Method for removing odor in breeding of hermetia illucens by pretreating plant base material through liquid fermentation

Abstract

The invention relates to the field of biotechnology, and particularly discloses a method for removing odor in breeding of hermetia illucens by pretreating a plant base material through liquid fermentation. A method for removing odor in breeding of hermetia illucens by pretreating a plant base material through liquid fermentation comprises the following steps: preparing the base material: uniformly stirring and mixing the plant base material for later use; preparing fermentation water: adding water and bacterial powder containing lactic acid bacteria and saccharomycetes into the plant-based material, and fermenting until the pH value of the mixed solution is 4.0-5.2 to obtain the fermentation water; and feeding: adding water into the fermentation water for dilution to obtain diluted fermentation water, then adding the diluted fermentation water into the plant-based material until the water content of the material is 60-75% to obtain a feed, and feeding the hermetia illucens with the feed.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a method for removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation. Background Art

[0002] Black soldier flies are saprophytic insects of the family Solenopsis that like to eat rotten food. Such materials often produce a strong rancid smell. In addition, a large amount of ammonia odor is produced during the breeding process of black soldier flies, part of which is caused by the decomposition of microorganisms, and the other part is emitted by the growth and development metabolism of the black soldier flies themselves, which is crucial to the growth and development of black soldier fly larvae. The large amount of ammonia emissions in black soldier fly breeding has always been a problem that has troubled the industry and academia.

[0003] Therefore, black soldier flies are often farmed in remote areas far away from residential areas and water sources. However, due to changes in wind direction and other reasons, residents will still be affected. Recently, related complaints have occurred from time to time. At present, industrial production often uses kitchen waste for breeding. The ammonia produced during the breeding process is irritating and difficult to breathe, and the working environment of workers is very poor. At present, the main measures used to address this problem are to use exhaust fans to increase the exhaust volume of the breeding shed; or to use deodorants used in livestock and poultry breeding, and install a large number of spray heads inside the farm. After the deodorant liquid is atomized into tiny particles through the spray heads, it is evenly diffused throughout the breeding environment. On the one hand, these methods require increased equipment investment, and on the other hand, the deodorization effect is poor and not long-lasting, and some deodorants even affect the growth of black soldier flies. Summary of the invention

[0004] In order to reduce the odor problem in the black soldier fly breeding process, the present application provides a method for removing the odor in the black soldier fly breeding process by pre-treating plant substrates through liquid fermentation.

[0005] The present application provides a method for removing odor in black soldier fly breeding by pre-treating plant substrates through liquid fermentation, which adopts the following technical scheme: A method for removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation comprises the following steps: Prepare base material: stir and mix the plant-based materials evenly and set aside; Preparation of fermentation water: adding water and bacterial powder containing lactic acid bacteria and yeast to the plant-based material, and fermenting the mixture so that the pH value of the mixture is 4.0-5.2 to obtain fermentation water; Feeding: adding water to the fermented water for dilution to obtain diluted fermented water, and then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75%, to obtain feed, and using the feed to feed the black soldier flies.

[0006] By adopting the above technical scheme, black soldier flies are raised with plant-based materials, which produces less odor, and fermented water is used for feeding materials. The black soldier flies are harvested after 10-15 days of breeding. During this period, the material temperature rises, which just provides a good temperature for microbial reproduction. The black soldier flies wriggle to eat the material, which can fluff the material and allow oxygen to pass through. The low pH of the fermented water and the continuous fermentation of the material to produce acid, enzymes and other aromatic substances can reduce the odor problem in black soldier fly breeding. In addition, while reducing the odor problem, it was unexpectedly found that the growth of black soldier flies can also be promoted.

[0007] In a specific implementation scheme, the step of preparing the base material is: stirring and mixing the plant-based material and food waste evenly to obtain a mixed material for later use.

[0008] By adopting the above technical solution and adding food waste to plant-based materials, the growth of black soldier flies can be further promoted.

[0009] In a specific embodiment, the plant-based material includes a mixture of palm meal and cassava residue, and the weight ratio of the palm meal to the cassava residue is 2:3 or 1:4.

[0010] By adopting the above technical solution, a composite plant-based material composed of palm meal and cassava residue is selected, and the ratio of palm meal and cassava residue is limited, so that the growth of black soldier flies is guaranteed while the generation of odor is reduced.

[0011] In a specific embodiment, the fermentation water preparation step is: adding water, bacterial powder containing lactic acid bacteria and yeast, and sugar to the plant-based material, fermenting until the pH of the mixed solution is 4.0-5.2, and obtaining fermentation water.

[0012] By adopting the above technical solution, the addition of sugar can provide energy and nutrition for microorganisms and improve the fermentation effect of fermentation water.

[0013] In a specific embodiment, in the fermentation water preparation step, the weight ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based materials, and sugar is 500: (0.5-1): (25-100): (5-50).

[0014] In a specific embodiment, in the fermentation water preparation step, the fermentation temperature is 24-40° C., and the fermentation time is 24-120 h.

[0015] By adopting the above technical scheme, the ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based materials, sugar, and the fermentation temperature and time are further limited, thereby further improving the fermentation effect of the fermented water.

[0016] In a specific embodiment, in the feeding step, when water is added to the fermentation water for dilution, the weight ratio of the fermentation water to water is 1:(3-5).

[0017] In a specific embodiment, the step of preparing the base material is: stirring and mixing the plant-based material evenly, and then performing solid-state fermentation for later use.

[0018] By adopting the above technical solution and first subjecting the plant-based materials to solid-state fermentation, the odor problem in the black soldier fly breeding process can be further reduced.

[0019] In a specific feasible implementation method, the feeding step is as follows: adding water to the fermentation water for dilution to obtain diluted fermentation water, and then adding the diluted fermentation water to the plant-based material so that the moisture content of the material is 60-75%, thereby obtaining feed, and using the feed to feed the black soldier flies; adding 40%-80% of the plant-based material 2-3 days before collecting the black soldier flies, and adjusting the moisture content of the material to 60%-75% with diluted fermentation water; after collecting the insects, adjusting the moisture content of the feed waste to 35%-60% with diluted fermentation water, and then directly loading it into a fermentation bag, and fermenting it at 20-40°C for 2-15 days, so that the material has a wine aroma, no odor, and a pH value of ≤4.8, and the fermentation is terminated to obtain livestock and poultry breeding products.

[0020] By adopting the above technical solution, livestock and poultry breeding products are produced using feed waste, thereby improving the utilization rate of materials.

[0021] In a specific embodiment, the feeding step is: adding water to the fermentation water to dilute it to obtain diluted fermentation water, then adding the diluted fermentation water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed the black soldier flies; after collecting the insects, stirring and mixing the insect sand, plant-based material, and sugar to obtain a mixture, then adding the diluted fermentation water to the mixture so that the moisture content of the mixture is 35%-60%, putting it into a fermentation bag, sealing and fermenting it at 20-40°C for 2-15 days so that the pH of the mixture is ≤4.8 and has a strong wine aroma, drying and crushing to obtain crushed material A; drying and crushing the insects to obtain crushed material B, stirring and mixing crushed material B with crushed material A to obtain a new fermentation-per-abdominal pre-digested protein product; wherein the weight ratio of insect sand, plant-based material, and sugar in the mixture is (30-70): (30-70): (1-5).

[0022] By adopting the above technical scheme, using insects, insect sand, plant-based materials, sugar, and diluted fermentation water, a high-protein new type of fermentation-per-abdominal pre-digested protein product can be prepared, thereby improving the utilization rate of materials.

[0023] In summary, the present application includes at least one of the following beneficial technical effects: 1. In this application, plant-based materials are used to feed black soldier flies, which produces less odor, and fermented water is used for feeding materials. The black soldier flies are harvested after 10-15 days of feeding. During this period, the material temperature rises, which just increases the good temperature for microbial reproduction. The black soldier flies crawl to eat the material, which can fluff the material and circulate oxygen. The low pH of the fermented water and the continuous fermentation of the material to produce acid, enzymes and other aromatic substances can reduce the odor problem of black soldier fly breeding. In addition, while reducing the odor problem, it was unexpectedly found that the growth of black soldier flies can also be promoted. 2. The addition of sugar in this application can provide energy and nutrition for microorganisms and improve the fermentation effect of fermentation water; 3. In this application, insects, insect sand, plant-based materials, sugar, and diluted fermentation water are used to produce a high-protein new fermentation-pre-digested protein product, thereby improving the utilization rate of materials. DETAILED DESCRIPTION

[0024] The present application is further described in detail below with reference to the embodiments.

[0025] All raw materials in the embodiment can be obtained commercially. The bacterial powder containing lactic acid bacteria and yeast is a composite microbial preparation product provided by Biosource Biotechnology (Shenzhen) Co., Ltd., with the trade name of Sufa 88; plant-based materials include but are not limited to a mixture of one or more of palm dregs, coconut dregs, cassava residues, mulberry leaves, elephant leaves, bran, silkworm litter, fungus residues, wheat flour, DDGS, distiller's grains, and bean dregs, preferably a mixture of palm dregs and cassava residues in this application; sugar includes but is not limited to a mixture of one or more of brown sugar, molasses, and glucose, preferably molasses in this application; QF-deodorizing working fluid is provided by Shanghai Qifit Environmental Protection Biotechnology Co., Ltd. Example

[0026] Example 1 Example 1 provides a method for removing odor in black soldier fly breeding by pre-treating plant substrates through liquid fermentation, comprising the following steps: Prepare base material: stir and mix the plant-based material evenly for use; the plant-based material is a mixture of palm dregs and cassava residues, and the weight ratio of palm dregs to cassava residues is 2:3; Preparation of fermentation water: adding water and bacterial powder containing lactic acid bacteria and yeast to part of the plant-based materials, fermenting at 24°C for 120 hours, so that the pH of the mixed solution is 4.0-5.2, to obtain fermentation water; wherein the weight ratio of water, bacterial powder containing lactic acid bacteria and yeast, and plant-based materials is 500:0.5:25; Feeding: adding water to the fermented water for dilution to obtain diluted fermented water, wherein the weight ratio of the fermented water to the water is 1:4, then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed the black soldier flies aged 4-6 days. During the feeding process, if the moisture content of the feed is lower than 60%, continue to add the diluted fermented water so that the moisture content of the feed is not lower than 60%. The specific feeding method is: adding 15 kg of plant-based material and 45 L of diluted fermented water on the first day; adding 10 L of diluted fermented water and turning the feed on the fourth day; adding 15 kg of plant-based material and 60 L of diluted fermented water on the fifth day and turning the feed; turning the feed on the sixth day; adding 20 L of diluted fermented water and turning the feed on the seventh day; adding 10 L of diluted fermented water and turning the feed on the eighth day; adding 20 kg of plant-based material and 60 L of diluted fermented water and turning the feed on the ninth day; turning the feed on the tenth day; adding 30 kg of plant-based material and 90 L of water on the eleventh day; and collecting the insects on the fourteenth day.

[0027] Example 2 Example 2 provides a method for removing odor in black soldier fly breeding by pre-treating plant substrates through liquid fermentation, comprising the following steps: Prepare base material: stir and mix the plant-based material evenly for use; the plant-based material is a mixture of palm dregs and cassava residues, and the weight ratio of palm dregs to cassava residues is 2:3; Preparation of fermentation water: adding water, powder containing lactic acid bacteria and yeast, and sugar to part of the plant-based materials, and fermenting at 24° C. for 120 hours, so that the pH of the mixed solution is 4.0-5.2, to obtain fermentation water; wherein the weight ratio of water, powder containing lactic acid bacteria and yeast, plant-based materials, and sugar is 500:0.5:25:5; wherein the sugar is molasses; Feeding: adding water to the fermented water for dilution to obtain diluted fermented water, wherein the weight ratio of the fermented water to the water is 1:4, then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed the black soldier flies aged 4-6 days. During the feeding process, if the moisture content of the feed is lower than 60%, continue to add the diluted fermented water so that the moisture content of the feed is not lower than 60%. The specific feeding method is: adding 15 kg of plant-based material and 45 L of diluted fermented water on the first day; adding 10 L of diluted fermented water and turning the feed on the fourth day; adding 15 kg of plant-based material and 60 L of diluted fermented water on the fifth day and turning the feed; turning the feed on the sixth day; adding 20 L of diluted fermented water and turning the feed on the seventh day; adding 10 L of diluted fermented water and turning the feed on the eighth day; adding 20 kg of plant-based material and 60 L of diluted fermented water and turning the feed on the ninth day; turning the feed on the tenth day; adding 30 kg of plant-based material and 90 L of water on the eleventh day; and collecting the insects on the fourteenth day.

[0028] Example 3 The difference between Example 3 and Example 2 is that, in the preparation of fermentation water, water, powder containing lactic acid bacteria and yeast, and sugar are added to part of the plant-based material, and fermentation is carried out at 24°C for 120 hours so that the pH of the mixed solution is 4.0-5.2 to obtain fermentation water; wherein the weight ratio of water, powder containing lactic acid bacteria and yeast, plant-based material, and sugar is 500:0.8:62:27; the remaining steps are consistent with Example 2.

[0029] Example 4 The difference between Example 4 and Example 2 is that fermentation water is prepared by adding water, bacterial powder containing lactic acid bacteria and yeast, and sugar to part of the plant-based material, and fermenting at 24°C for 120 hours so that the pH of the mixed solution is 4.0-5.2 to obtain fermentation water; wherein the weight ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based material, and sugar is 500:1:100:50; and the remaining steps are consistent with Example 2.

[0030] Example 5 The difference between Example 5 and Example 3 is that fermentation water is prepared by adding water, bacterial powder containing lactic acid bacteria and yeast, and sugar to part of the plant-based material, and fermenting at 32°C for 72 hours to make the pH of the mixed solution = 4.0-5.2 to obtain fermentation water; wherein the weight ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based material, and sugar is 500:0.8:62:27; the remaining steps are consistent with Example 3.

[0031] Example 6 The difference between Example 6 and Example 3 is that, in the preparation of fermentation water, water, powder containing lactic acid bacteria and yeast, and sugar are added to part of the plant-based material, and fermentation is carried out at 40°C for 24 hours so that the pH of the mixed solution is 4.0-5.2 to obtain fermentation water; wherein the weight ratio of water, powder containing lactic acid bacteria and yeast, plant-based material, and sugar is 500:0.8:62:27; and the remaining steps are consistent with Example 3.

[0032] Example 7 The difference between Example 7 and Example 5 is that, in preparing the base material: the plant-based material is stirred and mixed evenly for use; wherein the plant-based material is a mixture of palm meal and cassava residue, and the weight ratio of palm meal to cassava residue is 1:4; the remaining steps are consistent with Example 5.

[0033] Example 8 The difference between Example 8 and Example 7 is that, in preparing the base material, the plant-based material is stirred and mixed evenly, and then solid-state fermented for use; wherein the plant-based material is a mixture of palm meal and cassava residue, and the weight ratio of palm meal to cassava residue is 1:4; the remaining steps are consistent with Example 7.

[0034] Example 9 The difference between Example 9 and Example 1 is that, in preparing the base material, the plant-based material and the food waste are stirred and mixed evenly, and then solid-state fermentation is carried out for use; wherein the plant-based material is a mixture of palm meal and cassava residue, and the weight ratio of palm meal to cassava residue is 1:4; the weight ratio of the plant-based material to the food waste is 9:1; and the remaining steps are consistent with Example 1.

[0035] Comparative Example Comparative Example 1 Comparative Example 1 is a QF-deodorizing working liquid. During the black soldier fly breeding process, the QF-deodorizing working liquid is used for deodorization according to the deodorizing method of the QF-deodorizing working liquid.

[0036] Comparative Example 2 Comparative Example 2 provides a method for removing odor in black soldier fly breeding using a plant-based material, comprising the following steps: Prepare base material: stir and mix the plant-based material evenly for use; the plant-based material is a mixture of palm dregs and cassava residues, and the weight ratio of palm dregs to cassava residues is 2:3; Feeding: adding water to part of the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed black soldier flies aged 4-6 days. During the feeding process, if the moisture content of the feed is lower than 60%, continue to add water so that the moisture content of the feed is not lower than 60%. The specific feeding method is: adding 15 kg of plant-based material and 45 L of water on the first day; adding 10 L of water and turning the feed on the fourth day; adding 15 kg of plant-based material and 60 L of water and turning the feed on the fifth day; turning the feed on the sixth day; adding 20 L of water and turning the feed on the seventh day; adding 10 L of water and turning the feed on the eighth day; adding 20 kg of plant-based material and 60 L of water and turning the feed on the ninth day; turning the feed on the tenth day; adding 30 kg of plant-based material and 90 L of water on the eleventh day; and collecting the insects on the fourteenth day.

[0037] Application Examples Application Example 1 Application Example 1 provides a method for preparing livestock and poultry breeding products, comprising the following steps: Feeding: adding water to the fermented water in Example 1 to dilute it to obtain diluted fermented water, wherein the weight ratio of fermented water to water is 1:4, and then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75%, to obtain feed, and using the feed to feed black soldier flies aged 4-6 days. During the feeding process, if the moisture content in the feed is less than 60%, continue to add diluted fermented water so that the moisture content in the feed is not less than 60%. The specific feeding method is: on the first day, add 15 kg of plant-based material and 45 L of diluted fermented water; on the fourth day, add 10 L of diluted fermented water and turn the feed; on the fifth day, add 15 kg of plant-based material and 60 L of diluted fermented water and turn the feed; on the sixth day, turn the feed; on the seventh day, add diluted fermented water. 20L of fermentation water is added and the feed is turned over; on the 8th day, 10L of diluted fermentation water is added and the feed is turned over; on the 9th day, 20kg of plant-based materials are added and 60L of diluted fermentation water is added and the feed is turned over; on the 10th day, the feed is turned over; on the 11th day, 30kg of plant-based materials and 90L of water are added; on the 14th day, the insects are collected; on the 12th day, additional plant-based materials accounting for 60% of the total dry weight percentage of the fed feed are added, and the water content of the materials is adjusted to 60% with diluted fermentation water; after collecting the insects, the water content of the feed waste is adjusted to 47% with diluted fermentation water, and then directly loaded into the fermentation bag, and the feed waste is directly loaded into the fermentation bag, and fermented at 30°C for 8 days, so that the material has a wine aroma and no odor, and the pH is ≤4.8, and the fermentation is completed to obtain livestock and poultry breeding products.

[0038] Application Example 2 Application Example 2 provides a novel method for preparing a fermentation-percutaneous pre-digestion protein product, comprising the following steps: Feeding: adding water to the fermented water in Example 1 to dilute it to obtain diluted fermented water, wherein the weight ratio of fermented water to water is 1:4, and then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed black soldier flies aged 4-6 days. During the feeding process, if the moisture content in the feed is less than 60%, continue to add diluted fermented water so that the moisture content in the feed is not less than 60%. The specific feeding method is: on the first day, add 15 kg of plant-based material and 45 L of diluted fermented water; on the fourth day, add 10 L of diluted fermented water and turn the feed; on the fifth day, add 15 kg of plant-based material and 60 L of diluted fermented water and turn the feed; on the sixth day, turn the feed; on the seventh day, add 20 L of diluted fermented water and turn the feed; on the eighth day, add diluted fermented water 10L, turn the material; on the 9th day, add 20kg of plant-based materials and 60L of diluted fermentation water, and turn the material; on the 10th day, turn the material; on the 11th day, add 30kg of plant-based materials and 90L of water; on the 14th day, collect the insects; after collecting the insects, stir and mix the insect sand, plant-based materials and sugar to obtain a mixture, and then add diluted fermentation water to the mixture to make the moisture content of the mixture 47%, put it into a fermentation bag, and seal and ferment it at 30°C for 8 days to make the pH of the mixture ≤4.8, with a strong wine aroma, dry and crush to obtain crushed material A; dry the insects and crush to obtain crushed material B, stir and mix crushed material B with crushed material A to obtain a new fermentation-per-abdominal pre-digested protein product; wherein the weight ratio of insect sand, plant-based materials and sugar in the mixture is 50:50:3.

[0039] Performance testing Deodorization performance: Use an ammonia detector to detect the ammonia emission during the breeding process. The model is GNXMD01. The lower the ammonia emission, the smaller the odor. The average daily ammonia emission is measured at a fixed time every day. Take some larvae, wash them with tap water and wipe them dry. After the moisture on the larvae's skin evaporates, put them in the center of the ammonia detector (80cm×50cm×30cm). After the ammonia concentration has risen steadily for a period of time, start timing and record the initial value of ammonia within 10 minutes to obtain the ammonia emission value during this period. Take some substrates (depending on the level of ammonia emissions on that day) and measure the average daily ammonia emission of the substrate in the same way. This method focuses on the ammonia emission value per gram of larvae or substrate per minute. The ammonia emission rate and the cumulative ammonia emission are calculated as follows: 1ppm=0.756mg·m -3 The volume of the ammonia detector is 80cm×50cm×30cm=0.12m 3 , larvae or substrate ammonia emission rate (mg / g / min) = [0.756mg·m -3 ×0.12m -3 × ammonia emission value (ppm)] / [larva weight (g) or substrate weight (g) × time (min)]; The final unit is converted into mg / kg / day, and the value is taken as an integer multiple of ten. The smaller the cumulative ammonia emissions, the smaller the odor and the better the deodorization effect.

[0040] Growth condition: The weight of the larvae is weighed during the growth process. The larger the weight, the better the growth condition of the larvae.

[0041] Product performance testing: Determination of component content of new fermentation-pre-digested protein products and livestock and poultry breeding products.

[0042] Table 1 Determination results of ammonia during feeding Table 2 Results of determination of average weight of larvae Table 3 Performance test results of products Combined with Table 1, Table 2, Example 1 and Comparative Examples 1-2, in Example 1, the larvae have less odor during the breeding process and the larvae grow better. It can be seen that the use of plant-based materials to feed black soldier flies produces less odor, and the fermented water is used for feeding materials. The black soldier flies are harvested after 10-15 days of breeding. During this period, the material temperature rises, which can just increase the good temperature for microbial reproduction. The black soldier flies crawl to eat the material, which can fluff the material and ventilate it. The low pH of the fermented water and the continuous fermentation of the material to produce acid, enzymes and other aromatic substances can reduce the odor problem in the breeding of black soldier flies. In addition, the method in the present application can also promote the growth of black soldier flies.

[0043] Combining Table 1, Example 1 and Example 2, the larvae in Example 2 have less odor during their growth process. It can be seen that when preparing fermented water, adding molasses can provide energy and nutrition for microorganisms, improve the fermentation effect of fermented water, and thus further reduce the generation of odor.

[0044] Combining Table 1 and Examples 2-4, the larvae in Example 3 have the least odor during their growth process. This shows that when preparing fermented water, the ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based materials, and sugar in Example 3 is optimal, thereby obtaining the best fermented water.

[0045] Combining Table 1, Example 3, Example 5 and Example 6, the larvae in Example 5 have the least odor during their growth, which shows that the fermentation conditions in Example 5 are the best when preparing fermented water.

[0046] Combining Table 1, Example 5 and Example 7, the larvae in Example 5 and Example 7 have less odor during the growth process. It can be seen that when preparing materials, when the weight ratio of palm meal to cassava residue in the plant-based material is 1:4, feeding the larvae can also reduce the generation of odor.

[0047] Combining Table 1, Example 7 and Example 8, the larvae in Example 8 have less odor during the growth process. It can be seen that when preparing materials, solid-state fermentation of plant-based materials can be performed first, which can further reduce the odor problem during the black soldier fly breeding process.

[0048] Combining Table 1, Table 2, Example 1 and Example 9, the growth of the larvae in Example 9 is better, but the larvae have more odor during the breeding process. It can be seen that when preparing the base material, adding food waste to the plant-based material can further promote the growth of black soldier flies, but it will affect the odor treatment effect.

[0049] Combining Table 3, Application Example 1 and Application Example 2, it can be seen that the livestock and poultry breeding products and the new fermentation-pre-digested protein products obtained by the method of this application, such as insects, insect sand, and feed waste, all have good nutritional value.

[0050] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for removing odor in black soldier fly breeding by pre-treating plant substrates through liquid fermentation, characterized in that: The following steps are involved: Prepare base material: stir and mix the plant-based materials evenly and set aside; Preparation of fermented water: adding water and bacterial powder containing lactic acid bacteria and yeast to the plant-based material, and fermenting the mixture to obtain fermented water so that the pH of the mixture is 4.0-5.2; Feeding: adding water to the fermented water for dilution to obtain diluted fermented water, and then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75%, to obtain feed, and using the feed to feed the black soldier flies.

2. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The step of preparing the base material is: stirring and mixing the plant-based material and the kitchen waste evenly to obtain a mixed material for standby use.

3. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The plant-based material includes a mixture of palm meal and cassava residue, and the weight ratio of the palm meal to the cassava residue is 2:3 or 1:

4.

4. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The fermentation water preparation step is: adding water, bacterial powder containing lactic acid bacteria and yeast, and sugar to the plant-based material, fermenting until the pH of the mixed solution is 4.0-5.2, and obtaining the fermentation water.

5. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 4, characterized in that: In the fermentation water preparation step, the weight ratio of water, bacterial powder containing lactic acid bacteria and yeast, plant-based materials, and sugar is 500: (0.5-1): (25-100): (5-50).

6. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: In the fermentation water preparation step, the fermentation temperature is 24-40° C. and the fermentation time is 24-120 hours.

7. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: In the feeding step, when water is added to the fermented water for dilution, the weight ratio of the fermented water to the water is 1:(3-5).

8. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The step of preparing the base material is: stirring and mixing the plant-based material evenly, and then performing solid-state fermentation for standby use.

9. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The feeding step comprises: adding water to the fermented water for dilution to obtain diluted fermented water, then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed the black soldier flies; adding the plant-based material accounting for 40%-80% of the total dry weight of the fed feed 2-3 days before collecting the black soldier flies, and adjusting the moisture content of the material to 60%-75% by using the diluted fermented water; after collecting the black soldier flies, adjusting the moisture content of the feed waste to 35%-60% by using the diluted fermented water, and then directly loading it into a fermentation bag, and fermenting it at 20-40° C. for 2-15 days so that the material has a wine aroma, no odor, and a pH value of ≤4.8, and the fermentation is terminated to obtain livestock and poultry breeding products.

10. The method of removing odor in black soldier fly breeding by pretreating plant substrates through liquid fermentation according to claim 1, characterized in that: The feeding step comprises: adding water to the fermented water for dilution to obtain diluted fermented water, then adding the diluted fermented water to the plant-based material so that the moisture content of the material is 60-75% to obtain feed, and using the feed to feed the black soldier flies; after collecting the insects, stirring and mixing the insect sand, the plant-based material and sugar to obtain a mixture, then adding the diluted fermented water to the mixture so that the moisture content of the mixture is 35%-60%, putting the mixture into a fermentation bag, sealing and fermenting the mixture at 20-40° C. for 2-15 days so that the pH of the mixture is ≤4.8 and has a strong wine aroma, drying and crushing to obtain a crushed material A; drying and crushing the insects to obtain a crushed material B, stirring and mixing the crushed material B with the crushed material A to obtain a novel fermentation-per-abdominal pre-digested protein product; wherein the weight ratio of the insect sand, the plant-based material and the sugar in the mixture is (30-70): (30-70): (1-5).

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