Plant growth regulator containing biological enzyme

Abstract

The invention belongs to the technical field of agriculture, and particularly relates to a biological enzyme-containing plant growth regulator which comprises the following components in percentage by weight: 0.05%-5% of an active component A, 0.1%-10% of an active component B, 0.5%-3% of a biological enzyme, 1%-30% of a surfactant, 1%-10% of a solubilizer and the balance of deionized water. The plant growth regulator provided by the invention can promote crop growth and improve crop adaptability, is safe to crops, facilitates better growth, flowering and fruiting of crops, and achieves the effects of increasing yield and improving quality.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and in particular relates to a plant growth regulator containing a biological enzyme and a preparation method thereof. Background Art

[0002] Plant growth regulators, also known as plant exogenous hormones (plant growth regulators, PGRs), are synthetic compounds with physiological activity and similar to plant hormones. Plant growth regulators are compounds or biological substances that can affect plant growth and development. They play an important role in modern agricultural production and are of great significance to increasing crop yields, improving crop quality, and achieving efficient planting.

[0003] Diethyl aminoethyl hexanoate is a synthetic broad-spectrum multi-purpose plant growth regulator. It has the function of cell division, accelerates plant carbon and nitrogen metabolism, increases the content of antioxidant enzymes, increases the content of auxin, and promotes cell division. In addition, it can also increase the content of chlorophyll, protein, sugar, and vitamins in plants, strengthen plant physiological activities, and promote plant photosynthetic rate. At the same time, it can also allocate nutrients, balance the ratio of endogenous hormones, enhance cold resistance and drought resistance, improve plant adaptability, facilitate better growth, flowering, and fruiting of plants, and achieve the effect of increasing production and quality.

[0004] Sodium nitrophenolate is a powerful cell activator, the main chemical components of which are sodium 5-nitroguaiacol, sodium o-nitrophenolate and sodium p-nitrophenolate. Sodium nitrophenolate is a powerful cell activator. Low concentrations of sodium nitrophenolate can quickly penetrate into the plant body after contacting with the plant, promote the flow of cell protoplasm and improve cell vitality.

[0005] Plant growth regulators can regulate plant growth and have the characteristics of wide application, small dosage, fast speed, high efficiency and low residue. Research on plant growth regulators is not only crucial to improving agricultural productivity, but also has a positive impact on sustainable agricultural practices and environmental care. Understanding their mechanism of action and impact on different crops is crucial to promoting the development of agricultural productivity and meeting the world's growing food demand. Summary of the invention

[0006] The purpose of the present invention is to provide a plant growth regulator containing biological enzymes and a preparation method thereof. By compounding sodium nitrophenolate, ammonium ethyl hexanoate, biological enzyme preparations and auxiliary agents, the growth regulation of crops can be strengthened, the growth of crops can be promoted, the adaptability of crops can be improved, the safety of crops can be achieved, and better growth, flowering and fruiting of crops can be facilitated, so as to achieve the effect of increasing production and quality.

[0007] To achieve the above object, the technical solution adopted by the present invention is: a plant growth regulator containing biological enzymes, the plant growth regulator is composed of the following components in weight percentage: active ingredient A 0.05% to 5%, active ingredient B 0.1% to 10%, biological enzyme preparation 0.5% to 10%, surfactant 1% to 30%, solubilizer 1% to 10%, and deionized water supplemented to 100%;

[0008] Furthermore, the active ingredient A is sodium nitrophenolate, and the active ingredient B is ammonium ethyl hexanoate;

[0009] Furthermore, the plant growth regulator is composed of the following components in weight percentage: 0.05% to 5% sodium nitrophenolate, 0.1% to 10% ammonium ethyl hexanoate, 0.5% to 10% biological enzyme preparation, 1% to 30% surfactant, 1% to 10% solubilizer, and deionized water to 100%;

[0010] Furthermore, the plant growth regulator is composed of the following components in weight percentage: 0.1% to 1% sodium nitrophenolate, 1% to 5% diethylaminoethyl hexanoate, 1% to 5% biological enzyme preparation, 5% to 20% surfactant, 2% to 6% solubilizer, and deionized water to 100%;

[0011] Furthermore, the plant growth regulator is composed of the following components in weight percentage: 0.1% to 1% sodium nitrophenolate, 1% to 5% ammonium ethyl hexanoate, 1% to 5% biological enzyme preparation, 15% surfactant, 5% solubilizer, and deionized water to 100%;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 10:1 to 1:10;

[0013] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 5:1 to 1:5 or any ratio between the above values;

[0014] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5;

[0015] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:4;

[0016] Furthermore, the biological enzyme is at least one or more of superoxide dismutase, saccharifying enzyme, protease, amylase, urease and cellulase;

[0017] Furthermore, the biological enzyme is a mixture of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase;

[0018] Furthermore, the mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 2:1:1:1:1;

[0019] Furthermore, the mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 6:1:1:2:2;

[0020] Furthermore, the mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 6:2:2:1:1;

[0021] Furthermore, the surfactant of the plant growth regulator is fatty amine polyoxyethylene ether, fatty amine polyoxyethylene ether quaternary ammonium salt, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, isomeric alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, dodecyl alcohol polyoxyethylene ether, alkyl succinamide sulfonate, fatty alcohol polyoxyethylene ether sulfate, C8-C 10 One or more of alkyl polyglycosides;

[0022] Furthermore, the surfactant of the plant growth regulator is fatty alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, dodecynyl alcohol polyoxyethylene ether;

[0023] Furthermore, the surfactant of the plant growth regulator is fatty alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, and dodecynyl alcohol polyoxyethylene ether in a mass ratio of 3:1:2 and 1:1:1;

[0024] Furthermore, the solubilizing agent is one or more of methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, propylene glycol methyl ether, dipropylene glycol methyl ether, ethylene glycol, propylene glycol, glycerol, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, and vegetable oil derivatives;

[0025] Furthermore, the solubilizing agent is a mixture of isopropanol and ethylene glycol;

[0026] Furthermore, the mass ratio of isopropanol to ethylene glycol is 3:7;

[0027] Further, the active ingredient A, the active ingredient B, the bio-enzyme preparation, the surfactant, the solubilizer and water are stirred and mixed uniformly to prepare an aqueous product with a homogeneous and stable appearance;

[0028] The plant growth regulator of the present invention can be used to control fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0029] Furthermore, the crops are Solanaceae, Cruciferae, Asteraceae, Liliaceae, Vitaceae, Chenopodiaceae, Rutaceae, Bromeliaceae, Rubiaceae, Theaceae, Musaceae, Gramineae and Leguminosae crops;

[0030] The useful plants include, for example, the following types of plants:

[0031] cereals, such as wheat, barley, rye, triticale, durum wheat, oats, hops, rice, corn, millet / sorghum, and maize;

[0032] Beet, such as sugar beet and fodder beet;

[0033] Fruits, such as pome, stone and soft fruits, such as apples, pears, plums, peaches, apricots, cherries and berries (e.g. strawberries, raspberries, blackberries);

[0034] Legumes, such as beans, lentils, peas, and soybeans;

[0035] Oil crops such as rapeseed, mustard, olive, sunflower, coconut, castor, cocoa beans and peanuts;

[0036] melon crops, such as pumpkin, cucumber, bitter melon, watermelon, and cantaloupe;

[0037] fiber plants such as cotton, flax, and jute;

[0038] citrus fruits, such as oranges, lemons, grapefruits, and mandarins;

[0039] Vegetables, such as spinach, lettuce, asparagus, kale, carrots, onions, tomatoes, potatoes, bell peppers, and bell peppers;

[0040] Lauraceae, such as Avocado, Cinnamomum, Camphor;

[0041] Other crops, such as tobacco, nuts, coffee, eggplant, sugar cane, tea, pepper, vines, grapes, hops, bananas, rubber plants, ornamental plants (such as flowers, shrubs, deciduous trees and conifers) and plants used for turf and lawn.

[0042] Furthermore, the crops are oats, rye, triticale, durum wheat, cotton, eggplant, pome fruits, drupes, soft fruits, corn, wheat, barley, cucumber, tobacco, vines, rice, cereals, pears, peppers, beans, soybeans, rapeseed, tomatoes, bell peppers, melons, cabbage, potatoes and apples.

[0043] The present invention also provides a method for using the plant growth regulator as described above, specifically applying an effective dose to crops to be controlled or crop growth areas.

[0044] The plant growth regulator of the present invention has the following advantages:

[0045] The plant growth regulator of the present invention is added with a biological enzyme preparation, and is prepared into various pesticide formulations through conventional formulation processing methods. After cold and heat stability tests and room temperature storage stability tests, a formula with stable physical and chemical properties is screened out. After biological tests to observe the crop conditions, a plant growth regulator with high efficiency, low toxicity, small environmental impact and good safety is obtained. DETAILED DESCRIPTION

[0046] In order to make the present application easier to understand, the present application will be further described in detail below in conjunction with the examples, which are merely illustrative and are not intended to limit the scope of application of the present application. The raw materials or components used in the present application can be obtained through commercial routes or conventional methods unless otherwise specified.

[0047] Preparation Example

[0048] In order to better illustrate the present invention, the content of the present invention is further described below in conjunction with the embodiments. The parts in all the embodiments are parts by weight.

[0049] A plant growth regulator containing biological enzymes, the specific formula composition is as follows:

[0050]

[0051] Preparation method: Active ingredient A, active ingredient B, biological enzyme preparation, surfactant, solubilizer and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0052] A plant growth regulator containing biological enzymes, the specific components are as follows:

[0053]

[0054]

[0055] Preparation method: Sodium nitrophenolate, ammonium ethyl hexanoate, biological enzyme preparation, surfactant, solubilizer and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0056] Preparation Example 1: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0057] formula:

[0058] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0059] 3% biological enzyme preparation (1% superoxide dismutase, 0.5% saccharifying enzyme, 0.5% protease, 0.5% urease, 0.5% cellulase);

[0060] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0061] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0062] Make up the balance with deionized water.

[0063] Preparation method: Sodium nitrophenolate, ammonium ethyl hexanoate, biological enzyme preparation, surfactant, solubilizer and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0064] Preparation Example 2: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0065] formula:

[0066] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0067] 3% biological enzyme preparation (1.5% superoxide dismutase, 0.25% saccharifying enzyme, 0.25% protease, 0.5% urease, 0.5% cellulase);

[0068] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0069] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0070] Make up the balance with deionized water.

[0071] Preparation method: Same as Preparation Example 1.

[0072] Preparation Example 3: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0073] formula:

[0074] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0075] 3% biological enzyme preparation (1.5% superoxide dismutase, 0.5% saccharifying enzyme, 0.5% protease, 0.25% urease, 0.25% cellulase);

[0076] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0077] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0078] Make up the balance with deionized water.

[0079] Preparation method: Same as Preparation Example 1.

[0080] Preparation Example 4: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0081] formula:

[0082] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0083] 3% biological enzyme preparation (1% superoxide dismutase, 0.5% saccharifying enzyme, 0.5% protease, 0.5% urease, 0.5% cellulase);

[0084] 15% surfactant (5% fatty alcohol polyoxyethylene ether, 5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0085] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0086] Make up the balance with deionized water.

[0087] Preparation method: Same as Preparation Example 1.

[0088] Preparation Example 5: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0089] formula:

[0090] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0091] 3% biological enzyme preparation (1% superoxide dismutase, 0.5% saccharifying enzyme, 0.5% protease, 0.5% urease, 0.5% cellulase);

[0092] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0093] 5% solubilizer (1% isopropanol, 4% ethylene glycol);

[0094] Make up the balance with deionized water.

[0095] Preparation method: Same as Preparation Example 1.

[0096] Comparative Example 1: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0097] formula:

[0098] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0099] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0100] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0101] Make up the balance with deionized water.

[0102] Preparation method: Sodium nitrophenolate, ammonium ethyl hexanoate, surfactant, solubilizer and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0103] Comparative Example 2: 3% sodium nitrophenolate·aminoethyl ester aqueous solution

[0104] formula:

[0105] 3% active ingredients (0.6% sodium nitrophenolate, 2.4% ammonium ethyl hexanoate);

[0106] 3% superoxide dismutase;

[0107] 15% surfactant (7.5% fatty alcohol polyoxyethylene ether, 2.5% secondary alcohol polyoxyethylene ether, 5% dodecynyl alcohol polyoxyethylene ether);

[0108] 5% solubilizer (1.5% isopropanol, 3.5% ethylene glycol);

[0109] Make up the balance with deionized water.

[0110] Preparation method: Same as Preparation Example 1.

[0111] Example 1 Impact on tomato quality

[0112] Experiment time and location: The experiment was conducted in the solar greenhouse of Laixi Tomato and Vegetable Experimental Demonstration Station in February 2023.

[0113] Test crops: Tomato (Provence);

[0114] Test materials: Seedlings were raised on September 10, 2022, using 50-hole plug trays; they were planted on October 17, using a randomized block design, with 3 replicates for each treatment and control, and a plot area of ​​124m 2 , the maximum row spacing is 160cm, the minimum row spacing is 40cm, the plant spacing is 27cm, and double-row planting is used.

[0115] Experimental treatments: The experiment set up 7 treatments and 1 clear water control (CK), a total of 8 treatments.

[0116] Table 1 Experimental treatment and dosage

[0117] deal with Experimental treatment Dosage (times of solution) Process 1 3% sodium nitrophenolate·aminoethyl ester aqueous solution (preparation example 1) 1500 Process 2 3% sodium nitrophenolate·aminoethyl ester aqueous solution (preparation example 2) 1500 Process 3 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Preparation Example 3) 1500 Process 4 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Preparation Example 4) 1500 Process 5 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Preparation Example 5) 1500 Process 6 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Comparative Example 1) 1500 Process 7 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Comparative Example 1) 1500 Process 8 Clear water control /

[0118] Experimental treatment

[0119] Seedling stage: spray treatment when there are 2 leaves and 1 heart;

[0120] Treatment during the growth period: spray treatment was performed on 10 plants per replicate, and spraying was performed again after 10 days. Application was performed twice during the growth period, and an equal amount of clean water was used for CK.

[0121] Test determination

[0122] Seedling stage: plant height, stem diameter, and fresh weight of above-ground and underground parts were measured when the seedlings had 4 leaves and 1 heart.

[0123] Three seedlings were randomly selected for each treatment, and the plant height was measured with a ruler, that is, from the stem base to the top growth point of the seedling. The stem diameter was measured 2 cm above the cotyledon with a vernier caliper. The seedlings were cleaned and dried, and the fresh weight of the aboveground and underground parts was measured by an electronic balance.

[0124] Determination of eggplant fruit quality: vitamin C content (determined by colorimetry), soluble sugar content (determined by anthrone colorimetry).

[0125] Data Analysis:

[0126] Excel 2003 software was used for data collation and statistics, and IMB SPSS Statistics 19 software was used for significant difference analysis.

[0127] Test results:

[0128] The effects of treatments 1 to 5 on the growth of tomato seedlings and the fresh weight of the above-ground and underground parts of the seedlings were significantly better than those of treatments 6 and 7, and they promoted the growth and material accumulation of tomato seedlings.

[0129] Table 2 Effects of various experimental treatments on plant height, stem diameter and fresh weight of tomato seedlings

[0130] deal with Plant height / cm Stem diameter / cm Fresh weight (aerial part) / g Fresh weight (underground part) / g Process 1 22.23Aa 5.73Aa 5.50Aa 0.49Aa Process 2 23.29Aa 5.96Aa 5.39Aa 0.46Aa Process 3 24.62Aa 5.69Aa 5.48Aa 0.48Aa Process 4 23.46Aa 5.44Aa 5.61Aa 0.53Aa Process 5 23.19Aa 5.42Aa 5.74Aa 0.54Aa Process 6 18.42Bb 4.65Bb 4.47Bb 0.40Bb Process 7 17.52Bb 4.99Bb 4.53Bb 0.42Bb Process 8 18.38Bb 4.26Cc 3.56Cc 0.36Cc

[0131] Note: Different capital letters indicate significant differences (P<0.01); Different lowercase letters indicate significant differences (P<0.05), the same below.

[0132] Experimental treatments 1 to 5 significantly improved the quality of tomato fruit and could significantly increase the content of vitamin C and soluble sugar.

[0133] Table 3 Effects of various experimental treatments on tomato fruit quality (vitamin C / soluble sugar)

[0134]

[0135]

[0136] Example 2 Tomato Safety Determination

[0137] Experimental location: Tomato greenhouse in Zhucheng, Weifang;

[0138] Trial time: mid-March 2023;

[0139] Test agent: 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Preparation Example 1) 1000 times, 1500 times, 3000 times dilution (water consumption per mu: 45L);

[0140] Test crops: three tomato varieties (Jiafen, Gangliujiu, Liyuan) in the growth period. The growth was consistent, the soil type was consistent, and the water management was appropriate.

[0141] Experimental treatment: Each treatment shall be repeated at least 4 times and each treatment shall have at least 30 tomato plants. Mark the base of the tomatoes with a label (measure accurately with a ruler).

[0142] Experimental treatment: The growth management conditions of each treatment were the same. The pesticide was applied by spraying. The growth of tomato plants in each treatment was investigated and recorded 1, 3, 7 and 10 days after spraying, and the symptoms of pesticide damage were described. The height of new tomato plants was measured on the 21st day.

[0143] Phytotoxicity evaluation criteria:

[0144] Discoloration: including chlorosis, yellowing, albinism, mosaic, rust, browning, greening, etc.

[0145] Necrosis: spots, dead spots, leaf edge necrosis, death of growing points or underground stems, leaf and flower drop, etc.

[0146] Delayed growth and development: dwarfing, inhibited leaf expansion, stagnant growth and development

[0147] Wilting: The plant wilts and withers due to water loss

[0148] Deformities: abnormal tillering, adventitious root growth, lateral growth, excessive elongation, curled or twisted leaves, constricted stems, and deformed flower spikes.

[0149] Color change:

[0150] Crops are considered safe if there are no visible symptoms of pesticide damage after application.

[0151] Less than 10% of leaves have slight discoloration after application, or they will return to safety within 3-4 days;

[0152] After application, 11%-20% of leaves will have obvious discoloration, or they will recover to slight damage within 7-10 days;

[0153] After application, 21%-50% of leaves change color significantly, or cannot recover within 10 days, which is moderate damage;

[0154] After application of the pesticide, more than 50% of the leaves changed color significantly and could not be restored, which was a serious case of pesticide damage.

[0155] Necrosis:

[0156] Crops are considered safe if there are no visible symptoms of pesticide damage after application.

[0157] It is considered safe if less than 10% of the crop organs have a few spots or necrosis, occupying less than 1% of the crop organ area, or if the number of fallen leaves increases by less than 1%;

[0158] If 11%-20% of the crop organs show spots or necrosis, accounting for less than 3% of the crop organ area, or the number of fallen leaves increases by less than 3%, it is considered slight pesticide damage;

[0159] Moderate pesticide damage occurs when 21%-50% of the crop organs show spots or necrosis, occupying less than 10% of the crop organ area, or the leaf fall increases by less than 10%;

[0160] If more than 50% of the crop organs have spots or necrosis, accounting for more than 10% of the crop organ area, or the leaf fall increases by more than 10%, it is considered serious pesticide damage.

[0161] Growth arrest:

[0162] Crops are considered safe if there are no visible symptoms of pesticide damage after application.

[0163] It is safe if the growth rate inhibition rate is within 10% within 21 days after application;

[0164] If the growth rate inhibition rate is 11%-20% within 21 days after application, it is considered as slight injury.

[0165] If the growth rate inhibition rate is within 21%-50% within 21 days after application, it is considered moderate pesticide damage;

[0166] If the growth rate inhibition rate is more than 50% within 21 days after application, it is considered serious pesticide damage.

[0167] Wilting:

[0168] Crops are considered safe if there are no visible symptoms of pesticide damage after application.

[0169] Within 3 days after application, the crop leaves will lose water and wilt for 1-2 hours at noon, and then return to safety;

[0170] Within 3 days after application, crop leaves will lose water for 3h-5h at noon, and recover to slight damage in the morning and evening;

[0171] Within 3-5 hours after application, the leaves of the crops lose water and wilt for more than 5 hours a day, and recover to moderate pesticide damage in the morning and evening;

[0172] If crops wilt after application of pesticides and cannot recover and wilt after 5 days, it is considered serious pesticide damage.

[0173] deformity:

[0174] Crops are considered safe if there are no visible symptoms of pesticide damage after application.

[0175] 21 days after application, if less than 5% of the plants have abnormal tillering, adventitious root growth, lateral growth, leggy growth, curled or twisted leaves, constricted stems, and deformed flower spikes, the pesticide is considered safe.

[0176] If 6%-25% of the plants have abnormal tillering, adventitious root growth, lateral growth, leggy growth, curled or twisted leaves, constricted stems, and deformed flower spikes, it is considered minor phytotoxicity.

[0177] Moderate phytotoxicity is defined as abnormal tillering, adventitious root growth, lateral growth, leggy growth, curled or twisted leaves, constricted stems, and deformed flower spikes in 26% to 50% of the plants.

[0178] More than 50% of the plants have abnormal tillering, adventitious root growth, lateral growth, excessive elongation, curled or twisted leaves, constricted stems, and deformed flower spikes, which are serious pesticide damage.

[0179] The growth inhibition rate of each treatment was calculated based on the survey data with reference to the agricultural industry standard of the People's Republic of China NY / T 1965.1-2010.

[0180] Calculate according to the following formula, and keep the results to two decimal places:

[0181] R=L / D

[0182] R——growth rate / cm·d-1;

[0183] L——the height or length of the plant, branch or new root system / cm;

[0184] D——is time / d.

[0185] R I =(R CK -R T ) / R CK ×100

[0186] R I ——Growth rate inhibition rate / %;

[0187] R CK ——Blank control growth rate / %;

[0188] RT——growth rate after drug treatment / %.

[0189] Test results:

[0190] It was observed that when 3% sodium nitrophenolate·aminoethyl ester aqueous solution was used to spray tomatoes, the plants of three test tomato varieties (Jiafen, Gangliujiu, Liyuan) treated with each concentration were observed and recorded for 1, 3, 7 and 10 days. After the treatment, none of them showed symptoms such as discoloration (including chlorosis, yellowing, albinism, mosaic, rust, browning, greening, etc.), necrosis (including spots, dead spots, leaf edge necrosis, growth point dieback, etc.), delayed growth and development (dwarfing, growth and development stagnation, etc.), wilting (plants wilting due to water loss, etc.), deformity (growth of adventitious roots, lateral growth, leggy growth, leaf curling, etc.), etc., and no acute or chronic damage was found.

[0191] Table 4 Safety test results of each preparation on tomatoes

[0192]

[0193]

[0194] The plant height of tomatoes was measured before and 21 days after application, and the growth inhibition rate of each treatment was calculated. The results in Table 5 show that 1000 times, 1500 times, 3000 times and blank control of 3% sodium nitrophenolate·aminoethyl ester aqueous solution (Preparation Example 1) have a growth promoting effect on tomatoes (Jiafen, Gangliujiu, Liyuan) and are safe for tomatoes.

[0195] Table 5 Results of tomato growth test

[0196]

[0197] Note: The protective effect is the average value of each replicate.

[0198] It should be noted that the embodiments described above are only used to explain the present application and do not constitute any limitation to the present application. The present application is described with reference to typical embodiments, but it should be understood that the words used therein are descriptive and explanatory words, rather than restrictive words. The present application may be modified as specified within the scope of the claims of the present application, and the present invention may be revised without departing from the scope and spirit of the present application. Although the present application described therein relates to specific methods, materials and embodiments, it does not mean that the present application is limited to the specific examples disclosed therein. On the contrary, the present application can be extended to all other methods and applications with the same functions.

Claims

1. A plant growth regulator containing biological enzymes, characterized in that: The plant growth regulator is composed of the following components in weight percentage: active component A 0.05%-5%, active component B 0.1%-10%, biological enzyme preparation 0.5%-10%, surfactant 1%-30%, solubilizer 1%-10%, and deionized water supplemented to 100%.

2. The plant growth regulator according to claim 1, characterized in that The active ingredient A is sodium nitrophenolate and the active ingredient B is ammonium ethyl hexanoate.

3. The plant growth regulator according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 10:1 to 1:

10.

4. The plant growth regulator according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:5 to 5:1; preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:

4.

5. The plant growth regulator according to claim 1, characterized in that: The biological enzyme is at least one or more of superoxide dismutase, saccharifying enzyme, protease, amylase, urease and cellulase.

6. The plant growth regulator according to claim 5, characterized in that The mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 2:1:1:1:1; the mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 6:1:1:2:2; the mass ratio of superoxide dismutase, saccharifying enzyme, protease, urease and cellulase is 6:2:2:1:

1.

7. The plant growth regulator according to claim 1, characterized in that The surfactant of the plant growth regulator is fatty amine polyoxyethylene ether, fatty amine polyoxyethylene ether quaternary ammonium salt, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, isomeric alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, dodecyl alcohol polyoxyethylene ether, alkyl succinamide sulfonate, fatty alcohol polyoxyethylene ether sulfate, C8-C 10 One or more of alkyl polyglycosides; Preferably, the surfactant of the plant growth regulator is fatty alcohol polyoxyethylene ether, secondary alcohol polyoxyethylene ether, and dodecynyl alcohol polyoxyethylene ether.

8. The plant growth regulator according to claim 1, characterized in that The solubilizer is one or more of ethanol, isopropanol, propylene glycol methyl ether, dipropylene glycol methyl ether, ethylene glycol, propylene glycol, and glycerol; preferably, the solubilizer is isopropanol or ethylene glycol.

9. The method for preparing a plant growth regulator according to any one of claims 1 to 8, characterized in that: Active ingredient A, active ingredient B, biological enzyme preparation, surfactant, solubilizer and water are stirred and mixed uniformly to prepare an aqueous product with a homogeneous and stable appearance.

10. The plant growth regulator according to claim 1, characterized in that The plant growth regulator is applied to the crops or crop growth areas to be controlled at an effective dose.

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