Application of feed for improving reproductive performance of sows

Abstract

The invention belongs to the technical field of animal reproduction, and discloses application of a feed for improving reproductive performance of sows. According to the invention, the eucommia ulmoides extract is applied to the daily ration of the sows at the early stage of pregnancy for the first time, and it is found that the eucommia ulmoides extract can significantly improve the activity of T-AOC, SOD and CAT in serum of the sows, the blastocyst EdU positive cell rate, the diversity of intestinal flora alpha and the relative abundance of beneficial bacteria; the serum MDA content of the sows and the relative abundance of harmful bacteria in intestinal tracts are reduced, and the total litter size, the total live litter size, the live birth weight, the live birth litter weight and the stillbirth rate of the sows are remarkably influenced. And the economic benefit of the farm is improved to a great extent.

Description

Technical Field

[0001] The invention relates to application of feed for improving sow reproductive performance, belonging to the technical field of animal reproduction. Background Art

[0002] Pregnant sows are prone to produce a large amount of reactive oxygen species (ROS) in their bodies due to their vigorous metabolism. Although ROS are normal products of cell metabolism, if excessive ROS cannot be removed quickly and effectively, it will cause a large amount of oxidative stress in the body, hindering the subsequent development potential of the embryo and reducing the survival rate of piglets. Early pregnancy is the main period of embryonic cell division, growth, differentiation and implantation, which determines the quality of embryo development and the production performance of sows. Therefore, reducing oxidative stress in the body of sows in early pregnancy is one of the effective ways to promote embryonic development and improve sow production performance. In addition, the reproductive performance of sows is affected by genetic background, environmental conditions, nutritional status and other factors. Intestinal flora is an important factor in maintaining the stability of the host's intestinal environment, and one of the main ways it interacts with the host is through metabolites. These metabolites are produced as intermediates or end products of microbial metabolism. These signals from microorganisms can affect various stages of female reproduction by affecting host nutritional metabolism, energy balance, immune homeostasis, etc., including follicle and oocyte maturation, fertilization, implantation and embryo migration, thereby leading to differences in reproductive performance.

[0003] Eucommia ulmoides is a traditional Chinese medicinal material with abundant resources. Among them, Eucommia ulmoides leaf extract has been included in the catalog of feed additives and has good safety. Recent studies have shown that Eucommia ulmoides bark, Eucommia ulmoides leaf extract and Eucommia ulmoides leaf powder can improve the health level and production performance of livestock, poultry and fish; Eucommia ulmoides extract has broad-spectrum antibacterial, antioxidant, free radical scavenging and immunity-enhancing effects, and has a certain effect on improving the health of high-age chickens and delaying the decline in egg production, and has the potential to improve the quality of animal meat and eggs. In addition, Eucommia ulmoides extract contains a high content of chlorogenic acid. Chlorogenic acid, chemical formula C 16 H 18 O9, which has multiple biological activities such as antioxidant, antibacterial and anti-inflammatory, is a new type of highly effective natural phenolic antioxidant. Therefore, Eucommia extract has the potential to develop new antioxidants for feed. However, it is still unclear whether Eucommia extract can improve the reproductive performance of sows by participating in regulating the composition of intestinal flora in early pregnancy sows and improving the quality of embryonic development. Therefore, supplementing Eucommia extract in the diet of sows in early pregnancy may be of great significance for regulating the composition of intestinal flora in sows, improving sow production performance and improving the economic benefits of farms. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide an application of feed for improving the reproductive performance of sows, which is simple to operate, green, environmentally friendly and safe, and has a good effect on improving the production performance of sows.

[0005] The invention provides an application of a feed for improving the reproductive performance of sows. The feed is a basic diet to which an eucommia extract containing 50% chlorogenic acid is added.

[0006] Preferably, the basal diet is a commercial diet.

[0007] Preferably, the feed feeding stage is the early pregnancy of the sow, and the feeding amount of the Eucommia ulmoides extract is 600-2000 mg / kg / day.

[0008] Preferably, the early pregnancy of the sow is selected from the 1st to 15th day or the 16th to 30th day of pregnancy.

[0009] Preferably, the early pregnancy of the sow is selected from the 1st to 15th day of pregnancy.

[0010] Preferably, the feeding amount of the Eucommia ulmoides extract is 2000 mg / kg / day.

[0011] The invention discloses an application of feed for improving reproductive performance of sows, which also includes improving production performance of sows and enhancing antioxidant capacity of sows in early pregnancy. Improving production performance of sows includes increasing total number of piglets born, total number of piglets born alive, live piglet birth weight, live piglet birth litter weight, and reducing stillbirth rate. Improving antioxidant capacity of sows in early pregnancy includes increasing T-AOC, SOD and CAT activities in serum of sows, and reducing MDA content in serum of sows.

[0012] The present invention discloses an application of feed for improving reproductive performance of sows, which also includes regulating the alpha diversity and composition of intestinal flora of pregnant sows in the early stage. The regulating alpha diversity of intestinal flora of pregnant sows in the early stage includes improving Chao1, Shannon, Observed-species, Faith-pd and Pielou-e index of fecal flora of sows; the regulating composition of intestinal flora of pregnant sows in the early stage includes reducing the relative abundance of Terrisporobacter, Turicibacter, Clostridium_T, Romboutsia_B and Streptococcus in fecal flora of sows, and increasing the relative abundance of UBA4334, Treponema_D, Prevotella, Cryptobacteroides and Sodaliphilus in fecal flora of sows.

[0013] The invention discloses an application of feed for improving sow reproductive performance, and also includes improving the quality of early embryonic development of sows, wherein improving the quality of early embryonic development of sows includes increasing the EdU positive cell rate of blastocysts.

[0014] The present invention also discloses a feeding method for improving the reproductive performance of sows, comprising feeding the sows with eucommia extract or feed containing 50% chlorogenic acid eucommia extract on the 1st to 15th day of pregnancy, wherein the feeding amount of the eucommia extract is 2000 mg / kg / day.

[0015] Beneficial effects:

[0016] 1. Eucommia extract has a significant effect on improving the production performance of sows.

[0017] 2. Eucommia extract can significantly improve the antioxidant capacity of sows in early pregnancy and improve the quality of early embryonic development in sows.

[0018] 3. Eucommia extract can significantly increase the richness and uniformity of fecal flora in pregnant sows, reduce the level of intestinal inflammation in sows and the relative abundance of bacteria related to induced oxidative stress, increase the relative abundance of bacteria related to energy metabolism, and improve the intestinal health and energy metabolism of sows.

[0019] 4. Eucommia extract is green and environmentally friendly, often used as a food additive, and has no side effects on sows. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of sow production performance of the present invention; wherein Figure 1 A is the total number of piglets born by sows; Figure 1 B is the total number of piglets born alive; Figure 1 C is the stillbirth rate; Figure 1 D is the live birth weight; Figure 1 E is the live litter weight at birth; Figure 1 F is the coefficient of variation of birth weight within a litter of live pups.

[0021] Figure 2 This is a schematic diagram of the detection of antioxidant indicators in early pregnancy of sows according to the present invention; Figure 2 A is the serum T-AOC level of pregnant sows; Figure 2 B is the serum SOD level of pregnant sows; Figure 2 C is the serum CAT level of pregnant sows; Figure 2 D is the serum MDA level of pregnant sows.

[0022] Figure 3 is the alpha diversity of the sow feces flora of the present invention; wherein Figure 3 A is the alpha diversity of fecal flora of sows on day 0 (T1) of pregnancy; Figure 3 B is the alpha diversity of fecal flora of sows on the 16th day of pregnancy (T2).

[0023] Figure 4 is a relative abundance diagram of the genus level of the fecal flora of sows of the present invention; wherein Figure 4A is the relative abundance of fecal flora at the genus level (top 15) on day 0 (T1) of sow gestation; Figure 4 B is the relative abundance of fecal flora at the genus level (top 15) on the 16th day of gestation (T2) of sows.

[0024] Figure 5 This is a bar graph showing the distribution of LDA values ​​for LEfSe analysis of fecal flora screening of sows on the 16th day of pregnancy according to the present invention.

[0025] Figure 6 Representative fluorescent images of Hoechst 33342 blastocysts stained in the present invention.

[0026] Figure 7 It is the EdU positive cell rate of the blastocyst of the present invention.

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Embodiment 1:

[0029] 1. Experimental Materials and Methods

[0030] 1.1 Experimental animals: The sows were Dongliao black pigs, raised at the Agricultural Experimental Base of Jilin University.

[0031] 1.2 Main test reagents: see Table 1.

[0032] Table 1 Test reagents

[0033]

[0034] 1.3 Experimental method: 100 Dongliao black pigs with similar body weight and parity (2-3 parities) were randomly divided into 5 groups on the day of mating (gestation day 0), with 20 sows in each group, named control group (CON), treatment group 1 (T1), treatment group 2 (T2), treatment group 3 (T3) and treatment group 4 (T4). Sows in each group were fed with a basic diet + different levels of Eucommia ulmoides extract (see Table 2). After the sows gave birth, the total number of piglets born, the number of live piglets born alive, the live piglet birth weight and the live piglet birth weight were recorded.

[0035] The corresponding indicators are calculated according to the following formula:

[0036] Sow conception rate (%) = number of pregnant sows × 100% / total number of sows.

[0037] Stillbirth rate (%) = (1-number of live piglets born / total number of piglets born) × 100%.

[0038] Coefficient of variation of birth weight of live piglets within a litter (%) = (standard deviation of birth weight of live piglets / mean birth weight of live piglets) × 100%.

[0039] Table 2 Diet composition of pregnant sows

[0040]

[0041] 2. Experimental results:

[0042] Depend on Figure 1 As shown in the results, the total number of piglets born and the number of live piglets born in the T2 group were significantly higher than those in the CON group, and the stillbirth rate was significantly lower than that in the CON group; the live piglet birth weight in the T2 group and the live piglet birth weight in the T1, T2, and T3 groups were significantly higher than those in the CON group and the T4 group, but there were no significant differences in the live piglet birth weight and live piglet birth weight among the T1, T2, and T3 groups; compared with the control group, the total number of piglets born, the number of live piglets born, the live piglet birth weight and the stillbirth rate of the sows in the T1, T3, and T4 groups were not significantly different, but the total number of piglets born, the number of live piglets born and the live piglet birth weight showed an upward trend, and the stillbirth rate showed a downward trend. These results indicate that Eucommia ulmoides extract has a significant effect on improving the production performance of sows, and the feeding time and feeding dose of Eucommia ulmoides extract in the T2 group are the best, that is, the sows are supplemented with 2000 mg / kg of Eucommia ulmoides extract with a chlorogenic acid content of 50% daily from the 1st to the 15th day of pregnancy.

[0043] Embodiment 2:

[0044] 1. Experimental Materials and Methods

[0045] 1.1 Experimental animals: The sows were Dongliao black pigs, raised at the Agricultural Experimental Base of Jilin University.

[0046] 1.2 Main test reagents: see Table 3.

[0047] Table 3 Test reagents

[0048]

[0049] 1.3 Main test instruments: see Table 4.

[0050] Table 4 Test instruments

[0051]

[0052] 1.4 Experimental method: 40 Dongliao black pigs with similar body weight and parity (2-3 parities) were randomly divided into two groups on the day of mating (gestation day 0), with 20 sows in each group, named control group (CON) and Eucommia extract group (CGA). CON group sows: fed with basal diet from 1st to 15th day of pregnancy; CGA group sows: fed with basal diet + 2000mg / kg / day Eucommia extract from 1st to 15th day of pregnancy. On the 16th day of pregnancy, blood was collected from the anterior vena cava of the sows, and serum samples were collected after centrifugation at 4000rpm for 10 minutes. The MDA, SOD, CAT and T-AOC indicators of the sow serum samples were determined by ELISA kit according to the operating instructions.

[0053] 2. Test results:

[0054] like Figure 2 As shown in the results, Eucommia extract can significantly increase the serum T-AOC, SOD and CAT activities of sows in early pregnancy and reduce the serum MDA content of sows. These indicators are related to the antioxidant capacity of sows, indicating that Eucommia extract can significantly reduce the oxidative stress of sows in early pregnancy.

[0055] Embodiment 3:

[0056] 1. Experimental Materials and Methods

[0057] 1.1 Experimental animals: The sows were Dongliao black pigs, raised at the Agricultural Experimental Base of Jilin University.

[0058] 1.2 Main test reagents: see Table 5.

[0059] Table 5 Test reagents

[0060]

[0061] 1.3 Main test instruments: see Table 6.

[0062] Table 6 Test instruments

[0063]

[0064] 1.4 Experimental method: 16 Dongliao black pigs with similar body weight and parity (2-3 parities) were randomly divided into 2 groups on the day of mating (gestation day 0), with 8 sows in each group, named control group (CON) and Eucommia extract group (CGA). CON group sows: fed with basal diet from gestation day 1 to 15; CGA group sows: fed with basal diet + 2000mg / kg / day Eucommia extract from gestation day 1 to 15. Fresh fecal samples were collected from sows before feeding Eucommia extract (gestation day 0) and after feeding Eucommia extract (gestation day 16), respectively, and placed in 5mL sterile polypropylene tubes and frozen at -80℃ for subsequent DNA extraction.

[0065] 1.5 DNA extraction of sow fecal microbiome and high-throughput sequencing of bacterial 16S rDNA gene: Sow fecal samples were used Kit (100) was used for DNA extraction. DNA quality was assessed by 1.2% agarose gel electrophoresis, and the concentration was quantified by Nanodrop. The hypervariable variable regions V3 and V4 of the 16S rRNA gene were PCR amplified with primers 341F (5′-CCTAYGGGRBGCASCAG-3′) and 806R (5′-GACTACNNGGGTATCTAAT-3′). Finally, the amplicon library was sequenced on the Illumina MiSeq platform, and the generated 250 bp paired-end sequences were evaluated using QIME2 (version 2019.4). Primer sequences and low-quality reads (Q<25) were denoised using the DADA2 plug-in, high-quality paired-end clean reads were merged into tags using FLASH, and chimeras and monomers were then removed to obtain ASVs. The ASVs sequences were annotated and species classified based on the Greengenes2 database.

[0066] 1.6 Analysis of sow fecal sequencing data: Based on Chao1, Shannon, Observed-species, Faith-pd and Pielou-e indices, the alpha diversity of fecal flora was calculated and analyzed using □QIME2 (version 2019.4); species-level abundance differences between groups were analyzed; for the screening of specific flora, the LEfSe (LDA Effect Size) threshold was set to 4.0.

[0067] 2. Test results:

[0068] 2.1 Analysis of alpha diversity of intestinal flora

[0069] The results of bacterial alpha diversity analysis based on Chao1, Shannon, Observed species, Paith_pd and Pielou_e index showed that there was no significant difference in Chao1, Shannon, Observed species, Paith_pd and Pielou_e index of fecal flora between the CON group and the CGA group before feeding Eucommia extract. Figure 3 A). After the sows finished feeding Eucommia extract, the Chao1, Shannon, Observed species, Paith_pd and Pielou_e indexes of fecal flora in the CGA group were significantly higher than those in the CON group ( Figure 3B). This indicates that Eucommia ulmoides extract has the effect of regulating the alpha diversity of intestinal flora in the early stage of pregnant sows, and can significantly improve the richness and uniformity of fecal flora in pregnant sows.

[0070] 2.2 Analysis of differences in intestinal flora composition

[0071] In order to analyze whether there are significant differences in the types of fecal flora of sows between different groups, the top 15 species at the relative abundance level of the classification genus were selected for difference analysis. The results showed that before the sows were fed with Eucommia extract, the top 15 genera at the relative abundance level of the fecal flora were Terrisporobacter, Lactobacillus, Romboutsia_B, Clostridium_T, Turicibacter, Cryptobacteroides, Prevotella, Clostridium_D, Limosilactobacillus, RF16, Vescimonas, Faecousia, Treponema_F, Limivicinus, and Alloprevotella, but there were no significant differences in these 15 genera between the CON group and the CGA group, such as Figure 4 A. After sows were fed with Eucommia extract, the top 15 genera in relative abundance in fecal flora were Terrisporobacter, Clostridium_T, Turicibacter, Prevotella, Romboutsia_B, UBA4334, Treponema_D, Cryptobacteroides, Lactobacillus, Faecousia, Sodaliphilus, Limosilactobacillus, RF16, Streptococcus and SFM101. The dominant genera in the CON group were Turicibacter, Romboutsia_B, Terrisporobacter, Clostridium_T and Streptococcus, and their relative abundance was significantly higher than that in the CGA group. The dominant genera in the CGA group were UBA4334, Prevotella, Treponema_D, RF16, Cryptobacteroides and Sodaliphilus, and their relative abundance was significantly higher than that in the CON group. Figure 4 B.

[0072] 2.3 Screening of specific intestinal flora

[0073] In this study, 31 biomarkers were found to be significantly different between the two groups after sows were fed Eucommia extract. At the phylum level, the relative abundance of Bacteroidota, Spirochaetota, Firmicutes_A, and Firmicutes_D was significantly different between the two groups. The LDA score showed that Terrisporobacter, Turicibacter, Clostridium_T, Romboutsia_B, and Streptococcus dominated the CON group, while UBA4334, Treponema_D, Prevotella, Cryptobacteroides, and Sodaliphilus dominated the CGA group. Figure 5 .

[0074] Turicibacter has a negative impact on the healthy function of the intestine and the maintenance of serum metabolic index, and is associated with intestinal diseases. Terrisporobacter is an anaerobic pathogen that has been shown to induce oxidative stress. Romboutsia is a conditional pathogen associated with intestinal inflammation and chronic contractile damage. In addition, Streptococcus is the main pathogen of porcine endometritis and is associated with inflammation at the intestinal level. Most species of Clostridium are saprophytes, and a few species can produce highly toxic exotoxins and invasive enzymes, which are pathogenic to humans or animals. Treponema_D and Cryptobacteroides are taxa closely related to geography and lifestyle. UBA4334 belongs to Bacteroidaceae. Studies have shown that a significant increase in Bacteroidaceae can increase the content of short-chain fatty acids. Prevotella can use xylanases, mannanases, and β-glucanases to decompose polysaccharides in grain cell walls, participate in the fermentation of undigested carbohydrates, and thus produce short-chain fatty acids, which help increase the host's energy acquisition. Sodaliphilus is a newly discovered genus, and Sodaliphilus pleomorphus was reported to be isolated from pig feces for the first time, indicating that members of the genus Sodaliphilus are widespread in the pig intestine and may represent previously unreported bacterial taxa.

[0075] In summary, after sows were fed with Eucommia extract, the relative abundance of bacterial genera related to inflammation level and induced oxidative stress in fecal samples of sows in the CON group was significantly higher than that in fecal samples of sows in the CGA group, while the abundance of bacterial genera related to energy metabolism was significantly lower than that in fecal samples of sows in the CGA group. This may be related to the fact that Eucommia extract improves individual oxidative stress, digestive ability and disease resistance of sows in early pregnancy. Therefore, the changes in the abundance of the above bacterial genera can provide guidance for analyzing the health status and nutrient digestion and absorption ability of sows in early pregnancy.

[0076] Embodiment 4:

[0077] 1 Experimental materials and methods

[0078] 1.1 Experimental animals: The sows were Dongliao black pigs, raised at the Agricultural Experimental Base of Jilin University.

[0079] 1.2 Main test reagents: see Table 7.

[0080] Table 7 Test reagents

[0081]

[0082] 1.3 Main test instruments: see Table 8.

[0083] Table 8 Test instruments

[0084]

[0085] 1.4 Experimental method: 10 Dongliao black pigs with similar body weight and parity (2-3 parities) were randomly divided into 2 groups on the day of mating (gestation day 0), with 5 sows in each group, named control group (CON) and Eucommia extract group (CGA). CON group sows: fed with basal diet from 1 to 6 days of pregnancy; CGA group sows: fed with basal diet + 2000 mg / kg / day Eucommia extract from 1 to 6 days of pregnancy. On the 7th day of pregnancy, the sows were anesthetized by inhalation, and the oxygen flow and anesthetic adjustment are shown in Table 9. After the sows entered the anesthesia state, their abdomens were cut open, the uterus was removed, and the blastocysts in the uterine horns were collected for Hoechst 33342 staining and EdU cell proliferation detection.

[0086] Table 9 Oxygen flow and anesthetic regulation

[0087]

[0088] 1.5Hoechst 33342 staining: The porcine blastocysts were fixed with 4% paraformaldehyde (w / v) at room temperature for 30 minutes. Then, they were permeabilized with 0.1% Trition X-100 at room temperature for 30 minutes. Then, 10 μg / mL Hoechst33342 was used for staining for 10 minutes. Each step was washed 3 times with PBS-PVA solution, and then the blastocyst samples were sealed with anti-fluorescence quenching sealing agent. Finally, the total number of blastocyst cells photographed by fluorescence microscopy was counted using ImageJ software.

[0089] 1.6 EdU cell proliferation detection: using BeyoClick TM The EdU-555 cell proliferation detection kit was used to evaluate the cell proliferation of porcine blastocysts. After the blastocyst cells were incubated in the dark with 10μM EdU for 2 hours at 38.5°C and 5% CO2, the blastocyst cells were washed 3 times with PBS-PVA and fixed with 4% paraformaldehyde for 15 minutes. The blastocyst cells were permeabilized by treatment with 0.1% Triton X-100 and washed three times with PBS-PVA. Then, the blastocyst cells were stained in the dark with Azide Alexa Fluor 555 solution for 30 minutes, and the blastocysts were stained in the dark with Hoechst33342 for 15 minutes. Subsequently, the slides were washed 3 times with PBS-PVA and sealed. Finally, the EdU-positive cells in the blastocyst were counted using an inverted fluorescence microscope and ImageJ software. EdU-positive cell rate (%) = number of EdU-positive cells / total number of blastocyst cells × 100%.

[0090] 2. Test results:

[0091] The test results are as follows Figure 6 , Figure 7 As shown, the results showed that Eucommia ulmoides extract could significantly increase the EdU positive cell rate in blastocysts, indicating that Eucommia ulmoides extract could promote the proliferation of porcine blastocyst cells.

[0092] In summary, feeding Eucommia ulmoides extract in early pregnancy can significantly increase the total number of piglets born, the total number of live piglets, the birth weight of live piglets and the birth weight of live piglets, and reduce the stillbirth rate; it can also improve the antioxidant capacity and early embryo proliferation capacity of sows in early pregnancy.

[0093] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0094] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An application of feed for improving sow reproductive performance, characterized in that: The feed is a basic diet supplemented with an eucommia extract containing 50% chlorogenic acid.

2. The use of a feed for improving sow reproductive performance according to claim 1, characterized in that: The basic diet is a commercial diet.

3. The use of a feed for improving sow reproductive performance according to claim 1, characterized in that: The feed feeding stage is the early pregnancy of the sow, and the feeding amount of the eucommia extract is 600-2000 mg / kg / day.

4. The use of a feed for improving sow reproductive performance according to claim 3, characterized in that: The early stage of sow pregnancy is selected from the 1st to 15th day or the 16th to 30th day of pregnancy.

5. The use of a feed for improving sow reproductive performance according to claim 4, characterized in that: The early stage of sow pregnancy is selected from the 1st to 15th day of pregnancy.

6. The use of a feed for improving sow reproductive performance according to claim 3, characterized in that: The feeding amount of the Eucommia ulmoides extract is 2000 mg / kg / day.

7. The use of a feed for improving sow reproductive performance according to claim 1, characterized in that: The method comprises improving the production performance of sows and enhancing the antioxidant capacity of sows in early pregnancy. The improving the production performance of sows comprises increasing the total number of piglets born, the total number of piglets born alive, the live piglet birth weight, the live piglet birth litter weight, and reducing the stillbirth rate. The improving the antioxidant capacity of sows in early pregnancy comprises increasing the activities of T-AOC, SOD and CAT in the serum of sows and reducing the MDA content in the serum of sows.

8. The use of a feed for improving sow reproductive performance according to claim 1, characterized in that: The method comprises regulating the alpha diversity and composition of the intestinal flora of early pregnant sows, wherein the regulating the alpha diversity of the intestinal flora of early pregnant sows comprises increasing the Chao1, Shannon, Observed-species, Faith-pd and Pielou-e index of the fecal flora of sows; and the regulating the composition of the intestinal flora of early pregnant sows comprises reducing the relative abundance of Terrisporobacter, Turicibacter, Clostridium_T, Romboutsia_B and Streptococcus in the fecal flora of sows, and increasing the relative abundance of UBA4334, Treponema_D, Prevotella, Cryptobacteroides and Sodaliphilus in the fecal flora of sows.

9. The use of a feed for improving sow reproductive performance according to claim 1, characterized in that: The method comprises improving the quality of early embryonic development of sows, wherein the method comprises improving the EdU positive cell rate of blastocysts.

10. A feeding method for improving the reproductive performance of sows, characterized in that: The method comprises feeding the eucommia extract or feed containing 50% chlorogenic acid eucommia extract to the sow on the 1st to 15th day of pregnancy, wherein the feeding amount of the eucommia extract is 2000 mg / kg / day.

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