Application of orange peel extract in promoting plant growth

Abstract

The invention belongs to the technical field of agriculture, and particularly relates to application of an orange peel extract in plant growth. In the field of agriculture, the use method of the orange peel extract is relatively single, and the invention provides a novel application of the orange peel extract, specifically, the orange peel extract is used as a plant growth promoter for promoting plant growth and improving plant stress resistance.

Description

Technical Field

[0001] The priority information of the present invention is as follows: priority number is 2023114885739, priority date is November 9, 2023, the invention name is application of hesperidin in promoting plant growth, and the disclosure of which is incorporated herein by reference. The present invention belongs to the field of agricultural technology, and specifically relates to the application of orange peel extract in promoting plant growth. Background Art

[0002] Orange peel, called "Chenpi" in traditional Chinese medicine, is a traditional Chinese medicine with a long history. In the Compendium of Materia Medica, there are records about the treatment of various diseases with Chenpi. Modern medical research has also found that orange peel contains a variety of active substances, mainly flavonoid compounds: such as hesperidin, nobiletin, etc., which have antioxidant effects, can remove free radicals in the body, reduce cell oxidative damage, and delay aging; they also have anti-inflammatory effects and can reduce inflammatory reactions. Volatile oil components such as D-limonene have expectorant and cough-relieving effects, which can promote the discharge of respiratory secretions; they also have antibacterial effects, which can inhibit the growth of various bacteria such as Escherichia coli and Staphylococcus aureus, and are used in food preservation and other fields. Phenolic acid compounds such as ferulic acid have antioxidant properties, reduce oxidative stress in the body; and have anti-inflammatory activity, which helps to relieve inflammatory reactions. Carotenoids such as β-carotene are antioxidants that are beneficial to human health. Carbohydrates can activate the plant immune system, enhance the plant's resistance to pathogens, promote the growth and development of plant roots, and eliminate excess free radicals in the plant body, reducing the damage of free radicals to plant cells and thus extending the plant's growth cycle.

[0003] Hesperidin (glycoside) is a flavonoid substance in orange peel extract, with a dihydroflavonoid oxygen glycoside structure, weakly acidic, and pure white needle-shaped crystals. Hesperidin has a variety of biological properties. Modern research has found that hesperidin can resist oxidation and prevent cancer, mildew, anti-allergy, lower blood pressure, inhibit oral cancer and esophageal cancer, maintain osmotic pressure, enhance capillary toughness, and reduce cholesterol. Hesperidin has a broad spectrum of antibacterial effects on common food contaminants, and has a significant inhibitory effect on Bacillus subtilis, Salmonella typhimurium, Shigella flexneri, hemolytic Streptococcus and Vibrio cholerae. Therefore, it is widely used in food additives and food processing. However, the purification of hesperidin during the application process is complicated, costly, and difficult to industrialize.

[0004] In addition, in the agricultural field, there are currently no reports on the use of orange peel extract in regulating plant growth. Summary of the invention

[0005] The study found that orange peel extract not only has a significant promoting effect on plant growth, but also increases the stress resistance of plants. The present invention is the first to discover the effect of orange peel extract in promoting plant growth and improving stress resistance, which expands the application scope of orange peel extract and provides a new option for regulating plant growth in agriculture. The present invention adopts a simple extraction method to obtain orange peel extract, which has a lower application cost. Although the extract contains the hesperidin component mentioned in other patents, the active concentration is much lower than the concentration of hesperidin alone in other patents.

[0006] The present invention provides an application of an orange peel extract containing hesperidin in promoting plant growth, wherein the orange peel is a dry sample of the peel of citrus fruits, such as tangerines, oranges, grapefruits, and the like.

[0007] In the present invention, the promoting of plant growth includes promoting plant germination, promoting the growth of one or more of roots, stems, leaves or flowers and fruits. For example, promoting the growth of root length, stem thickness, plant height, leaf width, leaf length, leaf number, leaf area, biomass, chlorophyll content, yield, etc.

[0008] The present invention also provides an orange peel extract for promoting plant growth. The orange peel extract comprises at least one of an orange peel water extract, a 10%-100% ethanol orange peel extract, and a 100% methanol orange peel extract.

[0009] Furthermore, the orange peel extract is 50%-70% ethanol orange peel extract.

[0010] Furthermore, the orange peel extract is a 60% ethanol orange peel extract, and the components of the orange peel extract include hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, and β-carotene.

[0011] In the present invention, the orange peel extract is used as an active ingredient of the growth promoter and can be used alone or in combination with other products.

[0012] The invention provides a plant growth promoter, which takes orange peel extract as an active ingredient.

[0013] The "growth promoter" mentioned in the present invention refers to a plant growth promoter, which is a biologically active substance that can promote cell division, differentiation and elongation growth, or promote the growth of plant vegetative organs and the development of reproductive organs.

[0014] In the present invention, the orange peel extract is a natural plant growth promoter.

[0015] The plants described in the present invention include but are not limited to economic crops and food crops, such as tobacco, corn, lettuce, wheat, pepper, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear, apple, etc.

[0016] The so-called "economic crops" are of various types, including but not limited to fiber crops (such as cotton, hemp, etc.), oil crops (such as sesame, peanuts, etc.), sugar crops (such as sugarcane, sugar beets, etc.), hobby crops (tobacco), medicinal crops, dye crops, ornamental crops, fruits (such as citrus, kiwi, cherry, pear, apple, etc.) and other economic crops (such as lettuce, pepper, Chinese cabbage, lettuce, Shanghai green), etc.

[0017] The “food crops” mentioned above include, but are not limited to, cereal crops (wheat, rice, corn), tuber crops (including sweet potatoes, potatoes, etc.) and legume crops (including soybeans, broad beans, peas, mung beans, etc.).

[0018] In the product of the present invention, the orange peel extract can be directly used as a single dose. In order to make it stable and convenient for transportation and storage, it can be made into an agricultural product, for example, by adding auxiliary materials to make a corresponding dosage form. The auxiliary materials can be conventional auxiliary materials in the art, for example, dispersants, wetting agents, binders, emulsifiers, stabilizers, solvents, etc.

[0019] On the other hand, the orange peel extract of the present invention can be used as a synergist in combination with foliar fertilizers, water-soluble fertilizers, compound fertilizers, pesticides and other products.

[0020] In the present invention, the dosage form of the agricultural product includes but is not limited to emulsifiable concentrate, suspension concentrate, wettable powder, dust, granule, aqueous solution, mother liquor or mother powder.

[0021] In the present invention, when used, the single agent or agricultural product prepared from the orange peel extract is used to treat seeds, spray leaves or irrigate roots.

[0022] In the present invention, when the orange peel extract or its product is prepared into a solution for promoting seed germination or plant growth, the concentration of the orange peel extract in the solution can be selected according to actual needs.

[0023] In the present invention, the active concentration range of the orange peel extract in the orange peel extract is 0.1-10 ppm, the active concentration range of each main component in the extract is 0.00005-0.2 ppm, among which the active concentration range of main components such as hesperidin, neohesperidin, and nobiletin is 0.001-0.2 ppm.

[0024] The seeds are treated by mixing a single agent or agricultural product made of orange peel extract with seeds to coat the seeds with the orange peel extract, or soaking the seeds to be germinated with the orange peel extract solution.

[0025] In the growth promotion experiment of the present invention, the influence of diseases and insect pests on plant growth has been excluded. Through such an experimental design, the results are sufficient to show that the effect of orange peel extract on promoting plant growth is not caused by avoiding diseases and insect pests.

[0026] The present invention also provides an application of orange peel extract in regulating plant stress resistance.

[0027] Plant stress resistance refers to certain traits that plants possess to resist adverse environments, such as salt-alkali resistance, cold resistance, drought resistance, high temperature resistance, waterlogging resistance, and resistance to dry hot winds.

[0028] In the present invention, the orange peel extract regulates the stress resistance of plants, including at least one of salt and alkali resistance, cold resistance, drought resistance, high temperature resistance, waterlogging resistance, and dry hot wind resistance.

[0029] Beneficial effects of the invention: The invention is the first to discover the effect of orange peel extract in promoting plant growth and improving stress resistance, which expands the application scope of orange peel extract and also provides a new option for regulating plant growth in agriculture. DETAILED DESCRIPTION

[0030] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1 Experiment on promoting wheat root growth by orange peel extract containing hesperidin

[0032] The sample used in this example is an orange peel extract with a hesperidin content of 10%. In the experiment, the orange peel extract is calculated as 100%, and water is used to prepare the concentration of 0.01-200 ppm.

[0033] 1. Experimental design

[0034] Table 1 Experimental design

[0035]

[0036]

[0037] 2. Experimental methods

[0038] After the wheat seeds were soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they were washed with water for 5-6 times, soaked in water for 4-6 hours, and germinated in a constant temperature incubator at 25°C for 1 day. When the wheat seeds turned white, the experimental treatment was carried out. Wheat seeds of uniform size, free of pests and diseases, and with uniform degree of whitening were selected for transplantation into planting baskets. Eight wheat seeds were placed in the planting baskets. Different concentrations of agents were prepared according to the experimental design with Hogland nutrient solution as the base. 200 ml of the prepared agent was added to the bottle so that the wheat seeds just touched the liquid surface. Each treatment was repeated 5 times and placed in a culture room at 25°C for cultivation. The wheat was taken out after 7 days, and the root length and plant height of different treatment groups were measured.

[0039] 3. Experimental results

[0040] As shown in Table 2, the experimental results show that orange peel extract has the effect of promoting wheat growth in the concentration range of 0.01 to 200 ppm. Hydroponics of wheat with different concentrations of agents can significantly promote the growth of wheat roots and aboveground parts, among which the best effect is achieved at a concentration of 0.2 ppm.

[0041] Table 2 Results of orange peel extract promoting wheat root growth

[0042]

[0043] Example 2

[0044] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of deionized water, perform ultrasonic treatment at room temperature for 60 min, repeat the extraction twice, filter, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 1.

[0045] Example 3

[0046] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of ethanol with an ethanol concentration of 10%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 2.

[0047] Example 4

[0048] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of ethanol with an ethanol concentration of 20%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 3.

[0049] Example 5

[0050] Weigh a certain amount of orange peel and grind it into powder, add 20 times the volume of ethanol with an ethanol concentration of 30%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 4.

[0051] Example 6

[0052] Weigh a certain amount of orange peel and grind it into powder, add 20 times the volume of ethanol, the ethanol concentration is 40%, ultrasonicate at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to 1:1 (i.e. 1g of medicinal material corresponds to 1g of extract) to obtain orange peel extract 5.

[0053] Example 7

[0054] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of ethanol with an ethanol concentration of 50%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 6.

[0055] Example 8

[0056] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of ethanol with an ethanol concentration of 60%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 7.

[0057] Example 9

[0058] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of ethanol with an ethanol concentration of 70%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 8.

[0059] Example 10

[0060] Weigh a certain amount of orange peel and grind it into powder, add 20 times the volume of ethanol, the ethanol concentration of which is 80%, ultrasonicate at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to 1:1 (i.e. 1g of medicinal material corresponds to 1g of extract) to obtain orange peel extract 9.

[0061] Embodiment 11

[0062] Weigh a certain amount of orange peel and grind it into powder, add 20 times the volume of ethanol, the ethanol concentration of which is 90%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 10.

[0063] Example 12

[0064] Weigh a certain amount of orange peel and grind it into powder, add 20 times the volume of ethanol, the ethanol concentration of which is 100%, ultrasonicate at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to 1:1 (i.e. 1g of medicinal material corresponds to 1g of extract) to obtain orange peel extract 11.

[0065] Example 13

[0066] Weigh a certain amount of orange peel and grind it into powder. Add 20 times the volume of methanol with a methanol concentration of 100%, perform ultrasonic treatment at room temperature for 30 minutes, repeat the extraction twice, combine the extracts, and concentrate under reduced pressure to a ratio of 1:1 (i.e., 1 g of medicinal material corresponds to 1 g of extract) to obtain orange peel extract 12.

[0067] Test Example 1

[0068] In this test example, potted culture was used to test the effects of the orange peel extracts obtained by different extraction methods in Examples 2-13 on the growth of pakchoi seedlings. In this test example, the orange peel extracts were used by foliar spraying.

[0069] 1. Test methods

[0070] (1) Test samples

[0071] Orange peel water extract (prepared in Example 2), ethanol orange peel extract (prepared in Examples 3 to 12), and methanol orange peel extract (prepared in Example 13), each extract concentration is 100%, and after filtration and sterilization, it is diluted and applied according to the experimental design.

[0072] (2) Experimental design

[0073] There were a total of 37 treatments in the experiment, each repeated 6 times, see Table 3 for details.

[0074] Table 3 Experimental design

[0075]

[0076]

[0077] (3) Test steps

[0078] 1) Preparation of Chinese cabbage test materials

[0079] Weigh equal weight (400 g) of vermiculite and coconut bran sterilized with high pressure steam at 121° C. for 20 minutes, mix them evenly in a ratio of 2:1, and pot them for later use. Soak the Chinese cabbage seeds in 5% sodium hypochlorite solution for 10 minutes, wash them with water 7-8 times, and then sow them; when the Chinese cabbage seedlings grow to 2 leaves and one heart, select seedlings of uniform size and free of pests and diseases for transplantation.

[0080] 2) Preparation of medicine

[0081] The orange peel extracts concentrated by different solvents were prepared into 1% (i.e. 10000 ppm) mother liquor, and the mother liquor was diluted 1000 times, 10000 times, and 100000 times respectively to obtain 10 ppm, 1 ppm, and 0.1 ppm agents.

[0082] 3) Experimental treatment

[0083] When the pakchoi seedlings grew to 3 leaves and one heart, we selected pakchoi seedlings of uniform size, free of pests and diseases, and placed one plant in each pot. Each treatment was repeated 6 times, with a total of 222 pots. According to the experimental design, the leaves of pakchoi in each treatment were evenly sprayed with the pesticide solution, and the application amount was appropriate so that the pesticide would not drip into the soil. The control was sprayed with water. The physiological indicators of pakchoi were investigated 7 days after treatment.

[0084] (4) Survey indicators

[0085] The test indicators include: chlorophyll, leaf area, and fresh weight of aboveground parts.

[0086] 2. Results Analysis

[0087] (1) Effects of different extraction methods of orange peel extract on SPAD value of pakchoy

[0088] As shown in Table 4, compared with the water control, the SPAD values ​​of pakchoi leaves were increased by foliar spraying of different orange peel extracts at different test concentrations. Among them, the SPAD value of pakchoi leaves treated with 60% ethanol extract at 1 ppm was the highest, with an increase rate of 33.04%.

[0089] Table 4 Effects of different orange peel extracts on SPAD values ​​of pakchoy

[0090]

[0091] (2) Effects of different orange peel extracts on the fresh weight of aboveground parts of Chinese cabbage

[0092] As shown in Table 5, the biomass of Chinese cabbage treated with different orange peel extracts increased to varying degrees compared with the water control. Among them, the 60% ethanol extract had the highest aboveground fresh weight at a concentration of 1 ppm, with a growth rate of 37.91%, followed by the 70% ethanol extract at a concentration of 1 ppm, with a growth rate of 34.60%.

[0093] Table 5 Effects of different orange peel extracts on the fresh weight of aboveground parts of Chinese cabbage

[0094]

[0095]

[0096] (3) Effects of different orange peel extracts on the leaf area of ​​Chinese cabbage

[0097] As shown in Table 6, after treatment with different orange peel extracts, the leaf area of ​​​​the pakchoi leaves increased significantly. Among them, the leaf area growth rate under the treatment of 60% ethanol extract 1ppm was the highest, reaching 20.59%, followed by the leaf area growth rate under the treatment of 50% ethanol extract 1ppm, reaching 17.34%.

[0098] Table 6 Effects of different orange peel extracts on the leaf area of ​​Chinese cabbage

[0099]

[0100]

[0101] In summary, orange peel extracts extracted with different solvents have a promoting effect on the growth of pakchoi. The SPAD value, leaf area and biomass of pakchoi leaves are significantly increased. After 60% ethanol extraction, the growth-promoting effect of orange peel extract is more prominent, followed by 50% ethanol extract and 70% ethanol extract, which have better growth-promoting effects. All of them perform better under 1ppm treatment.

[0102] Test Example 2

[0103] In this test example, potted culture was used to test the effects of the orange peel extracts obtained by different extraction methods in Examples 2-13 on rice germination under saline-alkali conditions. In this test example, the orange peel extracts were used as a seed soaking agent.

[0104] 1. Test methods

[0105] (1) Test samples

[0106] The orange peel water extract (prepared in Example 2), the ethanol orange peel extract (prepared in Examples 3 to 12), and the methanol orange peel extract (prepared in Example 13) were filtered and sterilized and then diluted and applied according to the experimental design.

[0107] (2) Experimental design

[0108] There were a total of 37 treatments in the experiment, each repeated 3 times, see Table 7 for details.

[0109] Table 7 Experimental design

[0110]

[0111]

[0112] (3) Experimental treatment

[0113] The rice seeds were surface disinfected with 5% (W / V) sodium hypochlorite solution for 10 minutes, washed with clean water 7-8 times, and the surface moisture was absorbed with filter paper. The seeds were placed in the prepared agent and soaked at room temperature for 24 hours.

[0114] Use saline-alkali soil (pH = 9.12, salt content = 0.11%, strongly alkaline, slightly salinized soil) for sowing. Mix the saline-alkali soil and put it into a germination box, 800g of soil per box, and evenly and slowly add 400g of water. At this time, the saline-alkali soil is basically wet. Use clean tweezers to sow the soaked seeds and press them into the soil about 2cm. There are 45 seeds per pot, and 3 replicates for each treatment. After sowing, cover the soil lightly and place it in a 26℃ culture room for 24 hours of light culture. Rice likes water. During the germination process, observe the soil water conditions at any time and replenish water in time. The amount of water replenished is enough to soak the soil.

[0115] (4) Survey indicators

[0116] After rice began to germinate, the germination rate of each treatment was investigated every day. The final germination rate of each treatment was investigated on the seventh day. The data were processed using Excel and the variance analysis was used to analyze the data differences.

[0117] 2. Results Analysis

[0118] The experimental results are shown in Table 8. From the experimental results, it can be seen that the orange peel extracts obtained by different extraction methods have the effect of promoting the germination of rice seeds under saline-alkali stress in the concentration range of 0.1 to 10 ppm. Using different concentrations of orange peel extracts to soak rice seeds can alleviate the saline-alkali stress of rice. Among them, the rice germination rate under the treatment of 60% ethanol extract 1ppm is the highest, reaching 82.61%, followed by the germination rate under the treatment of 70% ethanol extract 1ppm, reaching 80.59%.

[0119] Table 8 Effects of different orange peel extracts on rice germination under saline-alkali conditions

[0120]

[0121] Test Example 3

[0122] In this test example, the effects of the three orange peel extracts that performed well in Test Examples 1 and 2 on wheat seed germination and seedling growth were tested by potted culture. In this test example, the orange peel extracts were used in hydroponics.

[0123] 1. Test methods

[0124] (1) Test samples

[0125] Ethanol orange peel extract (prepared in Examples 7, 8, and 9) was diluted with water and applied according to the experimental design.

[0126] (2) Experimental design

[0127] There were 10 treatments in total, each repeated 5 times, see Table 9 for details.

[0128] Table 9 Experimental design

[0129]

[0130]

[0131] (3) Experimental treatment

[0132] After the wheat seeds were soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they were washed with water 7-8 times, soaked in water for 4-6 hours, and germinated in a constant temperature incubator at 25℃ for 1 day. When the wheat seeds turned white, the experimental treatment was carried out. Wheat seeds of uniform size, free of pests and diseases, and with uniform degree of whitening were selected for transplantation into planting baskets. Eight wheat seeds were placed in the planting baskets. Different concentrations of agents were prepared according to the experimental design with Hogland nutrient solution as the base. 200 ml of the prepared agent was added to the bottle so that the wheat seeds just touched the liquid surface. Each treatment was repeated 5 times and placed in a culture room with 26℃ for 12 hours of light and 23℃ for 12 hours of darkness. Indicators were investigated after 7 days.

[0133] (4) Survey indicators

[0134] The test investigation indicators include: measuring the root length, plant height, root fresh weight and plant fresh weight of different treatment groups.

[0135] 2. Results Analysis

[0136] The experimental results are shown in Tables 10 and 11. From the experimental results, it can be seen that different orange peel extracts have the effect of promoting the growth of the aboveground part and root system of wheat, and have obvious growth-promoting effects at different concentrations. Among them, the 60% ethanol extraction 1ppm treatment performed the best, with the root length, plant height, root fresh weight, and plant fresh weight growth rates reaching 32.90%, 20.43%, 36.50%, and 22.67%, respectively.

[0137] Table 10 Effect of orange peel extract on root length and plant height of wheat

[0138]

[0139] Table 11 Effects of orange peel extract treatment on root weight and plant weight of wheat

[0140]

[0141]

[0142] Test Example 4

[0143] This test example uses potted culture to test the effects of three ethanol orange peel extracts that performed well in Test Examples 1 and 2 on corn seed germination and seedling growth under saline-alkali conditions. The orange peel extracts in this test example are used as seed soaking agents.

[0144] 1. Test methods

[0145] (1) Test samples

[0146] Ethanol orange peel extract (prepared in Examples 7, 8, and 9) was diluted with water and applied according to the experimental design.

[0147] (2) Experimental design

[0148] There were a total of 10 treatments in the experiment, each repeated 3 times, see Table 12 for details.

[0149] Table 12 Experimental design

[0150]

[0151] (3) Experimental treatment

[0152] 1) Culture matrix preparation

[0153] Weigh an equal weight (200 g) of vermiculite sterilized with high-pressure steam at 121° C. for 20 minutes and put it into pots for later use, for a total of 30 pots. Add 200 ml of saline-alkali aqueous solution (NaCl = 0.3%, Na2SO4 = 2.7%, Na2CO3 = 0.3%, and pH = 9.5 with NaOH) to each pot, stir and mix thoroughly for later use.

[0154] 2) Seed soaking treatment: The corn seeds were surface disinfected with 5% sodium hypochlorite solution for 10 minutes, washed with clean water 7-8 times, and the surface moisture was absorbed with filter paper. 100mL of the 1% mother solution of orange peel extract was prepared according to the use concentration, and 100g of seeds of equal weight were placed in the prepared agent and soaked in a 26℃ incubator for 18 hours. After soaking for 18 hours, the corn seeds were rinsed with clean water and sown in the germination box, 20 seeds were sown per pot, that is, 60 corn seeds were sown for each treatment, and the clean water treatment was used as the control, and placed in a culture room with 26℃ 12h light and 23℃ 12h dark for cultivation. Indicators were investigated after 7 days.

[0155] (4) Survey indicators

[0156] The test investigation indicators include: measuring the germination rate, root length, plant height, root fresh weight and plant fresh weight under different treatments.

[0157] 2. Results Analysis

[0158] (1) Effects of soaking seeds with different orange peel extracts on the germination rate of corn under saline-alkali conditions

[0159] As shown in Table 13, the germination rate of corn increased after soaking with 50%-70% ethanol orange peel extract at 0.1, 1, and 10ppm, among which the germination rate of corn treated with 1ppm was the highest, and the 60% ethanol extraction effect was the best, with the germination rate growth rate reaching 60.82%.

[0160] Table 13 Effect of orange peel extract soaking on corn germination rate under saline-alkali conditions

[0161]

[0162] (2) Effects of soaking seeds with different orange peel extracts on the growth of corn seedlings under saline-alkali conditions

[0163] It can be seen from Tables 14 and 15 that different orange peel extracts have a certain promoting effect on the growth of corn seedlings, and the growth trends of the aboveground part and root system of corn are consistent.

[0164] Overall, the performance of various orange peel extracts was better under the 1ppm treatment, among which the best effect was achieved under 60% ethanol extraction conditions, with the highest growth rates of root length, plant height, root fresh weight, and plant fresh weight reaching 20.90%, 15.45%, 18.81%, and 16.67%, respectively.

[0165] Effects of orange peel extract soaking on maize root growth and plant height under saline-alkali conditions

[0166]

[0167]

[0168] Table 15 Effect of orange peel extract soaking on corn root weight and plant weight under saline-alkali conditions

[0169]

[0170] Test Example 5

[0171] This test example uses potted culture to test the effect of 60% ethanol orange peel extract, which performed well in Test Examples 3 and 4, on the growth of cucumber seedlings by foliar spraying under low temperature conditions. The orange peel extract in this test example is used by foliar spraying.

[0172] 1. Test methods

[0173] (1) Test samples

[0174] 60% ethanol orange peel extract (prepared in Example 8) was diluted with water and applied according to the experimental design.

[0175] (2) Experimental design

[0176] There were a total of 4 treatments in the experiment, each repeated 8 times, and the drug dilution was performed according to the designed concentrations in Table 16.

[0177] Table 16 Experimental design

[0178]

[0179] (3) Experimental treatment

[0180] Prepare vermiculite and coconut bran sterilized with high pressure steam at 121℃ for 20 minutes, mix them in a ratio of 2:1, and put them in pots for later use. Soak cucumber seeds in 5% sodium hypochlorite solution for 10 minutes, wash them with water 7-8 times, and then sow them; when the cucumber seedlings grow to two true leaves that are fully unfolded, select seedlings of uniform size and free of pests and diseases for later use.

[0181] The orange peel extract mother solution was prepared according to the experimental design, and the solution was sprayed until the cucumber leaves were evenly covered with the solution. The application amount was appropriate so that the solution did not drip onto the soil. The clean water treatment was used as the control. 24 hours after spraying, the cucumber seedlings were placed in a 4°C low temperature environment for 24 hours. After the treatment, the cucumber wilting was observed at room temperature and the water loss rate was measured.

[0182] (4) Detection indicators

[0183] The test indicator is the water loss rate of cucumber leaves.

[0184] The first true leaf of the cucumber after low temperature treatment was removed and weighed, and the leaf weight A was recorded. Then, the leaf was placed in clean water for 12 hours to allow the leaf to fully absorb water to saturation, and the leaf weight after water absorption B was recorded.

[0185] Leaf water loss rate % = (BA) / B*100

[0186] 2. Results Analysis

[0187] After low temperature (4°C) treatment, it was observed that the cucumber leaves in the water treatment group were severely wilted, while the cucumber leaves treated with orange peel extract had a lower degree of wilting, without obvious curling and softening. The water loss rate of cucumber leaves under low temperature conditions was used to quantitatively calculate the cold resistance effect. The smaller the water loss rate of the leaves, the better the cold resistance effect.

[0188] As shown in Table 17, the orange peel extract treatment can reduce the water loss rate of cucumber leaves. Among them, the water loss rate of cucumber leaves under the 1 ppm orange peel extract treatment was the smallest, reaching 26.34%, which was 36.27% lower than that of the clean water treatment.

[0189] Table 17 Effect of orange peel extract on water loss rate of cucumber leaf spray under low temperature conditions

[0190]

[0191] Test Example 6

[0192] It can be seen from Test Examples 1-5 that orange peel extract, under 60% ethanol extraction conditions, has a significant effect of promoting growth and alleviating adverse damage on the growth of pakchoi leaves, rice germination under saline-alkali stress, wheat hydroponic growth, corn seed germination under saline-alkali conditions, and cucumber growth under low temperature conditions. Therefore, the 60% ethanol orange peel extract was tested for its components, and the active effects of orange peel extract and each natural component alone on rice seed germination were compared. The method of using the agent in this test example is seed soaking.

[0193] 1. Test methods

[0194] (1) Test samples

[0195] 60% ethanol orange peel extract (prepared in Example 8). The component content of 60% ethanol orange peel extract was measured. The results are shown in Table 18.

[0196] Hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, and β-carotene (pure compounds obtained from natural sources) were diluted with water and applied according to the experimental design.

[0197] Table 18 60% ethanol orange peel extract index component test results

[0198]

[0199] (2) Experimental design

[0200] 60% ethanol orange peel extract, hesperidin (pure compound), neohesperidin (pure compound), nobiletin (pure compound), ferulic acid (pure compound), D-limonene (pure compound) and β-carotene (pure compound) were diluted according to the concentrations designed in Table 19. A total of 8 treatments were conducted in the experiment, and each group was repeated 3 times.

[0201] Table 19 Experimental design

[0202]

[0203]

[0204] (3) Experimental treatment

[0205] The rice seeds were surface disinfected with 1% (W / V) sodium hypochlorite solution for 10 minutes, washed with clean water 5-6 times, and the surface moisture was absorbed with filter paper. The seeds were placed in the prepared agent and soaked in a 26°C incubator for 18 hours.

[0206] According to one treatment, 60mL water + 25g vermiculite is required. Mix the required water and soil, weigh 70g soil and put it into a 10cm×10cm square culture dish, smooth the soil with a plastic sheet, and then plant. Wash the seeds soaked in the agent with clean water to remove the residual agent, use clean tweezers to plant the seeds in the culture dish, press them into the bottom of the dish, 36 seeds per dish, and repeat 3 times for each treatment. After sowing, cover the soil lightly, cover with a lid to keep moisture, and place in a 26℃ culture room under light for culture.

[0207] (4) Detection indicators

[0208] After the rice begins to germinate, the germination rate of each treatment is investigated every day until the germination rate of the drug treatment reaches more than 80%, and the experiment is terminated. The follow-up investigation is usually carried out for 5 days, and the data are processed with Excel and the variance analysis of the data is performed.

[0209] 2. Results Analysis

[0210] The experimental results are shown in Table 20. From the experimental results, it can be seen that the 60% ethanol orange peel extract and the purified hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, and β-carotene all have the effect of promoting rice seed germination in the concentration range of 0.1 to 1ppm, among which the 60% ethanol orange peel extract has the best effect at 1ppm, and the rice germination rate reaches 86.56%. At a concentration of 1ppm, the concentrations of the main components in the 60% ethanol orange peel extract are: hesperidin 0.023ppm, neohesperidin 0.013ppm, nobiletin 0.011ppm, ferulic acid 0.024ppm, D-limonene 0.012ppm, and β-carotene 0.0005ppm, which shows that the effective concentration of each component in the 60% ethanol orange peel extract is significantly lower than the effective concentration of the purified substance alone, which plays a synergistic role.

[0211] Table 20 Effects of orange peel extract and different natural ingredients on rice germination

[0212]

[0213]

[0214] Test Example 7

[0215] The effects of orange peel extract and each natural ingredient alone on rice seed germination under saline-alkali conditions were compared. In this test example, the agent was used for seed soaking.

[0216] 1. Test methods

[0217] (1) Test samples

[0218] 60% ethanol orange peel extract (prepared in Example 8), hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, β-carotene (pure compounds obtained from natural sources) were diluted with water and applied according to the experimental design.

[0219] (2) Experimental design

[0220] There were a total of 8 treatments in the experiment, each group was repeated 3 times, and the drug dilution was performed according to the designed concentrations in Table 21.

[0221] Table 21 Experimental design

[0222]

[0223]

[0224] (3) Experimental treatment

[0225] The rice seeds were surface disinfected with 1% (W / V) sodium hypochlorite solution for 10 minutes, washed with clean water 5-6 times, and the surface moisture was absorbed with filter paper. The seeds were placed in the prepared agent and soaked in a 26°C incubator for 18 hours.

[0226] Prepare an appropriate amount of saline water (NaCl = 0.3%, Na2SO4 = 2.7%, Na2CO3 = 0.3%, pH = 9.5 with NaOH), according to each treatment, 25g of vermiculite + 60mL of saline water. The saline soil used in this test belongs to strong alkaline and moderately salinized soil. After mixing and stirring, weigh 70g of saline soil and put it into a 10cm×10cm square culture dish, and smooth the soil with a plastic sheet.

[0227] After soaking the rice seeds, wash the residual medicine with clean water, and use clean tweezers to put the seeds into the saline-alkali soil and press them into the bottom of the culture dish. There are 36 seeds in each pot of soil, and each treatment is repeated 3 times. After sowing, cover the soil lightly, cover with a lid to keep moisture, and place it in a 26℃ culture room under light.

[0228] (4) Detection indicators

[0229] After rice began to germinate, the germination rate of each treatment was investigated every day. The final germination rate of each treatment was investigated on the seventh day. The data were processed using Excel and the variance analysis was used to analyze the data differences.

[0230] 2. Results Analysis

[0231] The experimental results are shown in Table 22. From the experimental results, it can be seen that 60% ethanol orange peel extract and hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, and β-carotene all have the effect of promoting rice seed germination under saline-alkali stress in the concentration range of 0.1 to 10 ppm. Among them, the rice germination rate was the highest under the 60% ethanol extract treatment of 1 ppm, reaching 83.36%.

[0232] Table 22 Effects of orange peel extract and different natural ingredients on rice germination under saline-alkali conditions

[0233]

[0234]

[0235] Test Example 8

[0236] The effects of orange peel extract and each natural ingredient alone on the growth of lettuce roots under high temperature stress were compared. The agent was used in solid culture medium in this test example.

[0237] 1. Test methods

[0238] (1) Test samples

[0239] 60% ethanol orange peel extract (prepared in Example 8), hesperidin, neohesperidin, nobiletin, and ferulic acid (pure compounds obtained from natural sources) were diluted with water and applied according to the experimental design.

[0240] (2) Experimental design

[0241] There were a total of 8 treatments in the experiment, each group was repeated 3 times, and the agent dilution was performed according to the designed concentrations in Table 23.

[0242] Table 23 Experimental design

[0243]

[0244] (3) Experimental treatment

[0245] A 2% ordinary agar medium (solvent is water) was prepared with the 60% ethanol orange peel extract and other agents shown in Table 23 according to the concentration used, and filled in a square culture dish. Lettuce seeds were sown in the 2% ordinary agar medium, and the lettuce seedlings on the second day after sowing were transplanted to the agar medium added with the 60% ethanol orange peel extract and the like, and the tip of the root was marked at the time of transplantation. The transplanted culture dish was placed at an angle of 45 degrees relative to the bottom surface in a thermostat, and placed at 42°C for 1.5 hours for high temperature treatment. After high temperature treatment, it was cultured at 23°C for 2 days.

[0246] (4) Survey indicators

[0247] The elongation of root length at the root tip of the seedlings after transplantation was measured. The number of measurements in each area was N=15.

[0248] 2. Results Analysis

[0249] The experimental results are shown in Table 24. Compared with the water control, 0.1ppm of 60% ethanol orange peel extract and purified hesperidin, neohesperidin, nobiletin, ferulic acid, D-limonene, and β-carotene all alleviate the inhibitory effect of high temperature stress on the root tip elongation of lettuce seedlings, among which the 60% ethanol orange peel extract treatment has the best alleviating effect. At a concentration of 0.1ppm, the concentrations of the main components in the 60% ethanol orange peel extract are: hesperidin 0.002ppm, neohesperidin 0.001ppm, nobiletin 0.001ppm, ferulic acid 0.002ppm, D-limonene 0.001ppm, and β-carotene 0.00005ppm, which shows that the concentrations of the components in the 60% ethanol orange peel extract are significantly lower than the effective concentration of the purified product alone, and the multiple components of the extract play a synergistic role in the process of resisting high temperature stress.

[0250] Table 24 Effects of orange peel extract and different natural ingredients on root tip elongation of lettuce seedlings under high temperature stress

[0251]

[0252] Test Example 9

[0253] It can be seen from Test Examples 6-8 that hesperidin, neohesperidin, nobiletin, and ferulic acid all have good effects on promoting growth and alleviating adverse damage under 0.1ppm treatment. Therefore, the effects of 60% ethanol orange peel extract and purified hesperidin, neohesperidin, nobiletin, and ferulic acid alone on the growth of corn seedlings under waterlogging stress were compared under 0.1ppm treatment. The use of the agent in this test example is foliar spraying.

[0254] 1. Test methods

[0255] (1) Test samples

[0256] 60% ethanol orange peel extract (prepared in Example 8), hesperidin, neohesperidin, nobiletin, and ferulic acid (pure compounds obtained from natural sources) were diluted with water and applied according to the experimental design.

[0257] (2) Experimental design

[0258] There were a total of 6 treatments in the experiment, each group was repeated 3 times, and the drug dilution was performed according to the designed concentrations in Table 25.

[0259] Table 25 Experimental design

[0260]

[0261] (3) Experimental treatment

[0262] Select corn seeds of uniform size, disinfect with 5% sodium hypochlorite for 10 minutes, rinse with water, soak in water for 4-6 hours, germinate in a constant temperature incubator at 25℃ for 1 day, select corn seeds with uniform germination potential and transplant into planting baskets, put 10 corn seeds in the planting baskets, add 500ml of tap water to the bottle, so that the corn seeds just touch the liquid surface, repeat 3 times for each treatment, and place in a culture room with 26℃ 12h light and 23℃ 12h dark for cultivation. After culturing with tap water for 3 days, add 1 / 2 Hoagland nutrient solution 1:1000 times liquid, and after the corn is cultivated to the three-leaf and one-heart stage, spray the prepared agents for each treatment according to the experimental design until the corn leaves are evenly covered with the liquid, but the liquid does not drip down, and spray clean water for the control. Then raise the culture water level to 5 cm from the base of the corn seedling to the stem, and investigate the indicators after 7 days.

[0263] (4) Survey indicators

[0264] The test investigation indicators include: measuring the plant height and root length of different treatment groups.

[0265] 2. Results Analysis

[0266] The experimental results are shown in Table 26. It can be seen from the experimental results that 0.1ppm of 60% ethanol orange peel extract and purified hesperidin, neohesperidin, nobiletin, and ferulic acid all have the effect of promoting the growth of corn seedlings under waterlogging stress. Among them, the 60% ethanol orange peel extract treatment has the best effect, and the growth rates of corn root length and plant height reached 14.64% and 12.56% respectively.

[0267] Table 26 Effects of orange peel extract and different natural ingredients on the growth of corn seedlings under waterlogging stress

[0268]

[0269] Test Example 10

[0270] It can be seen from Test Examples 1 to 9 that the 60% ethanol orange peel extract has a significant effect of promoting growth and alleviating damage caused by adverse conditions. Therefore, the 60% ethanol orange peel extract was used with surfactants such as thickeners and emulsifiers to prepare an orange peel extract preparation to verify the effect of the stable preparation on crop growth.

[0271] 1. Trial preparation of 60% ethanol orange peel extract

[0272] The formulation includes: orange peel extract, thickener-xanthan gum, emulsifier-alkyl glycoside, defoamer and water, which are compounded and trial-produced according to different proportions. The specific formula design is shown in Table 27:

[0273] Table 27 Trial formulation of orange peel extract preparation

[0274]

[0275] 2. Stability test of trial formula

[0276] After trial production of each formulation, the samples were subjected to high temperature (54°C) and low temperature (0°C) storage stability tests, and the formulation stability investigation was conducted after the samples were stored in hot and cold conditions for 2 weeks.

[0277] The results showed that Formulation 1 precipitated under both hot and cold storage conditions; Formulation 2 samples showed obvious stratification without the addition of emulsifiers and had poor water dispersibility; Formulation 3 samples had more bubbles during the stirring and dissolving process; Formulation 4 preparation samples were homogeneous stable liquids with good water dispersibility.

[0278] Therefore, formulation 4 was selected as the trial formulation for orange peel extract.

[0279] 3. Formulation trial production process

[0280] Take 60% ethanol orange peel extract, thickener, emulsifier, defoamer and water in the ratio of 1:5:3:0.5:90.5 for formula trial production. The specific process operation is as follows:

[0281] ① Weigh 3% xanthan gum and add it to 90.5% water for shearing until the xanthan gum is completely dissolved and dispersed into a

[0282] Make it into a uniform liquid for later use;

[0283] ② The liquid in ① is stirred at 55° C., and 1% of orange peel extract is accurately weighed and added into a stirring kettle, and stirred at 55° C. for 10 min until the liquid is homogeneous;

[0284] ③ Weigh 5% of alkyl polyglycoside and add it to the above ② homogeneous liquid, stir for 5 minutes; then add 0.5% of defoaming agent and stir for 3 minutes to complete the trial production of the formula sample.

[0285] 4. Orange peel extract preparation testing

[0286] The basic physicochemical properties and technical indicators of orange peel extract preparations are shown in Table 28:

[0287] Table 28 Orange peel extract preparation test results

[0288]

[0289] After forming a stable sample of the orange peel extract preparation, the preparation was applied to indoor and outdoor biological test effect verification to clarify its application effect.

[0290] Test Example 11

[0291] This test example tests the effect of the orange peel extract preparation in Test Example 10 on the growth and quality of pakchoi seedlings by pot culture. The orange peel extract preparation in this test example is used by foliar spraying.

[0292] 1. Test methods

[0293] (1) Test samples

[0294] The orange peel extract preparation prepared in Test Example 10, in which the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0295] (2) Experimental design

[0296] There were a total of 4 treatments in the experiment, each repeated 6 times, see Table 29 for details.

[0297] Table 29 Experimental design

[0298]

[0299] (3) Experimental treatment

[0300] Select the pakchoi seedlings (3 true leaves) with consistent growth, no diseases and insect pests, and 6 seedlings in each treatment group. According to the experimental design, the orange peel extract preparation was prepared with water to 0.1, 1, and 10ppm, and the pakchoi was sprayed on the leaves respectively, so that the liquid was evenly distributed on the leaf surface but not dripping, and placed in the culture room for cultivation. The temperature was set to 2 periods: 26℃14h light, 23℃10h dark. The number of medications was 2 times, with an interval of 7 days. During the experiment, the water and fertilizer management of each treatment group was consistent. After 14 days, the SPAD value, fresh weight, number of leaves, and leaf area of ​​the leaves in different treatment groups were investigated.

[0301] 2. Results Analysis

[0302] The experimental results are shown in Table 30. From the experimental results, it can be seen that orange peel extract has the effect of promoting the growth of Chinese cabbage in the concentration range of 0.1 to 10 ppm. Spraying the leaves of Chinese cabbage with orange peel extract of different concentrations can promote the accumulation of Chinese cabbage biomass and increase the chlorophyll content of the leaves. The best effect is achieved at a concentration of 1 ppm.

[0303] Table 30 Results of orange peel extract preparation promoting the growth of Chinese cabbage

[0304]

[0305]

[0306] Test Example 12

[0307] In this test example, the effect of the orange peel extract preparation prepared in Test Example 10 on the growth of pepper seedlings was tested by pot culture. In this test example, the orange peel extract preparation was used by foliar spraying.

[0308] 1. Test methods

[0309] (1) Test samples

[0310] The orange peel extract preparation (prepared in Test Example 10), wherein the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0311] (2) Experimental design

[0312] There were a total of 4 treatments in the experiment, each repeated 6 times, see Table 31 for details.

[0313] Table 31 Experimental design

[0314]

[0315] (3) Experimental treatment

[0316] After the pepper seeds are soaked and disinfected in 5% sodium hypochlorite solution for 10 minutes, they are washed with water 5-6 times and then sown; when the pepper seedlings grow to 4-5 leaves, seedlings of uniform size and free of pests and diseases are selected for planting and standby use.

[0317] According to the experimental design, orange peel extract preparations were prepared with water to 0.1, 1, and 10 ppm. Pepper seedlings of uniform size and free of pests and diseases were selected, with one plant per pot, and each treatment was repeated 6 times. After 3 days of seedling acclimatization, the prepared agents of each treatment were sprayed until the pepper leaves were evenly covered with the liquid, but the liquid did not drip down. The control was sprayed with water. The plants were placed in a culture room for cultivation, and the temperature was set to 2 periods: 26℃14h light, 23℃10h dark. During the experiment, the water and fertilizer management of each treatment group was consistent, and the indicators were investigated 7 days after the treatment.

[0318] The test indicators include: leaf number, root length, plant height, root weight and plant weight

[0319] 2. Results Analysis

[0320] As shown in Table 32, the foliar spraying of orange peel extract in the concentration range of 0.1 to 10 ppm has a significant promoting effect on the growth of pepper seedlings. The aboveground biomass and root system of peppers have increased significantly compared with the water treatment, and the 1 ppm concentration treatment has the best effect on the growth of pepper seedlings.

[0321] Table 32 Effect of orange peel extract treatment on the growth of pepper seedlings

[0322]

[0323] Test Example 13

[0324] This test example uses potted culture to test the effect of the orange peel extract preparation in Test Example 10 on the growth and quality of wheat seedlings under high temperature stress. The orange peel extract preparation in this test example is used by foliar spraying.

[0325] 1. Test methods

[0326] (1) Test samples

[0327] The orange peel extract preparation prepared in Test Example 10, in which the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0328] (2) Experimental design

[0329] There were a total of 4 treatment groups in the experiment, each group was repeated 3 times, see Table 33 for details.

[0330] Table 33 Experimental design

[0331]

[0332] (3) Experimental treatment

[0333] Prepare vermiculite and coconut bran sterilized at 121℃ high pressure steam for 20 minutes, mix them evenly in a ratio of 2:1, and pot them for later use. Soak the wheat seeds in 5% sodium hypochlorite solution for 10 minutes, then wash them with water 7-8 times. Soak them in water for 4-6 hours, germinate them in a constant temperature incubator at 25℃ for 1 day, and plant them when the wheat seeds are white. Select wheat seeds of the same size, free of pests and diseases, full and with the same degree of whitening, and plant them in pots. Plant 16 seeds per pot, repeat 5 times for each treatment, and place them in a culture room with 26℃ 12h light and 23℃ 12h darkness for cultivation. Water them according to the growth situation. On the third day after the wheat seedlings emerge, spray the prepared agents for each treatment according to the experimental design until the wheat leaves are evenly covered with the liquid, but the liquid does not drip down, and spray water for the control. It was placed in a culture room for cultivation, and the temperature was set to two periods: 34°C for 14 hours of light, and 28°C for 10 hours of darkness. The water and fertilizer management of each treatment group was consistent during the experiment, and the indicators were investigated after 5 days of treatment.

[0334] (4) Survey indicators

[0335] The test investigation indicators include: measuring the plant height, root length, plant fresh weight and root fresh weight of different treatment groups.

[0336] 2. Results Analysis

[0337] The experimental results are shown in Table 34. From the experimental results, it can be seen that the orange peel extract preparation has the effect of promoting the growth of wheat under high temperature stress in the concentration range of 0.1 to 10 ppm. Spraying the orange peel extract preparation at different concentrations on the wheat leaves can alleviate the high temperature damage of wheat and promote the accumulation of wheat biomass, among which the best effect is achieved at a concentration of 1 ppm.

[0338] Table 34 Effect of orange peel extract preparation on wheat seedling growth under high temperature stress

[0339]

[0340] Test Example 14

[0341] This test example tests the effect of the orange peel extract preparation in Test Example 10 on the growth of corn seedlings under drought stress by pot culture. The orange peel extract preparation in this test example is used as a seed soaking agent.

[0342] 1. Test methods

[0343] (1) Test samples

[0344] The orange peel extract preparation prepared in Test Example 10, in which the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0345] (2) Experimental design

[0346] There were a total of 4 treatment groups in the experiment, each group was repeated 6 times, see Table 35 for details.

[0347] Table 35 Experimental design

[0348]

[0349] (3) Experimental treatment

[0350] Select corn seeds of uniform size and disinfect them with 5% sodium hypochlorite for 10 minutes, then rinse them with water and dry the surface moisture with filter paper. Orange peel extract preparations are prepared into 100mL of the agent according to the designed concentration, and 100g of seeds of equal weight are placed in the prepared agent respectively. The water treatment is the control, and the seeds are soaked in a 26℃ incubator for 18 hours. After soaking the corn seeds for 18 hours, rinse them with water, select seeds with consistent seed size and germination potential and sow them in the germination box, sow 15 seeds per pot, and repeat each treatment 3 times. After culturing with tap water for 3 days, add 1 / 2 Hoagland nutrient solution 1:1000 times solution, place it in the culture room for cultivation, set the temperature to 25℃, and light / darkness to 12h / 12h. Change the water every 3 days. After the corn is cultured to the three-leaf and one-heart stage, the nutrient solution is replaced with 20% PEG-6000 stress. After stressing for 7 days, the agronomic traits of the seedlings are measured.

[0351] (4) Survey indicators

[0352] The test investigation indicators include: measuring the SPAD value, plant height, root length, plant fresh weight and root fresh weight of different treatment groups.

[0353] 2. Results Analysis

[0354] The experimental results are shown in Table 36. From the experimental results, it can be seen that the orange peel extract preparation has the effect of promoting corn growth in the concentration range of 0.1 to 10 ppm. Using the orange peel extract preparation to soak corn seeds can alleviate the damage of drought stress to corn seedlings, improve the ability of corn to resist drought stress, promote the accumulation of corn biomass, and increase the chlorophyll content of leaves. The best effect is achieved at a concentration of 1 ppm.

[0355] Table 36 Effect of orange peel extract preparation on corn seedling growth under drought stress

[0356]

[0357]

[0358] Test Example 15

[0359] The test site of this test example is a citrus planting area in Daxing Town, Pujiang County, Chengdu City, Sichuan Province. The effect of the orange peel extract preparation prepared in Test Example 10 combined with potassium dihydrogen phosphate foliar spray on citrus fruit yield and quality was tested.

[0360] 1. Test methods

[0361] (1) Test samples

[0362] The orange peel extract preparation (prepared in Test Example 10), wherein the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0363] (2) Experimental design

[0364] There were a total of 4 treatments in the experiment, each repeated 3 times, see Table 37 for details.

[0365] Table 37 Experimental design

[0366]

[0367] (3) Experimental treatment

[0368] Twelve citrus trees with uniform tree vigor were selected and divided into four plots, with three trees per plot as one treatment plot, and arranged in random blocks. On the basis of conventional fertilization, the experiment adopted the method of foliar spraying of water-soluble fertilizers, and set up four treatments of spraying clean water (CK), potassium dihydrogen phosphate, orange peel extract preparation, and orange peel extract preparation + potassium dihydrogen phosphate, with three repetitions. The agent was prepared with water to the use concentration according to the experimental design, and water-soluble fertilizer was sprayed once during the budding period, flowering period, and fruit expansion period of citrus, respectively, on the leaves and back of the leaves, with water droplets appearing on the leaves but not dripping. Except for spraying foliar fertilizers, other cultivation and management measures in the experimental plots were consistent.

[0369] (4) Measurement indicators and methods

[0370] After the fruits matured, 8 fruits were randomly selected from the outer middle part of the tree at 4 different directions. 24 fruits were treated in each treatment and the average single fruit weight, longitudinal diameter, transverse diameter, Vc content, total sugar, titratable acid content, soluble solids content, number of fruits per plant and yield were measured.

[0371] 2. Results Analysis

[0372] (1) Effects of different treatments on citrus fruit traits

[0373] As shown in Table 38, the orange peel extract preparation combined with potassium dihydrogen phosphate has a significant effect on the single fruit weight, longitudinal diameter and transverse diameter of the fruit. Compared with the use of potassium dihydrogen phosphate alone, the addition of orange peel extract preparation can significantly increase the single fruit weight of citrus. The single fruit weight, longitudinal diameter, transverse diameter and fruit shape index of the orange peel extract preparation combined with potassium dihydrogen phosphate are significantly better than those treated with water.

[0374] Table 38 Effects of different treatments on citrus fruit traits

[0375]

[0376] Note: Different lowercase letters in the same column indicate significant differences (P<0.05). The same below.

[0377] (2) Effects of different treatments on citrus yield

[0378] As shown in Table 39, the orange peel extract preparation combined with potassium dihydrogen phosphate significantly increased the yield of citrus. The number of fruits per plant in the treatment of spraying orange peel extract preparation + potassium dihydrogen phosphate was the largest, at 226, significantly higher than other treatments. The number of fruits per plant in the treatment of orange peel extract preparation alone was significantly higher than that of potassium dihydrogen phosphate alone and water treatment. The yield of the treatment of spraying orange peel extract preparation + potassium dihydrogen phosphate was the highest, at 15270.31 kg·hm -2 , significantly higher than other treatments.

[0379] Table 39 Effects of different treatments on citrus yield

[0380]

[0381] (3) Effects of different treatments on citrus fruit quality

[0382] As shown in Table 40, the orange peel extract preparation combined with potassium dihydrogen phosphate has a significant effect on the quality of citrus fruit. The VC content of the fruit treated with the orange peel extract preparation + potassium dihydrogen phosphate is the highest, significantly higher than that of other treatments; the soluble solids are significantly higher than those of other treatments. Compared with potassium dihydrogen phosphate alone, spraying the orange peel extract preparation can significantly reduce the content of titratable acid in the fruit and increase the total sugar content.

[0383] Table 40 Effects of different treatments on citrus fruit quality

[0384]

[0385] Test Example 16

[0386] The test site of this test example is a wheat planting field in Zhenping County, Nanyang City, Henan Province. The effect of the orange peel extract preparation prepared in Test Example 10 on the growth and quality of wheat under dry hot wind conditions was tested. The orange peel extract preparation in this test example was used by foliar spraying.

[0387] 1. Test methods

[0388] (1) Test samples

[0389] The orange peel extract preparation (prepared in Test Example 10), wherein the orange peel extract was added in an amount of 1% (i.e., the concentration of the effective substance was 10,000 ppm), was diluted with water and applied according to the test design.

[0390] (2) Experimental design

[0391] There were a total of 4 treatments in the experiment, each repeated 3 times, see Table 41 for details.

[0392] Table 41 Experimental design

[0393]

[0394] (3) Experimental treatment

[0395] Wheat fields with uniform growth were selected. On the basis of conventional fertilization, water-soluble fertilizer was sprayed on the leaves. Four treatments were set up, including spraying clean water (CK) and orange peel extract preparations of different concentrations. Each treatment was repeated three times. The plot area was 3m×3m=9m 2, arranged in random blocks. The agent was prepared with water to the use concentration according to the experimental design, and water-soluble fertilizer was sprayed once from the wheat greening stage to the jointing stage and from the booting stage to the early filling stage. The middle and upper stems and leaves of wheat were sprayed evenly and carefully with a sprayer, and water droplets appeared on the leaf surface but did not drip. Except for spraying foliar fertilizer, other cultivation and management measures in the experimental plot were the same.

[0396] (4) Measurement indicators and methods

[0397] The SPAD value, SOD, POD activity and MDA content of wheat flag leaves were measured 7 days after the second spraying.

[0398] Yield: Each replicate was harvested manually, air-dried, threshed, and weighed. The standard yield was calculated based on a moisture content of 13%.

[0399] 2. Results Analysis

[0400] (1) Effects of different treatments on SPAD values ​​of wheat leaves under dry hot wind conditions

[0401] As shown in Table 42, compared with the water treatment, the orange peel extract preparation increased the flag leaf SPAD value in the concentration range of 0.1-10ppm, and the SPAD value growth rate reached 21.91% at a concentration of 1ppm. Spraying wheat with the orange peel extract preparation can alleviate the damage to the flag leaf of wheat caused by dry hot wind stress, increase the chlorophyll content of the flag leaf, and promote wheat growth.

[0402] Table 42 Effects of different treatments on SPAD values ​​of wheat leaves under dry hot wind conditions

[0403]

[0404] (2) Effects of different treatments on physiological parameters of wheat leaves under dry hot wind conditions

[0405] As shown in Table 43, compared with the clean water treatment, the orange peel extract preparation can enhance the SOD and POD activities of leaves and reduce the MDA content in the concentration range of 0.1 to 10 ppm, with the best effect at a concentration of 1 ppm.

[0406] Table 43 Effects of different treatments on physiological parameters of wheat leaves under dry hot wind conditions

[0407]

[0408]

[0409] (3) Effects of different treatments on wheat yield under dry hot air conditions

[0410] It can be seen from Table 44 that, compared with the clean water treatment, the orange peel extract preparation can increase wheat yield within the concentration range of 0.1 to 10 ppm, among which the best effect is achieved at a concentration of 1 ppm, with a yield growth rate of 9.29%.

[0411] Table 44 Effects of different treatments on wheat yield under dry hot wind conditions

[0412]

[0413] Although the embodiments and test examples of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. The application of orange peel extract containing hesperidin in promoting plant growth.

2. An orange peel extract for promoting plant growth, characterized in that: The orange peel extract comprises at least one of orange peel water extract, 10%-100% ethanol orange peel extract, and 100% methanol orange peel extract.

3. The orange peel extract for promoting plant growth according to claim 2, characterized in that The orange peel extract is 50%-70% ethanol orange peel extract.

4. The orange peel extract for promoting plant growth according to claim 3, characterized in that The active concentration of the orange peel extract in the orange peel extract ranges from 0.01 to 200 ppm.

5. The orange peel extract for promoting plant growth according to claim 4, characterized in that The active concentration of the orange peel extract in the orange peel extract is in the range of 0.1-10 ppm.

6. The orange peel extract for promoting plant growth according to claim 2 or 3, characterized in that: The orange peel extract is a 60% ethanol orange peel extract, and the components of the orange peel extract include flavonoid compounds, phenolic acid compounds, volatile oils, sugars, vitamins, carotenoids, and trace elements.

7. The orange peel extract for promoting plant growth according to claim 6, characterized in that The flavonoids include hesperidin, neohesperidin and nobiletin; the phenolic acid compounds include ferulic acid; the volatile oils include D-limonene; and the carotenoids include beta-carotene.

8. The orange peel extract for promoting plant growth according to claim 6, characterized in that: The active concentration range of the orange peel extract component is 0.00005-0.2 ppm.

9. The orange peel extract for promoting plant growth according to claim 7, characterized in that The active concentration range of the hesperidin, neohesperidin and nobiletin is 0.001-0.2 ppm.

10. The use according to claim 1, characterized in that: Orange peel is the dried peel of citrus fruits.

11. The use according to claim 1, characterized in that: The promoting plant growth includes promoting seed germination, and / or promoting the growth of one or more of roots, stems, leaves, or flowers and fruits.

12. The use according to claim 1, characterized in that: When in use, the orange peel extract is made into an agricultural product for use, and the agricultural product also includes auxiliary materials, which are one or more of a dispersant, a wetting agent, a binder, an emulsifier, a stabilizer, and a solvent.

13. The use according to claim 12, characterized in that: The dosage form of the product is emulsifiable concentrate, suspension, wettable powder, dust, granule, aqueous solution, mother liquid or mother powder.

14. The use according to claim 13, characterized in that: The agricultural product made from orange peel extract is used in combination with foliar fertilizer, water-soluble fertilizer, compound fertilizer and pesticide.

15. The use according to claim 14, characterized in that: When using, treat seeds, spray leaves or irrigate roots with a single dose of orange peel extract or the prepared agricultural product.

16. A plant growth promoter, characterized in that It has orange peel extract as its active ingredient.

17. Application of orange peel extract in regulating plant stress resistance.

18. The use according to claim 17, characterized in that The stress resistance is at least one of salt-alkali resistance, cold resistance, drought resistance, high temperature resistance, waterlogging resistance, and dry hot wind resistance.

19. The use or growth promoter according to any one of claims 1 to 18, characterized in that: The plants include tobacco, corn, lettuce, wheat, pepper, Chinese cabbage, lettuce, bok choy, tomato, citrus, kiwi, cherry, pear and apple.

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