Self-repairing composition suitable for sensitive skin as well as preparation and application of self-repairing composition

Abstract

The invention relates to the technical field of skin care, and discloses a self-repairing composition suitable for sensitive skin and preparation and application of the self-repairing composition. The self-repairing composition is formed by compounding lucid ganoderma source nanofibers and repairing liquid according to the mass ratio of 1: (50-60); wherein the lucid ganoderma source nanofiber is prepared from a lucid ganoderma source chitosan spinning solution through an electrostatic spinning process; according to the self-repairing composition prepared by the invention, from the formation mechanism of sensitive skin, the sensitive skin is repaired in three aspects of barrier repairing, inflammatory response blocking and nerve high response inhibiting; the sensitive state of sensitive people can be improved, and the skin tolerance degree is improved. The repairing capability of the machine body is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and in particular to a self-repairing composition suitable for sensitive skin and a preparation method and application thereof. Background Art

[0002] Sensitive skin (SS) refers to a highly reactive state of the skin under physiological or pathological conditions, mainly occurring on the face. Clinically, the skin is prone to subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, mental and other factors, with or without objective signs such as erythema, scaling, and capillary dilation. Due to the complexity of the causes of sensitive skin, there is no definite standard treatment method. There is an urgent need to find an efficient and safe method to treat sensitive skin.

[0003] It is generally believed that the occurrence of sensitive skin is a complex process involving skin barrier, nerve blood vessels, and immune inflammation. Currently, the products commonly used on the market for sensitive skin mostly use the functions of soothing skin inflammation or repairing skin barrier as the product function. Few of them take into account the three aspects of barrier damage, inflammatory response, and high nerve response, and the effect is not ideal.

[0004] The formation mechanism of sensitive skin includes: barrier damage, inflammatory response, and neural hyperresponsiveness; among them, barrier damage includes: incomplete sebum membrane: the sebum membrane of sensitive skin is often incomplete and cannot keep the skin hydrated well; damaged stratum corneum: the keratinization process of sensitive skin is abnormal. Based on the formation process of the stratum corneum, the health of the barrier structure can be enhanced from five aspects: filaggrin FLG metabolism, the formation of keratinized envelope CE, the formation of intercellular lipids, corneous desmosomes, and tight junctions. From an anatomical point of view, compared with adult skin, the stratum corneum and epidermis of infant skin are thinner, the keratinocytes are smaller, the transepidermal water loss (TEWL) is higher, the pH value is higher, and the barrier function is immature, which belongs to the category of sensitive skin; inflammatory response includes: there are multiple inflammatory pathways in the human body, and complex physiological reactions occur. The same inflammatory factor can activate different pathways, different inflammatory pathways can produce the same inflammatory factor, and different inflammatory pathways can affect each other; neural hyperresponsiveness: transient receptor potential vanilloid subtype 1 channel protein (TRPV1), which is highly expressed in sensitive skin populations. TRPV1, as a classic neuronal receptor, is not only expressed on nerve endings, but also widely expressed in skin cells and immune cells. The cell type that expresses TRPV1 most abundantly in the epidermis is keratinocytes. In sensitive skin, more TRPV1 nerve receptors are activated, increasing calcium ion flow and causing calcium imbalance, which can significantly aggravate skin irritation and inflammatory reactions. Blocking TRPV1 can reduce skin sensitivity and relieve skin sensitivity.

[0005] Therefore, based on the formation mechanism of sensitive skin, sensitive skin care can be carried out from three aspects: barrier repair, blocking inflammatory response, and inhibiting high nerve response.

[0006] Based on this, we propose a self-repair composition suitable for sensitive skin. Summary of the invention

[0007] 1. Technical issues to be resolved In view of the deficiencies of the prior art, the present invention provides a self-repair composition suitable for sensitive skin and a preparation method thereof.

[0008] (II) Technical solution To achieve the above-mentioned purpose, the present invention provides the following technical solutions: A self-repairing composition suitable for sensitive skin, which is composed of Ganoderma lucidum-derived nanofibers and repair liquid in a mass ratio of 1:50-60; Among them, the Ganoderma lucidum nanofiber is made by using Ganoderma lucidum chitosan spinning solution through an electrospinning process; The repair liquid is made of the following ingredients by weight: 1-2 parts of butylene glycol, 1.2-2 parts of squalane, 0.4-0.5 parts of kava leaf / root / stem extract, 0.15-0.3 parts of hyssop extract, 0.12-0.2 parts of 1,2-pentanediol, 0.015-0.018 parts of 1,2-hexanediol, 0.5-0.7 parts of p-hydroxyacetophenone, 0.2-0.4 parts of polyglutamic acid, 0.32-0.36 parts of tetrahydromethylpyrimidine carboxylic acid, 0.1-0.3 parts of hydrolyzed sclerotium gum, 0.001-0.002 parts of 4-tert-butyl cyclohexanol, 0.00012-0.00015 parts of cherimoya fruit extract, 0.08-0.010 parts of sodium hyaluronate, 1.2-1.5 parts of trehalose, and 70-75 parts of water.

[0009] As a further technical solution, the ganoderma lucidum-derived nanofibers are made from ganoderma lucidum-derived chitosan spinning solution through an electrostatic spinning process; The method for preparing the Ganoderma lucidum chitosan spinning solution is as follows: Firstly, the ganoderma lucidum spore powder is prepared into a suspension, and then subjected to a cell wall breaking treatment, and then filtered and dried to obtain a cell wall breaking material; The defatted material is treated with sodium hydroxide solution, centrifuged, filtered and washed until neutral to obtain an intermediate material; Pre-treating the intermediate material to obtain pre-treated material; Extracting the pretreated material to obtain Ganoderma lucidum source chitosan; The ganoderma lucidum-derived chitosan is added into an acetic acid solution to obtain a spinning solution; The mixing ratio of Ganoderma lucidum chitosan and acetic acid solution is 12-15 g: 100 mL; The mass fraction of the acetic acid solution is 1%; Spinning process parameters: the spinneret model is 20G, the receiving distance is 12cm, the spinning solution propulsion speed is 0.5mL / h, the voltage is 18kV, and the prepared spinning solution is used for electrostatic spinning, and dried at 60℃ for 2h to obtain Ganoderma lucidum source nanofibers.

[0010] As a further technical solution, the concentration of Ganoderma lucidum spore powder in the suspension is 0.08 g / mL; The cell wall was broken by high-voltage pulse electric field; the specific parameters were: electric field intensity 22 kV / cm, number of pulses 35,000, pulse width 80 μs, and suspension processing volume 100 mL.

[0011] As a further technical solution: degreasing the broken material to obtain a degreased material; The degreasing treatment is as follows: take the broken material, add petroleum ether in a ratio of 1:30 (g / mL), stir at a speed of 500r / min for 25 minutes, let it stand for 1 hour, then filter it, continue to add petroleum ether in a ratio of 1:25 (g / mL), repeat once, and finally filter it, and dry it in an oven at 60°C for 4 hours to obtain it.

[0012] As a further technical solution: the alkaline solution treatment is as follows: the defatted material is mixed with a sodium hydroxide solution, the temperature is adjusted to 80°C, the mixture is stirred in a water bath for 5 hours, and then centrifuged for 10 minutes, filtered, washed with water until neutral, and dried at 60°C for 10 hours; The mixing ratio of the degreasing material and the sodium hydroxide solution is 10g:150mL; The mass fraction of the sodium hydroxide solution is 5%; The centrifugal speed is 8500r / min.

[0013] As a further technical solution: the pretreatment is: The intermediate material was mixed with hydrogen peroxide, the temperature was adjusted to 75°C, the mixture was stirred for 3 hours, the pH of the reaction system was adjusted to neutral, the mixture was centrifuged and dried to obtain the pretreated material; The mixing ratio of the intermediate material and hydrogen peroxide is 10g:120mL; The water quality score of both eyes is 25%; The drying temperature was 60°C and the drying time was 5 hours.

[0014] As a further technical solution: the extraction is as follows: the pretreated material and the sodium hydroxide solution are mixed in a mass ratio of 1:10, and then the temperature is adjusted to 80°C, and the mixture is stirred for 12 hours at the temperature, and then centrifuged for 10 minutes, and the precipitate is collected, washed with water until neutral, and dried to obtain the product; The mass fraction of the sodium hydroxide solution is 10%; The centrifugal speed is 9000r / min; The drying temperature is 65°C.

[0015] As a further technical solution: the Piper methysticum leaf / root / stem extract, Hyssop extract, and Annona mume fruit extract are all obtained by mixed extraction with 80% ethanol solution and then dried.

[0016] A method for preparing a self-repairing composition suitable for sensitive skin comprises the following steps: (1) Weigh the raw materials according to the proportion of each component; (2) Ganoderma lucidum-derived chitosan spinning solution was used to prepare Ganoderma lucidum-derived nanofibers through an electrospinning process; (3) Butylene glycol, squalane, kava leaf / root / stem extract, hyssop extract, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, polyglutamic acid, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sclerotium gum, 4-tert-butyl cyclohexanol, Annona mume fruit extract, sodium hyaluronate, trehalose, and water are mixed together in sequence and stirred for 20 minutes to obtain a repair solution; (4) Ganoderma lucidum-derived nanofibers are added to the repair solution, stirred and mixed evenly, and then ultrasonically dispersed for 10 minutes to obtain.

[0017] The self-repairing composition prepared by the method is applied to repair sensitive skin.

[0018] (III) Beneficial effects Compared with the prior art, the present invention provides a self-repair composition suitable for sensitive skin, which has the following beneficial effects: The self-repairing composition prepared by the present invention repairs sensitive skin from three aspects: barrier repair, blocking inflammatory response, and inhibiting high nerve response, starting from the formation mechanism of sensitive skin; It can improve the sensitivity of sensitive people, increase skin tolerance, and enhance the body's repair ability.

[0019] By introducing Ganoderma lucidum nanofibers, it can play the following roles: ①Immune regulation: activate macrophages, regulate skin immunity, and maintain skin homeostasis; ②Promote repair: regulate inflammatory response, reduce the expression of inflammatory factors IL-6 and TNF-ɑ; promote cell release of growth factors and cell tissue proliferation; ③Moisturizing: Improve skin elasticity and skin texture; By introducing tetrahydromethylpyrimidine carboxylic acid, it can play the role of: ① Barrier repair: Increase the content of FLG and LOR in the skin, increase the content of fibroblasts, thicken the epidermis, and repair damage; ② Anti-inflammatory: It can inhibit the secretion of pro-inflammatory factors IL-1α and TNF-α and exert anti-inflammatory effects; ③ Anti-irritation: protect the skin from damage by ultraviolet radiation, pollution particles, allergens, cold, heat, dryness or chemical stress factors; Through the combined effects of cherimoya fruit extract and kava extract, the release of inflammatory factors mediated by TRPV1 and the expression of CGRP mediated by histamine can be reduced; through the rich capsaicin, triple nerve soothing can be achieved, instantly relieving discomfort and reducing redness; Through the combination of 4-tert-butyl cyclohexanol and Hyssopus officinalis extract: it can reduce skin burning sensation, resist irritation and enhance skin tolerance; through the rich flavonoids and steroidal components, it can inhibit the release of inflammatory factors IL-1β, TNF-α and PGE2, hinder the NF-κB signaling pathway and arachidonic acid signaling pathway, regulate the inflammatory cascade reaction, soothe skin sensitivity, and have a significant soothing effect. Specific embodiments Figure 1 This is the three-dimensional thermal image of the first randomly selected subject; Figure 2 This is the three-dimensional thermal image of the 10th randomly selected subject; Figure 3 This is the change diagram of the red area on the face of the second subject; Figure 4 This is a graph showing the changes in the red area on the facial skin of the fifth subject. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0022] Example 1 A self-repairing composition suitable for sensitive skin, which is composed of Ganoderma lucidum-derived nanofibers and repair liquid in a mass ratio of 1:50; Among them, the Ganoderma lucidum nanofiber is made by using Ganoderma lucidum chitosan spinning solution through an electrospinning process; The repair liquid is made of the following ingredients by weight: 1 part of butylene glycol, 1.2 parts of squalane, 0.4 parts of kava leaf / root / stem extract, 0.15 parts of hyssop extract, 0.12 parts of 1,2-pentanediol, 0.015 parts of 1,2-hexanediol, 0.5 parts of p-hydroxyacetophenone, 0.2 parts of polyglutamic acid, 0.32 parts of tetrahydromethylpyrimidine carboxylic acid, 0.1 parts of hydrolyzed sclerotium gum, 0.001 parts of 4-tert-butyl cyclohexanol, 0.00012 parts of cherimoya fruit extract, 0.08 parts of sodium hyaluronate, 1.2 parts of trehalose, and 70 parts of water.

[0023] The ganoderma lucidum nanofiber is made from ganoderma lucidum chitosan spinning solution through an electrostatic spinning process; The method for preparing the Ganoderma lucidum chitosan spinning solution is as follows: Firstly, the ganoderma lucidum spore powder is prepared into a suspension, and then subjected to a cell wall breaking treatment, and then filtered and dried to obtain a cell wall breaking material; The defatted material is treated with sodium hydroxide solution, centrifuged, filtered and washed until neutral to obtain an intermediate material; Pre-treating the intermediate material to obtain pre-treated material; Extracting the pretreated material to obtain Ganoderma lucidum source chitosan; The ganoderma lucidum-derived chitosan is added into an acetic acid solution to obtain a spinning solution; The mixing ratio of Ganoderma lucidum chitosan and acetic acid solution is 12 g:100 mL; The mass fraction of the acetic acid solution is 1%; Spinning process parameters: the spinneret model is 20G, the receiving distance is 12cm, the spinning solution propulsion speed is 0.5mL / h, the voltage is 18kV, and the prepared spinning solution is used for electrostatic spinning, and dried at 60℃ for 2h to obtain Ganoderma lucidum source nanofibers.

[0024] Among them, the concentration of Ganoderma lucidum spore powder in the suspension is 0.08g / mL; The cell wall was broken by high-voltage pulse electric field; the specific parameters were: electric field intensity 22 kV / cm, number of pulses 35,000, pulse width 80 μs, and suspension processing volume 100 mL.

[0025] Degreasing the broken material to obtain a degreased material; The degreasing treatment is as follows: take the broken material, add petroleum ether in a ratio of 1:30 (g / mL), stir at a speed of 500r / min for 25 minutes, let it stand for 1 hour, then filter it, continue to add petroleum ether in a ratio of 1:25 (g / mL), repeat once, and finally filter it, and dry it in an oven at 60°C for 4 hours to obtain it.

[0026] The alkaline solution treatment is as follows: the defatted material is mixed with a sodium hydroxide solution, the temperature is adjusted to 80°C, the mixture is stirred in a water bath for 5 hours, and then centrifuged for 10 minutes, filtered, washed with water until neutral, and dried at 60°C for 10 hours; The mixing ratio of the degreasing material and the sodium hydroxide solution is 10g:150mL; The mass fraction of the sodium hydroxide solution is 5%; The centrifugal speed is 8500r / min.

[0027] The preprocessing is: The intermediate material was mixed with hydrogen peroxide, the temperature was adjusted to 75°C, the mixture was stirred for 3 hours, the pH of the reaction system was adjusted to neutral, the mixture was centrifuged and dried to obtain the pretreated material; The mixing ratio of the intermediate material and hydrogen peroxide is 10g:120mL; The water quality score of both eyes is 25%; The drying temperature was 60°C and the drying time was 5 hours.

[0028] The extraction is as follows: the pretreated material and the sodium hydroxide solution are mixed in a mass ratio of 1:10, and then the temperature is adjusted to 80°C, and the mixture is stirred for 12 hours at the temperature, and then centrifuged for 10 minutes, and the precipitate is collected, washed with water until neutral, and dried to obtain the product; The mass fraction of the sodium hydroxide solution is 10%; The centrifugal speed is 9000r / min; The drying temperature is 65°C.

[0029] Kava leaf / root / stem extract, Hyssop extract, and Annona mume fruit extract are all obtained by mixed extraction with 80% ethanol solution and drying.

[0030] The specific extraction method is as follows: after the raw material is crushed, it is mixed with 80% ethanol solution at a mass ratio of 1:10 for 1 hour, and then filtered to obtain a filtrate and a filter residue, the filter residue is subjected to the above operation for 3 times, and the filtrates are combined for multiple times, and then spray dried to obtain; Kava leaf / root / stem extract is extracted from the root, Hyssop extract is extracted from the leaf, and Annona mume fruit extract is extracted from Annona mume fruit; A method for preparing a self-repairing composition suitable for sensitive skin comprises the following steps: (1) Weigh the raw materials according to the proportion of each component; (2) Ganoderma lucidum-derived chitosan spinning solution was used to prepare Ganoderma lucidum-derived nanofibers through an electrospinning process; (3) Butylene glycol, squalane, kava leaf / root / stem extract, hyssop extract, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, polyglutamic acid, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sclerotium gum, 4-tert-butyl cyclohexanol, Annona mume fruit extract, sodium hyaluronate, trehalose, and water are mixed together in sequence and stirred for 20 minutes to obtain a repair solution; (4) Ganoderma lucidum-derived nanofibers are added to the repair solution, stirred and mixed evenly, and then ultrasonically dispersed for 10 minutes to obtain.

[0031] Example 2 On the basis of the technical scheme of Example 1, the difference from the technical scheme of Example 1 is that the repair liquid includes the following ingredients by weight: 1.5 parts of butylene glycol, 1.8 parts of squalane, 0.45 parts of kava pepper leaf / root / stem extract, 0.18 parts of hyssop extract, 0.16 parts of 1,2-pentanediol, 0.017 parts of 1,2-hexanediol, 0.6 parts of p-hydroxyacetophenone, 0.3 parts of polyglutamic acid, 0.33 parts of tetrahydromethylpyrimidine carboxylic acid, 0.2 parts of hydrolyzed sclerotium gum, 0.0015 parts of 4-tert-butyl cyclohexanol, 0.00014 parts of cherimoya fruit extract, 0.09 parts of sodium hyaluronate, 1.3 parts of trehalose, and 72 parts of water.

[0032] Example 3 On the basis of the technical scheme of Example 1, the difference from the technical scheme of Example 1 is that the repair liquid includes the following ingredients by weight: 2 parts of butylene glycol, 2 parts of squalane, 0.5 parts of kava pepper leaf / root / stem extract, 0.3 parts of hyssop extract, 0.2 parts of 1,2-pentanediol, 0.018 parts of 1,2-hexanediol, 0.7 parts of p-hydroxyacetophenone, 0.4 parts of polyglutamic acid, 0.36 parts of tetrahydromethylpyrimidine carboxylic acid, 0.3 parts of hydrolyzed sclerotium gum, 0.002 parts of 4-tert-butyl cyclohexanol, 0.00015 parts of cherimoya fruit extract, 0.010 parts of sodium hyaluronate, 1.5 parts of trehalose, and 75 parts of water.

[0033] The following is a comparative example: Comparative Example 1: On the basis of Example 1, no Ganoderma lucidum nanofibers are added, and the rest of the technical solutions are consistent with those of Example 1.

[0034] Comparative Example 2: On the basis of Example 1, the Ganoderma lucidum-derived nanofibers were replaced with chitosan, and the rest of the technical solutions were consistent with those of Example 1.

[0035] test: Safety evaluation of human trials 150 subjects (aged 30-50 years old, half male and half female) were divided into 5 groups, and the test products of the embodiments and comparative examples were directly used. The skin reaction was recorded according to the skin reaction grading standard, with 0 being "no reaction", 1 being "faint erythema", 2 being "erythema, infiltration, papule", 3 being "erythema, edema, papule, blister", and 4 being "erythema, edema, bulla".

[0036] Table 1

[0037] Adverse reactions: After 28 days of continuous use, 0 cases of skin adverse reactions were observed among 30 subjects with sensitive skin; As can be seen from Table 1, the examples and comparative examples are safe.

[0038] Skin barrier function 150 subjects (aged 30-50 years old, half male and half female) were divided into 5 groups, and the test products of the embodiments and comparative examples were directly used, with a dosage of 0.7g±0.5g each time, once in the morning and evening, and evenly applied on the face. The skin barrier function of the subjects was tested before treatment and 28 days after treatment, and the moisture content of the stratum corneum of the skin (CorneometerCM825 tester) and the transepidermal water loss value of the skin (Vapometer tester) were tested respectively.

[0039] Skin sensitivity test The skin sensitivity of the subjects was tested before and 28 days after treatment. The skin sensitivity parameters were tested (Tivi700 tester). The concentration of red blood cells was measured by using the polarized light spectral imaging principle and the wavelength difference absorbed by red blood cells and surrounding skin tissue. The higher the value of the skin sensitivity parameter, the more sensitive the skin.

[0040] Table 2

[0041] As can be seen from Table 2, the lower the value of the transepidermal water loss value, the better the skin barrier function. The subjects used the product of Example 1 for 28 consecutive days, and the moisture content of the stratum corneum of the skin increased significantly compared with the basal value (P<0.001), with an increase rate of 43.92%. The transepidermal water loss value of the skin decreased significantly compared with the basal value (P<0.001), with a decrease rate of 13.59%, and the effect was obvious.

[0042] Skin sensitivity parameters: When the subjects used the product of Example 1 for 28 consecutive days, the skin sensitivity parameters decreased significantly compared with the baseline values ​​(P<0.001), with a decrease rate of 6.51%.

[0043] Two subjects were randomly selected from the Example 1 group to compare the three-dimensional thermal images before and after 28 days of use. It can be clearly observed that the skin has improved. Figure 1 For the first randomly selected subject, Figure 2 This is the 10th subject randomly selected.

[0044] The test was carried out using Example 1 as the sample, and a blank control group using clean water was set up: Erythema Index ① The same researcher used VISIA facial image analyzer to collect data before and 28 days after treatment, and analyzed the skin redness a* value and skin red area ratio. The lower the VISIA skin redness a* value, the milder the skin redness. The lower the VISIA skin red area ratio, the smaller the skin redness area.

[0045] ② Perform tearing test on the inner side of the left and right arms of the subjects, and use Antera3D skin imaging measurement instrument to collect images and analyze data. The lower the average value of Antera3D skin redness, the lighter the skin redness; Table 3

[0046] Table 4

[0047] After the subjects used the test product for 28 consecutive days, the VISIA skin redness and a* values ​​decreased significantly compared with the baseline value (P<0.001), with a decrease rate of 8.14%. The VISIA skin red area ratio decreased significantly compared with the baseline value (P<0.001), with a decrease rate of 8.31%, as shown in Table 3.

[0048] Immediately after the subjects used the product of Example 1 once, the average value of Antera3D skin redness in the test product area was significantly decreased compared with that after tearing (0.01≤P<0.05), and the decrease rate was 2.42%; the average value of Antera3D skin redness in the blank control area was not significantly changed compared with that after tearing (P≥0.05), see Table 4.

[0049] Two subjects were randomly selected to compare the facial skin red zone change charts. The second and fifth subjects were randomly selected. The second subject was Figure 3 , the fifth subject is Figure 4 .

[0050] Subjective / semi-subjective evaluation ① Lactic acid sting test 50 μL of 10% lactic acid aqueous solution and control group-distilled water were dropped onto a medical filter with a diameter of 0.8 cm and applied to both sides of the nose. The subjects were asked about the skin stinging at 0.5 minutes, 2.5 minutes, and 5 minutes, and the pain was scored using a 4-point scale (0 for "no feeling", 1 for "mild feeling", 2 for "moderate feeling", and 3 for "severe feeling").

[0051] ② The sensitivity index was evaluated after modeling (Tafter modeling) and immediately after using the test product (Timmediately). The subjects were asked to evaluate the common subjective symptoms of sensitive facial skin (burning, stinging, itching), with 1 point for "strongly disagree", 2 points for "disagree", 3 points for "average", 4 points for "agree", and 5 points for "strongly agree".

[0052] Table 5

[0053] Subjective / semi-subjective evaluation showed that immediately after a single use of the product of the embodiment, the lactic acid stinging score was significantly decreased compared with that after modeling (P<0.001), with a decrease rate of 78.43%; the itching score was significantly decreased compared with that after modeling (P<0.001), with a decrease rate of 75.61%; the stinging score was significantly decreased compared with that after modeling (P<0.001), with a decrease rate of 82.31%; the burning score was significantly decreased compared with that after modeling (P<0.001), with a decrease rate of 77.67%.

[0054] The results of this test show that the use of the product of the present invention has an improvement effect on sensitive skin. The analysis is that the skin of the subjects with sensitive skin is already in a high-reaction state, and the skin barrier function is weaker than that of people with normal skin, making it more difficult to resist external stimuli. The water loss rate of sensitive skin through the epidermis increases, and the water content of the stratum corneum decreases, indicating that the barrier function of sensitive skin is impaired. The subjects tried the product for 28 consecutive days, and the transepidermal water loss value of the skin decreased significantly compared with the baseline value (P<0.001), and the water content of the stratum corneum of the skin increased significantly compared with the baseline value (P<0.001), indicating that the product of the present invention improves the skin barrier function. The reason is that Ganoderma lucidum nanofibers, as a biomaterial for wound repair, have good biocompatibility, no cytotoxicity, no irritation, can form a flexible and transparent film, effectively form a closed environment to repair the skin barrier, and then inhibit inflammatory reactions, thereby improving the allergic symptoms of the subjects.

[0055] The results of this test also showed that the erythema value of the subjects who used the product of the present invention for 28 consecutive days decreased significantly (P<0.001), and the average value of skin redness after physical stimulation and tearing decreased significantly compared with the blank control group (0.01≤P<0.05), indicating that the product of the present invention can relieve the redness symptoms of subjects with sensitive skin. Analysis shows that the protection of skin nerve endings is reduced after the skin barrier is damaged. High temperature, acidic environment, lactic acid, ultraviolet rays, and endogenous inflammatory mediators activate TRPV-1, thermal and chemical stimulation sensory input, causing symptoms such as burning, paroxysmal redness, pain, and itching. TRPV-1 induces mast cell degranulation and promotes the release of neurotransmitters, such as vasoactive substances intestinal polypeptide (VIP), substance P (SP), and calcitonin gene-related polypeptide (CGRP), triggering the occurrence of neurogenic inflammation. The ganoderma-derived nanofibers introduced in the present invention can partially inhibit the secretion of interleukin-8 (IL-8) and TNF-α in mast cells, indicating that the ganoderma-derived nanofibers have the effect of reducing allergic inflammatory reactions and alleviating the problem of skin redness. In addition, the expression of TRPV1 is significantly increased in people with high lactic acid stinging scores (sensitive skin). The results of this test show that immediately after the subjects used the test product once, the lactic acid stinging score decreased significantly compared with the modeling (P<0.001), indicating that the invented product reduces skin sensitivity and alleviates the skin sensitivity state. Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A self-repair composition suitable for sensitive skin, characterized in that: It is made of Ganoderma lucidum nanofiber and repair liquid in a mass ratio of 1:50-60; Among them, the Ganoderma lucidum nanofiber is made by using Ganoderma lucidum chitosan spinning solution through an electrospinning process; The repair liquid is made of the following ingredients by weight: 1-2 parts of butylene glycol, 1.2-2 parts of squalane, 0.4-0.5 parts of kava leaf / root / stem extract, 0.15-0.3 parts of hyssop extract, 0.12-0.2 parts of 1,2-pentanediol, 0.015-0.018 parts of 1,2-hexanediol, 0.5-0.7 parts of p-hydroxyacetophenone, 0.2-0.4 parts of polyglutamic acid, 0.32-0.36 parts of tetrahydromethylpyrimidine carboxylic acid, 0.1-0.3 parts of hydrolyzed sclerotium gum, 0.001-0.002 parts of 4-tert-butyl cyclohexanol, 0.00012-0.00015 parts of cherimoya fruit extract, 0.08-0.010 parts of sodium hyaluronate, 1.2-1.5 parts of trehalose, and 70-75 parts of water.

2. A self-repair composition suitable for sensitive skin according to claim 1, characterized in that: The ganoderma lucidum nanofiber is made from ganoderma lucidum chitosan spinning solution through an electrostatic spinning process; The method for preparing the Ganoderma lucidum chitosan spinning solution is as follows: Firstly, the ganoderma lucidum spore powder is prepared into a suspension, and then subjected to a cell wall breaking treatment, and then filtered and dried to obtain a cell wall breaking material; The defatted material is treated with sodium hydroxide solution, centrifuged, filtered and washed until neutral to obtain an intermediate material; Pre-treating the intermediate material to obtain pre-treated material; Extracting the pretreated material to obtain Ganoderma lucidum source chitosan; The ganoderma lucidum-derived chitosan is added into an acetic acid solution to obtain a spinning solution; The mixing ratio of Ganoderma lucidum chitosan and acetic acid solution is 12-15 g: 100 mL; The mass fraction of the acetic acid solution is 1%; Spinning process parameters: the spinneret model is 20G, the receiving distance is 12cm, the spinning solution propulsion speed is 0.5mL / h, the voltage is 18kV, and the prepared spinning solution is used for electrostatic spinning, and dried at 60℃ for 2h to obtain Ganoderma lucidum source nanofibers.

3. A self-repair composition suitable for sensitive skin according to claim 2, characterized in that: in, The concentration of Ganoderma lucidum spore powder in the suspension was 0.08 g / mL; The cell wall was broken by high-voltage pulse electric field; the specific parameters were: electric field intensity 22 kV / cm, number of pulses 35,000, pulse width 80 μs, and suspension processing volume 100 mL.

4. A self-repair composition suitable for sensitive skin according to claim 2, characterized in that: Degreasing the broken material to obtain a degreased material; The degreasing treatment is as follows: take the broken material, add petroleum ether in a ratio of 1:30 (g / mL), stir at a speed of 500r / min for 25 minutes, let it stand for 1 hour, then filter it, continue to add petroleum ether in a ratio of 1:25 (g / mL), repeat once, and finally filter it, and dry it in an oven at 60°C for 4 hours to obtain it.

5. A self-repairing composition suitable for sensitive skin according to claim 2, characterized in that: The alkaline solution treatment is as follows: the defatted material is mixed with a sodium hydroxide solution, the temperature is adjusted to 80°C, the mixture is stirred in a water bath for 5 hours, and then centrifuged for 10 minutes, filtered, washed with water until neutral, and dried at 60°C for 10 hours; The mixing ratio of the degreasing material and the sodium hydroxide solution is 10g:150mL; The mass fraction of the sodium hydroxide solution is 5%; The centrifugal speed is 8500r / min.

6. A self-repair composition suitable for sensitive skin according to claim 2, characterized in that: The pre-processing is: The intermediate material was mixed with hydrogen peroxide, the temperature was adjusted to 75°C, the mixture was stirred for 3 hours, the pH of the reaction system was adjusted to neutral, the mixture was centrifuged and dried to obtain the pretreated material; The mixing ratio of the intermediate material and hydrogen peroxide is 10g:120mL; The water quality score of both eyes is 25%; The drying temperature was 60°C and the drying time was 5 hours.

7. A self-repairing composition suitable for sensitive skin according to claim 2, characterized in that: The extraction is as follows: the pretreated material and the sodium hydroxide solution are mixed in a mass ratio of 1:10, and then the temperature is adjusted to 80°C, and the mixture is stirred for 12 hours at the temperature, and then centrifuged for 10 minutes, and the precipitate is collected, washed with water until neutral, and dried to obtain the product; The mass fraction of the sodium hydroxide solution is 10%; The centrifugal speed is 9000r / min; The drying temperature is 65°C.

8. The self-repair composition suitable for sensitive skin according to claim 1, characterized in that: The Piper methysticum leaf / root / stem extract, Hyssop extract and Annona chebula fruit extract are all obtained by mixed extraction with 80% ethanol solution and then drying.

9. The method for preparing a self-repairing composition suitable for sensitive skin according to claim 1, characterized in that: The following steps are involved: (1) Weigh the raw materials according to the proportion of each component; (2) Ganoderma lucidum-derived chitosan spinning solution was used to prepare Ganoderma lucidum-derived nanofibers through an electrospinning process; (3) Butylene glycol, squalane, kava leaf / root / stem extract, hyssop extract, 1,2-pentanediol, 1,2-hexanediol, p-hydroxyacetophenone, polyglutamic acid, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sclerotium gum, 4-tert-butyl cyclohexanol, Annona mume fruit extract, sodium hyaluronate, trehalose, and water are mixed together in sequence and stirred for 20 minutes to obtain a repair solution; (4) Ganoderma lucidum-derived nanofibers are added to the repair solution, stirred and mixed evenly, and then ultrasonically dispersed for 10 minutes to obtain.

10. The self-repairing composition prepared according to the method of claim 9 is used for repairing sensitive skin.

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