Sampling device for lymphoma B cell receptor research and operation method

Abstract

The invention belongs to the technical field of medical sample sampling devices, and particularly relates to a sampling device for lymphoma B cell receptor research, the sampling device comprises a sampling cylinder and a control box, the control box is fixedly connected with one side of the sampling cylinder, the inner bottom of the sampling cylinder is fixedly connected with a semiconductor chilling plate and a temperature sensor, and a fixing plug is inserted into the top of the sampling cylinder in a threaded manner; the operation method comprises the following steps that S1, a control instruction is input through the touch screen, the controller controls the miniature negative pressure pump to be started, air in the sampling barrel is sucked out, and negative pressure is formed in the sampling barrel; s2, the blood sample enters the sampling cylinder, meanwhile, the temperature sensor monitors the temperature in the sampling cylinder and sends a monitoring signal to the controller, and the controller controls the semiconductor chilling plate to start for refrigeration. Through the arrangement of the temperature monitoring control mechanism, the temperature in the sampling cylinder can be effectively monitored and controlled, and sampled cells can be effectively stored, so that the use effect of the sampling device is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of medical sample sampling devices, and in particular to a sampling device for lymphoma B cell receptor research and an operating method. Background Art

[0002] B-cell lymphoma is a type of malignant tumor with strong heterogeneity. In the past, chemotherapy was the main treatment method. With the advent of the CD20 monoclonal antibody rituximab, the treatment of B-cell lymphoma has entered a new era. It has almost improved the cure rate of all B-cell lymphomas. However, some patients still have recurrence or metastasis after receiving standard immunochemotherapy. Therefore, relevant technical personnel still need to gradually study the proliferation, growth and drug resistance of B-cell lymphoma. During the experimental research process, sampling devices need to be used for sampling in advance.

[0003] However, the existing sampling device has a relatively simple structure and lacks a certain temperature monitoring and control mechanism, so the sampled cells cannot be effectively preserved, thereby affecting the use effect of the sampling device. Summary of the invention

[0004] 1. Technical problems to be solved The purpose of the present invention is to solve the problem that the sampling device in the prior art lacks a certain temperature monitoring and control mechanism and cannot effectively preserve the sampled cells, and to propose a sampling device and an operating method for lymphoma B cell receptor research.

[0005] 2. Technical solution In order to achieve the above object, the present invention adopts the following technical solutions: A sampling device for lymphoma B cell receptor research, comprising a sampling tube and a control box, wherein the control box is fixedly connected to one side of the sampling tube, a semiconductor refrigeration sheet and a temperature sensor are fixedly connected to the inner bottom of the sampling tube, a fixing plug is threadedly inserted at the top of the sampling tube, a sample inlet tube and an air outlet tube are fixedly inserted on the fixing plug, the top of the sample inlet tube is connected to a collection tube through a first hose, and a control valve is threadedly connected to the collection tube; The top of the control box is fixedly connected to a micro negative pressure pump, the micro negative pressure pump is connected to the air outlet pipe through a second hose, a touch screen is fixedly connected to one side of the control box, a controller, a memory, a communication module and a power supply are arranged in the control box, a mounting port is arranged on one side of the control box, a baffle is rotatably connected in the mounting port, a card block is fixedly connected to the top of the baffle, and a clamping mechanism corresponding to the card block is fixedly connected to the outer wall of the control box; The output ends of the power supply and temperature sensor are connected to the input end of the controller, the output end of the controller is respectively connected to the input ends of the semiconductor refrigeration plate, the memory and the micro negative pressure pump, and the output ends of the touch screen and the communication module are bidirectionally connected to the input end of the controller.

[0006] Preferably, a heat preservation cavity is arranged around the inner wall of the sampling tube, and heat preservation cotton is embedded in the heat preservation cavity.

[0007] Preferably, a placement cylinder corresponding to the collection tube is fixedly connected to the outer wall of the sampling cylinder.

[0008] Preferably, a handle is fixedly sleeved on the collection tube.

[0009] Preferably, a transparent window is provided in the middle of the fixing plug.

[0010] Preferably, a charging connector is fixedly embedded on the outer wall of the control box, and the output end of the charging connector is connected to the input end of the power supply.

[0011] Preferably, the clamping mechanism includes a fixed block, a clamping rod is slidably inserted on the fixed block, a clamping slot corresponding to the clamping rod is provided on the clamping block, a clamping sleeve is fixedly connected to one end of the clamping rod close to the clamping block, and a pull rod is fixedly connected to one end of the clamping rod away from the clamping sleeve.

[0012] Preferably, a spring is sleeved on the clamping rod, and two ends of the spring are fixedly connected to the clamping sleeve and the fixing block respectively.

[0013] The present invention also proposes an operation method of a sampling device for lymphoma B cell receptor research, comprising the following steps: S1: First, insert the collection tube into the blood sample to be tested, and then input the control command through the touch screen. The controller controls the micro negative pressure pump to start and suck out the air in the sampling tube to form a negative pressure inside. S2: Then the blood sample enters the sampling tube through the collection tube, the first hose and the injection tube. At the same time, the temperature sensor monitors the temperature in the sampling tube and sends the monitoring signal to the controller. The controller controls the semiconductor refrigeration piece to start cooling.

[0014] 3. Beneficial effects Compared with the prior art, the advantages of the present invention are: (1) In the present invention, by setting up the temperature monitoring and control mechanism, the temperature in the sampling tube can be effectively monitored and controlled, and the sampled cells can be effectively preserved, thereby ensuring the use effect of the sampling device.

[0015] (2) In the present invention, the setting of the handle can facilitate the operation of the collection tube, the setting of the placement tube can place the collection tube, and the setting of the transparent window can view the situation in the sampling tube. At the same time, the setting of the heat preservation chamber and the heat preservation cotton can play a role in heat preservation.

[0016] (3) In the present invention, the charging connector can conveniently charge the power supply, and the clamping mechanism can conveniently fix the baffle. The setting of the communication module can receive and send remote control instructions. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of a sampling device for lymphoma B cell receptor research proposed by the present invention; Figure 2 This is a schematic diagram of the top view of the fixed plug of a sampling device for lymphoma B cell receptor research proposed by the present invention; Figure 3 for Figure 1 A schematic diagram of the structure at A; Figure 4 This is a schematic diagram of a control system of a sampling device for lymphoma B cell receptor research proposed by the present invention.

[0018] In the figure: 1 sampling tube, 2 control box, 3 semiconductor refrigeration plate, 4 temperature sensor, 5 fixing plug, 6 inlet tube, 7 outlet pipe, 8 first hose, 9 collection tube, 10 control valve, 11 micro negative pressure pump, 12 second hose, 13 touch screen, 14 controller, 15 memory, 16 communication module, 17 power supply, 18 baffle, 19 card block, 20 insulation cotton, 21 placement tube, 22 handle, 23 transparent window, 24 charging connector, 25 fixing block, 26 clamp rod, 27 clamp sleeve, 28 pull rod, 29 spring. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0020] Embodiment 1: Reference Figure 1-4 A sampling device for lymphoma B cell receptor research, comprising a sampling tube 1 and a control box 2, a heat preservation cavity is arranged around the inner wall of the sampling tube 1 to play a heat preservation role, a heat preservation cotton 20 is embedded in the heat preservation cavity, the control box 2 is fixedly connected to one side of the sampling tube 1, and a semiconductor refrigeration sheet 3 and a temperature sensor 4 are fixedly connected to the inner bottom of the sampling tube 1 to cool and monitor the temperature in the sampling tube 1; In the present invention, a fixed plug 5 is threadedly inserted at the top of the sampling tube 1, and a transparent window 23 is provided in the middle of the fixed plug 5 for viewing the situation in the sampling tube 1. A sample inlet tube 6 and an air outlet tube 7 are fixedly inserted on the fixed plug 5. The top of the sample inlet tube 6 is connected to a collection tube 9 through a first hose 8 for collecting blood samples. A placement tube 21 corresponding to the collection tube 9 is fixedly connected to the outer wall of the sampling tube 1 for convenient placement of the collection tube 9. A control valve 10 is threadedly connected to the collection tube 9, and a handle 22 is fixedly sleeved on the collection tube 9 for convenient movement of the collection tube 9. In the present invention, a micro negative pressure pump 11 is fixedly connected to the top of the control box 2, which is used to form a negative pressure inside the sampling tube 1. The micro negative pressure pump 11 is connected to the air outlet pipe 7 through a second hose 12. A touch screen 13 is fixedly connected to one side of the control box 2. A controller 14, a memory 15, a communication module 16 and a power supply 17 are arranged in the control box 2. A mounting port is arranged on one side of the control box 2. A baffle 18 is rotatably connected in the mounting port to protect the control element. A clamping block 19 is fixedly connected to the top of the baffle 18. A clamping mechanism corresponding to the clamping block 19 is fixedly connected to the outer wall of the control box 2 to fix the baffle 18. In the present invention, the clamping mechanism includes a fixed block 25, on which a clamping rod 26 is slidably inserted, and a clamping groove corresponding to the clamping rod 26 is provided on the clamping block 19, and a clamping sleeve 27 is fixedly sleeved on one end of the clamping rod 26 close to the clamping block 19, and a pull rod 28 is fixedly connected to one end of the clamping rod 26 away from the clamping sleeve 27, so as to facilitate pulling the clamping rod 26, and a spring 29 is sleeved on the clamping rod 26, and the two ends of the spring 29 are respectively fixedly connected to the clamping sleeve 27 and the fixed block 25, so as to provide a certain elastic support for the clamping rod 26; In the present invention, the output ends of the power supply 17 and the temperature sensor 4 are connected to the input end of the controller 14, and the output end of the controller 14 is respectively connected to the input ends of the semiconductor refrigeration film 3, the memory 15 and the micro negative pressure pump 11, so as to control the semiconductor refrigeration film 3 and the micro negative pressure pump 11. The output ends of the touch screen 13 and the communication module 16 are bidirectionally connected to the input end of the controller 14. A charging connector 24 is fixedly embedded on the outer wall of the control box 2, and the output end of the charging connector 24 is connected to the input end of the power supply 17.

[0021] In the present invention, a method for operating a sampling device for studying lymphoma B cell receptors comprises the following steps: S1: First, insert the collection tube 9 into the blood sample to be tested, then input the control command through the touch screen 13, and the controller 14 controls the micro negative pressure pump 11 to start, suck out the air in the sampling tube 1, and form a negative pressure inside it; S2: Then the blood sample enters the sampling tube 1 through the collection tube 9, the first hose 8 and the injection tube 6. At the same time, the temperature sensor 4 monitors the temperature in the sampling tube 1 and sends the monitoring signal to the controller 14. The controller 14 controls the semiconductor refrigeration plate 3 to start cooling.

[0022] In the present invention, by setting up the temperature monitoring and control mechanism, the temperature in the sampling tube can be effectively monitored and controlled, and the sampled cells can be effectively preserved, thereby ensuring the use effect of the sampling device.

[0023] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A sampling device for lymphoma B cell receptor research, comprising a sampling tube (1) and a control box (2), characterized in that: The control box (2) is fixedly connected to one side of the sampling cylinder (1); a semiconductor cooling plate (3) and a temperature sensor (4) are fixedly connected to the inner bottom of the sampling cylinder (1); a fixing plug (5) is threadedly inserted at the top of the sampling cylinder (1); a sample injection tube (6) and an air outlet tube (7) are fixedly inserted on the fixing plug (5); the top of the sample injection tube (6) is connected to a collection tube (9) via a first hose (8); and a control valve (10) is threadedly connected to the collection tube (9); A micro negative pressure pump (11) is fixedly connected to the top of the control box (2), and the micro negative pressure pump (11) is communicated with the air outlet pipe (7) via a second hose (12). A touch screen (13) is fixedly connected to one side of the control box (2). A controller (14), a memory (15), a communication module (16) and a power supply (17) are provided in the control box (2). A mounting opening is provided on one side of the control box (2), and a baffle (18) is rotatably connected to the mounting opening. A clamping block (19) is fixedly connected to the top of the baffle (18), and a clamping mechanism corresponding to the clamping block (19) is fixedly connected to the outer wall of the control box (2); The output ends of the power supply (17) and the temperature sensor (4) are both connected to the input end of the controller (14); the output end of the controller (14) is respectively connected to the input ends of the semiconductor cooling sheet (3), the memory (15) and the micro negative pressure pump (11); and the output ends of the touch screen (13) and the communication module (16) are both bidirectionally connected to the input end of the controller (14).

2. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: A heat preservation cavity is arranged around the inner wall of the sampling tube (1), and heat preservation cotton (20) is embedded in the heat preservation cavity.

3. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: A placement cylinder (21) corresponding to the collection tube (9) is fixedly connected to the outer wall of the sampling cylinder (1).

4. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: A handle (22) is fixedly sleeved on the collection tube (9).

5. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: A transparent window (23) is provided in the middle of the fixing plug (5).

6. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: A charging connector (24) is fixedly embedded on the outer wall of the control box (2), and an output end of the charging connector (24) is connected to an input end of a power source (17).

7. A sampling device for lymphoma B cell receptor research according to claim 1, characterized in that: The clamping mechanism comprises a fixed block (25), a clamping rod (26) is slidably inserted on the fixed block (25), a clamping groove corresponding to the clamping rod (26) is provided on the clamping block (19), a clamping sleeve (27) is fixedly sleeved on one end of the clamping rod (26) close to the clamping block (19), and a pull rod (28) is fixedly connected to one end of the clamping rod (26) away from the clamping sleeve (27).

8. A sampling device for lymphoma B cell receptor research according to claim 7, characterized in that: A spring (29) is sleeved on the clamping rod (26), and two ends of the spring (29) are respectively fixedly connected to the clamping sleeve (27) and the fixing block (25).

9. The method for operating the sampling device for lymphoma B cell receptor research according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1: first insert the collection tube (9) into the blood sample to be tested, then input a control command through the touch screen (13), and the controller (14) controls the micro negative pressure pump (11) to start, so as to suck out the air in the sampling tube (1) and form a negative pressure inside the sampling tube; S2: The blood sample then enters the sampling tube (1) through the collection tube (9), the first hose (8) and the sample injection tube (6). At the same time, the temperature sensor (4) monitors the temperature in the sampling tube (1) and sends a monitoring signal to the controller (14). The controller (14) controls the semiconductor refrigeration sheet (3) to start cooling.

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