed liquid. The rotation speed of the rotating shaft 301 can also be controlled by adjusting the motor 2 to make the beaker and the annular bar 312 have different inclination angles when rotating, so as to achieve different spacings between the magnetic block B405 and the magnetic block A404, thereby generating swings of different amplitudes to correspond to the mixing conditions under different circumstances.

[0024] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A colloidal gold fluorescence analysis device, comprising a bottom plate (1), characterized in that: A motor (2) is fixedly connected to the top of the bottom plate (1), and a mixing mechanism (3) is arranged on the top of the motor (2); The mixing mechanism (3) comprises: A rotating shaft (301), the rotating shaft (301) being a circular rod-shaped structure, a connecting sleeve (302) being fixedly connected to the upper portion of the outer wall of the rotating shaft (301), the connecting sleeve (302), an arc-shaped connecting plate (303) being fixedly connected to the outer wall of the connecting sleeve (302), the rotating shaft (301) being used to control the rotation of the connecting sleeve (302); The hinged plate (304) is a plate-shaped structure, the inner wall of the hinged plate (304) is rotatably connected to a hinge block (305), the outer wall of the hinge block (305) is fixedly connected to a counterweight block (306), the outer wall of the counterweight block (306) is fixedly connected to a connecting strip (307), one end of the connecting strip (307) is fixedly connected to a fixing plate A (308), and the hinged plate (304) is used to support the hinge block (305).

2. A colloidal gold fluorescence analysis device according to claim 1, characterized in that: One side of the fixing plate A (308) is fixedly connected to a fixing plate B (309), and one side of the fixing plate B (309) is fixedly connected to a fixing plate C (310), wherein the fixing plate C (310) is a square plate-shaped structure.

3. A colloidal gold fluorescence analysis device according to claim 2, characterized in that: A U-shaped plate (311) is fixedly connected to one side of the fixed plate C (310), a ring-shaped strip (312) is fixedly connected to the inner wall of the U-shaped plate (311), and a notch is provided at the top of the ring-shaped strip (312), the notch being used to place the mouth of a measuring cup.

4. A colloidal gold fluorescence analysis device according to claim 3, characterized in that: The top of the arc-shaped connecting plate (303) is provided with a groove (313), and the groove (313) is used to provide the closing plate (314) with a tilting space when it tilts. The counterweight block (306) is used to play the role of counterweight for the hinge block (305) and keep the connecting bar (307) in a vertical state.

5. A colloidal gold fluorescence analysis device according to claim 4, characterized in that: The inner wall of the fixed plate C (310) is slidably connected to a closing plate (314); the closing plate (314) is a parallelogram-shaped plate structure and is located above the annular strip (312); one side of the fixed plate C (310) is fixedly connected to an elastic rope (315); one end of the elastic rope (315) is fixedly connected to the outer wall of the closing plate (314).

6. A colloidal gold fluorescence analysis device according to claim 5, characterized in that: The outer wall of the rotating shaft (301) is provided with a swing mechanism (4), the swing mechanism (4) comprising a fixing sleeve (401), the inner wall of the fixing sleeve (401) being rotatably connected to the outer wall of the rotating shaft (301), and the bottom of the fixing sleeve (401) being fixedly connected to a long plate (402).

7. A colloidal gold fluorescence analysis device according to claim 6, characterized in that: The bottom end of the long board (402) is fixedly connected to a fixing bar (403), the fixing bar (403) is an arc-shaped rod-shaped structure, and the bottom end of the fixing bar (403) is fixedly connected to the top of the bottom board (1).

8. A colloidal gold fluorescence analysis device according to claim 7, characterized in that: The outer wall of the fixing sleeve (401) is fixedly connected to a magnetic block A (404), and the bottom of the annular strip (312) is fixedly connected to a magnetic block B (405), the magnetic block B (405) is magnetic, and the magnetic block A (404) is magnetic, and the magnetism of the magnetic block A (404) and the magnetic block B (405) on the opposite side is that the same poles repel each other.

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