Preparation method of morchella strain

By optimizing the medium formula and production process of morel strains, including adding nitrogen source, carbohydrates and vitamins to wheat grains, and using an automated bacterial encapsulation bag device, the growth difference and culture in traditional strain production are solved. The problem of excessive cycle has achieved efficient and good quality bacterial strain production.

CN119452996AInactive Publication Date: 2025-02-18HUBEI PIAOYANG FOOD TECH CO LTD
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Patent Information

Application Number
CN202411398530.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-09
Publication Date
2025-02-18
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The traditional morel strain production process has significant differences in the growth of bacterial strains due to irregular raw materials, immature production processes and unclear cultivation parameters. The mycelium culture cycle is too long and its vitality is reduced, which seriously affects the quality of bacterial strains.

Method used

By determining suitable medium formulas, including wheat grains, rice husks, wood chips, soil, calcium carbonate and gypsum, and adding inorganic nitrogen sources, carbohydrates and vitamins to the wheat grains, promote microbial growth and reproduction. Intravenous inoculation method and high-temperature resistant polypropylene plastic bags are used for bacteria packaging, and automatic packaging and heating and sterilization are used for bacterial packaging and sterilization.

Benefits of technology

The mycelium culture cycle is shortened, the activity and yield of bacterial strains are improved, the quality of bacterial strains is improved, and the production efficiency and operation convenience are improved through automated processes.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of morchella culture, and discloses a morchella strain production method, which comprises: S1, determining a culture medium formula: taking 50-60% by mass of wheat grains, 10-25% by mass of rice husk, 10-20% by mass of wood dust, 10-18% by mass of soil, 1% by mass of calcium carbonate, and 1% by mass of gypsum, and the sum of the mass percentages of the materials is 100%, a mixture is mixed in the wheat grains and comprises an inorganic nitrogen source, carbohydrates and vitamins; s2, preparing a culture material; s3, making a fungus bag; s4, inoculating; and S5, culturing. The method has the following advantages and effects that the nitrogen source, the carbohydrate and the vitamin are added into the wheat grains, the carbohydrate provides an energy source for microorganisms, the appropriate nitrogen source and carbon source promote growth and reproduction of the microorganisms, and then the mycelium culture period is shortened.
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Description

Technical Field

[0001] The invention relates to the technical field of Morchella cultivation, and in particular to a method for preparing Morchella spawn. Background Art

[0002] Morchella strains are the most basic means of production in Morchella production, and play a very important role in determining the yield and quality of Morchella. Morchella strains are divided into primary strains (also called mother strains or test tube strains), secondary strains (also called original strains), and tertiary strains (also called cultivated strains or production strains). Usually, mother strains are used to cultivate original strains, which are then inoculated into the culture matrix to grow into cultivated strains for planting. Strain is the first step in mushroom cultivation. The quality of strains determines the vitality and physiological state of the bacteria, thus affecting the yield of the mushrooms (fruiting bodies) finally harvested.

[0003] The traditional strain production process has significant differences in the growth of each batch of strains due to non-standard raw materials, immature production processes, unclear culture parameters and other reasons. The strain culture cycle is too long, and mycelium aging and reduced vitality occur, which seriously affects the strain quality. Summary of the invention

[0004] The purpose of the present invention is to provide a ratchet wrench which has the effect of shortening the mycelium culture period.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions: a method for preparing Morchella spp.

[0006] The following steps are involved:

[0007] S1 determines the culture medium formula: wheat grains are 50%-60%, rice husks are 10%-25%, sawdust is 10-20%, soil is 10%-18%, calcium carbonate is 1%, and gypsum is 1%, and the total weight percentage of the above materials is 100%, wherein the wheat grains are mixed with a mixture, and the mixture is an inorganic nitrogen source, carbohydrates and vitamins;

[0008] S2 prepares culture medium: mixes ingredients according to the culture medium formula determined in S1, adds water and stirs, and controls the moisture content of the culture medium to 40%;

[0009] S3: preparing a mushroom bag: using a high temperature resistant polypropylene plastic bag to package the mushroom strain, using a mushroom bag sealing device (1) to cover the mushroom bag, and then heating and sterilizing the mushroom bag and cooling it;

[0010] S4 inoculation: using the middle inoculation method, inserting the Morchella spawn stick in the middle of the fungus bag to increase air permeability and mycelium growth rate;

[0011] S5 Cultivation: The inoculated bacteria bag is placed in a culture room and cultured at 12°C-20°C for 25-40 days until the culture container is full of mycelium for the next level of inoculation or sowing.

[0012] By adopting the above technical scheme, nitrogen sources, carbohydrates and vitamins are added to wheat grains. Carbohydrates provide an energy source for microorganisms, and suitable nitrogen sources and carbon sources promote the growth and reproduction of microorganisms. During the fermentation process, sufficient nutrients accelerate metabolic reactions and increase product formation, thereby shortening the mycelium culture cycle and improving culture efficiency.

[0013] The present invention is further configured as follows: the inorganic nitrogen source is potassium nitrate.

[0014] By adopting the above technical solution, an available nitrogen source is provided for morels to promote their growth and reproduction. Potassium nitrate releases hydrogen ions when dissolved, which has a certain effect on the pH value of the culture medium and helps to maintain a suitable growth environment.

[0015] The present invention is further configured as follows: the wheat grains provide a carbon source, the potassium nitrate provides a nitrogen source, and the ratio of the carbon source to the nitrogen source is 20:1 in terms of mass percentage.

[0016] By adopting the above technical solution, the growth of mycelium is promoted and the activity and yield of the fungus are improved.

[0017] The present invention is further configured as follows: the mushroom bag sealing device (1) comprises a conveying platform for conveying mushroom bags, a conveyor belt driven on the conveying platform and a material discharge chute fixed on one side of the conveying platform, a material transfer platform is arranged on one side of the conveying platform, a material transfer plate is rotatably connected to the material transfer platform, a slot is arranged on the material transfer plate, an end of the material discharge chute is located above the slot, the material transfer platform is equipped with a driving device for driving the material transfer plate to rotate, and also comprises a bag twisting mechanism, a ring mechanism and a capping mechanism which are arranged in sequence along the rotation direction of the material transfer plate, the bag twisting mechanism is used to rotate and close the mouth of the mushroom bag, the ring mechanism is used to connect the ring to the mouth of the mushroom bag, and the capping mechanism is used to connect the cover body to the top of the ring to complete the mushroom bag packaging.

[0018] By adopting the above technical scheme, the mushroom bag filled with culture medium is placed on the conveyor belt for transportation, and the upper opening of the mushroom bag falls into the notch on the transfer plate through the discharge chute. The driving device drives the transfer plate to rotate, and then drives the mushroom bag to rotate around the transfer platform and pass through the bag twisting mechanism, the ring mechanism and the capping mechanism in sequence. The bag twisting mechanism twists and rotates the mouth of the mushroom bag to make the mouth of the mushroom bag in a retracted state, the ring mechanism puts the ring on the mouth of the mushroom bag, and then the capping mechanism drives the cover body to be connected to the ring to complete the automatic packaging of the mushroom bag, thereby realizing the multi-quantity culture of morel mushroom species, improving the culture efficiency, facilitating operation and saving manpower.

[0019] The present invention is further configured as follows: the driving device includes a rotating shaft fixed to the bottom of the material transfer plate and rotating on the material transfer platform, a first gear fixed to the rotating shaft and a driving motor fixed to the bottom of the material transfer platform, the output end of the driving motor is fixed with a second gear meshed with the first gear, both ends of the conveying platform are rotatably connected with transmission rollers, the conveyor belt is sleeved between the two transmission rollers, both ends of the transmission roller are fixed with roller shafts, the bottom end of the rotating shaft is fixed with a first bevel gear, the bottom end of the material transfer platform is rotatably connected with a transmission shaft, the transmission shaft is fixed with a second bevel gear meshed with the first bevel gear, and the transmission shaft and the roller shaft are connected by a transmission belt.

[0020] By adopting the above technical solution, the driving motor drives the second gear to rotate and then drives the first gear and the rotating shaft to rotate, driving the material transfer plate to rotate around the material transfer platform to transport the mushroom bag. During this period, when the material transfer plate and the rotating shaft rotate, the first bevel gear, the second bevel gear and the transmission shaft rotate synchronously, and the roller shaft and the transmission roller are driven to rotate by the transmission belt, so that when a mushroom bag enters the next step of the process, the conveyor belt transports the next mushroom bag so that it passes through the discharge chute and falls into the slot, thereby realizing continuous sealing of multiple mushroom bags and improving packaging efficiency.

[0021] The present invention is further configured as follows: the notches are evenly distributed along the circumference of the material transfer plate, and a discharge port corresponding to one of the notches is provided at the bottom of the material transfer platform.

[0022] By adopting the above technical solution, when the material transfer plate drives the mushroom bag through the bag twisting mechanism, the ring mechanism and the capping mechanism, after the mushroom bag is sealed, the material transfer plate drives the mushroom bag to continue to rotate around the material transfer table until the discharge port corresponds to the groove, and the mushroom bag falls out of the discharge port to realize automatic unloading.

[0023] The present invention is further configured as follows: the bag-twisting mechanism includes a bag-twisting frame, a bag-twisting shaft rotating on the bag-twisting frame, a third gear and a clamping assembly respectively fixed to the upper and lower ends of the bag-twisting shaft, a bag-twisting motor installed on the bag-twisting frame, and a fourth gear fixed to the output end of the bag-twisting motor and meshing with the third gear; the material transfer platform is fixed with a fixing frame, the material transfer plate is provided with a plug hole leading to the slot, a limiting rod is inserted and slidably on the fixing frame, the rear end of the limiting rod passes through the plug hole and extends into the slot, the end of the limiting rod is configured as an arc shape, and a limiting spring is fixed between one end of the limiting rod away from the slot and the fixing frame.

[0024] By adopting the above technical scheme, the mushroom bag falls into the notch on the material transfer plate through the material discharge chute. When the material transfer plate drives the mushroom bag to move close to the bag twisting mechanism, the limit rod extends into the notch after the limit spring pushes the lower end through the insertion hole and abuts against the bag body of the mushroom bag to clamp the bag body of the mushroom bag and restrict its movement. The clamping assembly clamps the bag mouth of the mushroom bag, and the bag twisting motor drives the third gear and the bag twisting shaft to rotate through the fourth gear, thereby driving the clamping assembly and the clamped bag mouth of the mushroom bag to rotate, so as to achieve the closing of the bag mouth of the mushroom bag, which is convenient for subsequent ring and capping work; then the clamping assembly releases the bag mouth of the mushroom bag, and the material transfer plate continues to rotate to drive the mushroom bag forward. The inner wall of the insertion hole pushes the end of the limit rod to extend out of the notch, and the limit spring contracts, so that during the rotation of the material transfer plate, the end of the limit rod abuts against the outer wall of the material transfer plate.

[0025] The present invention is further configured as follows: the ring mechanism includes a ring frame, a material guide trough fixed on the ring frame and a ring cylinder, a second group of the clamping components is fixed to the output end of the ring cylinder, the capping mechanism includes a capping frame, a second material guide trough fixed to the capping frame, a material storage rack close to the capping frame, a material storage table sliding on the material storage rack, a lifting cylinder close to the material storage rack, a capping cylinder fixed to the output end of the lifting cylinder and a third group of the clamping components fixed to the output end of the capping cylinder, a sliding rod inserted and slidable on the material storage rack is fixed to the bottom of the storage table, a telescopic spring sleeved on the outside of the sliding rod is fixed between the storage table and the storage rack, and a material discharge port and a control component for controlling the switch of the material discharge port are provided at the bottom of the material guide trough.

[0026] By adopting the above technical scheme, the rings are placed side by side on the material guide trough, and the cover body is placed side by side on another material guide trough. The control component controls the feed opening switch, and the feed opening of the material guide trough with the ring is located directly above the trough opening; when the material transfer plate drives the mushroom bag to move close to the ring mechanism, the clamping component first clamps the mushroom bag opening to a limit position, and the control component controls the feed opening to open, and the ring falls from the material guide trough to be sleeved on the bag opening of the mushroom bag, and the clamping component limits the position of the ring. Then the control component controls the feed opening of another material guide trough to open, and the cover body falls from the material guide trough to the material placement table, and the clamping component clamps the cover body on the material placement table. The output end of the covering cylinder extends to drive the clamping component and the cover body to move above the mushroom bag. When the cover body moves to above the ring on the mushroom bag, the output end of the lifting cylinder returns to drive the cover body to be pressed down, and finally the cover body is covered above the ring to achieve the sealing work of the mushroom bag.

[0027] The present invention is further configured as follows: the control component includes two baffles that slide in mirror image at the discharge port, a control motor fixedly mounted on the material guide trough and a control gear fixed to the output end of the control motor, racks located on both sides of the control gear are fixed on the same side of the two baffles, a T-shaped bar is fixed at the bottom of the material guide trough, and the baffle is provided with a T-shaped slot that slides on the T-shaped bar.

[0028] By adopting the above technical solution, the control motor drives the control gear to rotate, and then the control gear drives the racks on both sides to move in opposite directions, and then the racks on both sides respectively drive the baffles on both sides to move in opposite directions, thereby opening or closing the discharge port. When the baffle moves, the T-bar slides in the T-slot to achieve limiting guidance, making the control process more stable.

[0029] The present invention is further configured as follows: the clamping assembly includes a frame, a screw rod rotatably connected to the frame, a guide rod fixed to the frame and parallel to the screw rod, and a clamping motor driving the screw rod to rotate, both ends of the screw rod are provided with bidirectional threads, and both ends of the screw rod are threadedly connected to a clamping plate that slides on the guide rod.

[0030] By adopting the above technical solution, during the clamping operation, the clamping motor drives the screw rod to rotate, the guide rod is restricted, and the clamping plates at both ends are driven to move relative to each other to clamp the mushroom bag.

[0031] The beneficial effects of the present invention are:

[0032] 1. Add nitrogen source, carbohydrates and vitamins to wheat grains. Carbohydrates provide energy sources for microorganisms. Appropriate nitrogen source and carbon source promote the growth and reproduction of microorganisms. During the fermentation process, sufficient nutrients accelerate metabolic reactions and increase product generation, thereby shortening the mycelium culture cycle and improving the culture efficiency.

[0033] 2. Use a mushroom bag sealing device to seal the mushroom bag, place the mushroom bag filled with culture medium on the conveyor belt for transportation, and the upper opening of the mushroom bag falls into the notch on the transfer plate through the discharge chute. The driving device drives the transfer plate to rotate, and then drives the mushroom bag to rotate around the transfer table and pass through the bag twisting mechanism, the ring mechanism and the capping mechanism in sequence. The bag twisting mechanism twists and rotates the mushroom bag mouth to make the mushroom bag mouth in a retracted state, the ring mechanism puts the ring on the mushroom bag mouth, and then the capping mechanism drives the cover body to be connected to the ring to complete the automatic sealing of the mushroom bag, thereby realizing the multi-quantity culture of morel strains, improving the culture efficiency, facilitating operation and saving manpower. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0035] Figure 1 It is a schematic diagram of the structure of the present invention.

[0036] Figure 2 This is a schematic diagram of the bottom structure of the present invention

[0037] Figure 3 yes Figure 2 Enlarged view of point A in the middle.

[0038] Figure 4 yes Figure 2 Enlarged view of point B in the middle.

[0039] In the figure, 1, a bag sealing device for bacteria packaging; 2, a conveying platform; 3, a conveying belt; 4, a feeding trough; 5, a feeding platform; 6, a feeding plate; 7, a driving device; 71, a rotating shaft; 72, a first gear; 73, a driving motor; 74, a second gear; 75, a first bevel gear; 76, a transmission shaft; 77, a second bevel gear; 78, a transmission belt; 8, a bag twisting mechanism; 81, a bag twisting rack; 82, a bag twisting shaft; 83, a third gear; 84, a fourth gear; 9, a collar mechanism; 91, a collar rack; 92, a collar cylinder; 10, a capping mechanism; 101, a capping rack; 102, a material placement rack; 1 03, loading platform; 104, lifting cylinder; 105, covering cylinder; 106, sliding rod; 107, telescopic spring; 11, roller; 12, unloading port; 13, clamping assembly; 131, frame; 132, screw rod; 133, guide rod; 134, clamping motor; 135, clamping plate; 14, fixing frame; 15, socket; 16, notch; 17, limit rod; 18, limit spring; 19, material guide trough; 20, unloading port; 21, control assembly; 211, baffle; 212, control motor; 213, control gear; 214, rack; 215, T-bar. DETAILED DESCRIPTION

[0040] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Embodiment 1, a method for preparing Morchella spp., comprising the following steps:

[0042] S1 determines the culture medium formula: wheat grains are 50%-60%, rice husks are 10%-25%, sawdust is 10-20%, soil is 10%-18%, calcium carbonate is 1%, and gypsum is 1%, and the total weight percentage of the above materials is 100%, wherein the wheat grains are mixed with a mixture, and the mixture is an inorganic nitrogen source, carbohydrates and vitamins;

[0043] S2 prepares culture medium: mixes ingredients according to the culture medium formula determined in S1, adds water and stirs, and controls the moisture content of the culture medium to 40%;

[0044] S3: preparing a mushroom bag: using a high temperature resistant polypropylene plastic bag to package the mushroom strain, using a mushroom bag sealing device (1) to cover the mushroom bag, and then heating and sterilizing the mushroom bag and cooling it;

[0045] S4 inoculation: using the middle inoculation method, inserting the Morchella spawn stick in the middle of the fungus bag to increase air permeability and mycelium growth rate;

[0046] S5 Cultivation: The inoculated bacteria bag is placed in a culture room and cultured at 12°C-20°C for 25-40 days until the culture container is full of mycelium for the next level of inoculation or sowing.

[0047] Furthermore, the inorganic nitrogen source is potassium nitrate.

[0048] Furthermore, the wheat grains provide a carbon source, and the potassium nitrate provides a nitrogen source, and the ratio of the carbon source to the nitrogen source is 20:1 in mass percentage.

[0049] Further, such as Figure 1 As shown, the mushroom bag sealing device 1 includes a conveying platform 2 for conveying mushroom bags, a conveyor belt 3 driven on the conveying platform 2 and a material discharge chute 4 fixed on one side of the conveying platform 2, a material transfer platform 5 is arranged on one side of the conveying platform 2, a material transfer plate 6 is rotatably connected to the material transfer platform 5, a notch 16 is arranged on the material transfer plate 6, an end of the material discharge chute 4 is located above the notch 16, the material transfer platform 5 is equipped with a driving device 7 for driving the material transfer plate 6 to rotate, and also includes a bag twisting mechanism 8, a ring mechanism 9 and a capping mechanism 10 which are sequentially arranged along the rotation direction of the material transfer plate 6, the bag twisting mechanism 8 is used to rotate and close the mouth of the mushroom bag, the ring mechanism 9 is used to sleeve the ring on the mouth of the mushroom bag, and the capping mechanism 10 is used to connect the cover body to the top of the ring to complete the mushroom bag packaging.

[0050] Further, such as Figure 1-Figure 3 As shown, the driving device 7 includes a rotating shaft 71 fixed to the bottom of the material transfer plate 6 and rotating on the material transfer platform 5, a first gear 72 fixed to the rotating shaft 71 and a driving motor 73 fixed to the bottom of the material transfer platform 5, and a second gear 74 meshing with the first gear 72 is fixed to the output end of the driving motor 73. Transmission rollers are rotatably connected at both ends of the conveying platform 2, and the conveyor belt 3 is sleeved between the two transmission rollers. Roller shafts 11 are fixed at both ends of the transmission rollers, and a first bevel gear 75 is fixed to the bottom end of the rotating shaft 71. A transmission shaft 76 is rotatably connected to the bottom end of the material transfer platform 5, and a second bevel gear 77 meshing with the first bevel gear 75 is fixed to the transmission shaft 76. The transmission shaft 76 and the roller shaft 11 are connected through a transmission belt 78.

[0051] Further, such as Figure 1 As shown, the slots 16 are evenly distributed along the circumference of the material transfer plate 6 , and a discharge port 12 corresponding to one of the slots 16 is opened at the bottom of the material transfer platform 5 .

[0052] Further, such as Figure 1, Figure 2 As shown, the bag twisting mechanism 8 includes a bag twisting frame 81, a bag twisting shaft 82 rotating on the bag twisting frame 81, a third gear 83 and a clamping assembly 13 respectively fixed to the upper and lower ends of the bag twisting shaft 82, a bag twisting motor installed on the bag twisting frame 81 and a fourth gear 84 fixed to the output end of the bag twisting motor and meshing with the third gear 83, the material transfer platform 5 is fixed with a fixing frame 14, the material transfer plate 6 is provided with a plug hole 15 leading to the slot 16, and a limiting rod 17 is inserted and slidably on the fixing frame 14, the rear end of which passes through the plug hole 15 and extends into the slot 16, the end of the limiting rod 17 is set to be arc-shaped, and a limiting spring 18 is fixed between the end of the limiting rod 17 away from the slot 16 and the fixing frame 14.

[0053] Further, such as Figure 1 , Figure 2 As shown, the collar mechanism 9 includes a collar frame 91, a guide trough 19 fixed on the collar frame 91, and a collar cylinder 92, and a second set of clamping components 13 are fixed at the output end of the collar cylinder 92. The capping mechanism 10 includes a capping frame 101, a second guide trough 19 fixed to the capping frame 101, a material rack 102 close to the capping frame 101, a material rack 103 sliding on the material rack 102, and a lifting cylinder 104 close to the material rack 102. , a covering cylinder 105 fixed to the output end of the lifting cylinder 104 and a third group of clamping components 13 fixed to the output end of the covering cylinder 105, a sliding rod 106 inserted and slid on the material rack 102 is fixed at the bottom of the material placement table 103, a telescopic spring 107 sleeved on the outside of the sliding rod 106 is fixed between the material placement table 103 and the material rack 102, and a material discharge port 20 and a control component 21 for controlling the switch of the material discharge port 20 are provided at the bottom of the material guide trough 19.

[0054] Further, such as Figure 4 As shown, the control component 21 includes two baffles 211 that slide in mirror image at the discharge port 20, a control motor 212 fixedly mounted on the guide trough 19, and a control gear 213 fixed to the output end of the control motor 212. Racks 214 located on both sides of the control gear 213 are fixed on the same side of the two baffles 211, a T-bar 215 is fixed at the bottom of the guide trough 19, and the baffle 211 is provided with a T-slot that slides on the T-bar 215.

[0055] Further, such as Figure 4 As shown, the clamping assembly 13 includes a frame 131, a screw rod 132 rotatably connected to the frame 131, a guide rod 133 fixed to the frame 131 and parallel to the screw rod 132, and a clamping motor 134 driving the screw rod 132 to rotate. Both ends of the screw rod 132 are set as bidirectional threads, and both ends of the screw rod 132 are threadedly connected to a clamping plate 135 that slides on the guide rod 133.

[0056] Working principle: The bacteria bag filled with culture medium is placed on the conveyor belt 3 for transportation. The upper opening of the bacteria bag falls into the slot 16 on the transfer plate 6 through the feed chute 4. The driving motor 73 drives the second gear 74 to rotate and then drives the first gear 72 and the rotating shaft 71 to rotate, driving the transfer plate 6 to rotate around the transfer platform 5 to transport the bacteria bag. During this period, when the transfer plate 6 and the rotating shaft 71 rotate, the first bevel gear 75, the second bevel gear 77 and the transmission shaft 76 rotate synchronously, and the roller 11 and the transmission roller are driven to rotate through the transmission belt 78, so that when a bacteria bag enters the next step, the conveyor belt 3 transports the next bacteria bag so that it passes through the feed chute 4 and falls into the slot 16, realizing the continuous transfer of multiple bacteria bags. When the bag is sealed and the material transfer plate 6 drives the mushroom bag to move close to the bag twisting mechanism 8, the limit rod 17 extends into the slot 16 after the limit spring 18 pushes the lower end through the insertion hole 15 and clamps the bag body of the mushroom bag to restrict its movement. The clamping motor 134 drives the screw rod 132 to rotate, and the guide rod 133 realizes the restriction, driving the clamping plates 135 at both ends to move relative to clamp the bag mouth of the mushroom bag. The bag twisting motor drives the third gear 83 and the bag twisting shaft 82 to rotate through the fourth gear 84, and then drives the clamping assembly 13 and the clamped bag mouth of the mushroom bag to rotate, so as to realize the closing of the bag mouth of the mushroom bag, which is convenient for the subsequent ring and capping work; then the clamping assembly 13 releases the bag mouth of the mushroom bag, and the material transfer plate 6 continues to The continuous rotation drives the mushroom bag to move forward, and the inner wall of the socket 15 pushes the end of the limit rod 17 to extend out of the slot 16, and the limit spring 18 contracts, so that during the rotation of the feed plate 6, the end of the limit rod 17 abuts against the outer wall of the feed plate 6; the feed plate 6 drives the mushroom bag to continue to move forward. When the feed plate 6 drives the mushroom bag to move close to the collar mechanism 9, the clamping assembly 13 first clamps the mouth of the mushroom bag to limit the position, and the control motor 212 drives the control gear 213 to rotate, and then the control gear 213 drives the racks 214 on both sides to move in opposite directions, and then the racks 214 on both sides respectively drive the baffles 211 on both sides to move in opposite directions, so as to realize the opening of the discharge port 20, and the collar is removed from the guide groove 19. The cover body is placed on the material platform 103, and the clamping assembly 13 clamps the cover body on the material platform 103. The output end of the capping cylinder 105 extends to drive the clamping assembly 13 and the cover body to move above the mushroom bag. When the cover body moves to the position above the collar on the mushroom bag, the mouth of the mushroom bag is pushed by the cover body to be folded. Then the output end of the lifting cylinder 104 returns to drive the cover body to be pressed down. Finally, the cover body is capped above the collar, and the mouth of the mushroom bag is pressed between the cover body and the collar to achieve the sealing of the mushroom bag.

[0057] The embodiments described above are merely preferred embodiments of the present application and are not intended to limit the protection scope of the present application. Therefore, any equivalent changes made by ordinary technicians in this field based on the structure, shape, and principle of the present application without making any creative work should be included in the protection scope of the present application.

Claims

1. A method for preparing Morchella spp., characterized in that: The following steps are involved: S1 determines the culture medium formula: wheat grains are 50%-60%, rice husks are 10%-25%, sawdust is 10-20%, soil is 10%-18%, calcium carbonate is 1%, and gypsum is 1%, and the total weight percentage of the above materials is 100%, wherein the wheat grains are mixed with a mixture, and the mixture is an inorganic nitrogen source, carbohydrates and vitamins; S2 prepares culture medium: mixes ingredients according to the culture medium formula determined in S1, adds water and stirs, and controls the moisture content of the culture medium to 40%; S3: preparing a mushroom bag: using a high temperature resistant polypropylene plastic bag to package the mushroom strain, using a mushroom bag sealing device (1) to cover the mushroom bag, and then heating and sterilizing the mushroom bag and cooling it; S4 inoculation: using the middle inoculation method, inserting the Morchella spawn stick in the middle of the fungus bag to increase air permeability and mycelium growth rate; S5 Cultivation: The inoculated bacteria bag is placed in a culture room and cultured at 12°C-20°C for 25-40 days until the culture container is full of mycelium for the next level of inoculation or sowing.

2. The method for producing a Morchella spawn according to claim 1, characterized in that: The inorganic nitrogen source is potassium nitrate.

3. The method for producing a Morchella spore species according to claim 2, characterized in that: The wheat grains provide a carbon source, and the potassium nitrate provides a nitrogen source. In terms of mass percentage, the ratio of the carbon source to the nitrogen source is 20:

1.

4. The method for producing a Morchella spawn according to claim 1, characterized in that: The mushroom bag sealing device (1) comprises a conveying platform (2) for conveying mushroom bags, a conveying belt (3) driven on the conveying platform (2) and a material discharge chute (4) fixed on one side of the conveying platform (2); a material transfer platform (5) is arranged on one side of the conveying platform (2); a material transfer plate (6) is rotatably connected to the material transfer platform (5); a notch (16) is arranged on the material transfer plate (6); an end of the material discharge chute (4) is located above the notch (16); the material transfer platform (5) is provided with a driving device (7) for driving the material transfer plate (6) to rotate; and further comprises a bag twisting mechanism (8), a ring mechanism (9) and a capping mechanism (10) which are arranged in sequence along the rotation direction of the material transfer plate (6); the bag twisting mechanism (8) is used to rotate and seal the mouth of the mushroom bag; the ring mechanism (9) is used to sleeve the ring on the mouth of the mushroom bag; and the capping mechanism (10) is used to connect the cover body above the ring to complete the mushroom bag packaging.

5. The method for producing a Morchella spawn according to claim 4, characterized in that: The driving device (7) comprises a rotating shaft (71) fixed to the bottom of the material transfer plate (6) and rotating on the material transfer platform (5), a first gear (72) fixed to the rotating shaft (71) and a driving motor (73) fixed to the bottom of the material transfer platform (5); a second gear (74) meshing with the first gear (72) is fixed to the output end of the driving motor (73); transmission rollers are rotatably connected at both ends of the conveying platform (2); the conveying belt (3) is sleeved between the two transmission rollers; roller shafts (11) are fixed at both ends of the transmission rollers; a first bevel gear (75) is fixed to the bottom end of the rotating shaft (71); a transmission shaft (76) is rotatably connected to the bottom end of the material transfer platform (5); a second bevel gear (77) meshing with the first bevel gear (75) is fixed to the transmission shaft (76); and the transmission shaft (76) and the roller shaft (11) are connected in transmission via a transmission belt (78).

6. The method for producing a Morchella spawn according to claim 4, characterized in that: The notches (16) are evenly distributed along the circumference of the material transfer plate (6), and a discharge port (12) corresponding to one of the notches (16) is provided at the bottom of the material transfer platform (5).

7. The method for producing a Morchella spawn according to claim 4, characterized in that: The bag twisting mechanism (8) comprises a bag twisting frame (81), a bag twisting shaft (82) rotating on the bag twisting frame (81), a third gear (83) and a clamping assembly (13) respectively fixed to the upper and lower ends of the bag twisting shaft (82), a bag twisting motor installed on the bag twisting frame (81), and a fourth gear (84) fixed to the output end of the bag twisting motor and meshing with the third gear (83). The material conveying platform (5) is fixed with a fixing frame (14), the material conveying plate (6) is provided with a plug hole (15) leading to the slot (16), a limiting rod (17) is inserted and slidably connected on the fixing frame (14), and the rear end of the limiting rod (17) passes through the plug hole (15) and extends into the slot (16), the end of the limiting rod (17) is set to be arc-shaped, and a limiting spring (18) is fixed between the end of the limiting rod (17) away from the slot (16) and the fixing frame (14).

8. The method for producing a Morchella spawn according to claim 7, characterized in that: The collar mechanism (9) comprises a collar frame (91), a material guide trough (19) fixed on the collar frame (91) and a collar cylinder (92); a second group of the clamping components (13) are fixed on the output end of the collar cylinder (92); the capping mechanism (10) comprises a capping frame (101), a second material guide trough (19) fixed on the capping frame (101), a material placement frame (102) close to the capping frame (101), a material placement table (103) sliding on the material placement frame (102), and a lifting cylinder (104) close to the material placement frame (102). , a covering cylinder (105) fixed to the output end of the lifting cylinder (104) and a third group of the clamping components (13) fixed to the output end of the covering cylinder (105); a sliding rod (106) inserted and slidably connected to the material rack (102) is fixed at the bottom of the material placement platform (103); a telescopic spring (107) sleeved on the outside of the sliding rod (106) is fixed between the material placement platform (103) and the material rack (102); and a material discharge port (20) and a control component (21) for controlling the switch of the material discharge port (20) are provided at the bottom of the material guide trough (19).

9. The method for producing Morchella spp. according to claim 8, characterized in that: The control assembly (21) comprises two baffles (211) which slide in mirror image at the material discharge port (20), a control motor (212) fixedly mounted on the material guide trough (19), and a control gear (213) fixed to the output end of the control motor (212); racks (214) respectively located on both sides of the control gear (213) are fixed on the same side of the two baffles (211); a T-shaped bar (215) is fixed at the bottom of the material guide trough (19); and the baffle (211) is provided with a T-shaped slot which slides on the T-shaped bar (215).

10. The method for producing Morchella spp. according to claim 9, characterized in that: The clamping assembly (13) comprises a frame (131), a screw rod (132) rotatably connected to the frame (131), a guide rod (133) fixed to the frame (131) and parallel to the screw rod (132), and a clamping motor (134) for driving the screw rod (132) to rotate, wherein both ends of the screw rod (132) are provided with bidirectional threads, and both ends of the screw rod (132) are threadedly connected to a clamping plate (135) that slides on the guide rod (133).