Composition for improving levels of endogenous gamma-aminobutyric acid and melatonin and application thereof

Abstract

The invention relates to a composition for improving levels of endogenous gamma-aminobutyric acid and melatonin and application of the composition. The composition comprises a pseudo-ginseng stem and leaf compound, gamma-aminobutyric acid, L-theanine and a liquorice extract, the pseudo-ginseng stem and leaf compound is a product obtained by alcohol extraction of pseudo-ginseng stems and leaves, gastrodia elata, poria cocos and spina date seeds as raw materials. The pseudo-ginseng stem and leaf compound is developed firstly, the pseudo-ginseng stem and leaf compound is obtained by taking four specific medicinal and edible materials including pseudo-ginseng stems and leaves, gastrodia elata, poria cocos and spina date seeds as raw materials through alcohol extraction, and the four raw materials are matched with one another and have a potential mutual promotion effect in the aspect of improving endogenous gamma-aminobutyric acid and melatonin. The pseudo-ginseng stem and leaf compound is creatively combined with gamma-aminobutyric acid, L-theanine and a licorice extract for use, and the four components cooperate with each other, so that the effect of improving the levels of endogenous gamma-aminobutyric acid and endogenous melatonin is further improved.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extracts and relates to a composition for improving the levels of endogenous gamma-aminobutyric acid and melatonin and an application thereof. Background Art

[0002] One third of our life is spent sleeping, and the relationship between sleep and health has always been valued by people. Good sleep can eliminate fatigue, restore mental and physical strength, maintain a good state of wakefulness, improve work and study efficiency, and prolong life. Insomnia is mainly manifested as difficulty falling asleep, waking up easily during shallow sleep, difficulty falling asleep after waking up, and dreaming, often accompanied by daytime malaise, limb weakness, slow reaction, low work efficiency, headache, memory loss and other symptoms.

[0003] Current sleep aid products may have the following defects: First, their mechanism of action is relatively simple, mainly helping sleep by inhibiting neuronal excitation. For sleep problems caused by a variety of complex factors such as psychology and environment, the effect may be limited; second, individual differences have a greater impact, and different people's nervous systems have different sensitivities to γ-aminobutyric acid. Some people may respond less to it and fail to achieve the ideal sleep-aiding effect; third, long-term use may lead to tolerance. Over time, the body may gradually adapt to its effects, and an increase in dosage is required to maintain the same effect. In addition, long-term exogenous intake of melatonin may interfere with the body's normal secretion mechanism of melatonin, causing the body to become dependent on it, which in turn affects its ability to regulate sleep. In addition, the effect of exogenous melatonin intake may also be strictly limited by age, usage time and dosage. Improper use may cause side effects such as headaches, drowsiness, and nightmares.

[0004] GABA, also known as aminobutyric acid, is an important inhibitory neurotransmitter naturally present in the central nervous system of mammals. Its main function is to inhibit the overexcitation of neurons, thereby maintaining the balance and stability of the nervous system. As the endogenous GABA content decreases, it may cause insomnia, anxiety and other problems.

[0005] Melatonin is an amine hormone secreted by the pineal gland in the brain. Its secretion has a clear circadian rhythm, with secretion suppressed during the day and active at night. The synthesis of melatonin is closely related to light. When the ambient light dims, the retina senses this change and transmits signals to the pineal gland in the brain, prompting the pineal gland to begin secreting large amounts of melatonin. Melatonin plays a key role in regulating sleep, but long-term exogenous excessive use of melatonin may cause side effects such as headaches, dizziness, and nausea. Endogenously increasing the content of melatonin is a way to regulate melatonin secretion in the human body, which does not have these side effects and is a safe way to regulate sleep.

[0006] Therefore, it would be very meaningful to develop a product that can simultaneously increase the levels of endogenous GABA and endogenous melatonin. Summary of the invention

[0007] In view of the deficiencies of the prior art, the object of the present invention is to provide a composition for increasing the levels of endogenous γ-aminobutyric acid and melatonin and its application.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a composition for improving the levels of endogenous γ-aminobutyric acid and melatonin, the composition comprising a Panax notoginseng stem and leaf complex, γ-aminobutyric acid, L-theanine and licorice extract; the Panax notoginseng stem and leaf complex is a product obtained by alcohol extraction using Panax notoginseng stem and leaf, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds as raw materials.

[0010] The present invention firstly develops a Panax notoginseng stem and leaf complex, which is obtained by alcohol extraction using four specific medicinal and edible materials, namely, Panax notoginseng stem and leaf, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds, as raw materials. The Panax notoginseng stem and leaf are rich in ginsenosides and notoginseng saponin Fc, among which the higher contents of ginsenosides Rb2, Ra1, Ra2, notoginseng saponin Fc, amino acids and trace elements can improve blood circulation and provide better nutrients and biological activity for the nervous system; Gastrodia elata is rich in gastrodin, which can calm the liver and relieve wind, and relieve nervous tension; Poria cocos has the effects of strengthening the spleen and calming the mind, and regulating the metabolic balance in the body; Ziziphus jujuba contains components such as Ziziphus jujuba seeds saponin, which has the effects of nourishing the heart and calming the nerves. The four raw materials cooperate with each other and have a potential mutual promotion effect in improving endogenous γ-aminobutyric acid and endogenous melatonin. The present invention also creatively combines the Panax notoginseng stem and leaf complex with γ-aminobutyric acid, L-theanine and licorice extract. The four components work synergistically with each other to further improve the effect of increasing the levels of endogenous γ-aminobutyric acid and endogenous melatonin.

[0011] Preferably, the Panax notoginseng stem and leaf composite is prepared by a preparation method comprising the following steps:

[0012] The raw materials of preparation, namely, Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds, are mixed with ethanol aqueous solution for extraction, the extraction product is filtered, and the filtrate is dried to obtain the product.

[0013] Preferably, the mass ratio of Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds is (4-8):(1-3):(1-3):1.

[0014] The specific point values ​​in (4-8) can be selected as 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, etc.; the specific point values ​​in (1-3) can be selected as 1, 1.3, 1.5, 1.7, 2, 2.2, 2.5, 2.6, 2.8, 3, etc.; other specific point values ​​within this numerical range can be selected and will not be listed here one by one.

[0015] When the stems and leaves of Panax notoginseng, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds are combined in the above-mentioned specific mass ratio, the product has a better effect in increasing the levels of endogenous γ-aminobutyric acid and endogenous melatonin.

[0016] Preferably, the volume fraction of the ethanol aqueous solution is 15-25%, for example, 15%, 18%, 20%, 22%, 25%, etc.

[0017] Compared with other ethanol concentrations, when the ethanol concentration of the ethanol aqueous solution is 10-30%, the product has a better effect in increasing the levels of endogenous γ-aminobutyric acid and endogenous melatonin.

[0018] Preferably, the solid-liquid ratio of the preparation raw material to the ethanol aqueous solution is 1:(15-35) g / mL, for example 1:15 g / mL, 1:18 g / mL, 1:20 g / mL, 1:22 g / mL, 1:25 g / mL, 1:30 g / mL, 1:35 g / mL, etc.

[0019] Preferably, the temperature of the leaching treatment is 15-45°C, for example, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 45°C, etc.

[0020] Preferably, the extraction is performed 1-3 times, for example 1 time, 2 times or 3 times; the time for each extraction is 20-80 hours, for example 20 hours, 30 hours, 35 hours, 40 hours, 45 hours, 50 hours, 55 hours, 60 hours, 65 hours, 70 hours, 75 hours, 80 hours, etc.

[0021] Other specific point values ​​within the above-mentioned numerical ranges can be selected and will not be described in detail here.

[0022] Preferably, the composition comprises, by mass, 10-30 parts of Panax notoginseng stem and leaf complex, 30-50 parts of γ-aminobutyric acid, 30-50 parts of L-theanine and 0.1-1 part of liquorice extract.

[0023] When the Panax notoginseng stem and leaf complex, gamma-aminobutyric acid, L-theanine and licorice extract are combined in the above specific mass ratio, the composition has a better effect in increasing the levels of endogenous gamma-aminobutyric acid and endogenous melatonin.

[0024] The mass fraction of the Panax notoginseng stem and leaf complex can be selected as 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, etc.; the mass fraction of the γ-aminobutyric acid can be selected as 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 ​​parts, 50 parts, etc.; the mass fraction of the L-theanine can be selected as 30 parts, 32 parts, 35 parts, 38 parts, 40 parts, 42 parts, 45 parts, 48 ​​parts, 50 parts, etc.; the mass fraction of the licorice extract can be selected as 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 1 parts, etc.

[0025] Other specific point values ​​within the above-mentioned numerical ranges can be selected and will not be described in detail here.

[0026] In a second aspect, the present invention provides use of the composition according to the first aspect in preparing a product having rich triterpenoid saponins and amino acid bioactive ingredients.

[0027] In a third aspect, the present invention provides use of the composition according to the first aspect in preparing a product for improving sleep quality.

[0028] Gamma-aminobutyric acid is a neuro-inhibitory amino acid that can inhibit excessive nerve excitement and improve sleep quality; melatonin mainly participates in regulating sleep function by acting on the melatonin receptors EMT1 and MT2 in the hypothalamus and optic chiasma. The composition of the present invention has excellent efficacy in increasing the levels of endogenous gamma-aminobutyric acid and melatonin, and its application in the preparation of products that improve sleep quality has broad prospects.

[0029] In a fourth aspect, the present invention provides use of the composition according to the first aspect in preparing a product having antioxidant activity.

[0030] Furthermore, the present invention has also found that the aforementioned composition not only has excellent efficacy in increasing the levels of γ-aminobutyric acid and endogenous melatonin, but also has excellent antioxidant efficacy, and therefore, it can be used to prepare products with antioxidant activity.

[0031] In a fifth aspect, the present invention provides use of the composition according to the first aspect in preparing a product having α-glucosidase inhibitory activity.

[0032] Furthermore, the present invention has also found that the aforementioned composition not only has excellent efficacy in increasing the levels of γ-aminobutyric acid and endogenous melatonin, but also has excellent efficacy in inhibiting the activity of α-glucosidase. α-glucosidase can promote the conversion of carbohydrates ingested by the human body into glucose to be absorbed by the small intestine, thereby increasing blood sugar levels. Inhibiting the activity of α-glucosidase can promote blood sugar stability and prevent the occurrence of diabetes. Therefore, it can also be used to prepare products that promote blood sugar stability and prevent and treat diabetes.

[0033] In a sixth aspect, the present invention provides use of the composition according to the first aspect in preparing a product for increasing endogenous melatonin levels.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The present invention first develops a Panax notoginseng stem and leaf complex, which is obtained by alcohol extraction using four specific medicinal and edible materials, namely Panax notoginseng stem and leaf, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds, as raw materials. Among them, Panax notoginseng stem and leaf can improve blood circulation and provide better nutrients for the nervous system; Gastrodia elata can calm the liver and relieve wind, relieve nervous tension; Poria cocos can invigorate the spleen and calm the mind, and regulate the metabolic balance in the body; Ziziphus jujuba seeds can nourish the heart and calm the mind. These four raw materials cooperate with each other and have a potential mutual promotion effect in improving endogenous γ-aminobutyric acid and endogenous melatonin. The present invention also creatively combines the Panax notoginseng stem and leaf complex with γ-aminobutyric acid, L-theanine and licorice extract, and the four components work together to further improve the effect of improving the level of endogenous γ-aminobutyric acid and endogenous endogenous melatonin. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is the test result of monomer saponin in the composite of Panax notoginseng stem and leaf;

[0037] Figure 2 This is the test result of amino acids in the Panax notoginseng stem and leaf complex; DETAILED DESCRIPTION

[0038] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0039] The sources of some of the raw materials involved in the following content are as follows:

[0040] Panax notoginseng stems and leaves: provided by the National and Local Joint Engineering Research Center for the Innovation and Utilization of Traditional Chinese Medicine Germplasm in Southwest China, and identified as the dried stems and leaves of Panax notoginseng of the Araliaceae family. Licorice extract: provided by the National and Local Joint Engineering Research Center for the Innovation and Utilization of Traditional Chinese Medicine Germplasm in Southwest China. γ-Aminobutyric acid (GABA): purchased from Sichuan Shengshi Biopharmaceutical Company. L-Theanine: Hebei Huayang Biotechnology Co., Ltd. Gastrodia elata, Poria cocos, and Ziziphus jujuba seeds are commercially available. All of the above materials are food grade.

[0041] Preparation Example 1

[0042] This preparation example provides a Panax notoginseng stem and leaf composite, and the preparation method thereof is as follows:

[0043] Mix Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds in a mass ratio of 6:2:2:1 with 20% ethanol aqueous solution at a solid-liquid ratio of 1:25 g / mL, and perform extraction at 35°C for 60 h, for a total of 2 extractions. Combine the extracted products and filter them. Heat the filtrate in a water bath under boiling water conditions to evaporate until there is no moisture on the surface, and then put it into a drying oven to dry until constant weight.

[0044] Preparation Example 2

[0045] This preparation example provides a Panax notoginseng stem and leaf composite, and the preparation method thereof is as follows:

[0046] Mix Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds in a mass ratio of 8:1:1:1 with 30% ethanol aqueous solution at a solid-liquid ratio of 1:15 g / mL, and perform extraction at 30°C for 65 h, for a total of 2 extractions. Combine the extracted products and filter them. Heat the filtrate in a water bath under boiling water conditions to evaporate until there is no moisture on the surface, and then put it into a drying oven to dry until constant weight.

[0047] Preparation Example 3

[0048] This preparation example provides a Panax notoginseng stem and leaf composite, and the preparation method thereof is as follows:

[0049] Mix Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds in a mass ratio of 4:3:3:1 with 10% ethanol aqueous solution at a solid-liquid ratio of 1:35 g / mL, and perform extraction at 40°C for 55 h, for a total of 2 extractions. Combine the extracted products and filter them. Heat the filtrate in a water bath under boiling water conditions to evaporate until there is no moisture on the surface, and then put it into a drying oven to dry until constant weight.

[0050] Preparation Example 4

[0051] This preparation example provides a Panax notoginseng stem and leaf complex, and the preparation method thereof is different from that of Preparation Example 1 only in that an equal volume of 20% ethanol aqueous solution is replaced by 30% ethanol aqueous solution, and other conditions remain unchanged.

[0052] Preparation Example 5

[0053] This preparation example provides a Panax notoginseng stem and leaf complex, and the preparation method thereof is different from that of Preparation Example 1 only in that an equal volume of 20% ethanol aqueous solution is replaced by 10% ethanol aqueous solution, and other conditions remain unchanged.

[0054] Comparative Preparation Example 1

[0055] This comparative preparation example provides a composite, and its preparation method is different from that of Preparation Example 1 only in that: the stems and leaves of Panax notoginseng are missing, and their reduced mass is proportionally distributed to the mass of Gastrodia elata, Poria cocos and Ziziphus jujuba seeds, and other conditions remain unchanged.

[0056] Comparative Preparation Example 2

[0057] This comparative preparation example provides a composite, and its preparation method is different from that of Preparation Example 1 only in that Gastrodia elata is missing, and its reduced mass is proportionally distributed to the masses of Panax notoginseng stems and leaves, Poria cocos and Ziziphus jujuba seeds, and other conditions remain unchanged.

[0058] Comparative Preparation Example 3

[0059] This comparative preparation example provides a composite, and its preparation method is different from that of Preparation Example 1 only in that: Poria cocos is missing, and its reduced mass is proportionally distributed to the masses of Panax notoginseng stems and leaves, Gastrodia elata and Ziziphus jujuba seeds, and other conditions remain unchanged.

[0060] Comparative Preparation Example 4

[0061] This comparative preparation example provides a composite, and its preparation method is different from that of Preparation Example 1 only in that: the Chinese date seed is missing, and its reduced mass is proportionally distributed to the mass of Panax notoginseng stems and leaves, Gastrodia elata and Poria cocos, and other conditions remain unchanged.

[0062] Example 1

[0063] This embodiment provides a composition, the components of which include, by mass, 20 parts of the Panax notoginseng stem and leaf complex of Preparation Example 1, 40 parts of γ-aminobutyric acid, 40 parts of L-theanine, and 0.2 parts of licorice extract. The components are mixed and crushed and then passed through a 120-mesh sieve to obtain a composition.

[0064] Example 2

[0065] This embodiment provides a composition, the components of which include, by mass, 30 parts of the Panax notoginseng stem and leaf complex of Preparation Example 2, 30 parts of γ-aminobutyric acid, 30 parts of L-theanine, and 0.5 parts of licorice extract. The components are mixed and crushed and then passed through a 120-mesh sieve to obtain a composition.

[0066] Example 3

[0067] This embodiment provides a composition, the components of which include, by mass, 10 parts of the Panax notoginseng stem and leaf complex of Preparation Example 3, 50 parts of γ-aminobutyric acid, 50 parts of L-theanine, and 0.1 parts of licorice extract. The components are mixed and crushed and then passed through a 120-mesh sieve to obtain a composition.

[0068] Embodiment 4-5

[0069] This example provides two compositions, the components of which are different from those of Example 1 only in that the Panax notoginseng stem and leaf complex of Preparation Example 1 is replaced by the Panax notoginseng stem and leaf complex of Preparation Examples 4-5 in equal amounts, respectively. The rest remain unchanged.

[0070] Comparative Examples 1-4

[0071] This comparative example provides four compositions, the components of which are different from those of Example 1 only in that the Panax notoginseng stem and leaf complex of Preparation Example 1 is replaced by the Panax notoginseng stem and leaf complex of Comparative Preparation Examples 1 to 4 in equal amounts, respectively. The rest remain unchanged.

[0072] Comparative Example 5

[0073] This comparative example provides a composition, the components of which differ from those of Example 1 only in that the Panax notoginseng stem and leaf complex is missing, and the missing mass is proportionally distributed to the masses of γ-aminobutyric acid, L-theanine and licorice extract, while the others remain unchanged.

[0074] Comparative Example 6

[0075] This comparative example provides a composition, the components of which differ from those of Example 1 only in the lack of γ-aminobutyric acid, and the missing mass is proportionally distributed to the masses of the notoginseng stem and leaf complex, L-theanine and licorice extract, while the others remain unchanged.

[0076] Comparative Example 7

[0077] This comparative example provides a composition, the components of which differ from those of Example 1 only in the lack of L-theanine, and the lacking mass is proportionally distributed to the masses of the notoginseng stem and leaf complex, γ-aminobutyric acid and licorice extract, while the others remain unchanged.

[0078] Comparative Example 8

[0079] This comparative example provides a composition, the components of which differ from those of Example 1 only in the lack of licorice extract, and the lacking mass is proportionally distributed to the masses of Panax notoginseng stem and leaf complex, γ-aminobutyric acid and L-theanine, while the others remain unchanged.

[0080] Test Example 1

[0081] Results of the main bioactive components in the Panax notoginseng stem and leaf complex:

[0082] (1) Types and contents of monomeric saponins

[0083] The content of triterpenoid saponins in the Panax notoginseng stem and leaf composite prepared in Preparation Example 1 was determined by high performance liquid chromatography. Figure 1 As shown in the figure, the triterpenoid saponins in the composition mainly include ginsenoside S-Rh1, S-Rh2, S-Rg2, Ra2, Ra3, Rb2, Rb3, Rg5, Ra1, notoginsenoside Fc, gastrodin, and jujuboside A, among which the content of ginsenoside Rb2 is 41.16 mg / g, the content of Ra2 is 35.11 mg / g, the content of Ra1 is 27.69 mg / g, and the content of notoginsenoside Fc is 38.74 mg / g.

[0084] (2) Types and contents of amino acids

[0085] A total of 18 amino acids were detected from the Panax notoginseng stem and leaf complex prepared in Preparation Example 1, such as Figure 2 As shown, they are: Sap, Glu, Ser, His, Gly, Thr, Ala, Theanine, Arg, Tyr, Cys, GABA, Val, Met, Phe, Lle, Leu, Pro. Among them, the amino acids with higher content are mainly: Theanine (6.720mg / g), GABA (5.049mg / g), Tyr (5.048mg / g).

[0086] Test Example 2

[0087] Effects of the composition on endogenous GABA and melatonin levels:

[0088] (1) Safety evaluation:

[0089] Healthy zebrafish fry at 5 dpf after fertilization were selected and placed in a six-well plate, with 20 fish per well and 3 parallel groups. Samples of Example 1 at concentrations of 0 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL, 250 μg / mL, 500 μg / mL, 1000 μg / mL, and 2000 μg / mL were added respectively. The samples were incubated in a 28°C incubator for 1 day, and the mortality rate of each concentration was counted. The results are shown in Table 1.

[0090] Table 1

[0091]

[0092] It can be seen from the results in Table 1 that the composition of the present invention is safe and non-toxic to zebrafish embryos.

[0093] (2) Experimental groups: divided into a blank control group, a model group (600 μg / mL pentylenetetrazol), and a sample group (including samples of Examples 1-5 and Comparative Examples 1-8, with a test concentration of 1000 μg / mL), with 3 parallel groups in each group.

[0094] (3) Construction and intervention of zebrafish insomnia model:

[0095] The experimental design was to use 5dpf wild-type AB zebrafish to construct an insomnia model, which was randomly divided into 15 groups, with 30 fish in each group. Except for the blank control group, the others were stimulated with 600μg / mL PTZ for 1 hour to establish the zebrafish insomnia model. After PTZ stimulation for 1 hour, PTZ was removed and different concentrations of test samples were added for incubation at 28°C for 24 hours.

[0096] (4) Index detection:

[0097] After the treatment, the zebrafish in each group were homogenized, and the average levels of γ-aminobutyric acid and melatonin in different groups were detected using γ-aminobutyric acid and melatonin assay kits. The results are shown in Table 2.

[0098] Table 2

[0099]

[0100]

[0101] It can be seen from the data results in Table 2 that, compared with Comparative Examples 1-8, the composition of the present invention selects specific raw materials to prepare a Panax notoginseng stem and leaf complex, and compounding it with γ-aminobutyric acid, L-theanine and licorice extract. The components work synergistically with each other to obtain an excellent effect of increasing the levels of γ-aminobutyric acid and melatonin, especially the effect of increasing the level of endogenous melatonin.

[0102] Test Example 3

[0103] Evaluation of DPPH antioxidant activity of the composition:

[0104] The compositions prepared in Examples 1-5 and Comparative Examples 1-8 were diluted with deionized water to a concentration of 6 mg / mL as test sample solutions.

[0105] Test method: Determine the scavenging ability of the composition on DPPH free radicals according to the instructions of the kit, that is, use 300 μL of 50% ethanol as a blank control, draw 50 μL of each of the above test samples into 250 μL, 120 μmol / L DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) solution, add 50% ethanol to 300 μL, react at 25°C for 30 minutes, measure the absorbance value (A) at 517 nm, replace the sample with the same dose of 50% ethanol and add it to the same volume of DPPH solution to measure the maximum absorbance of DPPH (A0), take another equal amount of the test solution and add it to 50% ethanol to eliminate the influence of the sample itself on the absorbance value (A 样). Carry out 3 parallel experiments, calculate DPPH clearance rate according to the following formula, and take the average value:

[0106] DPPH clearance rate (%) = (A0-(AA sample)) / A0*100%

[0107] The results are shown in Table 3.

[0108] Table 3

[0109] Group DPPH clearance Example 1 88.40％ Example 2 86.06％ Example 3 87.35％ Example 4 83.09％ Example 5 84.67％ Comparative Example 1 71.98％ Comparative Example 2 79.77％ Comparative Example 3 75.65％ Comparative Example 4 76.90％ Comparative Example 5 62.93％ Comparative Example 6 67.67％ Comparative Example 7 70.89％ Comparative Example 8 71.33％

[0110] It can be seen from the data results in Table 3 that, compared with Comparative Examples 1-8, the composition of the present invention selects specific raw materials to prepare a Panax notoginseng stem and leaf complex, and compoundes it with γ-aminobutyric acid, L-theanine and licorice extract. The components work synergistically to achieve excellent antioxidant effects.

[0111] Test Example 4

[0112] Evaluation of α-glucosidase inhibitory activity of the composition:

[0113] The compositions prepared in Examples 1-5 and Comparative Examples 1-8 were diluted with deionized water to a concentration of 10 mg / mL as sample solutions to be tested.

[0114] Test method:

[0115] (1) Preparation of test reagents:

[0116] Take 30 mg of PNPG, dissolve it in 20 mmol, pH 6.8 PBS, and make the volume to 10 mL to prepare a 10 mmol 4-Nitrophenylβ-D-glucopyranoside (PNPG) solution;

[0117] Take 50 mL of 700,000 U / mL α-glucosidase solution and dilute it 20 times with PBS at pH 6.8 to prepare 35,000 U / mL α-glucosidase solution;

[0118] Take 1.06g Na2CO3 and dissolve it in water, dilute to 10mL volumetric flask to prepare 1mol / L Na2CO3 solution.

[0119] (2) Trial grouping and intervention method:

[0120] The sample group, sample blank group, blank group and control group were set up respectively: 100 μL sample solution and 50 μL PNPG substrate solution were transferred to a 96-well ELISA plate, reacted at 37°C for 30 min, then 50 μL α-glucosidase solution was added, fully reacted at 37°C for 1 h, and finally 50 μL 1 mol / L Na2CO3 solution was added to terminate the reaction. The reagent addition method of each group is shown in Table 4.

[0121] Table 4

[0122] Reagents Blank group Control group Sample blank group Sample set PBS solution 150 100 150 100 PNPG substrate 50 50 50 50 Enzyme solution 0 50 0 50 Sodium carbonate solution 50 50 50 50

[0123] The sample was placed on an ELISA reader, the wavelength was adjusted to 405 nm, the absorbance value was measured, and the inhibition rate of the composition on α-glucosidase activity was calculated according to the following formula.

[0124] Inhibition rate (%) = [(A c -A B )-(A s -A SB )] / (A c -A B )×100%

[0125] Where: A C , represents the absorbance value of the control group; A B , represents the absorbance value of the blank group; A S , represents the absorbance value of the sample group; A SB , represents the absorbance value of the sample blank group. The results are shown in Table 5.

[0126] Table 5

[0127] Group α-glucosidase inhibition rate Example 1 68.08％ Example 2 67.89％ Example 3 68.34％ Example 4 62.90％ Example 5 61.89％ Comparative Example 1 50.78％ Comparative Example 2 57.78％ Comparative Example 3 58.67％ Comparative Example 4 56.01％ Comparative Example 5 46.88％ Comparative Example 6 52.12％ Comparative Example 7 51.19％ Comparative Example 8 54.78％

[0128] It can be seen from the data results in Table 5 that compared with Comparative Examples 1-8, the composition of the present invention selects specific raw materials to prepare a Panax notoginseng stem and leaf complex, and compoundes it with γ-aminobutyric acid, L-theanine and licorice extract. The components work synergistically to obtain an excellent α-glucosidase inhibitory effect, and has potential blood sugar lowering effect.

[0129] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0130] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0131] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition for increasing the levels of endogenous γ-aminobutyric acid and melatonin, characterized in that: The composition comprises a notoginseng stem and leaf complex, gamma-aminobutyric acid, L-theanine and liquorice extract; the notoginseng stem and leaf complex is a product obtained by alcohol extraction using notoginseng stem and leaf, gastrodia elata, tuckahoe and spiny jujube seed as raw materials.

2. The composition for increasing endogenous γ-aminobutyric acid and melatonin levels according to claim 1, characterized in that: The Panax notoginseng stem and leaf composite is prepared by a preparation method comprising the following steps: The raw materials of preparation, namely, Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds, are mixed with ethanol aqueous solution for extraction, the extraction product is filtered, and the filtrate is dried to obtain the product.

3. The composition for increasing endogenous γ-aminobutyric acid and melatonin levels according to claim 2, characterized in that: The mass ratio of the Panax notoginseng stems and leaves, Gastrodia elata, Poria cocos and Ziziphus jujuba seeds is (4-8):(1-3):(1-3):

1.

4. The composition for increasing endogenous γ-aminobutyric acid and melatonin levels according to claim 2 or 3, characterized in that: The volume fraction of the ethanol aqueous solution is 15-25%.

5. The composition for increasing endogenous γ-aminobutyric acid and melatonin levels according to any one of claims 2 to 4, characterized in that: The material-liquid ratio of the preparation raw material to the ethanol aqueous solution is 1:(15-35) g / mL; Preferably, the temperature of the leaching treatment is 15-45°C; Preferably, the extraction is performed 1-3 times, and the duration of each extraction is 20-80 hours.

6. The composition for increasing endogenous γ-aminobutyric acid and melatonin levels according to any one of claims 1 to 5, characterized in that: The composition comprises, by weight, 10-30 parts of a Panax notoginseng stem and leaf complex, 30-50 parts of gamma-aminobutyric acid, 30-50 parts of L-theanine and 0.1-1 part of a liquorice extract.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of a product having rich triterpenoid saponins and amino acid bioactive ingredients.

8. Use of the composition according to any one of claims 1 to 6 in preparing a product for improving sleep quality.

9. Use of the composition according to any one of claims 1 to 6 in the preparation of a product having antioxidant activity or α-glucosidase inhibitory activity.

10. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for increasing endogenous melatonin levels.

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