Infant fertilized egg culture equipment for assisted reproduction test tube

Abstract

The invention provides auxiliary reproduction test tube infant fertilized egg culture equipment which comprises a constant-temperature bottom box and a sealing top box covering the top of the constant-temperature bottom box in a sealing mode, a culture dish cavity is formed in the middle of the top of the constant-temperature bottom box, and a variable culture dish with a variable culture plane is arranged in the culture dish cavity; compared with the prior art, when the fertilized eggs of the test tube baby are cultured, a proper amount of nutrient solution is put into the variable culture dish in a sterile laboratory, the fertilized eggs of the test tube baby are put into the nutrient solution to be cultured, and at the moment, the middle of the culture plane of the variable culture dish is concave downwards to be in a funnel shape; the test tube infant fertilized eggs are always located in the middle of the culture plane, cells start to split along with culture, the culture plane of the variable culture dish is gradually adjusted from a funnel shape to a plane shape along with the increase of the cells, and therefore the situation that the test tube infant fertilized eggs are excessively dispersed after being split is avoided, and stacking of multiple cells can be avoided; the observation condition is influenced.

Description

Technical Field

[0001] The present invention relates to the technical field of tissue, human, animal or plant cell or virus culture devices, and in particular to a fertilized egg culture device for assisted reproductive test-tube babies. Background Art

[0002] With the progress of society and the increase in life pressure, young people are getting married later and later, and the childbearing age is gradually increasing. As the childbearing age increases, women's fertility decreases, resulting in many elderly infertile people. In order to solve the problem of infertility, in vitro fertilization-embryo transfer technology has been gradually promoted and developed in the past 30 years. After more than 30 years of development, this technology has achieved great results. However, there are still some shortcomings in this technology. At present, in vitro fertilization and embryo culture are completed in an incubator filled with carbon dioxide. The incubator needs to maintain a constant temperature and a constant concentration of carbon dioxide to simulate the physiological environment of the uterus. The embryos in the culture medium interact with it and exchange substances to ensure the growth of the fertilized eggs and embryos; However, the existing embryo culture devices or incubators still have some defects and deficiencies. The culture planes of the existing IVF fertilized egg culture devices are all flat, which may indeed cause the cells to be too dispersed after division. The cell dispersion easily leads to the weakening of the interaction between cells, thereby affecting the growth rate of cells, causing changes in cell functions, and affecting the normal development and differentiation of cells. Therefore, an assisted reproductive IVF fertilized egg culture device is proposed. Summary of the invention

[0003] In order to solve at least one of the above-mentioned technical shortcomings, the present invention provides an assisted reproductive in vitro fertilization fertilized egg culture device, including a constant temperature bottom box and a sealed top box sealed on the top of the constant temperature bottom box, a culture dish cavity is opened in the middle of the top of the constant temperature bottom box, and a variable culture dish with a changeable culture plane is provided inside the culture dish cavity.

[0004] Furthermore, the variable culture dish includes a culture dish body arranged inside the culture dish cavity, the internal culture plane of the culture dish body is a flexible plane, the interior of the flexible plane is provided with an elastic vortex channel, the air supply end of the elastic vortex channel is integrally connected with an air guide tube, the other end of the air guide tube is fixedly connected with an air cylinder, and the interior of the air cylinder is sealed and connected with a piston that can be pulled up and down.

[0005] Furthermore, a 128×128 microelectrode array made of gold or platinum is etched on the surface of the flexible plane, the electrode spacing of the microelectrode array is between 100 μm and 1 mm, and the center of the microelectrode array overlaps with the center of the elastic vortex channel.

[0006] Furthermore, a threaded hole is fixedly connected to the top outer wall of the air cylinder, a rotating hole coaxial with the threaded hole is fixedly connected to the top outer wall of the piston, a threaded rod is rotatably connected inside the rotating hole, and the thread of the threaded rod is threadedly connected to the threaded hole.

[0007] Furthermore, the air cylinder is integrally nested inside the sealed top box, and a connector is provided in the middle of the air guide tube.

[0008] Furthermore, a constant temperature electric heating ceramic sheet is provided inside the constant temperature bottom box, and a heat insulation layer surrounding the constant temperature electric heating ceramic sheet and the periphery of the culture dish cavity is provided inside the outer wall of the constant temperature bottom box.

[0009] Furthermore, a hook-shaped tube is provided at the top of the sealed top box and passes through the interior. The top end of the hook-shaped tube is vertically arranged and the bottom end is S-shaped. The top end of the hook-shaped tube is sealed with a medical rubber plug. The bottom end of the hook-shaped tube is connected with a dropper through a hose. The hose is connected to the middle of the dropper. The top end of the dropper is connected to the sealed top box as a whole through medical rubber. A cavity for the movement of the dropper is provided inside the sealed top box.

[0010] Furthermore, a microscope lens for observing the culture status of fertilized eggs is provided in the middle of the top of the sealed top box. Beneficial Effects

[0011] Compared with the prior art, when culturing the fertilized egg of the test tube baby, the present invention puts an appropriate amount of nutrient solution in the inside of the variable culture dish in the sterile laboratory, and then places the fertilized egg of the test tube baby in the nutrient solution for culturing. At this time, the middle part of the culture plane of the variable culture dish is concave in a funnel shape, so that the fertilized egg of the test tube baby is always located in the middle part of the culture plane. As the culture proceeds, the cells begin to divide. As the number of cells increases, the culture plane of the variable culture dish is gradually adjusted from the funnel shape to a flat shape, thereby avoiding the situation that the fertilized egg of the test tube baby is too dispersed after the completion of the division, and can avoid the situation that multiple cells are stacked and the observation is affected. When the culture plane of the variable culture dish is gradually adjusted from a funnel shape to a plane shape, the interior of the elastic vortex channel is filled with gas at this time, and the elastic vortex channel located in the outer circle is closer to the air guide tube. When the air guide tube is filled with gas, the piston inside the gas cylinder is pressed inward, and the elastic vortex channel located in the outer circle will expand in advance, so that the upper surface of the flexible plane supported by the elastic vortex channel is funnel-shaped. As the culture proceeds, the gas inside the gas cylinder is gradually sent into the interior of the elastic vortex channel, so that the upper surface of the flexible plane supported by the elastic vortex channel is gradually transformed from a funnel shape to a nearly flat surface, so that when the fertilized eggs of the test tube baby are cultured, as the cell division proceeds, the cells will not be too dispersed and the cells will not be stacked together to affect the observation.

[0012] At different stages of the fertilized egg, the flexible plane can be adjusted according to the cell division situation, and more electrodes can be added to the monitoring. The setting of the microelectrode array can be adjusted according to the cell characteristics at different developmental stages; in the fertilized egg and cleavage stage, the array scale is smaller, which is suitable for recording the electrical activity of a single cell; in the blastocyst and embryo stage, the array scale is increased to capture the electrophysiological information of more cells; the microelectrode array at each stage must adapt to the cell type, size, distribution and required electrophysiological signal type.

[0013] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings in conjunction with the embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the whole of the present invention.

[0015] Figure 2 It is a cross-sectional view of the overall structure of the present invention.

[0016] Figure 3 It is an axonometric view of the variable culture dish of the present invention.

[0017] Figure 4 It is a schematic structural diagram of the flexible plane of the present invention.

[0018] Figure 5 It is a schematic structural diagram of the elastic vortex channel of the present invention.

[0019] Figure 6 It is a schematic structural diagram of the piston of the present invention.

[0020] Figure 7 Schematic diagram of the structure of the microelectrode array of the present invention.

[0021] exist Figures 1 to 7 , the correspondence between the component names or lines and the figure numbers is: constant temperature bottom box 1, culture dish cavity 101, insulation layer 102, constant temperature electric heating ceramic sheet 103, sealed top box 2, hook-shaped tube 201, medical rubber plug 211, dropper 202, microscope lens 203, variable culture dish 3, culture dish body 301, flexible plane 302, elastic vortex channel 321, air guide tube 303, air cylinder 304, threaded hole 341, piston 305, rotating hole 351, threaded rod 352, microelectrode array 4. DETAILED DESCRIPTION

[0022] Please refer to Figures 1 to 7 ; This embodiment provides a fertilized egg culture device for assisted reproduction test tube baby, referring to Figure 1 and Figure 2, comprising a constant temperature bottom box 1 and a sealed top box 2 sealed and covered on the top of the constant temperature bottom box 1, a culture dish cavity 101 is provided in the middle of the top of the constant temperature bottom box 1, and a variable culture dish 3 with a variable culture plane is provided inside the culture dish cavity 101; In a specific implementation, when culturing the fertilized eggs of test tube babies, in a sterile laboratory, an appropriate amount of nutrient solution is placed inside the variable culture dish 3, and the fertilized eggs of test tube babies are placed in the nutrient solution for culture. At this time, the middle part of the culture plane of the variable culture dish 3 is concave in a funnel shape, so that the fertilized eggs of test tube babies are always located in the middle of the culture plane. As the culture proceeds, the cells begin to divide. As the number of cells increases, the culture plane of the variable culture dish 3 is gradually adjusted from the funnel shape to a plane shape, thereby avoiding the situation where the fertilized eggs of test tube babies are too dispersed after the division is completed, and can avoid the situation where multiple cells are stacked and affect the observation.

[0023] For further reference, Figures 2 to 5 The variable culture dish 3 includes a culture dish body 301 disposed inside the culture dish cavity 101, the internal culture plane of the culture dish body 301 is a flexible plane 302, an elastic vortex channel 321 is disposed inside the flexible plane 302, an air supply end of the elastic vortex channel 321 is integrally connected with an air guide tube 303, the other end of the air guide tube 303 is fixedly connected with an air cylinder 304, and the interior of the air cylinder 304 is sealed and connected with a piston 305 that can be pulled up and down; In a specific implementation, when the culture plane of the variable culture dish 3 is gradually adjusted from a funnel shape to a plane shape, the interior of the elastic vortex channel 321 is filled with gas at this time, and the elastic vortex channel 321 located in the outer ring is closer to the air guide tube 303. When the air guide tube 303 is filled with gas, the piston 305 inside the gas cylinder 304 is pressed inward, and the elastic vortex channel 321 located in the outer ring will expand in advance, so that the upper surface of the flexible plane 302 supported by the elastic vortex channel 321 is funnel-shaped. As the culture proceeds, the gas inside the gas cylinder 304 is gradually sent into the interior of the elastic vortex channel 321, so that the upper surface of the flexible plane 302 supported by the elastic vortex channel 321 is gradually transformed from a funnel shape to a plane (a funnel shape with a slight angle), so that when the fertilized eggs of the test tube baby are cultured, as the cell division proceeds, the cells are not too dispersed and the cells are not stacked together to affect the observation; During production, the flexible plane 302 is a polystyrene film, and the elastic vortex channel 321 is made of elastic silicone; When the culture plane of the variable culture dish 3 is gradually adjusted from a flat shape to a funnel shape, the air guide tube 303 draws out gas, and the piston 305 inside the gas cylinder 304 is pulled out, which is exactly the opposite process.

[0024] For further reference, Figure 7The surface of the flexible plane 302 is etched with a 128×128 microelectrode array 4 made of gold or platinum, the electrode spacing of the microelectrode array 4 is between 100 μm and 1 mm, and the center of the microelectrode array 4 overlaps with the center of the elastic vortex channel 321; In specific implementation, the microelectrode array 4 uses electrodes to capture the electrical signals generated by cells during electrophysiological activities; these signals mainly come from the potential changes of the cell membrane, especially the propagation of action potentials; the electrodes detect and record local electric field changes by contacting cells or cell groups; The electrode spacing of the microelectrode array 4 is between 100 μm and 1 mm, which is more convenient for capturing local electrical activity and ensuring the spatial resolution of the signal (i.e., the rectangular space formed between adjacent electrodes of the microelectrode array 4); The microelectrode array 4 is connected to a signal acquisition system (existing equipment) to obtain the electrophysiological signals of the cells in real time. These signals can be used to analyze the developmental status of the fertilized egg; During the whole process of fertilized egg culture, Fertilized egg stage: At this time, the fertilized egg is a single cell and has not yet divided. The fertilized egg is located in the central depression of the flexible plane 302 (the flexible plane 302 is funnel-shaped at this time). At this time, several electrodes in the central area of ​​the microelectrode array 4 are used to record the electrical activity of a single fertilized egg; Cleavage stage: The cleavage stage refers to the time when the fertilized egg begins to divide and becomes multiple daughter cells (the earliest stage of embryonic development). Cell division and differentiation at this stage are relatively fast, but each cell is usually small in size and has relatively simple electrical activities. At the same time, gas is injected into the elastic vortex channel 321 to make the middle depression of the flexible plane 302 move upward a little (to make the depression flatter). As the number of cells increases, the flexible plane 302 is adjusted to accommodate more cells, and more cells can be added to the monitoring with more electrodes in the central area of ​​the microelectrode array 4 to record the electrical activities of the cells after division. Blastocyst stage: The blastocyst stage is the embryonic development process, and the cells have begun to form a structured blastocyst; at this time, the cells have differentiated into different types. As the number of cells increases, the flexible plane 302 is adjusted to accommodate more cells, and more cells can be added to the monitoring with more electrodes in the central area of ​​the microelectrode array 4 to record the electrical activity of the cells after division; Embryonic stage: During the embryonic development stage, cells begin to further differentiate, tissues and organs are gradually formed, and electrical activities become more complex; as the number of cells increases, the flexible plane 302 is adjusted to accommodate more cells, and more cells can be added to the monitoring with more electrodes in the central area of ​​the microelectrode array 4 to record the electrical activities of the cells after division; At different stages of the fertilized egg, the flexible plane 302 can be adjusted according to the cell division situation, and more electrodes can be added to the monitoring. The setting of the microelectrode array 4 can be adjusted according to the cell characteristics at different developmental stages; during the fertilized egg and cleavage stages, the array scale is smaller and suitable for recording the electrical activity of a single cell; during the blastocyst and embryonic stages, the array scale is increased to capture the electrophysiological information of more cells; the microelectrode array 4 at each stage must adapt to the cell type, size, distribution and required electrophysiological signal type.

[0025] For further reference, Figure 6 The top outer wall of the gas cylinder 304 is fixedly connected with a threaded hole 341, the top outer wall of the piston 305 is fixedly connected with a rotating hole 351 coaxial with the threaded hole 341, and the rotating hole 351 is internally rotatably connected with a threaded rod 352, and the thread of the threaded rod 352 is threadedly connected with the threaded hole 341; In a specific implementation, when the piston 305 inside the gas cylinder 304 is pressed inward, the threaded rod 352 is rotated to make the threaded rod 352 engage with the threaded hole 341, so that the threaded rod 352 drives the piston 305 to be pressed inward.

[0026] Furthermore, the air cylinder 304 is integrally nested inside the sealed top box 2, and a connector is provided in the middle of the air guide tube 303; In the specific implementation, the air cylinder 304 is integrally nested inside the sealed top box 2 to reduce the overall volume. Through the setting of the connector, when the constant temperature bottom box 1 and the sealed top box 2 are covered, the middle part of the air guide tube 303 can be assembled through the connector.

[0027] Furthermore, a constant temperature electric heating ceramic sheet 103 is provided inside the constant temperature bottom box 1, and a heat insulation layer 102 surrounding the constant temperature electric heating ceramic sheet 103 and the periphery of the culture dish cavity 101 is provided inside the outer wall of the constant temperature bottom box 1; In specific implementation, when the constant temperature bottom box 1 is maintaining a constant temperature, the constant temperature electric heating ceramic sheet 103 is heated to maintain a constant temperature. The constant temperature technology of the constant temperature electric heating ceramic sheet 103 adopts the existing technology, and the influence of the external temperature is reduced by setting the heat insulation layer 102.

[0028] For further reference, Figure 2 The top of the sealed top box 2 is provided with a hook-shaped tube 201 that runs through the inside. The top of the hook-shaped tube 201 is vertically arranged, and the bottom is S-shaped. The top of the hook-shaped tube 201 is sealed with a medical rubber plug 211. The bottom of the hook-shaped tube 201 is connected with a dropper 202 through a hose. The hose is connected to the middle of the dropper 202. The top of the dropper 202 is connected to the sealed top box 2 through medical rubber. The inside of the sealed top box 2 is provided with a cavity for the dropper 202 to move. In a specific implementation, by setting the hook-shaped tube 201, the bottom of the hook-shaped tube 201 can form a communicating vessel structure. When the nutrient solution is injected into the inside of the hook-shaped tube 201, the nutrient solution can enter the inside of the dropper 202 from the bottom of the hook-shaped tube 201 through the hose, and then drip the nutrient solution into the culture area through the top of the handheld dropper 202.

[0029] Furthermore, a microscope lens 203 for observing the culture of fertilized eggs is provided in the middle of the top of the sealed top box 2; In specific implementation, the microscope lens 203 is provided to facilitate observation of the culture conditions at any time.

Claims

1. An assisted reproductive test tube baby fertilized egg culture device, comprising a constant temperature bottom box (1) and a sealed top box (2) sealed on the top of the constant temperature bottom box (1), characterized in that: A culture dish cavity (101) is provided in the middle of the top of the constant temperature bottom box (1), and a variable culture dish (3) with a variable culture plane is provided inside the culture dish cavity (101).

2. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 1, characterized in that: The variable culture dish (3) comprises a culture dish body (301) arranged inside a culture dish cavity (101); the internal culture plane of the culture dish body (301) is a flexible plane (302); an elastic vortex channel (321) is arranged inside the flexible plane (302); an air supply end of the elastic vortex channel (321) is integrally connected with an air guide tube (303); the other end of the air guide tube (303) is fixedly connected with an air cylinder (304); and a piston (305) that can be pulled up and down is sealed and connected inside the air cylinder (304).

3. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 2, characterized in that: A 128×128 microelectrode array (4) made of gold or platinum is etched on the surface of the flexible plane (302), the electrode spacing of the microelectrode array (4) is between 100 μm and 1 mm, and the center of the microelectrode array (4) overlaps with the center of the elastic vortex channel (321).

4. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 3, characterized in that: A threaded hole (341) is fixedly connected to the top outer wall of the gas cylinder (304), a rotating hole (351) coaxial with the threaded hole (341) is fixedly connected to the top outer wall of the piston (305), a threaded rod (352) is rotatably connected inside the rotating hole (351), and the thread of the threaded rod (352) is threadedly connected to the threaded hole (341).

5. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 4, characterized in that: The air cylinder (304) is integrally nested inside the sealed top box (2), and a connector is provided in the middle of the air guide tube (303).

6. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 5, characterized in that: A constant temperature electric heating ceramic sheet (103) is provided inside the constant temperature bottom box (1), and a heat insulation layer (102) surrounding the constant temperature electric heating ceramic sheet (103) and the periphery of the culture dish cavity (101) is provided inside the outer wall of the constant temperature bottom box (1).

7. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 6, characterized in that: The top of the sealed top box (2) is provided with a hook-shaped tube (201) penetrating the inside, the top of the hook-shaped tube (201) is arranged vertically, and the bottom is arranged in an S-shaped arc shape, the top of the hook-shaped tube (201) is sealedly connected to a medical rubber plug (211), the bottom of the hook-shaped tube (201) is connected to a dropper (202) via a hose, the hose is connected to the middle of the dropper (202), the top of the dropper (202) is connected to the sealed top box (2) via medical rubber to form an integral body, and a cavity for the movement of the dropper (202) is provided inside the sealed top box (2).

8. The fertilized egg culture device for assisted reproduction in vitro fertilization according to claim 7, characterized in that: A microscope lens (203) for observing the culture status of fertilized eggs is provided in the middle of the top of the sealed top box (2).

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