Polygonatum cyrtonema greenhouse seedling raising technology based on mechanical peeling and refrigeration germination acceleration

Abstract

The invention discloses a polygonatum cyrtonema greenhouse seedling raising technology based on mechanical peeling and refrigeration germination acceleration, and belongs to the technical field of breeding. According to the method, the polygonatum cyrtonema seeds are mechanically peeled by adopting the hexagonal roller screening machine, and the seed kernels are stored under the condition of manual temperature control and humidity control, so that the force is easy to master, the seed mildew and rot phenomenon is effectively relieved, and seed embryos are intact; the seedling raising method has the advantages of being low in embryo germ dip dyeing rate (6% or below), short in seed dormancy time (5 months), short in seedling outplanting time, high in germination rate (98%), large in seed treatment capacity (50 jin can be treated in one batch), low in operation difficulty, high in growth speed and high in seedling quality, and the success rate of large-scale breeding of polygonatum cyrtonema is increased.

Description

Technical Field

[0001] The invention relates to a greenhouse seedling raising technology of Polygonatum cyrtonema based on mechanical peeling, cold storage and germination, and belongs to the technical field of breeding. Background Art

[0002] Polygonatum cyrtonema Hua is a perennial herb of the genus Polygonatum in the Liliaceae family. Its rhizomes are rich in various active ingredients such as polysaccharides, steroidal saponins, alkaloids, flavonoids, phytosterols and volatile oils, which give it pharmacological effects such as anti-aging, anti-tumor, antibacterial, immunity regulation, blood lipid regulation and memory improvement.

[0003] With the development of society, the medicinal and health value of Polygonatum cyrtonema has been increasingly valued by people. The increase in the demand for Polygonatum cyrtonema has led to the predatory excavation of its wild resources, and artificial cultivation is urgently needed. The traditional breeding method of Polygonatum cyrtonema is basically seed and tuber breeding, but the limited tuber quantity cannot meet the development of the Polygonatum cyrtonema industry, and the seeds have dormancy, which greatly restricts the development of the Polygonatum cyrtonema industry. In traditional production, the commonly used seed germination method of Polygonatum cyrtonema is room temperature sand storage germination. This method has a long dormancy time, and room temperature sand storage is prone to breeding bacteria infection, causing seeds to mildew, reducing the quality of seeds, and the management labor costs of pest control, water and fertilizer management, weeding, etc. in traditional seedling management are high. The research on Polygonatum cyrtonema breeding in the prior art is limited to small-batch breeding, and it is difficult to solve the problems of long seed dormancy time and low seed quality encountered in large-scale breeding. Therefore, it is of great significance to develop a method suitable for large-scale breeding of Polygonatum cyrtonema seeds to shorten the dormancy time of Polygonatum cyrtonema seeds and improve seed quality. Summary of the invention

[0004] This patent proposes "mechanical peeling, low-temperature refrigerated germination and large-scale greenhouse seedling technology". The seeds are peeled by mechanical sand grinding and then refrigerated for germination and seedling cultivation, which effectively shortens the seed dormancy period and improves the seed germination rate and quality. The seedlings are grown in special seedbeds in the greenhouse, which is beneficial to prevent diseases and pests, weeds, and moisture conservation during the seedling stage, reduce the cost of seedling management, and make the production of Polygonatum cyrtonema increasingly large-scale and standardized.

[0005] The invention provides a method for raising seedlings of polygonatum sibiricum seeds. The polygonatum sibiricum seeds are subjected to mechanical sand grinding, then stored at low temperatures, germinated after storage, and then sown in a special seedling bed to obtain polygonatum sibiricum seedlings; the special seedling bed uses coconut bran as a main matrix.

[0006] In one embodiment, the mechanical sand mill is to mix the polygonatum seeds with fine sand and then perform mechanical stirring, and the parameters of the mechanical stirring are: 30-40 revolutions per minute, and run for 5 to 15 minutes.

[0007] In one embodiment, the pore size of the fine sand is within 0.4-0.5 cm, and the water content is 10-15%.

[0008] In one embodiment, the mixing ratio of Polygonatum sibiricum seeds and fine sand is (1:4) to (1:2) v / v.

[0009] In one embodiment, the low temperature storage temperature is 0-5°C, and the storage period is 2 to 5 months.

[0010] In one embodiment, the special seedbed includes a lower matrix and an upper matrix; the lower matrix is ​​composed of: high ridges of original nursery soil, geotextile, 1.5-2.5 cm thick decomposed sterile sheep manure, 2.5-3.5 cm thick coconut bran; the upper matrix is ​​2.5-3.5 cm thick coconut bran.

[0011] In one embodiment, the decomposed sterile sheep manure is base fertilizer.

[0012] In one embodiment, Polygonatum cyrtonema seeds are sown between the upper substrate and the lower substrate.

[0013] In one embodiment, Polygonatum sibiricum seeds are mixed with coconut bran in a volume ratio of (1:1) to (1:3) and then sown.

[0014] In one embodiment, the sowing density of Polygonatum sibiricum seeds is 4.5-5.5 kg / 667 m 2 .

[0015] In one embodiment, the germination is as follows: after the preserved Polygonatum sibiricum seeds and wet coconut bran are mixed in a volume ratio of (1:1) to (1:3), the mixture is placed at 15 to 25° C. and 40 to 50% humidity for at least 20 days.

[0016] Beneficial effects:

[0017] (1) Compared with traditional technology, this technology effectively improves the quality of seed preservation. The hexagonal drum screening machine is used to mechanically peel the seeds of Polygonatum cyrtonema, and the seed kernels are stored under artificial temperature and humidity control conditions. The force is easy to control, which effectively reduces the phenomenon of seed mildew and keeps the embryo intact.

[0018] (2) Effectively break seed dormancy and shorten germination time. Using this technology, the dormancy period of Polygonatum cyrtonema seeds is 5 months, effectively shortening the germination time of the seeds.

[0019] (3) Effectively improve seed quality and reduce pathogen infection rate. Using this technology, the seed germination rate can reach as high as 98%.

[0020] (4) Effectively prevent insects and weeds, maintain fertility, and reduce management costs.

[0021] (5) It effectively reduces the transplanting injury rate and makes it easier to transplant the seedlings. The production of Polygonatum cyrtonema is gradually moving towards industrialization and scale. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a real picture of the mature fruit of Polygonatum cyrtonema;

[0023] Figure 2 This is a real picture of the hexagonal drum screening machine;

[0024] Figure 3 This is a real picture of the seeds without cleaning the pulp;

[0025] Figure 4 This is a real picture of the seed kernel after mechanical sanding and peeling;

[0026] Figure 5 This is a real picture of the emergence of seedlings after mechanical peeling and cold storage;

[0027] Figure 6 This is a real picture of the germination of seeds 5 months after mechanical peeling and cold storage to break dormancy;

[0028] Figure 7 Actual photos of the demonstration planting in the special seedbed;

[0029] Figure 8 Comparison of weed growth in coconut bran seedbeds with and without coconut bran covering; the left picture shows an uncovered coconut bran seedbed; the right picture shows a covered coconut bran seedbed. DETAILED DESCRIPTION

[0030] In the following embodiments, the mechanical treatment of seeds refers to the use of a Rongshi single-phase dual-value capacitor motor (produced by Shanghai Rongshi Electric Machinery Co., Ltd. Wenling Branch), commonly known as a hexagonal drum machine, whose overall dimensions are 1600mm long * 1100mm wide, and the internal dimensions are: length 1200mm * width 90mm * height 1200mm) ( Figure 2 ).

[0031] Example 1

[0032] 1. Fruit collection and processing

[0033] Select the Polygonatum cyrtonema plants that are healthy, have large and abundant fruits, are free of pests and diseases, and have strong resistance to stress as the mother plants for seed collection. In September and October, the seeds are ripe when the fruits are full and the skin changes from green to black. Figure 1 ) to pick and remove impurities, put into foam boxes for thin layer composting treatment, which lasts for 10 days. During this period, the boxes are opened to observe the rotting of the pulp. When the fruit rots and separates from the seeds, put it into the sieve for rubbing, and rinse with clean water until the shell and pulp are completely removed. The washed seeds are bright yellow in color, complete in shape, without brown spots, worm holes, and mold and odor. Figure 3 ).

[0034] 2. Mechanical sand grinding of seeds

[0035] The Polygonatum cyrtonema seed coat was subjected to 9 treatments: the first treatment was a seed-sand volume ratio of 1:2, a mesh aperture of 0.5 cm, a wet sand moisture content of 10-15%, a gear speed of 30 rpm, and a continuous operation time of 5 minutes; the second treatment was a seed-sand volume ratio of 1:2, a mesh aperture of 0.5 cm, a wet sand moisture content of 10-15%, a gear speed of 30 rpm, and a continuous operation time of 10 minutes; the third treatment was a seed-sand volume ratio of 1:2, a mesh aperture of 0.5 cm, a wet sand moisture content of 10-15%, a gear speed of 30 rpm, and a continuous operation time of 15 minutes; the fourth treatment was a seed-sand volume ratio of 1:3, a mesh aperture of 0.5 cm, a wet sand moisture content of 10-15%, a gear speed of 30 rpm, and a continuous operation time of 5 minutes; the fifth treatment was a seed-sand volume ratio of 1:3, a mesh aperture of 0.5 cm, a wet sand moisture content of 10-15%, a gear speed of 30 rpm, and a continuous operation time of 5 minutes. m, wet sand moisture content of 10-15%, gear speed of 30 rpm, and continuous operation time of 10 minutes; the sixth treatment is seed sand volume ratio of 1:3, mesh sieve aperture of 0.5 cm, wet sand moisture content of 10-15%, gear speed of 30 rpm, and continuous operation time of 15 minutes; the seventh treatment is seed sand volume ratio of 1:4, mesh sieve aperture of 0.5 cm, wet sand moisture content of 10-15%, gear speed of 30 rpm, and continuous operation time of 5 minutes; the eighth treatment is seed sand volume ratio of 1:4, mesh sieve aperture of 0.5 cm, wet sand moisture content of 10-15%, gear speed of 30 rpm, and continuous operation time of 10 minutes; the ninth treatment is seed sand volume ratio of 1:4, mesh sieve aperture of 0.5 cm, wet sand moisture content of 10-15%, gear speed of 30 rpm, and continuous operation time of 15 minutes.

[0036] As shown in Table 1, the seed damage rates of the two treatments were quite different. The seed damage rates of treatments 3 and 9 were 49% and 41% respectively, while the seed damage rate of treatment 4 was only 5% ( Figure 4 ). Therefore, the best conditions for mechanical sand grinding of seeds are: seed-sand volume ratio 1:3, wet sand moisture content 10-15%, mesh aperture 0.5cm, gear speed 30 rpm, continuous operation time 5 minutes.

[0037] Table 1 Seed damage rate of mechanical dehulling at different seed-sand volume ratios and operation time

[0038]

[0039]

[0040] like Figure 4As shown, the treated seeds have their seed coats removed, exposing the seed kernels (including embryos and endosperms), which need to be disinfected, and the seed kernels are rinsed with a mesh sieve to remove sand and impurities, and soaked in a 1% potassium permanganate solution for 20 minutes. The seeds are taken out and spread out in a cool and ventilated place for 20 minutes to drain the surface moisture, and the safe moisture content is controlled at 8-10% (the appearance is preferably a slight wrinkling of the seed kernel surface).

[0041] 3. Low temperature storage of seeds

[0042] Drain the surface moisture from the seed kernels, put them in breathable woven bags or mesh bags, and store them in a seed germination thermostat at 0-5℃ for 2 months. Turn them over every 3-5 days. When the surface of the seed kernels shrink, spray water to keep them moisturized. Keep the humidity at 8-15%. After 1 month of storage, the infection rate of germ bacteria is only 1%, and after 2 months, the infection rate of germ bacteria is only 3%, which effectively improves the quality of the seeds.

[0043] 4. Germination at room temperature

[0044] In early February of the following spring, take out the seeds from the constant-control box, soak them in 1% potassium permanganate solution for 20 minutes, and then rinse them with clean water for later use. Prepare a foam box with a size of 60*60cm, add coconut bran and spray water to wet it. The humidity of the coconut bran should be such that it does not leak when held in the hand and it will fall apart when loosened (humidity is about 8-15%). Mix the seeds and coconut bran in a volume ratio of (1:1) to (1:3) and place them in a cool and ventilated place outdoors. Be careful not to build a awning and avoid getting wet in the rain. Pay attention to turning them regularly and keep them warm and moist in time (temperature 24-28℃, humidity 10-15%). The seeds will germinate after 20 days.

[0045] After the seeds of Polygonatum cyrtonema were treated with the method of the present invention, the cumulative germination rate reached 98% ( Figure 6 ).

[0046] 5. Land preparation

[0047] (1) Site selection

[0048] When growing seedlings in a shade shed, you should choose a plot that is easy to irrigate, has flat terrain and is not waterlogged.

[0049] (2) Land preparation time

[0050] It is better to deep plow in winter, and the tillage depth of the soil should be no less than 30 cm. After a winter of freezing, the root rot and underground pests during the seedling raising period next year can be reduced.

[0051] (3) Production of special seedbeds

[0052] After clearing the debris from the nursery, break up the soil and rake it flat, deep plow 20-30cm, and apply 50% carbendazim wettable powder 10g / m 2The length of the seedbed is determined by the sowing amount, generally not more than 10 meters, the bed height is 25-30 cm, the bed width is 1.2 meters, and the width of the walkway ditch between the seedbeds is 30 cm.

[0053] Five days before sowing, the entire seedbed is fully covered with a geotextile with a thickness of not less than 0.4 cm, including the artificial working path, so as not to expose the original soil matrix, and fixed with ground nails. On the set geotextile, a layer of fully decomposed sterile sheep manure with a thickness of about 2 cm is spread as base fertilizer, and then a layer of dry coconut bran with a thickness of about 3 cm is spread, and it is raked flat with a nail rake as the lower matrix of the special seedbed.

[0054] The technical effect of the present invention can also be achieved by adjusting the layer thickness of sterile sheep manure to 1.5-2.5 cm and the layer thickness of dry coconut bran to 2.5-3.5 cm.

[0055] 6. Planting seeds

[0056] (1) Seeding

[0057] In early March, the white Polygonatum sibiricum seeds were pressed at 4.5-5.5kg / 667m 2 , spread with coconut bran on the seedbed, where the mass ratio of seeds to coconut bran is (1:1) to (1:3). Note that before sowing, turn on the greenhouse spray equipment to spray the substrate thoroughly with water. After sowing, cover with 2 cm thick coconut bran light substrate (dry coconut bran) and spray it thoroughly with water.

[0058] The technical effect of the present invention can also be achieved by adjusting the covering thickness of the coconut bran to 2.5-3.5 cm.

[0059] (2) Seedling emergence

[0060] About 15 days after sowing, the seeds of Polygonatum sibiricum gradually sprouted, and one month later, the seedlings were basically all out ( Figure 5 ).

[0061] 7. Seedling management

[0062] (1) Water content

[0063] During the germination period, the seedbed substrate should always be kept moist and the humidity should be maintained at 60%; after all the seedlings have emerged, reduce the amount of watering, do not spray unless the soil is dry, and spray thoroughly if you can.

[0064] (2) Shade

[0065] The seedling greenhouse uses a shade net with a shading rate of 50%-70% for shading, and covers a layer of greenhouse film under the shade net. Figure 7 shown.

[0066] (3) Temperature and humidity

[0067] The temperature in the shade shed should be maintained at around 15°C, and the film should be adjusted according to the temperature of the day. When the ambient temperature reaches above 25°C, water should be sprayed once a day, preferably at 10 am; when the temperature in summer reaches above 30°C, water should be sprayed twice a day, at 9 am and 5 pm respectively. Nurseries with conditions can use intermittent spraying, but there should be no accumulation of water in the seedbed.

[0068] (4) Fertilization

[0069] After the leaves of the seedlings have all come out, apply a thin layer of fully decomposed organic fertilizer. Water the plants once after applying the organic fertilizer to avoid inducing diseases. Apply fertilizer 4-5 times throughout the year.

[0070] (5) Disease and pest control

[0071] Pests: The bed placement method of this invention can effectively avoid underground pests, and only leaf-eating pests need to be paid attention to. Use clean water and fertilizer to prevent the spread of insect eggs; regularly clean up diseased plants to reduce the source of pests and diseases. In addition, physical methods such as artificial, light, electricity, and heat can be used to kill or trap pests, avoiding dependence on chemical drugs.

[0072] Diseases: Strengthen water and fertilizer management, promote healthy growth of plants, improve resistance to stress, and effectively control the occurrence of diseases.

[0073] Leaf spot disease mainly harms leaves. In the early stage of the disease, it starts from the base leaves, and faded spots appear on the leaf surface. Later, the lesions expand to become oval or irregular, about 1 cm in size, pale white in the middle, brown on the edges, and with obvious yellow halo near healthy tissues. The lesions are eye-shaped, so it is also called eye spot disease. When the disease is serious, multiple lesions heal and cause leaf withering, and can gradually spread upward, and finally the leaves of the whole plant wither and fall off. Chemical control: 50% methyl thiophanate wettable powder 1000 times solution, 70% mancozeb wettable powder 600 times solution, 75% chlorothalonil wettable powder 600 times solution can be used for spraying control. When it is dry and rainy, the spraying interval can be appropriately extended. Spray once every 7-10 days, and spray continuously for 3-5 times.

[0074] Rhizome rot disease, the rhizome is light brown circular lesions at the early stage of the disease, and then the diseased part rots, the tissue is dispersed and sunken, the lesions are round or oval with a diameter of 5-10mm, and in severe cases, the lesions are connected into large pieces, affecting the yield and quality of Polygonatum. Prevention and control methods: timely irrigate the roots with medicine at the early stage of the disease, and you can choose 500 times of 50% carbendazim or 800 times of 50% antifungal agent; promptly dig out the roots of seriously diseased plants and thoroughly disinfect them with medicine.

[0075] In summary, the seedling raising method of this patent has the advantages of low germ infection rate (below 6%), short seed dormancy time (5 months), short seedling transplanting time, high germination rate (98%), large seed processing capacity (one batch can process 50 kilograms), low operation difficulty, fast growth rate and high seedling quality. The seedlings can be transplanted at 2.5 years old (sprouting and growing in the spring of the first year, continuing to grow in the second year, and can be transplanted in the spring of the third year), and the fresh weight of the tubers is 2.07g; after 2 months of planting under the forest, the fresh weight of the tubers reaches 4.08g.

[0076] Comparative Example 1 Effects of different treatment methods on the infection rate of germ pathogens

[0077] Specific implementation method Referring to Example 1, the difference is that the method of mechanical peeling and refrigeration is replaced by the traditional room temperature sand storage method and the method disclosed in patent CN109566371A where seeds collide with each other to damage the seed coat. The results show that the traditional room temperature sand storage method and the method where seeds collide with each other to damage the seed coat lead to an increase in the infection rate of germs in the seed embryos. The infection rates of germs in the seed embryos are 17% and 42% respectively after 2 months. The infection rate of germs in the seed embryos of the present invention after mechanical peeling and refrigeration for 2 months is only 3%. As shown in Table 2.

[0078] Table 2 Comparison of germ infection rate of different seed treatments and storage methods

[0079] How to save Germ pathogen infection rate after 1 month (%) Germ pathogen infection rate after 2 months (%) Traditional room temperature sand storage 8 17 Collision with each other damages the seed coat 30 42 The invention is refrigerated after mechanical peeling 1 3

[0080] Comparative Example 2 Effects of different treatments on dormancy

[0081] Specific implementation method Referring to Example 1, the difference is that the traditional room temperature sand storage method and the method of damaging the seed coat by mutual collision of seeds disclosed in patent CN 109566371A are used to replace the mechanical collision peeling method of the present invention. The results show that compared with the present invention, the room temperature sand storage method and the method of damaging the seed coat by mutual collision of seeds extend the seed dormancy period. The mechanical peeling and refrigeration of the present invention can effectively shorten the seed dormancy time, as shown in Table 3.

[0082] Table 3 Comparison of dormancy time of different seed treatments and storage methods

[0083]

[0084] It can be seen from Table 3 that, with the "traditional room temperature sand storage" method, 44% of the seeds have a dormancy period of 5 months, which means they can germinate normally in March of the following year, and 43% of the Polygonatum seeds have a dormancy period of up to 17 months, which means they have to wait until March of the third year to germinate normally; with the "patented" low temperature + normal temperature method of matrix storage, the seeds have a dormancy period of 14 months, which means they have to wait until the third year to germinate normally, and the "mechanical peeling and refrigeration" method The seeds have a dormancy period of 5 months, and they can basically germinate in March of the following year, effectively shortening the dormancy time of Polygonatum cyrtonema seeds.

[0085] Comparative Example 3 Effects of different treatments on seed germination rate

[0086] Specific implementation method Referring to Example 1, the difference is that the traditional room temperature sand storage method is used instead of the mechanical collision peeling method described in the present invention. The results show that the room temperature sand storage method causes a significant decrease in seed germination rate, as shown in Table 4.

[0087] Table 4 Comparison of germination rate in the next year under different seed treatments and storage methods

[0088] How to save Cumulative germination rate in the following year (%) Indoor traditional sand storage 44 Mechanical peeling and refrigeration 98

[0089] Comparative Example 4 Effect of Seedling Bed Matrix on Weed Coverage, Annual Manual Weeding Times, and Pest and Disease Infection Rate

[0090] Specific implementation method Referring to Example 1, the difference is that three methods are used to set the Polygonatum sowing seedbed, namely:

[0091] Setting 1: The seedbed is made of loess soil from bottom to top, and sowing is done in the open air under a forest with a canopy density of 0.2-0.4. Sow the seeds directly into the finely prepared soil, cover with 3 cm thick peat soil, and spray thoroughly with water.

[0092] Setting 2: The seedbed is made of loess soil and 3 cm thick peat soil from bottom to top. Sow in the open air under a forest with a canopy density of 0.5-0.7. Sow the seeds directly into the finely prepared soil, cover it with 3 cm thick peat soil, and spray it with water.

[0093] Setting 3: The structure of the special seedbed seeding matrix from bottom to top is: 25cm high ridge of original nursery soil, covered with 4mm thick geotextile, and then covered with 2cm of decomposed sterile sheep manure and 3cm of coconut bran. Seedlings are raised in a shade shed (shading rate 50%-70%), and seeds are directly sown into the above special matrix, covered with 3cm thick coconut bran, and sprayed with water.

[0094] Setting 4: Direct sowing in the original soil of the nursery without covering the coconut bran seedbed.

[0095] Table 5 Comparison of weed coverage, annual manual weeding times, and pest and disease infection rates in different sowing substrates

[0096]

[0097] As can be seen from Table 5, the invention adopts setting 3-the special seedbed uses geotextile as the base, the sheep manure organic matter ensures the nutrient supply for the growth of seedlings, coconut bran is used as a light matrix to cover the seedlings, which keeps moisture and heat, and the upper matrix is ​​separated from the soil by geotextile, which makes it easy to transplant seedlings and effectively prevent the harm of insect pests and weeds in the soil. When coconut bran is not covered in setting 4, there are too many weeds, which is not conducive to management. Figure 8As shown. Settings 1 and 2-the traditional sowing method has a low germination rate and many weeds, with germination rates of 65% and 73%, and weed coverage during the growth period of up to 68% and 55%. Manual weeding is required 6 times and 4 times a year, and the plant disease and insect pest infection rate is 26% and 14%; while setting 3-the special seedling bed has a germination rate of up to 98%, and no weeding is required, the weed control effect is good, and the plant disease and insect pest infection rate is only 3%, which greatly reduces the management cost. During the seedling management of the traditional seedling bed, pest and disease control, water and fertilizer management, weeding, etc. according to conventional seedling raising methods require a large labor cost and cannot form large-scale production. The special seedling bed for Polygonatum sibiricum invented in this invention can effectively prevent the occurrence of underground pests and weeds, while keeping moisture, draining and ventilating, providing a high-quality environment for the growth of Polygonatum sibiricum seedlings. The decomposed sheep manure ensures the sheep manure required for seedling growth, and the moisture retention and drainage characteristics of coconut bran also fit the growth characteristics of Polygonatum sibiricum. The combination of many aspects makes the production of Polygonatum sibiricum seedlings transform to large-scale, intensive and efficient.

[0098] Although the present invention has been disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for raising seedlings of Polygonatum sibiricum seeds, characterized in that: The polygonatum sibiricum seeds are mechanically sand-ground, then stored at low temperature, germinated after storage, and then sown in a special seedling bed to obtain polygonatum sibiricum seedlings; the special seedling bed uses coconut bran as a main matrix.

2. The method according to claim 1, characterized in that The mechanical sand mill is to mix the polygonatum seeds with fine sand and then perform mechanical stirring, wherein the parameters of the mechanical stirring are: 30-40 revolutions per minute, and the operation time is 5-15 minutes.

3. The method according to claim 2, characterized in that The pore size of the fine sand is within 0.4-0.5 cm, and the water content is 10-15%.

4. The method according to claim 3, characterized in that The mixing ratio of Polygonatum sibiricum seeds and fine sand is (1:4) to (1:2) v / v.

5. The method according to claim 4, characterized in that The low temperature storage temperature is 0-5°C, and the storage period is 2 to 5 months.

6. The method according to claim 5, characterized in that The special seedbed comprises a lower matrix and an upper matrix; the lower matrix is ​​composed of: high ridges of original nursery soil, geotextile, 1.5-2.5cm thick decomposed sterile sheep manure, and 2.5-3.5cm thick coconut bran from bottom to top; the upper matrix is ​​2.5-3.5cm thick coconut bran.

7. The method according to claim 6, characterized in that Polygonatum sibiricum seeds are sown between the upper and lower substrates.

8. The method according to claim 7, characterized in that Mix Polygonatum sibiricum seeds and coconut bran in a volume ratio of (1:1) to (1:3) and then sow.

9. The method according to claim 8, characterized in that The sowing density of Polygonatum sibiricum seeds is 4.5-5.5kg / 667m 2 .

10. The method according to claim 9, characterized in that The germination is as follows: after the preserved polygonatum seeds and wet coconut bran are mixed in a volume ratio of (1:1) to (1:3), the mixture is placed at 15 to 25°C and 40 to 50% humidity for at least 20 days.

Images