Method for establishing rat artificial feeding model

Abstract

The invention discloses a method for establishing a rat artificial feeding model, and belongs to the technical field of biomedicine. The method specifically comprises the following steps: 1) selecting a plurality of newly born SD suckling rats, and averagely dividing the rats into an artificial feeding group and a breast feeding group; 2) preparing artificial milk; each SD suckling mouse in the artificial feeding group is artificially fed through the suckling mouse feeding device; (3) the SD suckling rats and the female rats in the breast feeding group are placed in the same space, and manual intervention is not carried out; and (4) the artificial feeding group and the breast feeding group continue to the weaning period, a weight growth curve of the SD suckling rats is drawn, and if the weight growth curve of the artificial feeding group and the breast feeding group have no obvious statistical difference and the death rate is lower than 0.1%, the artificial feeding suckling rat model is successfully established. The model provides feasible animal model support for research on health outcomes of different life development stages of individuals influenced by environmental factors in early life, and can deeply discuss a potential mechanism of influence of milk powder feeding and breast feeding on individual growth and development.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for establishing an artificial feeding model of rats. Background Art

[0002] The Doha Theory (DOHaD), or the developmental origins of health and disease, explains the impact of environmental factors, especially nutrition, within the first 1,000 days of life on adult health. Many epidemiological studies have found that the risk of certain adult non-communicable diseases is related to it, such as hypertension, type 2 diabetes, cardiovascular disease, and neuropsychiatric disorders.

[0003] It is worth noting that the family upbringing environment is an important environmental factor that affects individual health. According to the theory of early childhood development, early life is a critical period for the establishment of eating behavior. However, the mechanism of the effect of breastfeeding on the growth and development of offspring is still unclear, and there is no unified and standardized exploration model. Therefore, by intervening in the eating habits of suckling mice in early life, an anthropomorphic infant feeding method can be constructed to simulate the early feeding methods of human infants in small mammal groups. Subsequently, by strictly controlling the confounding factors in the growth and development process, we focus on exploring the impact of feeding methods on the occurrence and development of obesity in children, adolescents and adults. Summary of the invention

[0004] The purpose of the present invention is to solve the deficiencies in the prior art and provide a method for establishing an artificial feeding model for rats.

[0005] The specific technical solutions adopted by the present invention are as follows:

[0006] The present invention provides a method for establishing an artificial feeding model of rats, and the specific steps are as follows:

[0007] S1: Several newborn SD rats were selected and divided equally into an artificial feeding group and a breast feeding group;

[0008] S2: Prepare artificial milk; feed each SD rat in the artificial feeding group artificially using the rat feeding device: first suck the artificial milk into the rat feeding device, empty the air inside and then gavage the SD rat; during the feeding process, ensure that the head, neck and body of the SD rat are in a straight line, and the head is slightly higher than the body; the amount of artificial milk fed each time Where: W is the weight of SD rats, in grams, H is the feeding amount, in mL;

[0009] After the scheduled feeding is completed, observe the SD rat for 30-40 seconds. If the body color and breathing are normal, continue to feed the next SD rat until all the SD rats in the artificial feeding group have been fed.

[0010] S3: The SD rats and mother rats in the breast-feeding group were placed in the same room without human intervention;

[0011] S4: Both the artificial feeding group and the breast feeding group continued until the SD rat weaning period, and the weight growth curves of the SD rats in the artificial feeding group and the breast feeding group were drawn. If the weight growth curve of the artificial feeding group is not lower than that of the breast feeding group, and the mortality rate of the SD rats in the artificial feeding group is lower than 0.1%, the offspring obese rat model is successfully established.

[0012] Preferably, the suckling mouse feeding device is made by connecting a medical peripherally inserted central venous catheter with a diameter of 0.62 mm and a disposable medical sterile syringe needle.

[0013] Preferably, goat milk powder is dissolved in sterilized water to prepare artificial milk, wherein the concentration of goat milk powder is 300-350 g / L; the prepared artificial milk needs to be kept in the range of 35-40° C. to avoid indigestion in suckling mice due to too low temperature of artificial milk.

[0014] Preferably, each SD rat in the artificial feeding group is fed 5 to 6 times a day, with an interval of 1.5 to 2 hours between each feeding.

[0015] Preferably, during the daily feeding period of the artificial feeding group, the abdomen of each SD rat was massaged to stimulate urination and defecation 3 to 4 times.

[0016] Preferably, both the artificial feeding group and the breastfeeding group are continued for 28 days.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) The present invention innovatively proposes a method for artificial feeding of newborn SD mice. After the start of artificial feeding, the weight and mortality of the experimental animals are tracked and recorded every day, and their general physiological activity status, such as appearance, hair and mental state, is observed and recorded; then, by drawing a weight growth curve and comparing the weight change trends of the SD mice in the breast-fed group and the artificial-fed group, it is found that the weight growth curve of the artificial-fed group is not lower than that of the breast-fed group, and the mortality rate of the SD mice in the artificial-fed group is lower than 0.1%.

[0019] (2) The present invention utilizes the above-mentioned artificial feeding method to simulate the animal model establishment method of the early human infant feeding environment. It is found that the artificially fed mice have good growth and development from the fetal period to adulthood, and there is no significant difference in body weight, survival rate, etc. compared with the breast-fed offspring. This provides a feasible animal model support for the impact of early life environmental factors on different health outcomes of individuals, and has theoretical significance and application value for in-depth research on the impact of breastfeeding and different family rearing environments on the occurrence and development of health outcomes at different stages of life. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A flow chart of the method for establishing the artificial feeding model of rats provided by the present invention;

[0021] Figure 2 This is the weight curve of SD rats in the artificial feeding group and breast-feeding group at different growth and development stages;

[0022] Figure 3 This is the body length curve of SD rats in the artificial feeding group and breastfeeding group at different growth and development stages. DETAILED DESCRIPTION

[0023] The present invention is further described and illustrated below in conjunction with the accompanying drawings and specific embodiments. The technical features of each embodiment of the present invention can be combined accordingly without conflicting with each other.

[0024] Example

[0025] This embodiment provides a method for establishing an artificial feeding model in rats, and the specific steps are as follows: Figure 1 ):

[0026] (1) A peripherally inserted central venous catheter (PICC tube) with a diameter of 0.62 mm and a 1 mL disposable medical sterile syringe needle were connected to make a suckling mouse feeding device.

[0027] (2) A number of newborn SD mice were selected and divided equally into an artificial feeding group and a breast feeding group.

[0028] (3) Weigh the SD rats in the artificial feeding group in advance. Prepare an appropriate amount of artificial milk by dissolving 1g of goat milk powder in 3ml of sterile high-pressure water. After preparation, place the artificial milk on a heat pad and keep it in the range of 35-40℃ to avoid indigestion caused by the artificial milk being too low in temperature.

[0029] (4) Artificial feeding was performed on each SD rat in the artificial feeding group using a rat feeding device: a syringe filled with artificial milk was placed in a warm water bag, and the SD rat's forelimbs were then fixed by hand so that its head was in the mouth of the feeder, with its head, neck and body in a straight line and the head slightly higher than the body.

[0030] Suck artificial milk into the suckling mouse feeding device, empty the internal air and perform gavage on the SD suckling mouse: first drop a small amount of artificial milk on the lips of the SD suckling mouse to induce it to open its mouth and lick, then gently insert the tube into the mouth of the SD suckling mouse, and let the PICC tube go straight into the esophagus to the stomach as the SD suckling mouse sucks, then slowly push the artificial milk in until the predetermined amount is reached, and then slowly and steadily pull out the PICC tube.

[0031] After the predetermined amount of feeding is completed, the SD rat is observed for 30-40 seconds. If the body color and breathing are normal, the next SD rat is fed until all the SD rats in the artificial feeding group are fed.

[0032] Artificial feeding was performed 6 times a day at 9:00, 10:30, 12:00, 13:30, 15:00, and 16:30. The amount of artificial milk fed each time was Where: W is the body weight of SD rats, in grams; H is the feeding amount, in mL.

[0033] In the artificial feeding group, the abdomen of each SD rat was massaged three times during the daily feeding period to stimulate urination and defecation.

[0034] (5) The SD rats and mother rats in the breast-feeding group were placed in the same room without any human intervention.

[0035] (6) The artificial feeding group and the breast feeding group were continued until the 28th day of the SD rat weaning period, and the weight growth curves of the SD rats in the artificial feeding group and the breast feeding group were drawn.

[0036] In this embodiment, if the weight growth curve of the artificial feeding group within 28 days is not lower than that of the breast feeding group, and the mortality rate of SD suckling mice in the artificial feeding group is lower than 0.1% (the mortality rate of the breast feeding group is 0.25%), it is determined that the artificial feeding suckling mouse model is successfully established. This shows that the artificial feeding method provided by the present invention is operationally feasible and has data support.

[0037] In order to verify the weight changes of the artificially reared and breast-fed mice in adulthood, the mice in the artificially reared and breast-fed groups were housed in separate cages 28 days later. The feeding methods were as follows: the weaned mice were randomly divided into groups in a simple manner and fed together in cages with four mice per cage. They were supplied with sufficient ordinary feed and autoclaved water every day to ensure their free diet and normal circadian rhythm.

[0038] Figure 2 The weight curves of SD rats in the artificial feeding group and breast-feeding group at different growth and development stages. Figure 2 It can be seen that the weight of the artificially fed group of mice was slightly higher than that of the breast-fed group from the late weaning period (i.e. 21 days of age). This shows that there was no significant difference in weight gain between the artificially fed group and the breast-fed group from infancy to adulthood, and the weight was slightly higher than that of the breast-fed group, indicating that their growth and development were good.

[0039] Figure 3 The body length curves of SD rats in the artificial feeding group and breast-feeding group at different growth and development stages. Figure 3It can be seen that there was no significant difference in body length between the artificially fed group and the breast-fed group after weaning (i.e., 28 days of age). This shows that there was no significant difference in body length growth between the artificially fed group and the breast-fed group from the late infancy to adulthood, indicating that their height development and growth were good.

[0040] The above-described embodiment is only a preferred solution of the present invention, but it is not intended to limit the present invention. A person skilled in the relevant technical field may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, any technical solution obtained by equivalent replacement or equivalent transformation falls within the protection scope of the present invention.

Claims

1. A method for establishing an artificial feeding model of rats, characterized in that: The specific steps are as follows: S1: Several newborn SD rats were selected and divided equally into an artificial feeding group and a breast feeding group; S2: Prepare artificial milk; feed each SD rat in the artificial feeding group artificially using the rat feeding device: first suck the artificial milk into the rat feeding device, empty the air inside and then gavage the SD rat; during the feeding process, ensure that the head, neck and body of the SD rat are in a straight line, and the head is slightly higher than the body; the amount of artificial milk fed each time Where: W is the weight of SD rats, in grams, H is the feeding amount, in mL; After the scheduled feeding is completed, observe the SD rat for 30-40 seconds. If the body color and breathing are normal, continue to feed the next SD rat until all the SD rats in the artificial feeding group have been fed. S3: The SD rats and mother rats in the breast-feeding group were placed in the same room without human intervention; S4: Both the artificial feeding group and the breast feeding group continued until the SD rat weaning period, and the weight growth curves of the SD rats in the artificial feeding group and the breast feeding group were drawn. If the weight growth curve of the artificial feeding group is not lower than that of the breast feeding group, and the mortality rate of the SD rats in the artificial feeding group is lower than 0.1%, the offspring obese rat model is successfully established.

2. The method for establishing a rat artificial feeding model according to claim 1, characterized in that, The suckling mouse feeding device is made by connecting a medical peripheral puncture central venous catheter with a diameter of 0.62 mm and a disposable medical sterile syringe needle.

3. The method for establishing a rat artificial feeding model according to claim 1, characterized in that, Goat milk powder is dissolved in sterilized water to prepare artificial milk, wherein the concentration of goat milk powder is 300-350 g / L; the prepared artificial milk needs to be kept within the range of 35-40°C to avoid indigestion caused by the artificial milk being too low in temperature.

4. The method for establishing a rat artificial feeding model according to claim 1, characterized in that: Each SD rat in the artificial feeding group was fed 5 to 6 times a day, with an interval of 1.5 to 2 hours between each feeding.

5. The method for establishing a rat artificial feeding model according to claim 1, characterized in that, During the daily feeding period in the artificial feeding group, the abdomen of each SD rat was massaged to stimulate urination and defecation 3 to 4 times.

6. The method for establishing a rat artificial feeding model according to claim 1, characterized in that: Both the artificial feeding group and the breastfeeding group lasted for 28 days.

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