Method for preparing efficient bio-organic fertilizer by treating livestock and poultry manure with compound bacteria

Abstract

The invention discloses a method for preparing an efficient bio-organic fertilizer by treating livestock and poultry manure with compound bacteria, and belongs to the technical field of fertilizers, the method comprises the following three main steps: firstly, mixing and culturing various fermentation strains such as saccharomyces cerevisiae, bacillus licheniformis and the like according to a specific proportion to prepare a compound microbial agent; secondly, mixing the livestock manure, the organic material and the wheat flour, controlling the moisture to be 65-70%, inoculating a complex microbial inoculant, and fermenting; and finally, airing, granulating and drying the fermentation product, and adding auxiliary materials to obtain the bio-organic fertilizer. Compared with the prior art, the method disclosed by the invention not only improves the nutrient content of the fertilizer, but also enhances the biological activity of the fertilizer, has obvious effects on promoting plant growth and improving the soil structure, and realizes environment-friendly treatment and resource utilization of the livestock and poultry manure.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, and in particular to a method for preparing high-efficiency biological organic fertilizer by treating livestock and poultry excrement with composite bacteria. Background Art

[0002] With the rapid development of agriculture, the amount of manure produced by livestock and poultry farming has increased year by year. The harmless treatment and resource utilization of livestock and poultry manure has become an important issue in environmental management and sustainable agricultural development. Livestock and poultry manure contains a large amount of organic matter, nitrogen, phosphorus and other nutrients. If it is not handled properly, it will not only cause serious environmental pollution, but also waste precious agricultural resources. Therefore, converting livestock and poultry manure into high-efficiency biological organic fertilizer is of great significance for improving soil structure, increasing soil fertility and promoting crop growth.

[0003] Traditional methods for treating livestock and poultry manure include natural composting, anaerobic digestion and incineration, but these methods have problems such as long treatment cycle, low efficiency, high cost and environmental pollution. In particular, natural composting, due to its slow fermentation process, is prone to produce odor and pathogens, and has a large loss of nutrients. Although anaerobic digestion can produce biogas, its equipment investment is large and operation and maintenance are complicated. Incineration methods will release a large amount of greenhouse gases, causing secondary pollution to the environment.

[0004] In order to solve the above problems, researchers at home and abroad have carried out a lot of research work to explore new livestock and poultry manure treatment technologies. Among them, the use of microbial agents to treat livestock and poultry manure to prepare bio-organic fertilizer is an emerging technology. Its core lies in using the metabolic activities of microorganisms to accelerate the decomposition and transformation of organic matter and improve the nutritional value and environmental friendliness of fertilizers. Microbial agents can decompose organic matter in livestock and poultry manure and produce a variety of beneficial metabolites, such as enzymes, amino acids, vitamins, etc. These substances can promote plant growth and improve the disease resistance and stress resistance of crops.

[0005] Although microbial inoculant technology has shown great potential in livestock and poultry manure treatment, there are still some technical challenges. For example, how to improve the activity and stability of microbial inoculants, how to optimize the fermentation process to increase the nutrient content and biological activity of fertilizers, and how to reduce production costs and improve economic benefits. In addition, the preparation method of microbial inoculants and the selection of carrier materials are also key factors affecting their performance.

[0006] Chinese invention patent CN102219570A discloses a method for making efficient and active biological organic fertilizer using livestock and poultry manure and its preparation method. First, livestock and poultry manure, straw and peat are pretreated, and then livestock and poultry manure, straw powder and peat are mixed in a weight ratio of 3-5:1-2:1-2, and then the composite composting agent is added according to the weight ratio of the total amount of the above components: high-concentrated organic material composting agent = 5000-10000:1-2, and the composting fermentation is carried out for 7-10 days after mixing. During this period, the material needs to be stirred 1-2 times a day. The fermented and decomposed material is mixed with the functional composite bacterial agent produced by separate fermentation at a weight ratio of 5000-10000:1-2, and granulated and low-temperature dried to obtain a highly efficient and active biological organic fertilizer. The invention process is relatively simple, easy to operate, low product cost, energy saving, and waste into treasure. It is conducive to promoting a benign ecological cycle and protecting the environment, with good product quality, and significantly improving the yield and quality of agricultural products. However, the nutrient index of the biological organic fertilizer prepared by the invention and its growth-promoting effect on green vegetables still need to be improved. Summary of the invention

[0007] In view of the shortcomings of the prior art, the present invention aims to provide a method for preparing high-efficiency biological organic fertilizer by treating livestock and poultry manure with composite bacteria. The method significantly improves the nutrient content and biological activity of the biological organic fertilizer by optimizing the preparation and fermentation process of the microbial agent, while reducing the production cost, thereby achieving efficient conversion and resource utilization of livestock and poultry manure.

[0008] In order to achieve the above-mentioned object of the invention, the present invention adopts the following technical solutions:

[0009] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is as follows:

[0010] Step 1, activating, culturing, and amplifying the fermentation strains respectively until the number of effective live bacteria reaches 250-350 million / mL; adsorbing the composite bacteria on a microbial agent carrier until the number of effective live bacteria on the carrier reaches 200-300 million / g, thereby obtaining a composite microbial agent;

[0011] Step 2, the poultry and livestock manure, organic materials and wheat flour are mixed evenly, and the water content is controlled at 65-70% by Phaffia rhodozyma fermentation waste liquid to obtain a mixture, and the mixture is sent to a fermentation bin, pressurized and mixed, deodorized and sterilized, and after leaving the bin, a composite microbial agent is inoculated into the mixture, and after the materials are mixed evenly, they are piled for fermentation;

[0012] Step 3: During the fermentation process, the pile is turned over once a day and the temperature is measured once a day. After 4 to 6 days of fermentation, the pile is spread out to dry. When the moisture content drops to 28 to 32%, the fermentation product is granulated and dried. When the moisture content of the material drops below 18 to 22%, fermented fertilizer is obtained; auxiliary materials are added to the fermented fertilizer to obtain biological organic fertilizer.

[0013] The fermentation bacteria is at least one of Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus.

[0014] The composite bacteria is prepared by mixing activated saccharomyces cerevisiae, bacillus licheniformis, bacillus thuringiensis, bacillus amyloliquefaciens and lactobacillus acidophilus in a mass ratio of 2-4:0.5-2:1-3:0.5-2:2-4.

[0015] The Phaffia rhodozyma fermentation waste liquid contains 2-4 g / L glucose, 0.5-2 g / L yeast extract, 0.4-0.6 g / L peptone, 0.01-0.03 g / L CaCl2·2H2O, 0.1-0.3 g / L MgSO4·7H2O, 0.05-0.2 g / L pyruvic acid, 0.1-0.2 g / L citric acid, 0.005-0.002 g / L α-ketoglutaric acid, 0.1-0.3 g / L malic acid, 2-4 g / L lactic acid and 0.4-0.6 g / L Phaffia rhodozyma.

[0016] The livestock manure is a mixture of pig manure, chicken manure and cow manure in a mass ratio of 4-6:0.5-2:2-4.

[0017] 70-90 kg of organic materials, 1-2 kg of wheat flour and 0.8-1.2 kg of composite microbial agent are added to every cubic meter of livestock manure.

[0018] The organic material is a mixture of rice husk, sawdust and soybean meal in a mass ratio of 6-8:1-3:0.5-2.

[0019] The auxiliary materials are bran, fish meal, cottonseed cake, peat and bentonite in a mass ratio of 1-3:0.5-2:0.5-2:2-4:1-3.

[0020] The mass ratio of the fermentation fertilizer to the auxiliary material is 6-8:2-4.

[0021] The preparation method of the microbial agent carrier is as follows, in parts by weight:

[0022] S1: adding 0.5 to 2 parts of bagasse to 40 to 60 parts of an aqueous solution containing 0.3 to 0.5 parts of sodium hypochlorite and 0.1 to 0.3 parts of cysteine, treating at 75 to 85° C. for 3 to 5 hours, washing with water until neutral after the treatment, and drying to obtain a pretreated product;

[0023] S2: adding the pre-treated product prepared in step S1 to 60-80 parts of water, adding 0.2-0.4 parts of hydrogen peroxide at 50-70° C., and then adding 0.001-0.003 parts of 97.8-98.2 wt % concentrated sulfuric acid, 0.3-0.5 parts of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.4-0.6 parts of 2-(2-aminobut-3-enyl)malonic acid, treating for 4-6 hours, washing with water and anhydrous ethanol for 1-3 times respectively, and drying to obtain a post-treated product;

[0024] S3: The post-treated product prepared in step S2, 0.05-0.2 parts of bentonite and 40-60 parts of polystyrene are mixed uniformly, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0025] The functions of each substance in the present invention are as follows:

[0026] Fermentation strains (Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus) are used to prepare composite microbial agents, which play a key role in the decomposition and transformation of livestock and poultry manure, promote the decomposition of organic matter, produce beneficial metabolites, and improve the nutritional value of fertilizers and soil improvement effects.

[0027] Microbial agent carriers are used to adsorb and fix composite bacteria, ensuring the uniform distribution and activity of the agent during the fermentation process and improving the stability and durability of the agent.

[0028] Poultry and livestock manure (pig manure, chicken manure, cow manure) is the main raw material of biological organic fertilizer, providing rich organic matter and nutrients, and is the matrix for the growth and reproduction of microorganisms.

[0029] Organic materials (rice husks, sawdust, soybean meal) increase the looseness of the material, improve aeration, provide additional carbon sources and nutrients, and promote microbial activity.

[0030] Wheat flour provides an easily degradable carbon source, promoting the rapid proliferation of microorganisms.

[0031] Phaffia rhodozyma fermentation waste liquid serves as a moisture regulator and contains a variety of nutrients and metabolites, which improves fermentation efficiency and the nutrient content of organic fertilizer.

[0032] Auxiliary materials (bran, fish meal, cottonseed cake, peat, bentonite) improve the physical properties of fertilizers, increase nutrients, improve the water retention and adsorption of fertilizers, and improve the application effect of fertilizers.

[0033] As one of the raw materials for microbial agent carriers, sugarcane bagasse provides organic substances such as cellulose to increase the mechanical strength and adsorption capacity of the carrier.

[0034] Sodium hypochlorite and cysteine ​​are used for bleaching and disinfection during the pretreatment of bagasse to remove impurities and harmful microorganisms.

[0035] Hydrogen peroxide and concentrated sulfuric acid are used for oxidation treatment of bagasse to increase its reactivity.

[0036] 1,3,5-Triacryloylhexahydro-1,3,5-triazine and 2-(2-aminobut-3-enyl)malonic acid are used for the modification of bagasse to increase its crosslinking degree and stability and improve the performance of the carrier.

[0037] Polystyrene, as the main component of the microbial agent carrier, provides the basic structure and shape of the carrier.

[0038] The combination and interaction of these substances enable the present invention to efficiently convert livestock and poultry manure into nutrient-rich biological organic fertilizer, while improving the biological activity and environmental friendliness of the fertilizer.

[0039] Compared with the prior art, it has the following beneficial effects:

[0040] 1) The present invention uses a specific composite bacterial strain and a fermentation process to prepare a biological organic fertilizer with a higher nutrient index and can more effectively promote plant growth.

[0041] 2) The present invention utilizes Phaffia rhodozyma fermentation waste liquid as a moisture regulator, thereby realizing the reuse of industrial waste liquid, reducing environmental pollution, saving water resources, realizing resource utilization of waste, and having good environmental benefits.

[0042] 3) The composite microbial agent used in the present invention contains a variety of beneficial microorganisms, which can improve the biological activity of organic fertilizers and enhance the decomposition ability of fertilizers during the fermentation process, thereby more effectively converting organic waste into nutrients that can be used by plants, thereby improving the nutritional value of fertilizers and the soil improvement effect. DETAILED DESCRIPTION

[0043] Main sources of substances:

[0044] Bagasse, water content of about 52%, bagasse fiber length: 2-15mm; main components: lignin 18-22%, cellulose 40-50%, hemicellulose 25-30%, protein 2.5%, crude ash 2.5%, pectin 1.5%, fat, sugar 1.5-2.5%. Article number: GZZ12, Guangxi Yuante Agricultural Technology Co., Ltd.

[0045] Saccharomyces cerevisiae, commercially available product, strain number: CICC 30117, Latin name: Saccharomyces cerevisiae, China Industrial Microbiological Culture Collection Management Center.

[0046] Bacillus licheniformis, commercially available product, strain number: CICC 10291, Latin name: Bacilluslicheniformis, China Industrial Microbiological Culture Collection Management Center.

[0047] Bacillus thuringiensis, commercially available product, strain number: CICC 20553, Latin name: Bacillus thuringiensis, China Industrial Microbiological Culture Collection Management Center.

[0048] Bacillus amyloliquefaciens, commercially available product, strain number: CICC 20198, Latin name Bacillus amyloliquefaciens, China Industrial Microbiology Culture Collection Management Center.

[0049] Lactobacillus acidophilus, commercially available product, strain number: CICC 6081, Latin name: Lactobacillus acidophilus, China Industrial Microbiology Culture Collection Management Center.

[0050] Phaffia rhodozyma, a commercial product, strain number CICC 33064, Latin name: Phaffia rhodozyma, China Industrial Microbiological Culture Collection Management Center.

[0051] Bentonite, particle size: 400 mesh, density: 2.3g / cm 3 .

[0052] Polystyrene, brand: 158K, processing level: spraying, injection molding grade, manufacturer: BASF-Yanzhou.

[0053] The remaining raw materials in the examples and comparative examples of the present invention are all commercially available products.

[0054] The design idea of ​​the present invention is to use specific composite bacteria to prepare highly active composite microbial agents through activation, cultivation and amplification, and then use agricultural by-products such as bagasse as basic materials to prepare a new type of microbial agent carrier through chemical treatment and physical processing methods. The carrier can effectively adsorb and stabilize the composite microbial agent, improve the biological activity and nutrient release efficiency of the fertilizer, and apply it to the treatment process of livestock and poultry manure to promote the decomposition and conversion of organic matter in the manure. At the same time, the fermentation waste liquid of Phaffia rhodozyma is used as a moisture regulator to improve the nutrient content and biological activity of the organic fertilizer. Finally, a high-efficiency biological organic fertilizer is prepared through fermentation and granulation processes, aiming to improve the environmental performance, economic benefits and sustainability of agricultural production of the fertilizer.

[0055] Example 1

[0056] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is as follows:

[0057] Step 1, activating, culturing and amplifying Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus respectively until the number of effective live bacteria of each reaches 300 million / mL; adsorbing the composite bacteria on a microbial agent carrier until the number of effective live bacteria on the carrier is 250 million / g, wherein the composite bacteria is obtained by mixing the activated Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus in a mass ratio of 3:1:2:1:3 to obtain a composite microbial agent;

[0058] Step 2, uniformly mix livestock manure, organic materials and wheat flour, wherein the livestock manure is a mixture of pig manure, chicken manure and cow manure in a mass ratio of 5:1:3, and the organic materials are a mixture of rice husk, sawdust and soybean meal in a mass ratio of 7:2:1, and the water content is controlled at 68% by using Phaffia rhodozyma fermentation waste liquid to obtain a mixture, and the mixture is sent to a fermentation bin, pressurized and mixed, deodorized and sterilized, and after leaving the bin, a composite microbial agent is inoculated into the mixture, 80 kg of organic materials, 1.5 kg of wheat flour and 1 kg of composite microbial agent are added to each cubic meter of livestock manure, and the materials are uniformly mixed and then piled for fermentation;

[0059] Step 3: During the fermentation process, the pile is turned over once a day, and the temperature is measured once a day. After 5 days of fermentation, the pile is spread out to dry. When the moisture content drops to 30%, the fermentation product is granulated and dried. When the moisture content of the material drops below 20%, fermented fertilizer is obtained. Auxiliary materials are added to the fermented fertilizer, and the mass ratio of the fermented fertilizer to the auxiliary materials is 7:3. The auxiliary materials are bran, fish meal, cottonseed cake, peat, and bentonite in a mass ratio of 2:1:1:3:2 to obtain biological organic fertilizer.

[0060] The Phaffia rhodozyma fermentation waste liquid contains 3g / L glucose, 1g / L yeast extract, 0.5g / L peptone, 0.02g / L CaCl2·2H2O, 0.2g / LMgSO4·7H2O, 0.1g / L pyruvic acid, 0.15g / L citric acid, 0.01g / L α-ketoglutaric acid, 0.2g / L malic acid, 3g / L lactic acid and 0.5g / L Phaffia rhodozyma.

[0061] The preparation method of the microbial agent carrier is as follows:

[0062] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0063] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., and then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.5 kg of 2-(2-aminobut-3-enyl)malonic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0064] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0065] Example 2

[0066] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0067] The preparation method of the microbial agent carrier is as follows:

[0068] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0069] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt % concentrated sulfuric acid, 0.4 kg of di[(1-oxo-2-propenyl)amino]-acetic acid and 0.5 kg of 2-(2-aminobut-3-enyl)malonic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0070] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0071] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0072] Example 3

[0073] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0074] The preparation method of the microbial agent carrier is as follows:

[0075] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0076] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of N,N-methylenebis(methylpropenylamine) and 0.5 kg of 2-(2-aminobut-3-enyl)malonic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0077] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0078] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0079] Example 4

[0080] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0081] The preparation method of the microbial agent carrier is as follows:

[0082] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0083] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.5 kg of 4-amino-2-cyclopentene-1-carboxylic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0084] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0085] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0086] Example 5

[0087] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0088] The preparation method of the microbial agent carrier is as follows:

[0089] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0090] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.5 kg of 2-amino-5-hexenoic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0091] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0092] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0093] Comparative Example 1

[0094] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0095] The preparation method of the microbial agent carrier is as follows:

[0096] S1: 1 kg of corn cob was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the corn cob was rinsed with water until neutral, and dried to obtain a pretreated product;

[0097] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., and then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.5 kg of 2-(2-aminobut-3-enyl)malonic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0098] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0099] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0100] Comparative Example 2

[0101] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0102] The preparation method of the microbial agent carrier is as follows:

[0103] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0104] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt% concentrated sulfuric acid, 0.4 kg of N,N-methylenebisacrylamide and 0.5 kg of 2-(2-aminobut-3-enyl)malonic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0105] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0106] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0107] Comparative Example 3

[0108] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0109] The preparation method of the microbial agent carrier is as follows:

[0110] S1: 1 kg of bagasse was added into 50 kg of aqueous solution containing 0.4 kg of sodium hypochlorite and 0.2 kg of cysteine, and treated at 80° C. for 4 hours. After the treatment, the mixture was rinsed with water until neutral, and dried to obtain a pretreated product;

[0111] S2: adding the pre-treated product prepared in step S1 to 70 kg of water, adding 0.3 kg of hydrogen peroxide at 60° C., then adding 0.002 kg of 98 wt % concentrated sulfuric acid, 0.4 kg of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.5 kg of aminohexanoic acid, treating for 5 hours, washing with water and anhydrous ethanol for 3 times respectively, and drying to obtain a post-treated product;

[0112] S3: The post-treated product prepared in step S2, 0.1 kg of bentonite and 50 kg of polystyrene are mixed, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0113] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0114] Comparative Example 4

[0115] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is basically the same as that in Example 1, the only difference being that the preparation method of the microbial agent carrier is different.

[0116] The preparation method of the microbial agent carrier is as follows:

[0117] 1 kg of bagasse, 0.1 kg of bentonite and 50 kg of polystyrene were mixed and extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

[0118] The Phaffia rhodozyma fermentation waste liquid is the same as that in Example 1.

[0119] Comparative Example 5

[0120] A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria is as follows:

[0121] Step 1, activating, culturing and amplifying Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus respectively until the number of effective live bacteria of each reaches 300 million / mL; adsorbing the composite bacteria on a microbial agent carrier until the number of effective live bacteria on the carrier is 250 million / g, wherein the composite bacteria is obtained by mixing the activated Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus in a mass ratio of 3:1:2:1:3 to obtain a composite microbial agent;

[0122] Step 2, the livestock manure, organic material and wheat flour are mixed evenly, 80kg of organic material, 1.5kg of wheat flour and 1kg of composite microbial agent are added to each cubic meter of livestock manure, the livestock manure is a mixture of pig manure, chicken manure and cow manure in a mass ratio of 5:1:3, the organic material is a mixture of rice husk, sawdust and soybean meal in a mass ratio of 7:2:1, water is used to control the moisture at 68%, and a mixture is obtained, the mixture is sent to a fermentation bin, pressurized and mixed, deodorized and sterilized, and after leaving the bin, the composite microbial agent is inoculated into the mixture, and after the materials are mixed evenly, they are piled for fermentation;

[0123] Step 3: During the fermentation process, the pile is turned over once a day, and the temperature is measured once a day. After 5 days of fermentation, the pile is spread out to dry. When the moisture content drops to 30%, the fermentation product is granulated and dried. When the moisture content of the material drops below 20%, fermented fertilizer is obtained. Auxiliary materials are added to the fermented fertilizer, and the mass ratio of the fermented fertilizer to the auxiliary materials is 7:3. The auxiliary materials are bran, fish meal, cottonseed cake, peat, and bentonite in a mass ratio of 2:1:1:3:2 to obtain biological organic fertilizer.

[0124] The preparation method of the microbial agent carrier is the same as that in Example 1.

[0125] Test Example 1

[0126] Nutrient index test

[0127] The bio-organic fertilizer prepared by the present invention was subjected to conventional nutrient index determination of organic fertilizer according to the bio-organic fertilizer standard "NY525-2012". Among them, the nitrogen content was determined by the Kjeldahl method; the phosphorus (P2O5) and potassium (K2O) content were determined by the standard curve method. The technical index results of the bio-organic fertilizer are shown in Table 1.

[0128] Table 1

[0129]

[0130]

[0131] Test Example 2

[0132] Field experiments

[0133] A field experiment for evaluating the effect of the bio-organic fertilizer prepared by the present invention was carried out in Luotian County, Huanggang, Hubei Province. The experiment set up 10 treatment groups and 1 control group, which were respectively the bio-organic fertilizers prepared by the embodiments of the present invention and the comparative examples, and the control group was not fertilized but the same amount of water was applied; three replicates were set for each treatment.

[0134] After leveling the land, evenly plant the cabbage seeds in the soil, water and cover the soil. Apply bio-organic fertilizer once after 10 days, and then apply bio-organic fertilizer again after 1 week. The amount of fertilizer applied each time is 1.5kg / m2 , and bio-organic fertilizer was applied 3 times. The cabbages were harvested after maturity, and a 1m×1m area was selected for sampling in each treatment. The weight of each plant was calculated by weighing, and the average value was shown in Table 2.

[0135] Table 2

[0136] Experimental protocol Single plant weight / g Example 1 728.3 Example 2 716.2 Example 3 689.4 Example 4 714.9 Example 5 711.3 Comparative Example 1 683.0 Comparative Example 2 693.6 Comparative Example 3 701.4 Comparative Example 4 685.4 Comparative Example 5 693.6 Control group 557.2

[0137] It can be seen from the data in Tables 1 and 2 that the biological organic fertilizer prepared in Example 1 has the best nutrient index and has a high promoting effect on the growth of Chinese cabbage.

[0138] The 1,3,5-triacryloylhexahydro-1,3,5-triazine used in Example 1 shows better nutrient indicators and a promoting effect on the growth of pakchoi compared to the different compounds used in other examples and comparative examples, which may be related to the unique properties of 1,3,5-triacryloylhexahydro-1,3,5-triazine. 1,3,5-triacryloylhexahydro-1,3,5-triazine is a multifunctional monomer with three acryloyl groups, which makes it more active and efficient during crosslinking and curing. This compound also has good biocompatibility. In addition, the multifunctional properties of 1,3,5-triacryloylhexahydro-1,3,5-triazine may help to improve the mechanical strength and stability of the microbial agent carrier, thereby better maintaining the microbial activity and the reasonable release of nutrients during microbial fermentation and organic fertilizer preparation, which may be the reason for its better performance in nutrient indicators and plant growth promotion.

[0139] 2-(2-aminobutan-3-enyl) propanedioic acid used in Example 1 has better nutrient index and the promotion effect to the growth of Chinese cabbage compared with 4-amino-2-cyclopentene-1-carboxylic acid, 2-amino-5-hexenoic acid and aminohexatin acid used in other embodiments and comparative examples. This may be because 2-(2-aminobutan-3-enyl) propanedioic acid, as an organic compound, has specific biological activity and functional characteristics, and these characteristics may contribute to improving the nutrient retention capacity and release efficiency of the microbial agent carrier. In addition, 2-(2-aminobutan-3-enyl) propanedioic acid may have a promotion effect on microbial activity, thereby enhancing the effectiveness of microbial agent, improving the biological activity of organic fertilizer and the promotion effect of plant growth. In contrast, other compounds may not be as obvious as 2-(2-aminobutan-3-enyl) propanedioic acid in these aspects, causing the biological organic fertilizer of Example 1 to perform better in nutrient index and plant growth promotion.

[0140] The bagasse used in embodiment 1 is rich in nutrients as an agricultural byproduct, containing high organic matter and various trace elements, which are conducive to the growth and reproduction of microorganisms. The high carbon-nitrogen ratio (C / N) in bagasse is conducive to the metabolic activity of microorganisms and promotes the conversion and release of nutrients in organic fertilizers. In addition, the physical structure of bagasse is relatively loose, which is conducive to the maintenance of air and moisture, and provides a good growth environment for microorganisms. In contrast, although corncob also contains a certain amount of organic matter and nutrients, its nutrients and structural characteristics may not be as good as bagasse. The carbon-nitrogen ratio of corncob is relatively low, which may cause the fixation and release efficiency of nitrogen to be not as good as bagasse in the microbial metabolism process. In addition, the structure of corncob is relatively tight, which may affect the penetration and growth of microorganisms, thereby affecting the nutrient release and plant absorption efficiency of organic fertilizers.

[0141] The Phaffia rhodozyma fermentation waste liquid used in Example 1 showed better nutrient indicators and a promoting effect on the growth of Chinese cabbage in the preparation of biological organic fertilizer compared to the ordinary water used in Comparative Example 5. This may be related to the rich nutrients and microbial activity in the Phaffia rhodozyma fermentation waste liquid. The Phaffia rhodozyma fermentation waste liquid contains organic substances such as glucose, yeast extract powder, and peptone, as well as inorganic salts such as calcium and magnesium. These components provide the microorganisms with the nutrients required for growth and promote the activity and metabolism of the microorganisms. In addition, intermediate metabolites such as pyruvic acid, citric acid, α-ketoglutaric acid, malic acid, and lactic acid in the fermentation waste liquid may have a direct or indirect promoting effect on plant growth, such as providing additional carbon sources and energy, improving the pH value and structure of the soil, and stimulating the growth of plant roots. As a resource rich in nutrients and biological activity, the waste liquid from Phaffia rhodozyma fermentation can increase the nutrients required for the growth and reproduction of thermophilic microorganisms and reduce the input of some raw materials; it also reduces a large part of the input of fermentation water and protects our precious water resources; achieving zero discharge of the waste liquid from Phaffia rhodozyma fermentation not only saves the cost of sewage treatment, but also protects the ecological environment.

Claims

1. A method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria, characterized in that: Here’s how: Step 1, activating, culturing, and amplifying the fermentation strains respectively until the number of effective live bacteria reaches 250-350 million / mL; adsorbing the composite bacteria on a microbial agent carrier until the number of effective live bacteria on the carrier reaches 200-300 million / g, thereby obtaining a composite microbial agent; Step 2, the poultry and livestock manure, organic materials and wheat flour are mixed evenly, and the water content is controlled at 65-70% by Phaffia rhodozyma fermentation waste liquid to obtain a mixture, and the mixture is sent to a fermentation bin, pressurized and mixed, deodorized and sterilized, and after leaving the bin, a composite microbial agent is inoculated into the mixture, and after the materials are mixed evenly, they are piled for fermentation; Step 3: During the fermentation process, the pile is turned over once a day and the temperature is measured once a day. After 4 to 6 days of fermentation, the pile is spread out to dry. When the moisture content drops to 28 to 32%, the fermentation product is granulated and dried. When the moisture content of the material drops below 18 to 22%, fermented fertilizer is obtained; auxiliary materials are added to the fermented fertilizer to obtain biological organic fertilizer.

2. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria as claimed in claim 1, characterized in that: The fermentation bacteria is at least one of Saccharomyces cerevisiae, Bacillus licheniformis, Bacillus thuringiensis, Bacillus amyloliquefaciens and Lactobacillus acidophilus.

3. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria as claimed in claim 1, characterized in that: The composite bacteria is prepared by mixing activated saccharomyces cerevisiae, bacillus licheniformis, bacillus thuringiensis, bacillus amyloliquefaciens and lactobacillus acidophilus in a mass ratio of 2-4:0.5-2:1-3:0.5-2:2-4.

4. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria as claimed in claim 1, characterized in that: The Phaffia rhodozyma fermentation waste liquid contains 2-4 g / L glucose, 0.5-2 g / L yeast extract, 0.4-0.6 g / L peptone, 0.01-0.03 g / L CaCl2·2H2O, 0.1-0.3 g / L MgSO4·7H2O, 0.05-0.2 g / L pyruvic acid, 0.1-0.2 g / L citric acid, 0.005-0.002 g / L α-ketoglutaric acid, 0.1-0.3 g / L malic acid, 2-4 g / L lactic acid and 0.4-0.6 g / L Phaffia rhodozyma.

5. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria as claimed in claim 1, characterized in that: The livestock manure is a mixture of pig manure, chicken manure and cow manure in a mass ratio of 4-6:0.5-2:2-4.

6. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry excrement with composite bacteria as claimed in claim 1, characterized in that: 70-90 kg of organic materials, 1-2 kg of wheat flour and 0.8-1.2 kg of composite microbial agent are added to every cubic meter of livestock manure.

7. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry excrement with composite bacteria as claimed in claim 1, characterized in that: The organic material is a mixture of rice husk, sawdust and soybean meal in a mass ratio of 6-8:1-3:0.5-2.

8. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry manure with composite bacteria as claimed in claim 1, characterized in that: The auxiliary materials are bran, fish meal, cottonseed cake, peat and bentonite in a mass ratio of 1-3:0.5-2:0.5-2:2-4:1-3.

9. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry excrement with composite bacteria as claimed in claim 1, characterized in that: The mass ratio of the fermentation fertilizer to the auxiliary material is 6-8:2-4.

10. The method for preparing high-efficiency bio-organic fertilizer by treating livestock and poultry excrement with composite bacteria as claimed in claim 1, characterized in that: The preparation method of the microbial agent carrier is as follows, in parts by weight: S1: adding 0.5 to 2 parts of bagasse to 40 to 60 parts of an aqueous solution containing 0.3 to 0.5 parts of sodium hypochlorite and 0.1 to 0.3 parts of cysteine, treating at 75 to 85° C. for 3 to 5 hours, washing with water until neutral after the treatment, and drying to obtain a pretreated product; S2: adding the pre-treated product prepared in step S1 to 60-80 parts of water, adding 0.2-0.4 parts of hydrogen peroxide at 50-70° C., and then adding 0.001-0.003 parts of 97.8-98.2 wt % concentrated sulfuric acid, 0.3-0.5 parts of 1,3,5-triacryloylhexahydro-1,3,5-triazine and 0.4-0.6 parts of 2-(2-aminobut-3-enyl)malonic acid, treating for 4-6 hours, washing with water and anhydrous ethanol for 1-3 times respectively, and drying to obtain a post-treated product; S3: The post-treated product prepared in step S2, 0.05-0.2 parts of bentonite and 40-60 parts of polystyrene are mixed uniformly, and the mixture is extruded through a screw extruder using a conventional extrusion process to obtain a microbial agent carrier.

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