Artificial islet body closed micro organ cultivation equipment

Abstract

The invention relates to artificial islet body closed micro organ cultivation equipment, and belongs to the field of micro organ cultivation, the artificial islet body closed micro organ cultivation equipment comprises a base, the base is fixedly connected with a support column, the upper part of the support column is fixedly connected with a support plate, the support plate is provided with a first cylinder, and the base is provided with a driving assembly; a second barrel is slidably mounted on the first barrel, a partition plate is fixedly connected to the upper portion of the second barrel, a third barrel is fixedly arranged on the partition plate, a switching assembly is mounted on the third barrel, a connecting block and a flow guide plate are mounted in the switching assembly, an opening is formed in the switching assembly, and the connecting block and the flow guide plate are arranged in the opening. And a first connecting cover is adhered to the supporting plate. The problems that an existing micro organ cultivation device cannot adjust the permeation effect in the device, existing equipment cannot be switched between adjustment of the pressure of a permeation structure in the device and supplementation of a culture solution, and the supporting mode cannot be changed according to the shape of a micro organ are solved.

Description

Technical Field

[0001] The invention relates to an artificial pancreatic islet closed micro-organ cultivation device, belonging to the field of micro-organ cultivation. Background Art

[0002] Diabetes is a serious chronic disease. Due to impaired or insufficient pancreatic β-cell function, patients suffer from insufficient or resistant insulin secretion, which in turn leads to hyperglycemia and its complications. Current treatments mainly rely on exogenous insulin injections or drug regulation, but these methods are inconvenient to use and difficult to control dosage. The cultivation of artificial pancreatic islet micro-organs provides a potential solution, but its safety and functional stability in the body remain challenges, and existing micro-organ cultivation equipment still has some shortcomings.

[0003] For example, the invention patent with publication number CN114774278B discloses a method for using a 3D heart valve organoid incubator, including a front incubation box and a back incubation box, the front incubation box includes a first box body and a first box cover, the first box cover is provided with a first liquid adding tank and a first culture tank that are interconnected, and the bottom of the first culture tank is provided with a microporous semipermeable membrane, and the back incubation box includes a second box body and a second box cover, the second box cover is provided with a second liquid adding tank and a second culture tank that are interconnected, and the bottom of the second culture tank is provided with a hollow support plate, and the first box body and the second box body are provided with a culture liquid cavity. During the incubation process, the endothelial cell layer and the interstitial cell layer can be kept fully nutrient-exchanged with the culture liquid, ensuring the integrity of the structure of the three-layer heart valve organoids to be cultivated, while avoiding cell apoptosis, greatly improving the success rate of cultivating heart valve organoids. Although the above-mentioned device can realize the function of nutrient exchange, it is unable to adjust the osmotic effect in the device when in use. The existing equipment is unable to switch between adjusting the osmotic structure pressure in the device and replenishing the culture solution, and is unable to adjust the placement angle of the micro-organs in the device in real time during the infiltration of the culture solution, and is unable to change the support method according to the shape of the micro-organs.

[0004] Therefore, we made improvements on this and proposed an artificial pancreatic islet closed micro-organ cultivation device. Summary of the invention

[0005] (I) The technical problem to be solved by the present invention is that the existing micro-organ cultivation device is unable to adjust the osmotic effect in the device, and is unable to adjust the placement angle of the micro-organ in the device in real time during the infiltration of the culture solution, and is unable to change the support method according to the shape of the micro-organ. The existing equipment is unable to switch between adjusting the osmotic structure pressure in the device and replenishing the culture solution.

[0006] (II) Technical solution In order to achieve the above-mentioned invention objectives, the present invention provides an artificial pancreatic islet closed micro-organ cultivation device, including a base, a pillar is fixedly connected to the base, a support plate is fixedly connected above the pillar, a first cylinder is installed on the support plate, a driving assembly is installed on the base, a second cylinder is slidably installed on the first cylinder, a partition is fixedly connected above the second cylinder, a third cylinder is fixedly arranged on the partition, a switching assembly is installed on the third cylinder, a connecting block and a guide plate are installed in the switching assembly, an opening is opened on the switching assembly, a first connecting cover is bonded to the support plate, a second connecting cover is rotatably connected to the outer side of the first connecting cover, a first microhole is opened on the first connecting cover, a second microhole is opened on the second connecting cover, and an adjustment assembly is installed on the second connecting cover.

[0007] Among them, the driving assembly includes a motor fixedly mounted on the base, a gear is connected to the output shaft of the motor, a gear is connected to the inner box of the gear, a connecting column is fixedly arranged on the gear ring, a connecting rod is fixedly connected to the connecting column, a supporting plate is fixedly arranged on the connecting rod, and the supporting plate and the supporting plate are rotatably connected.

[0008] Among them, a fixing cylinder is fixedly arranged in the support plate, a stud is threadedly installed in the fixing cylinder, a support block is fixedly arranged above the stud, the support block is made of rubber material, the interior of the support block is hollow, the top of the support block is a hemispherical cover structure, and the fixing cylinders are evenly distributed on the support plate.

[0009] Among them, the support plate is provided with an installation groove, a rubber connecting ring is installed in the installation groove, the rubber connecting ring and the first cylinder are fixedly connected, and the first cylinder forms a clamping structure with the support plate through the rubber connecting ring.

[0010] Among them, the switching component includes an inner cylinder slidably installed in the partition and the third cylinder, the inner cylinder and the third cylinder are fixedly provided with a fixed tube, the pipe mouth of the fixed tube close to the side of the central axis of the third cylinder is in contact with the inner cylinder, the first electric push rod and the sealing cylinder are fixedly provided on the partition, a connecting plate is slidably installed in the sealing cylinder, the connecting plate is connected to the top of the first electric push rod, the connecting plate and the inner cylinder are fixedly connected, and the partition, the third cylinder and the sealing cylinder are fixedly connected to form an integral structure.

[0011] Among them, the opening is opened on the right side of the inner tube, the inner tube, the connecting block and the guide plate are fixedly connected to form an integral structure, a hydrophobic layer is arranged on the guide plate, the guide plate is an arc-shaped plate, and the right side of the guide plate points to the opening.

[0012] Parts of the first connection cover having the first micropores and parts without the first micropores are distributed alternately, and parts of the second connection cover having the second micropores and parts without the second micropores are distributed alternately.

[0013] Among them, the adjustment assembly includes a first connecting rod fixedly connected to the second connecting cover, a first sealing plate fixedly connected to the first connecting rod, a second connecting rod fixedly connected to the first sealing plate, a third connecting rod fixedly arranged on the second connecting rod, a pointer installed on the top of the third connecting rod, a movable groove is opened in the first cylinder, and a second sealing plate is installed on the inner wall of the movable groove.

[0014] Among them, a docking groove is provided at the bottom of the second connecting rod, a groove is provided on the support plate, a rubber damping block is fixedly connected in the groove, the top of the rubber damping block protrudes from the upper surface of the support plate, and a scale mark is provided on the first cylinder, and the position of the scale mark corresponds to the position of the pointer tip.

[0015] Wherein, the artificial pancreatic islet body closed micro-organ cultivation device uses an artificial pancreatic islet body closed micro-organ cultivation method.

[0016] (III) Beneficial effects The artificial pancreatic islet closed micro-organ cultivation device provided by the present invention has the following beneficial effects: 1. By setting the adjustment component, the first connecting cover, the first micropore, the second connecting cover and the second micropore, in the initial state of the device, the positions of the first micropore and the second micropore on the first connecting cover and the second connecting cover are staggered with each other, and by pulling the third connecting rod, the second connecting cover is rotated on the first connecting cover, thereby changing the staggered state of the first micropore and the second micropore. When the overlapping area of ​​the first micropore and the second micropore is different, the penetration effect of the device will also change, which solves the problem that the existing micro-organ cultivation equipment cannot adjust the penetration effect in the device.

[0017] 2. Through the provided partition, third cylinder and switching assembly, when the first electric push rod on the switching assembly is extended, it will drive the inner cylinder to move upward. When the inner cylinder moves upward, the opening will be connected with the fixed tube, and the opening will be connected with the cavity between the partition and the third cylinder, so that the water flow can flow into the cavity between the partition and the third cylinder through the lower half of the opening again through the arc guide plate. At this time, the counterweight of the device can be increased to facilitate the subsequent adjustment of the osmotic pressure. The device can further extend the first electric push rod so that the opening is not connected with the cavity between the partition and the third cylinder. At this time, the opening is only connected with the second cylinder, and the culture fluid can be injected into the second cylinder. By changing the extension amount of the first electric push rod, the function of the device is changed, so that the device can switch between changing the osmotic pressure and supplementing the culture fluid.

[0018] 3. Through the drive assembly, the motor drives the gear to rotate, which drives the gear ring to rotate, thereby driving the connecting rod and the support plate to rotate. During the rotation of the support plate, the placement angle of the micro-organ in the device can be changed. At the same time, the height of the local support block can be changed by rotating the stud, so that the device can stably support micro-organs of different shapes, thereby enhancing the applicability of the device and solving the problem that the existing micro-organ cultivation equipment cannot change the support method according to the shape of the micro-organ. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the connection structure between the base and the drive assembly of the present invention; Figure 3 for Figure 2 A magnified schematic diagram of the structure at center A; Figure 4 This is a schematic diagram of the internal structure of the switching component of the present invention; Figure 5 for Figure 4 A magnified schematic diagram of the structure at B in the middle; Figure 6 for Figure 4 A magnified schematic diagram of the structure at C in the middle; Figure 7 for Figure 4 A magnified schematic diagram of the structure at D in the middle; Figure 8 for Figure 4 A magnified schematic diagram of the structure at E in the middle; Fig. 9 for Figure 4 A magnified schematic diagram of the structure at F in the middle; Fig.10 It is a schematic diagram of the fixing tube and stud structure of the present invention; Fig.11 It is a schematic diagram of the connection structure between the second connection cover and the adjustment assembly of the present invention; Fig.12 It is a schematic diagram of the split structure of the first connecting cover and the second connecting cover of the present invention.

[0021] Figure numerals: 1, base; 2, pillar; 3, driving assembly; 301, motor; 302, gear; 303, gear ring; 304, connecting column; 305, connecting rod; 306, supporting plate; 3061, fixing cylinder; 3062, stud; 3063, supporting block; 4, supporting plate; 5, first cylinder; 6, second cylinder; 7, partition; 8, third cylinder; 9, switching assembly; 901, inner cylinder; 902, fixing tube; 903, first electric push rod; 904, connecting plate; 905, sealing cylinder; 10 , connecting block; 11, opening; 12, guide plate; 13, hydrophobic layer; 14, rubber connecting ring; 15, mounting groove; 16, first connecting cover; 17, second connecting cover; 18, first micropore; 19, second micropore; 20, adjusting component; 2001, first connecting rod; 2002, first sealing plate; 2003, second connecting rod; 2004, third connecting rod; 2005, movable groove; 2006, second sealing plate; 2007, pointer; 21, groove; 22, rubber damping block; 23, docking groove. DETAILED DESCRIPTION

[0022] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples of the specification. The following examples are only used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0023] Embodiment 1: like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 , Fig. 9 , Fig.10 , Fig.11 and Fig.12As shown, this embodiment proposes an artificial pancreatic islet closed micro-organ cultivation device, including a base 1, a pillar 2 is fixedly connected to the base 1, a support plate 4 is fixedly connected above the pillar 2, a first cylinder 5 is installed on the support plate 4, a driving component 3 is installed on the base 1, the driving component 3 is used to support the micro-organ and change the angle of the micro-organ, so that the device can rotate the micro-organ while permeating the culture fluid, a second cylinder 6 is slidably installed on the first cylinder 5, and the slidably installed second cylinder 6 facilitates the subsequent increase in the osmotic pressure inside the device when the counterweight of the device is increased, a partition 7 is fixedly connected above the second cylinder 6, liquid can be injected above the partition 7, thereby increasing the counterweight of the partition 7, so as to subsequently increase the osmotic pressure, a third cylinder 8 is fixedly arranged on the partition 7, a switching component 9 is installed on the third cylinder 8, and a switching component 9 is installed inside the switching component 9 There are connecting blocks 10 and guide plates 12, and an opening 11 is provided on the switching component 9. When the opening 11 is connected to the outside of the device and the cavity between the third cylinder 8 and the partition 7, water can be injected into the device so that the water flows through the guide plate 12 into the lower half of the opening 11, and then the water flows into the cavity between the third cylinder 8 and the partition 7, thereby changing the counterweight of the device. The switching component 9 can also be used to allow the liquid in the cavity between the third cylinder 8 and the partition 7 to flow downward through the opening 11, thereby replenishing the culture fluid in the device. A first connecting cover 16 is bonded to the support plate 4, and a second connecting cover 17 is rotatably connected to the outer side of the first connecting cover 16. A first microhole 18 is provided on the first connecting cover 16, and a second microhole 19 is provided on the second connecting cover 17. An adjusting component 20 is installed on the second connecting cover 17. The adjustment component on the device is used to change the angle of the second connecting cover 17 on the first connecting cover 16, thereby changing the degree of interleaving of the first micropores 18 and the second micropores 19, so that the penetration effect of the penetration structure composed of the second connecting cover 17 and the first connecting cover 16 of the device changes.

[0024] Embodiment 2: The solution in Example 1 is further introduced below in combination with a specific working method, as described below: like Figure 1 , Figure 2 and Figure 3As shown, as a preferred embodiment, on the basis of the above-mentioned manner, further, the driving component 3 includes a motor 301 fixedly mounted on the base 1, a gear 302 is connected to the output shaft of the motor 301, a gear ring 303 is connected to the inner box of the gear 302, a connecting column 304 is fixedly provided on the gear ring 303, a connecting rod 305 is fixedly connected to the connecting column 304, a supporting disk 306 is fixedly provided on the connecting rod 305, and the supporting disk 306 and the supporting plate 4 are rotatably connected. The motor 301 on the device drives the gear 302 to rotate, driving the gear ring 303, the connecting column 304 and the connecting rod 305 to rotate, so that the supporting disk 306 rotates. The supporting disk 306 can change the angle of the support when rotating, thereby changing the placement angle of the micro-organ when the culture fluid is infiltrated.

[0025] like Figure 1 , Figure 2 , Figure 3 , Fig. 9 and Fig.10 As shown, as a preferred embodiment, on the basis of the above method, further, a fixing cylinder 3061 is fixedly provided in the support disk 306, a stud 3062 is installed on the inner thread of the fixing cylinder 3061, a support block 3063 is fixedly provided above the stud 3062, the material of the support block 3063 is rubber material, the interior of the support block 3063 is hollow, the top of the support block 3063 is a hemispherical cover structure, the fixing cylinder 3061 is evenly distributed on the support disk 306, the hollow support block 3063 inside can not only ensure the supporting effect, but also can reduce the pressure on the micro-organ, thereby improving the safety of the device when used, by rotating the stud 3062 in the fixing cylinder 3061, the stud 3062 drives the support block 3063 to move upward, thereby changing the supporting effect of the device.

[0026] like Figure 4 and Figure 6 As shown, as a preferred embodiment, on the basis of the above method, further, a mounting groove 15 is opened in the support plate 4, and a rubber connecting ring 14 is installed in the mounting groove 15. The rubber connecting ring 14 and the first cylinder 5 are fixedly connected. The first cylinder 5 and the support plate 4 form a clamping structure through the rubber connecting ring 14. The lower half of the rubber connecting ring 14 can be clamped and installed inside the mounting groove 15, so that the first cylinder 5 can maintain its own installation stability, and facilitate the subsequent disassembly of the first cylinder 5, thereby enhancing the convenience of the device when used.

[0027] like Figure 4 and Figure 7As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, the switching assembly 9 includes an inner cylinder 901 slidably mounted in the partition 7 and the third cylinder 8, the inner cylinder 901 and the third cylinder 8 are fixedly provided with a fixed tube 902, the tube opening of the fixed tube 902 close to the central axis of the third cylinder 8 and the inner cylinder 901 are fitted with each other, the partition 7 is fixedly provided with a first electric push rod 903 and a sealing cylinder 905, a connecting plate 904 is slidably mounted in the sealing cylinder 905, the connecting plate 904 is connected to the top of the first electric push rod 903, and the connecting plate 904 and the inner cylinder 901 are connected. For fixed connection, the partition 7, the third cylinder 8 and the sealing cylinder 905 are fixedly connected as an integral structure, and the inner cylinder 901 can slide in the partition 7 and the third cylinder 8. By extending or shortening the first electric push rod 903, the height of the inner cylinder 901 and the connecting plate 904 is changed, thereby changing the connection state of the inner cylinder 901. When the inner cylinder 901 is connected with the fixed tube 902 and the interior of the third cylinder 8, the liquid can be diverted to the interior of the third cylinder 8. When the inner cylinder 901 is only connected with the fixed tube 902, the culture fluid can be injected into the culture area of ​​the device, thereby achieving the effect of function switching.

[0028] like Figure 7 As shown, as a preferred embodiment, on the basis of the above method, further, the opening 11 is opened on the right side of the inner cylinder 901, the inner cylinder 901, the connecting block 10, and the guide plate 12 are fixedly connected as an integral structure, and a hydrophobic layer 13 is arranged on the guide plate 12, the guide plate 12 is an arc-shaped plate, and the right side of the guide plate 12 points to the opening 11, and the hydrophobic layer 13 on the guide plate 12 can prevent the liquid from being adsorbed on the surface of the guide plate 12, and the guide plate 12 with an arc structure can guide the liquid to the designated area, so as to adjust the overall weight of the device later, thereby changing the osmotic pressure.

[0029] like Fig.11 and Fig.12 As shown, as a preferred embodiment, on the basis of the above method, further, the part with the first micropores 18 on the first connecting cover 16 is alternately distributed with the part without the first micropores 18, and the part with the second micropores 19 on the second connecting cover 17 is alternately distributed with the part without the second micropores 19. The device can change the penetration effect of the device by rotating the second connecting cover 17 so that the overlapping area of ​​the first micropores 18 and the second micropores 19 changes.

[0030] like Figure 4 , Figure 5 and Fig.11As shown, as a preferred embodiment, on the basis of the above method, further, the adjusting component 20 includes a first connecting rod 2001 fixedly connected to the second connecting cover 17, a first sealing plate 2002 is fixedly connected to the first connecting rod 2001, a second connecting rod 2003 is fixedly connected to the first sealing plate 2002, a third connecting rod 2004 is fixedly arranged on the second connecting rod 2003, a pointer 2007 is installed on the top of the third connecting rod 2004, a movable groove 2005 is opened in the first cylinder 5, and a second sealing plate 2006 is installed on the inner wall of the movable groove 2005. The device can rotate the third connecting rod 2004 to rotate the first connecting rod 2001, the first sealing plate 2002 and the second connecting rod 2003, thereby adjusting the angle of the second connecting cover 17 for the subsequent penetration effect of the adjustment device, and the second sealing plate 2006 in the movable groove 2005 can ensure the overall sealing of the device.

[0031] like Figure 4 and Figure 5 As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, a docking groove 23 is provided at the bottom of the second connecting rod 2003, a groove 21 is provided on the support plate 4, a rubber damping block 22 is fixedly connected in the groove 21, the top of the rubber damping block 22 protrudes from the upper surface of the support plate 4, and a scale mark is provided on the first cylinder 5, the position of the scale mark corresponds to the position of the tip of the pointer 2007, and the protruding part of the rubber damping block 22 can provide resistance when the second connecting rod 2003 rotates, so that after the pointer 2007 reaches the appropriate position of the scale line, the second connecting rod 2003 can remain in the engaged state.

[0032] The artificial pancreatic islet body closed micro-organ cultivation device uses an artificial pancreatic islet body closed micro-organ cultivation method comprising: S1: Preparation of micro-organs: Pancreatic cells with insulin secretion function are cultivated through stem cell differentiation technology, and then cultured in a specific culture medium to form micro-organs.

[0033] S2: Design of the structure of the first connecting cover 16: Material selection: polylactic-co-glycolic acid (PLGA), polytetrafluoroethylene (PTFE) or titanium alloy, the surface can be modified for biocompatibility.

[0034] Pore ​​size: The pore size of the first micropore 18 and the second micropore 19 is controlled at 1-5 nanometers (for small molecule permeability) and 5-20 micrometers (for cell sealing).

[0035] Sealing of micro-organs: The micro-organs are placed in a designed first connection cover 16 to ensure that they do not come into contact with other cells in the body, but can secrete insulin through the pores.

[0036] S3: Implantation method: The sealed micro-organ is implanted into a specific location such as the abdomen through minimally invasive surgery to replace or assist the impaired pancreas.

[0037] Embodiment 3: The schemes in Example 1 and Example 2 are further introduced below in combination with specific working methods, as described below for details: Specifically, when the artificial pancreatic islet closed micro-organ cultivation device is used: Figure 1 , Figure 3 , Figure 4 , Figure 6 , Figure 7 , Fig. 9 and Fig.10 As shown, the base 1, the support 2 and the support plate 4 are used to support the device as a whole. The rubber connecting ring 14 is installed into the installation groove 15, and then the micro-organ is placed on the support plate 306. The first connecting cover 16 is bonded to the surface of the support plate 306. After the preparation work is completed, the second cylinder 6 is slidably installed in the first cylinder 5, and the culture liquid is injected through the fixed tube 902 on the third cylinder 8. The first electric push rod 903 on the switching component 9 is extended or shortened to change the height of the inner cylinder 901 and the connecting plate 904, thereby changing the connection state of the inner cylinder 901. When the inner cylinder 901 is connected with the fixed tube 902 and the inside of the third cylinder 8, the liquid can be diverted to the inside of the third cylinder 8. When the inner cylinder 901 is only connected with the fixed tube 902, the culture liquid can be injected into the culture area of ​​the device. Alternatively, the culture liquid can be injected into the cavity between the partition 7 and the third cylinder 8, and then the inner cylinder 901 is lowered so that the height of the bottom of the opening 11 is lower than the height of the liquid level in the cavity, thereby conveniently replenishing the culture liquid. The hydrophobic layer 13 on the guide plate 12 can prevent the liquid from being adsorbed on the surface of the guide plate 12, and the arc-shaped guide plate 12 can guide the liquid to the area on the right side of the opening 11 ( Figure 7 ), so as to subsequently adjust the overall weight of the device, thereby changing the osmotic pressure. During the process of the device infiltrating the culture fluid, the motor 301 can drive the gear 302 to rotate, driving the gear ring 303, the connecting column 304 and the connecting rod 305 to rotate, so that the support disk 306 rotates on the support plate 4. The support disk 306 can change the angle of the support when rotating, thereby changing the placement angle of the micro-organ when the culture fluid is infiltrated. By rotating the stud 3062 in the fixed cylinder 3061, the stud 3062 drives the support block 3063 to move upward, thereby changing the support effect of the device to adapt to the support of micro-tracheas of different shapes. The hollow support block 3063 inside can not only ensure the support effect, but also reduce the pressure on the micro-organ.

[0038] like Figure 1 , Figure 2 , Figure 4 , Figure 5 , Figure 8 , Fig.11 and Fig.12 As shown, the third connecting rod 2004 is rotated to make the first connecting rod 2001, the first sealing plate 2002 and the second connecting rod 2003 rotate in a circular motion, thereby adjusting the angle of the second connecting cover 17 on the first connecting cover 16 and changing the degree of staggering of the first micropores 18 and the second micropores 19 to facilitate the subsequent adjustment of the penetration effect of the device. The second sealing plate 2006 in the movable groove 2005 can be stably fitted with the first sealing plate 2002. Since the position of the scale mark corresponds to the position of the tip of the pointer 2007, after the pointer 2007 moves to the appropriate position of the scale line, the rubber damping block 22 of the protruding part can provide resistance when the second connecting rod 2003 rotates, so that the second connecting rod 2003 and the second connecting cover 17 can remain in a locked state after the adjustment is completed.

[0039] The above embodiments are only used to illustrate the present invention, but not to limit the present invention. Although the present invention is described in detail with reference to the embodiments, it should be understood by those skilled in the art that various combinations, modifications or equivalent substitutions of the technical solutions of the present invention do not depart from the spirit and scope of the technical solutions of the present invention, and should be included in the scope of the claims of the present invention.

Claims

1. An artificial pancreatic islet closed micro-organ cultivation device, comprising a base (1), characterized in that: The base (1) is fixedly connected to a pillar (2), a support plate (4) is fixedly connected above the pillar (2), a first cylinder (5) is mounted on the support plate (4), a driving assembly (3) is mounted on the base (1), a second cylinder (6) is slidably mounted on the first cylinder (5), a partition plate (7) is fixedly connected above the second cylinder (6), a third cylinder (8) is fixedly arranged on the partition plate (7), a switching assembly (9) is mounted on the third cylinder (8), a connecting block (10) and a guide plate (12) are mounted inside the switching assembly (9), an opening (11) is provided on the switching assembly (9), a first connecting cover (16) is bonded to the support plate (4), a second connecting cover (17) is rotatably connected to the outer side of the first connecting cover (16), a first micro hole (18) is provided on the first connecting cover (16), a second micro hole (19) is provided on the second connecting cover (17), and an adjusting assembly (20) is mounted on the second connecting cover (17).

2. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The driving assembly (3) comprises a motor (301) fixedly mounted on the base (1); a gear (302) is connected to the output shaft of the motor (301); a gear ring (303) is connected to the inner box of the gear (302); a connecting column (304) is fixedly arranged on the gear ring (303); a connecting rod (305) is fixedly connected to the connecting column (304); a supporting plate (306) is fixedly arranged on the connecting rod (305); and the supporting plate (306) and the supporting plate (4) are rotatably connected.

3. The artificial pancreatic islet closed micro-organ cultivation device according to claim 2, characterized in that: A fixing cylinder (3061) is fixedly arranged inside the support plate (306), a stud (3062) is installed in the internal thread of the fixing cylinder (3061), a supporting block (3063) is fixedly arranged above the stud (3062), the supporting block (3063) is made of rubber material, the interior of the supporting block (3063) is hollow, the top of the supporting block (3063) is a hemispherical cover structure, and the fixing cylinders (3061) are evenly distributed on the support plate (306).

4. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The support plate (4) is provided with a mounting groove (15), a rubber connecting ring (14) is installed in the mounting groove (15), the rubber connecting ring (14) and the first cylinder (5) are fixedly connected, and the first cylinder (5) forms a snap-fit ​​structure with the support plate (4) via the rubber connecting ring (14).

5. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The switching assembly (9) comprises an inner cylinder (901) slidably mounted in the partition (7) and the third cylinder (8); a fixed tube (902) is fixedly mounted on the inner cylinder (901) and the third cylinder (8); a tube opening of the fixed tube (902) close to the central axis of the third cylinder (8) and the inner cylinder (901) are in contact with each other; a first electric push rod (903) and a sealing cylinder (905) are fixedly mounted on the partition (7); a connecting plate (904) is slidably mounted in the sealing cylinder (905); the connecting plate (904) is connected to the top of the first electric push rod (903); the connecting plate (904) and the inner cylinder (901) are fixedly connected; the partition (7), the third cylinder (8) and the sealing cylinder (905) are fixedly connected to form an integral structure.

6. The artificial pancreatic islet closed micro-organ cultivation device according to claim 5, characterized in that: The opening (11) is opened on the right side of the inner cylinder (901); the inner cylinder (901), the connecting block (10) and the guide plate (12) are fixedly connected to form an integral structure; a hydrophobic layer (13) is provided on the guide plate (12); the guide plate (12) is an arc-shaped plate; and the right side of the guide plate (12) points to the opening (11).

7. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The portion of the first connection cover (16) having the first micropore (18) and the portion without the first micropore (18) are distributed alternately, and the portion of the second connection cover (17) having the second micropore (19) and the portion without the second micropore (19) are distributed alternately.

8. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The adjustment assembly (20) comprises a first connecting rod (2001) fixedly connected to a second connecting cover (17); a first sealing plate (2002) fixedly connected to the first connecting rod (2001); a second connecting rod (2003) fixedly connected to the first sealing plate (2002); a third connecting rod (2004) fixedly arranged on the second connecting rod (2003); a pointer (2007) installed on the top of the third connecting rod (2004); a movable groove (2005) is provided in the first cylinder (5); and a second sealing plate (2006) is installed on the inner wall of the movable groove (2005).

9. The artificial pancreatic islet closed micro-organ cultivation device according to claim 8, characterized in that: The bottom of the second connecting rod (2003) is provided with a docking groove (23), the support plate (4) is provided with a groove (21), a rubber damping block (22) is fixedly connected in the groove (21), the top of the rubber damping block (22) protrudes from the upper surface of the support plate (4), and the first cylinder (5) is provided with a scale mark, the position of the scale mark corresponds to the position of the tip of the pointer (2007).

10. The artificial pancreatic islet closed micro-organ cultivation device according to claim 1, characterized in that: The artificial pancreatic islet body closed micro-organ cultivation device uses an artificial pancreatic islet body closed micro-organ cultivation method.

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