Cell activity real-time monitoring equipment and monitoring method

Abstract

The invention belongs to the technical field of detection, particularly relates to optical means, and particularly relates to real-time monitoring equipment and a monitoring method for cell activity. The invention provides a cell activity real-time monitoring device, which comprises: a detection device, which is suitable for detecting liquid in a test tube; the injector is arranged on the side wall of the detection device in a lifting manner, and an injection head is arranged at the lower end of the injector in a lifting manner; after the test tube moves to the position below the injector, the injector moves downwards until the lower bottom wall of the injection head is attached to the liquid level in the test tube; the injection head shrinks towards the injector in the process of being inserted into the liquid level so as to discharge bubbles at the lower end of the injection head; after the depth of the injection head inserted into the liquid level reaches a preset value, the injector is suitable for injecting detection liquid into the test tube. Bubbles at the lower end of the injection head can be discharged through cooperation of the injection head and the injector.

Description

Technical Field

[0001] The present invention belongs to the field of detection technology, and specifically relates to the use of optical means, and in particular to a real-time monitoring device and a monitoring method for cell viability. Background Art

[0002] A cell culture fluid is stored in the test tube. When the cell culture fluid is tested, a specific reaction or change can be observed by adding a test fluid to the cell culture fluid in the test tube, thereby obtaining a test structure.

[0003] In the related art, the test solution is usually injected into the culture solution by inserting a syringe. However, bubbles will be generated during the process of inserting the syringe into the culture solution, and the generation of bubbles will affect the accuracy of subsequent observation and detection.

[0004] Therefore, how to avoid the generation of bubbles during the process of inserting the injection tube into the liquid surface in the test tube is a technical problem that urgently needs to be solved in this field.

[0005] It should be noted that the above information disclosed in this background technology section is only used to understand the background technology of the present application concept, and therefore, the above description is not considered to constitute information of the prior art. Summary of the invention

[0006] The embodiments of the present disclosure at least provide a real-time cell viability monitoring device and a monitoring method thereof.

[0007] In a first aspect, an embodiment of the present disclosure provides a device for real-time monitoring of cell viability, comprising: A detection device, which is suitable for detecting the liquid in the test tube; A syringe, which is arranged on the side wall of the detection device and has an injection head arranged on the lower end; After the test tube moves to the bottom of the syringe, the syringe moves downward until the bottom wall of the injection head fits the liquid surface in the test tube; The injection head is retracted toward the syringe during the process of being inserted into the liquid surface to discharge the air bubbles at the lower end of the injection head; After the depth of the injection head inserted into the liquid surface reaches a preset value, the syringe is suitable for injecting the detection liquid into the test tube.

[0008] In an optional embodiment, the syringe comprises: a barrel, which is arranged above the test tube and is hollow inside; The piston disc is arranged in the cylinder body and is slidably sealed with the inner wall of the cylinder body; The injection head is arranged at the bottom of the cylinder body, and the lower end thereof protrudes from the bottom wall of the cylinder body; The piston disc moves away from the injection head to absorb the test liquid into the cylinder under negative pressure; The injection head is pushed by buoyancy to retract and move into the cylinder, and the test liquid flows downward to discharge bubbles formed by the bottom of the injection head contacting the liquid surface in the test tube.

[0009] In an optional embodiment, the injection head is conical; The bottom wall of the injection head is provided with a plurality of exhaust grooves in the radial direction, and the exhaust grooves are suitable for guiding the bubbles to overflow toward the outer wall of the injection head.

[0010] In an optional embodiment, a plurality of slide grooves are formed at equal intervals along the axial direction on the outer wall of the injection head, a support block is slidably disposed in each of the slide grooves, the support block extends radially along the injection head, and the support block protrudes from the outer wall of the barrel.

[0011] In an optional embodiment, a buoyancy ring is provided on the outside of the injection head, and the inner wall of the buoyancy ring is arranged at the outer end of the support block. The buoyancy ring is suitable for detecting whether the depth of the injection head inserted into the liquid surface reaches a preset value.

[0012] In an optional embodiment, the outer wall of the cylinder is provided with a plurality of communication holes at equal intervals along the axial direction, and one communication hole corresponds to one support block; The buoyancy ring is suitable for driving the support block to be inserted into the communicating hole to detect whether the depth of the detection head inserted into the liquid surface reaches a preset value.

[0013] In an optional embodiment, a spring is disposed in each of the communicating holes, and the spring is suitable for abutting against the supporting block; The injection head is inserted into the liquid surface in the test tube, and the buoyancy ring moves upward due to the buoyancy of the liquid surface in the test tube until the spring contacts the support block; If the liquid level in the test tube overflows the buoyancy ring, the depth of the injection head inserted into the test tube reaches the specified depth, and the piston plate moves downward to inject the test liquid in the cylinder into the test tube.

[0014] In an optional embodiment, it includes: a syringe, which is lifted and arranged above the test tube and is suitable for injecting liquid into the test tube; The syringe comprises: a barrel, which is arranged above the test tube and is hollow inside; The piston disc is arranged in the cylinder body and is slidably sealed with the inner wall of the cylinder body; An injection head is arranged at the bottom of the cylinder body and its lower end protrudes from the bottom wall of the cylinder body; The piston disc moves away from the injection head to absorb the test liquid into the cylinder under negative pressure; The injection head is pushed by buoyancy to retract and move into the cylinder, and the test liquid flows downward to discharge bubbles formed by the bottom of the injection head contacting the liquid surface in the test tube.

[0015] In an optional embodiment, the injection head is conical; The bottom wall of the injection head is provided with a plurality of exhaust grooves in the radial direction, and the exhaust grooves are suitable for guiding the bubbles to overflow toward the outer wall of the injection head.

[0016] In an optional embodiment, a plurality of slide grooves are provided at equal intervals along the axial direction on the outer wall of the injection head, a support block is slidably arranged in each of the slide grooves, the support block extends radially along the injection head, and the support block protrudes from the outer wall of the cylinder body; a buoyancy ring is provided on the outside of the injection head, the inner wall of the buoyancy ring is arranged at the outer end of the support block, and the buoyancy ring is suitable for detecting whether the depth of the injection head inserted into the liquid surface reaches a preset value.

[0017] In an optional embodiment, the outer wall of the cylinder is provided with a plurality of communication holes at equal intervals along the axial direction, and one communication hole corresponds to one support block; A spring is disposed in each of the communicating holes, and the spring is suitable for abutting against the supporting block; The injection head is inserted into the liquid surface in the test tube, and the buoyancy ring moves upward due to the buoyancy of the liquid surface in the test tube until the spring contacts the support block; If the liquid level in the test tube overflows the buoyancy ring, the depth of the injection head inserted into the test tube reaches the specified depth, and the piston plate moves downward to inject the test liquid in the cylinder into the test tube.

[0018] In a second aspect, an embodiment of the present disclosure further provides a monitoring method, the monitoring method comprising: After the test tube moves to the bottom of the syringe, the syringe moves downward until the bottom wall of the injection head fits the liquid surface in the test tube; The injection head is retracted toward the syringe during the process of being inserted into the liquid surface to discharge the air bubbles at the lower end of the injection head; After the depth of the injection head inserted into the liquid surface reaches a preset value, the syringe is suitable for injecting the detection liquid into the test tube.

[0019] The beneficial effect of the present invention is that the real-time monitoring device for cell viability, i.e., the monitoring method, cooperates with the injection head and the liquid surface in the test tube. During the process of the injection head being gradually inserted into the liquid surface in the test tube, the liquid in the barrel flows downward to discharge bubbles formed by the bottom of the injection head contacting the liquid surface in the test tube. During the process of the injection head being inserted into the liquid surface, the depth of the injection head inserted into the liquid surface in the test tube is determined by observing the relative position relationship between the buoyancy ring and the liquid surface, so that the detection liquid injected into the test tube from the barrel can reach the optimal injection depth.

[0020] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention are realized and obtained by the structures particularly pointed out in the description, claims and drawings.

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, this article specifically cites preferred embodiments and provides detailed descriptions as follows in conjunction with the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 A three-dimensional diagram of a device for real-time monitoring of cell viability provided in an embodiment of the present disclosure; Figure 2 A stereoscopic diagram of a syringe and a test tube provided for an embodiment of the present disclosure; Figure 3 A perspective view of a syringe provided by an embodiment of the present disclosure; Figure 4 A cutaway perspective view of a syringe and a test tube provided in an embodiment of the present disclosure; Figure 5 A schematic diagram of the injection head moving upward provided in an embodiment of the present disclosure.

[0024] In the figure: 2. Syringe; 21. Cylinder; 22. Piston disc; 23. Connecting hole; 24. Spring; 3. Injection head; 31. Exhaust groove; 32. Slide groove; 33. Support block; 34. Buoyancy ring; 4. Detection device; 5. Test tube. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] In this article, when it is mentioned that the first component is located on the second component, this may mean that the first component may be directly formed on the second component, or the third component may be interposed between the first component and the second component. In addition, in the drawings, in order to effectively describe the technical content, the thickness of the components may be exaggerated or reduced.

[0027] Herein, example embodiments of the present disclosure will be described in more detail with reference to the accompanying drawings. As used herein, expressions such as "at least one of..." modify the entire list of elements when following a list of elements, rather than modifying individual elements in the list. For example, the expression "at least one of a, b, and c" should be understood to include only a, only b, only c, both a and b, both a and c, both b and c, or all of a, b, and c.

[0028] The terms used herein are only used to describe specific exemplary configurations and are not intended to be limited. As used herein, the singular articles "one", "an" and "the" may also be intended to include plural forms, unless it is clearly indicated above that this is not the case. The terms "comprise", "include" and "have" are inclusive, and therefore specify the presence of features, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, steps, operations, elements, components and / or combinations thereof. The method steps, processes and operations described herein should not be interpreted as necessarily requiring them to be performed in the specific order discussed or shown, unless specifically identified as an execution order. Additional or alternative steps may be adopted.

[0029] As used herein, the phrases "in one embodiment," "according to one embodiment," "in some embodiments," and the like generally refer to the fact that the particular feature, structure, or characteristic following the phrase may be included in at least one embodiment of the present disclosure. Therefore, a particular feature, structure, or characteristic may be included in more than one embodiment of the present disclosure, so that these phrases do not necessarily refer to the same embodiment. As used herein, the terms "example," "exemplary," and the like are used to "serve as an example, instance, or illustration." Any implementation, aspect, or design described herein as "example" or "exemplary" is not necessarily to be construed as preferred or superior to other implementations, aspects, or designs. On the contrary, the use of the terms "example," "exemplary," and the like is intended to present concepts in a concrete manner.

[0030] After research, it was found that the disadvantages of the existing technology are: cell culture fluid is stored in the test tube. When the cell culture fluid is tested, by adding the test fluid to the cell culture fluid in the test tube, a specific reaction or change can be observed, thereby obtaining a test structure. For example, in the antibody-producing cell function test, the spleen cell suspension is mixed with SRBC (goat red blood cells) and complement and added to the test tube, and the immune function is evaluated by observing the hemolytic reaction. In addition, in other experiments, such as serum hemolysin determination, the immune response is also evaluated by adding a specific test fluid to the test tube.

[0031] The conventional practice is to drop the test solution directly into the culture medium, but this method will cause the test solution to splash toward the inner wall of the test tube when the droplets fall, and the fusion time is long.

[0032] In the related art, the test liquid is usually injected into the culture liquid by inserting a syringe, which can not only prevent splashing but also facilitate the mixing of the liquid. However, bubbles will be generated during the process of inserting the syringe into the culture liquid, and the generation of bubbles will affect the accuracy of subsequent observation and detection.

[0033] Therefore, how to avoid the generation of bubbles is a technical problem that needs to be solved urgently in this field.

[0034] The defects existing in the above solutions are the results obtained by the inventor after practice and careful research. Therefore, the discovery process of the above problems and the solutions proposed by the present invention in this article for the above problems should be the contributions made by the inventor to the present invention during the disclosure process.

[0035] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0036] Some embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0037] like Figures 1 to 5 As shown, some embodiments provide a real-time monitoring device for cell viability, comprising: The detection device 4 is suitable for detecting the liquid in the test tube 5; the syringe 2 is arranged on the side wall of the detection device 4, and an injection head 3 is arranged at the lower end; the syringe 2 injects the detection liquid into the test tube 5, so that the detection device 4 can detect the liquid in the test tube 5. The syringe 2 is arranged vertically, and the syringe 2 is suitable for vertically moving up and down relative to the test tube 5. After the test tube 5 moves below the syringe 2, the syringe 2 moves downward until the lower bottom wall of the injection head 3 is in contact with the liquid surface in the test tube 5; the injection head 3 shrinks toward the syringe 2 during the insertion into the liquid surface to discharge the bubbles at the lower end of the injection head 3; after the depth of the injection head 3 inserted into the liquid surface reaches a preset value, the syringe 2 is suitable for injecting the detection liquid into the test tube 5. Through the cooperation between the injection head 3 and the liquid surface in the test tube 5, during the gradual insertion of the injection head 3 into the liquid surface in the test tube 5, the liquid in the cylinder 21 flows downward to discharge the bubbles formed by the bottom of the injection head 3 due to the contact with the liquid surface in the test tube 5. When the injection head 3 is inserted into the liquid surface, the relative position relationship between the buoyancy ring 34 and the liquid surface is observed to determine the depth of the injection head 3 inserted into the liquid surface of the test tube 5, so that the detection liquid injected into the test tube 5 by the barrel 21 can reach the optimal injection depth.

[0038] Reference Figure 3The syringe 2 comprises: a barrel 21, which is arranged above the test tube 5 and is hollow inside; the outer diameter of the barrel 21 is smaller than the inner diameter of the test tube 5, and the barrel 21 is suitable for moving up and down in the test tube 5. Further, the detection device 4 is provided with a support bracket, and the support bracket is provided with a lifting device, the barrel 21 is fixed at the movable end of the lifting device, and the lifting device is suitable for driving the barrel 21 to move vertically up and down. When the test tube 5 moves below the barrel 21, the lifting device is suitable for driving the barrel 21 to move into the test tube 5 until the injection head 3 is close to the liquid surface in the test tube 5. The piston disc 22 is arranged in the barrel 21 for lifting and lowering, and is slidably sealed with the inner wall of the barrel 21; a piston rod is fixed on the upper end of the piston disc 22, and the piston rod is fixed at the movable end of the driving cylinder, and the piston rod is suitable for pushing the piston disc 22 to move up and down in the barrel 21, so as to achieve the purpose of sucking the detection liquid into the barrel 21, or injecting the detection liquid in the barrel 21 into the test tube 5. The injection head 3 is arranged at the bottom of the cylinder 21, and the lower end protrudes from the bottom wall of the cylinder 21; wherein the piston disc 22 moves in the direction away from the injection head 3 to absorb the negative pressure of the detection liquid into the cylinder 21; after the height of the detection liquid in the cylinder 21 reaches the set height, the lifting device is suitable for driving the cylinder 21 to move downward until the bottom wall of the injection head 3 is close to the liquid surface in the test tube 5, and further, the injection head 3 is pushed by the buoyancy of the liquid surface in the test tube 5 to shrink and move into the cylinder 21, and the detection liquid flows downward to discharge the bubbles formed by the bottom of the injection head 3 due to the contact with the liquid surface in the test tube 5. If the bubbles are not eliminated, when the injection head 3 injects the detection liquid into the liquid surface in the test tube 5, bubbles will be formed in the liquid in the test tube 5, and the generation of bubbles is not only not conducive to the growth of cells, but also affects the accuracy of the subsequent detection device 4 in detecting the liquid in the test tube 5.

[0039] Reference Figure 4 , the injection head 3 is conical; the inner diameter of the water outlet of the bottom wall of the injection head 3 is smaller than the inner diameter of the cylinder 21. The bottom wall of the injection head 3 is provided with a plurality of exhaust grooves 31 in the radial direction, and the exhaust grooves 31 are suitable for guiding the bubbles to overflow toward the outer wall of the injection head 3. When the injection head 3 is pushed by the buoyancy to shrink and move into the cylinder 21, a small part of the detection liquid in the cylinder 21 will flow downward from the water outlet of the injection head 3. The flowing detection liquid is suitable for driving the bubbles at the bottom of the injection head 3 to overflow from each exhaust groove 31, thereby preventing the bubbles from entering and being retained in the liquid in the test tube 5.

[0040] Reference Figure 3The outer wall of the injection head 3 is provided with a plurality of slide grooves 32 at equal intervals in the axial direction, and a support block 33 is slidably arranged in each of the slide grooves 32. The support block 33 extends radially along the injection head 3, and the support block 33 protrudes from the outer wall of the cylinder 21. A buoyancy ring 34 is sleeved on the outside of the injection head 3. The buoyancy ring 34 is hollow inside, and the outer diameter of the buoyancy ring 34 is smaller than the inner diameter of the test tube 5. When the buoyancy ring 34 contacts the liquid in the test tube 5, the buoyancy ring 34 is subjected to the buoyancy of the liquid and moves upward relative to the injection head 3. The inner wall of the buoyancy ring 34 is arranged at the outer end of the support block 33, wherein the injection head 3 is inserted into the liquid surface until the liquid surface overflows the buoyancy ring 34, and it is determined that the depth of the injection head 3 inserted into the liquid surface reaches a preset value.

[0041] Reference Figure 4 and Figure 5 The outer wall of the cylinder 21 is provided with a plurality of connecting holes 23 at equal intervals in the axial direction, and one connecting hole 23 corresponds to one supporting block 33; the supporting block 33 is suitable for being inserted into the connecting hole 23. When the buoyancy ring 34 is moved upward by the buoyancy of the liquid in the test tube 5, the buoyancy ring 34 drives the supporting block 33 to move upward. At this time, the injection head 3 continues to move downward until the supporting block 33 abuts against the spring 24. The buoyancy ring 34 is suitable for driving the supporting block 33 to be inserted into the connecting hole 23 to detect whether the depth of the detection head inserted into the liquid surface reaches a preset value. A spring 24 is arranged in each of the connecting holes 23, and the spring 24 is adapted to abut against the support block 33; wherein, when the injection head 3 is inserted into the liquid surface in the test tube 5, the buoyancy ring 34 moves upward due to the buoyancy of the liquid surface in the test tube 5, and at this time, the injection head 3 continues to move upward, and the buoyancy ring 34 moves upward relative to the injection head 3 until the spring 24 abuts against the support block 33; as the injection head 3 continues to move below the liquid surface in the test tube 5, the buoyancy of the liquid surface on the buoyancy ring 34 is less than the thrust of the spring 24 on the support block 33, and at this time, the buoyancy ring 34 moves downward with the injection head 3. If the liquid surface in the test tube 5 overflows the buoyancy ring 34, the depth of the injection head 3 inserted into the test tube 5 reaches the specified depth, and the piston plate 22 moves downward to inject the test liquid in the cylinder 21 into the test tube 5.

[0042] Some embodiments provide a device for real-time monitoring of cell viability, comprising: The syringe 2 is lifted and arranged above the test tube 5 and is suitable for injecting liquid into the test tube 5; The syringe 2 comprises: a barrel 21, which is arranged above the test tube 5 and is hollow inside; The piston plate 22 is arranged in the cylinder 21 for lifting and lowering, and is slidably sealed with the inner wall of the cylinder 21; The injection head 3 is arranged at the bottom of the cylinder 21, and the lower end thereof protrudes from the bottom wall of the cylinder 21; The piston plate 22 moves away from the injection head 3 to absorb the test liquid into the cylinder 21 under negative pressure; The injection head 3 is pushed by the buoyancy to shrink and move into the cylinder 21 , and the detection liquid flows downward to discharge the bubbles formed by the bottom of the injection head 3 contacting the liquid surface in the test tube 5 .

[0043] Furthermore, the injection head 3 is conical; The bottom wall of the injection head 3 is provided with a plurality of exhaust grooves 31 along the radial direction, and the exhaust grooves 31 are suitable for guiding the bubbles to escape toward the outer wall of the injection head 3 .

[0044] Furthermore, a plurality of slide grooves 32 are provided at equal intervals along the axial direction on the outer wall of the injection head 3, and a support block 33 is slidably provided in each of the slide grooves 32. The support block 33 extends radially along the injection head 3, and the support block 33 protrudes from the outer wall of the cylinder body 21; a buoyancy ring 34 is provided on the outside of the injection head 3, and the inner wall of the buoyancy ring 34 is provided at the outer end of the support block 33, and the buoyancy ring 34 is suitable for detecting whether the depth of the injection head 3 inserted into the liquid surface reaches a preset value.

[0045] Furthermore, a plurality of communication holes 23 are formed on the outer wall of the cylinder 21 at equal intervals along the axial direction, and one communication hole 23 corresponds to one support block 33; A spring 24 is disposed in each of the communication holes 23, and the spring 24 is suitable for abutting against the support block 33; The injection head 3 is inserted into the liquid surface of the test tube 5, and the buoyancy ring 34 is moved upward by the buoyancy of the liquid surface of the test tube 5 until the spring 24 abuts against the support block 33; If the liquid level in the test tube 5 overflows the buoyancy ring 34 , the injection head 3 is inserted into the test tube 5 to a specified depth, and the piston plate 22 moves downward to inject the test liquid in the cylinder 21 into the test tube 5 .

[0046] Some embodiments provide a monitoring method, the monitoring method comprising: After the test tube 5 moves to below the syringe 2, the syringe 2 moves downward until the bottom wall of the injection head 3 fits the liquid surface in the test tube 5; The injection head 3 is retracted toward the syringe 2 during the process of being inserted into the liquid surface to discharge the air bubbles at the lower end of the injection head 3; After the injection head 3 is inserted into the liquid surface to a depth reaching a preset value, the syringe 2 is adapted to inject the detection liquid into the test tube 5 .

[0047] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0048] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, terms such as "first", "second" and other numerical terms do not imply an order or sequence when used in this document unless explicitly indicated above. Therefore, without departing from the teachings of the example embodiments, the first element, component, region, layer or section discussed above may be referred to as a second element, component, region, layer or section.

[0049] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A real-time monitoring device for cell viability, characterized in that: include: A detection device (4) adapted to detect liquid in a test tube (5); An injector (2) which is arranged on a side wall of the detection device (4) and has an injection head (3) arranged on its lower end; After the test tube (5) moves to below the syringe (2), the syringe (2) moves downward until the lower bottom wall of the injection head (3) fits the liquid surface in the test tube (5); The injection head (3) is retracted into the syringe (2) during the process of being inserted into the liquid surface, so as to discharge air bubbles at the lower end of the injection head (3); After the injection head (3) is inserted into the test tube to a depth reaching a preset value of the liquid surface, the syringe (2) is adapted to inject the test liquid into the test tube (5).

2. The real-time monitoring device for cell viability according to claim 1, characterized in that: The syringe (2) comprises: a barrel (21) which is arranged above the test tube (5) and is hollow inside; A piston plate (22) is arranged in a lifting manner in the cylinder (21) and is slidably sealed with the inner wall of the cylinder (21); The injection head (3) is arranged to be lifted and lowered at the bottom of the cylinder (21), and the lower end thereof protrudes from the bottom wall of the cylinder (21); The piston disk (22) moves upward to suck the test liquid into the cylinder (21); During the process of inserting the injection head (3) into the liquid surface, the injection head (3) is pushed by the buoyancy force to shrink into the cylinder (21), and the detection liquid in the cylinder (21) flows downward to discharge bubbles formed by the contact between the bottom of the injection head (3) and the liquid surface in the test tube (5).

3. The real-time monitoring device for cell viability according to claim 2, characterized in that: The injection head (3) is conical; The bottom wall of the injection head (3) is provided with a plurality of exhaust grooves (31) in a radial direction, and the exhaust grooves (31) are suitable for guiding bubbles to escape toward the outer wall of the injection head (3).

4. The real-time monitoring device for cell viability according to claim 3, characterized in that: The outer wall of the injection head (3) is provided with a plurality of slide grooves (32) at equal intervals along the axial direction, and a support block (33) is slidably arranged in each of the slide grooves (32). The support block (33) extends radially along the injection head (3), and the support block (33) protrudes from the outer wall of the cylinder (21).

5. The real-time monitoring device for cell viability according to claim 4, characterized in that: The injection head (3) is sleeved with a buoyancy ring (34) on the outside, and the inner wall of the buoyancy ring (34) is arranged at the outer end of the support block (33); The injection head (3) is inserted into the liquid surface until the liquid surface overflows the buoyancy ring (34), and then it is determined that the depth of the injection head (3) inserted into the liquid surface has reached a preset value.

6. The real-time monitoring device for cell viability according to claim 5, characterized in that: The outer wall of the cylinder (21) is provided with a plurality of communication holes (23) at equal intervals along the axial direction, and one communication hole (23) corresponds to one support block (33); The buoyancy ring (34) is suitable for driving the support block (33) to be inserted into the communication hole (23) to detect whether the depth of the detection head inserted into the liquid surface reaches a preset value.

7. The real-time monitoring device for cell viability according to claim 6, characterized in that: A spring (24) is disposed in each of the communication holes (23), and the spring (24) is suitable for abutting against the support block (33); The injection head (3) is inserted into the liquid surface in the test tube (5), and the buoyancy ring (34) moves upward due to the buoyancy of the liquid surface in the test tube (5) until the spring (24) abuts against the support block (33); If the liquid level in the test tube (5) overflows the buoyancy ring (34), the depth of the injection head (3) inserted into the test tube (5) reaches a specified depth, and the piston plate (22) moves downward to inject the test liquid in the cylinder (21) into the test tube (5).

8. A real-time monitoring device for cell viability, characterized in that: include: A syringe (2) which is arranged to be lifted above the test tube (5) and is suitable for injecting liquid into the test tube (5); The syringe (2) comprises: a barrel (21) which is arranged above the test tube (5) and is hollow inside; A piston plate (22) is arranged in a lifting manner in the cylinder (21) and is slidably sealed with the inner wall of the cylinder (21); An injection head (3) which is arranged at the bottom of the cylinder (21) and has a lower end protruding from the bottom wall of the cylinder (21); The piston disk (22) moves in a direction away from the injection head (3) so as to absorb the test liquid into the cylinder (21) under negative pressure; The injection head (3) is pushed by buoyancy to shrink and move into the cylinder (21), and the detection liquid flows downward to discharge bubbles formed by the bottom of the injection head (3) contacting the liquid surface in the test tube (5).

9. The real-time monitoring device for cell viability according to claim 8, characterized in that: The injection head (3) is conical; The bottom wall of the injection head (3) is provided with a plurality of exhaust grooves (31) in a radial direction, and the exhaust grooves (31) are suitable for guiding bubbles to escape toward the outer wall of the injection head (3).

10. The real-time monitoring device for cell viability according to claim 9, characterized in that: The outer wall of the injection head (3) is provided with a plurality of slide grooves (32) at equal intervals along the axial direction, and a support block (33) is slidably arranged in each of the slide grooves (32). The support block (33) extends radially along the injection head (3), and the support block (33) protrudes from the outer wall of the cylinder (21); a buoyancy ring (34) is sleeved on the outside of the injection head (3), and the inner wall of the buoyancy ring (34) is arranged at the outer end of the support block (33). The buoyancy ring (34) is suitable for detecting whether the depth of the injection head (3) inserted into the liquid surface reaches a preset value.

11. The real-time monitoring device for cell viability according to claim 10, characterized in that: The outer wall of the cylinder (21) is provided with a plurality of communication holes (23) at equal intervals along the axial direction, and one communication hole (23) corresponds to one support block (33); A spring (24) is disposed in each of the communication holes (23), and the spring (24) is suitable for abutting against the support block (33); The injection head (3) is inserted into the liquid surface in the test tube (5), and the buoyancy ring (34) moves upward due to the buoyancy of the liquid surface in the test tube (5) until the spring (24) abuts against the support block (33); If the liquid level in the test tube (5) overflows the buoyancy ring (34), the depth of the injection head (3) inserted into the test tube (5) reaches a specified depth, and the piston plate (22) moves downward to inject the test liquid in the cylinder (21) into the test tube (5).

12. A monitoring method, characterized in that: Using the real-time cell viability monitoring device according to any one of claims 1 to 11, the monitoring method comprises: After the test tube (5) moves to below the syringe (2), the syringe (2) moves downward until the lower wall of the injection head (3) fits the liquid surface in the test tube (5); The injection head (3) is retracted toward the syringe (2) during the process of being inserted into the liquid surface, so as to discharge air bubbles at the lower end of the injection head (3); After the injection head (3) is inserted into the liquid surface to a depth reaching a preset value, the syringe (2) is suitable for injecting the detection liquid into the test tube (5).

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