Fungal protein composite liquid and its preparation method and application

Abstract

The present invention discloses a fungal protein composite liquid and a preparation method thereof, and the application of the fungal protein composite liquid in emulsifying alkanes. The fungal protein composite liquid is prepared from the following raw materials by mass: fungal protein, inorganic compound, buffer salt, polyol compound, auxiliary agent, thickener and water. The fungal protein, inorganic compound, buffer salt and polyol compound are mixed and subjected to ultrasound to obtain solution A; the auxiliary agent, thickener and deionized water are mixed and subjected to vortex oscillation to obtain solution B; and solution A and solution B are mixed to obtain the fungal protein composite liquid. The emulsified alkane emulsion of the present invention has high-efficiency dispersibility, is uniform and not easy to stratify, and achieves a higher emulsification effect at a lower emulsifier concentration.

Description

Technical Field

[0001] The invention belongs to the technical field of emulsions, and specifically relates to a fungal protein composite liquid and a preparation method and application thereof. Background Art

[0002] Alkane emulsification is widely used in the fields of petrochemicals, pesticide preparations, daily chemicals and environmental protection. It helps to mix oil and water, improve performance, increase product stability, and plays an important role in pollution control. Methods for emulsifying alkanes include surfactant emulsification, high shear force emulsification, ultrasonic emulsification, etc. Surfactants have obvious advantages in improving the stability and uniformity of emulsions in the application field of alkane emulsification. However, with the deepening of research, the problems of difficulty in selecting alkane emulsifiers, high process requirements, and possible toxicity to the environment and organisms are constantly exposed. Therefore, it is urgent to develop a safe, environmentally friendly and efficient alkane emulsification method. Summary of the invention

[0003] The present invention aims to provide a stable, efficient, biological and environmentally friendly fungal protein composite liquid and a preparation method and application thereof.

[0004] The technical solution of the present invention is: a fungal protein composite liquid, which is composed of the following components by mass: 0.5-2 parts of fungal protein, 2-5 parts of inorganic compounds, 2-5 parts of buffer salts, 2-5 parts of polyol compounds, 0.5-1 parts of thickeners, 0.5-1 parts of auxiliary agents and 300-400 parts of deionized water;

[0005] The inorganic compound is any one of sodium chloride and potassium chloride;

[0006] The buffer salt is any one of phosphate, triethanolamine dodecyl sulfate, and sodium N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid;

[0007] The polyol compound is any one of glycerol, polyethylene glycol, diethylene glycol, and sorbitol;

[0008] The thickener is any one of polyethylene oxide, polyacrylamide, lecithin, sodium lauryl sulfate, monopalmitoyl glycerol, and dipalmitoyl glycerol;

[0009] The auxiliary agent is any one of maleic anhydride, lysine, epoxy resin, 1,4-dioxane and methacrylic acid.

[0010] The present invention also provides a method for preparing the fungal protein composite liquid, comprising the following steps:

[0011] First, fungal protein, inorganic compound, buffer salt, polyol compound and deionized water were mixed according to the following weight parts, and solution A was obtained after ultrasonic treatment:

[0012] 0.5-2 parts of the fungal protein, 2-5 parts of the inorganic compound, 2-5 parts of the buffer salt, 2-5 parts of the polyol compound, and 100-150 parts of deionized water;

[0013] Next, the thickener, additive and deionized water were mixed and filtered to obtain solution B:

[0014] 0.5-1 part of the thickener, 0.5-1 part of the auxiliary agent and 200-250 parts of deionized water;

[0015] Finally, solution A and solution B are mixed to obtain a fungal protein composite solution.

[0016] The present invention also provides the use of the fungal protein complex liquid prepared by the above method as a biological surfactant in emulsifying alkanes.

[0017] The use of the fungal protein complex liquid as a biosurfactant in emulsifying alkanes comprises the following steps: adjusting the pH of the fungal protein complex liquid to 4-8, filtering and sterilizing the alkanes, mixing the alkanes, i.e., the oil phase, with the water phase, adding the fungal protein complex liquid, emulsifying by magnetic stirring and vortex oscillation, and finally degassing by ultrasonication;

[0018] The volume ratio of the water phase to the oil phase is 1:1-55;

[0019] The mass ratio of the fungal protein complex liquid to the alkane is 1:0.5-2;

[0020] The alkane is a C5-C16 liquid alkane.

[0021] Furthermore, the alkane filtration pore size range is 0.45-1 μm,

[0022] Furthermore, the magnetic stirring conditions are 500-1000 rpm, 3-5 h, and the vortex oscillation time is 0.5-1 h.

[0023] Furthermore, the ultrasonic temperature is controlled at 5-30°C, and the ultrasonic time is 12-48h.

[0024] Beneficial Effects

[0025] The fungal protein used in the present invention is a type of small molecular weight protein secreted by higher filamentous fungi during a specific physiological period. Through research and analysis, it is found that this type of protein has a high surface activity and can form an amphiphilic protein film at the interface of the two phases through self-assembly, thereby changing the hydrophilicity / hydrophobicity of the original medium surface. Due to its special physicochemical properties and amphiphilicity, this type of protein makes hydrophobic proteins, like surfactants, enhance the hydrophilicity of hydrophobic surfaces and enhance the hydrophobicity of hydrophilic surfaces.

[0026] The inorganic salt, buffer salt and polyol compound used in the present invention have the function of improving the stability and solubility of fungal protein, and the thickener and auxiliary agent help to enhance the stability of the emulsification process.

[0027] The alkane emulsion emulsified with the fungal protein composite liquid prepared by the present invention has high-efficiency dispersibility, is uniform and not easy to be stratified, and achieves a higher emulsification effect at a lower emulsifier concentration. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 Example 1 Emulsified hexadecane phenomenon;

[0029] Figure 2 Example 2 Emulsified decane phenomenon;

[0030] Figure 3 Example 3 Emulsified pentane phenomenon;

[0031] Figure 4 Comparative Example 1 has no biosurfactant and emulsifies hexadecane;

[0032] Figure 5 In Comparative Example 11, only the fungal protein emulsified hexadecane. DETAILED DESCRIPTION

[0033] The present invention will be further described below by way of specific embodiments.

[0034] The fungal protein used in this application is obtained according to the disclosed technology of CN 108060169 A patent.

[0035] Example 1

[0036] The method for preparing the fungal protein composite liquid comprises the following steps:

[0037] 2 parts of fungal protein, 4 parts of sodium chloride, 4 parts of phosphate, 4 parts of glycerol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath was ultrasonicated.

[0038] 1 part of maleic anhydride, 1 part of polyethylene oxide and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the solution was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0039] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0040] The fungal protein complex liquid is used as a biosurfactant in emulsifying hexadecane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 8, filtering and sterilizing the hexadecane through a 1 μm pore size filter membrane, mixing water (aqueous phase) and hexadecane (oil phase) at a volume ratio of 1:2.5, adding 2 portions of the fungal protein complex liquid, magnetically stirring at 1000 rpm for 5 hours, vortexing for 3 hours, controlling the temperature at 5-30°C, and ultrasonicating for 48 hours.

[0041] Example 2

[0042] The method for preparing the fungal protein composite liquid comprises the following steps:

[0043] 1 part of fungal protein, 3 parts of sodium chloride, 3 parts of triethanolamine dodecyl sulfate, 3 parts of polyethylene glycol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A, which was specifically mixed by pipetting and ultrasonicating in an ice bath.

[0044] 0.7 parts of lysine, 0.7 parts of sodium dodecyl sulfate and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the solution was mixed by vortex shaking and filtered through a 1-10 μm pore size filter membrane.

[0045] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0046] The fungal protein complex liquid is used as a biosurfactant in emulsifying decan, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 7, filtering and sterilizing with a decan 1 μm pore size filter membrane, mixing water (aqueous phase) and decan (oil phase) at a volume ratio of 1:1.5, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 800 rpm for 4 hours, vortexing for 2 hours, controlling the temperature at 5-30°C, and ultrasonicating for 24 hours.

[0047] Example 3

[0048] The method for preparing the fungal protein composite liquid comprises the following steps:

[0049] 0.5 parts of fungal protein, 2 parts of potassium chloride, 2 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, 2 parts of diethylene glycol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0050] 0.5 parts of methacrylic acid, 0.5 parts of dipalmitoylglycerol and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the mixture was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0051] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0052] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 5, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and pentane (oil phase) in a volume ratio of 1:1, adding 0.5 parts of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0053] Example 4

[0054] The method for preparing the fungal protein composite liquid comprises the following steps:

[0055] 1.5 parts of fungal protein, 4 parts of potassium chloride, 3 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, 3 parts of sorbitol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0056] 0.6 parts of epoxy resin, 0.7 parts of polyacrylamide and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the mixture was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0057] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0058] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 4, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and hexane (oil phase) in a volume ratio of 1:10, adding 2 portions of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0059] Example 5

[0060] The method for preparing the fungal protein composite liquid comprises the following steps:

[0061] 1 part of fungal protein, 5 parts of sodium chloride, 5 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, 5 parts of sorbitol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0062] 1 part of 1,4-dioxane, 0.8 part of dipalmitoylglycerol and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the mixture was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0063] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0064] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 5, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and heptane (oil phase) in a volume ratio of 1:20, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0065] Example 6

[0066] The method for preparing the fungal protein composite liquid comprises the following steps:

[0067] 2 parts of fungal protein, 4 parts of potassium chloride, 2 parts of triethanolamine dodecyl sulfate, 5 parts of diethylene glycol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0068] 0.7 parts of lysine, 0.8 parts of lecithin and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the solution was mixed by vortex shaking and filtered through a 1-10 μm pore size filter membrane.

[0069] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0070] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 4, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and octane (oil phase) in a volume ratio of 1:30, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0071] Example 7

[0072] The method for preparing the fungal protein composite liquid comprises the following steps:

[0073] 0.5 parts of fungal protein, 1 part of sodium chloride, 2 parts of phosphate, 5 parts of phosphate and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0074] 0.5 parts of maleic anhydride, 0.6 parts of sodium dodecyl sulfate and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the solution was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0075] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0076] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 7, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and dodecapentane (oil phase) in a volume ratio of 1:30, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0077] Example 8

[0078] The method for preparing the fungal protein composite liquid comprises the following steps:

[0079] 2 parts of fungal protein, 3 parts of potassium chloride, 3 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, 4 parts of diethylene glycol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0080] 0.5 parts of methacrylic acid, 0.5 parts of monopalmitoylglycerol and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the mixture was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0081] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0082] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 4, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and tetradecane (oil phase) in a volume ratio of 1:40, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0083] Example 9

[0084] The method for preparing the fungal protein composite liquid comprises the following steps:

[0085] The preparation method of a composite liquid of 0.5 parts of fungal protein, 2 parts of potassium chloride, 2 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, and 2 parts of fungal protein comprises the following steps:

[0086] 2 parts of fungal protein, 3 parts of potassium chloride, 5 parts of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium salt, 5 parts of diethylene glycol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0087] 1 part of lysine, 1 part of lecithin and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the solution was mixed by vortex shaking and filtered by a 1-10 μm pore size filter membrane.

[0088] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0089] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 6, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and hexadecane (oil phase) in a volume ratio of 1:40, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0090] Example 10

[0091] The method for preparing the fungal protein composite liquid comprises the following steps:

[0092] 2 parts of fungal protein, 5 parts of potassium chloride, 5 parts of triethanolamine lauryl sulfate, 5 parts of sorbitol and 100 parts of deionized water were mixed and ultrasonicated to obtain solution A. Specifically, a pipette was used for mixing and an ice bath ultrasonication was performed.

[0093] 1 part of epoxy resin, 1 part of polyethylene oxide and 200 parts of deionized water were mixed and filtered to obtain solution B. Specifically, the mixture was mixed by vortex shaking and filtered through a filter membrane with a pore size of 1-10 μm.

[0094] The solution A and the solution B are mixed to obtain a fungal protein complex solution.

[0095] The fungal protein complex liquid is used as a biosurfactant in emulsified pentane, comprising the following steps: adjusting the pH of the fungal protein complex liquid to 4, filtering and sterilizing with a pentane 1 μm pore size filter membrane, mixing water (aqueous phase) and hexadecane (oil phase) in a volume ratio of 1:55, adding 1 portion of the fungal protein complex liquid, magnetically stirring at 500 rpm for 3 hours, vortexing for 1 hour, controlling the temperature at 5-30°C, and ultrasonicating for 12 hours.

[0096] Comparative Example 1

[0097] Compared with Example 1, the number of the fungal protein composite liquid is 0. Without a biosurfactant, an amphoteric protein film cannot be formed at the oil-water interface, and hexadecane has no emulsifying effect.

[0098] Comparative Example 2

[0099] Compared with Example 1, the amount of the fungal protein composite liquid is 0.1 part. The concentration of the fungal protein composite liquid in the total system is too low, and the amphoteric protein film is insufficient to form at the interface between the oil and water phases, which affects the emulsification effect of hexadecane.

[0100] Comparative Example 3

[0101] Compared with Example 1, the proportion of maleic anhydride is 0.05 parts, the content of the auxiliary agent is too low, the degree of cross-linking modification of the fungal protein is low, and the hydrophobic region of the protein molecule is not fully exposed, which affects the contact emulsification effect with the oil phase hexadecane.

[0102] Comparative Example 4

[0103] Compared with Example 1, the proportion of maleic anhydride is 1.5 parts, the content of the auxiliary agent is too high, and the degree of cross-linking modification of the fungal protein is too large, resulting in denaturation of the protein molecules and a significant reduction in the amphiphilicity of the molecules.

[0104] Comparative Example 5

[0105] Compared with Example 1, the amount of polyethylene oxide is 0.05 parts, the thickener content is insufficient, the emulsion is separated, and the oil and water are separated.

[0106] Comparative Example 6

[0107] Compared with Example 1, the amount of polyethylene oxide is 1.5 parts, the thickener content is too high, the emulsion is thick and has poor fluidity.

[0108] Comparative Example 7

[0109] Compared with Example 1, the pH is 3. The pH of the solution environment is far away from the isoelectric point of the fungal protein, and excessive acidity causes the protein molecule structure to be unstable, and the amphiphilic properties of the protein molecule are greatly reduced.

[0110] Comparative Example 8

[0111] Compared with Example 1, the pH is 9. The pH of the solution environment is far away from the isoelectric point of the fungal protein, and excessive alkalinity leads to unstable protein molecular structure and greatly reduced amphiphilic properties of the protein molecule.

[0112] Comparative Example 9

[0113] Compared with Example 1, the ultrasonic temperature is controlled to be 0-5°C, which is lower than the melting point of hexadecane. The hexadecane is in a solid phase and has no emulsification effect.

[0114] Comparative Example 10

[0115] Compared with Example 3, the ultrasonic temperature is controlled at 30-50° C., which is higher than the boiling point of pentane. Pentane evaporates into gas phase and has no emulsification effect.

[0116] Comparative Example 11

[0117] Compared with Example 1, the emulsification effect of only using fungal protein to act on hexadecane is poor.

[0118] Table 1

[0119]

[0120] The above examples 1-10 all have good emulsification effects. Comparative Example 1 shows that when no fungal protein complex liquid is added and no biological surfactant is present, only magnetic stirring and vortex oscillation cannot form an amphoteric protein film at the oil-water interface, and hexadecane has no emulsification effect.

[0121] Table 2 OD600 is used to characterize turbidity

[0122]

[0123] Table 2 shows that in Examples 1-10, the turbidity values ​​measured by OD600 are high, indicating that the system is emulsified and turbid, characterized by good emulsification.

[0124] In Comparative Examples 1-11, the turbidity value measured by OD600 was close to 0, indicating that the system was not emulsified and turbid, characterized as not emulsified.

Claims

1. A fungal protein complex liquid of emulsified alkane, characterized in that: The composition is composed of the following components by mass: 0.5-2 parts of fungal protein, 2-5 parts of inorganic compounds, 2-5 parts of buffer salts, 2-5 parts of polyol compounds, 0.5-1 parts of thickeners, 0.5-1 parts of additives and 300-400 parts of deionized water; The inorganic compound is any one of sodium chloride and potassium chloride; The buffer salt is any one of phosphate, triethanolamine dodecyl sulfate, and sodium N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid; The polyol compound is any one of glycerol, polyethylene glycol, diethylene glycol, and sorbitol; The thickener is any one of polyethylene oxide, polyacrylamide, lecithin, sodium lauryl sulfate, monopalmitoyl glycerol, and dipalmitoyl glycerol; The auxiliary agent is any one of maleic anhydride, lysine, epoxy resin, 1,4-dioxane, and methacrylic acid; When using fungal protein complex liquid to emulsify alkane, the alkane, i.e., oil phase, is mixed with water phase, and the fungal protein complex liquid is added, wherein the volume ratio of the water phase to the oil phase is 1:1-55, and the mass ratio of the fungal protein complex liquid to the alkane is 1:0.5-2.

2. The method for preparing the fungal protein composite liquid according to claim 1, characterized in that The following steps are involved: First, fungal protein, inorganic compound, buffer salt, polyol compound and deionized water were mixed according to the following weight parts, and solution A was obtained after ultrasonic treatment: 0.5-2 parts of the fungal protein, 2-5 parts of the inorganic compound, 2-5 parts of the buffer salt, 2-5 parts of the polyol compound, and 100-150 parts of deionized water; Next, the thickener, additive and deionized water were mixed and filtered to obtain solution B: 0.5-1 part of the thickener, 0.5-1 part of the auxiliary agent and 200-250 parts of deionized water; Finally, solution A and solution B are mixed to obtain a fungal protein composite solution.

3. The use of the fungal protein composite liquid as a biosurfactant in emulsifying alkanes according to claim 1, characterized in that: The method comprises the following steps: adjusting the pH of the fungal protein complex liquid to 4-8, filtering and sterilizing the alkane, mixing the alkane, i.e. the oil phase, with the water phase, adding the fungal protein complex liquid, emulsifying by magnetic stirring and vortex oscillation, and finally degassing by ultrasonication; The volume ratio of the water phase to the oil phase is 1:1-55; The mass ratio of the fungal protein complex liquid to the alkane is 1:0.5-2; The alkane is a C5-C16 liquid alkane; The alkane filtration pore size range is 0.45-1 μm; The magnetic stirring conditions are 500-1000 rpm, 3-5 h, and the vortex oscillation time is 0.5-1 h; The ultrasonic temperature is controlled at 5-30°C, and the ultrasonic time is 12-48h.

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