Application of jasminum crassifolium and extract of jasminum crassifolium in preparation of medicine for treating skin itch

Abstract

The invention discloses application of jasminum laurifolium and an extract of the jasminum laurifolium in preparation of a medicine for treating skin itch, and belongs to the technical field of traditional Chinese medicines. The jasminum laurifolium is subjected to water extraction to obtain a water extract of the jasminum laurifolium, or the jasminum laurifolium is extracted by adopting an organic solvent to obtain an organic solvent extract of the jasminum laurifolium, and the extraction method comprises the following steps: taking the jasminum laurifolium, adding the solvent for soaking treatment, heating for extraction, combining liquid medicines extracted for multiple times, and concentrating to obtain the jasminum laurifolium extract. It is found for the first time that the jasminum crassifolium and the extract thereof have the patent medicine potential for treating the skin itch, the jasminum crassifolium and the extract thereof can be used for preparing the medicine for treating the skin itch, the preparation method of the medicine preparation is simple, the cost is low, and the economic benefit of the patent medicine is good.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, in particular to application of Jasmine crassifolia and an extract thereof in preparing a medicine for treating skin itching. Background Art

[0002] Pruritus is a chronic skin disease without primary skin damage. It can be caused by certain diseases, drugs, cold, wool allergy and other stimuli. It is a neurological dysfunction skin disease and is still a difficult disease to treat. Pruritus manifests as local or systemic skin itching. Scratching causes secondary damage to the skin, such as scratches, blood scabs, and lichenification, which makes patients suffer. In recent years, the prevalence of pruritus has shown a significant upward trend, seriously affecting people's daily life and physical and mental health. At present, the pathogenesis of pruritus is still not very clear, so the clinical treatment of pruritus is difficult and there is no specific method. The most commonly used drugs are H1 receptor blockers, such as chlorpheniramine maleate tablets, ebastine tablets, and desloratadine citrate tablets. The itching is often relieved after taking the medicine, and the itching is aggravated after stopping the medicine. After taking the medicine, symptoms such as drowsiness, fatigue, and inattention often occur. Antibiotics and hormones can cause dysbacteriosis. It can be seen that the side effects of immunosuppressants are relatively large.

[0003] Traditional Chinese medicine has a long history of treating skin pruritus. It has the characteristics of multi-target, multi-link, multi-pathway, integrated regulation, and low toxicity and side effects. According to literature reports, Yangxue Rongfu Decoction can treat senile skin pruritus and improve patients' skin symptoms and TCM syndrome symptoms. Taohong Danggui Yinzi can effectively relieve the skin pruritus symptoms of hemodialysis patients with blood deficiency, wind dryness and blood stasis, reduce the levels of serum hs-CRP, IL-6, P, PTH and β2-MG, increase serum Ca levels, improve micro-inflammatory state and calcium-phosphorus metabolism problems to a certain extent, and increase the HGB level of patients, which can improve anemia to a certain extent.

[0004] Jasminum pentaneurum Hand.-Mazz. is a fresh branch and leaf of Jasminum pentaneurum Hand.-Mazz. of the Oleaceae family. It has a light taste and cool nature. It has the effect of removing blood stasis and detoxifying. It is mainly used to treat injuries from falls, sore throat, mouth sores, snake bites and other diseases. In the Flora of China and the Chinese Materia Medica, there are a few records of the medicinal use of Jasminum pentaneurum. Jasminum pentaneurum is mainly used to remove blood stasis and detoxify. It is mainly used to treat injuries from falls, sore throat, mouth sores, boils, and snake bites.

[0005] At present, there is no report on the research of using Jasminum crassifolium for treating skin itching. Therefore, providing the use of Jasminum crassifolium and its extracts in the preparation of drugs for treating skin itching is a technical problem that needs to be solved urgently by those skilled in the art. Summary of the invention

[0006] In view of this, the present invention provides the use of Jasmine thunbergii and its extract in preparing a drug for treating skin itching. The present invention firstly finds that Jasmine thunbergii and its extract have the potential to be a drug for treating skin itching, and can be used to prepare a drug for treating skin itching, thus opening up a new way for treating skin itching. The preparation method of the drug preparation is simple, the cost is low, and the economic benefit of the drug is good.

[0007] To achieve the above object, the present invention adopts the following technical solution:

[0008] The invention provides application of Jasmine crassifolia and an extract thereof in preparing a medicine for treating skin itching.

[0009] Furthermore, the Jasmine thunbergii extract is a water extract of fresh branches and leaves of Jasmine thunbergii, or an organic solvent extract of fresh branches and leaves of Jasmine thunbergii.

[0010] Further, the preparation method of the water extract of fresh branches and leaves of Jasmine thunbergii is:

[0011] (1) Take fresh Jasmine jasminoides branches and leaves, add 6 to 10 times the weight of water, heat and boil to extract for 30 to 60 minutes, filter, and collect the filtrate;

[0012] (2) Add 6 to 10 times the weight of water to the residue, heat and boil again for 30 to 60 minutes, filter, and collect the filtrate;

[0013] (3) Repeat step (2) 1 to 3 times, combine the filtrates obtained from multiple extractions and concentrate them to obtain an aqueous extract of fresh branches and leaves of Jasmine thunbergii.

[0014] Furthermore, the organic solvent extract of fresh branches and leaves of Jasmine thunbergii is an ethanol extract of fresh branches and leaves of Jasmine thunbergii.

[0015] Furthermore, the preparation method of the ethanol extract of fresh branches and leaves of Jasmine thunbergii is:

[0016] 1) Take fresh Jasmine thunbergii branches and leaves, add 6 to 10 times the weight of ethanol, heat and reflux for 30 to 60 minutes, filter, and collect the filtrate;

[0017] 2) Add 6 to 10 times the mass of ethanol to the residue, heat and reflux for 30 to 60 minutes, filter, and collect the filtrate;

[0018] 3) Repeat step 2) 1 to 3 times, combine the filtrates obtained by multiple extractions, and concentrate under reduced pressure to recover ethanol, thereby obtaining the ethanol extract of fresh branches and leaves of Jasmine thunbergii.

[0019] Furthermore, the volume concentration of the ethanol is 30-50%.

[0020] Furthermore, the drug for treating skin itching is a drug preparation for treating skin itching prepared by adding conventional excipients.

[0021] Furthermore, the pharmaceutical preparation is a lotion, an ointment, a paste, a patch, a spray or a liniment.

[0022] Furthermore, the conventional auxiliary material is a mixture of one or more of sodium lauryl sulfate, ethylparaben and glycerol.

[0023] The beneficial effects of the present invention are:

[0024] The present invention discovers for the first time that Jasmine thunbergii and its extract have the potential to be a medicine for treating skin itching, and can be used to prepare a medicine for treating skin itching, thus opening up a new approach for the treatment of skin itching.

[0025] The Jasmine thunbergii resource of the invention is rich, the extraction and preparation method is simple and easy, the Jasmine thunbergii source is convenient and easy to obtain, and can be used for mass preparation of Jasmine thunbergii medicinal materials and extracts thereof. The scheme of the invention has low cost and little pollution, is conducive to large-scale production under the conditions of energy conservation and emission reduction, and has good industrialization prospects.

[0026] The present invention provides the therapeutic effect of the Jasmine thunbergii extract on itchy mice, and proves that the Jasmine thunbergii extract can reduce the number of scratching times of mice and improve the itchy behavior of mouse skin induced by compound 48 / 80 (65mmol / L), which fully proves that the Jasmine thunbergii extract of the present invention has a significant antipruritic effect on itchy mice. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 To study the effects of the water and alcohol extracts of Jasmine crassifolia on the scratching frequency of itch-prone mice;

[0028] Figure 2 To investigate the effects of Jasmine thunbergii water extract and ethanol extract on the distribution of mast cells in the skin of pruritic mice.

[0029] Figure 3 To investigate the effects of Jasmine thunbergii aqueous and alcohol extracts on the number of mast cells in the skin of pruritic mice. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] In the embodiment of the present invention, the concentrated Jasmine thunbergii extract is 1.6 g medicinal material / mL, which means that the volume ratio of the mass of the fresh branches and leaves of Jasmine thunbergii to the concentrated extract is 1.6 g medicinal material / mL.

[0032] Example 1 Preparation of Jasmine Thick-leaved Extract

[0033] Take 1 kg of fresh branches and leaves of Jasmine thunbergii, add 8 L of distilled water, then heat and boil to extract for 45 minutes, filter, and collect the filtrate; add 8 L of distilled water to the filter residue, heat and boil to extract for 45 minutes, filter, and collect the filtrate; add 8 L of distilled water to the secondary filter residue, heat and boil to extract for 45 minutes, filter, and collect the filtrate; combine the three extracts and concentrate to 1.6 g of medicinal material / mL (85°C) to obtain Jasmine thunbergii extract.

[0034] Example 2 Preparation of Jasmine Thick-leaved Extract

[0035] Take 1 kg of fresh branches and leaves of Jasmine thunbergii, add 10 L of distilled water, then heat and boil to extract for 30 minutes, filter, and collect the filtrate; add 10 L of distilled water to the filter residue, heat and boil to extract for 30 minutes, filter, and collect the filtrate; add 10 L of distilled water to the secondary filter residue, heat and boil to extract for 30 minutes, filter, and collect the filtrate; combine the three extracts and concentrate to 1.6 g medicinal material / mL (85°C) to obtain Jasmine thunbergii extract.

[0036] Example 3 Preparation of Jasmine Thick-leaved Extract

[0037] Take 1 kg of fresh branches and leaves of Jasmine thunbergii, add 6 L of distilled water, then heat and boil to extract for 60 minutes, filter, and collect the filtrate; add 6 L of distilled water to the filter residue, heat and boil to extract for 60 minutes, filter, and collect the filtrate; add 6 L of distilled water to the secondary filter residue, heat and boil to extract for 60 minutes, filter, and collect the filtrate; combine the three extracts and concentrate to 1.6 g of medicinal material / mL (85°C) to obtain Jasmine thunbergii extract.

[0038] Example 4 Preparation of Jasmine Thick-leaved Extract

[0039] Take 1 kg of fresh branches and leaves of Jasmine thunbergii, add 7 L of 50% ethanol by volume, then heat to 80°C and reflux for extraction for 40 minutes, filter and collect the filtrate; add 7 L of 50% ethanol by volume to the filter residue, heat to 80°C and reflux for extraction for 40 minutes, filter and collect the filtrate; add 7 L of 50% ethanol by volume to the secondary filter residue, heat to 80°C and extract for 40 minutes, filter and collect the filtrate; combine the three extracts and concentrate under reduced pressure to recover ethanol, and concentrate to 1.6 g medicinal material / mL (85°C) to obtain Jasmine thunbergii extract.

[0040] Example 5 Preparation of Jasmine Thick-leaved Extract

[0041] Take 1 kg of fresh branches and leaves of Jasmine thunbergii, add 10 L of 30% ethanol, then heat to 85°C and reflux for extraction for 60 min, filter and collect the filtrate; add 10 L of 30% ethanol to the filter residue, continue to heat to 85°C and reflux for extraction for 60 min, filter and collect the filtrate; add 10 L of 30% ethanol to the secondary filter residue, continue to heat to 85°C and reflux for extraction for 60 min, filter and collect the filtrate; combine the three extracts and concentrate under reduced pressure to recover ethanol, and concentrate to 1.6 g medicinal material / mL (85°C) to obtain Jasmine thunbergii extract.

[0042] The extracts provided in Examples 1 to 5 above can be further prepared into external preparations according to a pharmaceutically acceptable method. The external preparations can be ointments, pastes, patches, lotions, sprays, liniments, etc. Here, the external lotion is used as an example for description.

[0043] Example 6 Preparation of Jasmine Thick-leaved Extract Lotion

[0044] Prescription: The prepared Jasmine thunbergii water extract (the amount equivalent to 3 kg of Jasmine thunbergii medicinal material) is prepared according to the following method:

[0045] Weigh 1600g of fresh branches and leaves of Jasmine thunbergii, crush them, add water equivalent to 6 times of its total weight for the first time, boil for 1.0h, and collect the filtrate; add water equivalent to 6 times of its total weight to the residue, boil for 1.0h, and collect the filtrate; add water equivalent to 6 times of its total weight to the residue, boil for 1.0h, and collect the filtrate. Combine the filtrates obtained from the three decoctions and concentrate to 1.6g medicinal material / mL (85℃);

[0046] Add 6 g of sodium lauryl sulfate, 1 g of ethylparaben and 10 g of glycerol to the obtained concentrated solution, mix and stir thoroughly until dissolved, add purified water to a total volume of 1000 ml, and fill in portions to obtain the product.

[0047] The above-mentioned sodium lauryl sulfate, ethylparaben and glycerin can all be purchased on the market, and the added amounts meet the requirements of relevant standards.

[0048] Test Example 1: Drug Activity Test

[0049] In order to verify the antipruritic effect of the Jasmine thunbergii extract, the following test was conducted:

[0050] To verify the antipruritic effect of Jasmine thunbergii extract on itchy mice.

[0051] Test sample: Jasmine thunbergii water extract prepared by the method described in Example 3. Jasmine thunbergii 50% alcohol extract prepared by the method described in Example 4. Jasmine thunbergii 30% alcohol extract prepared by the method described in Example 5. The high-dose group of extracts corresponded to the extracts of the concentrations in the examples, and the low-dose solutions were diluted 1 times with pure water at the concentration of the high-dose solution.

[0052] Treatment method:

[0053] ICR mice were taken and adaptively fed for 3 days, and then randomly divided into the following groups, with 6 mice in each group: blank control group, dexamethasone acetate group (positive control group), high dose of Jasmine thick-leaved water extract (HYSX-high group, 80 g medicinal material / kg), low dose of Jasmine thick-leaved water extract (HYSX-low group, 40 g medicinal material / kg), high dose of 30% ethanol extract of Jasmine thick-leaved (HYSX-30-high group, 80 g medicinal material / kg), low dose of 30% ethanol extract of Jasmine thick-leaved (HYSX-30-low group, 40 g medicinal material / kg), high dose of 50% ethanol extract of Jasmine thick-leaved (HYSX-50-high group, 80 g medicinal material / kg), and low dose of 50% ethanol extract of Jasmine thick-leaved (HYSX-50-low group, 40 g medicinal material / kg).

[0054] One day before the experiment, hair was removed from the back of the neck of the mouse with a clipper to a size of 2 cm × 2 cm, and mice with undamaged skin were selected for the experiment. Each dose group applied 1 ml of the corresponding lotion to the depilated area, and the positive control group applied 0.2 g of dexamethasone acetate to each mouse. The drug was administered for 5 consecutive days. One hour after the last administration, 50 μL of compound 48 / 80 (65 mmol / L) was subcutaneously injected into the back of the neck of the mouse, and the scratching reaction of the mouse was immediately observed within 30 minutes. The calculation was based on one attack, that is, from the time the hind limbs were raised to touch the injection site of the itch-inducing agent to the time the hind limbs were placed back on the ground or in the mouth, the whole process was regarded as one scratch, and the number of scratches was counted. The number of scratches when the mouse scratched was recorded. After the observation, the skin was taken, fixed in 4% formaldehyde, stained with toluidine blue, and the number of mast cells was counted.

[0055] Results: Figure 1-3 As shown, dexamethasone acetate can significantly reduce the number of scratching times and the number of subcutaneous mast cells (purple) in mice. The high and low dose groups of Jasmine thunbergii water extract and the high and low dose groups of 50% alcohol extract can significantly reduce the number of scratching times in mice and improve the itch behavior of mouse skin induced by compound 48 / 80 (65mmol / L) (p<0.05, p<0.01) (as shown in Figure 2). Figure 1 The low-dose group of Jasmine thunbergii water extract and the low-dose group of 50% alcohol extract could significantly reduce the number of subcutaneous mast cells in mice (p<0.05, p<0.01), indicating that Jasmine thunbergii water extract and alcohol extract have the effect of inhibiting the release of itch-related particles from mast cells.

[0056] Note: *P<0.05, **P<0.01 vs. blank group.

[0057] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. Application of Jasmine thunbergii and its extract in the preparation of medicines for treating skin itching.

2. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 1, characterized in that: The jasmine thunbergii extract is a water extract of fresh branches and leaves of jasmine thunbergii, or an organic solvent extract of fresh branches and leaves of jasmine thunbergii.

3. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 2, characterized in that: The preparation method of the water extract of fresh branches and leaves of Jasmine thunbergii is as follows: (1) Take fresh Jasmine jasminoides branches and leaves, add 6 to 10 times the weight of water, heat and boil to extract for 30 to 60 minutes, filter, and collect the filtrate; (2) Add 6 to 10 times the weight of water to the residue, heat and boil again for 30 to 60 minutes, filter, and collect the filtrate; (3) Repeat step (2) 1 to 3 times, combine the filtrates obtained from multiple extractions and concentrate them to obtain an aqueous extract of fresh branches and leaves of Jasmine thunbergii.

4. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 2, characterized in that: The organic solvent extract of fresh branches and leaves of Jasmine thunbergii is an ethanol extract of fresh branches and leaves of Jasmine thunbergii.

5. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 4, characterized in that: The preparation method of the ethanol extract of fresh branches and leaves of Jasmine thunbergii is as follows: 1) Take fresh Jasmine thunbergii branches and leaves, add 6 to 10 times the weight of ethanol, heat and reflux for 30 to 60 minutes, filter, and collect the filtrate; 2) Add 6 to 10 times the mass of ethanol to the residue, heat and reflux for 30 to 60 minutes, filter, and collect the filtrate; 3) Repeat step 2) 1 to 3 times, combine the filtrates obtained by multiple extractions, and concentrate under reduced pressure to recover ethanol, thereby obtaining the ethanol extract of fresh branches and leaves of Jasmine thunbergii.

6. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 5, characterized in that: The volume concentration of the ethanol is 30-50%.

7. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 1, characterized in that: The medicine for treating skin itching is a medicine preparation for treating skin itching prepared by adding conventional excipients.

8. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 7, characterized in that: The pharmaceutical preparation is a lotion, an ointment, a paste, a patch, a spray or a liniment.

9. The use of Jasmine thunbergii and its extract in the preparation of a medicine for treating skin itching according to claim 7, characterized in that: The conventional auxiliary material is a mixture of any one or more of sodium lauryl sulfate, ethylparaben and glycerol.

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