Multi-effect plant growth regulator
Through the combination of glutamectin with berberine sulfate or thiazole zinc, the drug resistance risk caused by long-term use of glutamectin is solved, and multiple effects on plant diseases are achieved. .
Patent Information
- Application Number
- CN202411576229.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-06
- Publication Date
- 2025-02-14
AI Technical Summary
Long-term use of glutamectin alone develops resistance to plant pathogenic bacteria, and there is a risk of resistance.
By combining glutamectin with the bactericidal active ingredient berberine sulfate or thiazole zinc, a multiple-effect plant growth regulator is formed to reduce the risk of drug resistance.
It improves the prevention and treatment effect of plant diseases, broadens the complex combination of glutamectin, and reduces its drug resistance risk, so that plant growth adjustment has multiple effects.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of agricultural production, and in particular relates to a multi-effect plant growth regulator. Background Art
[0002] Guvermectin (GV) is a new plant growth regulator developed by Northeast Agricultural University, CAS: 2004-04-8, molecular formula: C 11 H 13 N5O4, the structural formula is as follows:
[0003]
[0004] Oxyprolin is a nucleoside compound produced by fermentation of the endophytic actinomycete NEAU6 from the traditional Chinese medicine Paris polyphylla. As a plant growth regulator, the main mechanism of action of oryzanthin is to promote the bifurcation of plant roots, with a lasting effect of up to 60 days. It has the characteristics of making plants resistant to stress, lodging and disease. In terms of application, oryzanthin can promote rice seedling emergence, promote root growth, increase the number of tillers, significantly increase the number of effective panicles and rice grains, and has a significant effect on increasing yield.
[0005] In addition, the prior art discloses that oryzanin has good antibacterial activity against plant pathogenic bacteria, and exerts antibacterial effects by inhibiting the key enzyme GMPs in bacterial guanine synthesis, and can effectively prevent and control devastating diseases such as rice bacterial blight, tomato bacterial wilt, and kiwifruit canker, and has inhibitory effects on its pathogens Xanthomonas oryzae, tomato bacterial wilt, and kiwifruit canker. It can be seen that oryzanin can not only be used as a plant growth regulator, but also has the potential to be used as a fungicide in agricultural production, further broadening its application field.
[0006] Although oryzanin has good antibacterial activity against plant pathogenic bacteria, if used alone for a long time, it is easy to cause pathogenic bacteria to develop resistance to it, and there is a certain risk of drug resistance. For this reason, oryzanin needs to be compounded to reduce its risk of drug resistance. Summary of the invention
[0007] The object of the present invention is to provide a multi-effect plant growth regulator which can reduce the risk of oryzanol resistance.
[0008] The present invention provides the following technical solutions:
[0009] A multi-effect plant growth regulator is prepared from active ingredients and auxiliary ingredients. The active ingredients are compounded from oryzanol and a fungicide active ingredient. The fungicide active ingredient is berberine sulfate or zinc thiazole.
[0010] Preferably, the mass of the active ingredient accounts for 0.05-90% of the total mass of the multi-effect plant growth regulator.
[0011] Preferably, the mass of the active ingredient accounts for 0.5-45% of the total mass of the multi-effect plant growth regulator.
[0012] Preferably, the auxiliary ingredients are ingredients allowed to be added in pesticide science.
[0013] Preferably, the auxiliary ingredients are selected from one or more of emulsifiers, wetting agents, dispersants, fillers, solvents, penetrants, adhesives, stabilizers, synergists, defoamers, antifreeze agents, protective agents and warning colors.
[0014] Preferably, the composite mass ratio of oryzanthin to berberine sulfate is 1-3:30-1.
[0015] Preferably, the compounding mass ratio of oryzanol and zinc thiazole is 1-9:9-1.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] The multi-effect plant growth regulator of the present invention is compounded by oryzanol, bactericidal berberine sulfate and zinc thiazole, and the compounded combination shows a synergistic effect on plant diseases in a mass ratio of 1-3:30-1 and 1-9:9-1, respectively, which can improve the prevention and control effect of the disease, expand the compounded combination of oryzanol and reduce its drug resistance risk. In addition, in the process of preventing and controlling plant diseases, oryzanol can also play the role of a plant growth regulator, regulate and enhance plant stress resistance, disease resistance and promote plant growth, etc., so that the plant growth regulator of the present invention has multiple functions of preventing and controlling diseases and regulating plant growth. DETAILED DESCRIPTION
[0018] The following examples are provided to illustrate the present invention in detail. The following examples are provided to better illustrate the present invention and are not intended to limit the scope of the present invention. The materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.
[0019] Example 1: Indoor activity test
[0020] The experiment of this embodiment is roughly operated as follows: the Oxford cup method is used to determine the growth inhibition rate of the target bacteria by the single agent and the compound mixture, and the logarithmic value of the agent concentration and the probability value of the growth inhibition rate are regressed and analyzed, and the inhibitory effect of the agent on the medium concentration EC is calculated. 50 , the Wadley method was used to evaluate the type of combined action of the compound combination on the target bacteria.
[0021] 1. Test bacteria
[0022] The test bacteria in this example are Xanthomonas oryzae pv. oryzicola isolated from diseased rice plants with typical symptoms of rice bacterial leaf streak disease.
[0023] Rice bacterial leaf streak is an important quarantine bacterial disease caused by Xanthomonas oryzae pv. Oryzicola, which is characterized by epidemic, explosive and destructive nature. The lesions initially appear as dark green water-soaked translucent spots, which gradually expand along the veins. When expanding, they are restricted by the veins. The lesions appear as thin lines or short dashed lines, and are yellow-brown. When the field humidity is high, honey-yellow bacterial pus overflows from the surface of the diseased part, which is in the shape of dewdrops. After drying, it appears as small yellow gum-like particles, shaped like dotted lines, and is not easy to fall off. When the disease is severe, the stripes merge into irregular yellow-brown to dry white large patches.
[0024] 2. Culture medium
[0025] NA medium: beef extract 10 g, peptone 10 g, glucose 20 g, agar 17 g, distilled water 1 L, pH 7.0;
[0026] NB medium: beef extract 10 g, peptone 10 g, glucose 20 g, distilled water 1 L, pH 7.0;
[0027] The above culture media were sterilized at 121°C and 0.1 MPa for 20 min before use.
[0028] 3. Test reagents
[0029] 94% oryzanol technical (Fujian Kaili Biological Products Co., Ltd.), 75% berberine sulfate technical (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.), 95% thiazole zinc technical (Zhejiang Xinnong Chemical Co., Ltd.)
[0030] According to the needs of the test, the test agent was first dissolved in dimethyl sulfoxide, and then diluted with a surfactant 0.1% Triton-X100 aqueous solution to a single-dose stock solution with an active ingredient concentration of 5000 mg / L. The single-dose stock solution was then diluted with a 0.1% Triton-X100 aqueous solution into 5 series of mass concentrations. In order to explore the type of combined action of the compound agent on the test bacteria, it is also necessary to prepare multiple groups of mixed agents with the single-dose stock solution of oryzanol, the single-dose stock solution of berberine sulfate, and the single-dose stock solution of zinc thiazole. Each group of mixed agents is also diluted with a 0.1% Triton-X100 aqueous solution into 5 series of mass concentrations. The above agents are prepared and used immediately.
[0031] 4. Test methods
[0032] The Oxford cup method was used. Before the test, the test bacteria needed to be cultured and prepared into a bacterial suspension, as follows: the test bacteria (Xanthomonas oryzae pathogenic bacteria) were selected and cultured on NA medium at 28°C for 24 hours, and then 2 loops of colonies were selected and inoculated into NB medium, and cultured at 28°C and 180r / min with shaking until the OD 600 The value was adjusted to 0.6, and then the concentration of bacterial suspension was diluted to 2×10 8 cfu / mL.
[0033] When the sterilized NA medium is cooled to about 50°C, pour it into a 9cm diameter culture dish, 15mL per dish, and cool to form a NA plate. Spread 100μL of bacterial suspension evenly on the NA plate, and place a 6mm diameter Oxford cup in the center of the plate. Add 100μL of the test solution to the Oxford cup. The treatment with an equal amount of 0.1% Triton-X100 aqueous solution is used as a blank control. Each treatment is repeated 3 times, and each repetition has 3 plates. Cover the treated plate with a dish cover and place it in a 28°C bacterial incubator for culture. After 36 hours, measure the diameter of the inhibition zone using the cross method, take the average value, and calculate the colony growth inhibition rate.
[0034]
[0035] 5. Data Analysis
[0036] Linear regression analysis was performed with the logarithmic value of the drug concentration as x and the corresponding inhibition rate probability value as y to obtain the regression equation and the EC of each treatment drug. 50 .
[0037] The synergistic coefficient SR of the mixture was calculated according to the Wadley method, and the synergistic effect of the mixed drugs was evaluated based on the synergistic coefficient SR: SR greater than 1.5 is a synergistic effect, SR less than 0.5 is an antagonistic effect, and SR between 0.5 and 1.5 is an additive effect. Results are shown in Table 1-3.
[0038] Table 1 Toxicity of oryzanol, berberine sulfate and zinc thiazole to rice bacterial leaf streak pathogen
[0039]
[0040]
[0041] Table 2 Effect of oryzanol and berberine sulfate on bacterial leaf streak pathogen of rice
[0042]
[0043] Table 3 The effect of oryzanol and zinc thiazole on rice bacterial leaf streak pathogen
[0044]
[0045]
[0046] As shown in Table 1-3, when the compounding mass ratio of oryzanol and berberine sulfate is 1-3:30-1, it shows a synergistic effect on the growth of rice bacterial leaf streak pathogen; when the compounding mass ratio of oryzanol and zinc thiazole is 1-9:9-1, it shows a synergistic effect on the growth of rice bacterial leaf streak pathogen. This shows that when oryzanol and the bactericidal active ingredients berberine sulfate and zinc thiazole in the plant growth regulator of the present invention are compounded, the control effect of plant diseases can be improved, and the compounding combination of oryzanol can be broadened.
Claims
1. A multi-effect plant growth regulator, characterized in that: The multi-effect plant growth regulator is prepared from active ingredients and auxiliary ingredients. The active ingredients are compounded from oryzanol and bactericidal active ingredients. The bactericidal active ingredients are berberine sulfate or zinc thiazole.
2. The multi-effect plant growth regulator according to claim 1, characterized in that: The mass of the active ingredients accounts for 0.05-90% of the total mass of the multi-effect plant growth regulator.
3. The multi-effect plant growth regulator according to claim 2, characterized in that: The mass of the active ingredients accounts for 0.5-45% of the total mass of the multi-effect plant growth regulator.
4. The multi-effect plant growth regulator according to claim 1, characterized in that: The auxiliary ingredients are ingredients allowed to be added in pesticide science.
5. The multi-effect plant growth regulator according to claim 4, characterized in that: The auxiliary components are selected from one or more of emulsifiers, wetting agents, dispersants, fillers, solvents, penetrants, adhesives, stabilizers, synergists, defoamers, antifreeze agents, protective agents and warning colors.
6. The multi-effect plant growth regulator according to claim 1, characterized in that: The composite mass ratio of oryzanol to berberine sulfate is 1-3:30-1.
7. The multi-effect plant growth regulator according to claim 1, characterized in that: The compounding mass ratio of oryzanol and zinc thiazole is 1-9:9-1.