Corn tissue culture equipment

Abstract

The invention relates to the technical field of tissue culture, and particularly discloses corn tissue culture equipment which comprises a shell and a seedling raising tray, a containing space is formed in the top side of the shell, a plurality of partition plates are fixed to the bottom side of the containing space, the lower half part of the containing space is divided into a plurality of divided spaces by the partition plates, a second pipe body is arranged on each partition plate in a penetrating mode, and the second pipe bodies are connected with the seedling raising tray. The two ends of the second pipe body are bent downwards, the seedling raising plate is located in the containing space and comprises a plurality of cultivation shells which are arranged in the length direction of the shell, the top sides of the cultivation shells are fixedly connected together, a plurality of cultivation grooves are formed in the top side of each cultivation shell, and a plurality of liquid passing holes are formed in the bottom side of each cultivation groove; according to the corn tissue culture equipment, due to the fact that the corn tissue culture equipment is provided with the opening, cleaning is convenient, the plant tissue culture cost is reduced, plant seedlings do not need to be moved in the tissue culture process, and damage to the plant seedlings is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of tissue culture, in particular to a corn tissue culture device. Background Art

[0002] Plant tissue culture mostly uses agar semi-solid culture medium or solid culture medium. Agar culture medium provides nutrients for explants or plant seedlings and has the advantages of simple operation, efficient reproduction, and long-term preservation of high-quality germplasm.

[0003] Glass bottles are common culture containers in plant tissue culture. They have the advantages of high transparency, reusability, and high temperature resistance. However, the mouth of the glass bottle is narrow and the inner wall of the bottle is deep. It must be repeatedly brushed with a special bottle brush, which increases the difficulty and workload of operation. The growth of plants will continuously consume the nutrients in the agar. Due to the size of the glass bottle, it is generally necessary to replace the agar medium every 3 to 6 weeks, which requires a lot of manpower input, increases the cost of plant tissue culture, and replacing the agar medium is easy to damage the root system of the plant seedlings. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the existing defects and provide a corn tissue culture device with an open setting that is easy to clean, reduces the cost of plant tissue culture, does not need to move plant seedlings during tissue culture, and reduces damage to plant seedlings.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a corn tissue culture device, comprising a shell and a seedling tray, the top side of the shell is provided with a accommodating space, the bottom side of the accommodating space is fixed with a plurality of partitions along the length direction of the shell, and the partitions divide the lower half of the accommodating space into a plurality of divided spaces, each partition is penetrated by a second tube body, both ends of the second tube body are bent downward.

[0006] The seedling raising tray is located in the accommodating space, and comprises a plurality of raising shells which are arranged along the length direction of the shells and are fixedly connected together at the top sides, a plurality of raising grooves are provided on the top side of each raising shell, a plurality of liquid holes are provided on the bottom side of each raising groove, a raising shell is inserted in each divided space, and an opening and closing cover is installed on the top side of the shells.

[0007] Among them, culture solution is added to at least one divided space, explants are placed in the cultivation tank, and then the cover body completely covers the accommodating space, and one end of the shell in the length direction is moved upward so that the angle between the shell and the horizontal plane reaches a set angle. At this time, the liquid level of the culture solution in the divided space is higher than the second tube body with a lower height in the divided space.

[0008] The shell is periodically reciprocated around the width direction of the shell at a set angle, so that the culture liquid moves reciprocally along the length direction of the shell.

[0009] As a preferred technical solution of the present invention, a water absorbing block covering the liquid holes is placed at the bottom of each cultivation tank.

[0010] As a preferred technical solution of the present invention, the edge of the seedling tray and the inner wall of the accommodating space are slidably sealed and fitted, the seedling tray closes each divided space, exhaust holes are provided at both ends of the length direction of the seedling tray, and air purification components are installed on the two exhaust holes.

[0011] The air purification component includes a first tube body, a mounting hole is opened on the shell, a second air filter device is installed on the outer side of the mounting hole, the inner side of the mounting hole is connected to one end of the first tube body, and the other end of the first tube body is connected to the top side of the exhaust hole.

[0012] As a preferred technical solution of the present invention, at least one second air filter device is installed with a shielding cloth for adjusting the air flow resistance in the second air filter device.

[0013] As a preferred technical solution of the present invention, a ventilation hole is opened on the cover body, and a first air filter device is installed on the outer side of the ventilation hole.

[0014] As a preferred technical solution of the present invention, two second tube bodies are installed on the partition, one end of the second tube body is close to the bottom of the partition, and the other end of the second tube body has an extension tube extending away from the bottom of the partition, and the extension tubes of the two second tube bodies are located on both sides of the partition.

[0015] As a preferred technical solution of the present invention, a card slot is opened on the top side of the partition, a card block is detachably installed in the card slot, and both ends of the second tube body pass through the card block and extend outside the card block.

[0016] As a preferred technical solution of the present invention, it also includes a base located below the shell, the top side of the base is rotatably connected to the bottom side of the connecting frame, the connecting frame is fixed to the bottom side of the shell, and a driving mechanism for driving the shell to rotate around the width direction of the shell is installed on the base.

[0017] As a preferred technical solution of the present invention, the cover is made of transparent glass or transparent plastic, and the shell is made of transparent glass or transparent plastic.

[0018] As a preferred technical solution of the present invention, the angle between the shell and the horizontal plane is set to 5°-40°.

[0019] Compared with the prior art, the present invention has the following beneficial effects: 1. The corn tissue culture equipment of the present invention is characterized in that the shell periodically reciprocates around the width direction of the shell at a set angle, so that the culture solution reciprocates along the length direction of the shell. After the culture solution stays in one of the divided spaces, it flows to the adjacent and lower divided space. The culture solution flows through each divided space in turn, and the culture solution flows into the cultivation tank through the liquid hole to achieve contact between the culture solution and the roots of the explants or plant seedlings, so that the roots of the explants or plant seedlings are intermittently immersed in the culture solution: on the one hand, the culture solution is used to provide nutrients for the explants or plant seedlings, which reduces labor input, reduces the cost of plant tissue culture, and the tissue culture process There is no need to move the plant seedlings, which reduces the damage to the plant seedlings, and the culture solution is used to soak the explants or roots of the plant seedlings at different positions in different time periods, so the device consumes less culture solution; on the other hand, the culture solution in the shell only fills part of the divided space, and the area of ​​the culture solution surface is small, which reduces the evaporation of water in the culture solution and reduces the vitrification of the leaves of the cultured plant seedlings; on another hand, during the periodic reciprocating rotation of the shell around the width direction of the shell, the culture solution reciprocating along the length direction of the shell realizes the exchange of air inside and outside the shell, so that the harmful gas in the shell is reduced, and the normal respiration of the plant seedlings in tissue culture is guaranteed.

[0020] 2. The corn tissue culture equipment of the example of the present invention adjusts the air flow resistance in the second air filter device by adding or removing a shielding cloth on the second air filter device, thereby adjusting the contact time between the explant or the root of the plant seedling and the culture solution, ensuring that the explant or the root of the plant seedling is in full contact with the culture solution, and improving the reliability of the corn tissue culture equipment.

[0021] 3. In the corn tissue culture equipment of the present invention, a water-absorbing block covering the liquid hole is placed at the bottom of each culture tank. The water-absorbing block absorbs the culture solution to increase the contact time between the explant or the root of the plant seedling and the culture solution.

[0022] 4. In the corn tissue culture equipment of the present invention, the extension tubes of the two second tube bodies are located on both sides of the partition to ensure the siphon effect of the second tube body on the culture solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the main structure of the present invention; Figure 2 It is a schematic diagram of the explosion structure of the present invention; Figure 3 It is a schematic diagram of a partial cross-sectional structure of a housing of the present invention; Figure 4 It is a schematic diagram of the top view of the structure of the seedling tray of the present invention; Figure 5 This is a schematic diagram of the structure of the seedling tray of the present invention when viewed from above; Figure 6It is a schematic diagram of the structure of the housing and the seedling tray of the present invention in an inclined state; Figure 7 It is a schematic diagram of the top view of the shell structure of the present invention.

[0024] In the figure: 1 cover body, 2 first air filter device, 3 second air filter device, 4 shell, 5 drive mechanism, 6 base, 7 seedling tray, 8 first tube body, 9 second tube body, 10 partition, 11 water absorption block, 12 exhaust hole, 13 liquid hole. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0026] Embodiment 1: See also Figure 1-Figure 7 The present embodiment discloses a corn tissue culture device, including a shell 4 and a seedling tray 7. A accommodating space is opened on the top side of the shell 4. A plurality of partitions 10 are fixed on the bottom side of the accommodating space along the length direction of the shell 4, and the partitions 10 divide the lower half of the accommodating space into a plurality of divided spaces. A second tube body 9 is passed through each partition 10, and both ends of the second tube body 9 are bent downward.

[0027] The seedling tray 7 is located in the accommodating space, and the seedling tray 7 includes a plurality of cultivation shells arranged along the length direction of the shell 4 and fixedly connected together at the top side. A plurality of cultivation grooves are provided on the top side of each cultivation shell, and a plurality of liquid holes 13 connecting the divided spaces are provided on the bottom side of each cultivation groove. A cultivation shell is inserted in each divided space, and an openable cover body 1 is installed on the top side of the shell 4.

[0028] The working process and principle of this embodiment are: The operator removes the cover body 1 and the seedling tray 7 from the shell 4, and the operator uses an external sterilization device to sterilize the cover body 1, the seedling tray 7 and the shell 4. The operator adds culture solution into at least one divided space on the sterile operating table, and then the operator puts the seedling tray 7 into the accommodating space of the shell 4. The operator puts explants into each culture groove, and the operator makes the cover body 1 completely cover the accommodating space.

[0029] The operator moves one end of the shell 4 in the length direction upward so that the angle between the shell 4 and the horizontal plane reaches a set angle. At this time, the liquid level of the culture fluid in the dividing space is higher than the second tube body 9 with a lower height in the dividing space. The culture fluid in the dividing space flows into the adjacent and lower dividing space of the dividing space through the second tube body 9, and a siphon phenomenon occurs to the culture fluid at the second tube body 9.

[0030] The operator causes the shell 4 to periodically reciprocate at a set angle around the width direction of the shell 4, so that the culture solution moves back and forth along the length direction of the shell 4. After the culture solution stays in one of the divided spaces, it flows to the adjacent and lower divided space. The culture solution flows through each divided space in turn, and the culture solution flows into the cultivation tank through the liquid hole 13, thereby achieving contact between the culture solution and the roots of the explant or the plant seedling, so that the roots of the explant or the plant seedling are intermittently immersed in the culture solution.

[0031] The corn tissue culture equipment is easy to clean, uses culture solution to provide nutrients for explants or plant seedlings, reduces labor input, and reduces the cost of plant tissue culture. During the tissue culture process, there is no need to move the plant seedlings, which reduces damage to the plant seedlings. The culture solution is used to soak the roots of explants or plant seedlings at different positions in different time periods, and the equipment consumes less culture solution.

[0032] Furthermore, the culture solution in the shell 4 only fills part of the divided space, and the area of ​​the culture solution surface is small, which reduces the evaporation of water in the culture solution and reduces the vitrification of the leaves of the cultivated plant seedlings.

[0033] Furthermore, if Figure 6 As shown, a switch valve connected to the accommodating space is installed on the shell 4, and the operator can replace the culture solution in the accommodating space through the switch valve. The culture solution of this device is easy to replace.

[0034] Furthermore, the included angle between the housing 4 and the horizontal plane is set to be 5°-40°.

[0035] Furthermore, by replacing the cover body 1 of different heights, the device can be adapted to the height of corn seedlings.

[0036] Furthermore, the components of the culture solution include water, inorganic salts, carbohydrates, nitrogen-containing substances, and plant hormones.

[0037] Embodiment 2: like Figure 4 As shown, this embodiment discloses a corn tissue culture device, and its structure is substantially the same as that of the first embodiment, except that, at the bottom of each culture tank of this embodiment, a water-absorbing block 11 is placed to cover the liquid hole 13, and the water-absorbing block 11 is a sponge or a cloth strip.

[0038] The working process and principle of this embodiment are: The water absorbing block 11 absorbs the culture solution, thereby increasing the contact time between the explant or the root of the plant seedling and the culture solution.

[0039] Embodiment three: like Figure 2 , Figure 4 , Figure 6 As shown, the present embodiment discloses a corn tissue culture device, the structure of which is substantially the same as that of the second embodiment, except that the edge of the seedling tray 7 of the present embodiment and the inner wall of the accommodating space are slidingly sealed and fitted, the seedling tray 7 closes each divided space, exhaust holes 12 are provided at both ends of the length direction of the seedling tray 7, and air purification components are installed on the two exhaust holes 12.

[0040] The air purification component includes a first tube body 8, a mounting hole is opened on the shell 4, a second air filter device 3 is installed on the outer side of the mounting hole, the inner side of the mounting hole is connected to one end of the first tube body 8, and the other end of the first tube body 8 is connected to the top side of the exhaust hole 12.

[0041] The working process and principle of this embodiment are: The air or culture solution in two adjacent divided spaces can flow through the second tube body 9 .

[0042] The operator causes the shell 4 to periodically reciprocate at a set angle around the width direction of the shell 4, so that the culture fluid reciprocates along the length direction of the shell 4. The culture fluid divides the division space into two non-connected groups, and the two groups of division spaces are a high-positioned division space group and a low-positioned division space group. After the culture fluid stays in one of the division spaces, it flows to the adjacent and low-positioned division space.

[0043] The volume of the high-positioned divided space group becomes larger, and the water absorption block 11 prevents the air above the seedling tray 7 from entering the high-positioned divided space group, the air pressure in the high-positioned divided space group decreases, and the high-positioned divided space group inhales air through the air purification component.

[0044] The volume of the low-positioned divided space group becomes smaller, so that the air pressure in the low-positioned divided space group increases, and the air in the low-positioned divided space group pushes the water absorption block 11 to enter the space between the seedling tray 7 and the cover body 1, and the air between the seedling tray 7 and the cover body 1 flows out through the gap between the seedling tray 7 and the cover body 1, and the air in the low-positioned divided space group flows to the outside of the shell 4 through the air purification component.

[0045] The corn tissue culture equipment realizes the exchange of air inside and outside the shell 4, reduces harmful gases in the shell 4, and ensures the normal respiration of the plant seedlings in tissue culture.

[0046] Furthermore, the second air filter device 3 is a common air filter device in the prior art, and the second air filter device 3 includes a tube body and a 0.22 micron or 0.45 micron filter membrane in the tube body.

[0047] Embodiment 4: like Figure 6 As shown, this embodiment discloses a corn tissue culture device, the structure of which is substantially the same as that of the third embodiment, except that a shielding cloth for adjusting the air flow resistance in the second air filter device 3 is installed on at least one second air filter device 3 .

[0048] The working process and principle of this embodiment are: The operator adjusts the air flow resistance in the second air filter device 3 by adding or removing a shielding cloth on the second air filter device 3, thereby adjusting the speed at which the culture solution moves along the length direction of the shell 4, thereby adjusting the contact time between the explant or the root of the plant seedling and the culture solution, ensuring that the explant or the root of the plant seedling is in full contact with the culture solution, and improving the reliability of the corn tissue culture equipment.

[0049] Embodiment five: like Figure 1 , Figure 2 As shown, this embodiment discloses a corn tissue culture device, and its structure is substantially the same as that of the third or fourth embodiment, except that a ventilation hole is provided on the cover body 1 of this embodiment, and a first air filter device 2 is installed on the outer side of the ventilation hole.

[0050] The working process and principle of this embodiment are: During the movement of the culture solution along the length direction of the shell 4, the volume of the lower divided space group becomes smaller, so that the air pressure in the lower divided space group increases, and the air in the lower divided space group pushes the water absorption block 11 to enter the space between the seedling tray 7 and the cover body 1, and the air between the seedling tray 7 and the cover body 1 flows to the outside of the shell 4 through the first air filter device 2, and the air in the lower divided space group flows to the outside of the shell 4 through the air purification component.

[0051] The first air filter device 2 can seal the gap between the seedling tray 7 and the cover body 1 to prevent the air outside the shell 4 from entering the shell 4 without being filtered.

[0052] Furthermore, the first air filter device 2 is a common air filter device in the prior art, and the first air filter device 2 includes a tube body and a 0.22 micron or 0.45 micron filter membrane in the tube body.

[0053] Embodiment six: like Figure 2 , Figure 3 , Figure 6As shown, this embodiment discloses a corn tissue culture device, and its structure is roughly the same as that of the first embodiment, except that, in this embodiment, two second tube bodies 9 are installed on the partition 10, one end of the second tube body 9 is close to the bottom of the partition 10, and the other end of the second tube body 9 is extended with an extension tube in the direction away from the bottom of the partition 10, and the extension tubes of the two second tube bodies 9 are located on both sides of the partition 10 to ensure the siphon effect of the second tube body 9 on the culture fluid.

[0054] Embodiment seven: like Figure 2 As shown, this embodiment discloses a corn tissue culture device, and its structure is roughly the same as that of the first embodiment, except that a card slot is provided on the top side of the partition 10 of this embodiment, and a card block is detachably installed in the card slot, and both ends of the second tube body 9 pass through the card block and extend outside the card block, so that the replacement and maintenance of the second tube body 9 are convenient.

[0055] Embodiment eight: like Figure 1 , Figure 2 As shown, this embodiment discloses a corn tissue culture device, and its structure is roughly the same as that of the first embodiment, except that this embodiment also includes a base 6 located below the shell 4, and the top side of the base 6 is rotatably connected to the bottom side of the connecting frame, the connecting frame is fixed to the bottom side of the shell 4, and a driving mechanism 5 for driving the shell 4 to rotate around the width direction of the shell 4 is installed on the base 6.

[0056] The driving mechanism 5 is a jack or an electric push rod.

[0057] The driving mechanism 5 used in the present invention is a common device in the prior art, and its working method and structure are well-known technologies, which will not be described in detail here.

[0058] The working process and principle of this embodiment are: The driving mechanism 5 drives the housing 4 to rotate around the width direction of the housing 4 .

[0059] Embodiment nine: like Figure 2 As shown, this embodiment discloses a corn tissue culture device, and its structure is substantially the same as that of the first embodiment, except that the cover 1 of this embodiment is made of transparent glass or transparent plastic, and the shell 4 is made of transparent glass or transparent plastic.

[0060] The working process and principle of this embodiment are: The operator can observe the growth of the explant or the plant seedling through the cover body 1 , and the operator can observe the position and volume of the culture solution through the shell body 4 .

[0061] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A corn tissue culture device, characterized in that: It comprises a shell (4) and a seedling tray (7), wherein a accommodating space is provided on the top side of the shell (4), a plurality of partitions (10) are fixed on the bottom side of the accommodating space along the length direction of the shell (4), and the partitions (10) divide the lower half of the accommodating space into a plurality of divided spaces, and a second tube (9) is passed through each partition (10), and both ends of the second tube (9) are bent downwards; The seedling tray (7) is located in the accommodating space, and the seedling tray (7) comprises a plurality of cultivation shells arranged along the length direction of the shell (4) and fixedly connected together at the top side, each cultivation shell having a plurality of cultivation grooves on the top side, each cultivation groove having a plurality of liquid holes (13) on the bottom side, a cultivation shell inserted in each divided space, and an openable cover (1) installed on the top side of the shell (4); Wherein, a culture solution is added into at least one divided space, an explant is placed into the culture tank, and then the cover body (1) completely covers the accommodating space, and one end of the shell (4) in the length direction is moved upward so that the angle between the shell (4) and the horizontal plane reaches a set angle, and at this time, the liquid level of the culture solution in the divided space is higher than the second tube body (9) with a lower height in the divided space; The shell (4) periodically reciprocates at a set angle around the width direction of the shell (4), so that the culture liquid reciprocates along the length direction of the shell (4).

2. The corn tissue culture device according to claim 1, characterized in that: A water absorbing block (11) is placed at the bottom of each cultivation tank to cover the liquid passage hole (13).

3. The corn tissue culture device according to claim 2, characterized in that: The edge of the seedling tray (7) and the inner wall of the accommodating space are slidably sealed and fitted, the seedling tray (7) makes each divided space closed, and exhaust holes (12) are provided at both ends of the length direction of the seedling tray (7), and air purification components are installed on the two exhaust holes (12); The air purification component comprises a first tube body (8), a mounting hole is provided on the shell (4), a second air filter device (3) is mounted on the outer side of the mounting hole, the inner side of the mounting hole is connected to one end of the first tube body (8), and the other end of the first tube body (8) is connected to the top side of the exhaust hole (12).

4. The corn tissue culture device according to claim 3, characterized in that: At least one second air filter device (3) is provided with a shielding cloth for adjusting the air flow resistance in the second air filter device (3).

5. The corn tissue culture device according to claim 3, characterized in that: The cover body (1) is provided with a ventilation hole, and a first air filter device (2) is installed outside the ventilation hole.

6. The corn tissue culture device according to claim 1, characterized in that: Two second tube bodies (9) are mounted on the partition (10), one end of the second tube body (9) is close to the bottom of the partition (10), and the other end of the second tube body (9) is provided with an extension tube extending in a direction away from the bottom of the partition (10), and the extension tubes of the two second tube bodies (9) are located on both sides of the partition (10).

7. The corn tissue culture device according to claim 1, characterized in that: A card slot is provided on the top side of the partition (10), a card block is detachably mounted in the card slot, and both ends of the second tube body (9) penetrate the card block and extend outside the card block.

8. The corn tissue culture device according to claim 1, characterized in that: It also includes a base (6) located below the shell (4), the top side of the base (6) being rotatably connected to the bottom side of the connecting frame, the connecting frame being fixed to the bottom side of the shell (4), and a driving mechanism (5) for driving the shell (4) to rotate around the width direction of the shell (4) being mounted on the base (6).

9. The corn tissue culture device according to claim 1, characterized in that: The cover (1) is made of transparent glass or transparent plastic, and the shell (4) is made of transparent glass or transparent plastic.

10. The corn tissue culture device according to claim 1, characterized in that: The set angle between the housing (4) and the horizontal plane is 5°-40°.

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